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https://www.readbyqxmd.com/read/28827657/pre-culture-sudan-black-b-treatment-suppresses-autofluorescence-signals-emitted%C3%A2-from-polymer-tissue-scaffolds
#1
Lin Qi, Erin K Knapton, Xu Zhang, Tongwen Zhang, Chen Gu, Yi Zhao
In tissue engineering, autofluorescence of polymer scaffolds often lowers the image contrast, making it difficult to examine cells and subcellular structures. Treating the scaffold materials with Sudan Black B (SBB) after cell fixation can effectively suppress autofluorescence, but this approach is not conducive to live cell imaging. Post-culture SBB treatment also disrupts intracellular structures and leads to reduced fluorescence intensity of the targets of interest. In this study, we introduce pre-culture SBB treatment to suppress autofluorescence, where SBB is applied to polymeric scaffold materials before cell seeding...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28823049/exploring-the-challenges-for-clinical-pharmacists-in-sudan
#2
Hwaida Elsadig, Marjorie Weiss, Jenny Scott, Raisa Laaksonen
Background Clinical pharmacy practice in hospitals is a new role for pharmacists in Sudan. Pharmacists have to face the challenge of moving from their traditional roles within the pharmacy premises to new roles on the wards with direct contact with patients and other healthcare professionals. Objectives To explore the role and challenges facing the clinical pharmacists of Sudan. Settings Two of the main government hospitals in Sudan and an online survey. Method This study applied a two phase mixed method, a focus group discussion and a survey...
August 19, 2017: International Journal of Clinical Pharmacy
https://www.readbyqxmd.com/read/28822936/influence-of-coupled-ocean-atmosphere-phenomena-on-the-greater-horn-of-africa-droughts-and-their-implications
#3
Freddie Mpelasoka, Joseph L Awange, Ayalsew Zerihun
Drought-like humanitarian crises in the Greater Horn of Africa (GHA) are increasing despite recent progress in drought monitoring and prediction efforts. Notwithstanding these efforts, there remain challenges stemming from uncertainty in drought prediction, and the inflexibility and limited buffering capacity of the recurrent impacted systems. The complexity of the interactions of ENSO, IOD, IPO and NAO, arguably remains the main source of uncertainty in drought prediction. To develop practical drought risk parameters that potentially can guide investment strategies and risk-informed planning, this study quantifies, drought characteristics that underpin drought impacts management...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822816/genetically-modified-rabies-virus-vectored-ebola-virus-disease-vaccines-are-safe-and-induce-efficacious-immune-responses-in-mice-and-dogs
#4
Lei Shuai, Xijun Wang, Zhiyuan Wen, Jinying Ge, Jinliang Wang, Dandan Zhao, Zhigao Bu
Ebola viruses (EBOVs) are zoonotic pathogens that cause EBOV disease (EVD) with high case fatality in humans. Currently, EVD vaccines are still under development in several countries. Here, we generated two recombinant rabies viruses (RABVs), rERAG333E/ZGP and rERAG333E/SGP, expressing the Zaire EBOV glycoprotein (ZGP) or Sudan EBOV glycoprotein (SGP) gene based on a modified ERA vaccine strain (rERAG333E) vector platform. The recombinant RABVs retained growth properties similar to those of the vector virus in BSR cell culture and efficiently expressed ZGP or SGP...
August 16, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28821501/perinatal-outcomes-following-helping-babies-breathe-training-and-regular-peer-peer-skills-practice-among-village-midwives-in-sudan
#5
Ame Arabi, S A Ibrahim, M Abdel-Rahman, M S Abdalla, S E Ahmed, E P Dempsey, C A Ryan
BACKGROUND: Over 80% of deliveries in Sudan occur in rural areas, attended by village midwives (VMWs). OBJECTIVE: To determine the impact of Helping Babies Breathe training and regular peer-peer skills practice (HBBT(+RPPSP)) on VMW resuscitation practices and outcomes. METHODS: In a prospective community-based intervention study, 71/82 VMWs, reporting to six East Nile rural medical centres, with previous experience in community health research, consented to HBBT(+RPPSP)...
August 18, 2017: Archives of Disease in Childhood
https://www.readbyqxmd.com/read/28819535/clinical-features-and-antinuclear-antibodies-profile-among-adults-with-systemic-lupus-erythematosus-and-lupus-nephritis-a-cross-sectional-study
#6
Nashwa Ahmed, Mazin Shigidi, Al Nour Al Agib, Hassan Abdelrahman, Elshafie Taha
INTRODUCTION: Limited data is available regarding the clinical manifestations and pattern of Systemic Lupus Erythematosus (SLE) in Sudan. This study aimed to determine the clinical manifestations and Antinuclear Antibodies (ANA) profile among Sudanese adults with SLE and lupus nephritis (LN). METHODS: A descriptive study was conducted in Omdurman Military Hospital, Sudan. It included all adults with SLE and on regular follow-up during the study period (December 2012 to May 2013)...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28819192/scalable-fabrication-of-the-graphitic-substrates-for-graphene-enhanced-raman-spectroscopy
#7
Tommi Kaplas, Antti Matikainen, Tarmo Nuutinen, Sari Suvanto, Pasi Vahimaa, Yuri Svirko
We propose direct synthesis of ultra-thin graphitic films on a dielectric substrate using sacrificial Ni catalyst layer, which significantly increases the crystallinity of the photoresist pyrolyzed at the temperature of 800 °C and above. A considerable amount of multilayer graphene in the photoresist film pyrolyzed in the presence of the Ni catalyst gives rise to an enhancement of the Raman signal of dye Sudan III molecules deposited on the substrate. We demonstrate comparable enhancement of the Raman signal from Sudan III molecules deposited on the fabricated graphitic substrate and those deposited on graphene, which was conventionally transferred to the silica substrate...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28817726/evaluation-of-peptide-designing-strategy-against-subunit-reassociation-in-mucin-1-a-steered-molecular-dynamics-approach
#8
J Lesitha Jeeva Kumari, R Jesu Jaya Sudan, C Sudandiradoss
Subunit reassociation in mucin 1, a breast cancer tumor marker, is reported as one of the critical factors for its cytoplasmic activation. Inhibition of its heterodimeric association would therefore result in loss of its function and alter disease progression. The present study aimed at evaluating peptide inhibitor designing strategies that may serve as antagonist against this receptor-ligand alliance. Several peptides and their derivatives were designed based on native residues, subunit interface, hydrogen bonding and secondary structure...
2017: PloS One
https://www.readbyqxmd.com/read/28817155/genetic-variation-of-15-autosomal-strs-in-a-population-sample-of-bedouins-residing-in-the-area-of-the-fourth-nile-cataract-sudan
#9
Andrzej Ossowski, Jaroslaw Piatek, Miroslaw Parafiniuk, Aleksandra Pudlo, Witold Pepinski, Malgorzata Skawronska, Michal Szeremeta, Anna Niemcunowicz-Janica, Ireneusz Soltyszewski
The purpose of the paper was to report allelic frequencies of 15 autosomal STR markers (AmpFlSTR NGM PCR Amplification Kit) for Bedouin inhabitants in the area of the Fourth Nile Cataract in Sudan, and compute commonly used population and forensic biostatistical parameters. Buccal swabs were collected from 117 unrelated individuals. DNA was extracted using DNA QIAamp(®) DNA Mini Kit, and quantitated with Quantifiler Human Quantification Kit in a 7500 Real-Time PCR System. Amplification of 15 AmpFlSTR NGM PCR Kit loci was performed in PCR System 9700...
August 17, 2017: Anthropologischer Anzeiger; Bericht über die Biologisch-anthropologische Literatur
https://www.readbyqxmd.com/read/28817110/extractive-spectrophotometric-determination-of-nortriptyline-hydrochloride-using-sudan-ii-iv-and-black-b
#10
A S Amin, H M Saleh
A simple spectrophotometric methods has been developed for the determination of nortriptyline hydrochloride in pure and in pharmaceuticalformulations based on the formation of ion-pair complexes with sudun II (SII), sudan (IV) (SIV) and sudan black B (SBB). The selectivity of the method was improved through extraction with chloroform. The optimum conditions for complete extracted colour development were assessed. The absorbance measurements were made at 534, 596 and 649 nm for SII, SIV and SBB complexes, respectively...
August 17, 2017: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/28810668/mass-drug-administration-in-central-equatoria-south-sudan-results-and-suggestions-for-future-distributions
#11
Giuseppina Ortu, Jamshed Khan, Makoy Samuel Yibi, Ismail Abdu Nimaya
Background: South Sudan has rolled out a neglected tropical disease programme, which envisaged deworming campaigns in states endemic for soil transmitted helminth infections and schistosomiasis. Methods: In 2016, two deworming campaigns targeting school-age children were performed in Central Equatoria. Distribution sites were set up in primary schools, Boma Health Initiative headquarters, health centres and markets. Training, radio adverts and community meetings were performed before the campaigns...
July 1, 2017: International Health
https://www.readbyqxmd.com/read/28794012/surgeon-major-thomas-heazle-parke-1857-1893-irish-doctor-soldier-and-explorer
#12
EDITORIAL
Andrew Parke
Surgeon Major Thomas Heazle Parke (1857-1893) was a doctor from Drumsna, County Roscommon, who after completing his education at the Royal College of Surgeons in Ireland joined the British army as a medical officer. After several years of serving in Ireland and Egypt, he volunteered to be medical officer of the Emin Pasha Relief Expedition of 1887-1889. This was to become Henry Morton Stanley's largest, longest and most controversial African expedition. The epic journey saw Stanley, his eight European officers and 800 African porters take almost 3 years to cross the African continent from West to East via the Congo River, Southern Sudan and Uganda...
August 8, 2017: Journal of the Royal Army Medical Corps
https://www.readbyqxmd.com/read/28789834/diabetes-complications-in-sudanese-individuals-with-type-2-diabetes-overlooked-problems-in-sub-saharan-africa
#13
Heitham Awadalla, Sufian K Noor, Wadie M Elmadhoun, Ahmed O Almobarak, Nehad E Elmak, Sulaf I Abdelaziz, Amel A Sulaiman, Mohamed H Ahmed
BACKGROUND: Diabetes mellitus (DM) is a major health problem in Sudan and is a leading cause of morbidity and mortality. The objective of this study is to determine the prevalence of complications of type 2 diabetes across different cities in the country. METHODS: Individuals with type 2 diabetes, who have been on treatment for DM for at least one year and volunteered to participate, were selected from two diabetes centers in Sudan (Khartoum and Atbara). Participants were interviewed using standardized pretested questionnaire to record medical history, socio-demographic, life style characteristics and presence of complications of diabetes...
July 29, 2017: Diabetes & Metabolic Syndrome
https://www.readbyqxmd.com/read/28789627/using-lot-quality-assurance-sampling-to-assess-access-to-water-sanitation-and-hygiene-services-in-a-refugee-camp-setting-in-south-sudan-a-feasibility-study
#14
Elizabeth Harding, Colin Beckworth, Jean-Francois Fesselet, Annick Lenglet, Richard Lako, Joseph J Valadez
BACKGROUND: Humanitarian agencies working in refugee camp settings require rapid assessment methods to measure the needs of the populations they serve. Due to the high level of dependency of refugees, agencies need to carry out these assessments. Lot Quality Assurance Sampling (LQAS) is a method commonly used in development settings to assess populations living in a project catchment area to identify their greatest needs. LQAS could be well suited to serve the needs of refugee populations, but it has rarely been used in humanitarian settings...
August 8, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28780120/vaccines-against-ebola-virus
#15
Navin Venkatraman, Daniel Silman, Pedro M Folegatti, Adrian V S Hill
We have just witnessed the largest and most devastating outbreak of Ebola virus disease, which highlighted the urgent need for development of an efficacious vaccine that could be used to curtail future outbreaks. Prior to 2014, there had been limited impetus worldwide to develop a vaccine since the virus was first discovered in 1976. Though too many lives were lost during this outbreak, it resulted in the significantly accelerated clinical development of a number of candidate vaccines through an extraordinary collaborative global effort coordinated by the World Health Organisation (WHO) and involving a number of companies, trial centres, funders, global stakeholders and agencies...
August 2, 2017: Vaccine
https://www.readbyqxmd.com/read/28774342/crimean-congo-hemorrhagic-fever-among-the-one-humped-camel-camelus-dromedaries-in-central-sudan
#16
Hajer M Suliman, Ibrahim A Adam, Shamseldin I Saeed, Sanaa A Abdelaziz, Eltahir M Haroun, Imadeldin E Aradaib
BACKGROUND: Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral zoonotic disease caused by Crimean-Congo hemorrhagic fever virus (CCHFV), a member of the genus Nairovirus in the family Bunyaviridae. CCHF is typically asymptomatic in animals but can be highly fatal in humans approaching case fatality rate of approximately 30%. In the present investigation, a cross sectional study was conducted to determine the prevalence of CCHF and to identify the potential risk factors associated with CCHFV seropositivity among the one-humped camel (Camelus dromedaries) in Central Sudan...
August 3, 2017: Virology Journal
https://www.readbyqxmd.com/read/28770590/delivering-hiv-care-in-challenging-operating-environments-the-msf-experience-towards-differentiated-models-of-care-for-settings-with-multiple-basic-health-care-needs
#17
REVIEW
Charles Ssonko, Lucia Gonzalez, Anita Mesic, Marcio Silveira da Fonseca, Jay Achar, Nadia Safar, Beatriz Martin, Sidney Wong, Esther C Casas
INTRODUCTION: Countries in the West and Central African regions struggle to offer quality HIV care at scale, despite HIV prevalence being relatively low. In these challenging operating environments, basic health care needs are multiple, systems are highly fragile and conflict disrupts health care. Médecins Sans Frontières (MSF) has been working to integrate HIV care in basic health services in such settings since 2000. We review the implementation of differentiated HIV care and treatment approaches in MSF-supported programmes in South Sudan (RoSS), Central African Republic (CAR) and Democratic Republic of Congo (DRC)...
July 21, 2017: Journal of the International AIDS Society
https://www.readbyqxmd.com/read/28768494/socio-cognitive-correlates-of-intention-to-use-toombak-a-cross-sectional-study-among-students-13-16%C3%A2-years-in-khartoum-state-sudan
#18
Hatim Mohammed Almahdi, Rouf Wahab Ali, Elwalid Fadul Nasir, Anne Nordrehaug Åstrøm
BACKGROUND: Toombak is a form of smokeless tobacco, locally made and consumed in Sudan. It is associated with a number of health hazards, particularly oral cancer. This study was set out to assess the prevalence and socio-demographic distribution of its use, and to explore the socio-cognitive factors affecting the intention to use Toombak among secondary school students in Khartoum State, Sudan. METHODS: A cross-sectional school-based study was conducted in 2013-2014 where schools were randomly selected using a one-stage stratified sampling procedure...
August 2, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28766375/powerlessness-normalization-and-resistance-a-foucauldian-discourse-analysis-of-women-s-narratives-on-obstetric-fistula-in-eastern-sudan
#19
Sarah Hamed, Beth-Maina Ahlberg, Jill Trenholm
Eastern Sudan has high prevalence of female circumcision and child marriage constituting a risk for developing obstetric fistula. Few studies have examined gender roles' relation with obstetric fistula in Sudan. To explore the associated power-relations that may put women at increased risk for developing obstetric fistula, we conducted nine interviews with women living with obstetric fistula in Kassala in eastern Sudan. Using a Foucauldian discourse analysis, we identified three discourses: powerlessness, normalization, and covert resistance...
August 1, 2017: Qualitative Health Research
https://www.readbyqxmd.com/read/28764736/quadriacanthus-species-monogenea-dactylogyridae-from-catfishes-teleostei-siluriformes-in-eastern-africa-new-species-new-records-and-first-insights-into-interspecific-genetic-relationships
#20
Kateřina Francová, Mária Seifertová, Radim Blažek, Milan Gelnar, Zuheir N Mahmoud, Eva Řehulková
BACKGROUND: African catfishes of the families Bagridae and Clariidae are known to be parasitized with monogeneans of Quadriacanthus Paperna, 1961 (Dactylogyridae). The genus remains taxonomically challenging due to its speciose nature and relatively wide host range representing two fish orders, i.e. Siluriformes and Osteoglossiformes, in Africa and Asia. Here, we investigated diversity of Quadriacanthus spp. parasitizing Clarias gariepinus (Burchell), Heterobranchus bidorsalis Geoffroy Saint-Hilaire, and Bagrus docmak (Forsskål) collected in the Lake Turkana (Kenya) and Nile River Basin (Sudan)...
August 1, 2017: Parasites & Vectors
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