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https://www.readbyqxmd.com/read/28646143/a-comparison-between-sphere-and-rod-nanoparticles-regarding-their-in-vivo-biological-behavior-and-pharmacokinetics
#1
Yating Zhao, Yu Wang, Fu Ran, Yu Cui, Chang Liu, Qinfu Zhao, Yikun Gao, Da Wang, Siling Wang
In recent years, spherical nanoparticles has been studied extensively on biomedical applications including bioimaging and biosensing, diagnostics and theranostics, but the effect of the shape of nanoparticles has received little attention. In the present study, we designed three different shaped fluorescent mesoporous silica nanoparticles (MSNs), long rod nanoparticles (NLR), short rod nanoparticles (NSR), and spherical nanoparticles (NS) to systematically examine their behavior in vivo after oral administration...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28644494/feco-nanoparticles-embedded-carbon-nanofibers-as-robust-peroxidase-mimics-for-sensitive-colorimetric-detection-of-l-cysteine
#2
Zezhou Yang, Yun Zhu, Guangdi Nie, Meixuan Li, Ce Wang, Xiaofeng Lu
A simple and low cost detection of l-cysteine is essential in the fields of biosensors and medical diagnosis. In this study, we have developed a simple electrospinning, followed by calcination process to prepare FeCo nanoparticles embedded in carbon nanofibers (FeCo-CNFs) as an efficient peroxidase-like mimic for the detection of l-cysteine. FeCo nanoparticles are uniformly dispersed within CNFs, and their diameters are highly influenced by the calcination temperature. The calcination temperature also influences the peroxidase-like catalytic activity, and the maximum activity is achieved at a calcination temperature of 550 °C...
June 23, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28643851/fluorescent-competitive-aptasensor-for-detection-of-aflatoxin-b1
#3
Monali Mukherjee, Praveena Bhatt, Manonmani H K
Aflatoxin B1 (AFB1 ) is one of the most commonly found mycotoxins in food commodities, particularly cereals, oilseeds, spices and tree nuts. In the past decade, aptamers have come into limelight and emerged as a new biosensing element replacing antibodies in various detection formats. Herein we report a faster, more sensitive, high throughput method for the detection of AFB1 using AFB1 -specific aptamers. The assay format was based on a competitive reaction of the fluorescent tagged aptamer specific to AFB1 with the aflatoxin conjugate...
June 23, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/28641203/current-trends-in-electrochemical-sensing-and-biosensing-of-dna-methylation
#4
REVIEW
Ludmila Krejcova, Lukas Richtera, David Hynek, Jan Labuda, Vojtech Adam
DNA methylation plays an important role in physiological and pathological processes. Several genetic diseases and most malignancies tend to be associated with aberrant DNA methylation. Among other analytical methods, electrochemical approaches have been successfully employed for characterisation of DNA methylation patterns that are essential for the diagnosis and treatment of particular diseases. This article discusses current trends in the electrochemical sensing and biosensing of DNA methylation. Particularly, it provides an overview of applied electrode materials, electrode modifications and biorecognition elements applications with an emphasis on strategies that form the core DNA methylation detection approaches...
June 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28640313/expanding-applications-of-sers-through-versatile-nanomaterials-engineering
#5
M Fernanda Cardinal, Emma Vander Ende, Ryan A Hackler, Michael O McAnally, Peter C Stair, George C Schatz, Richard P Van Duyne
Surface-enhanced Raman scattering (SERS) spectroscopy has evolved into a cross-disciplinary analytical technique by unveiling relevant chemical, biological, material, and structural information. The focus of this review is on two critical properties for successfully expanding applications of SERS spectroscopy: quality of the plasmonic substrate and molecule localization to the substrate. In this review, we discuss recent work on quantifying SERS distance dependence, key factors for substrate characterization and performance evaluation, expansion of SERS applications through substrate development for UV plasmonics and short-distance capture strategies for optimizing analyte-surface structures...
June 22, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28636320/dna-loading-and-release-using-custom-tailored-poly-l-lysine-surfaces
#6
Amin Shakiba, Sagar L Patil, Oussama Zenasni, Monika E Schmitt, Preethi H Gunaratne, T Randall Lee
This manuscript describes the generation and study of surfaces modified with custom-crafted poly(L-lysine) (PLL) coatings for use in the loading and delivery of single-stranded DNA (ssDNA). The experimental strategy utilizes bidentate dithiol adsorbates to generate stably-bound azide-terminated self-assembled monolayers (SAMs) on gold possessing an oligo(ethylene glycol) (OEG) spacer. Consequent to the molecular assembly on gold, the azide termini are covalently attached to a maleimide linker moiety via a copper-catalyzed azide-alkyne "click" reaction...
June 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28636010/engineering-multi-functional-bacterial-outer-membrane-vesicles-as-modular-nanodevices-for-biosensing-and-bioimaging
#7
Qi Chen, Sharon Rozovsky, Wilfred Chen
Outer membrane vesicles (OMVs) are proteoliposomes derived from the outer membrane and periplasmic space of many Gram-negative bacteria including E. coli as part of their natural growth cycle. Inspired by the natural ability of E. coli to sort proteins to both the exterior and interior of OMVs, we reported here a one-pot synthesis approach to engineer multi-functionalized OMV-based sensors for both antigen binding and signal generation. SlyB, a native lipoprotein, was used a fusion partner to package nanoluciferase (Nluc) within OMVs, while a previously developed INP-Scaf3 surface scaffold was fused to the Z-domain for antibody recruiting...
June 21, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28632380/recent-progress-in-nanomaterial-based-optical-aptamer-assay-for-the-detection-of-food-chemical-contaminants
#8
Lingyi Lan, Yao Yao, Jianfeng Ping, Yibin Ying
Food chemical contaminant is a major factor to cause foodborne diseases and can do harm to human health. Hence, to develop robust, easy, and sensitive methods for rapid evaluation of food chemical contaminants is highly desirable. Nanomaterial-based optical aptasensors combined with the advantages of the high selectivity of optical detection techniques, excellent stability of aptamer, and the unique properties of nanomaterials have been recognized as useful tools for routine biosensing applications. The recent progress in nanomaterial-based optical aptamer assays to determine food chemical contaminants including heavy metals, toxins, pesticides, and antibiotics are presented in this paper...
June 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28627546/theoretical-study-of-gas-and-solvent-phase-stability-and-molecular-adsorption-of-noncanonical-guanine-bases-on-graphene
#9
Nabanita Saikia, Shashi P Karna, Ravindra Pandey
The gas and solvent phase stability of noncanonical (Gua)n nucleobases is investigated in the framework of dispersion-corrected density functional theory (DFT). The calculated results strongly support the high tendency for the dimerization of (Gua)n bases in both gas and solvent phases. An interplay between intermolecular and bifurcated H-bonds is suggested to govern the stability of (Gua)n bases which bears a correlation with the description of dispersion correction terms employed in the DFT calculations. For example, a higher polarity is predicted for (Gua)n bases by the dispersion-corrected DFT in contrast to the non-polar nature of (Gua)3 and (Gua)4 predicted by the hybrid meta-GGA calculations...
June 19, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28625736/use-of-bioconjugation-with-cytochrome-p450-enzymes
#10
REVIEW
Julie Ducharme, Karine Auclair
Bioconjugation, defined as chemical modification of biomolecules, is widely employed in biological and biophysical studies. It can expand functional diversity and enable applications ranging from biocatalysis, biosensing and even therapy. This review summarizes how chemical modifications of cytochrome P450 enzymes (P450s or CYPs) have contributed to improving our understanding of these enzymes. Genetic modifications of P450s have also proven very useful but are not covered in this review. Bioconjugation has served to gain structural information and investigate the mechanism of P450s via photoaffinity labeling, mechanism-based inhibition (MBI) and fluorescence studies...
June 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28624997/expression-and-purification-of-cytochrome-p450-55b1-from-chlamydomonas-reinhardtii-and-its-application-in-nitric-oxide-biosensing
#11
Bin Gong, Xiaosheng Liang, Yong Li, Qian Xiao, Panchun Yang, Yunhua Wu
Cytochrome P450 55B1 from Chlamydomonas reinhardtii is reported to function as a nitric oxide reductase (NOR). Here, we expressed the cytochrome P450 55B1 gene with an HIS-tag in E scherichia coli using a pET28a vector. The native protein was produced at a level of 1.59 μmol/g of total protein, with approximately 85% of the P450 being soluble. The CYP55B1 protein was characterized spectrally and purified by a HIS-trap column. This procedure allowed recovery of 45% of the expressed protein and CYP55B1 with a specific content of 0...
June 17, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28616986/on-a-chip-biosensing-based-on-all-dielectric-nanoresonators
#12
Ozlem Yavas, Mikael Svedendahl, Paulina Dobosz, Vanesa Sanz, Romain Quidant
Nanophotonics has become a key enabling technology in biomedicine with great promises in early diagnosis and less invasive therapies. In this context, the unique capability of plasmonic noble metal nanoparticles to concentrate light on the nanometer scale has widely contributed to biosensing and enhanced spectroscopy. Recently, high-refractive index dielectric nanostructures featuring low loss resonances have been proposed as a promising alternative to nanoplasmonics, potentially offering better sensing performances along with full compatibility with the microelectronics industry...
June 16, 2017: Nano Letters
https://www.readbyqxmd.com/read/28613808/sensitivity-enhanced-wearable-active-voiceprint-sensor-based-on-cellular-polypropylene-piezoelectret
#13
Wenbo Li, Sheng Zhao, Nan Wu, Junwen Zhong, Bo Wang, Shizhe Lin, Shuwen Chen, Fang Yuan, Hulin Jiang, Yongjun Xiao, Bin Hu, Jun Zhou
Wearable active sensors have extensive applications in mobile biosensing and human-machine interaction but require good flexibility, high sensitivity, excellent stability and self-powered feature. In this work, cellular polypropylene (PP) piezoelectret was chosen as the core material of a sensitivity-enhanced wearable active voiceprint sensor (SWAVS) to realize voiceprint recognition. By virtue of dipole orientation control method, the air layers in piezoelectret were efficiently utilized and the current sensitivity was enhanced (from 1...
June 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28612952/a-nanoparticle-decorated-biomolecule-responsive-polymer-enables-robust-signaling-cascade-for-biosensing
#14
Zuan-Tao Lin, Jianhua Gu, Chien-Hung Li, T Randall Lee, Lixin Xie, Shuo Chen, Piao-Yang Cao, Shan Jiang, Yulin Yuan, Xia Hong, Hongting Wang, Dezhi Wang, Xifan Wang, Gang-Biao Jiang, Mikala Heon, Tianfu Wu
To meet the increasing demands for ultrasensitivity in monitoring trace amounts of low-abundance early biomarkers or environmental toxins, the development of a robust sensing system is urgently needed. Here, a novel signal cascade strategy is reported via an ultrasensitive polymeric sensing system (UPSS) composed of gold nanoparticle (gNP)-decorated polymer, which enables gNP aggregation in polymeric network and electrical conductance change upon specific aptamer-based biomolecular recognition. Ultralow concentrations of thrombin (10(-18) m) as well as a low molecular weight anatoxin (165 Da, 10(-14) m) are detected selectively and reproducibly...
June 14, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28609719/near-infrared-photoluminescence-biosensing-platform-with-gold-nanorods-over-gallium-arsenide-nanohorn-array
#15
Yiming Zhang, Tao Jiang, Longhua Tang
The near-infrared (NIR) optical detection of biomolecules with high sensitivity and reliability have been expected, however, it is still a challenge. In this work, we present a gold nanorods (AuNRs)-over-gallium arsenide nanohorn-like array (GaAs NHA) system that can be used for the ultrasensitive and specific NIR photoluminescence (PL) detection of DNA and proteins. The fabrication of GaAs NHA involved the technique of colloidal lithography and inductively coupled plasma dry etching, yielding large-area and well-defined nanostructural array, and exhibiting an improved PL emission compared to the planar GaAs substrate...
June 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28609717/a-real-time-range-potentiostat-coupled-to-nano-au-modified-microband-electrode-array-for-high-speed-stripping-determination-of-human-blood-lead
#16
Wen Zhang, Yiwei Xu, Xiaobo Zou, Ping Wang
A novel method of real-time-range measurement, characterized by pre-sampling forecast and balanced-range switching, is introduced in this study. According to this method, raw current signals in biosensing procedures may be measured and recorded with real-time-optimized instrumental settings. A low-cost and high-performance potentiostat is developed to validate the proposed method. The transient process of real-time-range measurement is investigated to optimize sampling interval and circuit parameters. Typical time consumption of a sampling cycle is less than 100μs, which makes high-speed and real-time-range measurement possible...
June 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28609716/accelerating-bacterial-growth-detection-and-antimicrobial-susceptibility-assessment-in-integrated-picoliter-droplet-platform
#17
Aniruddha M Kaushik, Kuangwen Hsieh, Liben Chen, Dong Jin Shin, Joseph C Liao, Tza-Huei Wang
There remains an urgent need for rapid diagnostic methods that can evaluate antibiotic resistance for pathogenic bacteria in order to deliver targeted antibiotic treatments. Toward this end, we present a rapid and integrated single-cell biosensing platform, termed dropFAST, for bacterial growth detection and antimicrobial susceptibility assessment. DropFAST utilizes a rapid resazurin-based fluorescent growth assay coupled with stochastic confinement of bacteria in 20 pL droplets to detect signal from growing bacteria after 1h incubation, equivalent to 2-3 bacterial replications...
June 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28608953/simultaneous-visualization-and-quantitation-of-multiple-steroid-hormones-based-on-signal-amplified-biosensing-with-duplex-molecular-recognition
#18
Yaning Tan, Xiaoxia Hu, Meng Liu, Xinwen Liu, Xiaobo Lv, Zhihao Li, Jie Wang, Quan Yuan
The simultaneous quantitation of multiple steroid hormones in real-time is of great importance for medical diagnosis. In this study, a portable hormone biosensor based on duplex molecular recognition coupled with a signal-amplified substrate was successfully developed for the simultaneous visualization and quantitation of multiple steroid hormones. Aptamer-functionalized upconversion nanoparticles (UCNPs) with different emission peaks are immobilized on the photonic crystal (PC) substrate as the nanoprobes, leading to the specific and simultaneous assay of multiple steroid hormones...
June 13, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28608630/precisely-tailored-dna-nanostructures-and-their-theranostic-applications
#19
REVIEW
Bing Zhu, Lihua Wang, Jiang Li, Chunhai Fan
A critical challenge in nanotechnology is the limited precision and controllability of the structural parameters, which brings about concerns in uniformity, reproducibility and performance. Self-assembled DNA nanostructures, as a newly emerged type of nano-biomaterials, possess low-nanometer precision, excellent programmability and addressability. They can precisely arrange various molecules and materials to form spatially ordered complex, resulting in unambiguous physical or chemical properties. Because of these, DNA nanostructures have shown great promise in numerous biomedical theranostic applications...
June 13, 2017: Chemical Record: An Official Publication of the Chemical Society of Japan ... [et Al.]
https://www.readbyqxmd.com/read/28605185/soft-matter-printed-circuit-board-with-uv-laser-micropatterning
#20
Tong Lu, Eric Markvicka, Yichu Jin, Carmel Majidi
When encapsulated in elastomer, micropatterned traces of Ga-based liquid metal (LM) can function as elastically deformable circuit wiring that provides mechanically robust electrical connectivity between solid state elements (e.g. transistors, processors, sensor nodes). However, LM-microelectronics integration is currently limited by challenges in rapid fabrication of LM circuits and the creation of vias between circuit terminals and the I/O pins of packaged electronics. In this study we address both with a unique layup for soft-matter electronics in which traces of liquid-phase Ga-In eutectic (EGaIn) are patterned with UV laser micromachining (UVLM)...
June 12, 2017: ACS Applied Materials & Interfaces
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