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https://www.readbyqxmd.com/read/28540817/effect-of-applied-voltage-duration-and-repetition-frequency-of-rf-pulses-for-pain-relief-on-temperature-spikes-and-electrical-field-a-computer-modelling-study
#1
Elżbieta Ewertowska, Borja Mercadal, Víctor Muñoz, Antoni Ivorra, Macarena Trujillo, Enrique Berjano
BACKGROUND: The thermal and electrical effects of pulsed radiofrequency (PRF) for pain relief can be controlled by modifying the characteristics of the RF pulses applied. Our goal was to evaluate the influence of such modifications on the thermal and electric performance in tissue. METHODS: A computational model was developed to compare the temperature and electric field time courses in tissue between a standard clinical protocol (45 V pulses, 20 ms duration, 2 Hz repetition frequency) and a new protocol (55 V pulses, 5 ms duration, 5 Hz repetition frequency) with a higher applied electric field but a smaller impact on temperature alterations in tissue...
May 16, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/28535550/high-voltage-pulsed-radiofrequency-for-the-treatment-of-refractory-neuralgia-of-the-infraorbital-nerve-a-prospective-double-blinded-randomized-controlled-study
#2
Fang Luo, Tao Wang, Ying Shen, Lan Meng, Jingjing Lu, Nan Ji
BACKGROUND: A recent study showed that 50% of patients who suffered from refractory neuralgia of the infraorbital nerve obtained satisfactory efficacy after pulsed radiofrequency (PRF) treatment. A pilot study showed that increasing the output voltage of PRF significantly improved the efficacy for trigeminal neuralgia; however, whether increasing the output voltage of PRF can improve the treatment outcomes for neuralgia of the infraorbital nerve is unknown. OBJECTIVE: To evaluate the efficacy and safety of high voltage PRF treatment in comparison with standard voltage PRF for neuralgia of the infraorbital nerve...
May 2017: Pain Physician
https://www.readbyqxmd.com/read/28529414/comparison-of-efficacy-of-autologous-platelet-rich-fibrin-versus-saline-dressing-in-chronic-venous-leg-ulcers-a-randomised-controlled-trial
#3
Anirudh Somani, Reena Rai
BACKGROUND: Venous leg ulcer is a chronic condition, and various treatment modalities are available. Platelet-rich fibrin (PRF) is one of the newer modalities and it contains fibroblast growth factor (GF), vascular endothelial GF, angiopoitin and platelet-derived GF which enhances the wound healing. Hence, we conducted a randomised controlled trial to compare the efficacy of PRF versus saline dressing in chronic venous leg ulcers. AIM: This study aims to compare the efficacy of autologous PRF with saline dressing in patients with chronic venous leg ulcer and to compare the mean reduction in ulcer area at the end of 4 weeks...
January 2017: Journal of Cutaneous and Aesthetic Surgery
https://www.readbyqxmd.com/read/28522581/conformational-dynamics-of-the-frameshift-stimulatory-structure-in-hiv-1
#4
Dustin B Ritchie, Tonia R Cappellano, Collin Tittle, Negar Rezajooei, Logan Rouleau, William Ka Sikkema, Michael T Woodside
Programmed ribosomal frameshifting (PRF) in HIV-1 is thought to be stimulated by a hairpin in the mRNA, although a pseudoknot-like triplex has also been proposed. Because the conformational dynamics of the stimulatory structure under tension applied by the ribosomal helicase during translation may play an important role in PRF, we used optical tweezers to apply tension to the HIV stimulatory structure and monitor its unfolding and refolding dynamics. The folding and unfolding kinetics and energy landscape of the hairpin were measured by ramping the force on the hairpin up and down, providing a detailed biophysical characterisation...
May 18, 2017: RNA
https://www.readbyqxmd.com/read/28517960/a-rapid-seedling-resistance-assay-identifies-wild-tomato-lines-that-are-resistant-to-pseudomonas-syringae-pv-tomato-race-1
#5
Jana A Hassan, Yan-Ming Jane Zhou, Jennifer D Lewis
Bacterial speck caused by Pseudomonas syringae has historically been controlled by the Pto/Prf gene cluster. Emerging strains like P. syringae pv. tomato (Pst) race 1 overcome resistance conferred by Pto/Prf, and can cause serious crop loss under appropriate environmental conditions. We developed a rapid assay to screen wild tomato seedlings for resistance to Pst race 1. We established the seedling resistance assay using the well-characterized Pst race 0 strain, PstDC3000, that is recognized in tomato cultivars carrying Pto/Prf (PtoR) and causes disease in isogenic lines lacking this cluster (PtoS)...
May 18, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28516051/evaluation-of-mr-thermometry-with-proton-resonance-frequency-method-at-7t
#6
Ping Wang
Quantitative and non-invasive temperature mapping using magnetic resonance imaging (MRI) provides a unique way to measure temperature evolution inside biological tissues. The method is widely used in thermal ablation procedures with magnetic fields at or below 3T. In this paper, the sensitivity of the MRI thermometry at 7T was studied using a proton resonance frequency (PRF)-based technique. We first used an agarose gel phantom with MR-compatible thermometry to calibrate the temperature coefficient, and then this temperature coefficient was employed to measure the internal temperature in both ex vivo (beef muscle) and in vivo (rat) experiments using focused ultrasound heating...
April 2017: Quantitative Imaging in Medicine and Surgery
https://www.readbyqxmd.com/read/28516050/towards-mri-temperature-mapping-in-real-time-the-proton-resonance-frequency-method-with-undersampled-radial-mri-and-nonlinear-inverse-reconstruction
#7
Zhongshuai Zhang, Thomas Michaelis, Jens Frahm
BACKGROUND: Optimal control of minimally invasive interventions by hyperthermia requires dynamic temperature mapping at high temporal resolution. METHODS: Based on the temperature-dependent shift of the proton resonance frequency (PRF), this work developed a method for real-time MRI thermometry which relies on highly undersampled radial FLASH MRI sequences with iterative image reconstruction by regularized nonlinear inversion (NLINV). As a first step, the method was validated with use of a temperature phantom and ex vivo organs (swine kidney) subjected to heating by warm water or a pulsed laser source...
April 2017: Quantitative Imaging in Medicine and Surgery
https://www.readbyqxmd.com/read/28509340/prf-based-matrices-to-improve-angiogenesis-in-an-in-vitro-co-culture-model-for-bone-tissue-engineering
#8
Eva Dohle, Karima El Bagdadi, Robert Sader, Joseph Choukroun, C James Kirkpatrick, Shahram Ghanaati
In the context of prevascularization strategies for tissue engineering purposes, co-culture systems consisting of outgrowth endothelial cells (OECs) and primary osteoblasts (pOBs) have been established as a promising in vitro tool to study regeneration mechanisms and to identify factors that might positively influence repair processes like wound healing or angiogenesis. The development of autologous injectable platelet rich fibrin (PRF), which can be generated from peripheral blood in a minimal invasive procedure, fulfils several requirements for clinically applicable cell-based tissue engineering strategies...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28498470/novel-role-of-semaphorin%C3%A2-3a-in-the-growth-and-progression-of-hepatocellular-carcinoma
#9
Xuedong Li, Qing Chen, Dan Yin, Shiming Shi, Lei Yu, Shaolai Zhou, Erbao Chen, Zhengjun Zhou, Yinghong Shi, Jia Fan, Jian Zhou, Zhi Dai
Semaphorin 3A (SEMA3A), a secretory protein, is a founding member of the semaphorin family and functions in both the biological behavior of tumor cells and the modulation of tumor-associated macrophages. However, the role of SEMA3A in hepatocellular carcinoma (HCC) is still not well established. In the present study, we investigated the expression levels of SEMA3A in 80 HCC tissues and cell lines, using RT-qPCR, western blotting and immunohistochemistry. Expression profile analysis revealed that SEMA3A was significantly overexpressed in human HCC patients and positively correlated with the metastatic potential of HCC cells...
May 2, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28498077/the-effect-of-platelet-rich-fibrin-membrane-on-the-repair-of-perforated-tympanic-membrane-an-experimental-study
#10
Nuray Ensari, Özer Erdem Gür, Mehmet Türker Öztürk, Dinç Süren, Ömer Tarık Selçuk, Üstün Osma
CONCLUSION: Platelet-rich fibrin (PRF) membrane could be used successfully in the repair of tympanic membrane perforation and wound healing. OBJECTIVES: To evaluate the effect of platelet-rich fibrin membrane in the repair of perforated tympanic membrane. METHODS: After otoscopic examination, a 3-mm perforation was made in the posterior quadrant of both tympanic membranes of 50 adult male Sprague-Dawley rats. Venous blood was withdrawn from the rats, then centrifuged...
February 22, 2017: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/28489660/effects-of-growth-factors-from-platelet-rich-fibrin-on-the-bone-regeneration
#11
Jin Kim, Yooseok Ha, Nak Heon Kang
Platelet-rich fibrin (PRF) has simple manufacturing process without risk of immunologic rejection or infection by additional materials. This study was designed to investigate the effects of platelet-derived growth factors and transforming growth factors-β in PRF on the proliferation and differentiation of osteoblasts. The authors analyzed platelet-derived growth factors and transforming growth factors-β in normal human serum (NHS) and PRF by enzyme-linked immunosorbent assay quantitatively and those in PRF group were significantly increased when compared with NHS...
May 9, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28489280/synthesis-and-evaluation-of-cytotoxic-activities-of-artemisinin-derivatives
#12
Qian Sun, Jin Wang, Yao Li, Jingjing Zhuang, Qian Zhang, Xiao Sun, Dequn Sun
Artemisinin is naturally occurring antimalarials which has shown potent anticancer activity. In the present work, a new kind of artemisinin derivatives with piperazino group were synthesized. The cytotoxic activities of derivatives 5a-5d were evaluated by MTT assay against ten cell lines. The result showed that 5a-5d were more effective in inhibiting cancer cells growth than artemisinin. 5d was the most active against HepG2 and PLC-PRF-5 cells, and presented no cytotoxicity on L-02 cells. Hoechst33342 staining and flow cytometry experiment revealed that 5d could induce HepG2 and PLC-PRF-5 cells apoptosis...
May 10, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28476390/achilles-tendon-open-surgical-treatment-with-platelet-rich-fibrin-matrix-augmentation-biomechanical-evaluation
#13
Federica Alviti, Michele Gurzì, Valter Santilli, Marco Paoloni, Roberto Padua, Andrea Bernetti, Marco Bernardi, Massimiliano Mangone
The relationship between surgical technique and ankle biomechanical properties after surgery for acute rupture of the Achilles tendon (ATR) has not yet been fully investigated. Platelet-rich fibrin (PRF) matrices seem to play a central role in the complex processes of tendon healing. Our aim was to analyze the biomechanical characteristics, stiffness, and mechanical work of the ankle during walking in patients who had undergone surgery after ATR with and without PRF augmentation. We performed a retrospective review of all consecutive patients who had been treated with surgical repair after ATR...
May 2017: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/28473242/evaluation-of-postoperative-complications-after-mandibular-third-molar-surgery-with-the-use-of-platelet-rich-fibrin-a-systematic-review-and-meta-analysis
#14
REVIEW
J V Dos S Canellas, F G Ritto, P J D Medeiros
The current literature was reviewed to analyze the effects of platelet-rich fibrin (PRF) on postoperative complications after mandibular third molar surgery (pain, alveolar osteitis, swelling, and bone healing). A comprehensive literature search was performed up to 2016 in the PubMed/MEDLINE, Cochrane Library, LILACS, and ScienceDirect databases and the grey literature. Additional records were identified through manual and reference searches. The full-text articles of potentially relevant studies were reviewed; only randomized clinical trials were included...
May 1, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28466249/mechanical-and-degradation-properties-of-advanced-platelet-rich-fibrin-a-prf-concentrated-growth-factors-cgf-and-platelet-poor-plasma-derived-fibrin-pptf
#15
Kazushige Isobe, Taisuke Watanebe, Hideo Kawabata, Yutaka Kitamura, Toshimitsu Okudera, Hajime Okudera, Kohya Uematsu, Kazuhiro Okuda, Koh Nakata, Takaaki Tanaka, Tomoyuki Kawase
BACKGROUND: Fibrin clot membranes prepared from advanced platelet-rich fibrin (A-PRF) or concentrated growth factors (CGF), despite their relatively rapid biodegradability, have been used as bioactive barrier membranes for alveolar bone tissue regeneration. As the membranes degrade, it is thought that the growth factors are gradually released. However, the mechanical and degradable properties of these membranes have not well been characterized. The purpose of this study was to mechanically and chemically characterize these membranes...
December 2017: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/28465712/a-novel-hairpin-library-based-approach-to-identify-nbs-lrr-genes-required-for-effector-triggered-hypersensitive-response-in-nicotiana-benthamiana
#16
Cyril Brendolise, Mirco Montefiori, Romain Dinis, Nemo Peeters, Roy D Storey, Erik H Rikkerink
BACKGROUND: PTI and ETI are the two major defence mechanisms in plants. ETI is triggered by the detection of pathogen effectors, or their activity, in the plant cell and most of the time involves internal receptors known as resistance (R) genes. An increasing number of R genes responsible for recognition of specific effectors have been characterised over the years; however, methods to identify R genes are often challenging and cannot always be translated to crop plants. RESULTS: We present a novel method to identify R genes responsible for the recognition of specific effectors that trigger a hypersensitive response (HR) in Nicotiana benthamiana...
2017: Plant Methods
https://www.readbyqxmd.com/read/28458932/uncommon-odontogenic-orocutaneous-fistula-of-the-jaw-treated-with-platelet-rich-fibrin
#17
Kani Bilginaylar
Orocutaneous fistula (OCF) of dental origin is a relatively rare condition and continues to be a challenging diagnosis. Misdiagnosis of OCF usually leads to unnecessary and noneffective treatment. A 21-year-old male referred with a complaint of a lesion on the chin which was misdiagnosed as a carbuncle (lesion of nonodontogenic origin) by a physician. After radiological examination, there was a lesion around the apical region of right central incisor. These findings indicated a sinus tract associated with dental origin...
2017: Case Reports in Dentistry
https://www.readbyqxmd.com/read/28458929/effect-of-bone-regeneration-with-mineralized-plasmatic-matrix-for-implant-placement-in-aesthetic-zone
#18
Khadija Amine, Amina Gharibi, Azeddine Hsaine, Jamila Kissa
Bone volume is one of the key factors to be considered when evaluating implant placement. When the bone volume is insufficient, implant placement could be conditioned by the necessity of preforming bone grafting procedures to compensate bone loss. Various grafting procedures can be used with different bone substitute. Mineralized Plasmatic Matrix (MPM) is one of these grafting materials, used to maintain or regenerate the socket's volume. In MPM, the autologous blood products highly concentrated in platelets and fibrin in a liquid state are combined with a bone substitute...
2017: Case Reports in Dentistry
https://www.readbyqxmd.com/read/28454244/proliferation-of-sphere-forming-hepatocellular-carcinoma-cells-is-suppressed-in-a-medium-without-glucose-and-arginine-but-with-galactose-and-ornithine
#19
Minoru Tomizawa, Fuminobu Shinozaki, Yasufumi Motoyoshi, Takao Sugiyama, Shigenori Yamamoto, Naoki Ishige
Resistance to sorafenib in hepatocellular carcinoma (HCC) cells exhibiting stemness was evaluated using a sphere formation assay. A hepatocyte selection medium (HSM) deficient in glucose and arginine was used to suppress the proliferation of cell spheres composed of HLF and PLC/PRF/5 HCC cells, which were subjected to a sphere formation assay. Cell spheres were cultured with sorafenib and subjected to a cell proliferation assay and the expression levels of cytochrome P450 (CYP3A4) were analyzed in RNA extracted from sphere-forming cells using reverse transcription-quantitative polymerase chain reaction...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28452623/advantages-of-autologous-platelet-rich-fibrin-membrane-on-gingival-crevicular-fluid-growth-factor-levels-and-periodontal-healing-a-randomized-split-mouth-clinical-study
#20
Taner Arabaci, Oguz Kose, Mevlut Albayrak, Yasin Cicek, Alper Kizildag
AIM: To evaluate the contributions of platelet rich fibrin (PRF) combined with conventional flap surgery on the growth factor levels in gingival crevicular fluid (GCF) and periodontal healing. MATERIALS AND METHOD: 26 patients (52 sites) with chronic periodontitis were treated either with autologous PRF with open flap debridement (OFD+PRF) or open flap debridement (OFD) alone. Growth factor levels in GCF at baseline and 2, 4 and 6 weeks postoperatively were analyzed, and clinical parameters such as probing depth (PD), relative attachment level (RAL) and gingival margin level (GML) at baseline and 9 months after surgery were compromised...
April 28, 2017: Journal of Periodontology
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