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https://www.readbyqxmd.com/read/28929967/-results-of-a-multicenter-study-investigating-plasmid-mediated-colistin-resistance-genes-mcr-1-and-mcr-2-in-clinical-enterobacteriaceae-%C3%A4-solates-from-turkey
#1
Ayşe Nur Sarı, Serap Süzük, Onur Karatuna, Dilara Öğünç, Ayşe Esra Karakoç, Zeynep Çizmeci, Hikmet Eda Alışkan, Füsun Cömert, Mustafa Zahir Bakıcı, Nezahat Akpolat, Fatma Feriha Çilli, Yasemin Zer, Aysel Karataş, Bahar Akgün Karapınar, Gülçin Bayramoğlu, Melda Özdamar, Fatma Kalem, Nuran Delialioğlu, Elif Aktaş, Nisel Yılmaz, Şaban Gürcan, Zeynep Gülay
Colistin is a polymyxin antibiotic which is considered as one of the last line agents against infections due to multidrug resistant or carbapenem resistant gram-negative pathogens. Colistin resistance is associated with chromosomal alterations which can usually cause mutations in genes coding specific two component regulator systems. The first plasmid-mediated colistin resistance gene, mcr-1 was described in Escherichia coli and Klebsiella pneumoniae isolates in December 2015 and followed by another plasmid-mediated colistin resistance gene mcr-2 in 2016...
July 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28920790/pharmacokinetics-efficacy-and-safety-of-voriconazole-and-itraconazole-in-healthy-cottonmouths-agkistrodon-piscivorus-and-massasauga-rattlesnakes-sistrurus-catenatus-with-snake-fungal-disease
#2
Dana M Lindemann, Matthew C Allender, Marta Rzadkowska, Grace Archer, Lauren Kane, Eric Baitchman, Elizabeth A Driskell, Caroline T Chu, Kuldeep Singh, Shih-Hsuan Hsiao, John M Sykes, Sherry Cox
Snake fungal disease (SFD; Ophidiomyces ophiodiicola) is posing a significant threat to several free-ranging populations of pitvipers. Triazole antifungals have been proposed for the treatment of mycoses in reptiles; however, data are lacking about their safety and efficacy in snakes with SFD. Study 1 investigated in vitro susceptibility, and identified that plasma concentrations >250 ng/ml (voriconazole) and >1,000 ng/ml (itraconazole) may be effective in vivo for SFD. In Study 2, the pharmacokinetics after a single subcutaneous voriconazole injection were assessed in apparently healthy free-ranging cottonmouths (Agkistrodon piscivorus)...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28919348/prevalence-of-colistin-resistance-in-clinical-isolates-of-enterobacteriaceae-a-four-year-cross-sectional-study
#3
Núria Prim, Miquel Turbau, Alba Rivera, Judith Rodríguez-Navarro, Pere Coll, Beatriz Mirelis
Objectives The objectives were to determine the prevalence of colistin resistance in clinical isolates of Enterobacteriaceae, and to gain knowledge on the epidemiological and clinical features of the patients. Methods All colistin-resistant Enterobacteriaceae consecutively isolated from clinical samples in our institution from 2012 to 2015, were included in this cross-sectional study. Intrinsic-resistant species with were excluded. Minimum inhibitory concentration was performed by gradient diffusion. Detection of plasmid-encoded colistin resistance genes mcr-1 and mcr-2 was performed by amplification...
September 14, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28912313/complete-genome-sequence-of-a-shiga-toxin-producing-enterobacter-cloacae-clinical-isolate
#4
Michelle Qiu Carter, Antares Pham, Steven Huynh, Xiaohua He
Enterobacter cloacae strain M12X01451 was isolated from a patient with mild diarrhea. This strain produces a novel subtype of Shiga toxin 1, Stx1e. The Stx1e-converting prophage in strain M12X01451 is stable and can infect other bacteria following induction. Here we report the complete genome sequence and annotation of strain M12X01451.
September 14, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28893435/gradual-bone-transfer-for-the-correction-of-the-pubic-diastasis-using-the-ilizarov-technique-in-closure-of-bladder-and-cloacal-exstrophy
#5
Hiroshi Kusakabe, Katsuhiko Ueoka, Shinichiro Takayama, Atsuhito Seki
BACKGROUND: In the patient of the cloacal exstrophy, cloaca with local abdominal wall is disrupted and exposed inner surface of the bladder needs early closure. Pelvic osteotomies are required for severe cases whose bladder cannot be closed by the suture of soft tissue only. We developed a technique involving the gradual positioning of bone fragments using a light, Ilizarov external fixator. The usefulness of the technique was assessed. METHODS: We enrolled 3 patients with cloacal exstrophy and 1 with bladder exstrophy as a gradual transfer group and 6 patients who were treated by other osteotomies as a control group...
September 8, 2017: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/28892921/a-rare-case-of-genital-malformation-with-omphalocele-exstrophy-of-bladder-imperforate-anus-and-spinal-defect-complex-autopsy-findings
#6
K Mamatha, B R Yelikar, Varsha R Deshpande, B S Disha
Omphalocele, Exstrophy of cloaca, Imperforate anus and Spinal defects (OEIS) is a severe manifestation of exstrophy-epispadias sequence with a combination of defects including OEIS. It results from improper closure of anterior abdominal wall and defective development of cloaca and urogenital septum due to defect in blastogenesis during the 4(th) week of gestation. Identification of this complex is important through foetal autopsy as this condition can recur in siblings. Prenatal diagnosis also helps to prevent foetal death with appropriate management in the less severe cases...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28888386/pediatric-chronic-osteomyelitis-in-the-outskirts-of-al-ushbuna-carnide-lisboa-portugal
#7
Ana Tavares, Calil Makhoul, Mário Monteiro, Francisco Curate
The skeletal remains of seven individuals (five non-adults and two adults) were recovered during an archeological intervention in the township of Carnide (Lisbon, Portugal). Funerary anthropology strongly suggests that the sample is from the Medieval Islamic period (8th - 12th centuries AD). This report presents a case of chronic osteomyelitis in a non-adult individual. The diagnostic is substantiated by the presence of pathognomonic signs of osteomyelitis, including the presence of cloacae and a sequestrum in the left tibia...
September 2017: International Journal of Paleopathology
https://www.readbyqxmd.com/read/28886559/sewage-treatment-effluents-in-delhi-a-key-contributor-of-%C3%AE-lactam-resistant-bacteria-and-genes-to-the-environment
#8
Manisha Lamba, Shaikh Ziauddin Ahammad
Rapid emergence of antibiotic resistance (AR) in developing countries is posing a greater health risk and increasing the global disease burden. Lack of access to safe drinking water, poor sanitation and inadequate sewage treatment facilities in these countries are fueling the problem associated with emergence of AR. Rapid proliferation of AR mediated by treated and untreated discharges from sewage treatment plants (STPs) is a prime public health concern. This study aims to understand the occurrence, fate, and routes of proliferation of carbapenem (KPC) and extended spectrum β-lactam (ESBL) resistant bacteria, and selected resistant genes in the samples collected from different unit operations in 12 STPs in New Delhi over two seasons...
August 28, 2017: Chemosphere
https://www.readbyqxmd.com/read/28886013/secondary-bacterial-infections-and-antibiotic-resistance-among-tungiasis-patients-in-western-kenya
#9
Ruth Monyenye Nyangacha, David Odongo, Florence Oyieke, Missiani Ochwoto, Richard Korir, Ronald Kiprotich Ngetich, Gladys Nginya, Olipher Makwaga, Christine Bii, Peter Mwitari, Festus Tolo
Tungiasis or jigger infestation is a parasitic disease caused by the female sand flea Tunga penetrans. Secondary infection of the lesions caused by this flea is common in endemic communities. This study sought to shed light on the bacterial pathogens causing secondary infections in tungiasis lesions and their susceptibility profiles to commonly prescribed antibiotics. Participants were recruited with the help of Community Health Workers. Swabs were taken from lesions which showed signs of secondary infection...
September 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28885139/prevalence-of-fosfomycin-resistance-and-plasmid-mediated-fosfomycin-modifying-enzymes-among-carbapenem-resistant-enterobacteriaceae-in-zhejiang-china
#10
Lin Huang, Yan Yan Hu, Rong Zhang
Two hundred and thirty-three nonduplicated clinical isolates of carbapenem-resistant Enterobacteriaceae were collected from four hospitals in Zhejiang, China. 45.1 % (105/233) strains were resistant to fosfomycin, among which plasmid-mediated fosfomycin-modifying enzymes fosA, fosA2, fosA3 and fosA5 were positive, and the other fos genes were negative. 80 % (12/15) Enterobacter cloacae isolates were positive for fosA. 100 % (73/73) Klebsiella pneumoniae isolates were positive for fosA5. A conjugation experiment indicated that fosfomycin resistance could be transferred to an Escherichia coli recipient strain successfully...
September 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28883810/from-farms-to-markets-gram-negative-bacteria-resistant-to-third-generation-cephalosporins-in-fruits-and-vegetables-in-a-region-of-north-africa
#11
Ferielle Mesbah Zekar, Sophie A Granier, Muriel Marault, Lydia Yaici, Benoit Gassilloud, Charles Manceau, Abdelaziz Touati, Yves Millemann
The role of food in human exposure to antimicrobial-resistant bacteria is a growing food safety issue. The contribution of fruits and vegetables eaten raw to this exposure is still unclear. The evaluation of contamination levels of fruits, vegetables and the agricultural environment by third-generation cephalosporin (3GC)-resistant Gram-negative bacteria was performed by analyzing 491 samples of fruits and vegetables collected from 5 markets and 7 farms in Bejaia area, north-eastern Mediterranean coast of Algeria...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28882783/ramr-is-not-involved-in-the-regulation-of-rama-associated-antibiotic-resistance-in-salmonella-enterica-serovar-typhi
#12
Minhui Miao, Wei Cao, Hongxia Wang, Jieting Yan, Min Wang, Yi Zheng, Xiaofang Xie, Xia Zhang, Haifang Zhang, Hong Du
RamA, a global transcriptional activator, belongs to the AraC/XylS family of regulatory proteins and can regulate multidrug resistance through activating the expression of AcrAB. In Salmonella spp., Klebsiella pneumonia, Enterobacter cloacae and Enterobacter aerogenes, RamR represses the transcription of gene ramA through binding to the upstream sequences of ramA. In this study, we found that the locus and transcription directions of ramA-ramR in S. Typhi GIFU10007 are different from that in S. Typhimurium (SL1344)...
September 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28881886/monitoring-sputum-culture-in-resected-esophageal-cancer-patients-with-preoperative-treatment
#13
K Kosumi, Y Baba, K Yamashita, T Ishimoto, K Nakamura, M Ohuchi, Y Kiyozumi, D Izumi, R Tokunaga, K Harada, H Shigaki, J Kurashige, M Iwatsuki, Y Sakamoto, N Yoshida, M Watanabe, H Baba
Pneumonia is a major cause of postesophagectomy mortality and worsens the long-term survival in resected esophageal cancer patients. Moreover, preoperative treatments such as chemotherapy or chemoradiotherapy (which have recently been applied worldwide) might affect the bacterial flora of the sputum. To investigate the association among preoperative treatments, the bacterial flora of sputum, and the clinical and pathological features in resected esophageal cancer patients, this study newly investigates the effect of preoperative treatments on the bacterial flora of sputum...
December 1, 2017: Diseases of the Esophagus: Official Journal of the International Society for Diseases of the Esophagus
https://www.readbyqxmd.com/read/28878306/culture-independent-metagenomics-supports-discovery-of-uncultivable-bacteria-within-the-genus-chlamydia
#14
Alyce Taylor-Brown, Labolina Spang, Nicole Borel, Adam Polkinghorne
Advances in culture-independent methods have meant that we can more readily detect and diagnose emerging infectious disease threats in humans and animals. Metagenomics is fast becoming a popular tool for detection and characterisation of novel bacterial pathogens in their environment, and is particularly useful for obligate intracellular bacteria such as Chlamydiae that require labour-intensive culturing. We have used this tool to investigate the microbial metagenomes of Chlamydia-positive cloaca and choana samples from snakes...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28877681/fibronectin-glomerulopathy-complicated-with-persistent-cloaca-and-congenital-esophageal-atresia-a-case-report-and-literature-review
#15
Misaki Takii, Takaichi Suehiro, Aya Shima, Hideki Yotsueda, Satoshi Hisano, Ritsuko Katafuchi
BACKGROUND: Fibronectin glomerulopathy is a rare, inherited, autosomal dominant, glomerular disease characterized by proteinuria, microscopic hematuria, hypertension, massive glomerular deposits of fibronectin, and slow progression to end-stage renal failure. Because the incident of fibronectin glomerulopathy is extremely low, the pathophysiology, genetic abnormalities, epidemiology, and mechanisms remain to be elucidated. CASE PRESENTATION: We report a 21-year-old woman with fibronectin glomerulopathy, who had been diagnosed with persistent cloaca and congenital esophageal atresia at birth...
September 6, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28877672/chronic-lower-extremity-wound-infection-due-to-kerstersia-gyiorum-in-a-patient-with-buerger-s-disease-a-case-report
#16
Irmak Baran, Arife Polat Düzgün, İpek Mumcuoğlu, Neriman Aksu
BACKGROUND: Kerstersia gyiorum is an extremely rare pathogen of human infection. It can cause chronic infection in patients with underlying conditions. It can easily be misdiagnosed if proper diagnostic methods are not used. CASE PRESENTATION: A 47-year-old male patient with a history of Buerger's Disease for 28 years presented to our hospital with an infected chronic wound on foot. The wound was debrided, and the specimen was sent to Microbiology laboratory. Gram staining of the specimen showed abundant polymorphonuclear leukocytes and gram-negative bacilli...
September 6, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28877183/quantitative-proteomics-analysis-reveals-the-tolerance-of-wheat-to-salt-stress-in-response-to-enterobacter-cloacae-sbp-8
#17
Rajnish Prakash Singh, Ashish Runthala, Shahid Khan, Prabhat Nath Jha
Salinity stress adversely affects the plant growth and is a major constraint to agriculture. In the present study, we studied the role of plant growth promoting rhizobacterium (PGPR) Enterobacter cloacae SBP-8 possessing ACC deaminase activity on proteome profile of wheat (Triticum aestivum L.) under high salinity (200 mM NaCl) stress. The aim of study was to investigate the differential expressed protein in selected three (T-1, T-2, T-3) treatments and absolute quantification (MS/MS analysis) was used to detect statistically significant expressed proteins...
2017: PloS One
https://www.readbyqxmd.com/read/28876168/complete-sequence-of-pcy-ctx-a-plasmid-carrying-a-phage-like-region-and-an-isecp1-mediated-tn2-element-from-enterobacter-cloacae
#18
Zhenbo Xu, Jinhong Xie, Ling Yang, Dingqiang Chen, Brian M Peters, Mark E Shirtliff
A plasmid pCY-CTX carrying a phage-like backbone from an extensively drug-resistant Enterobacter cloacae strain Guangzhou-ECL001 (previously known as CY01) was identified in this study. By Illumina MiSeq 2 × 250-bp paired-end sequencing, de novo assembly, and PCR, full sequence of pCY-CTX was obtained. Plasmid pCY-CTX was a circular plasmid with a length of 116,700 bp, harboring 136 putative open reading frames with the average G + C content of 50.8%. The backbone of pCY-CTX showed high identity to previously reported phage-like plasmid pHCM2 and phage SSU5...
September 6, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28871553/treatment-with-granulocyte-colony-stimulating-factor-in-the-refeeding-phase-of-anorexia-nervosa-complicated-with-severe-neutropenia-and-sepsis-a-case-report
#19
Haruki Komatsu, Karin Hayashi, Fukiko Higashiyama
Leukopenia is frequently observed in patients with anorexia nervosa (AN). However, serious infectious diseases are infrequent in patients with AN. We present the case of a 13-year-old girl with AN complicated with severe neutropenia and sepsis upon refeeding. Her body mass index was 11 kg/m(2) and her absolute neutrophil count (ANC) was 1555/µL on admission. After the initiation of refeeding, her ANC gradually declined to 346/µL. High fever occurred and a blood culture tested positive for Enterobacter cloacae...
September 4, 2017: Eating and Weight Disorders: EWD
https://www.readbyqxmd.com/read/28861525/impacts-of-penicillin-binding-protein-2-inactivation-on-%C3%AE-lactamase-expression-and-muropeptide-profile-in-stenotrophomonas-maltophilia
#20
Yi-Wei Huang, Yu Wang, Yun Lin, Chin Lin, Yi-Tsung Lin, Cheng-Chih Hsu, Tsuey-Ching Yang
Penicillin binding proteins (PBPs) are involved in peptidoglycan synthesis, and their inactivation is linked to β-lactamase expression in ampR-β-lactamase module-harboring Gram-negative bacteria. There are seven annotated PBP genes, namely, mrcA, mrcB, pbpC, mrdA, ftsI, dacB, and dacC, in the Stenotrophomonas maltophilia genome, and these genes encode PBP1a, PBP1b, PBP1c, PBP2, PBP3, PBP4, and PBP6, respectively. In addition, S. maltophilia harbors two β-lactamase genes, L1 and L2, whose expression is induced via β-lactam challenge...
July 2017: MSystems
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