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https://www.readbyqxmd.com/read/28432431/initial-cell-adhesion-of-three-cell-types-in-the-presence-and-absence-of-serum-proteins
#1
Martina Verdanova, Pavla Sauerova, Ute Hempel, Marie Hubalek Kalbacova
With the development of a wide range of new biomaterials for the sensing of different cell behaviour, it is important to consider whether the cells tested in vitro are in direct contact with the material or whether cell-biomaterial contact is mediated by an interfacial layer of proteins originating from the culture medium or from the cells themselves. Thus, this study describes the differences between the cell adhesion mediated by proteins originating from foetal bovine serum and without the presence of such proteins 2 h following cell seeding exemplarily with different cell types (an osteoblastic cell line, primary fibroblasts, and mesenchymal stem cells)...
April 21, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28432309/fano-resonance-with-high-local-field-enhancement-under-azimuthally-polarized-excitation
#2
Wuyun Shang, Fajun Xiao, Weiren Zhu, Hongsen He, Malin Premaratne, Ting Mei, Jianlin Zhao
Being an enabling technology for applications such as ultrasensitive biosensing and surface enhanced spectroscopy, enormous research interests have been focused on further boosting the local field enhancement at Fano resonance. Here, we demonstrate a plasmonic Fano resonance resulting from the interference between a narrow magnetic dipole mode and a broad electric dipole mode in a split-ring resonator (SRR) coupled to a nanoarc structure. Strikingly, when subjected to an azimuthally polarized beam (APB) excitation, the intensity enhancement becomes more than 60 times larger than that for a linearly polarized beam (LPB)...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28427012/label-free-electrochemical-biosensing-of-small-molecule-inhibition-on-o-glcnac-glycosylation
#3
Yu Yang, Yuxin Gu, Bin Wan, Xiaomin Ren, Liang-Hong Guo
O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) plays a critical role in modulating protein function in many cellular processes and human diseases such as Alzheimer's disease and type II diabetes, and has emerged as a promising new target. Specific inhibitors of OGT could be valuable tools to probe the biological functions of O-GlcNAcylation, but a lack of robust nonradiometric assay strategies to detect glycosylation, has impeded efforts to identify such compounds. Here we have developed a novel label-free electrochemical biosensor for the detection of peptide O-GlcNAcylation using protease-protection strategy and electrocatalytic oxidation of tyrosine mediated by osmium bipyridine as a signal reporter...
April 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28426196/chiral-inorganic-nanostructures
#4
Wei Ma, Liguang Xu, André F de Moura, Xiaoling Wu, Hua Kuang, Chuanlai Xu, Nicholas A Kotov
The field of chiral inorganic nanostructures is rapidly expanding. It started from the observation of strong circular dichroism during the synthesis of simple nanoparticles (NPs) and their assemblies mediated by biomolecules and expanded to sophisticated synthetic protocols involving nanostructures from metals, semiconductors, ceramics, and nanocarbons. Besides the well-established chirality transfer from bioorganic molecules, other methods to impart handedness to nanoscale matter specific to inorganic materials were discovered, including three-dimentional lithography, multiphoton chirality transfer, polarization effects in nanoscale assemblies, and others...
April 20, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28421739/an-ultra-efficient-cap-exchange-protocol-to-compact-biofunctional-quantum-dots-for-sensitive-ratiometric-biosensing-and-cell-imaging
#5
Weili Wang, Yuan Guo, Christian Tiede, Siyuan Chen, Michal Kopytynski, Yifei Kong, Alexander N Kulak, Darren Tomlinson, Rongjun Chen, Michael John McPherson, Dejian Zhou
An ultra-efficient cap-exchange protocol (UCEP) that can convert hydrophobic quantum dots (QDs) into stable, biocompatible and aggregation-free water-dispersed ones at a ligand:QD molar ratio (LQMR) as low as 500, some 20-200 fold less than most literature methods has been developed. The UCEP works conveniently with air-stable lipoic acid (LA)-based ligands by exploiting tris(2-carboxylethyl phosphine) based rapid in situ reduction. The resulting QDs are compact (hydrodynamic radius, Rh, <4.5 nm), bright (retaining >90% of original fluorescence), resist nonspecific adsorption of proteins and display good stability in biological buffers even with high salt content (e...
April 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28419916/a-sensitive-label-free-immunosensor-for-detection-%C3%AE-fetoprotein-in-whole-blood-based-on-anticoagulating-magnetic-nanoparticles
#6
Tingting Xu, Bo Chi, Fan Wu, Shangshang Ma, Shuyue Zhan, Meihui Yi, Hong Xu, Chun Mao
Accurate values of tumor markers in blood play an especially important role in the diagnosis of illness. Here, based on the combination of three techniques include anticoagulant technology, nanotechnology and biosensing technology, a sensitive label-free immunosensor with anti-biofouling electrode for detection α-Fetoprotein (AFP) in whole blood was developed by anticoagulating magnetic nanoparticles. The obtained products of Fe3O4-ɛ-PL-Hep nanoparticles were characterized by fourier transform infrared (FT-IR) spectra, transmission electron microscopy (TEM), ζ-potential and vibrating sample magnetometry (VSM)...
April 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28417176/functional-gold-nanoparticles-for-optical-affinity-biosensing
#7
Tomáš Špringer, Xue Chadtová Song, Maria Laura Ermini, Josefína Lamačová, Jiří Homola
Functional gold nanoparticles (AuNPs) are commonly used to enhance the response of optical affinity biosensors. In this work, we investigated the effect of preparation conditions on functional properties of AuNPs functionalized with antibody (Ab-AuNPs), specifically AuNPs with antibody against carcinoembryonic antigen (CEA) covalently attached via carboxy-terminated oligo-ethylene thiolate linker layer. The following parameters of preparation of Ab-AuNP have been found to have a significant effect on Ab-AuNP performance in affinity biosensors: the time of reaction of activated AuNPs with antibody, concentrations of antibody and amino-coupling reagents, and composition of immobilization buffer (molarity and salt content)...
April 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28415884/alcohol-quantification-recent-insights-into-amperometric-enzyme-biosensors
#8
Vinita Hooda, Vikas Kumar, Anjum Gahlaut, Vikas Hooda
Biosensors are the switching channels that make sense. The biosensors have found an empirical role in health applications (e.g. clinical diagnostics) as they represent the technological counterpart of living senses. On a global scale, alcohol analysis is indispensable for criminal justice systems, monitoring medical conditions of HIV patients & pregnant women as well as public safety issues regarding pilots, metro drivers, doctors etc. For addressing the clinical and toxicological problems, much advancement in the improvement of biosensors have been witnessed in recent decades...
April 17, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28413763/relationship-between-side-chain-polarity-and-the-self-assembly-characteristics-of-perylene-diimide-derivatives-in-aqueous-solution
#9
Jurgen Schill, Lech-Gustav Milroy, Jody A M Lugger, Albertus P H J Schenning, Luc Brunsveld
Perylene-3,4,9,10-tetracarboxylic acid diimides (PDIs) have recently gained considerable interest for water-based biosensing applications. PDIs have been studied intensively in the bulk state, but their physical properties in aqueous solution in interplay with side-chain polarity are, however, poorly understood. Therefore, three perylene diimide based derivatives were synthesized to study the relationship between side-chain polarity and their self-assembly characteristics in water. The polarity of the side chains was found to dictate the size and morphology of the formed aggregates...
April 2017: ChemistryOpen
https://www.readbyqxmd.com/read/28412660/gold-nanoparticle-based-low-limit-of-detection-love-wave-biosensor-for-carcinoembryonic-antigens
#10
Shuangming Li, Ying Wan, Yan Su, Chunhai Fan, Venkat R Bhethanabotla
In this work, a Love wave biosensing platform is described for detecting cancer-related biomarker carcinoembryonic antigen (CEA). An ST 90°-X quartz Love wave device with a layer of SiO2 waveguide was combined with gold nanoparticles (Au NPs) to amplify the mass loading effect of the acoustic wave sensor to achieve a limit of detection of 37pg/mL. The strategy involves modifying the Au NPs with anti-CEA antibody conjugates to form nanoprobes in a sandwich immunoassay. The unamplified detection limit of the Love wave biosensor is 9...
April 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28411650/armoring-enzymes-by-metal-organic-frameworks-by-the-coprecipitation-method
#11
Miao Hou, Jun Ge
Enzymatic processes have great potential for applications in industrial biocatalysis, biosensing, and biomedical engineering. However, poor stability and difficulty in the reuse of enzymes still create challenges for their use in catalysis and other applications. In recent years, enzyme-incorporated metal-organic frameworks (enzyme@MOF) have emerged as a new type of armored enzymes and are thought to be an appealing solution to the above challenges. To date, the preparation of enzyme-MOF composites can be divided into three categories, including physical adsorption, covalent conjugation, and coprecipitation...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28409624/rapid-surface-functionalization-of-hydrogen-terminated-silicon-by-alkyl-silanols
#12
Jorge Escorihuela, Han Zuilhof
Surface functionalization of inorganic semiconductor substrates, particularly silicon, has focused attention toward many technologically important applications, involving photovoltaic energy, biosensing and catalysis. For such modification processes, oxide-free (H-terminated) silicon surfaces are highly required, and different chemical approaches have been described in the past decades. However, their reactivity is often poor, requiring long reaction times (2-18 h) or the use of UV light (10-30 min). Here, we report a simple and rapid surface functionalization for H-terminated Si(111) surfaces using alkyl silanols...
April 14, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28406226/fast-optoelectric-printing-of-plasmonic-nanoparticles-into-tailored-circuits
#13
José A Rodrigo
Plasmonic nanoparticles are able to control light at nanometre-scale by coupling electromagnetic fields to the oscillations of free electrons in metals. Deposition of such nanoparticles onto substrates with tailored patterns is essential, for example, in fabricating plasmonic structures for enhanced sensing. This work presents an innovative micro-patterning technique, based on optoelectic printing, for fast and straightforward fabrication of curve-shaped circuits of plasmonic nanoparticles deposited onto a transparent electrode often used in optoelectronics, liquid crystal displays, touch screens, etc...
April 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28405933/detection-of-p53-gene-mutation-single-base-mismatch-using-a-fluorescent-silver-nanoclusters
#14
Morteza Hosseini, Shiva Mohammadi, Yasaman-Sadat Borghei, Mohammad Reza Ganjali
P53 mutation was detected through the application of a biosensing approach based on the decrease in the fluorescence of oligonucleotide-templated silver nanoclusters (DNA-AgNCs). To this end specific DNA scaffolds of two various nucleotide fragments were used. One of the scaffolds was enriched with two cytosine sequence fragment (C12). This led to DNA-AgNCs with a fluorescence intensity through chemical reduction, while the other scaffold acted as the probe fragment (5- GTAGATGGCCATGGCGCGGACGCGGGTG-3). This latter scaffold selectively bound to the specific p53 site...
April 13, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28405363/an-experimental-study-of-the-putative-mechanism-of-a-synthetic-autonomous-rotary-dna-nanomotor
#15
K E Dunn, M C Leake, A J M Wollman, M A Trefzer, S Johnson, A M Tyrrell
DNA has been used to construct a wide variety of nanoscale molecular devices. Inspiration for such synthetic molecular machines is frequently drawn from protein motors, which are naturally occurring and ubiquitous. However, despite the fact that rotary motors such as ATP synthase and the bacterial flagellar motor play extremely important roles in nature, very few rotary devices have been constructed using DNA. This paper describes an experimental study of the putative mechanism of a rotary DNA nanomotor, which is based on strand displacement, the phenomenon that powers many synthetic linear DNA motors...
March 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28398631/intraband-optical-activity-of-semiconductor-nanocrystals
#16
Anvar S Baimuratov, Nikita V Tepliakov, Yurii K Gun'Ko, Alexey G Shalkovskiy, Alexander V Baranov, Anatoly V Fedorov, Ivan D Rukhlenko
Here we review our three recently developed analytical models describing the intraband optical activity of semiconductor nanocrystals, which is induced by screw dislocations, ionic impurities, or irregularities of the nanocrystal surface. The models predict that semiconductor nanocrystals can exhibit strong optical activity upon intraband transitions and have large dissymmetry of magnetic-dipole absorption. The developed models can be used to interpret experimental circular dichroism spectra of nanocrystals and to advance the existing techniques of enantioseparation, biosensing, and chiral chemistry...
April 11, 2017: Chirality
https://www.readbyqxmd.com/read/28398221/amyloid-fibrils-from-hemoglobin
#17
Nadishka Jayawardena, Manmeet Kaur, Smitha Nair, Jenny Malmstrom, David Goldstone, Leonardo Negron, Juliet A Gerrard, Laura J Domigan
Amyloid fibrils are a class of insoluble protein nanofibers that are formed via the self-assembly of a wide range of peptides and proteins. They are increasingly exploited for a broad range of applications in bionanotechnology, such as biosensing and drug delivery, as nanowires, hydrogels, and thin films. Amyloid fibrils have been prepared from many proteins, but there has been no definitive characterization of amyloid fibrils from hemoglobin to date. Here, nanofiber formation was carried out under denaturing conditions using solutions of apo-hemoglobin extracted from bovine waste blood...
April 11, 2017: Biomolecules
https://www.readbyqxmd.com/read/28397497/semi-permeable-functional-dna-encapsulated-nanocapsules-as-protective-bioreactors-for-biosensing-in-living-cells
#18
Cheng-Yi Hong, Shu-Xian Wu, Shi-Hua Li, Hong Liang, Shan Chen, Juan Li, Huang-Hao Yang, Weihong Tan
The development of functional DNA-based nanosensors in living cells has experienced some design challenges, including, for example, poor cellular uptake, rapid nuclease degradation and high false positives. Herein, we designed selectively permeable poly (methacrylic acid) (PMA) nanocapsules to encapsulate functional DNAs for metal ions and small-molecules sensing in living cells. Since functional DNAs are concentrated in the nanocapsules, an increasing reaction rate is obtained in vitro. During endocytosis, polymeric capsules simultaneously improve cellular uptake of functional DNAs and preserve their structural integrity inside the confined capsule space...
April 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28395255/universal-ratiometric-electrochemical-biosensing-platform-based-on-mesoporous-platinum-nanocomposite-and-nicking-endonuclease-assisted-dna-walking-strategy
#19
Qinli Pu, Junlong Li, Juhui Qiu, Xuanhua Yang, Yi Li, Dan Yin, Xinyuan Zhang, Yiyi Tao, Shangchun Sheng, Guoming Xie
The occurrence and development of many complex diseases are associated with various molecules, whose contents are rarely in the early stage of the disease. Thus a universal platform for the ultrasensitive detection of multilevel biomarkers should be developed. In this study, we introduced an electrochemical biosensing system based on nicking endonuclease (Nt.BbvCI) assisted DNA walking strategy. We successfully constructed a universal signal-off-on ratiometric electrochemical biosensor for various biomolecules, including small molecules, nucleic acids, and proteins, by progressively optimizing the schematics (schemes 1, 2, and 3)...
April 1, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28392839/recent-advances-in-microscale-western-blotting
#20
Brittany J Sanders, Daniel C Kim, Robert C Dunn
Western blotting is a ubiquitous tool used extensively in the clinical and research settings to identify proteins and characterize their levels. It has rapidly become a mainstay in research laboratories due to its specificity, low cost, and ease of use. The specificity arises from the orthogonal processes used to identify proteins. Samples are first separated based on size and then probed with antibodies specific for the protein of interest. This confirmatory approach helps avoid pitfalls associated with antibody cross-reactivity and specificity issues...
October 21, 2016: Analytical Methods: Advancing Methods and Applications
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