keyword
MENU ▼
Read by QxMD icon Read
search

Xpert MTB

keyword
https://www.readbyqxmd.com/read/29791546/rapid-molecular-test-for-tuberculosis-impact-of-its-routine-use-at-a-referral-hospital
#1
Marilda Casela, Silvânia Maria Andrade Cerqueira, Thais de Oliveira Casela, Mariana Araújo Pereira, Samanta Queiroz Dos Santos, Franco Andres Del Pozo, Songeli Menezes Freire, Eliana Dias Matos
OBJECTIVE: To evaluate the impact of the use of the molecular test for Mycobacterium tuberculosis and its resistance to rifampin (Xpert MTB/RIF), under routine conditions, at a referral hospital in the Brazilian state of Bahia. METHODS: This was a descriptive study using the database of the Mycobacteriology Laboratory of the Octávio Mangabeira Specialized Hospital, in the city of Salvador, and georeferencing software. We evaluated 3,877 sputum samples collected from symptomatic respiratory patients, under routine conditions, between June of 2014 and March of 2015...
April 2018: Jornal Brasileiro de Pneumologia: Publicaça̋o Oficial da Sociedade Brasileira de Pneumologia e Tisilogia
https://www.readbyqxmd.com/read/29791449/impact-of-involvement-of-non-formal-health-providers-on-tb-case-notification-among-migrant-slum-dwelling-populations-in-odisha-india
#2
Ambarish Dutta, Sarthak Pattanaik, Rajendra Choudhury, Pritish Nanda, Suvanand Sahu, Rajendra Panigrahi, Bijaya K Padhi, Krushna Chandra Sahoo, P R Mishra, Pinaki Panigrahi, Daisy Lekharu, Robert H Stevens
BACKGROUND: Migrant labourers living in the slums of urban and industrial patches across India make up a key sub-population so far controlling Tuberculosis (TB) in the country is concerned. This is because many TB patients from these communities- remain under reached by the Revised National Tuberculosis Control Programme (RNTCP) of India. This marginalized community usually seeks early-stage healthcare from "friendly neighbourhood" non-formal health providers (NFHPs). Because, RNTCP has limited capacity to involve the NFHPs, an implementation research project was conceived, whereby an external partner would engage with the NFHPs to enable them to identify early TB symptomatics from this key sub-population who would be then tested using Xpert MTB/RIF technology...
2018: PloS One
https://www.readbyqxmd.com/read/29785129/performance-of-the-abbott-realtime-mtb-rif-inh-resistance-assay-when-used-to-test-mycobacterium-tuberculosis-specimens-from-bangladesh
#3
Joshua Kostera, Gregor Leckie, Klara Abravaya, Hong Wang
Introduction: The Abbott RealTime MTB RIF/INH Resistance Assay (RT MTB RIF/INH) is an assay for the detection of rifampicin (RIF)- and/or isoniazid (INH)-resistant Mycobacterium tuberculosis (MTB). The assay can be used to test sputum, bronchial alveolar lavage, and N-Acetyl-L-Cysteine (NALC)/NaOH pellets prepared from these samples. The assay can be used in direct testing mode, or in reflex mode following a MTB positive result produced by its companion assay, Abbott RT MTB. Methods: In this study, the direct testing mode was used to test paired sputum and NALC/NaOH pellets prepared from sputum collected from Bangladesh TB patients...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29779775/accuracy-of-whole-genome-sequencing-versus-phenotypic-mgit-and-commercial-molecular-tests-for-detection-of-drug-resistant-mycobacterium-tuberculosis-isolated-from-patients-in-brazil-and-mozambique
#4
Cinara Silva Feliciano, Evangelina Inacio Namburete, Jéssica Rodrigues Plaça, Kamila Peronni, Anzaan Dippenaar, Robin Mark Warren, Wilson Araújo Silva, Valdes Roberto Bollela
BACKGROUND: The fast and accurate diagnosis of drug-resistant tuberculosis (DR-TB) is critical to reducing the spread of disease. Although commercial genotypic drug-susceptibility tests (DST) are close to the goal, they are still not able to detect all relevant DR-TB related mutations. Whole genome sequencing (WGS) allows better comprehension of DR-TB with a great discriminatory power. We aimed to evaluate WGS in M. tuberculosis isolates compared with phenotypic and genotypic DST. METHODS: This cross-sectional study evaluated 30 isolates from patients with detected DR-TB in Brazil and Mozambique...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779774/the-impact-of-repeated-nalc-naoh-decontamination-on-the-performance-of-xpert-mtb-rif-assay
#5
Andrea Rachow, Elmar Saathoff, Bariki Mtafya, Daniel Mapamba, Chacha Mangu, Gabriel Rojas-Ponce, Nyanda E Ntinginya, Martin Boeree, Norbert Heinrich, Stephen H Gillespie, Michael Hoelscher
The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin ). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29770076/the-use-of-technology-to-improve-health-care-to-saskatchewan-s-first-nations-communities
#6
I Khan, N Ndubuka, K Stewart, V McKinney, I Mendez
Background: Saskatchewan is a province of over one million people and over 13% are Indigenous peoples, many of whom live on reserve lands. Despite continued efforts, access to health care remains a significant challenge for these Indigenous people, especially those in the North. Objective: To address this challenge, Saskatchewan's health care providers have been incorporating the use of technology for various health services. This paper describes various ways technology has been used in First Nations communities in Saskatchewan...
June 1, 2017: Canada Communicable Disease Report, Relevé des Maladies Transmissibles Au Canada
https://www.readbyqxmd.com/read/29768561/the-role-of-the-xpert-mtb-rif-assay-among-adolescents-suspected-of-pulmonary-tuberculosis-in-rio-de-janeiro-brazil
#7
Thania Luiza de Andrade Sieiro, Rafaela Baroni Aurílio, Elizabeth Cristina C Soares, Silvia S Chiang, Clemax Couto Sant Anna
INTRODUCTION: The teste rápido molecular para tuberculose (TRM-TB) was introduced in 2014 in Brazil for tuberculosis screening. However, its role in adolescents in Brazil has not been studied. METHODS: A descriptive study of adolescents with suspected tuberculosis using National Laboratory software. RESULTS: Of 852 (15.4%) suspected cases, 131 were positive by TRM-TB and 2% were resistant to rifampicin. Among TRM-TB-positive cases, 105 (91...
March 2018: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29751798/clinic-flow-for-sti-hiv-and-tb-patients-in-an-urban-infectious-disease-clinic-offering-point-of-care-testing-services-in-durban-south-africa
#8
Katrina J Stime, Nigel Garrett, Yukteshwar Sookrajh, Jienchi Dorward, Ntuthu Dlamini, Ayo Olowolagba, Monisha Sharma, Ruanne V Barnabas, Paul K Drain
BACKGROUND: Many clinics in Southern Africa have long waiting times. The implementation of point-of-care (POC) tests to accelerate diagnosis and improve clinical management in resource-limited settings may improve or worsen clinic flow and waiting times. The objective of this study was to describe clinic flow with special emphasis on the impact of POC testing at a large urban public healthcare clinic in Durban, South Africa. METHODS: We used time and motion methods to directly observe patients and practitioners...
May 11, 2018: BMC Health Services Research
https://www.readbyqxmd.com/read/29740714/xpert-mtb-rif-diagnosis-of-childhood-tuberculosis-from-sputum-and-stool-samples-in-a-high-tb-hiv-prevalent-setting
#9
Patrick Orikiriza, Margaret Nansumba, Dan Nyehangane, Mathieu Bastard, Ivan Taremwa Mugisha, Denis Nansera, Juliet Mwanga-Amumpaire, Yap Boum, Elias Kumbakumba, Maryline Bonnet
The Xpert MTB/RIF assay is a major advance for diagnosis of tuberculosis (TB) in high-burden countries but is limited in children by their difficulty to produce sputum. We investigated TB in sputum and stool from children with the aim of improving paediatric TB diagnosis. A prospective cohort of children with presumptive TB, provided two sputum or induced sputum at enrolment in a regional referral hospital in Uganda. Stool was collected from those started on TB treatment. All specimen were tested for Xpert MTB/RIF, mycobacteria growth indicator tube (MGIT), Lowenstein Jensen cultures and microscopy (except stool)...
May 8, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29707989/diagnostic-usefulness-of-bronchoalveolar-lavage-fluid-xpert-mtb-rif-in-pauci-bacillary-pulmonary-tuberculosis
#10
Jeongmin Hong, Seung Hyun Lee, Byung-Han Ryu, Min Jae Kim, Kyung-Wook Jo, Yong Pil Chong, Sang-Oh Lee, Sang-Ho Choi, Tae Sun Shim, Yang Soo Kim, Jun Hee Woo, Sung-Han Kim
No abstract text is available yet for this article.
April 28, 2018: Infectious Diseases
https://www.readbyqxmd.com/read/29707322/incremental-cost-effectiveness-of-the-second-xpert-mtb-rif-assay-to-detect-mycobacterium-tuberculosis
#11
Guirong Wang, Shuqi Wang, Guanglu Jiang, Yuhong Fu, Yuanyuan Shang, Hairong Huang
Background: Due to the non-homogeneity of specimens collected from tuberculosis (TB) suspects, repeated Xpert MTB/RIF (Xpert) may have potential clinical benefits. Incremental cost-effectiveness was analyzed for the second Xpert assay to detect Mycobacterium tuberculosis (Mtb) and rifampicin (RIF) resistance. Methods: Specimens were collected from 1,063 pulmonary TB (PTB) and 398 extrapulmonary TB (EPTB) suspects, who had two Xpert tests sequentially within one week...
March 2018: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29692304/diagnostic-and-treatment-monitoring-potential-of-a-stool-based-quantitative-polymerase-chain-reaction-assay-for-pulmonary-tuberculosis
#12
Andrew R DiNardo, Alexander W Kay, Gugu Maphalala, Nadine M Harris, Celia Fung, Godwin Mtetwa, Pilar Ustero, Sindisiwe Dlamini, Ngan Ha, Edward A Graviss, Rojelio Mejia, Anna M Mandalakas
A quantifiable, stool-based, Mycobacterium tuberculosis ( Mtb ) test has potential complementary value to respiratory specimens. Limit of detection (LOD) was determined by spiking control stool. Clinical test performance was evaluated in a cohort with pulmonary tuberculosis (TB) ( N = 166) and asymptomatic household TB child contacts ( N = 105). Stool-quantifiable polymerase chain reaction (qPCR) results were compared with sputum acid-fast bacilli (AFB) microscopy, GeneXpert MTB/RIF (Xpert MTB/RIF), and cultures...
April 23, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29663957/high-utility-of-active-tuberculosis-case-finding-in-an-ethiopian-prison
#13
Y Merid, Y Woldeamanuel, M Abebe, D G Datiko, T Hailu, G Habtamu, G Assefa, R R Kempker, H M Blumberg, A Aseffa
SETTING: Hawassa Prison, Southern Region of Ethiopia. OBJECTIVE: To determine the burden of pulmonary tuberculosis (TB) using active case finding among prisoners. DESIGN: In this cross-sectional study, prisoners were screened for TB using a symptom screen. Those with cough of 2 weeks had spot and morning sputum samples collected for acid-fast bacilli (AFB) smear microscopy and molecular diagnostic testing (Xpert® MTB/RIF). RESULTS: Among 2068 prisoners, 372 (18%) had a positive cough screen...
May 1, 2018: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/29593688/a-comprehensive-evaluation-of-xpert-mtb-rif-assay-with-bronchoalveolar-lavage-fluid-as-a-single-test-or-combined-with-conventional-assays-for-diagnosis-of-pulmonary-tuberculosis-in-china-a-two-center-prospective-study
#14
Xiaofu Pan, Shoufeng Yang, Margaret A Deighton, Yue Qu, Liang Hong, Feifei Su
Introduction: The Xpert MTB/RIF is recommended by the World Health Organization as a first line rapid test for the diagnosis of pulmonary tuberculosis (TB); however, China does not routinely use this test, partially due to the lack of a sufficient number of systematic evaluations of this assay in local patients. The aims of this study were to comprehensively assess the diagnostic performance of Xpert MTB/RIF, either alone or in combination with conventional assays for the diagnosis of pulmonary TB in adult Chinese patients...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29584793/characteristics-and-management-of-presumptive-tuberculosis-in-public-health-facilities-in-malawi-2014-2016
#15
W Ng'ambi, S Gugsa, H Tweya, B Girma, H Kanyerere, I Dambe, Y Babaye, J Mpunga, S Phiri
Setting: Public health facilities providing tuberculosis (TB) and human immunodeficiency virus (HIV) services in Malawi. Objectives: Using routinely collected health service delivery data to describe trends in HIV ascertainment and use of the Xpert® MTB/RIF assay to diagnose TB among HIV-positive presumptive TB cases. Design: This was an implementation study of presumptive TB cases who sought care from 21 facilities between April 2014 and June 2016. Descriptive statistics were used to summarise patient, facility and service level characteristics...
December 21, 2017: Public Health Action
https://www.readbyqxmd.com/read/29582718/use-of-a-single-xpert-mtb-rif-assay-to-determine-the-duration-of-airborne-isolation-in-hospitalized-patients-with-suspected-pulmonary-tuberculosis
#16
Husain Poonawala, Surbhi Leekha, Sandra Medina-Moreno, Mala Filippell, J Kristie Johnson, Robert R Redfield, Kapil K Saharia
BACKGROUNDHospitalized patients with suspected tuberculosis (TB) are placed in airborne isolation until 3 sputum smear samples are negative for acid-fast bacilli (AFB). The Xpert MTB/RIF assay ("Xpert") nucleic acid amplification test (NAAT) to identify Mycobacterium tuberculosis DNA and resistance to rifampicin is superior to AFB sputum smear microscopy for the diagnosis of TB.OBJECTIVETo compare the performance of a single Xpert to AFB smear microscopy for time to airborne infection isolation (AII) discontinuation...
May 2018: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29581936/unsuccessful-xpert-%C3%A2-mtb-rif-results-the-nigerian-experience
#17
M Gidado, N Nwokoye, P Nwadike, P Ajiboye, R Eneogu, S Useni, J Onazi, A Lawanson, E Elom, A Tubi, J Kuye
Setting: Nigeria, a high tuberculosis (TB) burden country. Objective: To study the rate, distribution and causes of unsuccessful Xpert® MTB/RIF test outcomes, with the aim of identifying key areas that need to be strengthened for optimal performance of the assay. Design: This was a retrospective analysis of data uploaded between January and December 2015 from Xpert facilities to the central server using GXAlert. Result: Of 52 219 test results uploaded from 176 Xpert machines, 22.5% were positive for Mycobacterium tuberculosis, 10...
March 21, 2018: Public Health Action
https://www.readbyqxmd.com/read/29581435/correlation-of-xpert-mtb-rif-with-measures-to-assess-mycobacterium-tuberculosis-bacillary-burden-in-high-hiv-burden-areas-of-southern-africa
#18
Fenella Beynon, Grant Theron, Durval Respeito, Edson Mambuque, Belen Saavedra, Helder Bulo, Sergi Sanz, Keertan Dheda, Alberto L Garcia-Basteiro
Traditionally, smear microscopy has been used as a point-of-care measure of bacillary burden in tuberculosis patients to inform infection control and contact tracing. Xpert MTB/RIF has the potential to replace smear. However, data to support the use of its quantitative output [cycle threshold (CT )] as an alternate point-of-care measure of bacillary burden are limited. This study assessed the correlation (Spearman's) between CT , smear, culture time-to-positivity (TTP), and clinical factors in patients with Xpert-positive sputum from Mozambique (n = 238) and South Africa (n = 462)...
March 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29580818/tuberculosis-advances-and-challenges-in-development-of-new-diagnostics-and-biomarkers
#19
REVIEW
Gerhard Walzl, Ruth McNerney, Nelita du Plessis, Matthew Bates, Timothy D McHugh, Novel N Chegou, Alimuddin Zumla
Tuberculosis remains the leading cause of death from an infectious disease worldwide. Early and accurate diagnosis and detection of drug-sensitive and drug-resistant tuberculosis is essential for achieving global tuberculosis control. Despite the introduction of the Xpert MTB/RIF assay as the first-line rapid tuberculosis diagnostic test, the gap between global estimates of incidence and new case notifications is 4·1 million people. More accurate, rapid, and cost-effective screening tests are needed to improve case detection...
March 23, 2018: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/29566056/accuracy-of-different-xpert-mtb-rif-implementation-strategies-in-programmatic-settings-at-the-regional-referral-hospitals-in-uganda-evidence-for-country-wide-roll-out
#20
Winters Muttamba, Willy Ssengooba, Rogers Sekibira, Bruce Kirenga, Achilles Katamba, Moses Joloba
BACKGROUND: Xpert MTB/RIF assay is a highly sensitive test for TB diagnosis, but still costly to most low-income countries. Several implementation strategies instead of frontline have been suggested; however with scarce data. We assessed accuracy of different Xpert MTB/RIF implementation strategies to inform national roll-out. METHODS: This was a cross-sectional study of 1,924 adult presumptive TB patients in five regional referral hospitals of Uganda. Two sputum samples were collected, one for fluorescent microscopy (FM) and Xpert MTB/RIF examined at the study site laboratories...
2018: PloS One
keyword
keyword
23690
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"