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Conditioned media of mesenchymal stem cell

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https://www.readbyqxmd.com/read/29783120/canine-mesenchymal-stem-cells-treated-with-tnf-%C3%AE-and-ifn-%C3%AE-enhance-anti-inflammatory-effects-through-the-cox-2-pge-2-pathway
#1
Hye-Mi Yang, Woo-Jin Song, Qiang Li, Su-Yeon Kim, Hyeon-Jin Kim, Min-Ok Ryu, Jin-Ok Ahn, Hwa-Young Youn
Mesenchymal stem cells (MSCs) have been used in studies on treatment of various diseases, and their application to immune-mediated diseases has garnered interest. Various methods for enhancing the immunomodulation effect of human MSCs have been used; however, similar approaches for canine MSCs are relatively unexplored. Accordingly, we evaluated immunomodulatory effects and mechanisms in canine MSCs treated with TNF-α and IFN-γ. Lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were incubated with the conditioned media (CM) from canine MSCs for 48 h...
May 14, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29770732/characterization-of-canine-adipose-derived-mesenchymal-stromal-stem-cells-in-serum-free-medium
#2
Zhuoming Liu, Rudell Screven, Lynne Boxer, Michael Myers, Lax Devireddy
In this manuscript, we report on the development of a defined serum-free medium capable of supporting the culture expansion of mesenchymal stromal/stem cells (MSCs) from canine adipose tissue (canine Ad-MSCs). The potential benefits of serum-free media can only be utilized if cells cultured in serum-free media maintain the same functional characteristics as cells cultured in serum-containing media. Therefore, we analyze the characteristics of canine Ad-MSCs cultured in this serum-free medium or in serum-containing medium through evaluation of growth kinetics, clonogenic capacity, senescence, and differentiation capacity...
May 17, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29765409/a-novel-secretory-vesicle-from-deer-antlerogenic-mesenchymal-stem-cell-conditioned-media-damsc-cm-promotes-tissue-regeneration
#3
Minkoo Seo, Jin-Chul Kim, Hyung-Ki Kim, Eun Wook Choi, Suyeong Jeong, Ki Chang Nam, Mihue Jang
Multipotent stem cells have the capacity to generate terminally differentiated cell types of each lineage; thus, they have great therapeutic potential for a wide variety of diseases. The most widely available stem cells are derived from human tissues, and their use for therapeutic application is limited by their high cost and low productivity. Herein, we report that conditioned media of mesenchymal stem cells (MSCs) isolated from deer antlers enhanced tissue regeneration through paracrine action via a combination of secreted growth factors and cytokines...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29760942/induction-of-chondrogenesis-of-human-placenta-derived-mesenchymal-stem-cells-via-heparin-grafted-human-fibroblast-derived-matrix
#4
Yong Kwan Noh, Ping Du, Avelino Dos Santos Da Costa, Kwideok Park
Background: Formation of mature and functional articular cartilage is still challenging in cartilage tissue engineering. This study investigates the potential of using heparin-grafted decellularized extracellular matrix (ECM) as a novel growth factor delivery platform towards human placenta-derived mesenchymal stem cells (hPMSCs) chondrogenic differentiation. Human fibroblast-derived extracellular matrix (hFDM) is naturally obtained from in vitro-cultured human lung fibroblasts via a mild decellularization process...
2018: Biomaterials Research
https://www.readbyqxmd.com/read/29757659/bone-marrow-derived-mesenchymal-stem-cells-promoted-cutaneous-wound-healing-by-regulating-keratinocyte-migration-via-the-%C3%AE-2-adrenergic-receptor-signaling
#5
Jiahui Huo, Sujing Sun, Zhijun Geng, Wei Sheng, Runkai Chen, Kui Ma, Xiaoyan Sun, Xiaobing Fu
Mesenchymal stem cells (MSCs) play an important role in the cutaneous wound healing; however, the functional mechanisms involved in the healing process are poorly understood. A series of studies indicate that keratinocytes which migrate into the wound bed rely on an epithelial-mesenchymal transition (EMT)-like process to initiate re-epithelialization. We therefore examined whether bone marrow-derived MSCs (BMSCs) could affect biological behavior and induce EMT-like characteristics in the human epidermal keratinocytes (HEKs) and in the immortalized human keratinocyte cell line HaCaT cells; and we investigated the signaling pathways of BMSC-mediated phenotypic changes...
May 14, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29752083/how-cell-culture-conditions-affect-the-microstructure-and-nanomechanical-properties-of-extracellular-matrix-formed-by-immortalized-human-mesenchymal-stem-cells-an-experimental-and-modelling-study
#6
Pengfei Duan, Ria Toumpaniari, Simon Partridge, Mark A Birch, Paul G Genever, Steve J Bull, Kenneth W Dalgarno, Andrew W McCaskie, Jinju Chen
This paper presents an investigation of how different culture media (i.e. basal and osteogenic media) affect the nanomechanical properties and microstructure of the mineralized matrix produced by the human mesenchymal stem cell line Y201, from both an experimental and theoretical approach. A bone nodule (i.e. mineralized matrix) cultured from basal medium shows a more anisotropic microstructure compared to its counterpart cultured from an osteogenic medium. As confirmed by finite element simulations, this anisotropic microstructure explains the bimodal distribution of the corresponding mechanical properties very well...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29751774/changes-in-phenotype-and-differentiation-potential-of-human-mesenchymal-stem-cells-aging-in-vitro
#7
Yueh-Hsun Kevin Yang, Courtney R Ogando, Carmine Wang See, Tsui-Yun Chang, Gilda A Barabino
BACKGROUND: Adult mesenchymal stem cells (MSCs) hold great promise for regenerative medicine because of their self-renewal, multipotency, and trophic and immunosuppressive effects. Due to the rareness and high heterogeneity of freshly isolated MSCs, extensive in-vitro passage is required to expand their populations prior to clinical use; however, senescence usually accompanies and can potentially affect MSC characteristics and functionality. Therefore, a thorough characterization of the variations in phenotype and differentiation potential of in-vitro aging MSCs must be sought...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29744869/isolation-and-identification-of-chemotherapy-enriched-sphere-forming-cells-from-a-patient-with-gastric-cancer
#8
Vahid Bagheri, Bahram Memar, Ramezan Behzadi, Mohsen Aliakbarian, Ali Jangjoo, Mostafa Mehrabi Bahar, Samaneh Talebi, Mehran Gholamin, Mohammad R Abbaszadegan
Gastric cancer (GC) is the third and fifth cause of cancer-associated mortality for men and women throughout the world, respectively. Despite the use of surgery and chemotherapy for GC therapy, there are no efficient therapeutic protocols for it to date. Cancer stem cells (CSCs) due to their pivotal role in tumor initiation, growth, progression, invasion, distant metastasis, recurrence and resistance to anticancer drugs are very appealing targets for cancer therapies. Here, we isolated and identified CSCs from a chemotherapy-treated patient...
May 10, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29737535/neural-differentiation-of-human-adipose-derived-mesenchymal-stem-cells-induced-by-glial-cell-conditioned-media
#9
Debora Lo Furno, Giuliana Mannino, Rosario Giuffrida, Elisa Gili, Carlo Vancheri, Maria S Tarico, Rosario E Perrotta, Rosalia Pellitteri
Adipose-derived mesenchymal stem cells (ASCs) may transdifferentiate into cells belonging to mesodermal, endodermal, and ectodermal lineages. The aim of this study was to verify whether a neural differentiation of ASCs could be induced by a conditioned medium (CM) obtained from cultures of olfactory ensheathing cells (OECs) or Schwann cells (SCs). ASCs were isolated from the stromal vascular fraction of adipose tissue and expanded for 2-3 passages. They were then cultured in OEC-CM or SC-CM for 24 hr or 7 days...
May 8, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29684675/fak-and-yap-taz-dependent-mechanotransduction-pathways-are-required-for-enhanced-immunomodulatory-properties-of-adipose-derived-mesenchymal-stem-cells-induced-by-aligned-fibrous-scaffolds
#10
S Wan, X Fu, Y Ji, M Li, X Shi, Y Wang
Adipose-derived mesenchymal stem cells (ASCs) represent an excellent candidate for tissue engineering due to their multipotency and paracrine functions. Physical cues provided by the scaffolds have been shown to direct a variety of cellular behaviors of ASCs; however, their effects on the paracrine functions of ASCs, especially immunomodulatory functions, remain largely unexplored. Thus, PLLA electrospun fibrous scaffolds, with fibers oriented either randomly or aligned, were used as a model to investigate the effects of fiber orientation on the immunomodulatory paracrine secretion of ASCs...
April 16, 2018: Biomaterials
https://www.readbyqxmd.com/read/29671652/effects-of-educated-monocytes-with-xenogeneic-mesenchymal-stem-cell-derived-conditioned-medium-in-a-mouse-model-of-chronic-asthma
#11
Hadi Esmaili Gourvarchin Galeh, Seyyed Meysam Abtahi Froushani, Nahideh Afzale Ahangaran, Siamak Naji Hadai
BACKGROUND: This study was conducted to determine the potential of the monocytes educated with rat bone marrow mesenchymal stem cell-derived conditioned medium (MCM) in ameliorating animal model of asthma. METHODS: Chronic asthma was induced in the BALB/c mice using ovalbumin (OVA) sensitization. The monocytes were isolated from blood of mice and supplemented with 50% MCM or negative control media. After 24 h, the cells were designated as "non-educated or educated"...
April 19, 2018: Immunological Investigations
https://www.readbyqxmd.com/read/29623134/generating-chondromimetic-mesenchymal-stem-cell-spheroids-by-regulating-media-composition-and-surface-coating
#12
BanuPriya Sridharan, Amy D Laflin, Michael S Detamore
INTRODUCTION: Spheroids of mesenchymal stem cells (MSCs) in cartilage tissue engineering have been shown to enhance regenerative potential owing to their 3D structure. In this study, we explored the possibility of priming spheroids under different media to replace the use of inductive surface coatings for chondrogenic differentiation. METHODS: Rat bone marrow-derived MSCs were organized into cell spheroids by the hanging drop technique and subsequently cultured on hyaluronic acid (HA) coated or non-coated well plates under different cell media conditions...
April 2018: Cellular and Molecular Bioengineering
https://www.readbyqxmd.com/read/29596344/treatment-of-arsenite-intoxication-induced-peripheral-vasculopathy-with-mesenchymal-stem-cells
#13
Yi-Hung Chiang, Chai-Chin Lin, Yen-Chung Chen, Oscar K Lee
Arsenite (As), a notorious toxic metal, is ubiquitously distributed in the earth and poses a serious threat to human health. Histopathological lesions of As intoxication are known as thromboangiitis obliterans, which are resistant to current treatment and often lead to lower limb amputation. In this study, we attempt to find that treatment with mesenchymal stem cells (MSCs) may be effective for As-induced vasculopathy. We first conducted an in vitro study with a co-culture system containing human MSCs and human umbilical vein endothelial cells (HUVECs) and treated individual and co-cultured cells with various concentrations of arsenite...
March 29, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29590646/human-glioblastoma-derived-mesenchymal-stem-cell-to-pericytes-transition-and-angiogenic-capacity-in-glioblastoma-microenvironment
#14
Dongye Yi, Wei Xiang, Qing Zhang, Yongcun Cen, Qing Su, Fangcheng Zhang, Yinping Lu, Hongyang Zhao, Peng Fu
BACKGROUND/AIMS: Tumor vascular formation and maintenance are crucial events in glioblastoma development. Mesenchymal stem cells (MSCs) have been shown to differentiate into pericytes and contribute to neovascularization in the glioma microenvironment. Moreover, glioblastoma-derived mesenchymal stem cells (gb-MSCs), which consist of CD90-MSCs and CD90+MSCs, are a subpopulation of MSCs that are more active in glioma vascularization. However, the functions of gb-MSCs and the microRNA (miRNA) modifications in the glioblastoma microenvironment have not yet been fully elucidated...
March 22, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29555441/pro-angiogenic-impact-of-sdf-1%C3%AE-gene-activated-collagen-based-scaffolds-in-stem-cell-driven-angiogenesis
#15
Ashang Luwang Laiva, Rosanne M Raftery, Michael B Keogh, Fergal J O'Brien
Ensuring an adequate angiogenic response during wound healing is a prevailing clinical challenge in biomaterials science. To address this, we aimed to develop a pro-angiogenic gene-activated scaffold (GAS) that could activate MSCs to produce paracrine factors and influence angiogenesis and wound repair. A non-viral polyethyleneimine (PEI) nanoparticles carrying a gene encoding for stromal derived factor-1 alpha (SDF-1α) was combined with a collagen-chondroitin sulfate scaffold to produce the GAS. The ability of this platform to enhance the angiogenic potential of mesenchymal stem cells (MSCs) was then assessed...
March 17, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29554498/an-insight-into-the-role-of-magnesium-in-the-immunomodulatory-properties-of-mesenchymal-stem-cells
#16
Fabiana da Silva Lima, Amanda Batista da Rocha Romero, Araceli Hastreiter, Amanda Nogueira-Pedro, Edson Makiyama, Célia Colli, Ricardo Ambrósio Fock
Magnesium (Mg2+ ) is a mineral with the ability to influence cell proliferation and to modulate inflammatory/immune responses, due to its anti-inflammatory properties. In addition, mesenchymal stem cells (MSCs) modulate the function of all major immune cell populations. Knowing that, the current work aimed to investigate the effects of Mg2+ enrichment, and its influence on the immunomodulatory capacity of MSCs. Murine C3H/10T1/2 MSCs were cultivated in media with different concentrations of Mg2+ (0, 1, 3 and 5 mM), in order to evaluate the effects of Mg2+ on MSC immunomodulatory properties, cell proliferation rates, expression of NFκB and STAT-3, production of IL-1β, IL-6, TGF-β, IL-10, PGE2 and NO, and TRPM7 expression...
May 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29520722/human-umbilical-cord-matrix-stem-cells-reverse-oxidative-stress-induced-cell-death-and-ameliorate-motor-function-and-striatal-atrophy-in-rat-model-of-huntington-disease
#17
Mohammad Javad Ebrahimi, Abbas Aliaghaei, Mahdi Eskandarian Boroujeni, Fariba Khodagholi, Gholamhoussein Meftahi, Mohammad Amin Abdollahifar, Houssein Ahmadi, Samira Danyali, Mahtab Daftari, Yousef Sadeghi
Huntington disease (HD) is an inherited disorder hallmarked by progressive deterioration of specific neurons, followed by movement and cognitive anomalies. Cell therapy approaches in neurodegenerative conditions have concentrated on the replenishment of lost/dying neurons with functional ones. Multipotent mesenchymal stem cells (MSCs) have been represented as a potential remedy for HD. In this study, we evaluated the in vitro and in vivo efficacy of umbilical cord matrix stem cells (UCMSCs) and their paracrine effect against oxidative stress with a specific focus on HD...
March 8, 2018: Neurotoxicity Research
https://www.readbyqxmd.com/read/29518183/cardioprotection-by-cardiac-progenitor-cell-secreted-exosomes-role-of-pregnancy-associated-plasma-protein-a
#18
Lucio Barile, Elisabetta Cervio, Vincenzo Lionetti, Giuseppina Milano, Alessandra Ciullo, Vanessa Biemmi, Sara Bolis, Claudia Altomare, Marco Matteucci, Dario Disilvestre, Tudor Emanuel Fertig, Tiziano Torre, Stefanos Demertzis, Pierluigi Mauri, Tiziano Moccetti, Giuseppe Vassalli
Aims: Cell therapy trials using cardiac-resident progenitor cells (CPCs) and bone marrow-derived mesenchymal stem/progenitor cells (BMCs) in patients after myocardial infarction have provided encouraging results. Exosomes, nanosized extracellular vesicles of endosomal origin, figure prominently in the bioactivities of these cells. However, a head-to-head comparison of exosomes from the two cell types has not been performed yet. Methods and Results: CPCs and BMCs were derived from cardiac atrial appendage specimens and sternal bone marrow, respectively, from patients (n=20; age, 69...
March 5, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29516916/the-effect-of-mesenchymal-stem-cell-conditioned-medium-on-proliferation-and-apoptosis-of-breast-cancer-cell-line
#19
Leila Farahmand, Rezvan Esmaeili, Leila Eini, Keivan Majidzadeh-A
Purpose: Bone marrow-derived mesenchymal stem cells (MSCs) have the potential ability to differentiate into bone, muscle, fat, and cartilage lineage cells. Furthermore, MSCs are known to migrate into tumor-associated stroma of cancer. This tumor microenvironment consists of a dynamic network of growth factors, immune cells, fibroblasts, extracellular matrix, and MSCs. MSCs as nonhematopoietic stem cells affect tumor, epithelial cells by alteration proliferative capacity, morphology, and aggregation pattern of tumor cells...
January 2018: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/29506631/the-osmolyte-type-affects-cartilage-associated-pathologic-marker-expression-during-in-vitro-mesenchymal-stem-cell-chondrogenesis-under-hypertonic-conditions
#20
Sorour Ahmadyan, Mahboubeh Kabiri, Noushin Tasharofi, Simzar Hosseinzadeh, Mousa Kehtari, Athena Hajari Zadeh, Masoud Soleimani, Ali Farazmand, Hana Hanaee-Ahvaz
Stem cells' fate during in vitro differentiation is influenced by biophysicochemical cues. Osmotic stress has proved to enhance chondrocyte marker expression, however its potent negative impacts had never been surveyed. We questioned whether specific osmotic conditions, regarding the osmolyte agent, could benefit chondrogenesis while dampening undesired concomitant hypertrophy and inflammatory responses. To examine the potential side effects of hypertonicity, we assessed cell proliferation as well as chondrogenic and hypertrophic marker expression of human Adipose Derived-MSC after a two week induction in chondrogenic media with either NaCl or Sorbitol, as the osmolyte agent to reach a +100 mOsm hypertonic condition...
February 28, 2018: Cellular and Molecular Biology
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