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https://www.readbyqxmd.com/read/29345045/the-in-vitro-release-of-cytokines-and-growth-factors-from-fibrin-membranes-produced-through-horizontal-centrifugation
#1
E S Lourenço, C F A B Mourão, P E C Leite, J M Granjeiro, M D Calasans-Maia, G G Alves
Platelet-rich fibrin (PRF) membranes are biomaterials widely used for therapeutic purposes, and canonically produced through the processing of peripheral blood with fixed-angle rotor centrifuges. In this work, we evaluate the in vitro stability and release of cytokines and growth factors when these biomaterials are produced with a horizontal swing-out clinical centrifuge. Membranes produced from the blood of 14 donors were morphologically evaluated by scanning electron microscopy and fluorescence microscopy, and their stability was assessed by photographic recording after incubation in culture medium for up to 28 days...
January 18, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29336623/the-effect-of-phytosterol-rich-fraction-from-palm-fatty-acid-distillate-on-blood-serum-lipid-profile-of-dyslipidemia-rats
#2
Kgs Ahmadi, Huda Oktafa, Teti Estiasih
Phytosterol-rich fraction (PRF) obtained from palm fatty acid distillate (PFAD) was investigated for its effect on blood serum lipid profile of dyslipidemia rats. Dyslipidemia was induced by force feeding cholesterol to five groups of rats; one group was a control or normal group. Cholesterol force-fed groups were treated with 0, 10, 20, 30, and 40 mg PRF/kg/day for 4 weeks. All groups of rats were fed standard diet. A normal group was fed standard diet without PRF treatment and cholesterol force feeding...
January 16, 2018: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/29334078/high-prf-ultrafast-sliding-compound-doppler-imaging-fully-qualitative-and-quantitative-analysis-of-blood-flow
#3
Jinbum Kang, Won Seuk Jang, Yangmo Yoo
Ultrafast compound Doppler imaging based on plane-wave excitation (UCDI) can be used to evaluate cardiovascular diseases using high frame rates. In particular, it provides a fully quantifiable flow analysis over a large region of interest with high spatio-temporal resolution. However, the pulse-repetition frequency (PRF) in the UCDI method is limited for high-velocity flow imaging since it leverages between the number of plane-wave angles (<i>N</i>) and acquisition time. In this paper, we present high PRF ultrafast sliding compound Doppler imaging method (HUSDI) to improve quantitative flow analysis...
January 15, 2018: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29333099/pulsed-radiofrequency-applied-to-the-sciatic-nerve-improves-neuropathic-pain-by-down-regulating-the-expression-of-calcitonin-gene-related-peptide-in-the-dorsal-root-ganglion
#4
Hao Ren, Hailong Jin, Zipu Jia, Nan Ji, Fang Luo
Background: Clinical studies have shown that applying pulsed radiofrequency (PRF) to the neural stem could relieve neuropathic pain (NP), albeit through an unclear analgesic mechanism. And animal experiments have indicated that calcitonin gene-related peptide (CGRP) expressed in the dorsal root ganglion (DRG) is involved in generating and maintaining NP. In this case, it is uncertain whether PRF plays an analgesic role by affecting CGRP expression in DRG. Methods: Rats were randomly divided into four groups: Groups A, B, C, and D...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29330457/spatially-selective-responses-to-kanizsa-and-occlusion-stimuli-in-human-visual-cortex
#5
Benjamin de Haas, Dietrich Samuel Schwarzkopf
Early visual cortex responds to illusory contours in which abutting lines or collinear edges imply the presence of an occluding surface, as well as to occluded parts of an object. Here we used functional magnetic resonance imaging (fMRI) and population receptive field (pRF) analysis to map retinotopic responses in early visual cortex using bar stimuli defined by illusory contours, occluded parts of a bar, or subtle luminance contrast. All conditions produced retinotopic responses in early visual field maps even though signal-to-noise ratios were very low...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29329442/conventional-radiofrequency-thermocoagulation-vs-pulsed-radiofrequency-neuromodulation-of-ganglion-impar-in-chronic-perineal-pain-of-nononcological-origin
#6
Hammad Usmani, G P Dureja, Roshan Andleeb, Nazia Tauheed, Naiyer Asif
Background: Chronic nononcological perineal pain has been effectively managed by ganglion Impar block. Chemical neurolysis, cryoablation, and radiofrequency ablation have been the accepted methods of blockade. Recently, pulsed radiofrequency, a novel variant of conventional radiofrequency, has been used for this purpose. Study Design: This was a prospective, randomized, double-blind study. Setting: Two different interventional pain management centers in India...
January 10, 2018: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/29328169/inverse-synthetic-aperture-ladar-demonstration-system-structure-imaging-processing-and-experiment-result
#7
Ning Wang, Ran Wang, Di Mo, Guangzuo Li, Keshu Zhang, Yirong Wu
A long-distance inverse synthetic aperture LADAR (ISAL) imaging experiment outdoors over 1 km for cooperative targets is demonstrated, which gets a two-dimensional high-resolution image with resolution exceeding 2.5 cm. The system utilizes an electro-optic in-phase and quadrature modulator to output a linear frequency-modulated continuous waveform (LFMCW) with a bandwidth of 6 GHz and pulse repetition frequency (PRF) of 16.7 KHz. For the problem of the coherence of the laser, the effects of the coherent processing interval (CPI) and time delay of the local oscillator (LO) on the coherence are discussed...
January 10, 2018: Applied Optics
https://www.readbyqxmd.com/read/29326521/surgical-management-of-large-radicular-cyst-associated-with-mandibular-deciduous-molar-using-platelet-rich-fibrin-augmentation-a-rare-case-report
#8
Vijaya S Dhote, Nilima R Thosar, Sudhindra M Baliga, Priyanti Dharnadhikari, Poonam Bhatiya, Punit Fulzele
Radicular cysts arising from deciduous teeth are rare and usually cause a large bony defect. Autologous platelet-rich fibrin (PRF) is an easily available healing biomaterial in oral surgical defect with the new perspective of accelerated healing of a large bony defect. The present case is of unusually large radicular associated with neglected carious mandibular deciduous second molar in 10-year-old girl and its surgical management with PRF augmentation as a healing biomaterial in the bony defect. One-year follow-up showed uneventful healing and eruption of succedaneous tooth...
October 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/29326502/comparative-evaluation-of-platelet-rich-fibrin-with-connective-tissue-grafts-in-the-treatment-of-miller-s-class-i-gingival-recessions
#9
Sonam Mufti, Sarvagna Mayank Dadawala, Phoram Patel, Monali Shah, Deepak Harish Dave
Background: One of the most common aesthetic problem encountered in the field of periodontology is gingival recession, which is, perceived by the patients as increase in length of teeth. The treatment of buccal gingival recession is a common requirement due to aesthetic concern or root sensitivity. This study was planned to evaluate the efficacy of PRF membrane compared to that of CTG in Miller's class I gingival recessions. Materials and Methods: 32 sites with Miller's Class I gingival recessions, out of which 16 sites received PRF (test) and 16 sites received CTG (control)...
October 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/29317362/lumbar-dorsal-root-ganglion-block-as-a-prognostic-tool-prior-to-pulsed-radiofrequency-a-randomized-prospective-and-comparative-study-on-cost-effectiveness
#10
Cheng-Chia Lee, Ching-Jen Chen, Chien-Chen Chou, Hsin-Yi Wang, Wen-Yuh Chung, Giia-Sheun Peng, Ching-Po Lin
OBJECTIVES: In the study, we discuss the predictive value and cost-effectiveness of dorsal root ganglion (DRG) blocks prior to using dorsal root ganglion pulsed radiofrequency (DRG-PRF) in the treatment of low back pain. METHODS: The study comprised 60 patients with low back pain who were randomly assigned into two groups. Patients in Group 1 were screened using DRG block prior to DRG-PRF treatment for responders. Patients in Group 2 underwent DRG-PRF treatment without DRG block...
January 6, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29316445/does-intra-alveolar-application-of-chlorhexidine-gel-in-combination-with-platelet-rich-fibrin-have-an-advantage-over-application-of-platelet-rich-fibrin-in-decreasing-alveolar-osteitis-after-mandibular-third-molar-surgery-a-double-blinded-randomized-clinical
#11
Majid Eshghpour, Nasrin Danaeifar, Hamed Kermani, Amir Hossein Nejat
PURPOSE: To evaluate the effectiveness of chlorhexidine (CHX) gel and platelet-rich fibrin (PRF) compared with PRF alone in preventing the development of alveolar osteitis (AO). MATERIALS AND METHODS: In a double-blinded trial, patients undergoing surgical management of bilateral impacted mandibular third molars were randomly divided into 2 groups; 1 group received PRF in 1 extraction socket with the other socket as its control and the other group received 0.2% CHX gel plus PRF in 1 socket with the other socket serving as its control...
December 16, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29314065/platelet-rich-fibrin-combined-with-a-porous-hydroxyapatite-graft-for-the-treatment-of-3-wall-intrabony-defects-in-chronic-periodontitis-a-randomized-controlled-clinical-trial
#12
A R Pradeep, Pavan Bajaj, Nishanth S Rao, Esha Agarwal, Savitha B Naik
BACKGROUND: Porous hydroxyapatite (HA) bone grafting material has been used to fill periodontal intrabony defects (IBDs), resulting in clinically acceptable responses. Platelet-rich fibrin (PRF) is a leukocyte and platelet preparation that concentrates various polypeptide growth factors and, therefore, has the potential for use as regenerative treatment for periodontal defects. The present study aims to explore the clinical and radiographic effectiveness of autologous PRF versus PRF + HA in treatment of IBDs in patients with chronic periodontitis...
December 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/29304325/effects-of-leukocyte-and-platelet-rich-fibrin-alone-and-combined-with-hyaluronic-acid-on-pain-edema-and-trismus-after-surgical-extraction-of-impacted-mandibular-third-molars
#13
İbrahim Murat Afat, Emine Tuna Akdoğan, Onur Gönül
PURPOSE: In this prospective, randomized, double-blind controlled study, we evaluated the effects of leukocyte- and platelet-rich fibrin (L-PRF) alone and combined with a hyaluronic acid (HA) sponge on pain, edema, and trismus after mandibular third molar surgery. PATIENTS AND METHODS: In total, 60 patients were included in this study. The patients were randomly divided into 3 groups: L-PRF group (L-PRF was applied to the socket), L-PRF-plus-HA group (L-PRF combined with HA was applied to the socket), and control group (nothing was applied)...
December 13, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29288515/a-third-generation-oncolytic-herpes-simplex-virus-inhibits-the-growth-of-liver-tumors-in-mice
#14
Richi Nakatake, Masaki Kaibori, Yusuke Nakamura, Yoshito Tanaka, Hideyuki Matushima, Tadayoshi Okumura, Takashi Murakami, Yasushi Ino, Tomoki Todo, Masanori Kon
Multimodality therapies are used to manage patients with hepatocellular carcinoma (HCC), although advanced HCC is incurable. Oncolytic virus therapy is probably the next major breakthrough in cancer treatment. The third-generation oncolytic herpes simplex virus type 1 (HSV-1) T-01 kills tumor cells without damaging the surrounding normal tissues. Here we investigated the antitumor effects of T-01 on HCC and the host's immune response to HCC cells. The cytopathic activities of T-01 were tested in 14 human and one murine hepatoma cell lines in vitro...
December 30, 2017: Cancer Science
https://www.readbyqxmd.com/read/29285901/characterization-of-porcine-sapelovirus-isolated-from-japanese-swine-with-plc-prf-5-cells
#15
H Bai, J Liu, L Fang, M Kataoka, N Takeda, T Wakita, T-C Li
Porcine sapelovirus (PSV) is a causative agent of neurological disorders, fertility disorders and dermal lesions of swine. In this study, we isolated two PSV strains, Jpsv477 and Jpsv1315, from swine faecal specimens using a PLC/PRF/5 cell culture system. The PSV infection of PLC/PRF/5 cells induced a cytopathic effect (CPE). Two types of virus particles with identical diameter (~35 nm) but different densities (1.300 and 1.285 g/cm3 ) were observed in the cell culture supernatants. Analysis of the entire genome sequence of Jpsv477 and Jpsv1315 revealed that both strains possess 7,558 nucleotides and the poly (A) tail and have a typical PSV genome organization consisting of a 5' terminal untranslated region (5'UTR), a large open reading frame (ORF), and a 3' terminal untranslated region (3'UTR)...
December 28, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29284978/successful-surgical-management-of-palatogingival-groove-using-platelet-rich-fibrin-and-guided-tissue-regeneration-a-novel-approach
#16
J V Karunakaran, Susan Mathew Fenn, N Jayaprakash, N Ragavendran
Palatogingival groove also known as radicularlingual groove is a developmental anomaly involving the lingual surface of the maxillary incisors. They are inconspicuous, funnel-shaped, extend for varying distances on root surface and occur due to infolding of the hertwigs epithelial root sheath. This encourages adherence of microorganisms and plaque to levels significant for pathological changes resulting in endodontic and periodontal lesions. The variations in anatomy of the tooth as a cause of pulp necrosis in teeth of anterior maxillary segment should be considered by the clinician when other etiological factors are ruled out...
November 2017: Journal of Pharmacy & Bioallied Sciences
https://www.readbyqxmd.com/read/29284945/comparison-of-marginal-periosteal-pedicle-graft-and-bioactive-glass-with-platelet-rich-fibrin-and-bioactive-glass-in-the-treatment-of-intrabony-defects-a-clinicoradiographic-study
#17
M S Yasaswini, K V Prabhakara Rao, P Tanuja, Narendra Reddy Motakatla
Introduction and Objectives: The objective of the study was to compare clinically and radiographically the regenerative potential of marginal periosteal pedicle graft (MPPG) or platelet-rich fibrin (PRF) with a bioactive glass in the treatment of two- and three-wall intrabony defects. Methods: A total of 28 sites (bilateral or contralateral infrabony defects) from 14 patient were treated with MPPG (Experimental site A) and the other site was treated with PRF (Experimental site B)...
November 2017: Journal of Pharmacy & Bioallied Sciences
https://www.readbyqxmd.com/read/29279679/effectiveness-of-platelet-rich-fibrin-in-the-management-of-pain-and-delayed-wound-healing-associated-with-established-alveolar-osteitis-dry-socket
#18
Ashish Sharma, Nimish Aggarwal, Sanjay Rastogi, Rupshikha Choudhury, Siddhi Tripathi
Objective: To assess the efficacy of platelet-rich fibrin (PRF) on the pain and healing of the extraction socket related with established alveolar osteitis (dry socket, AO) after the removal of maxillary and mandibular molars. Materials and Methods: One hundred consecutive adult patients with age group ranging from 18 to 40 years along with established dry socket after maxillary and mandibular molar extractions who have not received any treatment for the same were included in this single-arm clinical trial...
October 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/29251327/survival-pathway-of-cholangiocarcinoma-via-akt-mtor-signaling-to-escape-raf-mek-erk-pathway-inhibition-by-sorafenib
#19
Kenta Yokoi, Akira Kobayashi, Hiroaki Motoyama, Masato Kitazawa, Akira Shimizu, Tsuyoshi Notake, Takahide Yokoyama, Tomio Matsumura, Michiko Takeoka, Shin-Ichi Miyagawa
Cholangiocarcinoma (CCC) is a strongly aggressive malignancy for which surgical resection is the only potential curative therapy. Sorafenib, a multikinase inhibitor of the RAF/MEK/ERK pathway, is a molecular-targeted drug that is approved for hepatocellular carcinoma (HCC) but not for CCC. The differences in signaling pathway characteristics under sorafenib treatment between HCC (HLF, Huh7, PLC/PRF/5) and CCC (RBE, YSCCC, Huh28) cell lines were therefore investigated using cell proliferation, western blotting, and apoptosis analyses...
February 2018: Oncology Reports
https://www.readbyqxmd.com/read/29249010/evaluating-the-main-sources-of-groundwater-pollution-in-the-southern-tehran-aquifer-using-principal-component-factor-analysis
#20
Hooman Ghahremanzadeh, Roohollah Noori, Akbar Baghvand, Touraj Nasrabadi
Determining the main sources of pollution (MSP) in groundwater is crucial to improve water quality (WQ) status. Field studies were conducted in this research, where five sampling campaigns were carried out from 36 wells in the southern Tehran aquifer. In all samples, WQ parameters were measured and evaluated regarding the Iranian drinking water standard (IDWS). Finally, by using the principal component factor analysis (PCFA), the probable MSP in the aquifer were determined. The results showed that all ions, total hardness, and total dissolved solids were above the IDWS...
December 16, 2017: Environmental Geochemistry and Health
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