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https://www.readbyqxmd.com/read/29351668/evaluation-of-the-rapid-carbapenem-inactivation-method-rcim-a-phenotypic-screening-test-for-carbapenemase-producing-enterobacteriaceae
#1
Madalina-Maria Muntean, Andrei-Alexandru Muntean, Lauraine Gauthier, Elodie Creton, Garance Cotellon, Mircea Ioan Popa, Rémy A Bonnin, Thierry Naas
Objectives: Fast and accurate diagnostic tests to identify carbapenemase-producing Enterobacteriaceae (CPE) are mandatory for proper antimicrobial therapy and implementing infection control measures. Here, we have developed a rapid Carbapenem Inactivation Method (rCIM) for CPE detection. Methods: The rCIM consists of the incubation of a potential carbapenemase producer with meropenem discs and use of the resulting supernatant to challenge a susceptible indicator strain...
January 16, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29349568/class-iii-bacteriocin-helveticin-m-causes-sublethal-damage-on-target-cells-through-impairment-of-cell-wall-and-membrane
#2
Zhilan Sun, Xiaomeng Wang, Xinxiao Zhang, Haihong Wu, Ye Zou, Pengpeng Li, Chong Sun, Weimin Xu, Fang Liu, Daoying Wang
Helveticin-M, a novel Class III bacteriocin produced by Lactobacillus crispatus exhibited an antimicrobial activity against Staphylococcus aureus, S. saprophyticus, and Enterobacter cloacae. To understand how Helveticin-M injured target cells, Helveticin-M was cloned and heterologously expressed in Escherichia coli. Subsequently, the cell wall organization and cell membrane integrity of target cells were determined. The mechanism of cellular damage differed according to bacterial species. Based on morphology analysis, Helveticin-M disrupted the cell wall of Gram-positive bacteria and disorganized the outer membrane of Gram-negative bacteria, therefore, altering surface structure...
January 18, 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29344811/metabolic-evolution-and-a-comparative-omics-analysis-of-corynebacterium-glutamicum-for-putrescine-production
#3
Zhen Li, Yu-Ping Shen, Xuan-Long Jiang, Li-Shen Feng, Jian-Zhong Liu
Putrescine is widely used in the industrial production of bioplastics, pharmaceuticals, agrochemicals, and surfactants. Because the highest titer of putrescine is much lower than that of its precursor L-ornithine reported in microorganisms to date, further work is needed to increase putrescine production in Corynebacterium glutamicum. We first compared 7 ornithine decarboxylase genes and found that the Enterobacter cloacae ornithine decarboxylase gene speC1 was most suitable for putrescine production in C. glutamicum...
January 17, 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29343795/recurrent-wheezing-in-neonatal-pneumonia-is-associated-with-combined-infection-with-respiratory-syncytial-virus-and-staphylococcus-aureus-or-klebsiella-pneumoniae
#4
Qin Zhong, Hui Feng, Qi Lu, Xu Liu, Qi Zhao, Yue Du, Xian-Hong Zhang, Jia-Rong Wang
Both viral and bacterial infections can be associated with wheezing episodes in children; however, information regarding combined infections with both viral and bacterial pathogens in full term neonates is limited. We sought to investigate the effects of viral-bacterial codetection on pneumonia severity and recurrent wheezing. A retrospective cohort study was conducted on neonates admitted to our hospital with pneumonia from 2009 to 2015. Of 606 total cases, 341 were diagnosed with RSV only, and 265 were diagnosed with both RSV and a potential bacterial pathogen...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29342994/-report-of-antimicrobial-resistance-surveillance-program-in-chinese-children-in-2016
#5
C Q Wang, A M Wang, H Yu, H M Xu, C M Jing, J K Deng, R Z Zhao, C Z Hua, Y H Chen, X J Chen, T Zhang, H Zhang, Y P Chen, J G Yang, A W Lin, S F Wang, Q Cao, X Wang, H L Deng, S C Cao, J H He, W Gao, S Z Han
Objective: To analyze the antimicrobial resistance profile in Chinese children. Methods: This was a prevalence survey. From January 1 through December 31, 2016, the isolates were collected from 10 tertiary children hospitals in China. Antimicrobial susceptibility testing was carried out by routine laboratory methods. The penicillin susceptibility of streptococcus pneumonia and Meropenem susceptibility of gram-negative bacteria were detected by E-test and disk diffusion method respectively. Antimicrobial susceptibility results were interpreted according to the criteria of Clinical and Laboratory Standards Institute (CLSI) Guideline 2016...
January 2, 2018: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/29326158/reciprocal-spatiotemporally-controlled-apoptosis-regulates-wolffian-duct-cloaca-fusion
#6
Masato Hoshi, Antoine Reginensi, Matthew S Joens, James A J Fitzpatrick, Helen McNeill, Sanjay Jain
The epithelial Wolffian duct (WD) inserts into the cloaca (primitive bladder) before metanephric kidney development, thereby establishing the initial plumbing for eventual joining of the ureters and bladder. Defects in this process cause common anomalies in the spectrum of congenital anomalies of the kidney and urinary tract (CAKUT). However, developmental, cellular, and molecular mechanisms of WD-cloaca fusion are poorly understood. Through systematic analysis of early WD tip development in mice, we discovered that a novel process of spatiotemporally regulated apoptosis in WD and cloaca was necessary for WD-cloaca fusion...
January 11, 2018: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29320823/first-blindness-cases-of-horses-infected-with-setaria-digitata-nematoda-filarioidea-in-the-republic-of-korea
#7
Jihun Shin, Kyu-Sung Ahn, Guk-Hyun Suh, Ha-Jung Kim, Hak-Sub Jeong, Byung-Su Kim, Eunsang Choi, Sung-Shik Shin
Ocular setariases of cattle were reported but those of equine hosts have never been reported in the Republic of Korea (Korea). We found motile worms in the aqueous humor of 15 horses (Equus spp.) from 12 localities in southern parts of Korea between January 2004 and November 2017. After the affected animals were properly restrained under sedation and local anesthesia, 10 ml disposable syringe with a 16-gauge needle was inserted into the anterior chamber of the affected eye to successfully remove the parasites...
December 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29320234/influence-of-soap-characteristics-and-food-service-facility-type-on-the-degree-of-bacterial-contamination-of-open-refillable-bulk-soaps
#8
Donald W Schaffner, Dane Jensen, Charles P Gerba, David Shumaker, James W Arbogast
Concern has been raised regarding the public health risks from refillable bulk-soap dispensers because they provide an environment for potentially pathogenic bacteria to grow. This study surveyed the microbial quality of open refillable bulk soap in four different food establishment types in three states. Two hundred ninety-six samples of bulk soap were collected from food service establishments in Arizona, New Jersey, and Ohio. Samples were tested for total heterotrophic viable bacteria, Pseudomonas, coliforms and Escherichia coli, and Salmonella...
January 10, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29319420/molecular-identification-and-phylogenetic-analysis-of-bothrops-insularis-bacterial-and-fungal-microbiota
#9
Lidiane Nunes Barbosa, Rui Seabra Ferreira, Priscila Luiza Mello, Hans Garcia Garces, Jéssica Luana Chechi, Tarsila Frachin, Luciana Curtolo De Barros, Sandra De Moraes Guimenes Bosco, Eduardo Bagagli, Ary Fernandes Júnior, Benedito Barraviera, Lucilene Delazari Dos Santos
Bothrops insularis, known as the golden lancehead snake, has its natural habitat restricted to Queimada Grande Island on the southern coast of Brazil. This culture-dependent study aimed to identify microorganisms obtained from the mouth, eyes, and cloaca of this species. Swabs from 20 snakes were collected for fungal and bacterial isolation. DNA was extracted from all samples, and identification was performed by amplifying the ITS1-5.8S-ITS2 regions and the 16S rDNA gene, respectively. All strains were identified and deposited in the GenBank nucleotide database...
January 10, 2018: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/29311089/molecular-characterization-of-imp-1-producing-enterobacter-cloacae-complex-isolates-in-tokyo
#10
Kotaro Aoki, Sohei Harada, Koji Yahara, Yoshikazu Ishii, Daisuke Motooka, Shota Nakamura, Yukihiro Akeda, Tetsuya Iida, Kazunori Tomono, Satoshi Iwata, Kyoji Moriya, Kazuhiro Tateda
Although KPC enzymes are most common among carbapenemases produced by Enterobacter cloacae complex globally, the epidemiology varies from one country to another. While previous studies have suggested that IMP enzymes are most common in Japan, detailed analysis has been scarce thus far. Here we carried out a molecular epidemiological study and plasmid analysis of IMP-1-producing E. cloacae complex isolates collected from 3 hospitals in central Tokyo using whole genome sequencing. Seventy-one isolates were classified into several STs and 49 isolates were identified as Enterobacter hormaechei ST78...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29311088/antibiotic-resistance-plasmids-cointegrated-into-a-megaplasmid-harbouring-the-blaoxa-427-carbapenemase-gene
#11
Stefanie Desmet, Suruchi Nepal, Jan Maarten van Dijl, Marc Van Ranst, Monika A Chlebowicz, John W Rossen, Jeroen K J Van Houdt, Piet Maes, Katrien Lagrou, Erik Bathoorn
OXA-427 is a new class-D carbapenemase encountered in different species of Enterobacteriaceae in a Belgian hospital. To study the dispersal of this gene, we describe the comparative analysis of two plasmids containing the blaOXA-427 gene isolated from a Klebsiella pneumoniae and an Enterobacter cloacae complex strain. The two IncA/C2 plasmids containing blaOXA-427 share the same backbone, however, in the K. pneumoniae this plasmid is cointegrated into an IncFIB plasmid forming a 321 kb megaplasmid with multiple multi-resistance regions...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29306150/ultrasonographic-assessment-of-the-male-koala-phascolarctos-cinereus-reproductive-tract
#12
Rebecca Larkin, Chiara Palmieri, Motoharu Oishi, Lyndal Hulse, Stephen D Johnston
Studies documenting the application of ultrasonography to depict normal and pathological changes in koalas (Phascolarctos cinereus), especially in the male, are scarce. Sixty-two wild koalas were used in this study to define ultrasonographic protocols and features for the assessment of the male koala reproductive tract. Testis, epididymis and spermatic cord were examined using a hockey stick transducer. The normal koala testis showed a homogeneous echogenicity and an obvious hyper-echoic band corresponding to the tunica albuginea...
December 27, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29304717/analysis-of-the-spectrum-and-antibiotic-resistance-of-uropathogens-in-outpatients-at-a-tertiary-hospital
#13
Bo Yang, Fujia Yang, Shanmei Wang, Qian Wang, Zhirui Liu, Wei Feng, Fengjun Sun, Peiyuan Xia
The objective of this study was to analyse the distribution and antimicrobial resistance of bacterial uropathogens isolated from outpatients at Henan Provincial People's Hospital. A total of 1419 samples from 823 newly diagnosed and 596 recurrent UTI outpatients culture positive. Escherichia coli was the most common uropathogen. Compared with the recurrent group, the newly diagnosed group had a higher isolation rate of E. coli and Enterobacter cloacae but a lower isolation rate of Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter spp...
January 5, 2018: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29298861/hypersensitive-assessment-of-aryl-hydrocarbon-receptor-transcriptional-activity-using-a-novel-truncated-cyp1a-promoter-in-zebrafish
#14
Juan-Juan Luo, Dong-Sheng Su, Shao-Lin Xie, Yi Liu, Pei Liu, Xiao-Jun Yang, De-Sheng Pei
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent organic pollutant (POP), an unintentional byproduct of various industrial processes, and a human carcinogen. The expression of the cytochrome P450 1A (cyp1a) gene is upregulated in the presence of TCDD through activating the aryl hydrocarbon receptor pathway in a dose-dependent manner. Several essential response elements, including the 8 potential xenobiotic response elements in the cyp1a promoter region, have been identified to be the main functional parts for the response to TCDD...
January 3, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29298099/propidium-monoazide-quantitative-real-time-polymerase-chain-reaction-for-enumeration-of-some-viable-but-nonculturable-foodborne-bacteria-in-meat-and-meat-products
#15
Norhan Khairy Abd El-Aziz, Yasmine Hasanine Tartor, Ahlam Abd El-Aziz Gharib, Ahmed Mohamed Ammar
Foodborne infections due to bacterial pathogens are increasing worldwide. Given the surreptitious nature of viable but nonculturable (VBNC) bacteria, they largely remain a threat to public health and food safety due to their non-detectability through conventional plate count techniques. Hence, species-specific quantitative real-time polymerase chain reaction (PCR) (qPCR) alone and combined with the use of propidium monoazide (PMA) was used along with the plate count method to quantify VBNC Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, and Enterobacteriaceae in fresh and processed meat samples...
January 3, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29286016/granulated-sugar-for-adjuvant-treatment-of-surgical-wound-infection-due-to-multi-drug-resistant-pathogens-in-a-child-with-sarcoma-a-case-report-and-literature-review
#16
Aldo Naselli, Laura Accame, Piero Buffa, Anna Loy, Roberto Bandettini, Alberto Garaventa, Ornella Della Casa Alberighi, Elio Castagnola
The use of sugar for treating wounds which are difficult to heal and positive to resistant pathogens has already been documented. The authors describe the successful treatment by direct instillation of granular sugar in the antibiotic-resistant infected surgical site wound of a child with sarcoma. Sugar instillation in the extended spectrum beta-lactamase (ESBL)-producing Enterobacter cloacae positive wound, in addition to systemic treatment with meropenem and levofloxacin, allowed culture negativization in six days and complete wound healing in 30 days...
December 1, 2017: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/29279516/spontaneous-development-of-acute-obstructive-suppurative-pancreatic-ductitis-associated-with-pancreatic-carcinoma-a-first-case-report
#17
Kunio Iwatsuka, Hiroshi Nakagawara, Masahiro Ogawa, Takuji Gotoda, Shigeoki Hayashi, Noriko Kinukawa, Akihiro Hemmi, Kenji Yamao, Akio Yanagisawa, Mitsuhiko Moriyama
A 68-year-old man with a history of diabetes mellitus was admitted to our hospital with a diagnosis of acute pancreatitis. Abdominal computed tomography revealed a suspicious tumor in the body of the pancreas, along with a dilated main pancreatic duct and edema of the pancreatic tail. Endoscopic retrograde pancreatography was performed after treating the patient's pancreatitis. When a cannula tip was advanced beyond the stenosis, deep into the distal pancreatic duct, thick white pus was evacuated. A bacteriological examination of the aspirated pancreatic juice revealed Enterobacter cloacae, and a cytological examination revealed adenocarcinoma...
December 27, 2017: Internal Medicine
https://www.readbyqxmd.com/read/29274468/molecular-characterization-of-carbapenem-resistant-enterobacter-cloacae-complex-in-colombia-blakpc-and-the-changing-landscape
#18
Elsa De La Cadena, Adriana Correa, Juan Sebastián Muñoz, Laura J Rojas, Cristhian Hernández-Gómez, Christian Pallares, Federico Perez, Robert A Bonomo, Maria V Villegas
OBJECTIVES: To examine the population structure of representative carbapenem resistant Enterobacter cloacae complex (CR-Ecl) isolates from 8 different Colombian regions and to characterize their asociated β-lactamases. METHODS: Twenty-eight CR-Ecl isolates collected in Colombia between 2009 and 2013 through the Colombian Nosocomial Network were included. Antimicrobial susceptibility testing was performed by broth microdilution. Molecular detection of carbapenemases and extended-spectrum β-lactamases (ESBL), and the presence of Tn4401 was evaluated by PCR and sequencing...
December 20, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29258559/immune-related-redox-metabolism-of-embryonic-cells-of-the-tick-rhipicephalus-microplus-bme26-in-response-to-infection-with-anaplasma-marginale
#19
Sandra Patricia Kalil, Rafael Diego da Rosa, Janaína Capelli-Peixoto, Paula Cristiane Pohl, Pedro Lagerblad de Oliveira, Andrea Cristina Fogaça, Sirlei Daffre
BACKGROUND: It is well known that reactive oxygen species (ROS) and reactive nitrogen species (RNS) are involved in the control of pathogens and microbiota in insects. However, the knowledge of the role of ROS and RNS in tick-pathogen and tick-microbiota interactions is limited. Here, we evaluated the immune-related redox metabolism of the embryonic cell line BME26 from the cattle tick Rhipicephalus microplus in response to Anaplasma marginale infection. METHODS: A high-throughput qPCR approach was used to determine the expression profile of 16 genes encoding proteins involved in either production or detoxification of ROS and RNS in response to different microbial challenges...
December 19, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29248504/rapid-emergence-of-colistin-resistance-and-its-impact-on-mortality-in-healthcare-associated-infections
#20
Mehtap Aydin, Önder Ergönül, Alpay Azap, Hüseyin Bilgin, Güle Aydın, Sema Alp Çavuş, Yusuf Ziya Demiroğlu, Hikmet Eda Çalışkan, Osman Memikoğlu, Şirin Menekşe, Şafak Kaya, Nazlım Aktuğ Demir, İlkay Karaoğlan, Seniha Başaran, Çiğdem Hatipoğlu, Şebnem Erdinç, Emel Yılmaz, Ayhanım Tümtürk, Yasemin Tezer, Hamiyet Demirkaya, Şule Eren Çakar, Şiran Keske, SudaTekin, Cem Yardımcı, Çağla Karakoç, Pınar Ergen, Özlem Azap, Lütfiye Mülazımoğlu, Onur Ural, Füsun Can, Halis Akalın
We describe the emergence of resistance and predictors of fatality for 1556 cases of healthcare-associated Gram-negative bloodstream infections in 2014 and 2015. The colistin resistance rate in Klebsiella pneumoniae was 16.1%, compared with 6% in 2013. 660 (42.4%) cases were fatal. The highest fatality rate was among patients with Acinetobacter baumannii bacteremia (58%), followed by Pseudomonas aeruginosa (45%), Klebsiella pneumoniae (41%), Enterobacter cloacae (32%), and Escherichia coli (28%). In multivariate analysis, MIC for carbapenem (OR:1...
December 14, 2017: Journal of Hospital Infection
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