keyword
MENU ▼
Read by QxMD icon Read
search

Hemogenic endothelium

keyword
https://www.readbyqxmd.com/read/27880904/lyve1-marks-the-divergence-of-yolk-sac-definitive-hemogenic-endothelium-from-the-primitive-erythroid-lineage
#1
Lydia K Lee, Yasamine Ghorbanian, Wenyuan Wang, Yanling Wang, Yeon Joo Kim, Irving L Weissman, Matthew A Inlay, Hanna K A Mikkola
The contribution of the different waves and sites of developmental hematopoiesis to fetal and adult blood production remains unclear. Here, we identify lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1) as a marker of yolk sac (YS) endothelium and definitive hematopoietic stem and progenitor cells (HSPCs). Endothelium in mid-gestation YS and vitelline vessels, but not the dorsal aorta and placenta, were labeled by Lyve1-Cre. Most YS HSPCs and erythro-myeloid progenitors were Lyve1-Cre lineage traced, but primitive erythroid cells were not, suggesting that they represent distinct lineages...
November 22, 2016: Cell Reports
https://www.readbyqxmd.com/read/27861498/somite-derived-retinoic-acid-regulates-zebrafish-hematopoietic-stem-cell-formation
#2
Laura M Pillay, Kacey J Mackowetzky, Sonya A Widen, Andrew Jan Waskiewicz
Hematopoietic stem cells (HSCs) are multipotent progenitors that generate all vertebrate adult blood lineages. Recent analyses have highlighted the importance of somite-derived signaling factors in regulating HSC specification and emergence from dorsal aorta hemogenic endothelium. However, these factors remain largely uncharacterized. We provide evidence that the vitamin A derivative retinoic acid (RA) functions as an essential regulator of zebrafish HSC formation. Temporal analyses indicate that RA is required for HSC gene expression prior to dorsal aorta formation, at a time when the predominant RA synthesis enzyme, aldh1a2, is strongly expressed within the paraxial mesoderm and somites...
2016: PloS One
https://www.readbyqxmd.com/read/27802172/interplay-between-sox7-and-runx1-regulates-hemogenic-endothelial-fate-in-the-yolk-sac
#3
Andrew J Lilly, Guilherme Costa, Anne Largeot, Muhammad Z H Fadlullah, Michael Lie-A-Ling, Georges Lacaud, Valerie Kouskoff
Endothelial to hematopoietic transition (EHT) is a dynamic process involving the shutting down of endothelial gene expression and switching on of hematopoietic gene transcription. Although the factors regulating EHT in hemogenic endothelium (HE) of the dorsal aorta have been relatively well studied, the molecular regulation of yolk sac HE remains poorly understood. Here, we show that SOX7 inhibits the expression of RUNX1 target genes in HE, while having no effect on RUNX1 expression itself. We establish that SOX7 directly interacts with RUNX1 and inhibits its transcriptional activity...
December 1, 2016: Development
https://www.readbyqxmd.com/read/27760216/nitric-oxide-donor-molsidomine-positively-modulates-myogenic-differentiation-of-embryonic-endothelial-progenitors
#4
Mario Tirone, Valentina Conti, Fabio Manenti, Pier Andrea Nicolosi, Cristina D'Orlando, Emanuele Azzoni, Silvia Brunelli
Embryonic VE-Cadherin-expressing progenitors (eVE-Cad+), including hemogenic endothelium, have been shown to generate hematopoietic stem cells and a variety of other progenitors, including mesoangioblasts, or MABs. MABs are vessel-associated progenitors with multilineage mesodermal differentiation potential that can physiologically contribute to skeletal muscle development and regeneration, and have been used in an ex vivo cell therapy setting for the treatment of muscular dystrophy. There is currently a therapeutic need for molecules that could improve the efficacy of cell therapy protocols; one such good candidate is nitric oxide...
2016: PloS One
https://www.readbyqxmd.com/read/27638855/evi1-regulates-notch-activation-to-induce-zebrafish-hematopoietic-stem-cell-emergence
#5
Martina Konantz, Elisa Alghisi, Joëlle S Müller, Anna Lenard, Virginie Esain, Kelli J Carroll, Lothar Kanz, Trista E North, Claudia Lengerke
During development, hematopoietic stem cells (HSCs) emerge from aortic endothelial cells (ECs) through an intermediate stage called hemogenic endothelium by a process known as endothelial-to-hematopoietic transition (EHT). While Notch signaling, including its upstream regulator Vegf, is known to regulate this process, the precise molecular control and temporal specificity of Notch activity remain unclear. Here, we identify the zebrafish transcriptional regulator evi1 as critically required for Notch-mediated EHT In vivo live imaging studies indicate that evi1 suppression impairs EC progression to hematopoietic fate and therefore HSC emergence...
November 2, 2016: EMBO Journal
https://www.readbyqxmd.com/read/27597316/origin-of-the-hematopoietic-system-in-the-human-embryo
#6
Emmanuelle Julien, Reine El Omar, Manuela Tavian
The continuous generation of blood cells throughout life relies on the existence of hematopoietic stem cells (HSC) generated during embryogenesis. Given the importance of HSC transplantation in cell-based therapeutic approaches, considerable efforts have been made toward understanding the developmental origins of embryonic HSC. Adult-type HSC are first generated in the aorta-gonad-mesonephros (AGM) region between days 27 and 40 of human embryonic development, but an elusive blood-forming potential is present earlier in the underlying splanchnopleura...
September 6, 2016: FEBS Letters
https://www.readbyqxmd.com/read/27554085/identification-of-novel-regulators-of-developmental-hematopoiesis-using-endoglin-regulatory-elements-as-molecular-probes
#7
Rabab Nasrallah, Eva M Fast, Parham Solaimani, Kathy Knezevic, Alexia Eliades, Rahima Patel, Roshana Thambyrajah, Ashwin Unnikrishnan, Julie Thoms, Dominik Beck, Chris S Vink, Aileen Smith, Jason Wong, Mairi Shepherd, David Kent, Rahul Roychoudhuri, Fabian Paul, Julia Klippert, Annette Hammes, Thomas Willnow, Bertie Göttgens, Elaine Dzierzak, Leonard I Zon, George Lacaud, Valerie Kouskoff, John E Pimanda
Enhancers are the primary determinants of cell identity, and specific promoter/enhancer combinations of Endoglin (ENG) have been shown to target blood and endothelium in the embryo. Here, we generated a series of embryonic stem cell lines, each targeted with reporter constructs driven by specific promoter/enhancer combinations of ENG, to evaluate their discriminative potential and value as molecular probes of the corresponding transcriptome. The Eng promoter (P) in combination with the -8/+7/+9-kb enhancers, targeted cells in FLK1 mesoderm that were enriched for blast colony forming potential, whereas the P/-8-kb enhancer targeted TIE2+/c-KIT+/CD41- endothelial cells that were enriched for hematopoietic potential...
October 13, 2016: Blood
https://www.readbyqxmd.com/read/27499523/transforming-growth-factor-%C3%AE-drives-hemogenic-endothelium-programming-and-the-transition-to-hematopoietic-stem-cells
#8
Rui Monteiro, Philip Pinheiro, Nicola Joseph, Tessa Peterkin, Jana Koth, Emmanouela Repapi, Florian Bonkhofer, Arif Kirmizitas, Roger Patient
Hematopoietic stem cells (HSCs) are self-renewing multipotent stem cells that generate mature blood lineages throughout life. They, together with hematopoietic progenitor cells (collectively known as HSPCs), emerge from hemogenic endothelium in the floor of the embryonic dorsal aorta by an endothelial-to-hematopoietic transition (EHT). Here we demonstrate that transforming growth factor β (TGFβ) is required for HSPC specification and that it regulates the expression of the Notch ligand Jagged1a in endothelial cells prior to EHT, in a striking parallel with the epithelial-to-mesenchymal transition (EMT)...
August 22, 2016: Developmental Cell
https://www.readbyqxmd.com/read/27399214/new-insights-into-the-regulation-by-runx1-and-gfi1-s-proteins-of-the-endothelial-to-hematopoietic-transition-generating-primordial-hematopoietic-cells
#9
Roshana Thambyrajah, Rahima Patel, Milena Mazan, Michael Lie-A-Ling, Andrew J Lilly, Alexia Eliades, Sara Menegatti, Eva Garcia-Alegria, Magdalena Florkowska, Kiran Batta, Valerie Kouskoff, Georges Lacaud
The first hematopoietic cells are generated very early in ontogeny to support the growth of the embryo and to provide the foundation to the adult hematopoietic system. There is a considerable therapeutic interest in understanding how these first blood cells are generated in order to try to reproduce this process in vitro. This would allow to generate at will blood products or hematopoietic cell populations from embryonic stem cells (ESCs), induced pluripotent stem cells or through directed reprogramming. Recent studies have clearly established that the first hematopoietic cells originate from a hemogenic endothelium (HE) through an endothelial to hematopoietic transition (EHT)...
July 11, 2016: Cell Cycle
https://www.readbyqxmd.com/read/27391301/generating-human-hematopoietic-stem-cells-in-vitro-exploring-endothelial-to-hematopoietic-transition-as-a-portal-for-stemness-acquisition
#10
REVIEW
Igor I Slukvin
Advances in cellular reprogramming technologies have created alternative platforms for the production of blood cells, either through inducing pluripotency in somatic cells or by way of direct conversion of nonhematopoietic cells into blood cells. However, de novo generation of hematopoietic stem cells (HSCs) with robust and sustained multilineage engraftment potential remains a significant challenge. Hemogenic endothelium (HE) has been recognized as a unique transitional stage of blood development from mesoderm at which HSCs arise in certain embryonic locations...
November 2016: FEBS Letters
https://www.readbyqxmd.com/read/27389484/insights-into-blood-cell-formation-from-hemogenic-endothelium-in-lesser-known-anatomic-sites
#11
Amanda D Yzaguirre, Nancy A Speck
BACKGROUND: Hematopoietic stem and progenitor cells (HSPCs) are generated de novo in the embryo in a process termed the endothelial to hematopoietic transition (EHT). EHT is most extensively studied in the yolk sac and dorsal aorta. Recently new sites of hematopoiesis have been described, including the heart, somites, head, and venous plexus of the yolk sac. RESULTS: We examined sites of HSPC formation in well-studied and in less well-known sites by mapping the expression of the key EHT factor Runx1 along with several other markers by means of confocal microscopy...
October 2016: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/27341393/isolation-of-murine-embryonic-hemogenic-endothelial-cells
#12
Jennifer S Fang, Emily C Gritz, Kathrina L Marcelo, Karen K Hirschi
The specification of hemogenic endothelial cells from embryonic vascular endothelium occurs during brief developmental periods within distinct tissues, and is necessary for the emergence of definitive HSPC from the murine extra embryonic yolk sac, placenta, umbilical vessels, and the embryonic aorta-gonad-mesonephros (AGM) region. The transient nature and small size of this cell population renders its reproducible isolation for careful quantification and experimental applications technically difficult. We have established a fluorescence-activated cell sorting (FACS)-based protocol for simultaneous isolation of hemogenic endothelial cells and HSPC during their peak generation times in the yolk sac and AGM...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27250641/ephrinb2-regulates-the-emergence-of-a-hemogenic-endothelium-from-the-aorta
#13
Inn-Inn Chen, Arianna Caprioli, Hidetaka Ohnuki, Hyeongil Kwak, Catherine Porcher, Giovanna Tosato
Adult-type intraembryonic hematopoiesis arises from specialized endothelial cells of the dorsal aorta (DA). Despite the critical importance of this specialized endothelium for establishment of hematopoietic stem cells and adult hematopoietic lineages, the mechanisms regulating its emergence are incompletely understood. We show that EphrinB2, a principal regulator of endothelial cell function, controls the development of endothelium producing adult-type hematopoiesis. The absence of EphrinB2 impairs DA-derived hematopoiesis...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27239041/the-hemogenic-competence-of-endothelial-progenitors-is-restricted-by-runx1-silencing-during-embryonic-development
#14
Alexia Eliades, Sarah Wareing, Elli Marinopoulou, Muhammad Z H Fadlullah, Rahima Patel, Joanna B Grabarek, Berenika Plusa, Georges Lacaud, Valerie Kouskoff
It is now well-established that hematopoietic stem cells (HSCs) and progenitor cells originate from a specialized subset of endothelium, termed hemogenic endothelium (HE), via an endothelial-to-hematopoietic transition. However, the molecular mechanisms determining which endothelial progenitors possess this hemogenic potential are currently unknown. Here, we investigated the changes in hemogenic potential in endothelial progenitors at the early stages of embryonic development. Using an ETV2::GFP reporter mouse to isolate emerging endothelial progenitors, we observed a dramatic decrease in hemogenic potential between embryonic day (E)7...
June 7, 2016: Cell Reports
https://www.readbyqxmd.com/read/27227884/lack-of-phenotypical-and-morphological-evidences-of-endothelial-to-hematopoietic-transition-in-the-murine-embryonic-head-during-hematopoietic-stem-cell-emergence
#15
Kazuhide Iizuka, Tomomasa Yokomizo, Naoki Watanabe, Yosuke Tanaka, Motomi Osato, Tomoiku Takaku, Norio Komatsu
During mouse ontogeny, hematopoietic cells arise from specialized endothelial cells, i.e., the hemogenic endothelium, and form clusters in the lumen of arterial vessels. Hemogenic endothelial cells have been observed in several embryonic tissues, such as the dorsal aorta, the placenta and the yolk sac. Recent work suggests that the mouse embryonic head also produces hematopoietic stem cells (HSCs)/progenitors. However, a histological basis for HSC generation in the head has not yet been determined because the hematopoietic clusters and hemogenic endothelium in the head region have not been well characterized...
2016: PloS One
https://www.readbyqxmd.com/read/27137656/taking-the-leap-runx1-in-the-formation-of-blood-from-endothelium
#16
J Tober, M W Maijenburg, N A Speck
Blood cell formation in the embryo occurs from multiple anatomic sites and results in the production of hematopoietic stem and progenitor cells that appear in overlapping waves. The transcription factor Runx1 is involved in a dramatic step of this process, for the transition from an endothelial cell that is integrated in a monolayer to a nonadherent circulating blood cell, a process conceptually similar to the epithelial to mesenchymal cell transition. Here we will review the role of Runx1 in the so-called hemogenic endothelium...
2016: Current Topics in Developmental Biology
https://www.readbyqxmd.com/read/27117782/cyclic-amp-signaling-through-epac-axis-modulates-human-hemogenic-endothelium-and-enhances-hematopoietic-cell-generation
#17
Shobhit Saxena, Roger E Rönn, Carolina Guibentif, Roksana Moraghebi, Niels-Bjarne Woods
Hematopoietic cells emerge from hemogenic endothelium in the developing embryo. Mechanisms behind human hematopoietic stem and progenitor cell development remain unclear. Using a human pluripotent stem cell differentiation model, we report that cyclic AMP (cAMP) induction dramatically increases HSC-like cell frequencies. We show that hematopoietic cell generation requires cAMP signaling through the Exchange proteins activated by cAMP (cAMP-Epac) axis; Epac signaling inhibition decreased both hemogenic and non-hemogenic endothelium, and abrogated hematopoietic cell generation...
May 10, 2016: Stem Cell Reports
https://www.readbyqxmd.com/read/27105579/extravascular-endothelial-and-hematopoietic-islands-form-through-multiple-pathways-in-midgestation-mouse-embryos
#18
Amanda D Yzaguirre, Nancy A Speck
The de novo generation of hematopoietic cells occurs during midgestation when a population of endothelial cells called hemogenic endothelium transitions into hematopoietic progenitors and stem cells. In mammalian embryos, the newly formed hematopoietic cells form clusters in the lumens of the major arteries in the embryo proper and in the vascular plexus of the yolk sac. Small clusters of hematopoietic cells that are independent of the vasculature (referred to here as extravascular islands) were shown to form in the mesentery during vascular remodeling of the vitelline artery...
July 1, 2016: Developmental Biology
https://www.readbyqxmd.com/read/27060598/pdgfrb-cre-targets-lymphatic-endothelial-cells-of-both-venous-and-non-venous-origins
#19
Maria H Ulvmar, Ines Martinez-Corral, Lukas Stanczuk, Taija Mäkinen
The Pdgfrb-Cre line has been used as a tool to specifically target pericytes and vascular smooth muscle cells. Recent studies showed additional targeting of cardiac and mesenteric lymphatic endothelial cells (LECs) by the Pdgfrb-Cre transgene. In the heart, this was suggested to provide evidence for a previously unknown nonvenous source of LECs originating from yolk sac (YS) hemogenic endothelium (HemEC). Here we show that Pdgfrb-Cre does not, however, target YS HemEC or YS-derived erythro-myeloid progenitors (EMPs)...
June 2016: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/26954547/hematopoietic-reprogramming-in-vitro-informs-in-vivo-identification-of-hemogenic-precursors-to-definitive-hematopoietic-stem-cells
#20
Carlos-Filipe Pereira, Betty Chang, Andreia Gomes, Jeffrey Bernitz, Dmitri Papatsenko, Xiaohong Niu, Gemma Swiers, Emanuele Azzoni, Marella F T R de Bruijn, Christoph Schaniel, Ihor R Lemischka, Kateri A Moore
Definitive hematopoiesis emerges via an endothelial-to-hematopoietic transition in the embryo and placenta; however, the precursor cells to hemogenic endothelium are not defined phenotypically. We previously demonstrated that the induction of hematopoietic progenitors from fibroblasts progresses through hemogenic precursors that are Prom1(+)Sca1(+)CD34(+)CD45(-) (PS34CD45(-)). Guided by these studies, we analyzed mouse placentas and identified a population with this phenotype. These cells express endothelial markers, are heterogeneous for early hematopoietic markers, and localize to the vascular labyrinth...
March 7, 2016: Developmental Cell
keyword
keyword
24034
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"