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https://www.readbyqxmd.com/read/27001405/research-on-root-responses-to-pb-and-zn-combined-stress-of-carex-putuoshan
#1
Yong-Lin Hu, Jia-Lang Tan, Cheng-Long Wang, Zhan-Biao Yang, Yuan-Xiang Yang, Zhang Chen, Li-Jin Lin, Ying-Jun Wang, Gang Sun, Xue-Mei Zhu, Ji-Rong Shao, Mei-Liang Zhou
Pb hyper-accumulated Carex putuoshan was taken as experimental material and subjected to combined stress of Pb and Zn. The differential expression of proteins in their roots were analyzed by Proteomic Approach. The protein that was directly involved in the cellular defense under the Pb and Zn combined stress was separated, and expression of those genes was analyzed with Carex Evergold as control. The results were obtained by MALDI-TOF/MS analysis. After applying Pb and Zn combined stress, the expression of 9 protein spots (including 7 different proteins, 2 identical proteins, 1 unknown protein) in Carex putuoshan root was found to be significantly up-regulated...
2016: Protein and Peptide Letters
https://www.readbyqxmd.com/read/23473367/serum-biomarkers-reveal-long-term-cardiac-injury-in-isoproterenol-treated-african-green-monkeys
#2
Yashu Liu, Toufan Parman, Bridget Schneider, Benben Song, Amit K Galande, Dave Anderson, Jon Mirsalis
The assessment of cardiac toxicity is a major challenge in both drug development and clinical trials, and numerous marketed pharmaceuticals have been removed from the market due to unpredicted cardiac effects. Serum troponins are widely used indicators of cardiac injury; however, they are short-lived and have not been validated in preclinical animal models. In this study, we have used filter-aided sample preparation (FASP) and tandem mass tag (TMT) labeling to investigate serum protein alterations in isoproterenol-treated African green monkeys...
April 5, 2013: Journal of Proteome Research
https://www.readbyqxmd.com/read/16767285/-experimental-models-of-insulin-resistance-and-obesity-lessons-learned
#3
REVIEW
Mario Luís Ribeiro Cesaretti, Osvaldo Kohlmann Junior
For better understanding the role of each element involved in the physiopathology of obesity and insulin resistance, researchers can use experimental models, which may in controlled manner evaluate the participation of each element on the obesity and insulin resistance and provide information for better understanding the physiopathology and treatment of obesity and insulin resistance. Experimental obesity and insulin resistance can be due to a deficient response to leptin, secondary to hypoleptinemia and/or mutations on leptin receptor, by modifications on insulin receptor, deletion or diminished insulin signal transduction, enhancement of the effects of orexigen peptides and/or diminution of anorexigen peptides actions on hypothalamus, as well as secondary to arterial hypertension, as in the spontaneously hypertension...
April 2006: Arquivos Brasileiros de Endocrinologia e Metabologia
https://www.readbyqxmd.com/read/11334372/rates-of-gluconeogenesis-in-perfused-liver-of-alloxan-diabetic-fed-rats
#4
COMPARATIVE STUDY
L S Akimoto, S R Pedrinho, G Lopes, R B Bazotte
The maximal capacity of the liver to produce glucose was examined using a technique, which permited to investigate gluconeogenesis in diabetic fed rats. Diabetes was obtained with an iv injection of alloxan (40 mg/kg). Livers from diabetic fed rats were perfused in situ and gluconeogenesis from different glucose precursors were measured one week after alloxan injection. Hepatic gluconeogenesis from L-alanine (5 mM) was absent. However, increased urea, pyruvate and L-lactate production was observed during L-alanine infusion...
2000: Research Communications in Molecular Pathology and Pharmacology
https://www.readbyqxmd.com/read/9209707/glucose-dependent-transcriptional-regulation-of-the-human-sucrase-isomaltase-si-gene
#5
A Rodolosse, V Carrière, I Chantret, M Lacasa, A Zweibaum, M Rousset
We have previously shown that the transcription of the human sucrase-isomaltase (SI) gene was negatively regulated by glucose. Using two clonal metabolic variants of the human colon adenocarcinoma cell line Caco-2 we demonstrate here that: 1) although similar growth-related variations of phosphoenolpyruvate carboxykinase (PEPCK), frutose 1,6-diphosphatase (F1, 6-dPase), pyruvate kinase (PK) and SI mRNA levels are observed, only F1,6-dPase, PK and SI mRNA levels vary in the same way in response to modifications of glucose utilization; and 2) regulatory elements responsible for the glucose-dependent transcription of the SI gene are located within the -370/+30 region of the promoter...
February 1997: Biochimie
https://www.readbyqxmd.com/read/1184182/-the-effect-of-halothane-on-the-fructose-metabolism-in-the-liver
#6
E Götz, R Scholz
Glucose production from frutose (2 mmol) and fructolysis was studied in perfused rat liver. In the presence of halothane (0.5, 1.5, and 4.0 vol%) glucose production was inhibited, whereas lactate production was stimulated. Total fructose metabolism was unchanged. Since halogenated hydrocarbon compounds are known to inhibit the mitochondrial respiratory chain, it is concluded that glucose synthesis is inhibited due to decreased supply of energy-rich phosphates from oxidative phosphorylation. On the other hand, this depletion of energy may be partially compensated for by an increased extramitochondrial energy production due to fructolysis...
October 1975: Infusionstherapie und Klinische Ernährung
https://www.readbyqxmd.com/read/1184179/-the-importance-of-the-carbohydrate-portion-in-a-synthetic-diet
#7
H Förster, G Heller, H P Fortmeyer
The influence of an almost fat-free and carbohydrate-rich diet on different serum parameters was investigated in human volunteers. These studies were supplemented by animal experiments where the effects of different additions of carbohydrates were compared. In human volunteers the fat-free and carbohydrate-rich diet caused a decrease in serum cholesterol concentration. This effect was especially seen when the initial cholesterol values were elevated. Exchange of the glucose carbohydrates (consisting predominantly of oligosaccharides) by frutose or by surose was without an influence on serum concentrations of cholesterol or triglycerides...
October 1975: Infusionstherapie und Klinische Ernährung
https://www.readbyqxmd.com/read/868432/-pathogenesis-of-chronic-alcohosim
#8
A M Danilenko
The paper is concerned with a study of the enzyme activity which reflects the functional state of the liver in 123 patients with chronic alcoholism (without clinical signs of disturbed liver function). The blood serum was studied for sorbitdehydrogenase, frutose-I-phosphataldolase, glutamino-asparagine and glutamine-alanine transamine. It was established that there is an increase in the activity of specific and nonspecific liver enzymes. This increase was in a direct correlation with the clinical expression and severity of the disease (withdrawal syndrome, delirious condition)...
1977: Zhurnal Nevropatologii i Psikhiatrii Imeni S.S. Korsakova
https://www.readbyqxmd.com/read/743202/purification-and-properties-of-the-native-form-of-rabbit-liver-aldolase-evidence-for-proteolytic-modification-after-tissue-extraction
#9
A Chappel, N J Hoogenraad, R S Holmes
Aldolase was purified from rabbit liver by affinity-elution chromatography. By taking precautions to avoid rupture of lysosomes during the isolation procedure, a stable form of liver aldolase was obtained. The stable form of the enzyme had a specific activity with respect to fructose 1,6-bisphosphate cleavage of 20-28 mumol/min per mg of protein and a fructose 1,6-bisphosphate cleavage of 20-28mumol/min per mg of protein and a frutose 1,6-bisphosphate/fructose 1-phosphate activity ratio of 4. It was distinguishable from rabbit muscle aldolase, as previously isolated, on the basis of its electrophoretic mobility and N-terminal analysis...
November 1, 1978: Biochemical Journal
https://www.readbyqxmd.com/read/686176/effects-of-renal-fuels-on-uptake-of-pah-and-uric-acid-by-separated-renal-tubules-of-the-rabbit
#10
I Kippen, J R Klinenberg
A rapid-filtration procedure was used to examine the effects of a wide variety of renal fuels on the uptake of p-aminohippuric acid (PAH) and uric acid (UA) by separated rabbit renal tubules. PAH and UA uptakes in 15 min over a range of substrate concentrations of 0.01-10.2 mM were determined. All tricarboxylic acid cycle intermediates and pyruvate showed biphasic stimulation of PAH and UA uptake. alpha-Ketoglutarate produced a 320 +/- 54% increase in PAH uptake and a 192 +/- 60% increase in UA uptake at 0...
August 1978: American Journal of Physiology
https://www.readbyqxmd.com/read/193561/substrate-form-of-d-frutose-1-6-bisphosphate-utilized-by-fructose-1-6-bisphosphatase
#11
W A Frey, R Fishbein, M M de Maine, S J Benkovic
Rapid quench kinetic experiments on fructose 1,6-bisphosphatase demonstrate a stereospecificity for the alpha anomer of fructose 1,6-bisphosphate relative to the beta configuration. The beta anomer is only utilized after mutarotation to the alpha form in a process that is not enzyme catalyzed. Studies employing analogues of the acyclic keto configuration indicate that the keto form is utilized at a rate less than 5% that of the alpha anomer, a finding also confirmed by computer simulation of the rapid quench data...
May 31, 1977: Biochemistry
https://www.readbyqxmd.com/read/172281/interaction-of-ethacrynic-acid-with-control-sites-of-renal-glucose-metabolism
#12
G Fúlgraff, H Dingler-Núnemann
Ethacrynic acid stimulates in vitro concentration dependent renal gluconeogenesis from substrates which enter the gluconeogenic pathway at the level of the triosephosphates like glycerol or fructose or from substrates which have to pass the oxaloacetate shuttle like pyruvate or from intermediary products of fatty acid oxydation or citrate cycle. Our results suggest that a site of action of ethacrynic acid in this metabolic aspect is the enzyme system fructose diphosphatase/frutose-6-phosphate kinase and eventually additionally pyruvate carboxylase...
1975: Current Problems in Clinical Biochemistry
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