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Casein kinase 2

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https://www.readbyqxmd.com/read/29666854/casein-glycomacropeptide-hydrolysates-inhibit-pge2-production-and-cox2-expression-in-lps-stimulated-raw-264-7-macrophage-cells-via-akt-mediated-nf-%C3%AE%C2%BAb-and-mapk-pathways
#1
Tiange Li, Dongxiao Gao, Min Du, Xue Cheng, Xueying Mao
A casein glycomacropeptide hydrolysate (GMPH) was found to possess inhibitory activity against lipopolysaccharide (LPS)-induced inflammatory response in our previous study. In the current study, the inhibitory effect and the underlying molecular mechanism of GMPH on inflammatory response in LPS-stimulated RAW264.7 macrophages were further investigated. Results showed that GMPH significantly suppressed LPS-induced intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) production. GMPH reduced the production of prostaglandin E2 (PEG2) and the expression of cyclooxygenase-2 (COX-2) and cytosolic phospholipase A2 (cPLA2) in LPS-stimulated macrophages...
April 18, 2018: Food & Function
https://www.readbyqxmd.com/read/29658601/silencing-of-casein-kinase-2-inhibits-pkc%C3%A2-induced-cell-invasion-by-targeting-mmp%C3%A2-9-in-mcf%C3%A2-7-cells
#2
Jeong-Mi Kim, Eun-Mi Noh, Hyun-Kyung Song, Yong-Ouk You, Sung Hoo Jung, Jong-Suk Kim, Kang-Beom Kwon, Young-Rae Lee, Hyun Jo Youn
Casein kinase 2 (CK2) is a serine/threonine protein kinase that has been considered to represent an important factor in mammary tumorigenesis. Increased expression of matrix metalloproteinase‑9 (MMP‑9) via nuclear factor‑κB (NF‑κB) activation has been demonstrated to promote breast cancer cell invasion. In the present study, the involvement of CK2 in protein kinase C (PKC) induced cell invasion in MCF‑7 breast cancer cells was investigated as well as the underlying molecular mechanisms. The mRNA and protein levels of MMP‑9 in MCF‑7 cells were investigated using reverse transcription‑quantitative polymerase chain reaction, western blot analyses and a zymography assay...
April 13, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29620268/induction-of-huh%C3%A2-7-cell-apoptosis-by-hcv-core-proteins-via-ck1%C3%AE-%C3%A2-p53%C3%A2-bid-signaling-pathway
#3
Shanshan Shen, Chunyang Li, Mingjia Dai, Xuebing Yan
Hepatitis C virus (HCV)‑infected liver cells sensitize host cells to tumor necrosis factor (TNF)‑related apoptosis‑inducing ligand (TRAIL)‑induced cell apoptosis; however, the precise mechanisms are unknown. In the present study, flow cytometry demonstrated that the Annexin V‑positive Huh‑7 cell number was higher in groups transfected with core proteins when compared with the pcDNA3.1 group. The mRNA and protein expression levels of B‑cell lymphoma 2 (Bcl‑2) were negatively associated, while Bcl‑2‑associated X protein (Bax) were positively correlated, with cell apoptotic rate, which, were verified by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blotting...
April 5, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29581866/calpastatin-phosphorylation-regulates-radiation-induced-calpain-activity-in-glioblastoma
#4
Emily A Bassett, Kamalakannan Palanichamy, Mitchell Pearson, Joseph P McElroy, Saikh Jaharul Haque, Erica Hlavin Bell, Arnab Chakravarti
Glioblastoma (GBM) is an aggressive, malignant brain tumor that inevitably develops resistance to conventional chemotherapy and radiation treatments. In order to identify signaling pathways involved in the development of radiation resistance, we performed mass spectrometry-based phospho-proteomic profiling of GBM cell lines and normal human astrocytes before and after radiation treatment. We found radiation induced phosphorylation of a number of proteins including calpastatin, specifically in GBM stem cells (GSCs)...
March 6, 2018: Oncotarget
https://www.readbyqxmd.com/read/29569955/placental-growth-factor-reverses-decreased-vascular-and-uteroplacental-mmp-2-and-9-and-increased-mmp-1-and-7-and-collagen-i-and-iv-in-hypertensive-pregnancy
#5
Zongli Ren, Ning Cui, Minglin Zhu, Raouf A Khalil
Preeclampsia is a complication of pregnancy manifested as maternal hypertension (HTN-Preg) and fetal growth restriction (IUGR), with unclear mechanism. Placental ischemia increases antiangiogenic soluble fms-like tyrosine-kinase-1 (sFlt-1) relative to angiogenic placental-growth-factor (PlGF); however, the molecular targets are unclear. To test the hypothesis that placental ischemia-induced changes in sFlt-1 and PlGF target vascular and uteroplacental matrix metalloproteinases (MMPs), we tested if raising sFlt-1/PlGF ratio by infusing sFlt-1 (10 µg/kg/day) in pregnant (Preg) rats increases blood pressure (BP) and alters MMPs, and if correcting sFlt-1/PlGF by infusing PlGF (20 µg/kg/day) in Preg rats with reduced uterine perfusion pressure (RUPP) improves BP and reverses the changes in MMPs...
March 23, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29556379/ecto-protein-kinase-ck2-the-neglected-form-of-ck2
#6
REVIEW
Mathias Montenarh, Claudia Götz
Ecto-protein kinases, including protein kinase CK2 (former name, casein kinase 2), have been the focus of research for more than 30 years. At the beginning of the ecto-kinase research their identification was performed with substrates and inhibitors whose specificity under the current knowledge was rather limited. Since all currently known ecto-kinases, including ecto-CK2, have intracellular counterparts, one has to exclude that an ecto-localization originates from intracellular counterparts after cell damage...
April 2018: Biomedical Reports
https://www.readbyqxmd.com/read/29547721/the-dietary-supplement-chondroitin-4-sulfate-exhibits-oncogene-specific-pro-tumor-effects-on-braf-v600e-melanoma-cells
#7
Ruiting Lin, Siyuan Xia, Changliang Shan, Dong Chen, Yijie Liu, Xue Gao, Mei Wang, Hee-Bum Kang, Yaozhu Pan, Shuangping Liu, Young Rock Chung, Omar Abdel-Wahab, Taha Merghoub, Michael Rossi, Ragini R Kudchadkar, David H Lawson, Fadlo R Khuri, Sagar Lonial, Jing Chen
Dietary supplements such as vitamins and minerals are widely used in the hope of improving health but may have unidentified risks and side effects. In particular, a pathogenic link between dietary supplements and specific oncogenes remains unknown. Here we report that chondroitin-4-sulfate (CHSA), a natural glycosaminoglycan approved as a dietary supplement used for osteoarthritis, selectively promotes the tumor growth potential of BRAF V600E-expressing human melanoma cells in patient- and cell line-derived xenograft mice and confers resistance to BRAF inhibitors...
March 15, 2018: Molecular Cell
https://www.readbyqxmd.com/read/29537926/changes-in-potassium-channel-modulation-may-underlie-afterhyperpolarization-plasticity-in-oxytocin-neurons-during-late-pregnancy
#8
Lie Wang, Giri Kumar Chandaka, Robert C Foehring, Joseph C Callaway, William E Armstrong
Oxytocin (OT) neurons exhibit larger afterhyperpolarizations (AHPs) following spike trains during pregnancy and lactation, when these neurons burst and release more OT. Calcium-dependent AHPs mediated by SK channels show this plasticity, and are reduced when the channel is phosphorylated by casein kinase 2 (CK2), and increased when dephosphorylated by protein phosphatase 2A (PP2A), by altering Ca2+ sensitivity. We compared AHP currents in supraoptic OT neurons after CK2 inhibition with 4,5,6,7-tetrabromobenzotriazole (TBB), or PP1-PP2A inhibition with okadaic acid (OA) focusing on the peak current at 100 ms representing the SK-mediated, medium AHP (ImAHP )...
March 14, 2018: Journal of Neurophysiology
https://www.readbyqxmd.com/read/29536773/1-benzo-d-thiazol-2-yl-3-phenylureas-as-dual-inhibitors-of-casein-kinase-1-and-abad-enzymes-for-treatment-of-neurodegenerative-disorders
#9
Ondrej Benek, Lukas Hroch, Laura Aitken, Frank Gunn-Moore, Lucie Vinklarova, Kamil Kuca, Daniel I Perez, Concepcion Perez, Ana Martinez, Zdenek Fisar, Kamil Musilek
Several neurodegenerative disorders including Alzheimer's disease (AD) have been connected with deregulation of casein kinase 1 (CK1) activity. Inhibition of CK1 therefore presents a potential therapeutic strategy against such pathologies. Recently, novel class of CK1-specific inhibitors with N-(benzo[d]thiazol-2-yl)-2-phenylacetamide structural scaffold has been discovered. 1-(benzo[d]thiazol-2-yl)-3-phenylureas, on the other hand, are known inhibitors amyloid-beta binding alcohol dehydrogenase (ABAD), an enzyme also involved in pathophysiology of AD...
December 2018: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/29494643/the-importance-of-negative-determinants-as-modulators-of-ck2-targeting-the-lesson-of-akt2-s131
#10
Jordi Vilardell, Cristina Girardi, Oriano Marin, Giorgio Cozza, Lorenzo A Pinna, Maria Ruzzene
CK2 is a pleiotropic S/T protein kinase (formerly known as casein kinase 2) which is attracting increasing interest as therapeutic target, and the identification of its substrates is a crucial step in determining its involvement in different pathological conditions. We recently found that S131 of Akt2 (homologous to the well established CK2 target S129 of Akt1) is not phosphorylated by CK2 either in vitro or in vivo, although the consensus sequence recognized by CK2 (S/T-x-x-E/D/pS/pT) is conserved in it. Here, by exploiting synthetic peptides, in cell transfection experiments, and computational analysis, we show that a single sequence element, a T at position n+1, hampers phosphorylation, causing an α-helix structure organization which prevents the recognition of its own consensus by CK2...
2018: PloS One
https://www.readbyqxmd.com/read/29479054/application-of-high-throughput-assays-to-examine-phospho-modulation-of-the-late-steps-of-regulated-exocytosis
#11
Prabhodh S Abbineni, Jens R Coorssen
Abstract : Regulated exocytosis enables a range of physiological functions including neurotransmission, and the late steps (i.e., docking, priming and Ca2+ -triggered membrane fusion) are modulated by a highly conserved set of proteins and lipids. Many of the molecular components and biochemical interactions required have been identified; the precise mechanistic steps they modulate and the biochemical interactions that need to occur across steps are still the subject of intense investigation. Particularly, although the involvement of phosphorylation in modulating exocytosis has been intensively investigated over the past three decades, it is unclear which phosphorylation events are a conserved part of the fundamental fusion mechanism and/or serve as part of the physiological fusion machine (e...
November 13, 2017: High-throughput
https://www.readbyqxmd.com/read/29475723/umbralisib-a-novel-pi3k%C3%AE-and-casein-kinase-1%C3%AE%C2%B5-inhibitor-in-relapsed-or-refractory-chronic-lymphocytic-leukaemia-and-lymphoma-an-open-label-phase-1-dose-escalation-first-in-human-study
#12
Howard A Burris, Ian W Flinn, Manish R Patel, Timothy S Fenske, Changchun Deng, Danielle M Brander, Martin Gutierrez, James H Essell, John G Kuhn, Hari P Miskin, Peter Sportelli, Michael S Weiss, Swaroop Vakkalanka, Michael R Savona, Owen A O'Connor
BACKGROUND: Umbralisib (TGR-1202) is a novel next-generation inhibitor of phosphatidylinositol 3-kinase (PI3K) isoform p110δ (PI3Kδ), which is structurally distinct from other PI3Kδ inhibitors and shows improved isoform selectivity. Umbralisib also uniquely inhibits casein kinase-1ε, a major regulator of protein translation. The aim of this first-in-human phase 1 study was to establish the safety and preliminary activity profile of umbralisib in patients with haematological malignancies...
February 20, 2018: Lancet Oncology
https://www.readbyqxmd.com/read/29463694/desynchrony-between-brain-and-peripheral-clocks-caused-by-ck1%C3%AE-%C3%AE%C2%B5-disruption-in-gaba-neurons-does-not-lead-to-adverse-metabolic-outcomes
#13
Vincent van der Vinne, Steven J Swoap, Thomas J Vajtay, David R Weaver
Circadian disruption as a result of shift work is associated with adverse metabolic consequences. Internal desynchrony between the phase of the suprachiasmatic nuclei (SCN) and peripheral clocks is widely believed to be a major factor contributing to these adverse consequences, but this hypothesis has never been tested directly. A GABAergic Cre driver combined with conditional casein kinase mutations ( Vgat-Cre + CK1δ fl/fl ε fl/+ ) was used to lengthen the endogenous circadian period in GABAergic neurons, including the SCN, but not in peripheral tissues, to create a Discordant mouse model...
February 20, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29455507/-effect-and-mechanism-of-total-flavone-of-epimedium-on-primary-callus-formation-in-ovariectomized-rats-with-fractures
#14
Xiao-Lin Shi, Bo-Cheng Liang, Peng Wu, Bo Wang, Zhen-Yu Shi, Ling-Cheng Kong, Jian-Liang Yao, Jun-Jun Huang, Chun-Wen Li
OBJECTIVE: To explore the effects and related mechanisms of total flavone of epimedium treatment(TFE)on primary callus for mation in ovariectomized rats. METHODS: Forty male SD rats weighted from 209 to 246 g and aged 6 to 8 weeks were selected. Six weeks after ovariectomy a femur fracture model with middiaphyseal segment fracture was established, estimated and randomly divided into TFE group (150 mg·kg⁻¹·d⁻¹) and control group(received saline). HE staining was used to evaluate the morphologic difference of primary callus during the bone callus healing between these two groups...
August 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29448105/casein-kinase-2-phosphorylates-and-stabilizes-c-ebp%C3%AE-in-pancreatic-%C3%AE-cells
#15
Tomoko Takai, Tomokazu Matsuda, Yuki Matsuura, Kaho Inoue, Emi Suzuki, Ayumi Kanno, Maki Kimura-Koyanagi, Shun-Ichiro Asahara, Naoya Hatano, Wataru Ogawa, Yoshiaki Kido
During the development of type 2 diabetes, endoplasmic reticulum (ER) stress leads to pancreatic β cell failure. CCAAT/enhancer-binding protein (C/EBP) β is highly induced by ER stress and AMP-activated protein kinase (AMPK) suppression in pancreatic β cells, and its accumulation reduces pancreatic β cell mass. We investigated the phosphorylation state of C/EBPβ under these conditions. Casein kinase 2 (CK2) was found to co-localize with C/EBPβ in MIN6 cells. It phosphorylated S222 of C/EBPβ, a previously unidentified phosphorylation site...
February 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29435722/l-arginine-regulates-protein-turnover-in-porcine-mammary-epithelial-cells-to-enhance-milk-protein-synthesis
#16
Qingquan Ma, Shengdi Hu, Makoto Bannai, Guoyao Wu
Milk is an important food for mammalian neonates, but its insufficient production is a nutritional problem for humans and other animals. Recent studies indicate that dietary supplementation with L-arginine (Arg) increases milk production in mammals, including sows, rabbits, and cows. However, the underlying molecular mechanisms remain largely unknown. The present study was conducted with porcine mammary epithelial cells (PMECs) to test the hypothesis that Arg enhances milk protein synthesis via activation of the mechanistic target of rapamycin (mTOR) cell signaling...
February 12, 2018: Amino Acids
https://www.readbyqxmd.com/read/29422872/myocardial-ckip-1-overexpression-protects-from-simulated-microgravity-induced-cardiac-remodeling
#17
Shukuan Ling, Yuheng Li, Guohui Zhong, Yongjun Zheng, Qing Xu, Dingsheng Zhao, Weijia Sun, Xiaoyan Jin, Hongxing Li, Jianwei Li, Huiyuan Sun, Dengchao Cao, Jinping Song, Caizhi Liu, Xinxin Yuan, Xiaorui Wu, Yinlong Zhao, Zizhong Liu, Qi Li, Yingxian Li
Human cardiovascular system has adapted to Earth's gravity of 1G. The microgravity during space flight can induce cardiac remodeling and decline of cardiac function. At present, the mechanism of cardiac remodeling induced by microgravity remains to be disclosed. Casein kinase-2 interacting protein-1 (CKIP-1) is an important inhibitor of pressure-overload induced cardiac remodeling by decreasing the phosphorylation level of HDAC4. However, the role of CKIP-1 in the cardiac remodeling induced by microgravity is unknown...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29384474/targeting-posttranslational-modifications-of-riok1-inhibits-the-progression-of-colorectal-and-gastric-cancers
#18
Xuehui Hong, He Huang, Xingfeng Qiu, Zhijie Ding, Xing Feng, Yuekun Zhu, Huiqin Zhuo, Jingjing Hou, Jiabao Zhao, Wangyu Cai, Ruihua Sha, Xinya Hong, Yongxiang Li, Hongjiang Song, Zhiyong Zhang
RIOK1 has recently been shown to play important roles in cancers, but its posttranslational regulation is largely unknown. Here we report that RIOK1 is methylated at K411 by SETD7 methyltransferase and that lysine-specific demethylase 1 (LSD1) reverses its methylation. The mutated RIOK1 (K411R) that cannot be methylated exhibits a longer half-life than does the methylated RIOK1. FBXO6 specifically interacts with K411-methylated RIOK1 through its FBA domain to induce RIOK1 ubiquitination. Casein kinase 2 (CK2) phosphorylates RIOK1 at T410, which stabilizes RIOK1 by antagonizing K411 methylation and impeding the recruitment of FBXO6 to RIOK1...
January 31, 2018: ELife
https://www.readbyqxmd.com/read/29382046/how-do-we-study-the-dynamic-structure-of-unstructured-proteins-a-case-study-on-nopp140-as-an-example-of-a-large-intrinsically-disordered-protein
#19
REVIEW
Jung-Hyun Na, Won-Kyu Lee, Yeon Gyu Yu
Intrinsically disordered proteins (IDPs) represent approximately 30% of the human genome and play key roles in cell proliferation and cellular signaling by modulating the function of target proteins via protein-protein interactions. In addition, IDPs are involved in various human disorders, such as cancer, neurodegenerative diseases, and amyloidosis. To understand the underlying molecular mechanism of IDPs, it is important to study their structural features during their interactions with target proteins. However, conventional biochemical and biophysical methods for analyzing proteins, such as X-ray crystallography, have difficulty in characterizing the features of IDPs because they lack an ordered three-dimensional structure...
January 27, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29374533/circadian-control-of-p75-neurotrophin-receptor-leads-to-alternate-activation-of-nrf2-and-c-rel-to-reset-energy-metabolism-in-astrocytes-via-brain-derived-neurotrophic-factor
#20
REVIEW
Tetsuro Ishii, Eiji Warabi, Giovanni E Mann
Circadian clock genes regulate energy metabolism partly through neurotrophins in the body. The low affinity neurotrophin receptor p75NTR is a clock component directly regulated by the transcriptional factor Clock:Bmal1 complex. Brain-derived neurotrophic factor (BDNF) is expressed in the brain and plays a key role in coordinating metabolic interactions between neurons and astrocytes. BDNF transduces signals through TrkB and p75NTR receptors. This review highlights a novel molecular mechanism by which BDNF via circadian control of p75NTR leads to daily resetting of glucose and glycogen metabolism in brain astrocytes to accommodate their functional interaction with neurons...
January 24, 2018: Free Radical Biology & Medicine
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