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langendorff heart perfusion system

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https://www.readbyqxmd.com/read/29732984/knockout-of-trpv1-exacerbates-left-ventricular-diastolic-dysfunction-induced-by-a-high-fat-diet-in-mice
#1
Beihua Zhong, Jack Rubinstein, Shuangtao Ma, Donna H Wang
BACKGROUND: Transient receptor potential vanilloid 1 (TRPV1) channels in sensory nerves have anti-oxidative properties and counteract obesity and diabetes that are associated with diastolic dysfunction with preserved ejection fraction. We tested the hypothesis that TRPV1 knockout exacerbates high-fat diet (HFD)-induced glucose intolerance and diastolic dysfunction. METHOD: Trpv1-/- and wild-type (WT) mice were fed chow diet or HFD for 20 weeks. Then, we performed the intraperitoneal glucose tolerance test, measured the heart function through transthoracic echocardiography and Langendorff heart perfusion system, analyzed cardiac histology, and measured the myocardial superoxide production and the expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidases...
May 3, 2018: Cardiovascular & Hematological Disorders Drug Targets
https://www.readbyqxmd.com/read/29581158/chymase-released-from-hypoxia-activated-cardiac-mast-cells-cleaves-human-apolipoproteina-i-at-tyr192-and-compromises-its-cardioprotective-activity
#2
Ilona Kareinen, Marc Baumann, Su Duy Nguyen, Katariina Maaninka, Andrey Anisimov, Minoru Tozuka, Matti Jauhiainen, Miriam Lee-Rueckert, Petri T Kovanen
Apolipoprotein A-I (apoA-I), the main structural and functional protein of HDL particles, is cardioprotective, but also highly sensitive to proteolytic cleavage. Here we investigated the effect of cardiac mast cell activation and ensuing chymase secretion on apoA-I degradation using isolated rat hearts in the Langendorff perfusion system. Cardiac mast cells were activated by injection of compound 48/80 into the coronary circulation or by low-flow myocardial ischemia, after which lipid-free apoA-I was injected, and collected in the coronary effluent for cleavage analysis...
March 26, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29553490/electrophysiological-assessment-of-murine-atria-with-high-resolution-optical-mapping
#3
Kensuke Ihara, Koji Sugiyama, Kentaro Takahashi, Masahiro Yamazoe, Tetsuo Sasano, Tetsushi Furukawa
Recent genome-wide association studies targeting atrial fibrillation (AF) have indicated a strong association between the genotype and electrophysiological phenotype in the atria. That encourages us to utilize a genetically-engineered mouse model to elucidate the mechanism of AF. However, it is difficult to evaluate the electrophysiological properties in murine atria due to their small size. This protocol describes the electrophysiological evaluation of atria using an optical mapping system with a high temporal and spatial resolution in Langendorff perfused murine hearts...
February 22, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29538014/bioengineering-cardiac-tissue-constructs-with-adult-rat-cardiomyocytes
#4
Ze-Wei Tao, Mohamed Mohamed, Jeffrey G Jacot, Ravi K Birla
Bioengineering cardiac tissue constructs with adult cardiomyocytes may help treat adult heart defects and injury. In this study, we fabricated cardiac tissue constructs by seeding adult rat cardiomyocytes on a fibrin gel matrix and analyzed the electromechanical properties of the formed cardiac tissue constructs. Adult rat cardiomyocytes were isolated with a collagenase type II buffer using an optimized Langendorff perfusion system. Cardiac tissue constructs were fabricated using either indirect plating with cardiomyocytes that were cultured for 1 week and dedifferentiated or with freshly isolated cardiomyocytes...
March 13, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/29528120/contractile-heterogeneity-in-ventricular-myocardium
#5
Wen Pan, Ziqi Yang, Jun Cheng, Cheng Qian, Yanggan Wang
The transmural heterogeneity of the contractility in ventricular muscle has not been well-studied. Here, we investigated the calcium transient and sarcomere contraction/relaxation in the endocardial (Endo) and epicardial (Epi) myocytes. Endo and Epi myocytes were isolated from C57/BL6 mice by Langendorff perfusion. Ca2+ transient and sarcomere contraction/relaxation were recorded simultaneously at different stimulation frequencies using a dual excitation fluorescence photomultiplier system. We found that the Endo myocytes have higher baseline diastolic calcium, significantly larger calcium transient and stronger sarcomere shortening than Epi myocytes...
March 12, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29502061/-establishment-of-an-ex-vivo-myocardial-ischemia-reperfusion-model-in-tree-shrews
#6
Tian-Rui Yang, Yun-Bo Miao, Rong-Ping Zhang, Jin-Wen Yu
OBJECTIVE: To establish an ex vivo model of myocardial ischemia reperfusion in tree shrews. METHODS: The Langendorff ex vivo heart perfusion system was used to establish the myocardial ischemia reperfusion model in tree shrews with different irrigation and reperfusion time settings. Alanine aminotransferase (ALT), aspartate transaminase (AST) and lactic dehydrogenase (LDH) levels were measured by enzyme-labeled immunosorbent assay, creatine kinase MB (CK-MB) was detected using immunosuppression method, and malondialdehyde was measured with thiobarbital staining method; the infarct size was measured using 2, 3, 5-triphenyltrazoliumchloride (TTC) method...
February 20, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/29462717/evaluating-the-impact-of-diabetes-and-diabetic-cardiomyopathy-rat-heart-on-the-outcome-of-ischemia-reperfusion-associated-oxidative-stress
#7
A Mahalakshmi, Gino A Kurian
Earlier literature underlines that oxidative stress plays a major role in the pathology of myocardial ischemia-reperfusion (I/R) injury, diabetic cardiomyopathy (DCM), diabetes mellitus (DM), fibrosis and hypertrophy which could adversely affect the normal cardiac function. However, the contributory role of oxidative stress in I/R pathology of heart with pre-existing abnormalities or diseases like DM and DCM remains to be explored. I/R injury was induced in normal (normal diet), DM (normal diet + streptozotocin: multiple low dose of 30 mg/kg) and DCM (high fat diet (40% fat) + streptozotocin: multiple low dose of 30 mg/kg) rat hearts using Langendorff isolated heart perfusion apparatus...
April 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29419573/important-role-of-endothelial-caveolin-1-in-the-protective-role-of-endothelium-dependent-hyperpolarization-against-nitric-oxide-mediated-nitrative-stress-in-microcirculation-in-mice
#8
Hiroki Saito, Shigeo Godo, Saori Sato, Akiyo Ito, Yosuke Ikumi, Shuhei Tanaka, Tomoaki Ida, Shigemoto Fujii, Takaaki Akaike, Hiroaki Shimokawa
AIMS: Nitric oxide (NO) and endothelium-dependent hyperpolarization (EDH) play important roles in maintaining cardiovascular homeostasis. We have previously demonstrated that endothelial NO synthase (eNOS) plays diverse roles depending on vessel size, as a NO generating system in conduit arteries and an EDH-mediated system in resistance arteries, for which caveolin-1 (Cav-1) is involved. However, the physiological role of endothelial Cav-1 in microvessels remains to be elucidated. METHODS AND RESULTS: We newly generated endothelium-specific Cav-1-knockout (eCav-1-KO) mice...
February 2018: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/29414428/survival-kinase-dependent-pathways-contribute-to-gender-difference-in-the-response-to-myocardial-ischemia-reperfusion-and-ischemic-post-conditioning
#9
Alejandro Ciocci Pardo, Sergio Scuri, Luisa F González Arbeláez, Claudia Caldiz, Juliana Fantinelli, Susana M Mosca
The response to ischemia/reperfusion and the effects of ischemic post-conditioning (IPC) are sex-dependent, but the mechanisms have not been clarified. Male (M) and female (F) rat hearts isolated and perfused using the Langendorff technique were subject to 30 min of global ischemia (GI) and 60 min reperfusion (R). In IPC hearts, three cycles of 30-sec GI/30-sec R were applied at the beginning of R. Infarct size and myocardial function were assessed. Superoxide production, antioxidant systems, and expressions of phosphorylated forms of serine/threonine kinase (Akt), glycogen synthase kinase 3β (GSK-3β), protein kinase C ε (PKCε), endothelial nitric oxide synthase (eNOS), and apoptosis were measured...
March 2018: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/29409011/cardiomyocyte-specific-deletion-of-sirt1-gene-sensitizes-myocardium-to-ischemia-and-reperfusion-injury
#10
Lin Wang, Nanhu Quan, Wanqing Sun, Xu Chen, Courtney Cates, Thomas Rousselle, Xinchun Zhou, Xuezhong Zhao, Ji Li
Aim: A longevity gene, Sirtuin 1 (SIRT1) and energy sensor AMP-activated protein kinase (AMPK) have common activators such as caloric restriction, oxidative stress and exercise. The objective of this study is to characterize the role of cardiomyocyte SIRT1 in age-related impaired ischemic AMPK activation and increased susceptibility to ischemic insults. Methods and Results: Mice were subjected to ligation of left anterior descending coronary artery for in vivo ischemic models...
February 2, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29310144/kolaviron-and-garcinia-kola-seed-extract-protect-against-ischaemia-reperfusion-injury-on-isolated-rat-heart
#11
Ademola Adetokunbo Oyagbemi, Dirk Bester, Johan Esterhuyse, Ebenezer Olatunde Farombi
BACKGROUND: The incidence of cardiovascular diseases and its associated complications have increased greatly in the past three decades. The purpose of this study was to evaluate the acute cardioprotective effects of Garcinia kola (GK) seed extract and Kolaviron (KV) and determine mechanisms of action involving RISK signalling pathways. METHODS: Male Wistar rats were used in this study. Hearts were excised and mounted on the Langendorff perfusion system. The control, group 1 was perfused with dimethyl sulfoxide (DMSO), group II with KV and group III with GK respectively...
January 8, 2018: Drug Research
https://www.readbyqxmd.com/read/29306618/milrinone-induced-postconditioning-requires-activation-of-mitochondrial-ca-2-sensitive-potassium-mbk-ca-channels
#12
Friederike Behmenburg, Lara Trefz, Marianne Dorsch, Martin Ströthoff, Alexander Mathes, Annika Raupach, André Heinen, Markus W Hollmann, Marc M Berger, Ragnar Huhn
OBJECTIVES: Cardioprotection by postconditioning requires activation of mitochondrial large-conductance Ca2+ -sensitive potassium (mBKCa ) channels. The involvement of these channels in milrinone-induced postconditioning is unknown. The authors determined whether cardioprotection by milrinone-induced postconditioning involves activation of mBKCa channels in the rat heart in vitro. DESIGN: Randomized, prospective, blinded laboratory investigation. SETTING: Experimental laboratory...
November 29, 2017: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/29274077/increasing-t-type-calcium-channel-activity-by-%C3%AE-adrenergic-stimulation-contributes-to-%C3%AE-adrenergic-regulation-of-heart-rates
#13
Yingxin Li, Xiaoxiao Zhang, Chen Zhang, Xiaoying Zhang, Ying Li, Zhao Qi, Christopher Szeto, Mingxin Tang, Yizhi Peng, Jeffery D Molkentin, Steven R Houser, Mingxing Xie, Xiongwen Chen
Cav3.1 T-type Ca2+ channel current (ICa-T ) contributes to heart rate genesis but is not known to contribute to heart rate regulation by the sympathetic/β-adrenergic system (SAS). We show that the loss of Cav3.1 makes the beating rates of the heart in vivo and perfused hearts ex vivo, as well as sinoatrial node cells, less sensitive to β-adrenergic stimulation; it also renders less conduction acceleration through the atrioventricular node by β-adrenergic stimulation. Increasing Cav3.1 in cardiomyocytes has the opposite effects...
April 1, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29273867/a-new-multi-mode-perfusion-system-for-ex-vivo-heart-perfusion-study
#14
Liming Xin, Bryan Gellner, Roberto Vanin Pinto Ribeiro, Giulia Maria Ruggeri, David Banner, Massimiliano Meineri, Vivek Rao, Jean Zu, Mitesh V Badiwala
Ex vivo heart perfusion has been shown to be an effective means of facilitating the resuscitation and assessment of donor hearts for cardiac transplantation. Over the last ten years however, only a few ex vivo perfusion systems have been developed for this application. While results have been promising, a system capable of facilitating multiple perfusion strategies on the same platform has not yet been realized. In this paper, the design, development and testing of a novel and modular ex vivo perfusion system is described...
December 23, 2017: Journal of Medical Systems
https://www.readbyqxmd.com/read/29160855/effects-of-red-palm-oil-on-myocardial-antioxidant-enzymes-nitric-oxide-synthase-and-heart-function-in-spontaneously-hypertensive-rats
#15
Emma Katengua-Thamahane, Barbara Szeiffova Bacova, Iveta Bernatova, Matus Sykora, Vladimir Knezl, Jacques Van Rooyen, Narcis Tribulova
The purpose of this study was to investigate the effect of antioxidants rich red palm oil (RPO) supplementation on cardiac oxidative stress known as crucial factor deteriorating heart function in hypertension. 3-month-old, male spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY) were fed standard rat chow without or with RPO (0.2 mL/day/5 weeks). General characteristic of rats were registered. Left ventricular tissue (LV) was used to determine expression of superoxide dismutases (SOD1, SOD2) and glutathione peroxidases (Gpx) as well as activity of nitric oxide synthase (NOS)...
November 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29159980/role-of-pi3k-in-myocardial-ischaemic-preconditioning-mapping-pro-survival-cascades-at-the-trigger-phase-and-at-reperfusion
#16
Xavier Rossello, Jaime A Riquelme, Sean M Davidson, Derek M Yellon
The Reperfusion Injury Salvage Kinase (RISK) pathway is considered the main pro-survival kinase cascade mediating the ischaemic preconditioning (IPC) cardioprotective effect. To assess the role of PI3K-Akt, its negative regulator PTEN and other pro-survival proteins such as ERK and STAT3 in the context of IPC, C57BL/6 mouse hearts were retrogradely perfused in a Langendorff system and subjected to 4 cycles of 5 min. ischaemia and 5 min. reperfusion prior to 35 min. of global ischaemia and 120 min. of reperfusion...
February 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29155786/isolation-and-cultivation-of-adult-rat-cardiomyocytes
#17
Franziska Nippert, Rolf Schreckenberg, Klaus-Dieter Schlüter
In an intact heart, adjacent cells influence adult cardiomyocytes. With the method of isolation and cultivation of adult cardiomyocytes, a precise investigation of the behavior of these cells under specific treatments and environments is possible. This manuscript presents a protocol for successful isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). The rat is sacrificed by cervical dislocation under deep anesthesia. Then, the heart is extracted and the aorta is uncovered. Subsequently, perfusion on the Langendorff perfusion system with calcium depletion and collagenase treatment is performed...
October 19, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29095792/important-role-of-endothelial-caveolin-1-in-the-protective-role-of-edh-against-no-mediated-nitrative-stress-in-microcirculation-in-mice
#18
Hiroki Saito, Shigeo Godo, Saori Sato, Akiyo Ito, Yosuke Ikumi, Shuhei Tanaka, Tomoaki Ida, Shigemoto Fujii, Takaaki Akaike, Hiroaki Shimokawa
AIMS: Nitric oxide (NO) and endothelium-dependent hyperpolarization (EDH) play important roles in maintaining cardiovascular homeostasis. We have previously demonstrated that endothelial NO synthase (eNOS) plays diverse roles depending on vessel size, as a NO generating system in conduit arteries and an EDH-mediated system in resistance arteries, for which caveolin-1(Cav-1) is involved. However, the physiological role of endothelial Cav-1 in microvessels remains to be elucidated. METHODS AND RESULTS: We newly generated endothelium-specific Cav-1-knockout (eCav-1-KO) mice...
October 27, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/29073179/restitution-characteristics-of-his-bundle-and-working-myocardium-in-isolated-rabbit-hearts
#19
Shangwei Huang, Liqun Wu, Jian Huang, Nuttanont Panitchob, Nan Hu, Ravi Ranjan, Derek J Dosdall
The Purkinje system (PS) and the His bundle have been recently implicated as an important driver of the rapid activation rate after 1-2 minutes of ventricular fibrillation (VF). It is unknown whether activations during VF propagate through the His-Purkinje system to other portions of the the working myocardium (WM). Little is known about restitution characteristic differences between the His bundle and working myocardium at short cycle lengths. In this study, rabbit hearts (n = 9) were isolated, Langendorff-perfused, and electromechanically uncoupled with blebbistatin (10 μM)...
2017: PloS One
https://www.readbyqxmd.com/read/28955390/remote-ischemic-conditioning-by-effluent-collected-from-a-novel-isolated-hindlimb-model-reduces-infarct-size-in-an-isolated-heart-model
#20
Young Jin Youn, Byung-Su Yoo, Jung-Woo Son, Jun-Won Lee, Min-Soo Ahn, Sung Gyun Ahn, Jang-Young Kim, Seung-Hwan Lee, Junghan Yoon, Young Woo Eom, Ji-Eun Oh, Seong-Kyung Choi
BACKGROUND AND OBJECTIVES: Experimental protocols for remote ischemic conditioning (RIC) utilize models in which a tourniquet is placed around the hindlimb or effluent is collected from an isolated heart. In analyzing the humoral factors that act as signal transducers in these models, sampled blood can be influenced by systemic responses, while the effluent from an isolated heart might differ from that of the hindlimb. Thus, we designed a new isolated hindlimb model for RIC and tested whether the effluent from this model could affect ischemia/reperfusion (IR) injury and if the reperfusion injury salvage kinase (RISK) and survivor activating factor enhancement (SAFE) pathways are involved in RIC...
September 2017: Korean Circulation Journal
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