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https://www.readbyqxmd.com/read/29458079/autofluorescence-a-potential-pitfall-in-immunofluorescence-based-inflammation-grading
#1
Jonas Wizenty, Muhammad Imtiaz Ashraf, Nadine Rohwer, Martin Stockmann, Sascha Weiss, Matthias Biebl, Johann Pratschke, Felix Aigner, Tilo Wuensch
Immunofluorescence (IF) staining of paraffin-embedded tissues is a frequently used method to answer research questions or even detect the abundance of a certain protein for diagnostic use. However, the signal originating from specific antibody-staining might be distorted by autofluorescence (AF) of the assessed tissue. Although the AF phenomenon is well known, its presence is often neglected by insufficient staining controls. In this study, we describe the existence of cellular AF in paraffin-embedded healthy and inflamed human and murine colonic tissues and present ways to reduce AF...
February 16, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29456823/health-hazards-related-to-soba-sewage-treatment-plant-sudan
#2
Rasha Osman Abdelwahab Abdelmoneim, Ranien Mohamed El Mortada Mahi Eldin Mahdi, Rawia Moawia Elamin Elahmer, Rogaya Adel Derar Elansary, Safaa Elsafi Widaa Mohamed, Said Hashim Ahmed Abdelgader, Sakina Salah Abbasher Ahmed, Sally Mohamed Alfatih Abdalrhman
The aim of this study was to determine the health hazards acquired by the residents nearby Soba sewage treatment plant. A descriptive cross-sectional study was carried out in Soba locality, Khartoum, Sudan. An interviewer-administrated questionnaire was assigned to 462 residents of the area living in four geographically distributed squares around the sewage plant. The data was analyzed in SPSS; Cronbach's alpha reliability scale of measurement was used to check the internal validity of six variables related to the quality of life...
December 31, 2017: Journal of Public Health in Africa
https://www.readbyqxmd.com/read/29455427/variation-in-cardiac-markers-and-electrocardiographic-alterations-in-young-calves-naturally-infected-with-bovine-tropical-theileriosis
#3
Kapil Kumar Gupta, Mukesh Srivastava, Vikrant Sudan, Shanker Kumar Singh, Soumen Choudhury, Daya Shanker
The present study was designed to assess the deleterious effects of bovine tropical theileriosis on the cardiovascular system and the consequent myocardial involvement in young calves. Myocardial effects in parasitic diseases are often neglected. Hemolytic anemia, associated secondary hypoxia, and vasculitis are cardinal features of bovine theileriosis. In the present study, electrocardiogram (ECG) alongside serum cardiac troponin I (cTnI) and creatinine phosphokinase-myocardial band (CPK-MB) concentrations were analyzed in infected, treated, and control groups of young calves...
February 17, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29454365/qualitative-evaluation-of-the-saleema-campaign-to-eliminate-female-genital-mutilation-and-cutting-in-sudan
#4
Andrea C Johnson, W Douglas Evans, Nicole Barrett, Howida Badri, Tamador Abdalla, Cody Donahue
BACKGROUND: Female genital mutilation and cutting (FGM/C, herein FGM) is a widespread and harmful practice. The Government developed a national campaign in Sudan, called Saleema, to change social norms discouraging FGM. Saleema translates to being "whole", healthy in body and mind, unharmed, intact, pristine, and untouched, in a God-given condition. An interim evaluation was conducted using focus groups among Sudanese adults. The primary aim was to explore perceptions of the Saleema poster exemplars and to assess if the desired themes were being communicated...
February 17, 2018: Reproductive Health
https://www.readbyqxmd.com/read/29453666/comments-on-a-mathematical-study-to-control-visceral-leishmaniasis-an-application-to-south-sudan
#5
E Iboi, K Okuneye, O Sharomi, A B Gumel
Deterministic (ordinary differential equation) models for the transmission dynamics of vector-borne diseases that incorporate disease-induced death in the host(s) population(s) are generally known to exhibit the phenomenon of backward bifurcation (where a stable disease-free equilibrium of the model coexists with a stable endemic equilibrium when the associated reproduction number of the model is less than unity). Further, it is well known that, in these models, the phenomenon of backward bifurcation does not occur when the disease-induced death rate is negligible (e...
February 16, 2018: Bulletin of Mathematical Biology
https://www.readbyqxmd.com/read/29453613/in-vitro-antioxidant-and-cytotoxic-activities-of-18-plants-from-the-erkowit-region-eastern-sudan
#6
Manar Adam, Gihan O M Elhassan, Sakina Yagi, Fatma Sezer Senol, Ilkay Erdogan Orhan, Abdel Azim Ahmed, Thomas Efferth
We investigated the antioxidant potential and cytotoxicity towards human CCRF-CEM leukemia cells of 57 extracts obtained from 18 plants collected in the Erkowit region, eastern Sudan. The antioxidant activity was determined by measuring the radical scavenging effects against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and N,N-dimethyl-p-phenylendiamine (DMPD), metal-chelation capacity, ferric-reducing (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) methods using ELISA microtiter assays. Total phenol and flavonoid amounts of the extracts were determined spectrophotometrically...
February 16, 2018: Natural Products and Bioprospecting
https://www.readbyqxmd.com/read/29446145/neuronal-ceroid-lipofuscinosis-in-salukis-is-caused-by-a-single-base-pair-insertion-in-cln8
#7
F Lingaas, O-A Guttersrud, E Arnet, A Espenes
Neuronal ceroid lipofuscinoses (NCLs) are heterogenic inherited lysosomal storage diseases that have been described in a number of species including humans, sheep, cattle, cats and a number of different dog breeds, including Salukis. Here we present a novel genetic variant associated with the disease in this particular breed of dog. In a clinical case, a Saluki developed progressive neurological signs, including disorientation, anxiety, difficulties in eating, seizures and loss of vision, and for welfare reasons, was euthanized at 22 months of age...
February 2018: Animal Genetics
https://www.readbyqxmd.com/read/29444779/estimating-the-met-need-for-emergency-obstetric-care-emoc-services-in-three-payams-of-torit-county-south-sudan-a-facility-based-retrospective-cross-sectional-study
#8
Pontius Bayo, Imose Itua, Suzie Paul Francis, Kofi Boateng, Elijo Omoro Tahir, Abdulmumini Usman
OBJECTIVE: To determine the met need for emergency obstetric care (EmOC) services in three Payams of Torit County, South Sudan in 2015 and to determine the frequency of each major obstetric complication. DESIGN: This was a retrospective cross-sectional study. SETTING: Four primary healthcare centres (PHCCs) and one state hospital in three payams (administrative areas that form a county) in Torit County, South Sudan. PARTICIPANTS: All admissions in the obstetrics and gynaecology wards (a total of 2466 patient admission files) in 2015 in all the facilities designated to conduct deliveries in the study area were reviewed to identify obstetric complications...
February 14, 2018: BMJ Open
https://www.readbyqxmd.com/read/29444310/radiological-hazard-indices-of-granitic-rocks-used-for-the-construction-of-buildings-from-nuba-mountains-sudan
#9
Nooreldin Fadol, Hajo Idriss, Isam Salih, Nserdin A Ragab, Safa Osman, Adam K Sam
This study was conducted to assess the level of radioactivity and the radiation hazards associated with granite rocks used for construction of buildings. The measurement of radioactivity content of the rock samples was performed with gamma-spectrometry equipped with Nal (TI) detector. From the results obtained in this study the average activity concentrations of 226Ra, 232Th and 40K were 20.64, 30.50 and 295.19 Bq kg-1, respectively. The absorbed dose rate in air at 1 m above ground level, the annual effective dose and the gamma index were determined with the aim to assess the possible radiological impact on inhabitants of dwellings built using such rocks...
February 12, 2018: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/29444079/sensitive-and-less-invasive-confirmatory-diagnosis-of-visceral-leishmaniasis-in-sudan-using-loop-mediated-isothermal-amplification-lamp
#10
Maowia Mukhtar, Sababil S Ali, Salah A Boshara, Audrey Albertini, Séverine Monnerat, Paul Bessell, Yasuyoshi Mori, Yutaka Kubota, Joseph M Ndung'u, Israel Cruz
BACKGROUND: Confirmatory diagnosis of visceral leishmaniasis (VL), as well as diagnosis of relapses and test of cure, usually requires examination by microscopy of samples collected by invasive means, such as splenic, bone marrow or lymph node aspirates. This causes discomfort to patients, with risks of bleeding and iatrogenic infections, and requires technical expertise. Molecular tests have great potential for diagnosis of VL using peripheral blood, but require well-equipped facilities and trained personnel...
February 14, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29438940/functional-paper-based-sers-substrate-for-rapid-and-sensitive-detection-of-sudan-dyes-in-herbal-medicine
#11
Mianmian Wu, Pan Li, Qingxia Zhu, Meiran Wu, Hao Li, Feng Lu
There has been an increasing demand for rapid and sensitive techniques for the identification of Sudan compounds that emerged as the most often illegally added fat-soluble dyes in herbal medicine. In this report, we have designed and fabricated a functionalized filter paper consisting of gold nanorods (GNRs) and mono-6-thio-cyclodextrin (HS-β-CD) as a surface-enhanced Raman spectroscopy (SERS) substrate, in which the GNR provides sufficient SERS enhancement, and the HS-β-CD with strong chemical affinity toward GNR provides the inclusion compound to capture hydrophobic molecules...
February 6, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29436822/detection-of-sudan-dyes-based-on-inner-filter-effect-with-reusable-conjugated-polymer-fibrous-membranes
#12
Ming Wu, Lijuan Sun, Kesong Miao, Yingzhong Wu, Li-Juan Fan
Developing effective methods for detecting illegal additives in food or seasoning is of great significance. In this study, a sensing strategy for selective detection of Sudan dyes was designed based on fluorescence inner filter effect (IFE) by using poly(phenylenevinylene) (PPV) solid materials and combing with optimized experimental protocol. Two types of fluorescent solid materials, electrospun fibrous membranes and drop-cast films, were fabricated with PPV as the fluorophore and poly(vinyl alcohol) (PVA) as the matrix...
February 13, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29435803/rifampicin-prevents-sh-sy5y-cells-from-rotenone-induced-apoptosis-via-the-pi3k-akt-gsk-3%C3%AE-creb-signaling-pathway
#13
Xia Wu, Yanran Liang, Xiuna Jing, Danyu Lin, Ying Chen, Tianen Zhou, Sudan Peng, Dezhi Zheng, Zhifen Zeng, Ming Lei, Kaixun Huang, Enxiang Tao
In addition to its original application for treating tuberculosis, rifampicin has multiple potential neuroprotective effects in chronic neurodegenerative diseases including Parkinson's disease (PD) and Alzheimer's disease. Inflammatory reactions and the PI3K/Akt pathway are strongly implicated in dopaminergic neuronal death in PD. This study aims to investigate whether rifampicin protects rotenone-lesioned SH-SY5Y cells via regulating PI3K/Akt/GSK-3β/CREB pathway. Rotenone-treated SH-SY5Y cells were used as the cell model to investigate the neuroprotective effects of rifampicin...
February 12, 2018: Neurochemical Research
https://www.readbyqxmd.com/read/29434731/correlation-between-ppar-%C3%AE-methylation-level-in-peripheral-blood-and-inflammatory-factors-of-nafld-patients-with-dm
#14
Jianghua Ju, Qingxian Huang, Jing Sun, Yongcheng Jin, Wenjie Ma, Xiaohui Song, Huibo Sun, Wenhui Wang
The correlation between the methylation levels of peroxisome proliferator-activated receptor-α (PPAR-α) in the peripheral blood and the inflammatory factors associated with non-alcoholic fatty liver disease (NAFLD) patients with diabetes mellitus (DM) was investigated. Thirty-two samples of normal liver tissues (group N) and 35 samples of liver tissues from NAFLD patients with DM (group M) were used for the present study. The levels of alanine transaminase (ALT) and aspartate transaminase (AST) were measured using commercially available kits...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29427658/puerarin-protects-against-heart-failure-induced-by-pressure-overload-through-mitigation-of-ferroptosis
#15
Bei Liu, Chunxia Zhao, Hongkun Li, Xiaoqian Chen, Yu Ding, Sudan Xu
Heart failure (HF) is the end stage of cardiovascular disease and is characterized by the loss of myocytes caused by cell death. Puerarin has been found to improve HF clinically, and animal study findings have confirmed its anti-cell-death properties. However, the underlying mechanisms remain unclear, especially with respect to the impact on ferroptosis, a newly defined mechanism of iron-dependent non-apoptotic cell death in HF. Here, ferroptosis-like cell death was observed in erastin- or isoprenaline (ISO)-treated H9c2 myocytes in vitro and in rats with aortic banding inducing HF, characterized by reduced cell viability with increased lipid peroxidation and labile iron pool...
February 7, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29426590/detection-and-molecular-characterization-of-tick-borne-pathogens-infecting-sheep-and-goats-in-blue-nile-and-west-kordofan-states-in-sudan
#16
Seung-Hun Lee, Ehab Mossaad, Abdalla Mohamed Ibrahim, Ahmed Ali Ismail, Paul Franck Adjou Moumouni, Mingming Liu, Aaron Edmond Ringo, Yang Gao, Huanping Guo, Jixu Li, Artemis Efstratiou, Peter Musinguzi, Tamador E E Angara, Keisuke Suganuma, Noboru Inoue, Xuenan Xuan
Tick-borne pathogens (TBPs) are common in livestock of sub-Saharan Africa. However, information regarding TBPs in sheep and goats in Sudan is limited. In this study, 178 blood samples of sheep and goats in Blue Nile and West Kordofan states were investigated for TBPs using PCR. Overall, 110 (61.8%) samples were found to be infected with at least one of the following pathogens: Anaplasma ovis, Theileria ovis, and Ehrlichia ruminantium. Babesia ovis and T. lestoquardi were not identified. A. ovis was the most prevalent pathogen (n = 107, 60...
February 1, 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29426503/aqueous-biphasic-systems-formed-by-deep-eutectic-solvent-and-new-type-salts-for-the-high-performance-extraction-of-pigments
#17
Hongmei Zhang, Yuzhi Wang, Yigang Zhou, Jing Chen, Xiaoxiao Wei, Panli Xu
Deep eutectic solvent (DES) composed of polypropylene glycol 400 (PPG 400) and tetrabutylammonium bromide (TBAB) was combined with a series of new-type salts such as quaternary ammonium salts, amino acid and polyols to form Aqueous Biphasic Systems (ABSs). Phase-forming ability of the salts was investigated firstly. The results showed that polyols had a relatively weak power to produce phases within studied scopes. And the shorter of carbon chain length of salts, the easier to obtain phase-splitting. Then partitioning of three pigments in PPG 400/betaine-based ABSs was addressed to investigate the effect of pigments' hydrophobicity on extraction efficiency...
May 1, 2018: Talanta
https://www.readbyqxmd.com/read/29422946/evaluating-the-communities-care-program-best-practice-for-rigorous-research-to-evaluate-gender-based-violence-prevention-and-response-programs-in-humanitarian-settings
#18
N Glass, N Perrin, A Clough, A Desgroppes, F N Kaburu, J Melton, A Rink, S Read-Hamilton, M Marsh
Background: Gender-based violence (GBV) is a significant issue for women and girls in humanitarian settings. Innovative primary prevention programs are being developed and implemented with existing response programs to change harmful social norms that sustain GBV in humanitarian settings. Social norms are expectations of how women, men, girls and boys should behave, who should have power and control over behavior, and how families and communities value women and girls and support their rights and opportunities...
2018: Conflict and Health
https://www.readbyqxmd.com/read/29415657/assessment-of-blood-pressure-control-in-adult-hypertensive-patients-in-eastern-sudan
#19
Saeed M Omar, Osama Elnour, Gamal K Adam, Osman E Osman, Ishag Adam
BACKGROUND: The rate of blood pressure (BP) control in adult hypertensive patients is poor and the reasons for poor control of BP pressure are not fully understood globally. This study aimed to assess the rate and factors associated with BP control in adult hypertensive patients in Sudan. METHODS: A hospital-based cross-sectional study was conducted in adult hypertensive Sudanese patients at Gadarif Hospital in eastern Sudan from November 2016 to March 2017. Information on sociodemographic characteristics of the participants, comorbidities, antihypertensive medication, and adherence to antihypertensive medication was gathered from patients using a questionnaire...
February 7, 2018: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/29412917/comparative-analysis-of-hibiscus-sabdariffa-roselle-hot-and-cold-extracts-in-respect-to-their-potential-for-%C3%AE-glucosidase-inhibition
#20
Dalia M Rasheed, Andrea Porzel, Andrei Frolov, Hesham R El Seedi, Ludger A Wessjohann, Mohamed A Farag
Roselle (Hibiscus sabdariffa) is a functional food with potential health benefits, consumed either as hot or cold beverage. To ensure quality control of its various products, accurate measurement of active metabolites is warranted. Herein, we propose a combination of ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) and nuclear magnetic resonance (NMR) analytical platforms for the untargeted characterization of metabolites in two roselle cultivars, Aswan and Sudan-1. The analyses revealed 33 metabolites, including sugars, flavonoids, anthocyanins, phenolic and aliphatic organic acids...
June 1, 2018: Food Chemistry
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