T Shibata, H Sakai, F Nakamura, A Shioi, M Kuno
Effects of the extracellular Ca2+ concentration ([Ca2+]o) on whole cell membrane currents were examined in mouse osteoclastic cells generated from bone marrow/stromal cell coculture. The major resting conductance in the presence of 1 mm Ca2+ was mediated by a Ba2+-sensitive, inwardly rectifying K+ (IRK) current. A rise in -Ca2+-o (5-40 mM) inhibited the IRK current and activated an 4'4'-diisothiocyano-2,2'-stilbenedisulfonate (DIDS)-sensitive, outwardly rectifying Cl- (ORCl) current. The activation of the ORCl current developed slowly and needed higher [Ca2+]o than that required to inhibit the IRK current...
July 1, 1997: Journal of Membrane Biology