Alejandra Vargas-Valderrama, Anne-Charlotte Ponsen, Morgane Le Gall, Denis Clay, Sébastien Jacques, Tudor Manoliu, Valérie Rouffiac, Karine Ser-le-Roux, Cyril Quivoron, Fawzia Louache, Georges Uzan, Maria-Teresa Mitjavila-Garcia, Estelle Oberlin, Hind Guenou
BACKGROUND: hPSC-derived endothelial and hematopoietic cells (ECs and HCs) are an interesting source of cells for tissue engineering. Despite their close spatial and temporal embryonic development, current hPSC differentiation protocols are specialized in only one of these lineages. In this study, we generated a hematoendothelial population that could be further differentiated in vitro to both lineages. METHODS: Two hESCs and one hiPSC lines were differentiated into a hematoendothelial population, hPSC-ECs and blast colonies (hPSC-BCs) via CD144+ -embryoid bodies (hPSC-EBs)...
June 17, 2022: Stem Cell Research & Therapy