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https://www.readbyqxmd.com/read/29679865/generation-and-characterization-of-hagfish-variable-lymphocyte-receptor-b-against-glycoprotein-of-viral-hemorrhagic-septicemia-virus-vhsv
#1
Jung Seok Lee, Jaesung Kim, Se Pyeong Im, Si Won Kim, Jassy Mary S Lazarte, Jae Wook Jung, Tae Won Gong, Young Rim Kim, Jeong Ho Lee, Hyoung Jun Kim, Tae Sung Jung
Variable lymphocyte receptors B (VLRBs) are non-immunoglobulin components of the humoral immune system in jawless vertebrates including hagfish (Eptatretus burgeri) and lamprey (Petromyzon marinus). Hagfish VLRBs consist of leucine rich repeat (LRR) modules with a superhydrophobic C-terminal tail, the latter of which leads to extremely low expression levels in recombinant protein technology. Here, we present an artificially oligomerized VLRB (arVLRB) that conjugates via the C4bp oligomerization domain derived from human C4b-binding protein (hC4bp) rather than the superhydrophobic tail...
April 18, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29679725/achyranthis-bidentatae-radix-enhanced-articular-distribution-and-anti-inflammatory-effect-of-berberine-in-sanmiao-wan-using-an-acute-gouty-arthritis-rat-model
#2
Juan Wu, Jingya Li, Wei Li, Bei Sun, Jin Xie, Wenming Cheng, Qunlin Zhang
ETHNOPHARMACOLOGICAL RELEVANCE: Sanmiao Wan (SMW) has been a basic prescription employed for the treatment for gout in the clinic since Yuan dynasty. Achyranthis bidentatae radix (ABR) is designed as a lower-guiding drug in SMW to augment the articular accumulation of active ingredients and improve the anti-inflammatory effect. AIM OF THE STUDY: Present study was undertaken to investigate the dose-response relationship of berberine in SMW between the articular concentration and anti-inflammatory effect in the knee joint under the lower-guiding of ABR...
April 18, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29679509/pharmacokinetic-properties-of-wogonin-and-its-herb-drug-interactions-with-docetaxel-in-rats-with-mammary-tumors
#3
Ting Wang, Fangyi Long, Gang Jiang, Hong Cai, Qian Jiang, Kai Cheng, Zhiqiang Hu, Yuxi Wang
Docetaxel, frequently used for the treatment of breast cancer, is mainly metabolized via hepatic cytochrome P450 (CYP) 3A in humans and is also a substrate of P-glycoprotein (P-gp). Wogonin has been shown to be able to modulate the activities of CYPs and P-gp, and it could be served as an adjuvant chemotherapeutic agent. However, the impacts of co-administration of wogonin and docetaxel on their pharmacokinetics have not been studied for lacking analytical method of their simultaneous measurement. In the present study, we established an HPLC-MS/MS method for simultaneous measurement of wogonin and docetaxel in rat plasma, and it was then utilized to explore the pharmacokinetics of wogonin and the herb-drug interactions between wogonin and docetaxel after the combined administration in rats with mammary tumors...
April 21, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29678950/mapping-interaction-sites-on-human-chemokine-receptors-by-deep-mutational-scanning
#4
Jeremiah D Heredia, Jihye Park, Riley J Brubaker, Steven K Szymanski, Kevin S Gill, Erik Procko
Chemokine receptors CXCR4 and CCR5 regulate WBC trafficking and are engaged by the HIV-1 envelope glycoprotein gp120 during infection. We combine a selection of human CXCR4 and CCR5 libraries comprising nearly all of ∼7000 single amino acid substitutions with deep sequencing to define sequence-activity landscapes for surface expression and ligand interactions. After consideration of sequence constraints for surface expression, known interaction sites with HIV-1-blocking Abs were appropriately identified as conserved residues following library sorting for Ab binding, validating the use of deep mutational scanning to map functional interaction sites in G protein-coupled receptors...
April 20, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29678884/physiological-temperatures-reduce-dimerization-of-dengue-and-zika-virus-recombinant-envelope-proteins
#5
Stephan T Kudlacek, Lakshmanane Premkumar, Stefan W Metz, Ashutosh Tripathy, Andrey A Bobkov, Alexander Matthew Payne, Stephen Graham, James A Brackbill, Michael J Miley, Aravinda M de Silva, Brian Kuhlman
The spread of dengue (DENV) and Zika virus (ZIKV) is a major public health concern. The primary target of antibodies (Abs) that neutralize DENV and ZIKV is the envelope (E) glycoprotein, and there is interest in using soluble recombinant E (sRecE) proteins as subunit vaccines. However, the most potent neutralizing antibodies against DENV and ZIKV recognize epitopes on the virion surface that span two or more E proteins. Therefore, to create effective DENV and ZIKV vaccines, presentation of these quaternary epitopes may be necessary...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678879/potassium-is-a-trigger-for-conformational-change-in-the-fusion-spike-of-an-enveloped-rna-virus
#6
Emma K Punch, Samantha Hover, Henry Tw Blest, Jack Fuller, Roger Hewson, Juan Fontana, Jamel Mankouri, John N Barr
Many enveloped viruses enter cells through the endocytic network, from which they must subsequently escape through fusion of viral and endosomal membranes. This membrane fusion is mediated by virus-encoded spikes that respond to the changing endosomal environment, which triggers conformational change in the spike that initiate the fusion process. Several fusion triggers have been identified and include pH, membrane composition and endosome-resident proteins and these cues dictate when and where viral fusion occurs...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678725/cloning-and-characterization-of-a-second-lamprey-pituitary-glycoprotein-hormone-thyrostimulin-gpa2-gpb5
#7
Krist N Hausken, Belen Tizo, Michal Shpilman, Shannon Barton, Wayne Decatur, David Plachetzki, Scott Kavanaugh, Sabah Ul-Hasan, Berta Levavi-Sivan, Stacia A Sower
A novel heterodimeric glycoprotein hormone (GpH) comprised of alpha (GpA2) and beta (GpB5) subunits was discovered in 2002 and called thyrostimulin for its ability to activate the TSH receptor in mammals, but its central function in vertebrates has not been firmly established. We report here the cloning and expression of lamprey (l)GpB5, and its ability to heterodimerize with lGpA2 to form a functional l-thyrostimulin. The full-length cDNA of lGpB5 encodes 174 amino acids with ten conserved cysteine residues and one glycosylation site that is conserved with other vertebrate GpB5 sequences...
April 17, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29678614/an-animal-s-sex-influences-the-effects-of-the-excipient-peg-400-on-the-intestinal-p-gp-protein-and-mrna-levels-which-has-implications-for-oral-drug-absorption
#8
Yang Mai, Liu Dou, Sudaxshina Murdan, Abdul Basit
There is a growing body of evidence which suggests that formerly regarded "inert" pharmaceutical excipients have the potential to influence oral drug bioavailability. The solubilizing agent polyethylene glycol 400 (PEG 400), for instance, has a sex-specific effect on P-glycoprotein (P-gp)-mediated drug bioavailability. We hypothesized that such an effect could be via PEG-induced alteration of P-gp activity and/or expression to different extents in males and females. To test this hypothesis in vivo, we investigated the influence of orally administered PEG 400 on the protein content and mRNA expression of P-gp in different regions of the gastrointestinal tract in male and female rats...
April 17, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29678592/retina-compatible-interactions-and-effective-modulation-of-blood-ocular-barrier-p-gp-activity-by-third-generation-inhibitors-improves-ocular-penetration-of-loperamide
#9
Karthik Yadav Janga, Akshaya Tatke, Surabhi Shukla, Surya P Lamichhane, Bharathi Avula, XiangDi Wang, Monica M Jablonski, Ikhlas A Khan, Soumyajit Majumdar
Effective drug delivery to the deeper ocular tissues remains an unresolved conundrum mainly due to the expression of multidrug resistance (MDR) efflux proteins, beside tight junction proteins, in the blood ocular barriers (BOB). Hence, the purpose of the current research was to investigate the ability of the third-generation efflux protein inhibitors, elacridar (EQ) and tariquidar (TQ), to diminish P-glycoprotein (P-gp) mediated efflux transport of loperamide (LOP), a P-gp substrate, across the BOB in SD rats...
April 17, 2018: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29678555/monomeric-intermediates-formed-by-vesiculovirus-glycoprotein-during-its-low-ph-induced-structural-transition
#10
REVIEW
Abbas Abou-Hamdan, Laura Belot, Aurélie Albertini, Yves Gaudin
No abstract text is available yet for this article.
April 17, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29677181/exploiting-glycan-topography-for-computational-design-of-env-glycoprotein-antigenicity
#11
Wen-Han Yu, Peng Zhao, Monia Draghi, Claudia Arevalo, Christina B Karsten, Todd J Suscovich, Bronwyn Gunn, Hendrik Streeck, Abraham L Brass, Michael Tiemeyer, Michael Seaman, John R Mascola, Lance Wells, Douglas A Lauffenburger, Galit Alter
Mounting evidence suggests that glycans, rather than merely serving as a "shield", contribute critically to antigenicity of the HIV envelope (Env) glycoprotein, representing critical antigenic determinants for many broadly neutralizing antibodies (bNAbs). While many studies have focused on defining the role of individual glycans or groups of proximal glycans in bNAb binding, little is known about the effects of changes in the overall glycan landscape in modulating antibody access and Env antigenicity...
April 20, 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29677132/cd81-receptor-regions-outside-the-large-extracellular-loop-determine-hepatitis-c-virus-entry-into-hepatoma-cells
#12
Pia Banse, Rebecca Moeller, Janina Bruening, Lisa Lasswitz, Sina Kahl, Abdul G Khan, Joseph Marcotrigiano, Thomas Pietschmann, Gisa Gerold
Hepatitis C virus (HCV) enters human hepatocytes using four essential entry factors, one of which is human CD81 (hCD81). The tetraspanin hCD81 contains a large extracellular loop (LEL), which interacts with the E2 glycoprotein of HCV. The role of the non-LEL regions of hCD81 (intracellular tails, four transmembrane domains, small extracellular loop and intracellular loop) is poorly understood. Here, we studied the contribution of these domains to HCV susceptibility of hepatoma cells by generating chimeras of related tetraspanins with the hCD81 LEL...
April 20, 2018: Viruses
https://www.readbyqxmd.com/read/29676461/antiphospholipid-syndrome-characteristics-and-adverse-pregnancy-outcomes-after-20-weeks-of-pregnancy
#13
Rinat Gabbay-Benziv, Hadas Zafrir-Danieli, Dorit Blickstein, Anat Shmueli, Lina Salman, Eran Hadar
OBJECTIVE: To assess outcomes after 20 weeks of pregnancy according to autoantibody profile and clinical presentation of maternal antiphospholipid syndrome (APS). METHODS: The present retrospective cohort analysis included women diagnosed with APS at a tertiary medical center in Israel between January 1, 2012, and December 31, 2016. Anticardiolipin antibodies, anti-β2-glycoprotein antibodies, and lupus anticoagulant were assessed. Participants were stratified by type of APS (obstetric vs thrombotic), antibody profile, and antibody titer (low vs high)...
April 20, 2018: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/29675916/serum-apoa4-levels-predicted-the-progression-of-renal-impairment-in-t2dm
#14
Chao-Wen Cheng, Che-Chang Chang, Hsiu-Wen Chen, Ching-Yu Lin, Jin-Shuen Chen
BACKGROUND: Among multiple causes, diabetic nephropathy (DN) is the major underlying renal disease that leads to end-stage renal disease (ESRD), and early diagnosis can effectively prevent or delay the progression to ESRD. Therefore, the current study aimed to develop non-invasive, accurate detection markers. MATERIALS & METHODS: For this study, 62 diabetes mellitus (DM) patients, 59 DN patients, and 21 healthy controls (HCs) were recruited. All participants' serum samples were subjected to concavanalin (Con) A affinity chromatography, which utilizes glycoproteins to discover potential markers...
April 19, 2018: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29675803/drosophila-s2-cell-culture-in-a-wave-bioreactor-potential-for-scaling-up-the-production-of-the-recombinant-rabies-virus-glycoprotein
#15
Monize Caiado Decarli, Diogo Peres Dos Santos, Renato Mancini Astray, Daniella Cristina Ventini-Monteiro, Soraia Attie Calil Jorge, Daniela Matilde Correia, Juliana de Sá da Silva, Mayra Pereira Rocca, Hélio Langoni, Benedito Donizete Menozzi, Carlos Augusto Pereira, Claudio Alberto Torres Suazo
The transmembrane rabies virus glycoprotein (RVGP) is the main antigen of vaccine formulations used around the world to prevent rabies, the most lethal preventable infectious disease known. The objective of this work was to evaluate the potential of a bioreactor using wave-induced agitation in the initial steps of scaling up the rRVGP production process by a Drosophila melanogaster S2 cell line to produce rRVGP in sufficient quantities for immunization and characterization studies. Taking advantage of some remarkable features recognized in Drosophila S2 cells for scaling the culture process, a robust recombinant lineage (S2MtRVGPH-His) engineered by our group for the expression of rRVGP using a copper-inducible promoter was used in the bioreactor cultures...
April 19, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29675746/cabazitaxel-is-more-active-than-first-generation-taxanes-in-abcb1-cell-lines-due-to-its-reduced-affinity-for-p-glycoprotein
#16
George E Duran, Volker Derdau, Dietmar Weitz, Nicolas Philippe, Jörg Blankenstein, Jens Atzrodt, Dorothée Sémiond, Diego A Gianolio, Sandrine Macé, Branimir I Sikic
PURPOSE: The primary aim of this study was to determine cabazitaxel's affinity for the ABCB1/P-glycoprotein (P-gp) transporter compared to first-generation taxanes. METHODS: We determined the kinetics of drug accumulation and retention using [14 C]-labeled taxanes in multidrug-resistant (MDR) cells. In addition, membrane-enriched fractions isolated from doxorubicin-selected MES-SA/Dx5 cells were used to determine sodium orthovanadate-sensitive ATPase stimulation after exposure to taxanes...
April 19, 2018: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/29675740/identification-of-poly-n-acetyllactosamine-carrying-glycoproteins-from-hl-60-human-promyelocytic-leukemia-cells-using-a-site-specific-glycome-analysis-method-glyco-ridge
#17
Akira Togayachi, Azusa Tomioka, Mika Fujita, Masako Sukegawa, Erika Noro, Daisuke Takakura, Michiyo Miyazaki, Toshihide Shikanai, Hisashi Narimatsu, Hiroyuki Kaji
To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed. Glycopeptide-based structural analysis technology using mass spectrometry has been developed; however, complicated analyses of complex spectra obtained by multistage fragmentation are necessary, and sensitivity and throughput of the analyses are low. Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE)...
April 19, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29675623/influence-of-autoimmunity-and-inflammation-on-endothelial-function-and-thrombosis-in-systemic-lupus-erythematosus-patients
#18
Kamil Bugała, Adam Mazurek, Krzysztof Gryga, Monika Komar, Grzegorz Kopeć, Jacek Musiał, Piotr Podolec, Carlo Perricone, Wojciech Płazak
The aim of this study is to assess the relationship between autoimmunity and endothelial activation/damage (ICAM-1 and vWF serum levels) and the degree of prothrombotic activity (thrombin-antithrombin complexes-TAT serum levels) in SLE. In 60 clinically stable SLE patients, levels of the following parameters were estimated in their serum: lupus anticoagulant (LA), anticardiolipin antibodies in both IgG and IgM classes (aCL-IgG and aCL-IgM, respectively), antiβ2GPI antibodies in both IgG and IgM classes (antiβ2GPI-IgG and antiβ2GPI-IgM, respectively), ICAM, von Willebrand factor (vWF), TAT, CRP, C3c, C4, and IL-6...
April 20, 2018: Clinical Rheumatology
https://www.readbyqxmd.com/read/29675569/effect-of-zinc-sulfate-and-zinc-glycine-chelate-on-concentrations-of-acute-phase-proteins-in-chicken-serum-and-liver-tissue
#19
Łukasz Jarosz, Agnieszka Marek, Zbigniew Grądzki, Ewa Laskowska, Małgorzata Kwiecień
The aim of the study was to determine how inorganic and organic forms of zinc affect the concentrations of C-reactive protein (CRP), serum amyloid A (SAA), alpha-1-acid glycoprotein (α-1-AGP), haptoglobin (Hp), and transferrin (TRF) in the blood and liver tissue of 450 1-day-old Ross 308 chicken. Four experimental groups received one the following: inorganic zinc (ZnSO4 ), a zinc phytase enzyme supplement (ZnSO4 -F), organic zinc in combination with glycine (Zn-Gly), or organic zinc supplemented with phytase (Zn-Gly-F)...
April 19, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29674747/glycointeractions-in-bacterial-pathogenesis
#20
REVIEW
Jessica Poole, Christopher J Day, Mark von Itzstein, James C Paton, Michael P Jennings
Many important interactions between bacterial pathogens and their hosts are highly specific binding events that involve host or pathogen carbohydrate structures (glycans). Glycan interactions can mediate adhesion, invasion and immune evasion and can act as receptors for toxins. Several bacterial pathogens can also enzymatically alter host glycans to reveal binding targets, degrade the host cell glycans or alter the function of host glycoproteins. In recent years, high-throughput screening technologies, such as lectin, glycan and mucin microarrays, have transformed the field by identifying new bacterial-host glycointeractions, which are crucial for colonization, persistence and disease...
April 19, 2018: Nature Reviews. Microbiology
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