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https://www.readbyqxmd.com/read/28221856/enumeration-and-identification-of-coliform-bacteria-injured-by-chlorine-or-fungicide-mixed-with-agricultural-water
#1
Hidemi Izumi, Yuji Nakata, Ayano Inoue
Chemical sanitizers may induce no injury (bacteria survive), sublethal injury (bacteria are injured), or lethal injury (bacteria die). The proportion of coliform bacteria that were injured sublethally by chlorine and fungicide mixed with agricultural water (pond water), which was used to dilute the pesticide solution, was evaluated using the thin agar layer (TAL) method. In pure cultures of Enterobacter cloacae , Escherichia coli , and E. coli O157:H7 (representing a human pathogen), the percentage of chlorine-injured cells was 69 to 77% for dilute electrolyzed water containing an available chlorine level of 2 ppm...
October 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28216021/prevalence-antibiotic-susceptibility-and-characterization-of-antibiotic-resistant-genes-among-carbapenem-resistant-gram-negative-bacilli-and-yeast-in-intestinal-flora-of-cancer-patients-in-north-lebanon
#2
Rima Christophy, Marwan Osman, Hassan Mallat, Marcel Achkar, Azzam Ziedeh, Walid Moukaddem, Fouad Dabboussi, Monzer Hamze
The emergence and spread of carbapenem-resistant bacteria are a significant clinical and public health concern. The aim of the study is to determine the prevalence of intestinal carriage of carbapenem-resistant bacteria and yeasts in cancer patients under chemotherapy. 41 stool samples collected from cancer patients in Nini hospital in Tripoli, North Lebanon have been analyzed. After isolating yeasts and carbapenem-resistant bacteria, a biochemical identification and antimicrobial susceptibility profile were determined...
February 15, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28213040/the-locus-of-heat-resistance-lhr-mediates-heat-resistance-in-salmonella-enterica-escherichia-coli-and-enterobacter-cloacae
#3
Ryan G Mercer, Brian D Walker, Xianqin Yang, Lynn M McMullen, Michael G Gänzle
Enterobacteriaceae comprise food spoilage organisms as well as food-borne pathogens including Escherichia coli. Heat resistance in E. coli was attributed to a genomic island called the locus of heat resistance (LHR). This genomic island is also present in several other genera of Enterobacteriaceae, but its function in the enteric pathogens Salmonella enterica and Enterobacter cloacae is unknown. This study aimed to determine the frequency of the LHR in food isolates of E. coli, and its influence on heat resistance in S...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28198170/antibacterial-effect-of-sevoflurane-and-isoflurane
#4
M Martínez-Serrano, M Gerónimo-Pardo, A Martínez-Monsalve, M D Crespo-Sánchez
OBJECTIVE: Multidrug resistant bacteria are increasing worldwide and therapeutic options are limited. Some anaesthetics have shown antibacterial activity before. In this study, we have investigated the antibacterial effect of the halogenated anaesthetic agents sevoflurane and isoflurane against a range of resistant pathogens. METHODS: Two experiments were conducted. In the first, bacterial suspensions of both ATCC and resistant strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were exposed to liquid sevoflurane and isoflurane during 15, 30 and 60 minutes...
February 15, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/28197647/the-overproduction-of-indole-3-acetic-acid-iaa-in-endophytes-upregulates-nitrogen-fixation-in-both-bacterial-cultures-and-inoculated-rice-plants
#5
Roberto Defez, Anna Andreozzi, Carmen Bianco
Endophytic bacteria from roots and leaves of rice plants were isolated and identified in order to select the diazotrophs and improve their nitrogen-fixing abilities. The nitrogen-fixing endophytes were identified by PCR amplification of the nifH gene fragment. For this purpose, two isolates, Enterobacter cloacae RCA25 and Klebsiella variicola RCA26, and two model bacteria (Herbaspirillum seropedicae z67 and Sinorhizobium fredii NGR234) were transformed to increase the biosynthesis of the main plant auxin indole-3-acetic acid (IAA)...
February 14, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28197140/molecular-characteristics-of-carbapenem-resistant-enterobacter-cloacae-in-ningxia-province-china
#6
Zhiyun Shi, Huizheng Zhao, Gang Li, Wei Jia
The emergence of carbapenem-resistant Enterobacteriaceae (CRE) has become a major public health concern worldwide and a new challenge in the treatment of infectious diseases. The molecular characteristics of Enterobacter cloacae in Ningxia China are unknown. In this study, we reported 10 carbapenem-resistant E. cloacae isolates from the General Hospital of Ningxia Medical University, the largest university hospital in Ningxia between January 2012 and December 2013. Bacteria isolates were identified by Vitek2 compact and the identity of non-duplicate E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28192045/radio-transmitter-implantation-and-movement-in-the-wild-timber-rattlesnake-crotalus-horridus
#7
Vanessa Hale, Brian MacGowan, Lorraine Corriveau, David Huse, Andrea F T Currylow, Steve Thompson
Radiotelemetry transmitters have become critical to studies of wildlife ecology. However, little is known about how transmitter implantation surgery affects the mobility of some species, including the timber rattlesnake, Crotalus horridus . Tracking snake movement can provide insights into the effects of transmitter implantation. During 2007-2011, 71 radio transmitters were surgically implanted intracoelomically in 47 timber rattlesnakes. Over 20 of these snakes underwent surgery at least twice in 5 yr to replace old transmitters...
February 13, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28191911/mutations-of-myh14-are-associated-to-anorectal-malformations-with-recto-perineal-fistulas-in-a-small-subset-of-chinese-population
#8
Zhongxian Zhu, Lei Peng, Guanglin Chen, Weiwei Jiang, Ziyang Shen, Chunxia Du, Rujin Zang, Yang Su, Hua Xie, Hongxing Li, Yankai Xia, Weibing Tang
Anorectal malformations (ARMs) are among the most commonly congenital abnormalities of distal hindgut development, ranging from anal stenosis to anal atresia with or without fistulas and persistent cloaca. The etiology remains elusive for most ARM cases and the majority of genetic studies on ARMs were based on a candidate gene approach. Here, we first performed whole-exome sequencing in a non-consanguineous Chinese family and the result revealed a homozygous mutation (GenBank: NM_001077186; c.5393C>A[p.Ala1806Asp]) in MYH14, which encodes one of the NM II heavy chain (NMHC II) proteins playing vital roles in cell adhesion and migration...
February 13, 2017: Clinical Genetics
https://www.readbyqxmd.com/read/28190396/in-vitro-screening-of-an-fda-approved-library-against-eskape-pathogens
#9
Waleed Younis, Ahmed AbdelKhalek, Abdelrahman S Mayhoub, Mohamed N Seleem
Bacterial resistance to conventional antibiotics is an increasingly serious threat to public health worldwide that requires immediate exploration and the development of novel antimicrobial compounds. Drug repurposing is an inexpensive and untapped source of new antimicrobial leads, and it holds many attractive features warranting further attention for antimicrobial drug discovery. In an effort to repurpose drugs and explore new leads in the field of antimicrobial drug discovery, we performed a whole-cell screening assay of 1,600 Food and Drug Administration (FDA) approved drugs against Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter cloacae (ESKAPE) pathogens...
February 9, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28177186/characterization-of-enterococcus-faecalis-isolated-from-the-cloaca-of-fancy-breeds-and-confined-chickens
#10
Steffen Lynge Jørgensen, Louise Ladefoged Poulsen, Lotte Thorndal, Amir Atabak Ronaghinia, Magne Bisgaard, Henrik Christensen
AIMS: The aim was to investigate the intestinal community of E. faecalis from healthy confined non-industrialized chicken. METHODS AND RESULTS: A total of 206 E. faecalis isolates were collected from cloacal swabs. The prevalence of E. faecalis from two confined flocks, were 83% and 96%, while only 44% and 13% in two fancy breeder flocks. A total of 204 isolates were characterized by pulsed-field-gel-electrophoresis (PFGE) where 40 strains were selected for multi locus sequence typing (MLST)...
February 8, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28174569/identification-and-whole-genome-sequencing-of-the-first-case-of-kosakonia-radicincitans-causing-a-human-bloodstream-infection
#11
Micah D Bhatti, Awdhesh Kalia, Pranoti Sahasrabhojane, Jiwoong Kim, David E Greenberg, Samuel A Shelburne
The taxonomy of Enterobacter species is rapidly changing. Herein we report a bloodstream infection isolate originally identified as Enterobacter cloacae by Vitek2 methodology that we found to be Kosakonia radicincitans using genetic means. Comparative whole genome sequencing of our isolate and other published Kosakonia genomes revealed these organisms lack the AmpC β-lactamase present on the chromosome of Enterobacter sp. A fimbriae operon primarily found in Escherichia coli O157:H7 isolates was present in our organism and other available K...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28169182/reproductive-disorders-of-marsupials
#12
REVIEW
Cathy A Johnson-Delaney, Angela M Lennox
Marsupial reproduction differs significantly in anatomy and physiology from that of placental mammals. The gastrointestinal and urogenital tracts empty into a cloaca. Reproductive parameters include gestation and pouch time, as the fetus develops outside of the uterus. Captive marsupials discussed include sugar gliders), short-tailed opossums, Virginia opossums, and Tammar and Bennett's wallabies. Common disease conditions include joey eviction, pouch infections, mastitis, metritis, prostatitis, penile necrosis, and neoplasia...
February 4, 2017: Veterinary Clinics of North America. Exotic Animal Practice
https://www.readbyqxmd.com/read/28167555/global-molecular-epidemiology-of-imp-producing-enterobacteriaceae
#13
Yasufumi Matsumura, Gisele Peirano, Mary R Motyl, Mark D Adams, Liang Chen, Barry Kreiswirth, Rebekah DeVinney, Johann D D Pitout
International data on the molecular epidemiology of Enterobacteriaceae with IMP carbapenemases are lacking. We performed short read (Illumina) whole genomic sequencing on a global collection of 38 IMP-producing clinical Enterobacteriaceae (2008-14). IMP-producing Enterobacteriaceae (7 varieties within 11 class 1 integrons) were mainly present in South Pacific and Asia. Specific blaIMP containing integrons (In809 with blaIMP-4, In722 with blaIMP-6, In687 with blaIMP-14) were circulating among different bacteria in countries such as Australia, Japan and Thailand...
February 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28161293/molecular-epidemiology-of-carbapenemase-producing-enterobacteriaceae-in-a-primary-care-hospital-in-japan-2010-2013
#14
Yuki Ohno, Akihiro Nakamura, Eriko Hashimoto, Hiroko Matsutani, Noriyuki Abe, Saori Fukuda, Kohno Hisashi, Masaru Komatsu, Fumihiko Nakamura
Recently, carbapenemase-producing Enterobacteriaceae (CPE) have been spreading worldwide and have become a threat in healthcare systems. We investigated the isolation frequency and molecular epidemiological characteristics of CPE isolated from clinical samples collected at a primary care hospital over the four years of 2010-2013 in Japan. CPE were detected in 17 (0.34%) of 4875 isolates by the broth microdilution method, sodium mercaptoacetate inhibition test, and modified Hodge test using meropenem disks. The frequency of CPE isolates was 0...
February 1, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28148263/potency-and-selectivity-indices-of-acetone-leaf-extracts-of-nine-selected-south-african-trees-against-six-opportunistic-enterobacteriaceae-isolates-from-commercial-chicken-eggs
#15
Ishaku L Elisha, Alexander R Jambalang, Francien S Botha, Elna M Buys, Lyndy J McGaw, Jacobus N Eloff
BACKGROUND: The rise in antimicrobial resistance in a plethora of nosocomial and opportunistic bacterial pathogens often isolated from commercial eggs, poses a serious public health concern. The existence of these contaminants may also serve as a drawback in the current efforts of improving the well-being of immunocompromised patients. The aim of this study was to determine the efficacy of plant extracts that had good activity on Escherichia coli in previous word on pathogens isolated from eggs for possible use in combating pathogens from eggs...
February 2, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28143659/enantio-convergent-biotransformation-of-o-o-dimethyl-4-oxoazetidin-2-ylphosphonate-using-fungal-cells-of-penicillium-minioluteum-and-purified-enzymes
#16
Natalia Kmiecik, Ewa Żymańczyk-Duda
This report presents the bioconversion of O,O-dimethyl-4-oxoazetidin-2-ylphosphonate 1 performed in two ways: with the enzymatic system of P. minioluteum and with the application of purified enzymes: penicillinase and two proteases of different origin. Recorded NMR spectra allowed confirming the reaction progress and also postulating possible mechanism of conversion. The path of bioconversion was defined as enantio convergent process for both modes of applied biocatalysts. This means that kinetically driven resolution of racemic mixture of the substrate leads to the one enantiomer of the product...
January 24, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28139431/prevalence-risk-factors-outcomes-and-molecular-epidemiology-of-mcr-1-positive-enterobacteriaceae-in-patients-and-healthy-adults-from-china-an-epidemiological-and-clinical-study
#17
Yang Wang, Guo-Bao Tian, Rong Zhang, Yingbo Shen, Jonathan M Tyrrell, Xi Huang, Hongwei Zhou, Lei Lei, Hong-Yu Li, Yohei Doi, Ying Fang, Hongwei Ren, Lan-Lan Zhong, Zhangqi Shen, Kun-Jiao Zeng, Shaolin Wang, Jian-Hua Liu, Congming Wu, Timothy R Walsh, Jianzhong Shen
BACKGROUND: The mcr-1 gene confers transferable colistin resistance. mcr-1-positive Enterobacteriaceae (MCRPE) have attracted substantial medical, media, and political attention; however, so far studies have not addressed their clinical impact. Herein, we report the prevalence of MCRPE in human infections and carriage, clinical associations of mcr-1-positive Escherichia coli (MCRPEC) infection, and risk factors for MCRPEC carriage. METHODS: We undertook this study at two hospitals in Zhejiang and Guangdong, China...
January 27, 2017: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/28135233/use-of-colistin-in-a-neonatal-intensive-care-unit-a-cohort-study-of-65-patients
#18
Eren Çağan, Evrim Kıray Baş, Hüseyin Selim Asker
BACKGROUND The emergence of infections related to multidrug-resistant Gram-negative bacilli (MDR-GNB) reintroduced the use of colistin, an antibiotic that was previously abandoned due to adverse effects. However, because of its limited use in neonatal intensive care units, there is very little data about the effectiveness and safety of colistin in children and newborns. In this study, which will be the largest case study in the literature, we aimed to evaluate the effectiveness and safety of colistin in full-term and preterm newborns...
January 30, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28134988/the-essential-oil-from-the-fruits-of-the-brazilian-spice-xylopia-sericea-a-st-hil-presents-expressive-in-vitro-antibacterial-and-antioxidant-activity
#19
Renata de F Mendes, Nícolas de C C Pinto, Josiane M da Silva, Jucélia B da Silva, Raquel C Dos S Hermisdorf, Rodrigo L Fabri, Luciana M Chedier, Elita Scio
OBJECTIVES: The aims of this study were to investigate the chemical composition and the antioxidant activity and antibacterial activity of the essential oil of Xylopia sericea fruits (OXS). The fruits of this species are popularly used for medicinal purposes, and as a condiment in food preparation. METHODS: The chemical composition of OXS was analysed by GC/MS. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging, β-carotene/linoleic acid bleaching and phosphomolybdenum and thiobarbituric acid-reactive substance (TBARS) assays were used to evaluate the antioxidant activity...
March 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28131557/-profile-of-bacterial-resistance-in-pediatric-urinary-tract-infections-in-2014
#20
A Flammang, R Morello, M Vergnaud, J Brouard, P Eckart
In pediatric units, bacteria-producing extended-spectrum-betalactamase (ESBL) have an increasing prevalence among bacteria causing febrile urinary tract infections (UTIs). The purpose of this study was to evaluate the epidemiology of bacteria resistance patterns observed in UTIs, in order to assess the current antibiotic treatment protocols. This study is based upon a single-center retrospective chart review of the cytobacteriological urine cultures performed in UTIs between 1 January and 31 December 2014, in the medical pediatric unit of the Caen University Hospital...
January 25, 2017: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
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