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https://www.readbyqxmd.com/read/29684858/a-novel-triazine-ring-compound-md568-exerts-in-vivo-and-in-vitro-effects-on-lipid-metabolism
#1
Dan Jia, Ziwen Li, Ying Gao, Yifan Feng, Weimin Li
In this study, we investigated the toxicity and biological activity of a novel triazine ring compound named magnolol derivative 568 (MD568). The LD50 of MD568 was determined in Sprague-Dawley (SD) rats by intraperitoneal injection. Treatment of high fat diet-induced obese rats with MD568 demonstrated that MD568 displayed significant anti-obesity effects. MD568 markedly reduced body weight, blood lipid contents, white adipose tissue (WAT) and liver mass in obese rats. In addition, MD568 modulated lesions in WAT through regulating PPAR-Ɣ, C/EBP-a, SREBP-1c and FABP-4 proteins in the liver through regulating PPAR-α, SREBP-1c and FABP-4...
April 20, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29684759/using-structurally-defined-oligosaccharides-to-understand-the-interactions-between-proteins-and-heparan-sulfate
#2
REVIEW
Ding Xu, Katelyn Arnold, Jian Liu
Heparan sulfate (HS) is widely present on the animal cell surface and in the extracellular matrix. HS achieves its biological functions by interacting with proteins to change proteins' conformation, oligomerization state and cellular location. The challenging question to study HS is how to dissect the relationship between the structures of HS and the biological activities. In the past several years, crucial techniques have been developed to overcome this challenge. A novel chemoenzymatic method to synthesize structurally defined HS oligosaccharides has offered a key access to this class of sulfated carbohydrate molecules...
April 20, 2018: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/29684357/genome-wide-somatic-copy-number-alterations-and-mutations-in-high-grade-pancreatic-intraepithelial-neoplasia
#3
Tatsuo Hata, Masaya Suenaga, Luigi Marchionni, Anne Macgregor-Das, Jun Yu, Koji Shindo, Koji Tamura, Ralph H Hruban, Michael Goggins
To obtain a better understanding of the genetic alterations of high-grade pancreatic intraepithelial neoplasia (HG-PanIN), we performed whole-genome copy number analysis using single nucleotide polymorphism microarrays and targeted next-generation sequencing of 11 microdissected HG-PanIN and two low-grade PanIN (LG-PanIN) lesions associated with HG-PanIN. HG-PanIN mutation profiles were compared to those of their associated invasive pancreatic ductal adenocarcinoma. All PanIN lesions harbored somatic KRAS mutations...
April 20, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29684016/correcting-for-batch-effects-in-case-control-microbiome-studies
#4
Sean M Gibbons, Claire Duvallet, Eric J Alm
High-throughput data generation platforms, like mass-spectrometry, microarrays, and second-generation sequencing are susceptible to batch effects due to run-to-run variation in reagents, equipment, protocols, or personnel. Currently, batch correction methods are not commonly applied to microbiome sequencing datasets. In this paper, we compare different batch-correction methods applied to microbiome case-control studies. We introduce a model-free normalization procedure where features (i.e. bacterial taxa) in case samples are converted to percentiles of the equivalent features in control samples within a study prior to pooling data across studies...
April 23, 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29683978/antimicrobial-peptide-human-neutrophil-peptide-1-as-a-potential-link-between-chronic-inflammation-and-ductal-adenocarcinoma-of-the-pancreas
#5
Thomas Pausch, Sarah Adolph, Klaus Felix, Andrea S Bauer, Frank Bergmann, Jens Werner, Werner Hartwig
OBJECTIVES: Defensins are antimicrobial peptides playing a role in innate immunity, in epithelial cell regeneration, and in carcinogenesis of inflammation-triggered malignancies. We analyzed this role in pancreatic ductal adenocarcinoma (PDAC) in the context of its association with chronic pancreatitis (CP). METHODS: Human tissue of healthy pancreas, CP, and PDAC was screened for defensins by immunohistochemistry. Defensin α 1 (human neutrophil peptide 1 [HNP-1]) expression was validated using mass spectrometry and microarray analysis...
April 19, 2018: Pancreas
https://www.readbyqxmd.com/read/29683892/implication-of-highly-cytotoxic-natural-killer-cells-for-esophageal-squamous-cell-carcinoma-treatment
#6
Kee Siang Lim, Kosaku Mimura, Ley-Fang Kua, Kensuke Shiraishi, Koji Kono
Esophageal squamous cell carcinoma (ESCC) is an aggressive upper gastrointestinal cancer and effective treatments are limited. Previous studies reported that natural killer (NK) cells expanded by coculturing with K562-mb15-41BBL feeder cells, a genetically modified K562 leukemia cell line that expresses membrane-bound interleukin (IL)-15 and 41BBL ligand, were highly proliferative and highly cytotoxic. Here, we investigated the potential of expanded NK cells for ESCC treatment. We analyzed both genetic and surface expression levels of NKG2D ligands (NKG2DLs) in ESCC using publicly available microarray data sets and ESCC cell lines...
April 20, 2018: Journal of Immunotherapy
https://www.readbyqxmd.com/read/29683265/tissue-based-quantitative-proteomics-to-screen-and-identify-the-potential-biomarkers-for-early-recurrence-metastasis-of-esophageal-squamous-cell-carcinoma
#7
Xu-Wei Cai, Wei-Wei Yu, Wen Yu, Qin Zhang, Wen Feng, Mi-Na Liu, Meng-Hong Sun, Jia-Qing Xiang, Ya-Wei Zhang, Xiao-Long Fu
Esophageal squamous cell carcinoma (ESCC) is the eighth cause of cancer-related deaths worldwide. To screen potential biomarkers associated with early recurrence/metastasis (R/M) of ESCC patients after radical resection, ESCC patients were analyzed by a comparative proteomics analysis using iTRAQ with RPLC-MS to screen differential proteins among R/M groups and adjacent normal tissues. The proteins were identified by qRT-PCR, Western blotting, and tissue microarray. The protein and mRNA expression difference of PHB2 between tumor tissues of ESCC patients and adjacent normal tissues, ESCC patients with and without metastasis, four ESCC cell lines and normal esophageal epithelial cells were inspected using immunohistochemical staining, qRT-PCR, and Western blotting...
April 23, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29683214/piezo1-functions-as-a-potential-oncogene-by-promoting-cell-proliferation-and-migration-in-gastric-carcinogenesis
#8
Jinglin Zhang, Yuhang Zhou, Tingting Huang, Feng Wu, Liping Liu, Johnny S H Kwan, Alfred S L Cheng, Jun Yu, Ka Fai To, Wei Kang
Gastric cancer (GC) is one of the most common malignancies in Asian areas. PIEZO1 has been implied to regulate epithelial homeostasis to play an important role in physiological processes. It is also related to tumor initiation and progression. However, the role of PIEZO1 has not yet been explored in GC. The expression of PIEZO1 in GC cell lines and primary samples was determined by qRT-PCR and Western blot. The clinical correlation of PIEZO1 in GC was evaluated by immunohistochemistry on tissue microarray. The oncogenic role of PIEZO1 was demonstrated in gastric tumorigenesis through a series of functional assays, including cell proliferation, cell invasion and flow cytometry analysis...
April 23, 2018: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29683140/liquid-biopsy-in-the-omics-era-of-tumor-medicine
#9
Yi Jiang, Denong Wang
Liquid biopsy uses noninvasive blood test to assess tumor heterogeneity and evolution in real time. It looks for tumor components in the blood circulation, such as circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA), to provide tumor-specific information. By detecting multiplex tumor biomarkers, including nucleic acids, proteins, carbohydrates, and other tumor-derived substances, liquid biopsy helps with early tumor diagnosis, tumor evolution monitoring, and prognosis prediction. With the development of high-throughput OMICS tools like carbohydrate microarray and high-speed fiber-optic array scanning technology (FAST scan), it is now practical to identify glycan markers of CTCs and cancer stem cells (CSCs), especially those that are cell-surface exposed and readily accessible for immune recognition and targeting...
2018: Open Access J Biomed Eng Appl
https://www.readbyqxmd.com/read/29682874/site-specific-surface-functionalization-via-microchannel-cantilever-spotting-%C3%A2%C2%B5cs-comparison-between-azide-alkyne-and-thiol-alkyne-click-chemistry-reactions
#10
Seyed Mohammad Mahdi Dadfar, Sylwia Sekula-Neuner, Uwe Bog, Vanessa Trouillet, Michael Hirtz
Different types of click chemistry reactions are proposed and used for the functionalization of surfaces and materials, and covalent attachment of organic molecules. In the present work, two different catalyst-free click approaches, namely azide-alkyne and thiol-alkyne click chemistry are studied and compared for the immobilization of microarrays of azide or thiol inks on functionalized glass surfaces. For this purpose, the surface of glass is first functionalized with dibenzocyclooctyne-acid (DBCO-acid), a cyclooctyne with a carboxyl group...
April 22, 2018: Small
https://www.readbyqxmd.com/read/29682797/mouse-urothelial-genes-associated-with-voiding-behavior-changes-after-ovariectomy-and-bladder-lipopolysaccharide-exposure
#11
Marian Acevedo-Alvarez, Judy Yeh, Lery Alvarez-Lugo, Ming Lu, Nitin Sukumar, Warren G Hill, Toby C Chai
AIMS: Symptoms from overactive bladder (OAB) and cystitis secondary to urinary tract infection (UTI) can be similar in post-menopausal women. Effects of ovariectomy (OVX) on voiding behavior after lipopolysaccharide (LPS) intravesical exposure (surrogate for cystitis) in mice were measured. Urothelial genes associated with micturition changes were identified. METHODS: Female C57BL6/J mice underwent OVX or sham surgeries (n = 10 for each). Voiding spot assays (VSA) were performed prior to surgery, 4 weeks post-surgery, and each time after 3 consecutive days of transurethral instillation of LPS...
April 23, 2018: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/29682671/gene-expression-data-and-ftir-spectra-provide-a-similar-phenotypic-description-of-breast-cancer-cell-lines-in-2d-and-3d-cultures
#12
Margarita Smolina, Erik Goormaghtigh
Thirteen breast cancer cell lines were grown in traditional two-dimensional (2D) monolayer and three-dimensional (3D) laminin-rich extracellular matrix (lrECM) culture models. Microarray-based transcriptional profiling data were published for these cell lines under both culture conditions. Colonies embedded in Matrigel matrix were fixed in formalin, embedded in paraffin and cut into 4 μm thick sections. The sections were mounted onto infrared-transparent barium fluoride windows and deparaffinized for Fourier transform infrared (FTIR) imaging...
April 23, 2018: Analyst
https://www.readbyqxmd.com/read/29682213/pharmacogenetics-based-area-under-curve-model-can-predict-efficacy-and-adverse-events-from-axitinib-in-individual-patients-with-advanced-renal-cell-carcinoma
#13
Yoshiaki Yamamoto, Ryouichi Tsunedomi, Yusuke Fujita, Toru Otori, Mitsuyoshi Ohba, Yoshihisa Kawai, Hiroshi Hirata, Hiroaki Matsumoto, Jun Haginaka, Shigeo Suzuki, Rajvir Dahiya, Yoshihiko Hamamoto, Kenji Matsuyama, Shoichi Hazama, Hiroaki Nagano, Hideyasu Matsuyama
We investigated the relationship between axitinib pharmacogenetics and clinical efficacy/adverse events in advanced renal cell carcinoma (RCC) and established a model to predict clinical efficacy and adverse events using pharmacokinetic and gene polymorphisms related to drug metabolism and efflux in a phase II trial. We prospectively evaluated the area under the plasma concentration-time curve (AUC) of axitinib, objective response rate, and adverse events in 44 consecutive advanced RCC patients treated with axitinib...
March 30, 2018: Oncotarget
https://www.readbyqxmd.com/read/29682201/molecular-signatures-associated-with-tumor-specific-immune-response-in-melanoma-patients-treated-with-dendritic-cell-based-immunotherapy
#14
Tamara García-Salum, Andrea Villablanca, Franziska Matthäus, Andrés Tittarelli, Mauricio Baeza, Cristián Pereda, M Alejandra Gleisner, Fermín E González, Mercedes N López, Jörg D Hoheisel, Johannes Norgauer, Peter J Gebicke-Haerter, Flavio Salazar-Onfray
Purpose: We previously showed that autologous dendritic cells (DCs) loaded with an allogeneic heat shock (HS)-conditioned melanoma cell-derived lysate, called TRIMEL, induce T-cell-mediated immune responses in stage IV melanoma patients. Importantly, a positive delayed-type hypersensitivity (DTH) reaction against TRIMEL after vaccination, correlated with patients prolonged survival. Furthermore, we observed that DTH reaction was associated with a differential response pattern reflected in the presence of distinct cell subpopulations in peripheral blood...
March 30, 2018: Oncotarget
https://www.readbyqxmd.com/read/29682102/expression-of-pentose-phosphate-pathway-related-proteins-in-breast-cancer
#15
Junjeong Choi, Eun-Sol Kim, Ja Seung Koo
Purpose: The purpose of this study was to assess the expression of pentose phosphate pathway- (PPP-) related proteins and their significance in clinicopathologic factors of breast cancer. Methods: Immunohistochemical staining for PPP-related proteins (glucose-6-phosphate dehydrogenase [G6PDH], 6-phosphogluconolactonase [6PGL], 6-phosphogluconate dehydrogenase [6PGDH], and nuclear factor-erythroid 2-related factor 2 [NRF2]) was performed using tissue microarray (TMA) of 348 breast cancers...
2018: Disease Markers
https://www.readbyqxmd.com/read/29681762/correlation-of-high-risk-soft-tissue-sarcoma-biomarker-expression-patterns-with-outcome-following-neoadjuvant-chemoradiation
#16
John M Kane, Anthony Magliocco, Qiang Zhang, Dian Wang, Alex Klimowicz, Jonathan Harris, Jeff Simko, Thomas DeLaney, William Kraybill, David G Kirsch
Background: Sarcoma mortality remains high despite adjuvant chemotherapy. Biomarker predictors of treatment response and outcome could improve treatment selection. Methods: Tissue microarrays (TMAs) were created using pre- and posttreatment tumor from two prospective trials (MGH pilot and RTOG 9514) of neoadjuvant/adjuvant MAID chemotherapy and preoperative radiation. Biomarkers were measured using automated computerized imaging (AQUA or ACIS). Expression was correlated with disease-free survival (DFS), distant disease-free survival (DDFS), and overall survival (OS)...
2018: Sarcoma
https://www.readbyqxmd.com/read/29681158/proteomic-analysis-of-cancer-associated-fibroblasts-reveal-a-paracrine-role-for-mfap5-in-human-oral-tongue-squamous-cell-carcinoma
#17
Simona Principe, Salvador Mejia-Guerrero, Vladimir Ignatchenko, Ankit Sinha, Alex Ignatchenko, Wei Shi, Keira Pereira, Susie Su, Shao Hui Huang, Brian O'Sullivan, Wei Xu, David Goldstein, Ilan Weinreb, Laurie Ailles, Fei-Fei Liu, Thomas Kislinger
Bidirectional communication between cells and their microenvironment is crucial for both normal tissue homeostasis and tumor growth. During the development of oral tongue squamous cell carcinoma (OTSCC), cancer-associated fibroblasts (CAFs) create a supporting niche by maintaining a bidirectional crosstalk with cancer cells, mediated by classically secreted factors and various nanometer-sized vesicles, termed as extracellular vesicles (EVs). To better understand the role of CAFs within the tumor stroma and elucidate the mechanism by which secreted proteins contribute to OTSCC progression, we isolated and characterized patient-derived CAFs from resected tumors with matched adjacent tissue fibroblasts (AFs)...
April 22, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29681104/whole-exome-sequencing-and-chromosomal-microarray-analysis-feasible-and-cost-effective-in-an-underserved-population-whole-exome-sequencing-and-chromosomal-microarray-analysis-are-more-expensive-up-front-but-are-far-more-effective-in-making-a-genetic-diagnosis
#18
https://www.readbyqxmd.com/read/29680877/role-of-arabidopsis-abf1-3-4-during-det1-germination-in-salt-and-osmotic-stress-conditions
#19
V C Dilukshi Fernando, Wesam Al Khateeb, Mark F Belmonte, Dana F Schroeder
Arabidopsis det1 mutants exhibit salt and osmotic stress resistant germination. This phenotype requires HY5, ABF1, ABF3, and ABF4. While DE-ETIOLATED 1 (DET1) is well known as a negative regulator of light development, here we describe how det1 mutants also exhibit altered responses to salt and osmotic stress, specifically salt and mannitol resistant germination. LONG HYPOCOTYL 5 (HY5) positively regulates both light and abscisic acid (ABA) signalling. We found that hy5 suppressed the det1 salt and mannitol resistant germination phenotype, thus, det1 stress resistant germination requires HY5...
April 21, 2018: Plant Molecular Biology
https://www.readbyqxmd.com/read/29680858/relationship-between-histone-deacetylase-3-hdac3-and-breast-cancer
#20
Zhuhong Cui, Mingjun Xie, Zhenru Wu, Yujun Shi
BACKGROUND The modification of histone acetylation and deacetylation is the most important mechanism of chromatin remodeling. These modifications are a subset of epigenetic alterations which affect tumorigenesis and progression through changes in gene expression and cell growth. Results of histone modification studies prompted us to explore the therapeutic and prognostic significance of histone deacetylase 3 (HDAC3) expression in patients with breast cancer. MATERIAL AND METHODS Immunohistochemical (IHC) staining was used to detect HDAC3 expression in a tissue microarray (TMA) that included 145 patients diagnosed with invasive ductal breast carcinoma...
April 22, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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