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https://www.readbyqxmd.com/read/28732356/the-circrna-interactome-innovative-hallmarks-of-the-intra-and-extracellular-radiation-response
#1
Valerie Bríd O'Leary, Jan Smida, Martina Matjanovski, Corinna Brockhaus, Klaudia Winkler, Simone Moertl, Saak Victor Ovsepian, Michael John Atkinson
Generated by Quaking (QKI), circular RNAs (circRNAs) are newly recognised non-coding RNA (ncRNA) members characterised by tissue specificity, increased stability and enrichment within exosomes. Studies have shown that ionizing radiation (IR) can influence ncRNA transcription. However, it is unknown whether circRNAs or indeed QKI are regulated by IR. Microarray circRNA profiling and next generation sequencing revealed that circRNA expression was altered by low and medium dose exposure sourced predominantly from genes influencing the p53 pathway...
July 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28732184/high-throughput-identification-of-mir-596-inducing-p53-mediated-apoptosis-in-hela-and-hct116-cells-using-cell-microarray
#2
Ming Ma, Junyu Yang, Bolun Wang, Zhihua Zhao, Jianzhong Jeff Xi
miRNAs play a key role in the regulation of gene networks in mammalian cells. However, little is known about their roles and functions in the apoptosis pathway. Here, we conducted a whole-genome miRNA screening for apoptosis and identified more than 100 miRNAs as apoptosis inducers. To further explain the roles of these mRNAs in apoptosis, a second round of screening was conducted between p53 +/+ and -/- cells. Among the hits, miR-596 was identified as a regulator of p53. The overexpression of miR-596 significantly increased p53 at the protein level, thereby inducing apoptosis...
July 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/28731868/inflammatory-myofibroblastic-tumors-of-the-female-genital-tract-are-under-recognized-a-low-threshold-for-alk-immunohistochemistry-is-required
#3
Justine L Pickett, Angela Chou, Juliana A Andrici, Adele Clarkson, Loretta Sioson, Amy Sheen, Jessica Reagh, Fedaa Najdawi, Yoomee Kim, Denise Riley, Jayne Maidens, David Nevell, Kirsten McIlroy, Susan Valmadre, Greg Gard, Russell Hogg, John Turchini, Gregory Robertson, Michael Friedlander, Anthony J Gill
Inflammatory myofibroblastic tumor (IMT) of the female genital tract is under-recognized. We investigated the prevalence of ALK-positive IMT in lesions previously diagnosed as gynecologic smooth muscle tumors. Immunohistochemistry (IHC) for ALK was performed on tissue microarrays of unselected tumors resected from 2009 to 2013. Three of 1176 (0.26%) "leiomyomas" and 1 of 44 (2.3%) "leiomyosarcomas" were ALK IHC positive, confirmed translocated by fluorescence in situ hybridization (FISH) and therefore more appropriately classified as IMT...
July 20, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28731711/identification-of-proteomic-features-to-distinguish-benign-pulmonary-nodules-from-lung-adenocarcinoma
#4
Simona G Codreanu, Megan D Hoeksema, Robbert J C Slebos, Lisa J Zimmerman, S M Jamshedur Rahman, Ming Li, Sheau-Chiann Chen, Heidi Chen, Rosana Eisenberg, Daniel C Liebler, Pierre P Massion
We hypothesized that distinct protein expression features of benign and malignant pulmonary nodules may reveal novel candidate biomarkers for the early detection of lung cancer. We performed proteome profiling by liquid chromatography-tandem mass spectrometry to characterize 34 resected benign lung nodules, 24 early stage untreated lung adenocarcinomas (ADCs) and biopsies of bronchial epithelium. Group comparisons identified 65 proteins that differentiate nodules from ADCs and normal bronchial epithelium, and 66 proteins that differentiate ADCs from nodules and normal bronchial epithelium...
July 21, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28731290/autoantibodies-against-pfdn2-are-associated-with-an-increased-risk-of-type-2-diabetes-a-case-control-study
#5
Douglas C Chang, Paolo Piaggi, Robert L Hanson, William C Knowler, Clifton Bogardus, Jonathan Krakoff
BACKGROUND: The adaptive immune system is involved in type 2 diabetes mellitus (T2DM), indicating the presence of unidentified autoantibodies that might be useful biomarkers for emerging immunomodulatory therapy. A prior microarray study with a small number of participants suggested the association of novel autoantibodies with T2DM in Southwest American Indians (SAIs). We therefore sought to determine whether antibodies against 14 target proteins are associated with T2DM in a large case-control study...
July 21, 2017: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/28731191/carboxy-terminus-hsc70-interacting-protein-exerts-a-tumor-inhibition-function-in-head-and-neck-cancer
#6
Meng Xiao, Ming Yan, Jianjun Zhang, Qin Xu, Wantao Chen
Several independent studies have reported the roles of the E3 ubiquitin ligase, carboxy-terminus Hsc70 interacting protein (CHIP) in various types of cancers. However, the biological effects of CHIP vary in regards to different cancers, and the role of CHIP in head and neck cancers (HNCs) remains unknown. In the present study, CHIP overexpression plasmids and CHIP knockdown lentivirus were constructed to affect the expression levels of CHIP protein and biological behaviors in HNC cell lines bilaterally. The biological behaviors regulated by CHIP in HNCs were investigated both in vivo and in vitro with a series of assays and analyses...
July 17, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28731166/screening-of-the-prognostic-targets-for-breast-cancer-based-co-expression-modules-analysis
#7
Huijuan Liu, Hui Ye
The purpose of the present study was to screen the prognostic targets for breast cancer based on a co-expression modules analysis. The microarray dataset GSE73383 was downloaded from the Gene Expression Omnibus (GEO) database, including 15 breast cancer samples with good prognosis and 9 breast cancer samples with poor prognosis. The differentially expressed genes (DEGs) were identified with the limma package. The Database for Annotation, Visualization and Integrated Discovery was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis...
July 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28731161/investigation-of-the-molecular-mechanisms-underlying-myotonic-dystrophy-types-1-and-2-cataracts-using-microrna%C3%A2-target-gene-networks
#8
Dewang Shao, Xiaoquan Zhu, Wei Sun, Lu Huo, Wei Chen, Hua Wang, Bing Liu, Peng Pan
The purpose of the present study was to investigate the molecular mechanisms of myotonic dystrophy (DM) 1 and 2 cataracts using bioinformatics methods. A microarray dataset (E‑MEXP‑3365) downloaded from the Array Express database included lens epithelial samples of DM1 and DM2 cataract patients (n=3/group) and non‑DM lens epithelial samples as a control (n=4). Differentially expressed genes (DEGs) were identified between DM1 and control samples, and between DM2 and control samples. Pathway enrichment analyses were performed for the DEGs...
July 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28731140/integrated-analysis-of-genome%C3%A2-wide-gene-expression-and-dna-methylation-microarray-of-diffuse-large-b%C3%A2-cell-lymphoma-with-tet-mutations
#9
Pengfei Liu, Wenhua Jiang, Jinkun Zhao, Huilai Zhang
Diffuse large B-cell lymphoma (DLBCL), the most frequently occurring type of lymphoid malignancy, has been demonstrated to be associated with mutations of Ten‑Eleven Translocation (TET). In order to explore the association between DLBCL and TET mutations, the present study analyzed the gene expression and methylation profiles in human DLBCL biopsy tissues with wildtype and mutated TET2. The microarray dataset GSE37365, containing two subseries: the genome‑wide gene expression dataset GSE37362 and the DNA methylation microarray dataset GSE37363, was downloaded from the Gene Expression Omnibus database...
July 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28731128/lncrna-expression-character-associated-with-ischemic-reperfusion-injury
#10
Xiaowei Wu, Hongyi Zhu, Suhua Zhu, Maojuan Hao, Qingping Li
Ischemic reperfusion injury (IRI) contributes to morbidity and mortality worldwide and results in a poor outcome for patients suffering from myocardial infarction. Ischemic post‑conditioning (IPostC), consisting of one or several brief periods of ischemia and reperfusion, generates powerful protection against IRI. The mechanism of IPostC initiation and development has previously been investigated, however still remains to be fully elucidated. Notably, long non‑coding (lnc) RNAs have previously been demonstrated to be important in cardiovascular diseases...
July 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28731125/n-peptide-of-vmip-%C3%A2-reverses-paclitaxel-resistance-by-regulating-mirna-335-in-breast-cancer
#11
Yangyang Wang, Haifeng Wang, Yongxing Ding, Yu Li, Sulian Chen, Lingyu Zhang, Haihua Wu, Jihong Zhou, Kecai Duan, Wenrui Wang, Changjie Chen, Qingling Yang
Acquisition of resistance to paclitaxel is one of the most important problems in treatment of breast cancer patients, but the molecular mechanisms underlying sensitivity to paclitaxel remains elusive. Emerging evidence has demonstrated that microRNAs (miRNAs) play important roles in regulation of cell growth, migration and invasion through inhibiting the expression of its target genes. In our previous studies, we have shown that microRNA-335 (miR‑335) decreased obviously between paclitaxel-resistant (PR) and parental breast cancer cells through miRNA microarray...
July 19, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28731046/pd-l1-expression-and-the-immune-microenvironment-in-primary-invasive-lobular-carcinomas-of-the-breast
#12
Elizabeth D Thompson, Janis M Taube, Rebecca J Asch-Kendrick, Aleksandra Ogurtsova, Haiying Xu, Rajni Sharma, Alan Meeker, Pedram Argani, Leisha A Emens, Ashley Cimino-Mathews
Tumor-infiltrating lymphocytes and immune checkpoint proteins such as PD-L1 are potential prognostic factors and therapeutic targets in breast cancer. Most studies characterizing the breast tumor immune microenvironment have focused on ductal carcinomas. Here we investigate the tumor microenvironment of primary invasive lobular carcinomas. Previously constructed tissue microarrays of 47 lobular carcinomas were labeled by immunohistochemistry for PD-L1, CD8, CD20, and FoxP3. The stromal immune infiltrate density was qualitatively scored as a percentage of tumor area: 1+ (<5%); 2+ (5-10%); 3+ (10-15%); or 4+ (>50%)...
July 21, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28730788/topoisomerase-i-deregulation-in-laryngeal-squamous-cell-carcinomas-based-on-tissue-microarray-analysis
#13
Theodoros A Papadas, Evangelos Tsiambas, Nicholas S Mastronikolis, Andreas Karameris, Stylianos N Mastronikolis, Athanasios T Papadas, Panagiotis P Fotiades, Vasileios Ragos
PURPOSE: Topoisomerases (types: I/IIa-b/IIIa-b) represent a super-family of nucleic enzymes involved in the DNA replication, transcription, recombination, and also chromosome topological formation. Topoisomerase's I (Topo I- gene location: 20q12) aberrant expression is a frequent genetic event in a variety of solid malignancies. Topo I inhibition promotes cell death due to DNA damage and for this reason it is a target for specific targeted chemotherapy (camptothecin, topotecan, irinotecan)...
May 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28730556/stem-cell-derived-retinal-pigment-epithelium-the-role-of-pigmentation-as-maturation-marker-and-gene-expression-profile-comparison-with-human-endogenous-retinal-pigment-epithelium
#14
A Bennis, J G Jacobs, L A E Catsburg, J B Ten Brink, C Koster, R O Schlingemann, J van Meurs, T G M F Gorgels, P D Moerland, V M Heine, A A Bergen
In age-related macular degeneration (AMD) the retinal pigment epithelium (RPE) deteriorates, leading to photoreceptor decay and severe vision loss. New therapeutic strategies aim at RPE replacement by transplantation of pluripotent stem cell (PSC)-derived RPE. Several protocols to generate RPE have been developed where appearance of pigmentation is commonly used as indicator of RPE differentiation and maturation. It is, however, unclear how different pigmentation stages reflect developmental stages and functionality of PSC-derived RPE cells...
July 21, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28729771/microarray-gene-expression-analysis-of-chemosensitivity-for-docetaxel-cisplatin-and-5-fluorouracil-tpf-combined-chemotherapeutic-regimen-in-hypopharyngeal-squamous-cell-carcinoma
#15
Meng Lian, Haizhou Wang, Jugao Fang, Jie Zhai, Ru Wang, Xixi Shen, Yifan Yang, Zhihong Ma, Honggang Liu
OBJECTIVE: To screen out a set of candidate genes which could help to determine whether patients with hypopharyngeal squamous cell carcinoma (HSCC) could benefit from docetaxel, cisplatin and 5-fluorouracil (TPF) induction chemotherapy. METHODS: Gene-expression profiles in 12 TPF-sensitive patients were compared to 9 resistant controls by microarray analysis. Subsequently, expression levels of potential biomarkers in chemosensitive cell line FaDu after TPF treatment were observed by quantitative real-time polymerase chain reaction (qRT-PCR)...
June 2017: Chinese Journal of Cancer Research, Chung-kuo Yen Cheng Yen Chiu
https://www.readbyqxmd.com/read/28729721/plasma-derived-exosome-characterization-reveals-a-distinct-microrna-signature-in-long-duration-type-1-diabetes
#16
Marta Garcia-Contreras, Sanket H Shah, Alejandro Tamayo, Paul D Robbins, Ronald B Golberg, Armando J Mendez, Camillo Ricordi
Type 1 diabetes mellitus (T1DM) results from an autoimmune attack against the insulin-producing ß cells which leads to chronic hyperglycemia. Exosomes are lipid vesicles derived from cellular multivesicular bodies that are enriched in specific miRNAs, potentially providing a disease-specific diagnostic signature. To assess the value of exosome miRNAs as biomarkers for T1DM, miRNA expression in plasma-derived exosomes was measured. Nanoparticle tracking analysis and transmission electron microscopy confirmed the presence of plasma-derived exosomes (EXOs) isolated by differential centrifugation...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729702/structure-of-general-population-antibody-titer-distributions-to-influenza-a-virus
#17
Nguyen Thi Duy Nhat, Stacy Todd, Erwin de Bruin, Tran Thi Nhu Thao, Nguyen Ha Thao Vy, Tran Minh Quan, Dao Nguyen Vinh, Janko van Beek, Pham Hong Anh, Ha Minh Lam, Nguyen Thanh Hung, Nguyen Thi Le Thanh, Huynh Le Anh Huy, Vo Thi Hong Ha, Stephen Baker, Guy E Thwaites, Nguyen Thi Nam Lien, Tran Thi Kim Hong, Jeremy Farrar, Cameron P Simmons, Nguyen Van Vinh Chau, Marion Koopmans, Maciej F Boni
Seroepidemiological studies aim to understand population-level exposure and immunity to infectious diseases. Their results are normally presented as binary outcomes describing the presence or absence of pathogen-specific antibody, despite the fact that many assays measure continuous quantities. A population's natural distribution of antibody titers to an endemic infectious disease may include information on multiple serological states - naiveté, recent infection, non-recent infection, childhood infection - depending on the disease in question and the acquisition and waning patterns of immunity...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729529/a-novel-matrix-protein-pfy2-functions-as-a-crucial-macromolecule-during-shell-formation
#18
Yi Yan, Dong Yang, Xue Yang, Chuang Liu, Jun Xie, Guilan Zheng, Liping Xie, Rongqing Zhang
Biomineralization, including shell formation, is dedicatedly regulated by matrix proteins. PfY2, a matrix protein detected in the ethylene diamine tetraacetic acid (EDTA)-soluble fraction from both prismatic layer and nacreous layer, was discovered by our group using microarray. It may play dual roles during biomineralization. However, the molecular mechanism is still unclear. In this research, we studied the function of PfY2 on crystallization in vivo and in vitro, revealing that it might be a negative regulator during shell formation...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729194/establishment-of-human-retinal-mitoscriptome-gene-expression-signature-for-diabetic-retinopathy-using-cadaver-eyes
#19
Gowthaman Govindarajan, Saumi Mathews, Karthik Srinivasan, Kim Ramasamy, Sundaresan Periyasamy
Diabetic retinopathy (DR) is a leading cause of blindness due to retinal microvasculature. We used microarray analysis for the first time to establish the retinal mitoscriptome gene expression signature for DR using human cadaver eyes. Among the 1042 genes, 60 (52-down, 8-up) and 39 (36-down, 3-up) genes were differentially expressed in the DR as compared to normal control and diabetic retinas respectively. These genes were mainly responsible for regulating angiogenesis, anti-oxidant defense mechanism, ATP production and apoptosis contributing to the disease pathology of DR...
July 17, 2017: Mitochondrion
https://www.readbyqxmd.com/read/28728341/dna-microarray-technology-in-hbv-genotyping
#20
Payam Behzadi, Elham Behzadi, Seyed M Alavian
No abstract text is available yet for this article.
October 2017: Minerva Medica
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