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Leonardo Romorini, Ximena Garate, Gabriel Neiman, Carlos Luzzani, Verónica Alejandra Furmento, Alejandra Sonia Guberman, Gustavo Emilio Sevlever, María Elida Scassa, Santiago Gabriel Miriuka
Human embryonic and induced pluripotent stem cells are self-renewing pluripotent stem cells (PSC) that can differentiate into a wide range of specialized cells. Basic fibroblast growth factor is essential for PSC survival, stemness and self-renewal. PI3K/AKT pathway regulates cell viability and apoptosis in many cell types. Although it has been demonstrated that PI3K/AKT activation by bFGF is relevant for PSC stemness maintenance its role on PSC survival remains elusive. In this study we explored the molecular mechanisms involved in the regulation of PSC survival by AKT...
October 20, 2016: Scientific Reports
María Questa, Leonardo Romorini, Carolina Blüguermann, Claudia María Solari, Gabriel Neiman, Carlos Luzzani, María Élida Scassa, Gustavo Emilio Sevlever, Alejandra Sonia Guberman, Santiago Gabriel Miriuka
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.
March 2016: Stem Cell Research
Annette Leibing, Nancy Guberman, Janine Wiles
There are many studies that have examined the meaning of home for older people. In this article, our aim is to add the concept of 'liminal homes' to the existing discussion: While the concept of liminal homes can be applied to a number of 'interim spaces', we focus in our study, on those older people who have to consider, or are concretely confronted with, the need to move into another living space, because of declining health. Based on interviews and photo-elicitation with 26 older lower-income seniors living in Montreal, Québec, this article demonstrates the complexity of liminality and analyzes the dynamics of this process, composed of a web of interrelated and often dichotomous elements...
April 2016: Journal of Aging Studies
Claudia Solari, Camila Vázquez Echegaray, Carlos Luzzani, María Soledad Cosentino, Ariel Waisman, María Victoria Petrone, Marcos Francia, Alina Sassone, Jésica Canizo, Gustavo Sevlever, Lino Barañao, Santiago Miriuka, Alejandra Guberman
Addition of methyl groups to arginine residues is catalyzed by a group of enzymes called Protein Arginine Methyltransferases (Prmt). Although Prmt1 is essential in development, its paralogue Prmt8 has been poorly studied. This gene was reported to be expressed in nervous system and involved in neurogenesis. In this work, we found that Prmt8 is expressed in mouse embryonic stem cells (ESC) and in induced pluripotent stem cells, and modulated along differentiation to neural precursor cells. We found that Prmt8 promoter activity is induced by the pluripotency transcription factors Oct4, Sox2 and Nanog...
April 22, 2016: Biochemical and Biophysical Research Communications
Laura Mc Conti, Tatiana Champion, Úrsula C Guberman, Carlos Ht Mathias, Stéfano L Fernandes, Elisângela Gm Silva, Monique A Lázaro, Aline Dcg Lopes, Viviane R Fortunato
OBJECTIVES: This study assessed behavioral and physiologic stress parameters in cats placed in two environments: home and the veterinary hospital. With a widely used scale, several parameters were assessed, including respiratory rate (RR), heart rate (HR), systolic blood pressure (SBP), vagosympathetic responses using calculated intervals (heart rate variability [HRV]10, HRV20 and vasovagal tonus index [VVTI]) and 'stress attitude', such as struggling, vocalization and agitation during handling...
December 10, 2015: Journal of Feline Medicine and Surgery
Claudia Solari, Camila Vázquez Echegaray, María Soledad Cosentino, María Victoria Petrone, Ariel Waisman, Carlos Luzzani, Marcos Francia, Emilly Villodre, Guido Lenz, Santiago Miriuka, Lino Barañao, Alejandra Guberman
Pluripotent stem cells possess complex systems that protect them from oxidative stress and ensure genomic stability, vital for their role in development. Even though it has been reported that antioxidant activity diminishes along stem cell differentiation, little is known about the transcriptional regulation of the involved genes. The reported modulation of some of these genes led us to hypothesize that some of them could be regulated by the transcription factors critical for self-renewal and pluripotency in embryonic stem cells (ESCs) and in induced pluripotent stem cells (iPSCs)...
2015: PloS One
Samuel Lapalme-Remis, Evan Cole Lewis, Christine De Meulemeester, Pranesh Chakraborty, K Michael Gibson, Carlos Torres, Alan Guberman, Gajja S Salomons, Cornelis Jakobs, Andre Ali-Ridha, Mahsa Parviz, Phillip L Pearl
OBJECTIVE: The natural history of succinic semialdehyde dehydrogenase (SSADH) deficiency in adulthood is unknown; we elucidate the clinical manifestations of the disease later in life. METHODS: A 63-year-old man with long-standing intellectual disability was diagnosed with SSADH deficiency following hospitalization for progressive decline, escalating seizures, and prolonged periods of altered consciousness. We present a detailed review of his clinical course and reviewed our SSADH deficiency database adult cohort to derive natural history information...
September 8, 2015: Neurology
Carlos Luzzani, Gabriel Neiman, Ximena Garate, María Questa, Claudia Solari, Darío Fernandez Espinosa, Marcela García, Ana Lía Errecalde, Alejandra Guberman, María Elida Scassa, Gustavo Emilio Sevlever, Leonardo Romorini, Santiago Gabriel Miriuka
INTRODUCTION: Mesenchymal stem cells (MSCs) are a promising source of cells for regenerative therapies. Although they can be isolated easily from several tissues, cell expansion is limited since their properties are lost with successive passages. Hence, pluripotent derived MSCs (PD-MSCs) arise as a suitable alternative for MSC production. Nevertheless, at present, PD-MSC derivation protocols are either expensive or not suitable for clinical purposes. METHODS: In this work we present a therapy-grade, inexpensive and simple protocol to derive MSCs from pluripotent stem cells (PSCs) based on the use of platelet lysate (PL) as medium supplement...
2015: Stem Cell Research & Therapy
Steven L Guberman
Dissociative recombination rate constants are reported with electron temperature dependent uncertainties for the lowest 5 vibrational levels of the N2 (+) ground state. The rate constants are determined from ab initio calculations of potential curves, electronic widths, quantum defects, and cross sections. At 100 K electron temperature, the rate constants overlap with the exception of the third vibrational level. At and above 300 K, the rate constants for excited vibrational levels are significantly smaller than that for the ground level...
November 28, 2014: Journal of Chemical Physics
Haya Brama, Shoshana Guberman, Moshe Abeles, Edward Stern, Ido Kanter
Periodic synchronization of activity among neuronal pools has been related to substantial neural processes and information throughput in the neocortical network. However, the mechanisms of generating such periodic synchronization among distributed pools of neurons remain unclear. We hypothesize that to a large extent there is interplay between the topology of the neocortical networks and their reverberating modes of activity. The firing synchronization is governed by a nonlocal mechanism, the network delay loops, such that distant neuronal pools without common drives can be synchronized...
November 2015: Brain Structure & Function
Amir Goldental, Shoshana Guberman, Roni Vardi, Ido Kanter
In 1943 McCulloch and Pitts suggested that the brain is composed of reliable logic-gates similar to the logic at the core of today's computers. This framework had a limited impact on neuroscience, since neurons exhibit far richer dynamics. Here we propose a new experimentally corroborated paradigm in which the truth tables of the brain's logic-gates are time dependent, i.e., dynamic logic-gates (DLGs). The truth tables of the DLGs depend on the history of their activity and the stimulation frequencies of their input neurons...
2014: Frontiers in Computational Neuroscience
Daniel Pollack, Greg Khaimov, Ronald Guberman
The purpose of this case study was to highlight a potential limitation of magnetic resonance imaging in diagnosing longitudinal tendon tears and to emphasize the importance of clinical examination for peroneal tendinopathy. We describe a 15-year-old female with lateral ankle pain, who was negative for peroneal tendon tear on magnetic resonance imaging. Owing to high clinical suspicion of peroneal tendon pathology, we opted to take the patient to the operating room and found a 6.5-cm longitudinal tear and a low-lying muscle belly of the peroneus brevis tendon...
January 2014: Journal of the American Podiatric Medical Association
Timothy Zeko, Steven F Hannigan, Timothy Jacisin, Matthew J Guberman-Pfeffer, Eric R Falcone, Melissa J Guildford, Christopher Szabo, Kathryn E Cole, Jessica Placido, Erin Daly, Matthew A Kubasik
Isotope-edited FT-IR spectroscopy is a combined synthetic and spectroscopic method used to characterize local (e.g., residue-level) vibrational environments of biomolecules. We have prepared the 3(10) helical peptide Z-Aib6-OtBu and seven (13)C-enriched analogues that vary only in the number and position(s) of (13)C═O isotopic enrichment. FT-IR spectra of these eight peptides solvated in the nonpolar aprotic solvent dichloromethane have been collected and compared to frequency, intensity, and normal mode results of DFT calculations...
January 9, 2014: Journal of Physical Chemistry. B
I Reidler, M Nixon, Y Aviad, S Guberman, A A Friesem, M Rosenbluh, N Davidson, I Kanter
The synchronization of chaotic lasers and the optical phase synchronization of light originating in multiple coupled lasers have both been extensively studied. However, the interplay between these two phenomena, especially at the network level, is unexplored. Here, we experimentally compare these phenomena by controlling the heterogeneity of the coupling delay times of two lasers. While chaotic lasers exhibit deterioration in synchronization as the time delay heterogeneity increases, phase synchronization is found to be independent of heterogeneity...
October 15, 2013: Optics Letters
Noelia Losino, Ariel Waisman, Claudia Solari, Carlos Luzzani, Darío Fernández Espinosa, Alina Sassone, Andrés F Muro, Santiago Miriuka, Gustavo Sevlever, Lino Barañao, Alejandra Guberman
Embryonic stem cells (ESC) need a set of specific factors to be propagated. They can also grow in conditioned medium (CM) derived from a bovine granulosa cell line BGC (BGC-CM), a medium that not only preserves their main features but also increases ESC´s proliferation rate. The mitogenic properties of this medium were previously reported, ascribing this effect to an alternative spliced generated fibronectin isoform that contains the extra domain A (FN EDA(+)). Here, we investigated if the FN EDA(+) isoform increased proliferation of mouse and human ES cells...
2013: PloS One
Roni Vardi, Amir Goldental, Shoshana Guberman, Alexander Kalmanovich, Hagar Marmari, Ido Kanter
A classical view of neural coding relies on temporal firing synchrony among functional groups of neurons, however, the underlying mechanism remains an enigma. Here we experimentally demonstrate a mechanism where time-lags among neuronal spiking leap from several tens of milliseconds to nearly zero-lag synchrony. It also allows sudden leaps out of synchrony, hence forming short epochs of synchrony. Our results are based on an experimental procedure where conditioned stimulations were enforced on circuits of neurons embedded within a large-scale network of cortical cells in vitro and are corroborated by simulations of neuronal populations...
2013: Frontiers in Neural Circuits
Steven L Guberman
Theoretical ab initio calculations are reported of the cross sections for dissociative recombination of the lowest four excited vibrational levels of N2(+) at electron energies from 0.001 to 1.0 eV. Rydberg vibrational levels contributing to the cross section structures are identified as are dissociative channels contributing more than 10(-16) cm(2) to the total cross sections. In contrast to the prior study of v = 0 (S. L. Guberman, J. Chem. Phys. 137, 074309 (2012)), which showed 2(3)Πu to be the dominant dissociative channel, 4(3)Πu is dominant for v = 1...
September 28, 2013: Journal of Chemical Physics
Trevor Changgun Lee, Annie Dupuis, Emily Jones, Carly Guberman, Monique Herbert, Katharina Manassis
OBJECTIVE: It remains unclear whether an anxiety diagnosis is associated with children's emotional recognition. We considered children's age and types of primary anxiety diagnosis, which have been neglected, to elucidate this relationship. METHODS: Sixty-three referred children with anxiety disorder(s) and 59 volunteer children without anxiety disorder(s), aged between 6 and 11 years, were presented with animated characters, displaying a range of simple and complex emotions, for identification...
May 2013: Canadian Journal of Psychiatry. Revue Canadienne de Psychiatrie
Cristiane Guberman, Juanita K Jellyman, Guang Han, Michael G Ross, Mina Desai
OBJECTIVE: A maternal high-fat diet creates an increased risk of offspring obesity and systemic hypertension. Although the renal renin-angiotensin system (RAS) is known to regulate blood pressure, it is now recognized that the RAS is also activated in adipose tissue during obesity. We hypothesized that programmed offspring hypertension is associated with the activation of the adipose tissue RAS in the offspring of obese rat dams. STUDY DESIGN: At 3 weeks of age, female rats were weaned to a high-fat diet (60% k/cal; n = 6) or control diet (10% k/cal; n = 6)...
September 2013: American Journal of Obstetrics and Gynecology
Nitsan Maharshak, Shani Shenhar-Tsarfaty, Nimrod Aroyo, Naama Orpaz, Irene Guberman, Jonathan Canaani, Zamir Halpern, Iris Dotan, Shlomo Berliner, Hermona Soreq
BACKGROUND: MicroRNA-132 (miR-132) targets acetylcholinesterase (AChE) and potentiates the cholinergic blockade of inflammatory reactions in cultured cells and experimental mice, but the implications of this interaction to human inflammatory disease remained unexplored. This study aimed to test whether miR-132 is causally involved in anti-inflammatory reactions of patients with inflammatory bowel disease (IBD) and modulates vagal tone and consequently inflammation in patients with IBD...
June 2013: Inflammatory Bowel Diseases
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