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langendorff heart perfusion system

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https://www.readbyqxmd.com/read/29310144/kolaviron-and-garcinia-kola-seed-extract-protect-against-ischaemia-reperfusion-injury-on-isolated-rat-heart
#1
Ademola Adetokunbo Oyagbemi, Dirk Bester, Johan Esterhuyse, Ebenezer Olatunde Farombi
BACKGROUND: The incidence of cardiovascular diseases and its associated complications have increased greatly in the past three decades. The purpose of this study was to evaluate the acute cardioprotective effects of Garcinia kola (GK) seed extract and Kolaviron (KV) and determine mechanisms of action involving RISK signalling pathways. METHODS: Male Wistar rats were used in this study. Hearts were excised and mounted on the Langendorff perfusion system. The control, group 1 was perfused with dimethyl sulfoxide (DMSO), group II with KV and group III with GK respectively...
January 8, 2018: Drug Research
https://www.readbyqxmd.com/read/29306618/milrinone-induced-postconditioning-requires-activation-of-mitochondrial-ca2-sensitive-potassium-mbkca-channels
#2
Friederike Behmenburg, Lara Trefz, Marianne Dorsch, Martin Ströthoff, Alexander Mathes, Annika Raupach, André Heinen, Markus W Hollmann, Marc M Berger, Ragnar Huhn
OBJECTIVES: Cardioprotection by postconditioning requires activation of mitochondrial large-conductance Ca2+-sensitive potassium (mBKCa) channels. The involvement of these channels in milrinone-induced postconditioning is unknown. The authors determined whether cardioprotection by milrinone-induced postconditioning involves activation of mBKCa channels in the rat heart in vitro. DESIGN: Randomized, prospective, blinded laboratory investigation. SETTING: Experimental laboratory...
November 29, 2017: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/29274077/increasing-t-type-calcium-channel-activity-by-%C3%AE-adrenergic-stimulation-contributes-to-%C3%AE-adrenergic-regulation-of-heart-rates
#3
Yingxin Li, Xiaoxiao Zhang, Chen Zhang, Xiaoying Zhang, Ying Li, Zhao Qi, Christopher Szeto, Mingxin Tang, Yizhi Peng, Jeffery D Molkentin, Steven R Houser, Mingxing Xie, Xiongwen Chen
OBJECTIVE: Cav3.1 (α1G ) T-type Ca2+ channel (TTCC) is expressed in mouse sinoatrial node cells (SANCs) and atrioventricular nodal (AVN) cells and contributes to heart rate genesis and AV conduction. However, its role in heart rate (HR) regulation and AV conduction acceleration by the β-adrenergic system (SAS) is unclear, which is the topic of this study. METHODS AND RESULTS: L- (ICa-L ) and T-type (ICa-T ) Ca2+ currents were recorded in SANCs from Cav3.1 transgenic (TG) and knockout (KO), and control mice...
December 23, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/29273867/a-new-multi-mode-perfusion-system-for-ex-vivo-heart-perfusion-study
#4
Liming Xin, Bryan Gellner, Roberto Vanin Pinto Ribeiro, Giulia Maria Ruggeri, David Banner, Massimiliano Meineri, Vivek Rao, Jean Zu, Mitesh V Badiwala
Ex vivo heart perfusion has been shown to be an effective means of facilitating the resuscitation and assessment of donor hearts for cardiac transplantation. Over the last ten years however, only a few ex vivo perfusion systems have been developed for this application. While results have been promising, a system capable of facilitating multiple perfusion strategies on the same platform has not yet been realized. In this paper, the design, development and testing of a novel and modular ex vivo perfusion system is described...
December 23, 2017: Journal of Medical Systems
https://www.readbyqxmd.com/read/29160855/effects-of-red-palm-oil-on-myocardial-antioxidant-enzymes-nitric-oxide-synthase-and-heart-function-in-spontaneously-hypertensive-rats
#5
Emma Katengua-Thamahane, Barbara Szeiffova Bacova, Iveta Bernatova, Matus Sykora, Vladimir Knezl, Jacques Van Rooyen, Narcis Tribulova
The purpose of this study was to investigate the effect of antioxidants rich red palm oil (RPO) supplementation on cardiac oxidative stress known as crucial factor deteriorating heart function in hypertension. 3-month-old, male spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY) were fed standard rat chow without or with RPO (0.2 mL/day/5 weeks). General characteristic of rats were registered. Left ventricular tissue (LV) was used to determine expression of superoxide dismutases (SOD1, SOD2) and glutathione peroxidases (Gpx) as well as activity of nitric oxide synthase (NOS)...
November 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29159980/role-of-pi3k-in-myocardial-ischaemic-preconditioning-mapping-pro-survival-cascades-at-the-trigger-phase-and-at-reperfusion
#6
Xavier Rossello, Jaime A Riquelme, Sean M Davidson, Derek M Yellon
The Reperfusion Injury Salvage Kinase (RISK) pathway is considered the main pro-survival kinase cascade mediating the ischaemic preconditioning (IPC) cardioprotective effect. To assess the role of PI3K-Akt, its negative regulator PTEN and other pro-survival proteins such as ERK and STAT3 in the context of IPC, C57BL/6 mouse hearts were retrogradely perfused in a Langendorff system and subjected to 4 cycles of 5 min. ischaemia and 5 min. reperfusion prior to 35 min. of global ischaemia and 120 min. of reperfusion...
November 20, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29155786/isolation-and-cultivation-of-adult-rat-cardiomyocytes
#7
Franziska Nippert, Rolf Schreckenberg, Klaus-Dieter Schlüter
In an intact heart, adjacent cells influence adult cardiomyocytes. With the method of isolation and cultivation of adult cardiomyocytes, a precise investigation of the behavior of these cells under specific treatments and environments is possible. This manuscript presents a protocol for successful isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). The rat is sacrificed by cervical dislocation under deep anesthesia. Then, the heart is extracted and the aorta is uncovered. Subsequently, perfusion on the Langendorff perfusion system with calcium depletion and collagenase treatment is performed...
October 19, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29095792/important-role-of-endothelial-caveolin-1-in-the-protective-role-of-edh-against-no-mediated-nitrative-stress-in-microcirculation-in-mice
#8
Hiroki Saito, Shigeo Godo, Saori Sato, Akiyo Ito, Yosuke Ikumi, Shuhei Tanaka, Tomoaki Ida, Shigemoto Fujii, Takaaki Akaike, Hiroaki Shimokawa
AIMS: Nitric oxide (NO) and endothelium-dependent hyperpolarization (EDH) play important roles in maintaining cardiovascular homeostasis. We have previously demonstrated that endothelial NO synthase (eNOS) plays diverse roles depending on vessel size, as a NO generating system in conduit arteries and an EDH-mediated system in resistance arteries, for which caveolin-1(Cav-1) is involved. However, the physiological role of endothelial Cav-1 in microvessels remains to be elucidated. METHODS AND RESULTS: We newly generated endothelium-specific Cav-1-knockout (eCav-1-KO) mice...
October 27, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/29073179/restitution-characteristics-of-his-bundle-and-working-myocardium-in-isolated-rabbit-hearts
#9
Shangwei Huang, Liqun Wu, Jian Huang, Nuttanont Panitchob, Nan Hu, Ravi Ranjan, Derek J Dosdall
The Purkinje system (PS) and the His bundle have been recently implicated as an important driver of the rapid activation rate after 1-2 minutes of ventricular fibrillation (VF). It is unknown whether activations during VF propagate through the His-Purkinje system to other portions of the the working myocardium (WM). Little is known about restitution characteristic differences between the His bundle and working myocardium at short cycle lengths. In this study, rabbit hearts (n = 9) were isolated, Langendorff-perfused, and electromechanically uncoupled with blebbistatin (10 μM)...
2017: PloS One
https://www.readbyqxmd.com/read/28955390/remote-ischemic-conditioning-by-effluent-collected-from-a-novel-isolated-hindlimb-model-reduces-infarct-size-in-an-isolated-heart-model
#10
Young Jin Youn, Byung-Su Yoo, Jung-Woo Son, Jun-Won Lee, Min-Soo Ahn, Sung Gyun Ahn, Jang-Young Kim, Seung-Hwan Lee, Junghan Yoon, Young Woo Eom, Ji-Eun Oh, Seong-Kyung Choi
BACKGROUND AND OBJECTIVES: Experimental protocols for remote ischemic conditioning (RIC) utilize models in which a tourniquet is placed around the hindlimb or effluent is collected from an isolated heart. In analyzing the humoral factors that act as signal transducers in these models, sampled blood can be influenced by systemic responses, while the effluent from an isolated heart might differ from that of the hindlimb. Thus, we designed a new isolated hindlimb model for RIC and tested whether the effluent from this model could affect ischemia/reperfusion (IR) injury and if the reperfusion injury salvage kinase (RISK) and survivor activating factor enhancement (SAFE) pathways are involved in RIC...
September 2017: Korean Circulation Journal
https://www.readbyqxmd.com/read/28920042/mitochondrial-atp-sensitive-potassium-channels-play-a-role-in-reducing-both-myocardial-infarction-and-reperfusion-arrhythmia-in-remote-ischemic-preconditioned-hearts
#11
Young-Ho Jang, June-Hong Kim, Yong-Cheol Lee
BACKGROUND: Mitochondrial ATP-sensitive potassium (mKATP) channels play a role in reperfusion arrhythmias (RAs) in ischemia-reperfusion (I/R) injury. Evidence suggests that remote ischemic preconditioning (RIPC) reduces RAs, however not much is known on the mechanistic role of mKATP in RIPC. We evaluated whether mKATP channels are associated with reducing arrhythmia and infarct size in RIPC. METHODS: Isolated rat hearts received 30 minutes of regional ischemia followed by 2 hours of reperfusion through the Langendorff perfusion system...
February 2017: Anesthesiology and Pain Medicine
https://www.readbyqxmd.com/read/28893911/molecular-and-functional-characterization-of-the-endothelial-atp-sensitive-potassium-channel
#12
Qadeer Aziz, Yiwen Li, Naomi Anderson, Leona Ojake, Elena Tsisanova, Andrew Tinker
ATP-sensitive potassium (KATP) channels are widely expressed in the cardiovascular system, where they regulate a range of biological activities by linking cellular metabolism with membrane excitability. KATP channels in vascular smooth muscle have a well-defined role in regulating vascular tone. KATP channels are also thought to be expressed in vascular endothelial cells, but their presence and function in this context are less clear. As a result, we aimed to investigate the molecular composition and physiological role of endothelial KATP channels...
October 27, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28877068/mc225-a-novel-probe-for-p-glycoprotein-pet-imaging-at-the-blood-brain-barrier-in-vitro-cardiovascular-safety-evaluation
#13
Fabio Fusi, Miriam Durante, Beatrice Gorelli, Maria Grazia Perrone, Colabufo Nicola Antonio, Simona Saponara
The P-glycoprotein (P-gp) substrate MC225, at concentrations ≤ 10 nM, is a valuable radiotracer for positron emission tomography (PET) imaging of P-gp function in rats and mice.The aim of this study was to evaluate its potential toxic hazard towards the cardiovascular system through an in-depth analysis of its effects on rat aorta rings, on CaV1.2 channel current (ICa1.2) of A7r5 cells and on Langendorff perfused rat heart.In aortic rings, MC225 relaxed phenylephrine-induced contraction in a concentration-dependent and endothelium-independent manner, with an IC50 value of about 1 µM...
September 1, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28766171/cisplatin-and-cisplatin-analogues-perfusion-through-isolated-rat-heart-the-effects-of-acute-application-on-oxidative-stress-biomarkers
#14
Isidora M Stojic, Vladimir I Zivkovic, Ivan M Srejovic, Tamara R Nikolic, Nevena S Jeremic, Jovana N Jeremic, Dragan M Djuric, Nemanja Jovicic, Katarina G Radonjic, Zivadin D Bugarcic, Vladimir L J Jakovljevic, Slobodan S Novokmet
Drug-induced oxidative stress can occur in numerous tissues and organ systems (liver, kidney, ear, nervous system, and cardiovascular system). Cancer therapy with cisplatin is associated with side effects to which oxidative stress may contribute. We have compared the influences of cisplatin (reference compound) and its' analogues (dichloro(1,2-diaminocyclohexane)platinum(II) and chloro(2,2':6',2″-terpyridine)platinum(II)) in a model of isolated rat heart using the Langendorff technique. The production of oxidative stress biomarkers, antioxidant enzymes, myocardial damage, and expression of Bax, OH-1, and SODs were studied...
August 1, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28758595/vasodilator-effect-of-angiotensin-1-7-on-vascular-coronary-bed-of-rats-role-of-mas-ace-and-ace2
#15
Patrícia Lanza Moraes, Lucas Miranda Kangussu, Carlos Henrique Castro, Robson As Santos, Anderson José Ferreira, Alvair P Almeida
Angiotensin(Ang)-(1-7) is a biologically active member of the renin-angiotensin system that participates of the regulation of blood pressure. Although Ang-(1-7) is able to potentiate the vasodilator effect of bradykinin in coronary bed of rats, a direct vasodilator effect of Ang-(1-7) in this vascular bed has not been characterized. Thus, the aim of this study was to evaluate the mechanisms involved in the vasodilator effect of Ang-(1-7) in the vasculature of isolated rat hearts perfused according to the Langendorff technique at constant flow...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758414/modulation-of-n-methyl-d-aspartate-receptors-in-isolated-rat-heart
#16
Ivan Srejovic, Vladimir Zivkovic, Tamara Nikolic, Nevena Jeremic, Isidora Stojic, Jovana Jeremic, Dragan Djuric, Vladimir Jakovljevic
Considering the limited data on the role of NMDA-Rs in the cardiovascular system, the aim of the present study was to examine the effects of NMDA and DL-Hcy TLHC, alone and in combination with glycine, memantine, and ifenprodil, in the isolated rat heart. The hearts of Wistar albino rats were retrogradely perfused according to the Langendorff technique at a constant perfusion pressure. The experimental protocol for all experimental groups included the stabilization period, application of estimated substance for 5 min, followed by a washout period of 10 min...
November 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28689254/desensitization-of-the-human-5-ht4-receptor-in-isolated-atria-of-transgenic-mice
#17
Ulrich Gergs, Julia Fritsche, Stephanie Fabian, Josepha Christ, Joachim Neumann
In the human cardiovascular system, serotonin (5-HT) exerts positive inotropic and chronotropic effects mediated by 5-HT4 receptors. Moreover, 5-HT4 receptor stimulation can cause arrhythmias in the human heart. Response to 5-HT can fade due to desensitization of the receptor system and/or activation of phosphodiesterases. In this study, we investigated a potential desensitization of the human 5-HT4(a) receptor expressed in the mouse heart. Therefore, we have used atrial preparations of transgenic (TG) mice with cardiac myocyte-specific overexpression of the human 5-HT4(a) receptor and their non-transgenic littermates (WT)...
July 8, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28676988/posttraumatic-stress-disorder-disturbs-coronary-tone-and-its-regulatory-mechanisms
#18
Svetlana S Lazuko, Olga P Kuzhel, Lyudmila E Belyaeva, Eugenia B Manukhina, H Fred Downey, Olga B Tseilikman, Maria V Komelkova, Vadim E Tseilikman
Posttraumatic stress disorder (PTSD) is associated with myocardial injury, but changes in coronary regulatory mechanisms in PTSD have not been investigated. This study evaluated the effect of PTSD-inducing stress on coronary tone and its regulation by nitric oxide (NO) and voltage-gated K(+) channels. PTSD was induced by exposing rats to predator stress, 15 min daily for 10 days, followed by 14 stress-free days. Presence of PTSD was confirmed by the elevated plus-maze test. Coronary tone was evaluated from changes in coronary perfusion pressure of Langendorff isolated hearts...
July 4, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28671970/cardioprotective-effects-of-pkg-activation-by-soluble-gc-activator-bay-60-2770-in-ischemia-reperfusion-injured-rat-hearts
#19
Kyung Hye Lee, So-Ra Lee, Haneul Cho, Jong Shin Woo, Jung Hee Kang, Yun-Mi Jeong, Xian Wu Cheng, Woo-Shik Kim, Weon Kim
Soluble guanylate cyclase (sGC) has been suggested as a therapeutic target for cardiac ischemia-reperfusion (IR) injury. Until now, the molecular mechanism of BAY 60-2770, a sGC activator, in cardiac IR injury has not been assessed. To identify the cardioprotective effects of BAY 60-2770 in IR-injured rat hearts, IR injury was established by occlusion of LAD for 40 min and reperfusion for 7 days, and the effects of BAY 60-2770 on myocardial protection were assessed by echocardiography and TTC staining. 5 nM and 5 μM of BAY 60-2770 were perfused into isolated rat hearts in a Langendorff system...
2017: PloS One
https://www.readbyqxmd.com/read/28641963/modulation-of-the-heart-s-electrical-properties-by-the-anticonvulsant-drug-retigabine
#20
Lena Rubi, Michael Kovar, Eva Zebedin-Brandl, Xaver Koenig, Manuel Dominguez-Rodriguez, Hannes Todt, Helmut Kubista, Stefan Boehm, Karlheinz Hilber
Retigabine, currently used as antiepileptic drug, has a wide range of potential medical uses. Administration of the drug in patients can lead to QT interval prolongation in the electrocardiogram and to cardiac arrhythmias in rare cases. This suggests that the drug may perturb the electrical properties of the heart, and the underlying mechanisms were investigated here. Effects of retigabine on currents through human cardiac ion channels, heterologously expressed in tsA-201 cells, were studied in whole-cell patch-clamp experiments...
August 15, 2017: Toxicology and Applied Pharmacology
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