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miRNA,long non-coding RNA,

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https://www.readbyqxmd.com/read/28803540/preparation-of-highly-multiplexed-small-rna-sequencing-libraries
#1
Helena Persson, Rolf Søkilde, Anna Chiara Pirona, Carlos Rovira
MicroRNAs (miRNAs) are ~22-nucleotide-long small non-coding RNAs that regulate the expression of protein-coding genes by base pairing to partially complementary target sites, preferentially located in the 3´ untranslated region (UTR) of target mRNAs. The expression and function of miRNAs have been extensively studied in human disease, as well as the possibility of using these molecules as biomarkers for prognostication and treatment guidance. To identify and validate miRNAs as biomarkers, their expression must be screened in large collections of patient samples...
August 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/28799568/association-of-long-non-coding-rna-h19-and-microrna-21-expression-with-the-biological-features-and-prognosis-of-non-small-cell-lung-cancer
#2
Y Zhou, B Sheng, Q Xia, X Guan, Y Zhang
In recent years, long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have been shown to play important roles in tumor biological function. The aim of this study was to investigate the diagnostic and prognostic value of lncRNA H19 and miR-21 expression in non-small-cell lung cancer (NSCLC). H19 and miR-21 expression was measured in tumor tissues and corresponding non-tumor lung tissues from 200 patients by quantitative reverse transcription polymerase chain reaction. Moreover, the in vitro and in vivo effects of H19 knock out in A549 cells were investigated...
August 11, 2017: Cancer Gene Therapy
https://www.readbyqxmd.com/read/28776911/long-non-coding-rnas-as-an-epigenetic-regulator-in-human-cancers
#3
REVIEW
Yutaka Kondo, Keiko Shinjo, Keisuke Katsushima
Recent studies have demonstrated the important multiple roles of long non-coding RNAs (lncRNAs) during oncogenic transformation. Because the coding genome accounts for a small amount of total DNA, and many mutations leading to cancer occur in the non-coding genome, it is plausible that the dysregulation of such non-coding transcribes might also affect tumor phenotypes. Indeed, to date, lncRNAs have been reported to affect diverse biological processes through the regulation of mRNA stability, RNA splicing, chromatin structure, and miRNA-mediated gene regulation by acting as miRNA sponges...
August 4, 2017: Cancer Science
https://www.readbyqxmd.com/read/28763747/long-intergenic-noncoding-rna-00305-sponges-mir-136-to-regulate-the-hypoxia-induced-apoptosis-of-vascular-endothelial-cells
#4
Bo-Ya Zhang, Zhe Jin, Zhuo Zhao
BACKGROUND: Apoptosis in vascular endothelial cells (VECs) are closely correlated to multiple endotheliocyte-related cardiovascular diseases, for example atherosclerosis. Long non-coding RNAs (lncRNAs) have been testified to play important role in regulation of VECs. The purpose of this study is to investigate the potential regulation of lncRNA long intergenic noncoding RNA 00305 (LINC00305) on hypoxia induced VECs. METHODS: Human umbilical vein endothelial cells (HUVECs) were respectively induced with normoxia or hypoxia (1%)...
July 29, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28744190/hypoxia-in-cns-pathologies-emerging-role-of-mirna-based-neurotherapeutics-and-yoga-based-alternative-therapies
#5
REVIEW
Gillipsie Minhas, Deepali Mathur, Balakrishnan Ragavendrasamy, Neel K Sharma, Viraaj Paanu, Akshay Anand
Cellular respiration is a vital process for the existence of life. Any condition that results in deprivation of oxygen (also termed as hypoxia) may eventually lead to deleterious effects on the functioning of tissues. Brain being the highest consumer of oxygen is prone to increased risk of hypoxia-induced neurological insults. This in turn has been associated with many diseases of central nervous system (CNS) such as stroke, Alzheimer's, encephalopathy etc. Although several studies have investigated the pathophysiological mechanisms underlying ischemic/hypoxic CNS diseases, the knowledge about protective therapeutic strategies to ameliorate the affected neuronal cells is meager...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28738776/graph-based-clustering-of-mirna-sequences
#6
Viviani A Kasahara, Maria do Carmo Nicoletti
MicroRNAs (miRNAs) are approximately 22 nucleotide-long non-coding RNA molecules, that play important roles in gene regulation. They regulate several processes, such as development, metabolism and cell death, among many others. The many miRNAs discovered so far have been divided into biologically representative families, aiming at organizing and systematizing their study so to promote, mainly, a better understanding of their functionalities. Considering that members of the same miRNA family tend to biologically function in similar ways, a well-structured family can help detecting miRNA functions which have not been associated yet with any existing family...
July 24, 2017: MicroRNA
https://www.readbyqxmd.com/read/28729919/toward-understanding-non-coding-rna-roles-in-intracranial-aneurysms-and-subarachnoid-hemorrhage
#7
Fengzhen Huang, Jiping Yi, Tieqiao Zhou, Xiaoxiang Gong, Hong Jiang, Xiaoxi Yao
Subarachnoid hemorrhage (SAH) is a common and frequently life-threatening cerebrovascular disease, which is mostly related with a ruptured intracranial aneurysm. Its complications include rebleeding, early brain injury, cerebral vasospasm, delayed cerebral ischemia, chronic hydrocephalus, and also non neurological problems. Non-coding RNAs (ncRNAs), comprising of microRNAs (miRNAs), small interfering RNAs (siRNAs) and long non-coding RNAs (lncRNAs), play an important role in intracranial aneurysms and SAH. Here, we review the non-coding RNAs expression profile and their related mechanisms in intracranial aneurysms and SAH...
2017: Translational Neuroscience
https://www.readbyqxmd.com/read/28728223/-expression-and-bioinformatics-analysis-of-long-chain-non-coding-rna-pvt1-in-tumors
#8
M Wang, W Gao, Y F Bai, D H Lu, L H Teng
Objective: To study the expression and mechanism of long-chain non-coding RNA PVT1 in tumor by bioinformatics analysis and experimental verification, and to provide new ideas for the study of the pathogenesis of tumors. Methods: The expression of PVT1 in 14 common tumors was downloaded from starBase v2.0 public database, which also was verified by PVT1 RNA-in situ hybridization.The upstream transcription factors, the downstream target microRNA(miRNA) for PVT1 and the target genes for the target miRNAs were predicted and analyzed by using bioinformatics based on the database of UCSC Genome Browser, HMDD v2...
July 8, 2017: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/28724410/identification-and-characterization-of-long-non-coding-rnas-in-subcutaneous-adipose-tissue-from-castrated-and-intact-full-sib-pair-huainan-male-pigs
#9
Jing Wang, Liushuai Hua, Junfeng Chen, Jiaqing Zhang, Xianxiao Bai, Binwen Gao, Congjun Li, Zhihai Shi, Weidong Sheng, Yuan Gao, Baosong Xing
BACKGROUND: Long non-coding RNAs (lncRNAs) regulate adipose tissue metabolism, however, their function on testosterone deficiency related obesity in humans is less understood. For this research, intact and castrated male pigs are the best model animal because of their similar proportional organ sizes, cardiovascular systems and metabolic features. RESULTS: We identified lncRNAs in subcutaneous adipose tissue by deep RNA-sequencing using the intact and castrated Huainan male pigs...
July 19, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28722665/mirna-and-lncrna-as-biomarkers-in-cholangiocarcinoma-cca
#10
REVIEW
Bo Zheng, Seogsong Jeong, Yanjing Zhu, Lei Chen, Qiang Xia
The microRNAs are a group of 20 nucleotides-long non-coding RNAs. By binding to the 3'UTR region of target mRNA, microRNAs can perform extensive actions mediating gene expression at post-trancriptional stages. It makes microRNAs serve as very crucial regulators in various biological progress including carcinogenesis. Long non-coding RNAs, however, are a subgroup of RNA with the length of 200 nucleotides. Unlike microRNAs, long non-coding RNAs can form secondary of tertiary domain based on their length. With the ability of directly interacting with DNA, RNA, protein, long non-coding RNAs have promoting or inhibitive functions in gene expression regulation...
July 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28719033/identification-of-driver-copy-number-alterations-in-diverse-cancer-types-and-application-in-drug-repositioning
#11
Wenbin Zhou, Zhangxiang Zhao, Ruiping Wang, Yue Han, Chengyu Wang, Fan Yang, Ya Han, Haihai Liang, Lishuang Qi, Chenguang Wang, Zheng Guo, Yunyan Gu
Results from numerous studies suggest an important role for somatic copy number alterations (SCNAs) in cancer progression. Our work aimed to identify the drivers (oncogenes or tumor suppressor genes) that reside in recurrently aberrant genomic regions, including a large number of genes or non-coding genes, which remain a challenge for decoding the SCNAs involved in carcinogenesis. Here, we propose a new approach to comprehensively identify drivers, using 8740 cancer samples involving 18 cancer types from The Cancer Genome Atlas (TCGA)...
July 18, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/28716020/long-non-coding-rna-casc2-suppresses-epithelial-mesenchymal-transition-of-hepatocellular-carcinoma-cells-through-casc2-mir-367-fbxw7-axis
#12
Yufeng Wang, Zhikui Liu, Bowen Yao, Qing Li, Liang Wang, Cong Wang, Changwei Dou, Meng Xu, Qingguang Liu, Kangsheng Tu
BACKGROUND: Recently, it has been reported that long non-coding RNA (lncRNA) cancer susceptibility candidate 2 (CASC2), a novel tumor suppressor, participates in regulating the carcinogenesis and suppresses tumor progression by sponging microRNAs (miRNAs). However, the expression and function of CASC2 in hepatocellular carcinoma (HCC) remain unclear. METHODS: The expression of CASC2 and miR-367 in HCC specimens and cell lines were detected by real-time PCR. Western blotting and immunohistochemistry were carried out for detection of epithelial-to-mesenchymal transition (EMT) markers in HCC...
July 17, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28715488/microrna-dependent-and-independent-deregulation-of-long-non-coding-rnas-by-an-oncogenic-herpesvirus
#13
Sunantha Sethuraman, Lauren Appleby Gay, Vaibhav Jain, Irina Haecker, Rolf Renne
Kaposi's sarcoma (KS) is a highly prevalent cancer in AIDS patients, especially in sub-Saharan Africa. Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of KS and other cancers like Primary Effusion Lymphoma (PEL). In KS and PEL, all tumors harbor latent KSHV episomes and express latency-associated viral proteins and microRNAs (miRNAs). The exact molecular mechanisms by which latent KSHV drives tumorigenesis are not completely understood. Recent developments have highlighted the importance of aberrant long non-coding RNA (lncRNA) expression in cancer...
July 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28713971/systematic-analysis-of-lncrnas-mirnas-and-mrnas-for-the-identification-of-biomarkers-for-osteoporosis-in-the-mandible-of-ovariectomized-mice
#14
Lingyu Hao, Jiayao Fu, Yawen Tian, Junhua Wu
Osteoporosis is a complex and multifactorial disease caused by an imbalance between bone formation and resorption. Post‑menopausal women with endogenous estrogen deficiency suffer from systemic bone loss and osteoporosis, and are at high risk of this affecting the jaw bones. MicroRNAs (miRNAs or miRs) have been implicated in the mechanisms of metabolic bone diseases and are expressed at differential levels in alveolar bone following ovariectomy. In the present study, we systematically analyzed the expression profiles of miRNAs, mRNAs and long non‑coding RNA (lncRNAs) in the mandible of ovariectomized (OVX) mice...
September 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28713968/inhibition-of-proliferation-and-invasion-of-hepatocellular-carcinoma-cells-by-lncrna-aslnc02525-silencing-and-the-mechanism
#15
Zi Chen, Dongwen Xu, Tao Zhang
One of the most differentially expressed long non-coding RNAs (lncRNAs) that we identified by high throughput screening from liver cancer and para-carcinoma tissues, ASLNC02525, was highly expressed in the tissues and cell lines of liver cancer but not in adjacent tissues or normal hepatic cells. Knockdown of ASLNC02525 in hepatocellular carcinoma cells inhibited the proliferation and invasion. In the process, expression level of transcription factor twist1 (twist‑related protein 1) was reduced, but no change at transcription level was observed...
July 7, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28703782/understanding-the-role-of-non-coding-rnas-in-bladder-cancer-from-dark-matter-to-valuable-therapeutic-targets
#16
REVIEW
Cecilia Pop-Bica, Diana Gulei, Roxana Cojocneanu-Petric, Cornelia Braicu, Bogdan Petrut, Ioana Berindan-Neagoe
The mortality and morbidity that characterize bladder cancer compel this malignancy into the category of hot topics in terms of biomolecular research. Therefore, a better knowledge of the specific molecular mechanisms that underlie the development and progression of bladder cancer is demanded. Tumor heterogeneity among patients with similar diagnosis, as well as intratumor heterogeneity, generates difficulties in terms of targeted therapy. Furthermore, late diagnosis represents an ongoing issue, significantly reducing the response to therapy and, inevitably, the overall survival...
July 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28699365/activating-the-chromatin-by-noncoding-rnas
#17
Nihay Laham-Karam, Pia Laitinen, Tiia A Turunen, Seppo Ylä-Herttuala
SIGNIFICANCE: The extent and breadth of transcription have recently been uncovered and this has revealed an extensive array of noncoding RNAs (ncRNAs). The biological role and significance of these ncRNAs have been realized and to date it appears that ncRNAs may have many important regulatory functions. ncRNAs are multifaceted and they induce a complexity of different types of transcriptional and posttranscriptional regulation, including gene activation. Recent Advances: Association of ncRNAs with gene activation is an important finding...
August 10, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28697764/long-non-coding-rna-linc00673-regulated-non-small-cell-lung-cancer-proliferation-migration-invasion-and-epithelial-mesenchymal-transition-by-sponging-mir-150-5p
#18
Wei Lu, Honghe Zhang, Yuequn Niu, Yongfeng Wu, Wenjie Sun, Hongyi Li, Jianlu Kong, Kefeng Ding, Han-Ming Shen, Han Wu, Dajing Xia, Yihua Wu
BACKGROUND: The function of a new long non-coding RNA linc00673 remains unclear. While identified as an oncogenic player in non-small cell lung cancer (NSCLC), linc00673 was found to be anti-oncogenic in pancreatic ductal adenocarcinoma (PDAC). However whether linc00673 regulated malignancy and epithelial mesenchymal transition (EMT) has not been characterized. METHODS: Cell proliferation was assessed using CCK-8 and EdU assays, and cell migration and invasion were assessed using scratch assays and transwell invasion assays...
July 11, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28672765/discovery-and-annotation-of-plant-endogenous-target-mimicry-sequences-from-public-transcriptome-libraries-a-case-study-of-prunus-persica
#19
Gökhan Karakülah
Novel transcript discovery through RNA sequencing has substantially improved our understanding of the transcriptome dynamics of biological systems. Endogenous target mimicry (eTM) transcripts, a novel class of regulatory molecules, bind to their target microRNAs (miRNAs) by base pairing and block their biological activity. The objective of this study was to provide a computational analysis framework for the prediction of putative eTM sequences in plants, and as an example, to discover previously un-annotated eTMs in Prunus persica (peach) transcriptome...
June 28, 2017: Journal of Integrative Bioinformatics
https://www.readbyqxmd.com/read/28666797/gas5-suppresses-malignancy-of-human-glioma-stem-cells-via-a-mir-196a-5p-foxo1-feedback-loop
#20
Xihe Zhao, Yunhui Liu, Jian Zheng, Xiaobai Liu, Jiajia Chen, Libo Liu, Ping Wang, Yixue Xue
Glioma stem cells (GSCs) make up highly tumorigenic subpopulations within gliomas, and aberrant expression of GSC genes is a major underlying cause of glioma pathogenesis and treatment failure. The present study characterized the expression and function of long non-coding RNA growth arrest specific 5 (GAS5) in GSCs in order to elucidate the molecular mechanisms by which GAS5 contributes to glioma pathogenesis. We demonstrate that GAS5 suppresses GSC malignancy by binding to miR-196a-5p. miR-196a-5p, an onco-miRNA, stimulates GSC proliferation, migration, and invasion, in addition to reducing levels of apoptosis...
June 27, 2017: Biochimica et Biophysica Acta
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