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https://www.readbyqxmd.com/read/27908998/whole-genome-sequencing-applied-to-the-molecular-epidemiology-of-shiga-toxin-producing-escherichia-coli-o157-h7-in-argentina
#1
Claudia Carolina Carbonari, Nahuel Fittipaldi, Sarah Teatero, Taryn B T Athey, Luis Pianciola, Marcelo Masana, Roberto G Melano, Marta Rivas, Isabel Chinen
Shiga toxin-producing Escherichia coli strains are worldwide associated with sporadic human infections and outbreaks. In this work, we report the availability of high-quality draft whole-genome sequences for 19 O157:H7 strains isolated in Argentina.
December 1, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27904864/nephrotic-range-proteinuria-and-peripheral-edema-in-a-child-not-only-idiopathic-nephrotic-syndrome
#2
Valentina Dolcemascolo, Marina Vivarelli, Manuela Colucci, Francesca Diomedi-Camassei, Rossella Piras, Marta Alberti, Francesco Emma
Hemolytic uremic syndrome (HUS) is defined by the simultaneous occurrence of hemolytic anemia, thrombocytopenia, and acute kidney injury due to thrombotic microangiopathy (TMA) mainly occurring in renal and cerebral microvessels. Although the most common cause of HUS in children is Shiga toxin-producing Escherichia coli, atypical forms in which Shiga toxin is not the trigger may occur. Research over the last few years has shown that complement dysregulation secondary to mutations of genes coding for proteins involved in the regulation of the alternative pathway of complement account for most forms of atypical HUS (aHUS)...
September 2016: Case Reports in Nephrology and Dialysis
https://www.readbyqxmd.com/read/27893870/associations-between-hydration-status-intravenous-fluid-administration-and-outcomes-of-patients-infected-with-shiga-toxin-producing-escherichia-coli-a-systematic-review-and-meta-analysis
#3
Silviu Grisaru, Jianling Xie, Susan Samuel, Lisa Hartling, Phillip I Tarr, David Schnadower, Stephen B Freedman
Importance: The associations between hydration status, intravenous fluid administration, and outcomes of patients infected with Shiga toxin-producing Escherichia coli (STEC) remain unclear. Objective: To determine the relationship between hydration status, the development and severity of hemolytic uremic syndrome (HUS), and adverse outcomes in STEC-infected individuals. Data Sources: MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials via the OvidSP platform, PubMed via the National Library of Medicine, CINAHL Plus with full text, Scopus, Web of Science, ClinicalTrials...
November 28, 2016: JAMA Pediatrics
https://www.readbyqxmd.com/read/27891338/non-o157-h7-shiga-toxin-producing-diarrhoeagenic-escherichia-coli-stec-in-southern-india-a-tinderbox-for-starting-epidemic
#4
Shashank Purwar, Subrana Roy, Sharada Metgud
INTRODUCTION: Outbreaks due to non-O157:H7 Shiga toxin producing Escherichia coli (STEC) resulting in Haemolytic Uraemic Syndrome (HUS) have garnered much attention because of associated mortality transcending across continents and also because diarrhoea due to E.coli itself is rare in developed countries. The actual incidence of non-O157:H7 STEC in sporadic acute diarrhoea is not fully elucidated, both in developing as well as in developed countries. Due to larger extent of faecal-oral transmission in developing countries it is prudent to look for non-O157: H7 STEC in such epidemiological settings because of very high potential to spread across larger geographical regions and cause life threatening illness...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27891181/whole-genome-sequencing-and-comparative-genomic-analysis-of-escherichia-coli-o91-strains-isolated-from-symptomatic-and-asymptomatic-human-carriers
#5
Taesoo Kwon, Young-Seok Bak, Young-Hee Jung, Young-Bin Yu, Jong Tae Choi, Cheorl-Ho Kim, Jung-Beom Kim, Won Kim, Seung-Hak Cho
BACKGROUND: The Shiga toxin-producing Escherichia coli (STEC) O91:H21 strains NCCP15736 and NCCP15737 were isolated during a single outbreak in Korea, NCCP15736 from a symptomatic carrier and NCCP15737 from an asymptomatic carrier. To investigate genomic differences between the two strains, we performed whole-genome sequencing of both strains and conducted a comparative genomic analysis. RESULTS: Using the Illumina HiSeq 2000 platform and Rapid Annotation using the Subsystem Technology (RAST) server, whole-genome sequences of NCCP15736 and NCCP15737 were obtained and annotated...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27880834/overexpressed-proteins-in-hypervirulent-clade-8-and-clade-6-strains-of-escherichia-coli-o157-h7-compared-to-e-coli-o157-h7-edl933-clade-3-strain
#6
Natalia Amigo, Qi Zhang, Ariel Amadio, Qunjie Zhang, Wanderson M Silva, Baiyuan Cui, Zhongjian Chen, Mariano Larzabal, Jinlong Bei, Angel Cataldi
Escherichia coli O157:H7 is responsible for severe diarrhea and hemolytic uremic syndrome (HUS), and predominantly affects children under 5 years. The major virulence traits are Shiga toxins, necessary to develop HUS and the Type III Secretion System (T3SS) through which bacteria translocate effector proteins directly into the host cell. By SNPs typing, E. coli O157:H7 was separated into nine different clades. Clade 8 and clade 6 strains were more frequently associated with severe disease and HUS. In this study, we aimed to identify differentially expressed proteins in two strains of E...
2016: PloS One
https://www.readbyqxmd.com/read/27879643/structures-and-ribosomal-interaction-of-ribosome-inactivating-proteins
#7
REVIEW
Wei-Wei Shi, Amanda Nga-Sze Mak, Kam-Bo Wong, Pang-Chui Shaw
Ribosome-inactivating proteins (RIPs) including ricin, Shiga toxin, and trichosanthin, are RNA N-glycosidases that depurinate a specific adenine residue (A-4324 in rat 28S ribosomal RNA, rRNA) in the conserved α-sarcin/ricin loop (α-SRL) of rRNA. RIPs are grouped into three types according to the number of subunits and the organization of the precursor sequences. RIPs are two-domain proteins, with the active site located in the cleft between the N- and C-terminal domains. It has been found that the basic surface residues of the RIPs promote rapid and specific targeting to the ribosome and a number of RIPs have been shown to interact with the C-terminal regions of the P proteins of the ribosome...
November 21, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27869602/secondary-shiga-toxin-producing-escherichia-coli-infection-japan-2010-2012
#8
Tomoko Morita-Ishihara, Sunao Iyoda, Atsushi Iguchi, Makoto Ohnishi
To evaluate the potential public health risk caused by secondary Shiga toxin-producing Escherichia coli (STEC) infections in Japan, we investigated the prevalence and characteristics of STEC isolated from healthy adults during 2010-2012. Although prevalence among healthy adults was high, most STEC organisms displayed characteristics rarely found in isolates from symptomatic patients.
December 2016: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/27869414/prevalence-and-clinical-course-of-typical-hemolytic-uremic-syndrome-among-sibling
#9
Alfredo Eymann, Paula Coccia, Claudia Raddavero, Gabriela Lafi, Verónica Ferraris, José Ramírez, Jorge Ferraris
Introduction: Hemolytic uremic syndrome (HUS) isaninfectious disease caused by Shiga toxin-producing Escherichia coli. The objective of this study was to assess the risk of transmission and clinical course between siblings with typical HUS. Population and methods: Medical records of children with typical HUS between 1997 and 2012 were reviewed. Sibling pairs were established as inclusion criteria. A severity score was defined. Results: A total of 133 patients with HUS were recorded; 40 had siblings and 4 progressed to HUS (10%)...
December 1, 2016: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/27863950/evaluation-of-the-shiga-toxin-quik-chek-and-immunocard-stat-ehec-as-screening-tools-for-the-detection-of-shiga-toxin-in-fecal-specimens
#10
Megan Staples, Ning-Xia Fang, Rikki Ma Graham, Helen V Smith, Amy V Jennison
In this study we evaluated the performance of the SHIGA TOXIN QUIK CHEK (Techlab®, Blacksburg, VA) and the ImmunoCard STAT! Enterohaemorrhagic E. coli (EHEC) (Meridian BioScience, Cincinnati, OH, USA) assays as methods for qualitatively detecting the presence of Shiga toxin in human fecal specimens. A multiplex PCR for the detection of stx1 and stx2 was used as the standard for comparison. The SHIGA TOXIN QUIK CHEK detected all known Shiga toxin subtypes with the exception of Stx2f, while the ImmunoCard STAT! EHEC was unable to identify four of the seven Stx2 subtypes, including Stx2b and Stx2d...
March 10, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27857935/synchronous-disease-kinetics-in-a-murine-model-for-enterohemorrhagic-e-coli-infection-using-food-borne-inoculation
#11
Laurice J Flowers, Elsa N Bou Ghanem, John M Leong
Upon colonization of the intestinal epithelium, the attaching and effacing (AE) pathogen Enterohemorrhagic Escherichia coli (EHEC) effaces microvilli and forms pedestal-like structures beneath the adherent bacterium. The production of one of its virulence factors, the phage-encoded Shiga toxin (Stx) results in systemic disease, including the development of renal failure. Although EHEC does not productively infect conventional mice, EHEC infection can be modeled in mice utilizing a derivative of the natural murine AE pathogen Citrobacter rodentium (CR)...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27854514/subtyping-escherichia-coli-virulence-genes-isolated-from-feces-of-beef-cattle-and-clinical-cases-in-alberta
#12
Renata Tostes, Noriko Goji, Kingsley Amoako, Linda Chui, John Kastelic, Rebekah DeVinney, Kim Stanford, Tim Reuter
Clinical outcomes of Shiga toxin (stx)-producing Escherichia coli infection are largely determined by virulence gene subtypes. This study used a polymerase chain reaction (PCR)-pyrosequencing assay to analyze single-nucleotide polymorphisms for subtyping three major virulence genes (stx1, stx2, eae) of pathogenic E. coli (O157, O26, O111, and O103) isolated from cattle over a 2-year interval (n = 465) and human clinical cases (n = 42) in western Canada. Most bovine isolates were PCR positive for at least one target virulence gene (367/465), whereas 100% of human isolates harbored eae in combination with at least one stx gene...
November 17, 2016: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/27853704/molecular-and-phylogenetic-characterization-of-non-o157-shiga-toxin-producing-escherichia-coli-strains-in-china
#13
Xiangning Bai, Bin Hu, Yanmei Xu, Hui Sun, Ailan Zhao, Pengbin Ba, Shanshan Fu, Ruyue Fan, Yujuan Jin, Hong Wang, Qiusheng Guo, Xuebin Xu, Shan Lu, Yanwen Xiong
Shiga toxin-producing Escherichia coli (STEC) causes diarrhea and hemorrhagic colitis with life-threatening complications, such as hemolytic uremic syndrome. The aim of this study was to assess the molecular epidemiologic features of non-O157 STEC strains from different resources in China and illustrate the role of animal reservoirs or animal-derived foodstuffs in human STEC infections. A collection of 301 non-O157 STEC isolates from domestic and wild animals (i.e., cattle, goat, pig, yak, pika, and antelope), raw meats (i...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27842895/transmission-pathways-for-sporadic-shiga-toxin-producing-e-coli-infections-a-systematic-review-and-meta-analysis
#14
Erica Kintz, Julii Brainard, Lee Hooper, Paul Hunter
BACKGROUND: Shiga-toxin E. coli infections remain a public health concern because of the severity of the gastrointestinal illness and associated complications. Transmission pathways are typically elucidated from outbreaks, with foodborne transmission the primary source. However, most STEC cases are sporadic. This systematic review aimed to identify the most common pathways for sporadic STEC transmission and quantify their importance. METHODS: We systematically reviewed epidemiological studies of sporadic (non-outbreak) STEC cases that investigated potential risk factors...
January 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/27829218/cellular-effects-of-fluorodeoxyglucose-global-changes-in-the-lipidome-and-alteration-in-intracellular-transport
#15
Simona Kavaliauskiene, Maria Lyngaas Torgersen, Anne Berit Dyve Lingelem, Tove Irene Klokk, Tuulia Lintonen, Helena Simolin, Kim Ekroos, Tore Skotland, Kirsten Sandvig
2-fluoro-2-deoxy-D-glucose (FDG), labeled with 18F radioisotope, is the most common imaging agent used for positron emission tomography (PET) in oncology. However, little is known about the cellular effects of FDG. Another glucose analogue, 2-deoxy-D-glucose (2DG), has been shown to affect many cellular functions, including intracellular transport and lipid metabolism, and has been found to improve the efficacy of cancer chemotherapeutic agents in vivo. Thus, in the present study, we have investigated cellular effects of FDG with the focus on changes in cellular lipids and intracellular transport...
November 4, 2016: Oncotarget
https://www.readbyqxmd.com/read/27826079/purified-stx-and-%C3%AE-phage-initiator-o-proteins-bind-specifically-to-two-different-origins-of-replication-in%C3%A2-vitro
#16
Katarzyna I Kozłowska, Joanna Tymecka-Mulik, Grzegorz Węgrzyn
The O protein is a crucial factor initiating the DNA replication of lambdoid bacteriophage. Efficient DNA replication of Shiga toxin-converting phage is necessary for effective production of Shiga toxin - main virulence factor of STEC strains. We developed an improved protocol for overproduction, bacterial cell lysis and purification of λO protein. With use of this method we have also isolated O proteins of Stx-phage P27 and 933W that were never purified before. Purified proteins were tested for their DNA binding activity and revealed a sequence specific interactions...
November 5, 2016: Protein Expression and Purification
https://www.readbyqxmd.com/read/27822867/rapid-snp-detection-and-genotyping-of-bacterial-pathogens-by-pyrosequencing
#17
Kingsley K Amoako, Matthew C Thomas, Timothy W Janzen, Noriko Goji
Bacterial identification and typing are fixtures of microbiology laboratories and are vital aspects of our response mechanisms in the event of foodborne outbreaks and bioterrorist events. Whole genome sequencing (WGS) is leading the way in terms of expanding our ability to identify and characterize bacteria through the identification of subtle differences between genomes (e.g. single nucleotide polymorphisms (SNPs) and insertions/deletions). Modern high-throughput technologies such as pyrosequencing can facilitate the typing of bacteria by generating short-read sequence data of informative regions identified by WGS analyses, at a fraction of the cost of WGS...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27817960/multifaceted-immune-responses-and-protective-efficacy-elicited-by-a-recombinant-autolyzed-salmonella-expressing-flic-flagellar-antigen-of-f18-escherichia-coli
#18
Gayeon Won, John Hwa Lee
Porcine edema disease (ED) caused by F18(+) Shiga toxin 2e-producing Escherichia coli (STEC) has imposed significant economic losses in the swine industry worldwide, resulting in sudden deaths in post-weaned piglets. The flagellin protein of F18(+) STEC, a structural component of the flagellar filament, is a known virulence factor that mediates adhesion and invasion to porcine epithelial cells. In this study, Salmonella inactivated by the E lysis gene and expressing the flagellin (fliC) antigen was genetically engineered utilizing a plasmid (pMMP184) carrying an efficient heterologous antigen delivery system...
December 7, 2016: Vaccine
https://www.readbyqxmd.com/read/27810218/occurrence-genotyping-shiga-toxin-genes-and-associated-risk-factors-of-e-coli-isolated-from-dairy-farms-handlers-and-milk-consumers
#19
M A Awadallah, H A Ahmed, A M Merwad, M A Selim
The objectives of the current study were to determine the occurrence and genotypes of E. coli in dairy farms, workers and milk consumers and to evaluate risk factors associated with contamination of milk in dairy farms. Molecular characterization of shiga toxin associated genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) finger printing of E. coli from different sources were also studied. Paired milk samples and rectal swabs from 125 dairy cows, rectal swabs from 82 calves and hand swabs from 45 dairy workers from five dairy farms were collected...
November 2016: Veterinary Journal
https://www.readbyqxmd.com/read/27800426/prevalence-and-molecular-characterisation-of-shiga-toxin-producing-escherichia-coli-in-raw-milk-cheeses-from-lazio-region-italy
#20
Selene Marozzi, Paola De Santis, Sarah Lovari, Roberto Condoleo, Stefano Bilei, Rita Marcianò, Ziad Mezher
In recent years, the incidence of foodborne diseases caused by Shiga toxin-producing Escherichia coli (STEC) has increased globally. For this reason, within the specific regional control plan for the detection of STEC in food products in Italy, the presence of STEC in unpasteurised milk cheeses was investigated. In total, 203 samples obtained from March 2011 to December 2013 were analysed, with two standard methods (ISO 16654:2001 and ISO 13136:2012). Two strains of E. coli O157 were isolated (2/161, 1.2%) but did not carry any virulence-associated genes and 22 stx-positive samples (22/146, 15...
January 18, 2016: Italian Journal of Food Safety
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