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https://www.readbyqxmd.com/read/28641185/mechanistic-understanding-of-low-methylmercury-bioaccessibility-from-crayfish-procambarus-clarkii-muscle-tissue
#1
Qian Peng, Ben K Greenfield, Fei Dang, Yu Gong, Wenbo Bu, Huan Zhong
Recent research indicates that dietary exposure to mercury and other metals from crayfish consumption poses a human health concern, particularly in regions with high crayfish-consuming populations. To better understand consumption risk from methylmercury (MeHg), we quantified MeHg bioaccessibility in edible tail muscle of cooked red swamp crayfish (Procambarus clarkii, collected from seven cities in China), versus cooked fillet tissue of two finfish species: yellow croaker (Larimichthys polyactis) and snakehead (Channa argus)...
June 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28640973/production-of-red-flowered-oilseed-rape-via-the-ectopic-expression-of-orychophragmus-violaceus-ovpap2
#2
Wenqin Fu, Daozong Chen, Qi Pan, Fengfeng Li, Zhigang Zhao, Xianhong Ge, Zaiyun Li
Oilseed rape (Brassica napus L.), which has yellow flowers, is both an important oil crop and a traditional tourism resource in China, whereas the Orychophragmus violaceus, which has purple flowers, likely possesses a candidate gene or genes to alter the flower color of oilseed rape. A previously established B. napus line has a particular pair of O. violaceus chromosomes (M4) and exhibits slightly red petals. In this study, the transcriptomic analysis of M4, B. napus (H3), and O. violaceus with purple petals (OvP) and with white petals (OvW) revealed that most anthocyanin biosynthesis genes were up-regulated in both M4 and OvP...
June 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28640945/production-of-human-vitronectin-in-nicotiana-benthamiana-using-the-inpact-hyper-expression-platform
#3
Benjamin Dugdale, Maiko Kato, Pradeep Deo, Manuel Plan, Mark Harrison, Robyn Lloyd, Terry Walsh, Robert Harding, James Dale
Human vitronectin (hVN) is a glycoprotein that functions as a cell adhesion molecule and a regulator of coagulation in blood plasma and the extracellular matrix. In vitro, hVN is added to serum-free media in order to promote the adhesion of animal cells to tissue culture surfaces and the proliferation of undifferentiated stem cells. Here, we report the production of hVN in Nicotiana benthamiana using the inducible In Plant Activation (INPACT) hyper-expression platform. N. benthamiana plants were transformed with an INPACT expression cassette encoding hVN, and both the Tobacco yellow dwarf virus Rep/RepA activator and Tomato bushy stunt virus p19 gene under the transcriptional control of the ethanol-inducible AlcR:alcA gene switch...
June 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28636489/evaluation-of-countermeasures-for-red-light-running-by-traffic-simulator-based-surrogate-safety-measures
#4
Changju Lee, Jaehyun Jason So, Jiaqi Ma
OBJECTIVE: The conflicts among motorists entering a signalized intersection with the red light indication have become a national safety issue. Because of its sensitivity, efforts have been made to investigate the possible causes and effectiveness of countermeasures by the comparison sites and/or before-and-after studies. Nevertheless, these approaches are ineffective when comparison sites cannot be found, or crash data sets are not readily available or not reliable for statistical analysis...
June 21, 2017: Traffic Injury Prevention
https://www.readbyqxmd.com/read/28636152/dynamics-and-origin-of-cytokinins-involved-in-plant-manipulation-by-a-leaf-mining-insect
#5
Hui Zhang, Antoine Guiguet, Géraldine Dubreuil, Anna Kisiala, Peter Andreas, R J Neil Emery, Elisabeth Huguet, Mélanie Body, David Giron
Several herbivorous insects and plant-associated microorganisms control the phytohormonal balance, thus enabling them to successfully exploit the plant by inhibiting plant defences and withdrawing plant resources for their own benefit. The leaf-mining moth Phyllonorycter blancardella modifies the cytokinin (CK) profile of mined leaf-tissues, and the insect symbiotic bacteria Wolbachia is involved in the plant manipulation to the benefit of the insect host. To gain a deeper understanding into the possible origin and dynamics of CKs, we conducted an extensive characterization of CKs in larvae and in infected apple leaves...
June 21, 2017: Insect Science
https://www.readbyqxmd.com/read/28635588/genome-sequence-variation-in-the-constricta-strain-dramatically-alters-the-protein-interaction-and-localization-map-of-potato-yellow-dwarf-virus
#6
Chanyong Jang, Renyuan Wang, Joseph Wells, Fabian Leon, Mark Farman, John Hammond, Michael M Goodin
The genome sequence of the constricta strain of Potato yellow dwarf virus (CYDV) was determined to be 12 792 nt long and organized into seven ORFs with the gene order 3'-N-X-P-Y-M-G-L-5', which encodes the nucleocapsid, phospho, movement, matrix, glyco, and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. Cloned ORFs for each gene, except L, were used to construct a protein interaction and localization map (PILM) for this virus, which shares greater than 80 % amino acid similarity in all ORFs except X and P with the sanguinolenta strain of this species (SYDV)...
June 21, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28635346/effect-of-drug-related-cues-on-response-inhibition-through-abstinence-a-pilot-study-in-male-heroin-abstainers
#7
Bobo Su, Ling Yang, Grace Y Wang, Sha Wang, Shaomei Li, Hua Cao, Yan Zhang
BACKGROUND: Chronic heroin use can cause a deficit of inhibitory function, leading to a loss of control over drug use. Exposure to drug-related cues is considered as one of the contributing factors. However, it is unclear whether there are dynamic changes on the effect of drug-related cues on response inhibition following prolonged abstinence. OBJECTIVE: The present study investigated the effect of drug-related cues on response inhibition in heroin abstainers at different abstinent phases...
February 17, 2017: American Journal of Drug and Alcohol Abuse
https://www.readbyqxmd.com/read/28634442/mild-traumatic-brain-injury-evokes-pyramidal-neuron-axon-initial-segment-plasticity-and-diffuse-presynaptic-inhibitory-terminal-loss
#8
Michal Vascak, Jianli Sun, Matthew Baer, Kimberle M Jacobs, John T Povlishock
The axon initial segment (AIS) is the site of action potential (AP) initiation, thus a crucial regulator of neuronal activity. In excitatory pyramidal neurons, the high density of voltage-gated sodium channels (NaV1.6) at the distal AIS regulates AP initiation. A surrogate AIS marker, ankyrin-G (ankG) is a structural protein regulating neuronal functional via clustering voltage-gated ion channels. In neuronal circuits, changes in presynaptic input can alter postsynaptic output via AIS structural-functional plasticity...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28634431/the-aftermath-of-a-hepatic-artery-aneurysm-a-rare-etiology-of-biliary-obstruction
#9
Catherine Linzay, Abhishek Seth, Kunal Suryawala, Ankur Sheth, Moheb Boktor, John Bienvenu, Robby Rahim, Guillermo P Sangster, Paul A Jordan
BACKGROUND: Hepatic artery aneurysms (HAAs) constitute 14% to 20% of visceral artery aneurysms. Most HAAs are asymptomatic. Although rare, obstructive jaundice due to external bile duct compression or rupture of the HAA into the biliary tree with occlusion of the lumen from blood clots has been reported. CASE PRESENTATION: A 56-year-old white man presented to an outside hospital with symptoms of obstructive jaundice, including abdominal pain and yellowing of the skin...
2017: Clinical Medicine Insights. Gastroenterology
https://www.readbyqxmd.com/read/28634405/shedding-new-light-on-an-old-molecule-quinophthalone-displays-uncommon-n-to-o-excited-state-intramolecular-proton-transfer-esipt-between-photobases
#10
Gi Rim Han, Doyk Hwang, Seunghoon Lee, Jong Woo Lee, Eunhak Lim, Jiyoung Heo, Seong Keun Kim
Excited state dynamics of common yellow dye quinophthalone (QPH) was probed by femtosecond transient absorption spectroscopy. Multi-exponential decay of the excited state and significant change of rate constants upon deuterium substitution indicate that uncommon nitrogen-to-oxygen excited state intramolecular proton transfer (ESIPT) occurs. By performing density functional theory (DFT) and time-dependent density functional theory (TDDFT) calculations, we found that adiabatic surface crossing between the S1 and S2 states takes place in the photoreaction...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28633950/a-multi-scale-integrated-modeling-framework-to-measure-comprehensive-impact-of-coastal-reclamation-activities-in-yellow-river-estuary-china
#11
Yan Xu, Yanpeng Cai, Tao Sun, Qian Tan
In this paper, an improved multi-scale integrated modeling framework has been established to evaluate coastal reclamation intensity (CRI). About 7 indicators are considered, including ecological degradation intensity (EDI), hydrodynamic disturbance (IHD), engineering types, water quality, economic investment, population growth, and reclaimed land area. Meanwhile, an integrated framework enhanced methods in terms of (a) measuring intensity of ecological degradation process under multi-scale impact, (b) developing the indicator system of CRI, and discussing the driving forces and trends of coastal reclamation, (c) determining fuzzy preference relations of weight and calculating the specific value of CRI with the case study areas of Yellow River estuary from 2000 to 2015...
June 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28633947/occurrence-and-spatial-distribution-of-organophosphorus-flame-retardants-and-plasticizers-in-the-bohai-and-yellow-seas-china
#12
Mingyu Zhong, Jianhui Tang, Lijie Mi, Fei Li, Runmei Wang, Guopei Huang, Huifeng Wu
Seawater samples from 50 sites in the BS and YS were collected to investigate the spatial distribution of 7 OPs. Concentrations of the total OPs (ƩOPs) in the BS and YS ranged from 8.12ngL(-1) to 98.04ngL(-1) with a geometric mean (GM) of 23.70ngL(-1). Tris(1-chloro-2-propyl) phosphate (TCPP) was the dominant compound, followed by tris(2-chloroethyl) phosphate (TCEP) and triphenylphosphine oxide (TPPO). The ƩOPs together with the most commonly detected individual OPs (TCPP, TCEP, tris(1,3-dichloro-2-propyl) phosphate (TDCPP), tri-iso-butyl phosphate (TiBP), triphenyl phosphate (TPhP), and TPPO) tended to decrease from nearshore to offshore and from the surface to the bottom seawaters, indicating major land-based sources...
June 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28633855/nanocomposites-synthesis-characterization-and-its-application-to-removal-azo-dyes-using-ultrasonic-assisted-method-modeling-and-optimization
#13
Sima Porhemmat, Mehrorang Ghaedi, Ali Reza Rezvani, Mohammad Hossein Ahmadi Azqhandi, Ali Akbar Bazrafshan
S-doped and Cu- and Co-doped TiO2 was synthesized by a sol-gel method and characterized by FE-SEM, XRD, EDX and FTIR. The Co/Cu/S-TiO2 nanocomposite loaded on the activated carbon as new nanoadsorbent was used for simultaneous removal of methylene blue (MB) and sunset yellow (SY) from aqueous solution by ultrasonic-assisted adsorption method. In this work, central composite design (CCD) and adaptive neuro-fuzzy inference system (ANFIS) as a support tool for examining data and making prediction are used to recognize and predict the removal percentage in MB and SY dye solution of different concentrations...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28632115/flaviaesturariibacter-luteus-sp-nov-isolated-from-an-agricultural-floodplain-soil-and-emended-description-of-the-genus-flaviaesturariibacter
#14
Javier Pascual, Bärbel U Foesel, Alicia Geppert, Katharina J Huber, Jörg Overmann
A novel slow-growing bacterial strain designated as AW305T was isolated from an agricultural floodplain soil located in Mashare, Kavango region, Namibia. Cells stained Gram-negative, were non-motile, non-spore-forming, coccoid to rod-shaped and did not form a capsule. Colonies were yellow-pigmented, but flexirubin-type pigments were not detected. AW305T had an aerobic chemo-organoheterotrophic metabolism, using a narrow spectrum of carbon sources for growth, with preference for complex protein substrates, organic acids and amino acids...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28632113/paracandidimonas-soli-gen-nov-sp-nov-isolated-from-soil
#15
Peter Kämpfer, Hans-Jürgen Busse, John A McInroy, Stefanie P Glaeser
A slightly yellow-pigmented, Gram-stain-negative, rod-shaped bacterium, strain IMT-305T, was isolated from soil in Alabama, USA. Phylogenetic analysis based on the nearly full-length 16S rRNA gene sequence placed the strain in between the genera Pusillimonas, Parapusillimonas and Candidimonas with highest 16S rRNA gene sequence similarity to the type strain of Parapusillimonas granuli (97.5 %) and Candidimonas nitroreducens (97.4 %). The genomic G+C content of strain IMT-305T was 63.9 mol%. The main cellular fatty acids were C18:1ω7c, C17:0 cyclo, C16:0 and C16:1ω7c/iso-C15:0 2-OH (detected as summed feature 3)...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28631273/dermoscopic-features-of-ocular-and-extraocular-sebaceous-carcinomas
#16
Hanae Satomura, Dai Ogata, Eichi Arai, Tetsuya Tsuchida
Sebaceous carcinomas are rare but potentially fatal malignant adenocarcinomas with varying degrees of sebaceous differentiation. Several dermoscopic findings of extraocular sebaceous carcinomas have been reported; however, there are no previous reports of ocular sebaceous carcinomas detected using dermoscopy. We present a case of ocular sebaceous carcinomas showing specific findings from dermoscopy, and describe the common dermoscopic characteristics. In our cases and a review of cases in the literature, we noted that polymorphous vessels and yellowish areas were observed in all eight cases...
June 20, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/28630678/on-the-colour-of-wing-scales-in-butterflies-iridescence-and-preferred-orientation-of-single-gyroid-photonic-crystals
#17
Robert W Corkery, Eric C Tyrode
Lycaenid butterflies from the genera Callophrys, Cyanophrys and Thecla have evolved remarkable biophotonic gyroid nanostructures within their wing scales that have only recently been replicated by nanoscale additive manufacturing. These nanostructures selectively reflect parts of the visible spectrum to give their characteristic non-iridescent, matte-green appearance, despite a distinct blue-green-yellow iridescence predicted for individual crystals from theory. It has been hypothesized that the organism must achieve its uniform appearance by growing crystals with some restrictions on the possible distribution of orientations, yet preferential orientation observed in Callophrys rubi confirms that this distribution need not be uniform...
August 6, 2017: Interface Focus
https://www.readbyqxmd.com/read/28630625/quantification-of-antioxidant-phenolic-compounds-in-a-new-chrysanthemum-cultivar-by-high-performance-liquid-chromatography-with-diode-array-detection-and-electrospray-ionization-mass-spectrometry
#18
Ah-Reum Han, Hyo Young Kim, Yangkang So, Bomi Nam, Ik-Soo Lee, Joo-Won Nam, Yeong Deuk Jo, Sang Hoon Kim, Jin-Baek Kim, Si-Yong Kang, Chang Hyun Jin
The flowers of Chrysanthemum morifolium Ramat. have been used as an herbal tea and in traditional medicine, and the plant has been developed to produce horticultural cultivars of various colors and shapes. In this study, a new chrysanthemum cultivar with dark purple petals (C. morifolium cv. ARTI-Dark Chocolate; ADC) was developed by radiation-induced mutation breeding of its original cultivar with purple striped white petals (C. morifolium cv. Noble Wine, NW). The phenolic profile and antioxidant property of ADC were investigated and compared with NW and the commercially available medicinal herb, C...
2017: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/28630112/rapid-evolution-of-ovarian-biased-genes-in-the-yellow-fever-mosquito-aedes-aegypti
#19
Carrie A Whittle, Cassandra G Extavour
Males and females exhibit highly dimorphic phenotypes, particularly in their gonads, which is believed to be driven largely by differential gene expression. Typically, the protein sequences of genes upregulated in males, or male-biased genes, evolve rapidly as compared to female-biased and unbiased genes. To date, the specific study of gonad-biased genes remains uncommon in metazoans. Here, we identified and studied a total of 2,927, 2,013 and 4,449 coding-sequences (CDS) with ovary-biased, testis-biased and unbiased expression respectively in the yellow-fever mosquito Aedes aegypti The results showed that ovary-biased and unbiased CDS had higher nonsynonymous to synonymous substitution rates (dN/dS) and lower optimal codon usage (those codons that promote efficient translation) than testis-biased genes...
June 19, 2017: Genetics
https://www.readbyqxmd.com/read/28629501/mycobacterium-persicum-sp-nov-a-novel-species-closely-related-to-mycobacterium-kansasii-and-mycobacterium-gastri
#20
Abdolrazagh Hashemi Shahraki, Alberto Trovato, Mehdi Mirsaeidi, Emanuele Borroni, Parvin Heidarieh, Mohamad Hashemzadeh, Narges Shahbazi, Daniela M Cirillo, Enrico Tortoli
Four strains isolated in Iran from pulmonary specimens of unrelated patients are proposed as representative of a novel Mycobacterium species. Similarity, at the phenotypic level, with Mycobacterium kansasii is remarkable with the photochromogenic yellow pigmentation of the colonies being the salient feature. They differ, however, genotypically from this species and present unique sequences in 16S rRNA, hsp65 and rpoB genes. The average nucleotide identity and the genome-to-genome distance fully support the status of an independent species...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
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