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https://www.readbyqxmd.com/read/28238632/the-cationic-liposomal-adjuvants-caf01-and-caf09-formulated-with-the-major-outer-membrane-protein-elicit-robust-protection-in-mice-against-a-chlamydia-muridarum-respiratory-challenge
#1
Sukumar Pal, Delia F Tifrea, Frank Follmann, Peter Andersen, Luis M de la Maza
Two cationic liposomal adjuvants CAF01 and CAF09 were formulated with the native or the recombinant Chlamydia muridarum major outer membrane protein (nMOMP and rMOMP). BALB/c mice were immunized with the four vaccine formulations using the subcutaneous followed by the intranasal (i.n.) routes. As positive controls mice were inoculated i.n. with live C. muridarum and negative controls received i.n. minimal essential medium (MEM). Four weeks after the last immunization mice were challenged i.n. with 10(4) inclusion forming units (IFU) of C...
February 23, 2017: Vaccine
https://www.readbyqxmd.com/read/28228375/is-it-time-to-reinvent-basic-cell-culture-medium
#2
Turney J McKee, Svetlana V Komarova
The success of in vitro experiments depends largely on the quality of the cell culture media. Four synthetic media formulations, Dulbecco's Modified Minimum Essential Media (DMEM), RPMI 1640, Minimum Essential Media and its alpha modification (MEM), and Medium 199 (M199) are used in ~90% of published in vitro studies. We compared the levels of electrolytes and carbohydrates in these media formulations to physiological values in plasma and cerebrospinal fluid. The most commonly used media, DMEM and RPMI 1640, largely deviate from physiological levels of glucose...
February 22, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28227848/motion-noise-cancellation-in-seismocardiogram-of-ambulant-subjects-with-dual-sensors
#3
Chenxi Yang, Negar Tavassolian, Chenxi Yang, Negar Tavassolian, Negar Tavassolian, Chenxi Yang
This paper presents a dual-sensor method of extracting seismocardiographic (SCG) data from moving adult subjects using chest-worn wireless MEMS accelerometers. A digital signal processing (DSP) system including a normalized least means square (NLMS) adaptive filter is designed and tested in MATLAB. Data results from 10 subjects indicate a detection rate of 98.72% which outperforms our previously-proposed single-sensor scheme. Various sensor positons and possible failure mechanisms are also investigated to further evaluate the system performance...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227817/polyimide-based-magnetic-microactuators-for-biofouling-removal
#4
Qi Yang, Tran Nguyen, Chunan Liu, Jacob Miller, Jeffrey F Rhoads, Jacqueline Linnes, Hyowon Lee, Qi Yang, Tran Nguyen, Chunan Liu, Jacob Miller, Jeffrey F Rhoads, Jacqueline Linnes, Hyowon Lee, Jacqueline Linnes, Chunan Liu, Tran Nguyen, Jacob Miller, Jeffrey F Rhoads, Hyowon Lee, Qi Yang
Here we report on the development of novel polyimide-based flexible magnetic actuators for improving hydrocephalus shunts. The static and dynamic mechanical responses of the thin-film magnetic microdevices were quantitatively measured. The bacteria-removing capabilities of the microfabricated devices were also evaluated. Although additional evaluations are necessary, the preliminary results show promising potential for combatting bacteria-induced biofouling. Lastly, the thin-film microdevices are integrated into a single-pore silicone catheter to demonstrate a proof-of-concept, MEMS-enabled self-clearing, smart catheter...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227508/design-and-evaluation-of-a-low-cost-intracranial-pressure-monitoring-system
#5
Ranjani Sampath Kumaran, Bradly Greger, Jennifer Blain Christen, Ranjani Sampath Kumaran, Bradly Greger, Jennifer Blain Christen, Jennifer Blain Christen, Ranjani Sampath Kumaran, Bradly Greger
One of the most life-threatening neural conditions is elevated intracranial pressure (ICP); it is associated with ischemia and poor short- and long-term outcomes. Currently, monitoring systems that accurately measure ICP are either highly invasive or inaccurate. This work explores the design and evaluation of an epidural intracranial pressure monitoring system for low-cost, minimally invasive detection. Our goal is to develop a monitoring system that could also be integrated with an electrocorticography (ECoG) system...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227480/detection-of-atrial-fibrillation-with-seismocardiography
#6
Mikko Pankaala, Tero Koivisto, Olli Lahdenoja, Tuomas Kiviniemi, Antti Saraste, Tuija Vasankari, Juhani Airaksinen, Mikko Pankaala, Tero Koivisto, Olli Lahdenoja, Tuomas Kiviniemi, Antti Saraste, Tuija Vasankari, Juhani Airaksinen, Tero Koivisto, Olli Lahdenoja, Tuomas Kiviniemi, Antti Saraste, Tuija Vasankari, Mikko Pankaala, Juhani Airaksinen
In this paper we study the feasibility of seismocardiography (SCG) for the detection of Atrial Fibrillation (AF). In this preclinical study, data acquired from one patient having paroxysmal AF (no other heart diseases) is used to introduce specific changes in SCG signal due to AF. Observed changes and phenomena create a foundation for the development of SCG-based AF detection algorithms. SCG data was recorded from the sternum of an AF patient in dorso-ventral direction while at rest in a supine position using a three-axis high precision MEMS accelerometer simultaneously with a one-lead ECG...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226919/an-online-recursive-autocalibration-of-triaxial-accelerometer
#7
Lin Ye, Steven W Su, Dong Lei, Hung T Nguyen, Lin Ye, Steven W Su, Dong Lei, Hung T Nguyen, Steven W Su, Hung T Nguyen, Lin Ye, Dong Lei
In this paper, we proposed a novel method for autocalibration of triaxial Micro-Electro-Mechanical systems (MEMS) accelerometer that does not require any sophisticated laboratory facilities. In particular, this method is an online calibration method which can be conveniently implemented with the accuracy of MEMS accelerometer being significantly improved. The procedure exploits the fact that the output vector of the accelerometer must match the local gravity in static state condition. To achieve online calibration, the model as well as the cost function are linearized at the beginning, and an online recursive method is then utilized to identify the unknown parameters and remove the bias caused by linearization...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226918/gyrocardiography-a-new-non-invasive-approach-in-the-study-of-mechanical-motions-of-the-heart-concept-method-and-initial-observations
#8
Mojtaba Jafari Tadi, Eero Lehtonen, Mikko Pankaala, Antti Saraste, Tuija Vasankari, Mika Teras, Tero Koivisto, Mojtaba Jafari Tadi, Eero Lehtonen, Mikko Pankaala, Antti Saraste, Tuija Vasankari, Mika Teras, Tero Koivisto, Eero Lehtonen, Mojtaba Jafari Tadi, Mika Teras, Antti Saraste, Tuija Vasankari, Mikko Pankaala, Tero Koivisto
The pumping action of the heart is performed by contraction of the myocardium fibers. We present a non-invasive technique named gyrocardiography (GCG) that comprises a sensor of angular motion, gyroscope, configured to obtain three-dimensional angular velocity signals. A tri-axial micro electromechanical (MEMS) gyroscope sensor was attached to the surface of the chest to obtain gyrocardiogram. Color-coded Doppler tissue imaging (DTI) was recorded simultaneously and synchronized with the GCG in an off-line analysis...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226535/probing-the-micromechanics-of-the-fastest-growing-plant-cell-the-pollen-tube
#9
Naveen Shamsudhin, Huseyin Baris Atakan, Nino Laubli, Hannes Vogler, Chengzhi Hu, Abu Sebastian, Ueli Grossniklaus, Bradley J Nelson, Naveen Shamsudhin, Huseyin Baris Atakan, Nino Laubli, Hannes Vogler, Chengzhi Hu, Abu Sebastian, Ueli Grossniklaus, Bradley J Nelson, Hannes Vogler, Ueli Grossniklaus, Nino Laubli, Bradley J Nelson, Naveen Shamsudhin, Huseyin Baris Atakan, Abu Sebastian, Chengzhi Hu
The pollen tube is a fast growing cellular protrusion that plays a key role in the reproductive process of flowering plants. It serves as an important model for studying cellular morphogenesis, anisotropic growth mechanisms, and cellular signaling in the plant sciences. The anisotropic growth of pollen tubes is driven by a finely tuned control of the intracellular turgor pressure and the extensibility of the cell wall. To decipher this internal feedback loop and mathematically model the growth process, a quantitative understanding of the mechanical properties of the cell wall is crucial, in addition to biochemical investigations...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226456/hydrogel-reinforced-polypyrrole-electroactuator
#10
Yongchen Wang, Bingxi Yan, Yu Wu, Liang Guo, Yongchen Wang, Bingxi Yan, Yu Wu, Liang Guo, Yongchen Wang, Liang Guo, Bingxi Yan, Yu Wu
Robust electroactuators that are lightweight, power-efficient and capable of generating high forces at a low actuation voltage are of great demand in the design of micro-electromechanical systems (MEMS) for biomedical applications. In this work, electroactive composite films of alginate hydrogel and polypyrrole/polyethylene glycol (PPy/PEG) are synthesized and characterized as actuators for driving an implantable insulin micropump. A porous and flexible alginate hydrogel back layer is used to mechanically reinforce the PPy/PEG film by enhancing the actuator's resistance to its generated tension but without increase of its overall rigidness...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28225450/behavioral-economic-incentives-to-improve-adherence-to-antiretroviral-medication
#11
Sebastian Linnemayr, Chad Stecher, Barbara Mukasa
OBJECTIVE: Fixed incentives have been largely unsuccessful in improving adherence to antiretroviral medication. Therefore, we evaluate whether small incentives based on behavioral economic theory can increase adherence to antiretroviral medication among treatment-mature adults in Kampala, Uganda. DESIGN: A randomized control trial design tests whether providing small incentives based on either attending timely clinic visits (intervention group 1) or achieving high medication adherence (intervention group 2) can increase antiretroviral adherence...
March 13, 2017: AIDS
https://www.readbyqxmd.com/read/28211993/recent-lab-on-chip-developments-for-novel-drug-discovery
#12
REVIEW
Nauman Khalid, Isao Kobayashi, Mitsutoshi Nakajima
Microelectromechanical systems (MEMS) and micro total analysis systems (μTAS) revolutionized the biochemical and electronic industries, and this miniaturization process became a key driver for many markets. Now, it is a driving force for innovations in life sciences, diagnostics, analytical sciences, and chemistry, which are called 'lab-on-a-chip, (LOC)' devices. The use of these devices allows the development of fast, portable, and easy-to-use systems with a high level of functional integration for applications such as point-of-care diagnostics, forensics, the analysis of biomolecules, environmental or food analysis, and drug development...
February 17, 2017: Wiley Interdisciplinary Reviews. Systems Biology and Medicine
https://www.readbyqxmd.com/read/28208327/issues-associated-with-galilean-invariance-on-a-moving-solid-boundary-in-the-lattice-boltzmann-method
#13
Cheng Peng, Nicholas Geneva, Zhaoli Guo, Lian-Ping Wang
In lattice Boltzmann simulations involving moving solid boundaries, the momentum exchange between the solid and fluid phases was recently found to be not fully consistent with the principle of local Galilean invariance (GI) when the bounce-back schemes (BBS) and the momentum exchange method (MEM) are used. In the past, this inconsistency was resolved by introducing modified MEM schemes so that the overall moving-boundary algorithm could be more consistent with GI. However, in this paper we argue that the true origin of this violation of Galilean invariance (VGI) in the presence of a moving solid-fluid interface is due to the BBS itself, as the VGI error not only exists in the hydrodynamic force acting on the solid phase, but also in the boundary force exerted on the fluid phase, according to Newton's Third Law...
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28207393/optimization-of-the-close-to-carrier-phase-noise-in-a-cmos-mems-oscillator-using-a-phase-tunable-sustaining-amplifier
#14
Guillermo Sobreviela, Martin Riverola, Francesc Canals, Arantxa Del Monte, Nuria Beumala
In this paper, the phase noise of a 24-MHz CMOSMEMS oscillator with zero-level vacuum package is studied. We characterize and analyze the nonlinear regime of each one of the modules that compose the oscillator (CMOS sustaining-amplifier and MEMS resonator). As we show, the presented resonator exhibits a high nonlinear behavior. Such fact is exploited as a mechanism to stabilize the oscillation amplitude, allowing to maintain the sustaining-amplifier working in the linear regime. Consequently, the nonlinear resonator becomes the main closeto- carrier phase noise source...
February 13, 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28207392/temperature-compensation-of-silicon-lam%C3%A3-resonators-using-etch-holes-theory-and-design-methodology
#15
Luca Luschi, Giuseppe Iannaccone, Francesco Pieri
We present a new approach to the temperature compensation of MEMS Lamé resonators, based on the combined effect of the doping concentration and of the geometry of etch holes on the equivalent temperature coefficients of silicon. To this purpose, we develop and validate an analytical model which describes the effect of etch holes on the temperature stability of Lamé resonators through comparison with experiments available in the literature and FEM simulations. We show that two interesting regions of the design space for Lamé resonators exist, where a cancellation of the first-order temperature coefficient of the resonance frequency is possible: [100]-oriented silicon with n-doping of 2...
February 9, 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28205023/low-serum-culture-with-novel-medium-promotes-maxillary-mandibular-bone-marrow-stromal-cell-proliferation-and-osteogenic-differentiation-ability
#16
Fumio Suehiro, Masakazu Ishii, Izumi Asahina, Hiroshi Murata, Masahiro Nishimura
OBJECTIVES: The purpose of this study was to evaluate the effect of low-serum STK2 medium on the isolation and osteogenic differentiation of human maxillary/mandibular bone marrow stromal cells (MBMSCs). MATERIALS AND METHODS: Human MBMSCs were obtained from patients undergoing dental implant treatment. These cells were cultured in serum-free medium or STK2 medium containing 1  % fetal bovine serum (low-serum) or α-MEM containing 10  % fetal bovine serum (control)...
February 16, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28203427/potency-of-parenteral-antimicrobials-including-ceftolozane-tazobactam-against-nosocomial-respiratory-tract-pathogens-considerations-for-empiric-and-directed-therapy
#17
Christina A Sutherland, David P Nicolau
BACKGROUND: Empiric therapy decisions are predicated on knowledge of both the epidemiology and antimicrobial susceptibility of the probable infecting pathogen(s). The objective of this study was to evaluate the microbial distribution and phenotypic profiles of nosocomial respiratory isolates collected from multiple US hospitals and assess the clinical utility of various monotherapy and combination regimens. METHODS: Hospitals provided consecutive non-duplicate adult inpatients Gram-negative nosocomial respiratory isolates from cultures received ≥48 h after hospital admission...
January 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28198874/mems-tunable-vcsels-using-2d-high-contrast-gratings
#18
Pengfei Qiao, Kun Li, Kevin T Cook, Connie J Chang-Hasnain
We demonstrate the room-temperature operation of a two-dimensional (2D) high-contrast grating (HCG) vertical-cavity surface-emitting laser (VCSEL) at 1080 nm. To the best of our knowledge, this is the first tunable electrically pumped surface-emitting laser using a 2D HCG. Our theory successfully explains the mechanism of broadband ultrahigh reflection of 2D HCGs. Our monolithic integrated laser exhibits single-mode output power above 0.68 mW under continuous-wave operation. Wavelength tunability is demonstrated via microelectromechanical system-controlled voltage...
February 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28198631/robust-alginate-catechol-polydopamine-free-standing-mem-branes-obtained-from-the-water-air-interface
#19
Florian Ponzio, Vincent Le Houérou, Spyridon Zafeiratos, Christian Gauthier, Tony Garnier, Loic Jierry, Vincent Ball
The formation of polydopamine composite membranes at the water/air interface using different chemical strategies is reported. The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air inter-face. Based on these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsive and self-healing properties...
February 15, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28197987/effects-of-frutalin-on-early-follicle-morphology-ultrastructure-and-gene-expression-in-cultured-goat-ovarian-cortical-tissue
#20
Maria A A Soares, José J N Costa, Gisvani L Vasconcelos, Regislane P Ribeiro, José C Souza, André L C Silva, Robert Van den Hurk, José R V Silva
Frutalin is a galactose-binding lectin that has an irreversible cytotoxic effect on HeLa cervical cancer cells, by inducing apoptosis and inhibiting cell proliferation. It was previously shown that after in vitro incubation, frutalin is internalized into HeLa cells nucleus, which indicates that frutalin apoptosis-inducing activity might be linked with its nuclear localization. Considering that drugs commonly used for cancer treatment have a deleterious effect on germ cells, the aim of this study was to evaluate the effect of frutalin on the activation, survival, ultrastructure and gene expression in follicles cultured within ovarian tissue...
February 15, 2017: Histology and Histopathology
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