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https://www.readbyqxmd.com/read/28346259/igg4-related-ophthalmic-disease-presenting-as-choroidal-and-orbital-lesions
#1
William S Gange, Stephen M Holland, Felipe De Alba
PURPOSE: To report a case of IgG4-related ophthalmic disease (IgG4-ROD) which presented as choroidal and orbital lesions. METHODS: Case report. RESULTS: A 64-year-old man presented with left eye photopsias and a history of IgG4-related perirenal fibrosis. Fundoscopic examination showed multiple bilateral yellow choroidal lesions, and optical coherence tomography showed multiple choroidal lesions. Magnetic resonance imaging of the orbits showed an enhancing lesion present circumferential to the optic nerve, but greater medially, abutting the posterior surface of the left globe...
March 24, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28345675/a-myb-transcription-factor-dcmyb6-is-involved-in-regulating-anthocyanin-biosynthesis-in-purple-carrot-taproots
#2
Zhi-Sheng Xu, Kai Feng, Feng Que, Feng Wang, Ai-Sheng Xiong
Carrots are widely grown and enjoyed around the world. Purple carrots accumulate rich anthocyanins in the taproots, while orange, yellow, and red carrots accumulate rich carotenoids in the taproots. Our previous studies indicated that variation in the activity of regulatory genes may be responsible for variations in anthocyanin production among various carrot cultivars. In this study, an R2R3-type MYB gene, designated as DcMYB6, was isolated from a purple carrot cultivar. In a phylogenetic analysis, DcMYB6 was grouped into an anthocyanin biosynthesis-related MYB clade...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28345657/multicolor-bioimaging-with-biosynthetic-zinc-nanoparticles-and-their-application-in-tumor-detection
#3
Yanjun Kang, Yi-Zhou Wu, Xianyun Hu, Xueqin Xu, Jie Sun, Rong Geng, Tongxing Huang, Xiaohang Liu, Yichen Ma, Ying Chen, Quan Wan, Xiaobang Qi, Gen Zhang, Xiaohui Zhao, Xin Zeng
Because they generate excellent images, nanoparticles (NPs), especially biosynthesized NPs, provide a new solution for tumor imaging. In this research, we unveil a novel type of biosynthesized NPs featuring multicolor fluorescence. These NPs exhibit little cytotoxicity to cells. The explored NPs, designated Zn-ZFP-GST NPs (Zinc NPs in abbreviation), are generated from leukemia cells treated with a Zn(2+) solution, while zinc-finger protein and glutathione S-transferase (GST) were also identified in the Zinc NPs...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28345652/synergistic-infection-of-bryv-and-pemv-2-increases-the-accumulations-of-both-bryv-and-bryv-derived-sirnas-in-nicotiana-benthamiana
#4
Cui-Ji Zhou, Xiao-Yan Zhang, Song-Yu Liu, Ying Wang, Da-Wei Li, Jia-Lin Yu, Cheng-Gui Han
Viral synergism is caused by co-infection of two unrelated viruses, leading to more severe symptoms or increased titres of one or both viruses. Synergistic infection of phloem-restricted poleroviruses and umbraviruses has destructive effects on crop plants. The mechanism underlying this synergy remains elusive. In our study, synergism was observed in co-infections of a polerovirus Brassica yellows virus (BrYV) and an umbravirus Pea enation mosaic virus 2 (PEMV 2) on Nicotiana benthamiana, which led to (1) increased titres of BrYV, (2) appearance of severe symptoms, (3) gain of mechanical transmission capacity of BrYV, (4) broader distribution of BrYV to non-vascular tissues...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28345615/transcriptome-analysis-of-molecular-mechanisms-responsible-for-light-stress-response-in-mythimna-separata-walker
#5
Yun Duan, ZhongJun Gong, RenHai Wu, Jin Miao, YueLi Jiang, Tong Li, XiaoBo Wu, YuQing Wu
Light is an important environmental signal for most insects. The Oriental Armyworm, Mythimna separata, is a serious pest of cereal crops worldwide, and is highly sensitive to light signals during its developmental and reproductive stages. However, molecular biological studies of its response to light stress are scarce, and related genomic information is not available. In this study, we sequenced and de novo assembled the transcriptomes of M. separata exposed to four different light conditions: dark, white light (WL), UV light (UVL) and yellow light (YL)...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28345196/flupyradifurone-effectively-manages-whitefly-bemisia-tabaci-med-hemiptera-aleyrodidae-and-tomato-yellow-leaf-curl-virus-tylcv-in-tomato
#6
Emmanouil Roditakis, Marianna Stavrakaki, Maria Grispou, Aikaterini Achimastou, Χavier Van Waetermeulen, Ralf Nauen, Anastasia Tsagkarakou
BACKGROUND: The cotton whitefly Bemisia tabaci (Gennadius) is among the most important pests of numerous crops and a vector of >100 plant viruses, causing significant crop losses worldwide. Managing the pest as well as inhibiting the transmission of major viruses such as Tomato yellow leaf curl virus (TYLCV) is of utmost importance for sustainable yields. The efficacy on both whiteflies and virus transmission of the novel systemic butenolide insecticide flupyradifurone, was investigated in this study...
March 27, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28344587/the-chloroplast-genome-of-passiflora-edulis-passifloraceae-assembled-from-long-sequence-reads-structural-organization-and-phylogenomic-studies-in-malpighiales
#7
Luiz A Cauz-Santos, Carla F Munhoz, Nathalie Rodde, Stephane Cauet, Anselmo A Santos, Helen A Penha, Marcelo C Dornelas, Alessandro M Varani, Giancarlo C X Oliveira, Hélène Bergès, Maria Lucia C Vieira
The family Passifloraceae consists of some 700 species classified in around 16 genera. Almost all its members belong to the genus Passiflora. In Brazil, the yellow passion fruit (Passiflora edulis) is of considerable economic importance, both for juice production and consumption as fresh fruit. The availability of chloroplast genomes (cp genomes) and their sequence comparisons has led to a better understanding of the evolutionary relationships within plant taxa. In this study, we obtained the complete nucleotide sequence of the P...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28344548/gsk-3%C3%AE-overexpression-alters-the-dendritic-spines-of-developmentally-generated-granule-neurons-in-the-mouse-hippocampal-dentate-gyrus
#8
Noemí Pallas-Bazarra, Asta Kastanauskaite, Jesús Avila, Javier DeFelipe, María Llorens-Martín
The dentate gyrus (DG) plays a crucial role in hippocampal-related memory. The most abundant cellular type in the DG, namely granule neurons, are developmentally generated around postnatal day P6 in mice. Moreover, a unique feature of the DG is the occurrence of adult hippocampal neurogenesis, a process that gives rise to newborn granule neurons throughout life. Adult-born and developmentally generated granule neurons share some maturational aspects but differ in others, such as in their positioning within the granule cell layer...
2017: Frontiers in Neuroanatomy
https://www.readbyqxmd.com/read/28343270/magnetic-field-discrimination-learning-and-memory-in-the-yellow-stingray-urobatis-jamaicensis
#9
Kyle C Newton, Stephen M Kajiura
Elasmobranch fishes (sharks, skates, and rays) have been hypothesized to use the geomagnetic field as a cue for orienting and navigating across a wide range of spatial scales. Magnetoreception has been demonstrated in many invertebrate and vertebrate taxa, including elasmobranchs, but this sensory modality and the cognitive abilities of cartilaginous fishes are poorly studied. Wild caught yellow stingrays, Urobatis jamaicensis (N = 8), underwent conditioning to associate a magnetic stimulus with a food reward in order to elicit foraging behaviors...
March 25, 2017: Animal Cognition
https://www.readbyqxmd.com/read/28343064/a-barley-homolog-of-yeast-atg6-is-involved-in-multiple-abiotic-stress-responses-and-stress-resistance-regulation
#10
Xiaowei Zeng, Zhanghui Zeng, Cuicui Liu, Weiyi Yuan, Ning Hou, Hongwu Bian, Muyuan Zhu, Ning Han
Autophagy is a highly conserved degradation pathway among eukaryote cells, which can recycle damaged or unwanted cell materials upon encountering stress conditions. As a key component of the Class III PI3K kinase complex, ATG6/Beclin-1 is essential for autophagosome formation. In this study, we isolated a putative HvATG6 gene in barley genome. The protein encoded by HvATG6 shares high sequence identity to ATG6 orthologs in rice and wheat, and has a typical autophagy-specific domain containing segments of repeated β-sheet-α-helix...
March 19, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28342998/field-evaluation-of-cucurbit-yellow-stunting-disorder-virus-transmission-by-bemisia-tabaci
#11
S J Castle, J P Palumbo, P Merten
Cucurbit yellow stunting disorder virus (CYSDV) is a whitefly-transmitted Crinivirus (Closteroviridae) that impacts melon production in many parts of the world including the USA. It has been responsible for melon crop loss in the southwestern U.S. since 2006 when it was first identified. Control strategies have revolved mainly around chemical control, but research to identify suitable products and approaches to implementing them has lagged. The current study investigated the performance of four systemic insecticides in the field while concurrently tracking CYSDV disease progression after controlled and natural whitefly inoculation of young melon plants...
March 22, 2017: Virus Research
https://www.readbyqxmd.com/read/28342034/molecular-characterization-of-a-new-begomovirus-infecting-mirabilis-jalapa-in-northern-india
#12
Aditya Kulshreshtha, Poonam Roshan, Dolly Sharma, Vipin Hallan
Begomoviruses are whitefly-transmitted single-stranded DNA viruses that are responsible for considerable economic losses. A begomovirus, alphasatellite and betasatellite were characterized in a Mirabilis jalapa plant exhibiting severe leaf curling and mottling symptoms. The complete viral genome shared highest sequence identity of 87% with pedilanthus leaf curl virus (AM712436), reported from Pakistan. Additionally, the viral genome was 84% identical to that of chilli leaf curl India virus (KX951415) and 83% identical to that of tobacco curly shoot virus (GU1999584), which were previously reported to infect M...
March 24, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28342033/sequencing-and-phylogenetic-analysis-of-tobacco-virus-2-a-polerovirus-from-nicotiana-tabacum
#13
Benguo Zhou, Fang Wang, Xuesong Zhang, Lina Zhang, Huafeng Lin
The complete genome sequence of a new virus, provisionally named tobacco virus 2 (TV2), was determined and identified from leaves of tobacco (Nicotiana tabacum) exhibiting leaf mosaic, yellowing, and deformity, in Anhui Province, China. The genome sequence of TV2 comprises 5,979 nucleotides, with 87% nucleotide sequence identity to potato leafroll virus (PLRV). Its genome organization is similar to that of PLRV, containing six open reading frames (ORFs) that potentially encode proteins with putative functions in cell-to-cell movement and suppression of RNA silencing...
March 24, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28342022/identifying-connexin-expression-and-determining-gap-junction-intercellular-communication-in-rainbow-trout-cells
#14
Joshua Hooper, Sarah J Poynter, Stephanie J DeWitte-Orr
Gap junctions are groups of membrane-bound channels that allow the passage of small molecules and ions between cells, permitting cell-cell communication. Because of their importance in cell homeostasis, gap junction presence and function were characterized in three commonly studied rainbow trout cell lines, namely RTgill-W1, RTgutGC, and RTG-2. Firstly, gap junction presence was determined by screening for gap junction protein alpha 7 and alpha 1 (GJA7 and GJA1) presence at the transcript level and GJA7 at the protein level...
March 24, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28341392/novel-synthesis-of-gold-nanoparticles-using-artemisia-vulgaris-l-leaf-extract-and-their-efficacy-of-larvicidal-activity-against-dengue-fever-vector-aedes-aegypti-l
#15
B Sundararajan, B D Ranjitha Kumari
The Aedes aegypti L. mosquito transmits dengue and yellow fever, which cause millions of death every year. Dengue is a mosquito-borne viral disease that has rapidly spread worldwide particularly in countries with tropical and subtropical climates areas. The present study denotes a simple and eco-friendly biosynthesis of gold nanoparticles using Artemisia vulgaris L. leaf extract as reducing agent. The synthesized gold nanoparticles were characterized by UV-Visible Spectroscopy, X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Dynamic Light Scattering (DLS), Zeta Potential (ZP), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray Spectroscopy (EDX)...
March 18, 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/28340778/aging-of-paper-ultra-fast-quantification-of-2-5-dihydroxyacetophenone-as-a-key-chromophore-in-cellulosics-by-reactive-paper-spray-mass-spectrometry
#16
A Schedl, T Zweckmair, F Kikul, U Henniges, T Rosenau, A Potthast
The detection of individual chromophores that contribute to the overall discoloration of paper or pulp ("yellowing") is a challenge because these substances are only present in extremely small amounts (ppm to ppb range). In this work, paper spray (PS) coupled with mass spectrometry was used to detect a low-concentrated cellulosic key-chromophore, 2,5-dihydroxyacetophenone (DHAP). Sensitivity was enhanced by derivatization with Girard's reagent T (GT). DHAP was successfully detected in historic paper samples and also was applied to model papers in order to investigate different factors that influence its generation: temperature, time, relative humidity, and the presence of iron ions, by means of a full factorial design...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28340769/development-of-a-nano-sio2-based-enzyme-linked-ligand-binding-assay-for-the-determination-of-ibuprofen-in-human-urine
#17
Qian-Long Wang, Jing Xie, Xing-De Li, Li-Sheng Ding, Jian Liang, Pei Luo, Lin-Sen Qing
The application domains of classic enzyme-linked ligand binding assay (ELBA) is relatively narrow due to the high cost and hardly available binding receptor. In here, we described for the first time the possibility of developing a new ELBA based on silica nanoparticles (nano-SiO2) to assess the ibuprofen in human urine. Nano-SiO2 with a large surface area was introduced as stationary phase to improve the analytical performance. In the experiment, a competitively binding procedure with human serum albumin (HSA) was performed between the ibuprofen presented in sample and horseradish peroxidase labeled ibuprofen (HRP-ibuprofen) subsequently added...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28340396/spectral-studies-on-anthracene-based-dual-sensor-for-hg-2-and-al-3-ions-with-two-distinct-output-modes-of-detection
#18
Navneet Kaur, Baljeet Kaur
A simple and easily synthesized colorimetric and fluorescent chemosensor 1, based on aromatic OH and imine moieties as a binding and anthracene as signaling unit, has been synthesized in a one-step procedure. The chemosensor 1 is developed as a dual chemosensor for detection of Hg(2+) and Al(3+) ions in CH3OH, which exhibited a color change from light yellow to dark yellow with Hg(2+) ions, enabling 1 a suitable "bare eye" indicator for Hg(2+) ions. On the other hand, fluorescent enhancement with blue shift along with brilliant cyan fluorescence was observed upon binding with Al(3+) ions...
March 15, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28340264/identification-of-19-loci-for-reproductive-traits-in-a-local-chinese-chicken-by-genome-wide-study
#19
Q C Fan, P F Wu, G J Dai, G X Zhang, T Zhang, Q Xue, H Q Shi, J Y Wang
Reproductive traits have long been studied and have an important influence on chicken breeding. To identify quantitative trait loci affecting reproductive traits, a genome-wide analysis of a Chinese chicken breed was performed to analyze age at first egg body weight at first egg, first egg weight, egg weight at the age of 300 days, egg weight at the age of 462 days, egg number at the age of 300 days, egg number between the ages of 300 and 462 days and egg number at the age of 462 days. Nineteen SNPs related to reproductive traits were presented (P < 1...
March 22, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28340221/nationwide-multicentre-kidney-biopsy-study-of-japanese-patients-with-type-2-diabetes
#20
Kengo Furuichi, Yukio Yuzawa, Miho Shimizu, Akinori Hara, Tadashi Toyama, Hiroshi Kitamura, Yoshiki Suzuki, Hiroshi Sato, Noriko Uesugi, Yoshifumi Ubara, Satoshi Hisano, Yoshihiko Ueda, Shinichi Nishi, Hitoshi Yokoyama, Tomoya Nishino, Kentaro Kohagura, Daisuke Ogawa, Koki Mise, Yugo Shibagaki, Kenjiro Kimura, Masakazu Haneda, Hirofumi Makino, Seiichi Matsuo, Takashi Wada
Background.: The clinical and pathologic manifestations of nephropathy due to type 2 diabetes are diverse, but large-scale pathologic studies with long-term observations are limited. Methods.: Kidney biopsies and clinical data of 600 patients with type 2 diabetes were collected retrospectively from 13 centres across Japan. Thirteen pathologic findings (nine glomerular lesions, two interstitial lesions and two vascular lesions) were clearly defined and scored. Results...
March 2, 2017: Nephrology, Dialysis, Transplantation
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