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https://www.readbyqxmd.com/read/28523826/occurrence-and-abundance-of-ammonia-oxidizing-archaea-and-bacteria-from-the-surface-to-below-the-water-table-in-deep-soil-and-their-contributions-to-nitrification
#1
Lei Zheng, Xue Zhao, Guibing Zhu, Wei Yang, Chao Xia, Tao Xu
Using molecular biology methods (qualitative and quantitative PCR), we determined the occurrence and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) from a dry inland soil in Basel, Switzerland, and from the riparian zone of Baiyangdian Lake, China. We also determined the contributions of these microorganisms to ammonia oxidization at different depths based on the nitrification rate. The number of archaeal amoA genes (the key functional gene in AOA) was larger than the number of bacterial amoA genes in each sample, suggesting a dominant role for the AOA amoA gene in environments with a low ammonium concentration...
May 19, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28509835/changes-by-the-accreditation-council-for-graduate-medical-education-success-or-failure-an-aoa-critical-issues-symposium
#2
Terry L Thompson, Rick W Wright, Thomas J Nasca
The Accreditation Council for Graduate Medical Education has introduced far-reaching changes to the educational requirements for postgraduate trainees over the past 15 years. These steps were taken to improve the quality of education and to enhance patient safety. This symposium will explore the design and implementation of these changes and determine if they are succeeding.
May 17, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28499263/effect-of-fumigation-with-chloropicrin-on-soil-bacterial-communities-and-genes-encoding-key-enzymes-involved-in-nitrogen-cycling
#3
Jun Li, Bin Huang, Qiuxia Wang, Yuan Li, Wensheng Fang, Dongdong Yan, Meixia Guo, Aocheng Cao
Chloropicrin (CP) is a potential alternative for methyl bromide as a soil fumigant given that the use of methyl bromide has become limited. However, little is known about how fumigation with CP affects the condition of the soil microbial community. In this study, 16S rRNA amplicon sequencing and quantitative PCR were combined to investigate the effect of CP on soil bacterial community. In total, 938,922 effective reads were obtained from 18 samples and clustered into 58,662 operational taxonomic units at a similarity cut-off of 97%...
May 9, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28487691/microbial-community-response-on-wastewater-discharge-in-boreal-lake-sediments
#4
Jatta Saarenheimo, Sanni L Aalto, Antti J Rissanen, Marja Tiirola
Despite high performance, municipal wastewater treatment plants (WWTPs) still discharge significant amounts of organic material and nitrogen and even microbes into the receiving water bodies, altering physico-chemical conditions and microbial functions. In this study, we examined how nitrified wastewater affects the microbiology of boreal lake sediments. Microbial community compositions were assessed with next generation sequencing of the 16S rRNA gene, and a more detailed view on nitrogen transformation processes was gained with qPCR targeting on functional genes (nirS, nirK, nosZI, nosZII, amoAarchaea, and amoAbacteria)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28487508/biochar-amendment-reduces-paddy-soil-nitrogen-leaching-but-increases-net-global-warming-potential-in-ningxia-irrigation-china
#5
Yongsheng Wang, Yansui Liu, Ruliang Liu, Aiping Zhang, Shiqi Yang, Hongyuan Liu, Yang Zhou, Zhengli Yang
The efficacy of biochar as an environmentally friendly agent for non-point source and climate change mitigation remains uncertain. Our goal was to test the impact of biochar amendment on paddy rice nitrogen (N) uptake, soil N leaching, and soil CH4 and N2O fluxes in northwest China. Biochar was applied at four rates (0, 4.5, 9 and13.5 t ha(-1) yr(-1)). Biochar amendment significantly increased rice N uptake, soil total N concentration and the abundance of soil ammonia-oxidizing archaea (AOA), but it significantly reduced the soil NO3(-)-N concentration and soil bulk density...
May 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28476076/effects-of-fumigation-with-metam-sodium-on-soil-microbial-biomass-respiration-nitrogen-transformation-bacterial-community-diversity-and-genes-encoding-key-enzymes-involved-in-nitrogen-cycling
#6
Jun Li, Bin Huang, Qiuxia Wang, Yuan Li, Wensheng Fang, Dawei Han, Dongdong Yan, Meixia Guo, Aocheng Cao
Metam-sodium (MS) is widely used as a soil pre-plant fumigant as methyl bromide is phased out of agriculture. However, the information about how fumigation with MS affects the soil microbial community is still limited. In this study, a 66-day-long experiment was conducted to ascertain the effects of MS on soil substrate-induced respiration (SIR), microbial biomass nitrogen (MBN), NH4(+)-N and NO3(-)-N concentrations, as well as the abundance of the total bacteria and fungi and the expression of genes involved in nitrogen cycling...
May 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28472509/multicenter-phase-ii-study-of-temozolomide-and-myeloablative-chemotherapy-with-autologous-stem-cell-transplant-for-newly-diagnosed-anaplastic-oligodendroglioma
#7
Alissa A Thomas, Lauren E Abrey, Robert Terziev, Jeffrey Raizer, Nina L Martinez, Peter Forsyth, Nina Paleologos, Matthew Matasar, Craig S Sauter, Craig Moskowitz, Stephen D Nimer, Lisa M DeAngelis, Thomas Kaley, Sean Grimm, David N Louis, J Gregory Cairncross, Katherine S Panageas, Samuel Briggs, Geraldine Faivre, Nimish A Mohile, Jayesh Mehta, Philip Jonsson, Debyani Chakravarty, Jianjiong Gao, Nikolaus Schultz, Cameron W Brennan, Jason T Huse, Antonio Omuro
Background: Anaplastic oligodendroglioma (AO) and anaplastic oligoastrocytomas (AOA) are chemotherapy-sensitive tumors with prolonged survival after radiochemotherapy. We report a prospective trial using induction temozolomide (TMZ) followed by myeloablative high-dose chemotherapy (HDC) with autologous stem-cell transplant (ASCT), as a potential strategy to defer radiotherapy. Methods: Patients with AO/AOA received 6 cycles of TMZ (200mg/m2 X5/28-day). Responding patients were eligible for HDC (thiotepa 250mg/m2/day X3 days then busulfan 3...
May 2, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28464016/muc1-facilitates-metabolomic-reprogramming-in-triple-negative-breast-cancer
#8
Gennifer Goode, Venugopal Gunda, Nina V Chaika, Vinee Purohit, Fang Yu, Pankaj K Singh
BACKGROUND: Mucin1 (MUC1), a glycoprotein associated with chemoresistance and an aggressive cancer phenotype, is aberrantly overexpressed in triple-negative breast cancer (TNBC). Recent studies suggest that MUC1 plays a role in modulating cancer cell metabolism and thereby supports tumor growth. Herein, we examined the role of MUC1 in metabolic reprogramming in TNBC. METHODS: MUC1 was stably overexpressed in MDA-MB-231 TNBC cells and stably knocked down in MDA-MB-468 cells...
2017: PloS One
https://www.readbyqxmd.com/read/28463962/nomogram-based-prediction-of-survival-in-patients-with-advanced-oesophagogastric-adenocarcinoma-receiving-first-line-chemotherapy-a-multicenter-prospective-study-in-the-era-of-trastuzumab
#9
A Custodio, A Carmona-Bayonas, P Jiménez-Fonseca, M L Sánchez, A Viudez, R Hernández, J M Cano, I Echavarria, C Pericay, M Mangas, L Visa, E Buxo, T García, A Rodríguez Palomo, F Álvarez Manceñido, A Lacalle, I Macias, A Azkarate, A Ramchandani, A Fernández Montes, C López, F Longo, R Sánchez Bayona, M L Limón, A Díaz-Serrano, A Hurtado, R Madero, C Gómez, J Gallego
BACKGROUND: To develop and validate a nomogram and web-based calculator to predict overall survival (OS) in Caucasian-advanced oesophagogastric adenocarcinoma (AOA) patients undergoing first-line combination chemotherapy. METHODS: Nine hundred twenty-four AOA patients treated at 28 Spanish teaching hospitals from January 2008 to September 2014 were used as derivation cohort. The result of an adjusted-Cox proportional hazards regression was represented as a nomogram and web-based calculator...
May 2, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28463927/population-health-management-is-there-any-role-for-orthopaedics-an-aoa-critical-issues-symposium
#10
Scott D Boden, Brian T Smith, Matthew Handley
The next phase of health-care reform will accelerate the formation of integrated delivery systems and the creation of value and savings through population health management. Accomplishing this goal requires 3 key factors, including (1) enabling groups of physicians and hospitals to legally work together to cover a broad geographic area, (2) the formation of integrated delivery systems that cover the low to high-acuity and post-acute care spectrums, and (3) identifying mechanisms through which a subspecialty can impact the health of a population of patients...
May 3, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28449992/effect-of-incorporating-finger-millet-in-wheat-flour-on-mixolab-behavior-chapatti-quality-and-starch-digestibility
#11
Bharati Sharma, Hardeep Singh Gujral, Vicky Solah
Wheat and finger millet flour (two cultivars) were blended in the ratio (3:1) to form a composite flour and its dough properties were studied on the mixolab. The chapatti making and digestibility behavior of the composite flour was also investigated. The wheat finger millet (WFM) flour blend displayed up to 30.7% higher total phenolic content (TPC), 38.2% higher total flavonoid content (TFC) and 75.4% higher antioxidant activity (AOA) than the wheat flour. Chapattis prepared from the composite blends exhibited lower retrogradation as evident by the mixolab retrogradation index, higher values of soluble starch and soluble amylose in stored chapatti...
September 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28448431/a-type-2-block-component-decomposition-based-2d-aoa-estimation-algorithm-for-an-electromagnetic-vector-sensor-array
#12
Yu-Fei Gao, Guan Gui, Wei Xie, Yan-Bin Zou, Yue Yang, Qun Wan
This paper investigates a two-dimensional angle of arrival (2D AOA) estimation algorithm for the electromagnetic vector sensor (EMVS) array based on Type-2 block component decomposition (BCD) tensor modeling. Such a tensor decomposition method can take full advantage of the multidimensional structural information of electromagnetic signals to accomplish blind estimation for array parameters with higher resolution. However, existing tensor decomposition methods encounter many restrictions in applications of the EMVS array, such as the strict requirement for uniqueness conditions of decomposition, the inability to handle partially-polarized signals, etc...
April 27, 2017: Sensors
https://www.readbyqxmd.com/read/28446904/dynamic-response-of-ammonia-oxidizers-to-four-fertilization-regimes-across-a-wheat-rice-rotation-system
#13
Jichen Wang, Lei Ni, Yang Song, Geoff Rhodes, Jing Li, Qiwei Huang, Qirong Shen
Ammonia oxidation by microorganisms is a rate-limiting step of the nitrification process and determines the efficiency of fertilizer utilized by crops. Little is known about the dynamic response of ammonia-oxidizers to different fertilization regimes in a wheat-rice rotation system. Here, we examined ammonia-oxidizing bacteria (AOB) and archaea (AOA) communities across eight representative stages of wheat and rice growth and under four fertilization regimes: no nitrogen fertilization (NNF), chemical fertilization (CF), organic-inorganic mixed fertilizer (OIMF) and organic fertilization (OF)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28441063/validating-the-psycholinguistic-aspects-of-litmus-clt-evidence-from-polish-and-norwegian
#14
Pernille Hansen, Hanne Gram Simonsen, Magdalena Łuniewska, Ewa Haman
The novel assessment tool Cross-Linguistic Lexical Tasks (LITMUS-CLT) aims for comparable cross-linguistic assessment of multilingual children's lexical skills by basing each language version on two language-specific variables: age of acquisition (AoA) and complexity index (CI), a novel measure related to phonology, morphology, exposure and etymology. This article investigates the validity of this methodology, asking whether the underlying properties are robust predictors of children's performance. The Polish and Norwegian CLTs were used to assess 32 bilingual Polish-Norwegian, 34 monolingual Norwegian and 36 monolingual Polish children...
April 25, 2017: Clinical Linguistics & Phonetics
https://www.readbyqxmd.com/read/28428695/increased-oxidatively-modified-forms-of-albumin-in-association-with-decreased-total-antioxidant-activity-in-different-types-of-hypertensive-disorders-of-pregnancy
#15
Jyothi M P D'souza, Sindhu Harish, Vinitha Ramanath Pai, Chitra Shriyan
Protein oxidation has been demonstrated in preeclampsia, but this finding has not been established in other hypertensive disorders in pregnancy (HDP). The present study comparatively evaluated ischemia modified albumin (IMA) and advanced oxidation protein products (AOPP) in different HDP and investigated their association with total antioxidant activity (AOA) and total thiols. There was a significant increase in AOPP and IMA, a significant decrease in AOA, total thiols and albumin in every HDP compared to controls...
June 2017: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/28419436/ammonia-oxidizer-populations-vary-with-nitrogen-cycling-across-a-tropical-montane-mean-annual-temperature-gradient
#16
S Pierre, I Hewson, J P Sparks, C M Litton, C Giardina, P M Groffman, T J Fahey
Functional gene approaches have been used to better understand the roles of microbes in driving forest soil nitrogen (N) cycling rates and bioavailability. Ammonia oxidation is a rate limiting step in nitrification, and is a key area for understanding environmental constraints on N availability in forests. We studied how increasing temperature affects the role of ammonia oxidizing archaea (AOA) and bacteria (AOB) in soil N cycling and availability by using a highly constrained natural mean annual temperature (MAT) elevation gradient in a tropical montane wet forest...
April 18, 2017: Ecology
https://www.readbyqxmd.com/read/28419042/geriatric-fracture-care-future-trajectories-a-2015-aoa-critical-issues-symposium
#17
Julie A Switzer, Kevin J Bozic, Stephen L Kates
The population of the United States and the world is aging rapidly. Musculoskeletal care for older adults will be impacted by the manner in which health care is financed and the ability of the orthopaedic community to provide evidence-based integrated care for this population. We review the financial aspects of health-care reform and the implications for musculoskeletal care in the elderly. We discuss the establishment of quality measures for hip fracture care in the elderly, team building to accomplish this, and an innovative program designed to provide orthopaedic care to the frail elderly outside of the usual office setting...
April 19, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28394701/nitrogen-removal-and-its-relationship-with-the-nitrogen-cycle-genes-and-microorganisms-in-the-horizontal-subsurface-flow-constructed-wetlands-with-different-design-parameters
#18
Jun Chen, Guang-Guo Ying, You-Sheng Liu, Xiao-Dong Wei, Shuang-Shuang Liu, Liang-Ying He, Yong-Qiang Yang, Fan-Rong Chen
This study aims to investigate nitrogen removal and its relationship with the nitrogen-cycle genes and microorganisms in the horizontal subsurface flow constructed wetlands (CWs) with different design parameters. Twelve mesocosm-scale CWs with four substrates and three hydraulic loading rates were set up in the outdoor. The result showed the CWs with zeolite as substrate and HLR of 20 cm/d were selected as the best choice for the TN and NH3-N removal. It was found that the single-stage mesocosm-scale CWs were incapable to achieve high removals of TN and NH3-N due to inefficient nitrification process in the systems...
April 10, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28390307/comammox-in-drinking-water-systems
#19
Yulin Wang, Liping Ma, Yanping Mao, Xiaotao Jiang, Yu Xia, Ke Yu, Bing Li, Tong Zhang
The discovery of complete ammonia oxidizer (comammox) has fundamentally upended our perception of the global nitrogen cycle. Here, we reported four metagenome assembled genomes (MAGs) of comammox Nitrospira that were retrieved from metagenome datasets of tap water in Singapore (SG-bin1 and SG-bin2), Hainan province, China (HN-bin3) and Stanford, CA, USA (ST-bin4). Genes of phylogenetically distinct ammonia monooxygenase subunit A (amoA) and hydroxylamine dehydrogenase (hao) were identified in these four MAGs...
June 1, 2017: Water Research
https://www.readbyqxmd.com/read/28376730/application-of-hierarchical-oligonucleotide-primer-extension-hope-to-assess-relative-abundances-of-ammonia-and-nitrite-oxidizing-bacteria
#20
Giantommaso Scarascia, Hong Cheng, Moustapha Harb, Pei-Ying Hong
BACKGROUND: Establishing an optimal proportion of nitrifying microbial populations, including ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), complete nitrite oxidizers (comammox) and ammonia-oxidizing archaea (AOA), is important for ensuring the efficiency of nitrification in water treatment systems. Hierarchical oligonucleotide primer extension (HOPE), previously developed to rapidly quantify relative abundances of specific microbial groups of interest, was applied in this study to track the abundances of the important nitrifying bacterial populations...
April 4, 2017: BMC Microbiology
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