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https://www.readbyqxmd.com/read/28738471/-association-between-phage-mediated-shiga-toxin-and-molecular-distribution-of-crispr-in-escherichia-coli-o26-%C3%A2-h11-or-nm
#1
J Z Long, Y K Xu, G C Duan, W J Liang, H Y Liu, S Y Chen, Y L Xi, P F Wang, Y F Wang
Objective: To investigate the association between phage-mediated shiga toxin and molecular distribution of CRISPR in Escherichia (E.) coli O26∶H11 or NM. Methods: A total of 135 E. coli O26 ∶ H11 or NM strains were collected from NCBI database. Software CRT and CRISPR Finder were used to extract CRISPR and Excel was used to assign the spacer of unique number and type CRISPR. And the relationship between CRISPR and stx phage was analyzed. Results: All the 135 E. coli O26 ∶ H11 or NM strains had the CRISPR...
July 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28727722/an-attenuated-shigella-mutant-lacking-the-rna-binding-protein-hfq-provides-cross-protection-against-shigella-strains-of-broad-serotype
#2
Jiro Mitobe, Ritam Sinha, Soma Mitra, Dhrubajyoti Nag, Noriko Saito, Ken Shimuta, Nobuo Koizumi, Hemanta Koley
Few live attenuated vaccines protect against multiple serotypes of bacterial pathogen because host serotype-specific immune responses are limited to the serotype present in the vaccine strain. Here, immunization with a mutant of Shigella flexneri 2a protected guinea pigs against subsequent infection by S. dysenteriae type 1 and S. sonnei strains. This deletion mutant lacked the RNA-binding protein Hfq leading to increased expression of the type III secretion system via loss of regulation, resulting in attenuation of cell viability through repression of stress response sigma factors...
July 20, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28726627/mucus-activatable-shiga-toxin-genotype-stx2d-in-escherichia-coli-o157-h7
#3
Sergio Sánchez, María Teresa Llorente, Laura Herrera-León, Raquel Ramiro, Sandra Nebreda, María Antonia Remacha, Silvia Herrera-León
We identified the mucus-activatable Shiga toxin genotype stx2d in the most common hemolytic uremic syndrome-associated Escherichia coli serotype, O157:H7. stx2d was detected in a strain isolated from a 2-year-old boy with bloody diarrhea in Spain, and whole-genome sequencing was used to confirm and fully characterize the strain.
August 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28726488/use-of-an-electrostatic-spraying-system-or-the-sprayed-lethality-in-container-method-to-deliver-antimicrobial-agents-onto-the-surface-of-beef-subprimals-to-control-shiga-toxin-producing-escherichia-coli
#4
J Max Stella, John B Luchansky, Kelsey Miller, Bradley A Shoyer, Laura E Shane, Lianna McGeary, Manuela Osoria, Laura J Stahler, Nicholas J Sevart, Randall K Phebus, Harshavardhan Thippareddi, Anna C S Porto-Fett
The efficacy of an electrostatic spraying system (ESS) and/or the sprayed lethality in container (SLIC) method to deliver antimicrobial agents onto the surface of beef subprimals to reduce levels of Shiga toxin-producing Escherichia coli (STEC) was evaluated. Beef subprimals were surface inoculated (lean side; ca. 5.8 log CFU per subprimal) with 2 mL of an eight-strain cocktail comprising single strains of rifampin-resistant (100 μg/mL) STEC (O26:H11, O45:H2, O103:H2, O104:H4, O111:H(-), O121:H19, O145:NM, and O157:H7)...
July 20, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28725563/atypical-hemolytic-uremic-syndrome-triggered-by-varicella-infection
#5
Pauline Condom, Jean-Michel Mansuy, Stéphane Decramer, Jacques Izopet, Catherine Mengelle
Varicella Zoster Virus (VZV) is a well-known virus that belongs to the Herpesviridae family which induces a self-limited disease except in specific cases in particular among stem cell transplant patients. This virus is not known however to trigger atypical Hemolytic Uremic Syndrome (aHUS). Here we report the case of a six-year-old boy who was hospitalized with fever and abdominal pains associated to pruritic and vesicular rash, thrombocytopenia and acute renal failure. He was diagnosed with aHUS precipitated by varicella virus...
2017: IDCases
https://www.readbyqxmd.com/read/28718802/ouabain-protects-human-renal-cells-against-the-cytotoxic-effects-of-shiga-toxin-type-2-and-subtilase-cytotoxin
#6
María M Amaral, Magalí C Girard, Romina S Álvarez, Adrienne W Paton, James C Paton, Horacio A Repetto, Flavia Sacerdoti, Cristina A Ibarra
Hemolytic uremic syndrome (HUS) is one of the most common causes of acute renal failure in children. The majority of cases are associated with Shiga toxin (Stx)-producing Escherichia coli (STEC). In Argentina, HUS is endemic and presents the highest incidence rate in the world. STEC strains expressing Stx type 2 (Stx2) are responsible for the most severe cases of this pathology. Subtilase cytotoxin (SubAB) is another STEC virulence factor that may contribute to HUS pathogenesis. To date, neither a licensed vaccine nor effective therapy for HUS is available for humans...
July 18, 2017: Toxins
https://www.readbyqxmd.com/read/28713792/shiga-toxin-producing-escherichia-coii-in-slaughtered-pigs-and-pork-products
#7
Lia Bardasi, Roberta Taddei, Ilaria Fiocchi, Maria Francesca Pelliconi, Mattia Ramini, Elena Toschi, Giuseppe Merialdi
During the years 2015-2016, 83 faecal samples were collected at slaughter from pigs reared in farms located in Central-Northern Italy. During the years 2014-2016 a total of 562 pork products [465 not-readyto-eat (NRTE) and 97 ready-to-eat (RTE) products] were collected from retail outlets, large retailers and processing plants. The samples were analysed according to ISO TS 13136:2012. Out of 83 swine faecal samples, 77 (92.8%) resulted stx-positive by real time polymerase chain reaction (PCR), 5 stx2+ and 1 stx1+ Shiga toxin-producing Escherichia coli (STEC) strains were isolated...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28693311/piglet-colibacillosis-diagnosis-by-multiplex-pcr-and-immunohistochemistry-of-paraffin-embedded-tissues
#8
Cíntia De Lorenzo, Caroline P de Andrade, Verônica S L Machado, Matheus V Bianchi, Veronica M Rolim, Raquel A S Cruz, David Driemeier
Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in pigs, a condition termed colibacillosis, in which these bacteria should adhere and produce enterotoxins. The aim of this study was to optimize multiplex polymerase chain reaction (PCR) and immunohistochemistry (IHC) analyses of paraffin-embedded material to detect pathogenic strains of E. coli causing colibacillosis in pigs, which requires the expression of at least one fimbria and one toxin of the agent. Multiplex PCR was optimized for fimbriae (F18, F4, F6, F5, and F41) and toxins [types A and B heat-stable toxins (STaP and STb), heat-labile toxin (LT), and type 2 Shiga toxin (STx2e)], and IHC was optimized for an anti-E...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28687651/characterization-of-shiga-toxin-producing-escherichia-coli-strains-of-o91-serogroup-isolated-from-food-and-environmental-samples
#9
Peter C H Feng, Sabine Delannoy, David W Lacher, Joseph M Bosilevac, Patrick Fach, Lothar Beutin
Shiga toxin-producing Escherichia coli (STEC) of the O91:H21 serotype has caused severe infections including hemolytic uremic syndrome. Strains of the O91 serogroup have been isolated from food, animals and the environment worldwide, but are not well characterized. We used a microarray and other molecular assays to examine 49 O91 strains (environmental, food and clinical) for virulence potential and phylogenetic relationships. Most of the isolates were identified to be strains of O91:H21 and O91:H14 serotype, with a few O91:H10 strains and one O91:H9 strain...
July 7, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28683136/generality-of-toxins-in-defensive-symbiosis-ribosome-inactivating-proteins-and-defense-against-parasitic-wasps-in-drosophila
#10
Matthew J Ballinger, Steve J Perlman
While it has become increasingly clear that multicellular organisms often harbor microbial symbionts that protect their hosts against natural enemies, the mechanistic underpinnings underlying most defensive symbioses are largely unknown. Spiroplasma bacteria are widespread associates of terrestrial arthropods, and include strains that protect diverse Drosophila flies against parasitic wasps and nematodes. Recent work implicated a ribosome-inactivating protein (RIP) encoded by Spiroplasma, and related to Shiga-like toxins in enterohemorrhagic Escherichia coli, in defense against a virulent parasitic nematode in the woodland fly, Drosophila neotestacea...
July 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28683061/notes-from-the-field-an-outbreak-of-shiga-toxin-producing-escherichia-coli-o121-infections-associated-with-flour-canada-2016-2017
#11
Vanessa Morton, Joyce M Cheng, Davendra Sharma, Ashley Kearney
No abstract text is available yet for this article.
July 7, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28682564/-complement-factor-b-mutation-in-atypical-hemolytic-uremic-syndrome-rare-cause-of-rare-disease
#12
Luca Visconti, Valeria Cernaro, Gianluigi Ardissino, Martina Sgarbanti, Domenico Ferrara, Giuseppe Visconti, Domenico Santoro, Michele Buemi
Hemolytic uremic syndrome (HUS) is a rare disease characterized by microangiopathic hemolysis, platelet consumption and multiple organ failure with predominant renal involvement. In the most of cases (85-90%), it is associated with enteric infection due to Shiga-toxin or verocytotoxin (STEC-VTEC)-producer Escherichia coli. Rarely, in about 10-15% of cases, HUS develops in the presence of a disorder of alternative complement pathway regulation and it is defined atypical (aHUS). We describe the case of a 65-year-old man who came to our attention with a clinical presentation of aHUS and a clinical course characterized by rapidly progressive acute renal failure (ARF), which required renal replacement treatments, and by a stable clinical picture of hematological impairment as a marker of a non-severe and self-limiting form...
April 2017: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
https://www.readbyqxmd.com/read/28681491/magnetic-resonance-imaging-appearance-of-the-brain-and-cervical-spinal-cord-in-an-edema-disease-affected-pig
#13
Lucía Dieste-Pérez, Tetyda P Dobak, Federico R Vilaplana Grosso, Wilhelmina Bergmann, Tijs J Tobias
A 7-week-old male pig was presented with signs of a central nervous system disorder. An MRI of the head and cervical spine was performed immediately after euthanasia. The MRI revealed multifocal bilaterally symmetric T2-weighted hyperintense lesions in the brain and spinal cord, likely due to a toxic metabolic process. Histopathological examination supported the MRI findings and confirmed the diagnosis of edema disease due to Shiga-like toxin produced by Escherichia coli. This is the first case published of the MRI findings in an edema disease affected pig...
July 6, 2017: Veterinary Radiology & Ultrasound
https://www.readbyqxmd.com/read/28676794/bacteriophages-contribute-to-the-spread-of-antibiotic-resistance-genes-among-foodborne-pathogens-of-the-enterobacteriaceae-family-a-review
#14
REVIEW
Anna Colavecchio, Brigitte Cadieux, Amanda Lo, Lawrence D Goodridge
Foodborne illnesses continue to have an economic impact on global health care systems. There is a growing concern regarding the increasing frequency of antibiotic resistance in foodborne bacterial pathogens and how such resistance may affect treatment outcomes. In an effort to better understand how to reduce the spread of resistance, many research studies have been conducted regarding the methods by which antibiotic resistance genes are mobilized and spread between bacteria. Transduction by bacteriophages (phages) is one of many horizontal gene transfer mechanisms, and recent findings have shown phage-mediated transduction to be a significant contributor to dissemination of antibiotic resistance genes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28675605/intrahost-milieu-modulates-production-of-outer-membrane-vesicles-vesicle-associated-shiga-toxin-2a-and-cytotoxicity-in-escherichia-coli-o157-h7-and-o104-h4
#15
Andreas Bauwens, Lisa Kunsmann, Monika Marejková, Wenlan Zhang, Helge Karch, Martina Bielaszewska, Alexander Mellmann
Outer membrane vesicles (OMVs) are important virulence tools of enterohaemorrhagic Escherichia coli (EHEC), but other biological functions of these nanostructures are unknown. We tested the hypothesis that modulation of OMV production enables EHEC to resist the intrahost environment during infection by investigating if simulated human gastrointestinal conditions affect OMV production in EHEC O157:H7 and O104:H4. All the conditions tested including a low pH, simulated ileal and colonic media, presence of mucin, intestinal epithelial cell lysate or antimicrobial peptides, as well as iron limitation, significantly increased OMV production by these pathogens...
July 4, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28656639/comparison-of-immunomagnetic-separation-beads-for-detection-of-six-non-o157-shiga-toxin-producing-escherichia-coli-serogroups-in-different-matrices
#16
Autumn L Kraft, David W Lacher, Weilin L Shelver, Julie S Sherwood, Teresa M Bergholz
Immunomagnetic separation used with culture based methods has been a useful technique in the detection of pathogens. However, previous studies have not answered many of the necessary questions for real world applications. The objective of this study was to assess the efficacy of different IMS bead types in recovery of the correct serogroup from a mixture of big six non-O157 STEC strains. To determine the impact of different matrices on recovery, samples of sterile phosphate buffered saline (PBS), sterile and non-sterile cattle feces, ground beef, and lettuce were inoculated with 10 CFU ml(-1) mixture of isolates representing the six serogroups...
June 28, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28643674/microbiological-and-serological-control-of-escherichia-coli-o157-h7-in-kindergarten-staff-in-buenos-aires-city-and-suburban-areas
#17
Romina J Fernández-Brando, María Marta Amaral, Andrés E Ciocchini, Leticia V Bentancor, Jorge A Trelles, Marcelo Da Rocha, Martín Landriel, Mariana Ugarte, Gabriel Briones, Cristina Ibarra, Marina S Palermo
Shiga toxin (Stx)-producing Escherichia coli (STEC) infections are implicated in the development of the life-threatening hemolytic-uremic syndrome (HUS). Despite the magnitude of the social and economic problems caused by HUS, no licensed vaccine or effective therapy is currently available for human use. Prevention of STEC infections continues being the most important measure to reduce HUS incidence. This is especially true for Argentina where HUS incidence among children is extremely high and shows an endemic pattern...
2017: Medicina
https://www.readbyqxmd.com/read/28630857/effectiveness-of-a-commercial-lactic-acid-bacteria-intervention-applied-to-inhibit-shiga-toxin-producing-escherichia-coli-on-refrigerated-vacuum-aged-beef
#18
Katie R Kirsch, Tamra N Tolen, Jessica C Hudson, Alejandro Castillo, Davey Griffin, T Matthew Taylor
Because of their antagonistic activity towards pathogenic and spoilage bacteria, some members of the lactic acid bacteria (LAB) have been evaluated for use as food biopreservatives. The objectives of this study were to assess the antimicrobial utility of a commercial LAB intervention against O157 and non-O157 Shiga-toxigenic E. coli (STEC) on intact beef strip loins during refrigerated vacuum aging and determine intervention efficacy as a function of mode of intervention application. Prerigor strip loins were inoculated with a cocktail (8...
2017: International journal of food science
https://www.readbyqxmd.com/read/28628436/contaminated-stream-water-as-source-for-escherichia-coli-o157-illness-in-children
#19
William S Probert, Glen M Miller, Katya E Ledin
In May 2016, an outbreak of Shiga toxin-producing Escherichia coli O157 infections occurred among children who had played in a stream flowing through a park. Analysis of E. coli isolates from the patients, stream water, and deer and coyote scat showed that feces from deer were the most likely source of contamination.
July 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28625983/strain-level-metagenomic-analysis-of-the-fermented-dairy-beverage-nunu-highlights-potential-food-safety-risks
#20
Aaron M Walsh, Fiona Crispie, Kareem Daari, Orla O'Sullivan, Jennifer C Martin, Cornelius T Arthur, Marcus J Claesson, Karen P Scott, Paul D Cotter
The rapid detection of pathogenic strains in food products is essential for the prevention of disease outbreaks. It has already been demonstrated that whole metagenome shotgun sequencing can be used to detect pathogens in food but, until recently, strain-level detection of pathogens has relied on whole metagenome assembly, which is a computationally demanding process. Here, we demonstrate that three short read alignment-based methods, MetaMLST, PanPhlAn, and StrainPhlAn, can accurately, and rapidly, identify pathogenic strains in spinach metagenomes which were intentionally spiked with Shiga toxin-producing Escherichia coli in a previous study...
June 16, 2017: Applied and Environmental Microbiology
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