Johnathon D Anderson, Henrik J Johansson, Calvin S Graham, Mattias Vesterlund, Missy T Pham, Charles S Bramlett, Elizabeth N Montgomery, Matt S Mellema, Renee L Bardini, Zelenia Contreras, Madeline Hoon, Gerhard Bauer, Kyle D Fink, Brian Fury, Kyle J Hendrix, Frederic Chedin, Samir El-Andaloussi, Billie Hwang, Michael S Mulligan, Janne Lehtiö, Jan A Nolta
Mesenchymal stem cells (MSC) are known to facilitate healing of ischemic tissue related diseases through proangiogenic secretory proteins. Recent studies further show that MSC derived exosomes function as paracrine effectors of angiogenesis, however, the identity of which components of the exosome proteome responsible for this effect remains elusive. To address this we used high-resolution isoelectric focusing coupled liquid chromatography tandem mass spectrometry, an unbiased high throughput proteomics approach to comprehensively characterize the proteinaceous contents of MSCs and MSC derived exosomes...
March 2016: Stem Cells