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Nfkb exosome

Lucia Paolini, Andrea Zendrini, Giuseppe Di Noto, Sara Busatto, Elisabetta Lottini, Annalisa Radeghieri, Alessandra Dossi, Andrea Caneschi, Doris Ricotta, Paolo Bergese
Exosomes are gaining a prominent role in research due to their intriguing biology and several therapeutic opportunities. However, their accurate purification from body fluids and detailed physicochemical characterization remain open issues. We isolated exosomes from serum of patients with Multiple Myeloma by four of the most popular purification methods and assessed the presence of residual contaminants in the preparations through an ad hoc combination of biochemical and biophysical techniques - including Western Blot, colloidal nanoplasmonics, atomic force microscopy (AFM) and scanning helium ion microscopy (HIM)...
2016: Scientific Reports
Johnathon D Anderson, Henrik J Johansson, Calvin S Graham, Mattias Vesterlund, Missy T Pham, Charles S Bramlett, Elizabeth N Montgomery, Matt S Mellema, Renee L Bardini, Zelenia Contreras, Madeline Hoon, Gerhard Bauer, Kyle D Fink, Brian Fury, Kyle J Hendrix, Frederic Chedin, Samir El-Andaloussi, Billie Hwang, Michael S Mulligan, Janne Lehtiƶ, Jan A Nolta
Mesenchymal stem cells (MSC) are known to facilitate healing of ischemic tissue related diseases through proangiogenic secretory proteins. Recent studies further show that MSC derived exosomes function as paracrine effectors of angiogenesis, however, the identity of which components of the exosome proteome responsible for this effect remains elusive. To address this we used high-resolution isoelectric focusing coupled liquid chromatography tandem mass spectrometry, an unbiased high throughput proteomics approach to comprehensively characterize the proteinaceous contents of MSCs and MSC derived exosomes...
March 2016: Stem Cells
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