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https://www.readbyqxmd.com/read/28327357/the-ternary-complex-structure-of-d-mandelate-dehydrogenase-with-nadh-and-anilino-oxo-acetate
#1
Nayuta Furukawa, Akimasa Miyanaga, Masahiro Nakajima, Hayao Taguchi
Enterococcus faecium NAD-dependent d-mandelate dehydrogenase (D-ManDH) belongs to a ketopantoate reductase (KPR)-related D-2-hydroxyacid dehydrogenase family, and exhibits broad substrate specificity toward bulky hydrophobic 2-ketoacids, preferring C3-branched substrates. The ternary complex structure of D-ManDH with NADH and anilino(oxo)acetate (AOA) revealed that the substrate binding induces a shear motion of the N-terminal domain along the C-terminal domain, following the hinge motion induced by the NADH binding, and allows the bound NADH molecule to form favorable interactions with a 2-ketoacid substrate...
March 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28326893/psycholinguistic-norms-for-320-fixed-expressions-idioms-and-proverbs-in-french
#2
Patrick Bonin, Alain Méot, Jean-Michel Boucheix, Aurélia Bugaiska
We provide psycholinguistic norms for a new set of 160 French idiomatic expressions and 160 proverbs: knowledge, predictability, literality, compositionality, subjective and objective frequency, familiarity, age of acquisition (AoA), and length. Different analyses (reliability, descriptive statistics, correlations) performed on the norms are reported and discussed. The norms can be downloaded as supplemental material.
March 22, 2017: Quarterly Journal of Experimental Psychology: QJEP
https://www.readbyqxmd.com/read/28324256/legacy-effects-of-simulated-short-term-climate-change-on-ammonia-oxidisers-denitrifiers-and-nitrous-oxide-emissions-in-an-acid-soil
#3
Xiaoya Xu, Xiaorui Liu, Yong Li, Yu Ran, Yapeng Liu, Qichun Zhang, Zheng Li, Yan He, Jianming Xu, Hongjie Di
Although the effect of simulated climate change on nitrous oxide (N2O) emissions and on associated microbial communities has been reported, it is not well understood if these effects are short-lived or long-lasting. Here, we conducted a field study to determine the interactive effects of simulated warmer and drier conditions on nitrifier and denitrifier communities and N2O emissions in an acidic soil and the longevity of the effects. A warmer (+2.3 °C) and drier climate (-7.4% soil moisture content) was created with greenhouses...
March 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28319452/the-state-of-the-otolaryngology-match-a-review-of-applicant-trends-impossible-qualifications-and-implications
#4
Sarah N Bowe, Cecelia E Schmalbach, Adrienne M Laury
Objective This State of the Art Review aims (1) to define recent qualifications of otolaryngology resident applicants by focusing on United States Medical Licensing Examination (USMLE) scores, Alpha Omega Alpha (AOA) status, and research/publications and (2) to summarize the current literature regarding the relationship between these measures and performance in residency. Data Sources Electronic Residency Application Service, National Residency Matching Program, PubMed, Ovid, and GoogleScholar. Review Methods Electronic Residency Application Service and National Residency Matching Program data were analyzed to evaluate trends in applicant numbers and qualifications...
March 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28318285/reading-through-the-life-span-individual-differences-in-psycholinguistic-effects
#5
Rob A I Davies, Ruth Arnell, Julia M H Birchenough, Debbie Grimmond, Sam Houlson
The effects of psycholinguistic variables are critical to the evaluation of theories about the cognitive reading system. However, reading research has tended to focus on the impact of key variables on average performance. We report the first investigation examining variation in psycholinguistic effects across the life span, from childhood into old age. We analyzed the performance of a sample of 535 readers, aged 8-83 years in lexical decision and pronunciation tasks. Our findings show that the effects on reading of two key variables, frequency and AoA, decrease in size with increasing age over the life span...
March 20, 2017: Journal of Experimental Psychology. Learning, Memory, and Cognition
https://www.readbyqxmd.com/read/28301365/preserving-the-legitimacy-of-board-certification
#6
Michael S Hanemann, Holly C Wall, John A Dean
OBJECTIVES: The aims of this discussion were to inform the medical community about the American Board of Cosmetic Surgery's ongoing attempts in Louisiana to achieve equivalency to American Board of Medical Specialties (ABMS) member boards so that its diplomates may use the term "board certified" in advertising and to ensure public safety by upholding the standards for medical board certification. BACKGROUND: In 2011, Louisiana passed a truth in medical advertising law, which was intended to protect the public by prohibiting the use of the term "board certified" by improperly credentialed physicians...
March 16, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28283802/nitrogen-metabolism-genes-from-temperate-marine-sediments
#7
Carolina Reyes, Dominik Schneider, Marko Lipka, Andrea Thürmer, Michael E Böttcher, Michael W Friedrich
In this study, we analysed metagenomes along with biogeochemical profiles from Skagerrak (SK) and Bothnian Bay (BB) sediments, to trace the prevailing nitrogen pathways. NO3(-) was present in the top 5 cm below the sediment-water interface at both sites. NH4(+) increased with depth below 5 cm where it overlapped with the NO3(-) zone. Steady-state modelling of NO3(-) and NH4(+) porewater profiles indicates zones of net nitrogen species transformations. Bacterial protease and hydratase genes appeared to make up the bulk of total ammonification genes...
March 10, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28281565/changes-in-land-use-driven-by-urbanization-impact-nitrogen-cycling-and-the-microbial-community-composition-in-soils
#8
Haitao Wang, Christopher W Marshall, Minying Cheng, Huijuan Xu, Hu Li, Xiaoru Yang, Tianling Zheng
Transition of populations from rural to urban living causes landscape changes and alters the functionality of soil ecosystems. It is unclear how this urbanization disturbs the microbial ecology of soils and how the disruption influences nitrogen cycling. In this study, microbial communities in turfgrass-grown soils from urban and suburban areas around Xiamen City were compared to microbial communities in the soils from rural farmlands. The potential N2O emissions, potential denitrification activity, and abundances of denitrifiers were higher in the rural farmland soils compared with the turfgrass soils...
March 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28281465/an-experimental-comparative-study-of-the-efficiency-of-twisted-and-flat-flapping-wings-during-hovering-flight
#9
Hoang Vu Phan, Quang Tri Truong, Hoon Cheol Park
This work presents a parametric study to find a proper wing configuration for achieving economic flight by using unsteady blade element theory, which is based on the three-dimensional kinematics of a flapping wing. Power loading was first considered as a performance parameter for the study. The power loadings at each wing section along the wingspan were obtained for various geometric angles of attacks (AoAs) by calculating the ratios of the vertical forces generated and the powers consumed by that particular wing section...
March 10, 2017: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/28259988/targeting-glutamine-metabolism-and-the-focal-adhesion-kinase-additively-inhibits-the-mammalian-target-of-the-rapamycin-pathway-in-spheroid-cancer-stem-like-properties-of-ovarian-clear-cell-carcinoma-in%C3%A2-vitro
#10
Masakazu Sato, Kei Kawana, Katsuyuki Adachi, Asaha Fujimoto, Mitsuyo Yoshida, Hiroe Nakamura, Haruka Nishida, Tomoko Inoue, Ayumi Taguchi, Juri Ogishima, Satoko Eguchi, Aki Yamashita, Kensuke Tomio, Osamu Wada-Hiraike, Katsutoshi Oda, Takeshi Nagamatsu, Yutaka Osuga, Tomoyuki Fujii
Ovarian cancer is one of the leading causes of death in the world, which is linked to its resistance to chemotherapy. Strategies to overcome chemoresistance have been keenly investigated. Culturing cancer cells in suspension, which results in formation of spheroids, is a more accurate reflection of clinical cancer behavior in vitro than conventional adherent cultures. By performing RNA-seq analysis, we found that the focal adhesion pathway was essential in spheroids. The phosphorylation of focal adhesion kinase (FAK) was increased in spheroids compared to adherent cells, and inhibition of FAK in spheroids resulted in inhibition of the downstream mammalian target of the rapamycin (mTOR) pathway in ovarian clear cell carcinomas...
February 23, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28251112/in-vitro-anticancer-activity-of-ethanolic-extracts-of-piper-nigrum-against-colorectal-carcinoma-cell-lines
#11
Akila Prashant, Chandini Rangaswamy, Anshu Kumar Yadav, Varun Reddy, M N Sowmya, Subbarao Madhunapantula
BACKGROUND: Piper nigrum (PN) is well known for its cytotoxic and pharmacological benefits. However, there is minimal documented evidence about its cytotoxic efficacy against colorectal carcinoma. We therefore sought to procure a precisely quantitative and qualitative result, pertaining the efficacy of an ethanolic extract of PN (EEPN) against colorectal carcinoma. MATERIALS AND METHODS: EEPN was prepared by subjecting dried PN seeds to gradient ethanol fractionation...
January 2017: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/28249125/abundance-and-diversity-of-ammonia-oxidizing-archaea-and-bacteria-in-the-rhizosphere-soil-of-three-plants-in-the-ebinur-lake-wetland
#12
Yuan He, Wenge Hu, Decao Ma, Hongzhu Lan, Yang Yang, Yan Gao
Ammonia oxidation is carried out by ammonia oxidizing bacteria (AOB) and ammonia oxidizing archaea (AOA). The Ebinur Lake wetland is the best example of a temperate arid zone wetland ecosystem in China. Soil samples were collected from rhizosphere and non-rhizosphere soil containing Halocnemum strobilaceum (H and H'), Phragmites australis (R and R'), and Kareliniacaspia (K and K') to study the relationship between environmental factors and the community structure of AOB and AOA. Phylogenetic analysis showed that the AOA sequences belonged to the Nitrosopumilus and Nitrososphaera clusters...
March 1, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28244916/what-s-important-leadership-an-american-orthopaedic-association-aoa-2016-orthotalk
#13
Stuart L Weinstein
No abstract text is available yet for this article.
March 1, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28217688/advancing-community-based-falls-prevention-programs-for-older-adults-the-work-of-the-administration-for-community-living-administration-on-aging
#14
Kristie Kulinski, Casey DiCocco, Shannon Skowronski, Phantane Sprowls
The mission of the Administration for Community Living (ACL) is to maximize the independence, well-being, and health of older adults, people with disabilities across the lifespan, and their families and caregivers. In direct alignment with this mission is ACL's support of evidence-based falls prevention programs in communities throughout the United States. Since 2014, the Administration on Aging (AoA), part of ACL, has invested nearly $14 million in entities such as state agencies, nonprofits, and universities to expand access to proven community-based falls prevention programs...
2017: Frontiers in Public Health
https://www.readbyqxmd.com/read/28211189/influence-of-oxygen-availability-on-the-activities-of-ammonia-oxidizing-archaea
#15
Wei Qin, Kelley A Meinhardt, James W Moffett, Allan H Devol, E Virginia Armbrust, Anitra E Ingalls, David A Stahl
Recent studies point to the importance of oxygen (O2 ) in controlling the distribution and activity of marine ammonia-oxidizing archaea (AOA), one of the most abundant prokaryotes in the ocean. The AOA are associated with regions of low O2 tension in oceanic oxygen minimum zones (OMZs), and O2 availability is suggested to influence their production of the ozone-depleting greenhouse gas nitrous oxide (N2 O). We show that marine AOA available in pure culture sustain high ammonia oxidation activity at low μM O2 concentrations, characteristic of suboxic regions of OMZs (<10 µM O2 ), and that atmospheric concentrations of O2 may inhibit the growth of some environmental populations...
February 17, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28197692/feasibility-of-establishing-an-australian-acl-registry-a-pilot-study-by-the-australian-orthopaedic-association-national-joint-replacement-registry-aoanjrr
#16
Christina Lekkas, Richard Clarnette, Stephen E Graves, Sophia Rainbird, David Parker, Michelle Lorimer, Roger Paterson, Justin Roe, Hayden Morris, Julian A Feller, Peter Annear, Ben Forster, David Hayes
PURPOSE: Rupture of the anterior cruciate ligament (ACL) is a common and debilitating injury that impacts significantly on knee function and risks the development of degenerative arthritis. The outcome of ACL surgery is not monitored in Australia. The optimal treatment is unknown. Consequently, the identification of best practice in treating ACL is crucial to the development of improved outcomes. The Australian Knee Society (AKS) asked the Australian Orthopaedic Association (AOA) to consider establishing a national ACL registry...
February 14, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/28195581/cultivation-and-characterization-of-candidatus-nitrosocosmicus-exaquare-an-ammonia-oxidizing-archaeon-from-a-municipal-wastewater-treatment-system
#17
Laura A Sauder, Mads Albertsen, Katja Engel, Jasmin Schwarz, Per H Nielsen, Michael Wagner, Josh D Neufeld
Thaumarchaeota have been detected in several industrial and municipal wastewater treatment plants (WWTPs), despite the fact that ammonia-oxidizing archaea (AOA) are thought to be adapted to low ammonia environments. However, the activity, physiology and metabolism of WWTP-associated AOA remain poorly understood. We report the cultivation and complete genome sequence of Candidatus Nitrosocosmicus exaquare, a novel AOA representative from a municipal WWTP in Guelph, Ontario (Canada). In enrichment culture, Ca...
February 14, 2017: ISME Journal
https://www.readbyqxmd.com/read/28194147/freshwater-recirculating-aquaculture-system-operations-drive-biofilter-bacterial-community-shifts-around-a-stable-nitrifying-consortium-of-ammonia-oxidizing-archaea-and-comammox-nitrospira
#18
Ryan P Bartelme, Sandra L McLellan, Ryan J Newton
Recirculating aquaculture systems (RAS) are unique engineered ecosystems that minimize environmental perturbation by reducing nutrient pollution discharge. RAS typically employ a biofilter to control ammonia levels produced as a byproduct of fish protein catabolism. Nitrosomonas (ammonia-oxidizing), Nitrospira, and Nitrobacter (nitrite-oxidizing) species are thought to be the primary nitrifiers present in RAS biofilters. We explored this assertion by characterizing the biofilter bacterial and archaeal community of a commercial scale freshwater RAS that has been in operation for >15 years...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28161045/unraveling-the-active-microbial-populations-involved-in-nitrogen-utilization-in-a-vertical-subsurface-flow-constructed-wetland-treating-urban-wastewater
#19
Catiane Pelissari, Miriam Guivernau, Marc Viñas, Samara Silva de Souza, Joan García, Pablo Heleno Sezerino, Cristina Ávila
The dynamics of the active microbial populations involved in nitrogen transformation in a vertical subsurface flow constructed wetland (VF) treating urban wastewater was assessed. The wetland (1.5m(2)) operated under average loads of 130gCODm(-2)d(-1) and 17gTNm(-2)d(-1) in Period I, and 80gCODm(-2)d(-1) and 19gTNm(-2)d(-1) in Period II. The hydraulic loading rate (HLR) was 375mmd(-1) and C/N ratio was 2 in both periods. Samples for microbial characterization were collected from the filter medium (top and bottom layers) of the wetland, water influent and effluent at the end of Periods I (Jun-Oct) and II (Nov-Jan)...
April 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28151030/toxicity-of-tio2-nanoparticles-on-soil-nitrification-at-environmentally-relevant-concentrations-lack-of-classical-dose-response-relationships
#20
Marie Simonin, Jean M F Martins, Xavier Le Roux, Gaëlle Uzu, Aude Calas, Agnès Richaume
Titanium-dioxide nanoparticles (TiO2-NPs) are increasingly released in agricultural soils through, e.g. biosolids, irrigation or nanoagrochemicals. Soils are submitted to a wide range of concentrations of TiO2-NPs depending on the type of exposure. However, most studies have assessed the effects of unrealistically high concentrations, and the dose-response relationships are not well characterized for soil microbial communities. Here, using soil microcosms, we assessed the impact of TiO2-NPs at concentrations ranging from 0...
March 2017: Nanotoxicology
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