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https://www.readbyqxmd.com/read/28330336/postpartum-modern-contraceptive-use-and-associated-factors-in-northern-ethiopia
#1
Teklehaymanot Huluf Abraha, Alemayehu Shimeka Teferra, Abebaw Addis Gelagay
OBJECTIVES: Postpartum period is a critical period to address high unmet needs of family planning and to reduce the risks of closely spaced pregnancies. However, contraception during extended postpartum period has given less emphasis concerning contraceptive practice in Ethiopia. Therefore, this study was aimed to assess postpartum modern contraceptive use and associated factors among postpartum women in Northern Ethiopia. METHODS: A community based cross sectional study was done from March to April, 2015...
March 20, 2017: Epidemiology and Health
https://www.readbyqxmd.com/read/28330332/response-surface-methodology-based-optimization-of-production-media-and-purification-of-%C3%AE-galactosidase-in-solid-state-fermentation-by-fusarium-moniliforme-ncim-1099
#2
Sanjivani B Gajdhane, Prashant K Bhagwat, Padma B Dandge
Response surface methodology was used to enhance the production of α-galactosidase from Fusarium moniliforme NCIM 1099 in solid-state fermentation. Plackett-Burman design was employed for selection of critical media constituents which were optimized by central composite rotatable design. Wheat bran, peptone and FeSO4·7H2O were identified as significant medium components using PB design. Further CCRD optimized medium components as wheat bran; 4.62 μg, peptone; 315.42 μg, FeSO4·7H2O; 9.04 μg. RSM methodological optimization increased the enzyme production from 13...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330329/diversity-of-bacteria-and-archaea-in-the-rhizosphere-of-bioenergy-crop-jatropha-curcas
#3
Garima Dubey, Bharati Kollah, Vijay Kumar Gour, Arvind Kumar Shukla, Santosh Ranjan Mohanty
Plant-microbial interaction in rhizosphere plays vital role in shaping plant's growth and ecosystem function. Most of the rhizospheric microbial diversity studies are restricted to bacteria. In natural ecosystem, archaea also constitutes a major component of the microbial population. However, their diversity is less known compared to bacteria. Experiments were carried out to examine diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas (J. curcas). Samples were collected from three locations varying widely in the soil physico-chemical properties...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330321/antimicrobial-antibiofilm-and-microbial-barrier-properties-of-poly-%C3%AE%C2%B5-caprolactone-cloisite-30b-thin-films
#4
Snigdha Sajeendra Babu, Shiji Mathew, Nandakumar Kalarikkal, Sabu Thomas, Radhakrishnan E K
Development of antibacterial and antibiofilm surfaces is in high demand. In this study, nanocomposite of Poly (ε-caprolactone)/Cloisite 30B was prepared by the solvent casting method. The membranes were characterised by SEM, AFM, and FTIR. Evaluation of water uptake, antimicrobial, antibiofilm, and microbial barrier properties demonstrated a significant antimicrobial and antibiofilm activity against MTCC strain of Staphylococcus haemolyticus and strong biofilm positive Staphylococcus epidermidis of clinical origin at low clay concentrations...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330312/isolation-and-screening-of-l-asparaginase-free-of-glutaminase-and-urease-from-fungal-sp
#5
Kruthi Doriya, Devarai Santhosh Kumar
L-Asparaginase is a chemotherapeutic drug used in the treatment of acute lymphoblastic leukaemia (ALL), a malignant disorder in children. L-Asparaginase helps in removing acrylamide found in fried and baked foods that is carcinogenic in nature. L-Asparaginase is present in plants, animals and microbes. Various microorganisms such as bacteria, yeast and fungi are generally used for the production of L-asparaginase as it is difficult to obtain the same from plants and animals. L-Asparaginase from bacteria causes anaphylaxis and other abnormal sensitive reactions due to low specificity to asparagine...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330293/effect-of-reduced-plant-height-on-drought-tolerance-in-rice
#6
Asadollah Ahmadikhah, Amir Marufinia
Drought stress due to water deficit is a major problem of rice cultivation as a most drought-sensitive crop plant. A rice mutant line (MT58) was developed after mutagenesis of cv. Neda by ethyl methane sulfonate (EMS) and selected for dwarfism (18 cm shorter than Neda). The extent of its molecular changes relative to parental cultivar was assessed by SSR and ISSR markers, and the response of the line along with parental cultivar and another mutant line (MTA) to mild and severe water deficit, was evaluated in a field experiment...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330278/effects-of-the-culture-media-optimization-on-pectinase-production-by-enterobacter-sp-pstb-1
#7
M Purna Chandra Reddy, K V Saritha
In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330275/phytochemicals-of-moringa-oleifera-a-review-of-their-nutritional-therapeutic-and-industrial-significance
#8
REVIEW
Ramesh Kumar Saini, Iyyakkannu Sivanesan, Young-Soo Keum
Moringa oleifera Lam., also known as the 'drumstick tree,' is recognized as a vibrant and affordable source of phytochemicals, having potential applications in medicines, functional food preparations, water purification, and biodiesel production. The multiple biological activities including antiproliferation, hepatoprotective, anti-inflammatory, antinociceptive, antiatherosclerotic, oxidative DNA damage protective, antiperoxidative, cardioprotective, as well as folk medicinal uses of M. oleifera (MO) are attributed to the presence of functional bioactive compounds, such as phenolic acids, flavonoids, alkaloids, phytosterols, natural sugars, vitamins, minerals, and organic acids...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330257/generation-of-an-inducible-system-to-express-polo-like-kinase-cdc5-as-tap-fusion-protein-during-meiosis-in-saccharomyces-cerevisiae
#9
Rajni Vaid, Kamal Dev, Michael Lichten, Anuradha Sourirajan
Tandem affinity purification (TAP) is a highly efficient method for isolation of protein complexes from endogenous biological macromolecules. TAP system consists of dual affinity tags that facilitates the sequential purification of the desired proteins expressed at their low levels in vivo. Polo-like kinases (PLK) are serine/threonine protein kinases that are the crucial regulators of cell cycle. Cdc5, the solitary PLK in budding yeast Saccharomyces cerevisiae, has diverse array of targets in cell cycle. The present study was undertaken to construct an estrogen-inducible system for expression of Cdc5-TAP to isolate the substrates of Cdc5 during meiosis, particularly, pachytene stage of meiosis I...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330246/microbial-enzymes-industrial-progress-in-21st-century
#10
REVIEW
Rajendra Singh, Manoj Kumar, Anshumali Mittal, Praveen Kumar Mehta
Biocatalytic potential of microorganisms have been employed for centuries to produce bread, wine, vinegar and other common products without understanding the biochemical basis of their ingredients. Microbial enzymes have gained interest for their widespread uses in industries and medicine owing to their stability, catalytic activity, and ease of production and optimization than plant and animal enzymes. The use of enzymes in various industries (e.g., food, agriculture, chemicals, and pharmaceuticals) is increasing rapidly due to reduced processing time, low energy input, cost effectiveness, nontoxic and eco-friendly characteristics...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330216/potential-application-of-waste-from-castor-bean-ricinus-communis-l-for-production-for-xylanase-of-interest-in-the-industry
#11
Polyanna Nunes Herculano, Keila Aparecida Moreira, Raquel Pedrosa Bezerra, Tatiana Souza Porto, Cristina Maria de Souza-Motta, Ana Lúcia Figueiredo Porto
Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/citrate aqueous two-phase system (ATPS) were investigated. XY production was influenced by substrate amount (5-10 g), initial moisture (15-35 %), pH (4.0-6.0) and temperature (25-35 °C), obtaining the maximum activity of 29,085 ± 1808 U g ds(-1) using 5.0 g of substrate with initial moisture of 15 % at 25 °C and pH 6...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330198/pretreatment-and-enzymatic-process-modification-strategies-to-improve-efficiency-of-sugar-production-from-sugarcane-bagasse
#12
Siddhartha Pal, Shereena Joy, Kalpana D Trimukhe, Pramod S Kumbhar, Anjani J Varma, Sasisanker Padmanabhan
Pretreatment and enzymatic hydrolysis play a critical role in the economic production of sugars and fuels from lignocellulosic biomass. In this study, we evaluated diverse pilot-scale pretreatments and different post-pretreatment strategies for the production of fermentable sugars from sugarcane bagasse. For the pretreatment of bagasse at pilot-scale level, steam explosion without catalyst and combination of sulfuric and oxalic acids at low and high loadings were used. Subsequently, to enhance the efficiency of enzymatic hydrolysis of the pretreated bagasse, three different post-pretreatment process schemes were investigated...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330193/a-rapid-method-for-isolation-of-genomic-dna-from-food-borne-fungal-pathogens
#13
S Umesha, H M Manukumar, Sri Raghava
Food contaminated with fungal pathogens can cause extremely harmful effects to human even when present in low concentrations. Researchers now pay more attention towards rapid DNA extraction for the quick screening, which is highly demanded in diverse research field. Molecular description of many fungal species is identified by different molecular characteristics. Hence, the efficient isolation of genomic DNA and amplification using PCR is a prerequisite for molecular characterization. Here, we used an improved Sodium dodecyl sulfate-Cetyltrimethyl ammonium bromide-Chloroform-isoamyl alcohol method by combining Sodium dodecyl sulfate with cetyl methylammonium bromide without addition of proteinase K, RNase K, and β-mercaptoethanol...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330178/emergence-of-mixed-infection-of-beijing-non-beijing-strains-among-multi-drug-resistant-mycobacterium-tuberculosis-in-pakistan
#14
Samar Mustafa, Hasnain Javed, Jawairia Hashmi, Nazia Jamil, Zarfishan Tahir, Abdul Majeed Akhtar
Tuberculosis (TB) remains as one of the deadliest diseases after HIV globally with 95 % of deaths confined to low-and-middle income countries. Pakistan is fifth among the 22 high-burden TB countries with the incidence rate of 230/100,000 persons, however, studies related to prevalent Mycobacterium tuberculosis strains and their spread, drug resistance pattern and evolutionary genetics are inadequate. The present study was undertaken to highlight the circulation of M. tuberculosis strains causing drug resistant TB in our community by targeting the molecular marker IS6110 and then characterization of these strains as Beijing and Non-Beijing genotypes...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330176/molecular-dissection-of-pathway-components-unravel-atisine-biosynthesis-in-a-non-toxic-aconitum-species-a-heterophyllum-wall
#15
Varun Kumar, Nikhil Malhotra, Tarun Pal, Rajinder Singh Chauhan
Aconitum heterophyllum is an important component for various herbal drug formulations due to the occurrence of non-toxic aconites including marker compound, atisine. Despite huge pharmacological potential, the reprogramming of aconites production is limited due to lack of understanding on their biosynthesis. To address this problem, we have proposed here the complete atisine biosynthetic pathway for the first time connecting glycolysis, MVA/MEP, serine biosynthesis and diterpene biosynthetic pathways. The transcript profiling revealed phosphorylated pathway as a major contributor towards serine production in addition to repertoire of genes in glycolysis (G6PI, PFK, ALD and ENO), serine biosynthesis (PGDH and PSAT) and diterpene biosynthesis (KO and KH) sharing a similar pattern of expression (2-4-folds) in roots compared to shoots vis-à-vis atisine content (0-0...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330167/genetic-characterization-of-moroccan-and-the-exotic-bread-wheat-cultivars-using-functional-and-random-dna-markers-linked-to-the-agronomic-traits-for-genomics-assisted-improvement
#16
Fatima Henkrar, Jamal El-Haddoury, Hassan Ouabbou, Najib Bendaou, Sripada M Udupa
Genetic characterization, diversity analysis and estimate of the genetic relationship among varieties using functional and random DNA markers linked to agronomic traits can provide relevant guidelines in selecting parents and designing new breeding strategies for marker-assisted wheat cultivar improvement. Here, we characterize 20 Moroccan and 19 exotic bread wheat (Triticum aestivum L.) cultivars using 47 functional and 7 linked random DNA markers associated with 21 loci of the most important traits for wheat breeding...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330163/probing-an-optimal-class-distribution-for-enhancing-prediction-and-feature-characterization-of-plant-virus-encoded-rna-silencing-suppressors
#17
Abhigyan Nath, Karthikeyan Subbiah
To counter the host RNA silencing defense mechanism, many plant viruses encode RNA silencing suppressor proteins. These groups of proteins share very low sequence and structural similarities among them, which consequently hamper their annotation using sequence similarity-based search methods. Alternatively the machine learning-based methods can become a suitable choice, but the optimal performance through machine learning-based methods is being affected by various factors such as class imbalance, incomplete learning, selection of inappropriate features, etc...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330145/enhanced-xylitol-production-using-immobilized-candida-tropicalis-with-non-detoxified-corn-cob-hemicellulosic-hydrolysate
#18
Tatyaso Yewale, Shruti Panchwagh, Srinivasan Rajagopalan, Pradip B Dhamole, Rishi Jain
This study reports an industrially applicable non-sterile xylitol fermentation process to produce xylitol from a low-cost feedstock like corn cob hydrolysate as pentose source without any detoxification. Different immobilization matrices/mediums (alginate, polyvinyl alcohol, agarose gel, polyacrylamide, gelatin, and κ-carrageenan) were studied to immobilize Candida tropicalis NCIM 3123 cells for xylitol production. Amongst this calcium alginate, immobilized cells produced maximum amount of xylitol with titer of 11...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330132/an-efficient-and-cost-effective-method-for-dna-extraction-from-athalassohaline-soil-using-a-newly-formulated-cell-extraction-buffer
#19
Avinash Narayan, Kunal Jain, Amita R Shah, Datta Madamwar
The present study describes the rapid and efficient indirect lysis method for environmental DNA extraction from athalassohaline soil by newly formulated cell extraction buffer. The available methods are mostly based on direct lysis which leads to DNA shearing and co-extraction of extra cellular DNA that influences the community and functional analysis. Moreover, during extraction of DNA by direct lysis from athalassohaline soil, it was observed that, upon addition of poly ethylene glycol (PEG), isopropanol or absolute ethanol for precipitation of DNA, salt precipitates out and affecting DNA yield significantly...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330123/microbial-degradation-of-low-density-polyethylene-ldpe-by-aspergillus-clavatus-strain-jask1-isolated-from-landfill-soil
#20
Anudurga Gajendiran, Sharmila Krishnamoorthy, Jayanthi Abraham
Polythene and plastic waste are found to accumulate in the environment, posing a major ecological threat. They are found to be considered non-degradable, once it enters the environment it has been found to remain there indefinitely. However, significant attention has been placed on biodegradable polymer, identification of microbes with degradative potential on plastic material. The aim of the present investigation was to biodegrade low-density polyethylene (LDPE) using potential fungi isolated from landfill soil...
June 2016: 3 Biotech
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