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https://www.readbyqxmd.com/read/28822827/host-genes-regulate-transcription-of-sperm-introduced-hepatitis-b-virus-genes-in-embryo
#1
Ying Zhong, Dong-Ling Liu, Mohamed Morsi M Ahmed, Peng-Hao Li, Xiao-Ling Zhou, Qing-Dong Xie, Xiao-Qing Xu, Ting-Ting Han, Zhi-Wei Hou, Chen-Yao Zhong, Ji-Hua Huang, Fei Zeng, Tian-Hua Huang
Hepatitis B virus (HBV) can invade the male germline, and sperm-introduced HBV genes could be transcribed in embryo. This study was to explore whether viral gene transcription is regulated by host genes. Embryos were produced by in vitro fertilization of hamster oocytes with human sperm containing the HBV genome. Total RNA extracted from test and control embryos were subjected to SMART-PCR, SSH, microarray hybridization, sequencing and BLAST analysis. Twenty-nine sequences showing significant identity to five human gene families were identified, with CSH2, EIF4G2, PCBD2, PSG4 and TTN selected to represent target genes...
August 16, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28822801/microarray-gene-expression-study-in-fibroblast-and-lymphoblastoid-cell-lines-from-antipsychotic-na%C3%A3-ve-first-episode-schizophrenia-patients
#2
Patricia Gassó, Sergi Mas, Natalia Rodríguez, Daniel Boloc, Susana García-Cerro, Miquel Bernardo, Amalia Lafuente, Eduard Parellada
Schizophrenia (SZ) is a chronic psychiatric disorder whose onset of symptoms occurs in late adolescence and early adulthood. The etiology is complex and involves important gene-environment interactions. Microarray gene-expression studies on SZ have identified alterations in several biological processes. The heterogeneity in the results can be attributed to the use of different sample types and other important confounding factors including age, illness chronicity and antipsychotic exposure. The aim of the present microarray study was to analyze, for the first time to our knowledge, differences in gene expression profiles in 18 fibroblast (FCLs) and 14 lymphoblastoid cell lines (LCLs) from antipsychotic-naïve first-episode schizophrenia (FES) patients and healthy controls...
August 5, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28822800/amelogenin-induces-m2-macrophage-polarisation-via-pge2-camp-signalling-pathway
#3
Kensuke Yamamichi, Takao Fukuda, Terukazu Sanui, Kyosuke Toyoda, Urara Tanaka, Yuki Nakao, Karen Yotsumoto, Hiroaki Yamato, Takaharu Taketomi, Takeshi Uchiumi, Fusanori Nishimura
OBJECTIVES: Amelogenin, the major component of the enamel matrix derivative (EMD), has been suggested as a bioactive candidate for periodontal regeneration. Apart from producing a regenerative effect on periodontal tissues, amelogenin has also been reported to have an anti-inflammatory effect. However, the precise molecular mechanisms underlying these effects remain unclear. In the present study, we examined the immunomodulatory effects of amelogenin on macrophages. DESIGN: Human phorbol 12-myristate 13-acetate (PMA)-differentiated U937 macrophages and CD14+ peripheral blood-derived monocytes (PBMC)-derived macrophages were stimulated with recombinant amelogenin (rM180)...
August 12, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28822241/transcript-analysis-identifies-differential-uterine-gene-expression-profile-beyond-the-normal-implantation-window-in-mice
#4
Qiliang Xin, Meiling Li, Haibin Wang, Sheng Cui, Guoliang Xia, Shuangbo Kong
Uterine receptivity is defined as a state when the uterine milieu is favorable for blastocyst implantation and it can only last for a limited time period. In this study, by utilizing the embryo transfer model, it was observed that a portion of blastocysts could initiate implantation even when transferred beyond the timing of normal uterine receptivity, while their mid-gestational embryo development exhibited severe retardation, suggesting that the uterine status beyond the normal implantation window is unconducive for normal implantation...
August 5, 2017: Theriogenology
https://www.readbyqxmd.com/read/28822034/microrna-expression-target-genes-and-signaling-pathways-in-infants-with-a-ventricular-septal-defect
#5
Hui Chai, Zhaoyuan Yan, Ke Huang, Yuanqing Jiang, Lin Zhang
This study aimed to systematically investigate the relationship between miRNA expression and the occurrence of ventricular septal defect (VSD), and characterize the miRNA target genes and pathways that can lead to VSD. The miRNAs that were differentially expressed in blood samples from VSD and normal infants were screened and validated by implementing miRNA microarrays and qRT-PCR. The target genes regulated by differentially expressed miRNAs were predicted using three target gene databases. The functions and signaling pathways of the target genes were enriched using the GO database and KEGG database, respectively...
August 18, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28821887/micrornas-218a-5p-219a-5p-and-221-3p-regulate-vestibular-compensation
#6
Mun Young Chang, Sohyeon Park, Jun Jae Choi, Young-Kook Kim, Myung-Whan Suh, Jun Ho Lee, Seung Ha Oh, Moo Kyun Park
Unilateral vestibular deafferentation (UVD) interrupts afferent signals from one side, resulting in an imbalance of the resting activity between bilateral vestibular nuclei. Vestibular compensation is the process of balancing the resting activity to reestablish homeostasis. Here, we investigated microRNAs (miRNAs) that regulate vestibular compensation using the Sprague-Dawley rat. After determining the progression of vestibular compensation following UVD, microarray analysis was performed and nine miRNAs were selected as candidates...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821833/microrna-449c-5p-inhibits-osteogenic-differentiation-of-human-vics-through-smad4-mediated-pathway
#7
Rongjian Xu, Min Zhao, Yun Yang, Zhuo Huang, Chunying Shi, Xianglin Hou, Yannan Zhao, Bing Chen, Zhifeng Xiao, Jianzhou Liu, Qi Miao, Jianwu Dai
Calcific aortic valve disease (CAVD) is the most common heart valve disorder, yet its mechanism remains poorly understood. Valve interstitial cells (VICs) are the prevalent cells in aortic valve and their osteogenic differentiation may be responsible for calcific nodule formation in CAVD pathogenesis. Emerging evidence shows microRNA (miRNA, or miR) can function as important regulators of many pathological processes, including osteogenic differentiation. Here, we aimed to explore the function of miR-449c-5p in CAVD pathogenesis...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821776/mineralization-and-preservation-of-an-extremotolerant-bacterium-isolated-from-an-early-mars-analog-environment
#8
F Gaboyer, C Le Milbeau, M Bohmeier, P Schwendner, P Vannier, K Beblo-Vranesevic, E Rabbow, F Foucher, P Gautret, R Guégan, A Richard, A Sauldubois, P Richmann, A K Perras, C Moissl-Eichinger, C S Cockell, P Rettberg, Marteinsson, E Monaghan, P Ehrenfreund, L Garcia-Descalzo, F Gomez, M Malki, R Amils, P Cabezas, N Walter, F Westall
The artificial mineralization of a polyresistant bacterial strain isolated from an acidic, oligotrophic lake was carried out to better understand microbial (i) early mineralization and (ii) potential for further fossilisation. Mineralization was conducted in mineral matrixes commonly found on Mars and Early-Earth, silica and gypsum, for 6 months. Samples were analyzed using microbiological (survival rates), morphological (electron microscopy), biochemical (GC-MS, Microarray immunoassay, Rock-Eval) and spectroscopic (EDX, FTIR, RAMAN spectroscopy) methods...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821749/dna-methylation-in-demyelinated-multiple-sclerosis-hippocampus
#9
Anthony M Chomyk, Christina Volsko, Ajai Tripathi, Sadie A Deckard, Bruce D Trapp, Robert J Fox, Ranjan Dutta
Multiple Sclerosis (MS) is an immune-mediated demyelinating disease of the human central nervous system (CNS). Memory impairments and hippocampal demyelination are common features in MS patients. Our previous data have shown that demyelination alters neuronal gene expression in the hippocampus. DNA methylation is a common epigenetic modifier of gene expression. In this study, we investigated whether DNA methylation is altered in MS hippocampus following demyelination. Our results show that mRNA levels of DNA methyltransferase were increased in demyelinated MS hippocampus, while de-methylation enzymes were decreased...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821714/gadd45a-plays-a-protective-role-against-temozolomide-treatment-in-glioblastoma-cells
#10
Hsiao-Han Wang, Tsuey-Yu Chang, Wei-Chen Lin, Kuo-Chen Wei, Jyh-Wei Shin
Glioblastoma multiforme (GBM) is one of the most aggressive cancers. Despite recent advances in multimodal therapies, high-grade glioma remains fatal. Temozolomide (TMZ) is an alkylating agent used worldwide for the clinical treatment of GBM; however, the innate and acquired resistance of GBM limits its application. Here, we found that TMZ inhibited the proliferation and induced the G2/M arrest of GBM cells. Therefore, we performed microarrays to identify the cell cycle- and apoptosis-related genes affected by TMZ...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821632/transcriptomic-and-epigenetic-regulation-of-disuse-atrophy-and-the-return-to-activity-in-skeletal-muscle
#11
Andrew G Fisher, Robert A Seaborne, Thomas M Hughes, Alex Gutteridge, Claire Stewart, Judy M Coulson, Adam P Sharples, Jonathan C Jarvis
Physical inactivity and disuse are major contributors to age-related muscle loss. Denervation of skeletal muscle has been previously used as a model with which to investigate muscle atrophy following disuse. Although gene regulatory networks that control skeletal muscle atrophy after denervation have been established, the transcriptome in response to the recovery of muscle after disuse and the associated epigenetic mechanisms that may function to modulate gene expression during skeletal muscle atrophy or recovery have yet to be investigated...
August 17, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28821630/il-17a-deficiency-mitigates-bleomycin-induced-complement-activation-during-lung-fibrosis
#12
Ellyse Cipolla, Amanda J Fisher, Hongmei Gu, Elizabeth A Mickler, Manisha Agarwal, Carol A Wilke, Kevin K Kim, Bethany B Moore, Ragini Vittal
Interleukin 17A (IL-17A) and complement (C') activation have each been implicated in the pathogenesis of idiopathic pulmonary fibrosis (IPF). We have reported that IL-17A induces epithelial injury via TGF-β in murine bronchiolitis obliterans; that TGF-β and the C' cascade present signaling interactions in mediating epithelial injury; and that the blockade of C' receptors mitigates lung fibrosis. In the present study, we investigated the role of IL-17A in regulating C' in lung fibrosis. Microarray analyses of mRNA isolated from primary normal human small airway epithelial cells indicated that IL-17A (100 ng/ml; 24 h; n = 5 donor lungs) induces C' components (C' factor B, C3, and GPCR kinase isoform 5), cytokines (IL8, -6, and -1B), and cytokine ligands (CXCL1, -2, -3, -5, -6, and -16)...
August 17, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28821584/transcriptome-analysis-of-mycobacteria-specific-cd4-t-cells-identified-by-activation-induced-expression-of-cd154
#13
Shajo Kunnath-Velayudhan, Michael F Goldberg, Neeraj K Saini, Christopher T Johndrow, Tony W Ng, Alison J Johnson, Jiayong Xu, John Chan, William R Jacobs, Steven A Porcelli
Analysis of Ag-specific CD4(+) T cells in mycobacterial infections at the transcriptome level is informative but technically challenging. Although several methods exist for identifying Ag-specific T cells, including intracellular cytokine staining, cell surface cytokine-capture assays, and staining with peptide:MHC class II multimers, all of these have significant technical constraints that limit their usefulness. Measurement of activation-induced expression of CD154 has been reported to detect live Ag-specific CD4(+) T cells, but this approach remains underexplored and, to our knowledge, has not previously been applied in mycobacteria-infected animals...
August 18, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28821567/chronic-spinal-cord-changes-in-a-high-fat-diet-fed-male-rat-model-of-thoracic-spinal-contusion
#14
Redin A Spann, William J Lawson, Raymond J Grill, Michael R Garrett, Bernadette E Grayson
Individuals that suffer injury to the spinal cord can result in long-term, debilitating sequelae. Spinal cord injured patients have increased risk for the development of metabolic disease which can further hinder the effectiveness of treatments to rehabilitate the cord and improve quality of life. In the present study, we sought to understand the impact of high-fat diet induced obesity on spinal cord injury (SCI) by examining transcriptome changes in the area of the injury and rostral and caudal to site of damage 12 weeks after injury...
August 18, 2017: Physiological Genomics
https://www.readbyqxmd.com/read/28821562/gtse1-regulates-spindle-microtubule-dynamics-to-control-aurora-b-kinase-and-kif4a-chromokinesin-on-chromosome-arms
#15
Aaron R Tipton, Jonathan D Wren, John R Daum, Joseph C Siefert, Gary J Gorbsky
In mitosis, the dynamic assembly and disassembly of microtubules are critical for normal chromosome movement and segregation. Microtubule turnover varies among different mitotic spindle microtubules, dictated by their spatial distribution within the spindle. How turnover among the various classes of spindle microtubules is differentially regulated and the resulting significance of differential turnover for chromosome movement remains a mystery. As a new tactic, we used global microarray meta-analysis (GAMMA), a bioinformatic method, to identify novel regulators of mitosis, and in this study, we describe G2- and S phase-expressed protein 1 (GTSE1)...
August 18, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28821547/cultivation-in-space-flight-produces-minimal-alterations-in-the-susceptibility-of-bacillus-subtilis-cells-to-72-different-antibiotics-and-growth-inhibiting-compounds
#16
Michael D Morrison, Patricia Fajardo-Cavazos, Wayne L Nicholson
Past results have suggested that bacterial antibiotic susceptibility is altered during space flight. To test this notion, Bacillus subtilis cells were cultivated in matched hardware, medium, and environmental conditions either in spaceflight microgravity on the International Space Station, termed Flight (FL) samples, or at Earth-normal gravity, termed Ground Control (GC) samples. Susceptibility of FL and GC samples was compared to 72 antibiotics and growth-inhibitory compounds using the Omnilog Phenotype Microarray (PM) system...
August 18, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28821222/impact-of-blood-collection-and-processing-on-peripheral-blood-gene-expression-profiling-in-type-1-diabetes
#17
Linda Yip, Rebecca Fuhlbrigge, Mark A Atkinson, C Garrison Fathman
BACKGROUND: The natural history of type 1 diabetes (T1D) is challenging to investigate, especially as pre-diabetic individuals are difficult to identify. Numerous T1D consortia have been established to collect whole blood for gene expression analysis from individuals with or at risk to develop T1D. However, with no universally accepted protocol for their collection, differences in sample processing may lead to variances in the results. Here, we examined whether the choice of blood collection tube and RNA extraction kit leads to differences in the expression of genes that are changed during the progression of T1D, and if these differences could be minimized by measuring gene expression directly from the lysate of whole blood...
August 18, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28820329/sources-to-variability-in-circulating-human-mirna-signatures
#18
Andreas Keller, Trine Rounge, Christina Backes, Nicole Ludwig, Randi Gislefoss, Petra Leidinger, Hilde Langseth, Eckart Meese
An increasing number of studies propose circulating microRNAs (miRNAs) as biomarkers for a large number of human diseases including cancer, cardiovascular diseases, neurological pathologies and others. To further validate miRNA as biomarkers it is indispensable to understand the variability of circulating miRNAs in healthy individuals. We determined the longitudinal miRNomes of 90 serum samples from the Janus Serum Bank in Norway, which have been stored between 23 and 40 years at -25 degrees Celsius. We profiled three serum samples with microarrays for 30 individuals, each...
August 18, 2017: RNA Biology
https://www.readbyqxmd.com/read/28819626/robust-significance-analysis-of-microarrays-by-minimum-%C3%AE-divergence-method
#19
Md Shahjaman, Nishith Kumar, Md Manir Hossain Mollah, Md Shakil Ahmed, Anjuman Ara Begum, S M Shahinul Islam, Md Nurul Haque Mollah
Identification of differentially expressed (DE) genes with two or more conditions is an important task for discovery of few biomarker genes. Significance Analysis of Microarrays (SAM) is a popular statistical approach for identification of DE genes for both small- and large-sample cases. However, it is sensitive to outlying gene expressions and produces low power in presence of outliers. Therefore, in this paper, an attempt is made to robustify the SAM approach using the minimum β-divergence estimators instead of the maximum likelihood estimators of the parameters...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28819573/an-interstitial-4q-deletion-with-a-mosaic-complementary-ring-chromosome-in-a-child-with-dysmorphism-linear-skin-pigmentation-and-hepatomegaly
#20
J Carter, H Brittain, D Morrogh, N Lench, J J Waters
Interstitial deletions of 4q are rarely reported, vary in size, and have limited genotype-phenotype correlations. Here, genome-wide array CGH analysis identified a 21.6 Mb region of copy number loss at 4q12-q21.1 in a patient diagnosed with dysmorphism, linear skin pigmentation, and hepatomegaly. An additional small ring chromosome was detected in 5/30 cells examined via G-banding. Confirmation of the origin of the ring chromosome was obtained by FISH analysis which identified that the ring chromosome contained material from the deleted region of chromosome 4 and was therefore complementary to the 21...
2017: Case Reports in Genetics
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