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Gil Henkin, Daniel Berard, Francis Stabile, Marjan Shayegan, Jason S Leith, Sabrina R Leslie
We present a dynamically adjustable nanofluidic platform for formatting the conformations of and visualizing the interaction kinetics between biomolecules in solution, offering new time resolution and control of the reaction processes. This platform extends Convex Lens-induced Confinement (CLiC), a technique for imaging molecules under confinement, by introducing a system for in-situ modification of the chemical environment; this system uses a deep microchannel to diffusively exchange reagents within the nanoscale imaging region, whose height is fixed by a nanopost array...
October 21, 2016: Analytical Chemistry
D Kaba, D Berte, D Ta Bi Tra, J Tellería, P Solano, J-P Dujardin
This is the first study to explore the potential of various geometric morphometrics methods to help the morphological diagnostic of tsetse species, vectors of human and animal trypanosomiases in sub-Saharan Africa. We compared landmarks, semilandmarks and outlines techniques on male and female samples of species, and suggested adapted strategies according to the countries and their own Glossina fauna. We could compare up to 7 taxa belonging to the three main subgenera of the Glossina genus: Nemorhina (5 species), Glossina (1 species) and Austenina (1 species)...
October 17, 2016: Infection, Genetics and Evolution
Mahtab Tavasoli, Abass Al-Momany, Xin Wang, Laiji Li, John Clark Edwards, Barbara J Ballermann
The chloride intracellular channel (CLIC) 5A is expressed at very high levels in renal glomeruli, in both endothelial cells (EC) and podocytes. CLIC5A stimulates Rac1- and PI[4,5]P2-dependent ERM (ezrin, radixin, moesin) activation. ERM proteins, in turn, function in lumen formation and in the development of actin-based cellular projections. In mice lacking CLIC5A, ERM phosphorylation is profoundly reduced in podocytes, but preserved in glomerular EC. Since glomerular EC also express CLIC4, we reasoned if CLIC4 activates ERM proteins like CLIC5A, then CLIC4 could compensate for the CLIC5A loss in glomerular EC...
August 31, 2016: American Journal of Physiology. Renal Physiology
Paul Worley, Ian Couper, Roger Strasser, Lisa Graves, Beth-Ann Cummings, Richard Woodman, Pamela Stagg, David Hirsh
CONTEXT: Longitudinal integrated clerkships (LICs) represent a model of the structural redesign of clinical education that is growing in the USA, Canada, Australia and South Africa. By contrast with time-limited traditional block rotations, medical students in LICs provide comprehensive care of patients and populations in continuing learning relationships over time and across disciplines and venues. The evidence base for LICs reveals transformational professional and workforce outcomes derived from a number of small institution-specific studies...
September 2016: Medical Education
V Nikolaou, E Papadavid, A Patsatsi, M Siakantaris, A Economidi, L Marinos, T Koletsa, E Georgiou, V Pappa, A Stratigos, C Antoniou
BACKGROUND: Mycosis fungoides (MF) is an indolent cutaneous lymphoma with excellent prognosis at early stages and much poorer outcome during disease progression. Old age, male sex and folliculotropism have been proposed as relevant prognostic factors, however, their exact effect remains debatable. OBJECTIVE: To evaluate MF prognostic indicators and survival rates in a Greek population. METHODS: Prognostic variables effecting survival rates were studied in 473 MF patients diagnosed and treated by two academic referral centers in Greece...
August 24, 2016: British Journal of Dermatology
Bojing Jia, Tse-Luen Wee, Colton G Boudreau, Daniel J Berard, Adiel Mallik, David Juncker, Claire M Brown, Sabrina R Leslie
Here we present a high-throughput, parallelized cytoindentor for local compression of live cells. The cytoindentor uses convex lens-induced confinement (CLiC) to indent micrometer-sized areas in single cells and/or populations of cells with submicron precision. This is accomplished using micropatterned poly(dimethylsiloxane) (PDMS) films that are adhered to a convex lens to create arrays of extrusions referred to here as "posts." These posts caused local deformation of subcellular regions without any evidence of cell lysis upon CLiC indentation...
2016: BioTechniques
Catherine Metayer, Eleni Petridou, Juan Manuel Mejía Aranguré, Eve Roman, Joachim Schüz, Corrado Magnani, Ana Maria Mora, Beth A Mueller, Maria S Pombo de Oliveira, John D Dockerty, Kathryn McCauley, Tracy Lightfoot, Emmanouel Hatzipantelis, Jérémie Rudant, Janet Flores-Lujano, Peter Kaatsch, Lucia Miligi, Catharina Wesseling, David R Doody, Maria Moschovi, Laurent Orsi, Stefano Mattioli, Steve Selvin, Alice Y Kang, Jacqueline Clavel
The association between tobacco smoke and acute myeloid leukemia (AML) is well established in adults but not in children. Individual-level data on parental cigarette smoking were obtained from 12 case-control studies from the Childhood Leukemia International Consortium (CLIC, 1974-2012), including 1,330 AML cases diagnosed at age <15 years and 13,169 controls. We conducted pooled analyses of CLIC studies, as well as meta-analyses of CLIC and non-CLIC studies. Overall, maternal smoking before, during, or after pregnancy was not associated with childhood AML; there was a suggestion, however, that smoking during pregnancy was associated with an increased risk in Hispanics (odds ratio = 2...
August 15, 2016: American Journal of Epidemiology
E Viedma-Guiard, P Agüero, L Crespo-Araico, C Estévez-Fraga, G Sánchez-Díez, J L López-Sendón, I Aviles-Olmos, G García-Ribas, M L Palacios Romero, J Masjuan Vallejo, J C Martínez-Castrillo, A Alonso-Cánovas
INTRODUCTION: The clinical problems of patients with movement disorders (MD) are complex, and the duration and frequency of face-to-face consultations may be insufficient to meet their needs. We analysed the implementation of an e-mail-based query service for our MD unit's patients and their primary care physicians (PCPs). METHODS: We retrospectively reviewed all consecutive emails sent and received over a period of 4 months, one year after implementation of the e-mail inquiry system...
July 26, 2016: Neurología: Publicación Oficial de la Sociedad Española de Neurología
Justin L Sewell, Christy K Boscardin, John Q Young, Olle Ten Cate, Patricia S O'Sullivan
OBJECTIVES: Few studies have investigated cognitive factors affecting learning of procedural skills in medical education. Cognitive load theory, which focuses on working memory, is highly relevant, but methods for measuring cognitive load during procedural training are not well understood. Using colonoscopy as an exemplar, we used cognitive load theory to develop a self-report instrument to measure three types of cognitive load (intrinsic, extraneous and germane load) and to provide evidence for instrument validity...
June 2016: Medical Education
Kanin Salao, Lele Jiang, Hui Li, Vicky W-W Tsai, Yasmin Husaini, Paul M G Curmi, Louise J Brown, David A Brown, Samuel N Breit
Intracellular chloride channel protein 1 (CLIC1) participates in inflammatory processes by regulating macrophage phagosomal functions such as pH and proteolysis. Here, we sought to determine if CLIC1 can regulate adaptive immunity by actions on dendritic cells (DCs), the key professional antigen presenting cells. To do this, we first generated bone marrow-derived DCs (BMDCs) from germline CLIC1 gene-deleted (CLIC1(-/-)) and wild-type (CLIC1(+/+)) mice, then studied them in vitro and in vivo We found phagocytosis triggered cytoplasmic CLIC1 translocation to the phagosomal membrane where it regulated phagosomal pH and proteolysis...
2016: Biology Open
Devasena Ponnalagu, Shubha Gururaja Rao, Jason Farber, Wenyu Xin, Ahmed Tafsirul Hussain, Kajol Shah, Soichi Tanda, Mark A Berryman, John C Edwards, Harpreet Singh
Chloride intracellular channel (CLICs) proteins show 60-70% sequence identity to each other, and exclusively localize to the intracellular organelle membranes and cytosol. In support of our recent publication, "Molecular identity of cardiac mitochondrial chloride intracellular channel proteins" (Ponnalagu et al., 2016) [1], it was important to characterize the specificity of different CLIC paralogs/ortholog (CLIC1, CLIC4, CLIC5 and DmCLIC) antibodies used to decipher their localization in cardiac cells. In addition, localization of CLICs in the other organelles such as endoplasmic reticulum (ER) of cardiomyocytes was established...
June 2016: Data in Brief
Erin L Marcotte, Thomas P Thomopoulos, Claire Infante-Rivard, Jacqueline Clavel, Eleni Th Petridou, Joachim Schüz, Sameera Ezzat, John D Dockerty, Catherine Metayer, Corrado Magnani, Michael E Scheurer, Beth A Mueller, Ana M Mora, Catharina Wesseling, Alkistis Skalkidou, Wafaa M Rashed, Stephen S Francis, Roula Ajrouche, Friederike Erdmann, Laurent Orsi, Logan G Spector
BACKGROUND: Results from case-control studies have shown an increased risk of acute lymphoblastic leukaemia (ALL) in young children born by caesarean delivery, and prelabour caesarean delivery in particular; however, an association of method of delivery with childhood leukaemia subtypes has yet to be established. We therefore did a pooled analysis of data to investigate the association between childhood leukaemia and caesarean delivery. METHODS: We pooled data from 13 case-control studies from the Childhood Leukemia International Consortium done in nine countries (Canada, Costa Rica, Egypt, France, Germany, Greece, Italy, New Zealand, and the USA) for births from 1970-2013...
April 2016: Lancet Haematology
Xinwei Zhao, Lina Xu, Lingli Zheng, Lianhong Yin, Yan Qi, Xu Han, Youwei Xu, Jinyong Peng
BACKGROUND: We previously reported the effect of dioscin on human gastric carcinoma SGC-7901 cells, but its effects on other gastric cancers are still unknown. PURPOSE: The present paper aimed to demonstrate the activity of dioscin against human gastric carcinoma MGC-803 and MKN-45. STUDY DESIGN: In our study, MGC-803 and MKN-45 cells were used to examine the effects of dioscin on human gastric carcinoma in vitro. The effects of dioscin against human gastric carcinoma in vivo were accomplished by the xenografts of MGC-803 cells in BALB/c nude mice...
March 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
S Nishad, Anu Ghosh
Humans are continually exposed to ionizing radiation from natural as well as anthropogenic sources. Though biological effects of high dose radiation exposures have been well accepted, studies on low-to-moderate dose exposures (in the range of 50-500 mGy) have been strongly debated even as researchers continue to search for elusive 'radiation signatures' in humans. Proteins are considered as dynamic functional players that drive cellular responses. However, there is little proteomic information available in context of human exposure to ionizing radiation...
February 2016: Mutation Research. Genetic Toxicology and Environmental Mutagenesis
Khondker R Hossain, Heba Al Khamici, Stephen A Holt, Stella M Valenzuela
CLIC1 is a Chloride Intracellular Ion Channel protein that exists either in a soluble state in the cytoplasm or as a membrane bound protein. Members of the CLIC family are largely soluble proteins that possess the intriguing property of spontaneous insertion into phospholipid bilayers to form integral membrane ion channels. The regulatory role of cholesterol in the ion-channel activity of CLIC1 in tethered lipid bilayers was previously assessed using impedance spectroscopy. Here we extend this investigation by evaluating the influence of cholesterol on the spontaneous membrane insertion of CLIC1 into Langmuir film monolayers prepared using 1-palmitoyl-2-oleoylphosphatidylcholine, 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-ethanolamine and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine alone or in combination with cholesterol...
2016: Membranes
Haowei Xue, Jinsen Lu, Renxiang Yuan, Jinli Liu, Yehai Liu, Kaile Wu, Jing Wu, Juan Du, Bing Shen
BACKGROUND: Human head and neck squamous carcinoma is the 6th most prevalent carcinoma worldwide. Although many novel therapies have been developed, the clinical treatment for patients remains non-ideal. Chloride intracellular channel 4 (CLIC4), one of the seven members of the CLIC family, is a newly found Cl(-) channel that participates in various biological processes, including cellular apoptosis and differentiation. Accumulating evidence has revealed the significant role of CLIC4 in regulating the apoptosis of different cancer cells...
2016: Cell & Bioscience
Devasena Ponnalagu, Shubha Gururaja Rao, Jason Farber, Wenyu Xin, Ahmed Tafsirul Hussain, Kajol Shah, Soichi Tanda, Mark Berryman, John C Edwards, Harpreet Singh
Emerging evidences demonstrate significance of chloride channels in cardiac function and cardioprotection from ischemia-reperfusion (IR) injury. Unlike mitochondrial potassium channels sensitive to calcium (BKCa) and ATP (KATP), molecular identity of majority of cardiac mitochondrial chloride channels located at the inner membrane is not known. In this study, we report the presence of unique dimorphic chloride intracellular channel (CLIC) proteins namely CLIC1, CLIC4 and CLIC5 as abundant CLICs in the rodent heart...
March 2016: Mitochondrion
Alice Truong, Cassandre Yip, Alexandra Paye, Silvia Blacher, Carine Munaut, Christophe Deroanne, Agnès Noel, Nor Eddine Sounni
Membrane type 4 matrix metalloproteinase (MT4-MMP) [matrix metalloproteinase (MMP) 17] is a GPI-anchored membrane-type MMP expressed on the cell surface of human breast cancer cells. In triple-negative breast cancer cells, MT4-MMP promotes primary tumour growth and lung metastases. Although the trafficking and internalization of the transmembrane membrane type 1 MMP have been extensively investigated, little is known about the regulatory mechanisms of the GPI-anchored MT4-MMP. Here, we investigated the fate and cellular trafficking of MT4-MMP by analysing its homophilic complex interactions, internalization and recycling dynamics as compared with an inert form, MT4-MMP-E249A...
February 2016: FEBS Journal
O Buchmueller, M Citron, J Ellis, S Guha, J Marrouche, K A Olive, K de Vries, Jiaming Zheng
We discuss the potential impacts on the CMSSM of future LHC runs and possible [Formula: see text] and higher-energy proton-proton colliders, considering searches for supersymmetry via  [Formula: see text] events, precision electroweak physics, Higgs measurements and dark matter searches. We validate and present estimates of the physics reach for exclusion or discovery of supersymmetry via [Formula: see text] searches at the LHC, which should cover the low-mass regions of the CMSSM parameter space favoured in a recent global analysis...
2015: European Physical Journal. C, Particles and Fields
Vasiliki Tasiopoulou, Dimitrios Magouliotis, Evgeniy I Solenov, Georgios Vavougios, Paschalis-Adam Molyvdas, Konstantinos I Gourgoulianis, Chrissi Hatzoglou, Sotirios G Zarogiannis
BACKGROUND: Chloride Intracellular Channels (CLICs) are contributing to the regulation of multiple cellular functions. CLICs have been found over-expressed in several malignancies, and therefore they are currently considered as potential drug targets. The goal of our study was to assess the gene expression levels of the CLIC's 1-6 in malignant pleural mesothelioma (MPM) as compared to controls. METHODS: We used gene expression data from a publicly available microarray dataset comparing MPM versus healthy tissue in order to investigate the differential expression profile of CLIC 1-6...
December 2015: Computational Biology and Chemistry
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