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https://www.readbyqxmd.com/read/28329240/effect-of-breathing-oxygen-enriched-air-on-exercise-performance-in-patients-with-precapillary-pulmonary-hypertension-randomized-sham-controlled-cross-over-trial
#1
Silvia Ulrich, Elisabeth D Hasler, Stéphanie Saxer, Michael Furian, Séverine Müller-Mottet, Stephan Keusch, Konrad E Bloch
Aims: The purpose of the current trial was to test the hypothesis that breathing oxygen-enriched air increases exercise performance of patients with pulmonary arterial or chronic thrombo-embolic pulmonary hypertension (PAH/CTEPH) and to investigate involved mechanisms. Methods and results: Twenty-two patients with PAH/CTEPH, eight women, means ± SD 61 ± 14 years, resting mPAP 35 ± 9mmHg, PaO2 ambient air >7.3 kPa, underwent four bicycle ergospirometries to exhaustion on different days, while breathing oxygen-enriched (FiO2 0...
March 11, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28327351/parkinson-s-patients-can-rely-on-perspective-cues-to-perceive-3d-space
#2
Alexandra Séverac Cauquil, Fabienne Ory-Magne, Vincent Jardiné, Monique Galitzky, Maxime Rosito, Christine Brefel-Courbon, Simona Celebrini
3D perception, which is necessary for an optimal navigation in our environment, relies on 2 complementary kinds of cues; binocular cues allowing precise depth localization near the point of visual interest and monocular ones that are necessary for correct global perception of visual space. Recent studies described deficient binocular 3D vision in PD patients; here we tested 3D vision in PD patients when based on monocular cues (m3D). Sixteen PD patients and 16 controls had to categorize visual stimuli as perceived in 2D (flat) or 3D (with depth)...
March 18, 2017: Brain Research
https://www.readbyqxmd.com/read/28325103/fabrication-and-characterization-of-dendrimer-functionalized-nano-hydroxyapatite-and-its-application-in-dentin-tubule-occlusion
#3
Xuandong Lin, Fangfang Xie, Xueling Ma, Yuhong Hao, Hejia Qin, Jindong Long
The occlusion of dentinal tubules is an effective method to alleviate the symptoms of dentin hypersensitivity. In this paper, we successfully modified nano-hydroxyapatite (n-HAP)with carboxyl-terminated polyamidoamine dendrimers by an aqueous-based chemical method and verified by fourier transform infrared spectroscopy (FTIR) and transmission electron microscope(TEM). Then the demineralization dentin discs were randomly divided into 4 groups, corresponding to subsequent brushing experiments: deionized water and kept in artificial saliva (AS), dendrimer-functionalized n-HAP and stored in AS, n-HAP and saved in AS, dendrimer-functionalized n-HAP and stored in deionized water...
March 22, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28323828/liquid-handling-lego-robots-and-experiments-for-stem-education-and-research
#4
Lukas C Gerber, Agnes Calasanz-Kaiser, Luke Hyman, Kateryna Voitiuk, Uday Patil, Ingmar H Riedel-Kruse
Liquid-handling robots have many applications for biotechnology and the life sciences, with increasing impact on everyday life. While playful robotics such as Lego Mindstorms significantly support education initiatives in mechatronics and programming, equivalent connections to the life sciences do not currently exist. To close this gap, we developed Lego-based pipetting robots that reliably handle liquid volumes from 1 ml down to the sub-μl range and that operate on standard laboratory plasticware, such as cuvettes and multiwell plates...
March 2017: PLoS Biology
https://www.readbyqxmd.com/read/28323684/shifting-spatial-neglect-with-repeated-line-bisections-possible-role-of-lateralized-attentional-fatigue
#5
Mary Woods, John B Williamson, Keith D White, Charles G Maitland, Kenneth M Heilman
BACKGROUND AND OBJECTIVE: Many patients who have signs of neglect immediately after a right hemisphere stroke remain disabled even when they improve on tests of neglect. Few patients are tested for attentional persistence and fatigue despite their importance in many instrumental activities. To investigate whether stimulus repetition might alter the allocation of attention, we repeatedly tested a patient 16 weeks after she developed hemispatial neglect from a right hemisphere stroke. METHODS: During each of three testing sessions given in 1 day, we asked the patient to bisect 90 lines of two lengths, presented in 30-trial blocks in three locations: left, center, and right of her midsagittal plane, partially counterbalanced across sessions...
March 2017: Cognitive and Behavioral Neurology: Official Journal of the Society for Behavioral and Cognitive Neurology
https://www.readbyqxmd.com/read/28323456/stratosphere-conditions-inactivate-bacterial-endospores-from-a-mars-spacecraft-assembly-facility
#6
Christina L Khodadad, Gregory M Wong, Leandro M James, Prital J Thakrar, Michael A Lane, John A Catechis, David J Smith
Every spacecraft sent to Mars is allowed to land viable microbial bioburden, including hardy endospore-forming bacteria resistant to environmental extremes. Earth's stratosphere is severely cold, dry, irradiated, and oligotrophic; it can be used as a stand-in location for predicting how stowaway microbes might respond to the martian surface. We launched E-MIST, a high-altitude NASA balloon payload on 10 October 2015 carrying known quantities of viable Bacillus pumilus SAFR-032 (4.07 × 10(7) spores per sample), a radiation-tolerant strain collected from a spacecraft assembly facility...
March 21, 2017: Astrobiology
https://www.readbyqxmd.com/read/28323277/an-alternative-polysaccharide-uptake-mechanism-of-marine-bacteria
#7
Greta Reintjes, Carol Arnosti, Bernhard M Fuchs, Rudolf Amann
Heterotrophic microbial communities process much of the carbon fixed by phytoplankton in the ocean, thus having a critical role in the global carbon cycle. A major fraction of the phytoplankton-derived substrates are high-molecular-weight (HMW) polysaccharides. For bacterial uptake, these substrates must initially be hydrolysed to smaller sizes by extracellular enzymes. We investigated polysaccharide hydrolysis by microbial communities during a transect of the Atlantic Ocean, and serendipitously discovered-using super-resolution structured illumination microscopy-that up to 26% of total cells showed uptake of fluorescently labelled polysaccharides (FLA-PS)...
March 21, 2017: ISME Journal
https://www.readbyqxmd.com/read/28321961/aging-affects-spatial-reconstruction-more-than-spatial-pattern-separation-performance-even-after-extended-practice
#8
Rachel Clark, Asli C Tahan, Patrick D Watson, Joan Severson, Neal J Cohen, Michelle Voss
Although the hippocampus experiences age-related anatomical and functional deterioration, the effects of aging vary across hippocampal-dependent cognitive processes. In particular, whether or not the hippocampus is known to be required for a spatial memory process is not an accurate predictor on its own of whether aging will affect performance. Therefore, the primary objective of this study was to compare the effects of healthy aging on a test of spatial pattern separation and a test of spatial relational processing, which are two aspects of spatial memory that uniquely emphasize the use of multiple hippocampal-dependent processes...
March 21, 2017: Hippocampus
https://www.readbyqxmd.com/read/28320908/reaction-and-relaxation-at-surface-hotspots-using-molecular-dynamics-and-the-energy-grained-master-equation-to-describe-diamond-etching
#9
David R Glowacki, W J Rodgers, Robin Shannon, Struan H Robertson, Jeremy N Harvey
The extent to which vibrational energy transfer dynamics can impact reaction outcomes beyond the gas phase remains an active research question. Molecular dynamics (MD) simulations are the method of choice for investigating such questions; however, they can be extremely expensive, and therefore it is worth developing cheaper models that are capable of furnishing reasonable results. This paper has two primary aims. First, we investigate the competition between energy relaxation and reaction at 'hotspots' that form on the surface of diamond during the chemical vapour deposition process...
April 28, 2017: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/28320847/multivoxel-pattern-analysis-reveals-3d-place-information-in-the-human-hippocampus
#10
Misun Kim, Kate J Jeffery, Eleanor A Maguire
The spatial world is three-dimensional (3D), and humans and other animals move both horizontally and vertically within it. Extant neuroscientific studies have typically investigated spatial navigation on a horizontal two-dimensional plane, leaving much unknown about how 3D spatial information is represented in the brain. Specifically, horizontal and vertical information may be encoded in the same or different neural structures with equal or unequal sensitivity. Here, we investigated these possibilities using functional MRI (fMRI) while participants were passively moved within a 3D lattice structure as if riding a rollercoaster...
March 20, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28319570/microcystic-elongated-and-fragmented-pattern-invasion-in-ovarian-endometrioid-carcinoma-immunohistochemical-profile-and-prognostic-implications
#11
Allison Goldberg, Lauren Hand, Dan DeCotiis, Norman Rosenblum, Joanna Chan
Microcystic, elongated, and fragmented (MELF) pattern invasion is a poor prognostic indicator in uterine endometrioid carcinoma, but its existence, biology, and prognostic value have not been described in ovarian endometrioid carcinoma. We evaluated cases of ovarian endometrioid carcinoma without synchronous uterine endometrioid carcinoma for MELF and other histologic features. To evaluate tumor biology, we assessed an immunohistochemical profile, including MLH1, PMS2, MSH2, MSH6, β-catenin, e-cadherin, CK19, and cyclin D1...
March 17, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/28318800/autoimmune-disease-development-based-on-possible-mislocalization-of-intracellular-and-extracellular-proteins
#12
Borros Arneth
BACKGROUND: T helper cells can differentiate into several subsets of T lymphocytes, including Th1, Th2, and regulatory T (Treg) cells. As a result of this ability to differentiate, the corresponding T cell receptor (TCR) spectra display considerable cellular plasticity and interchangeability. In contrast, T lymphocyte differentiation and separation into CD4(+) and/or CD8(+) T cell lines creates stable populations over a person's lifetime, which abrogates the plasticity and interchange between these cell types and their corresponding TCR spectra but results in considerable stability regarding the corresponding TCR sequences and spectra...
February 27, 2017: Immunobiology
https://www.readbyqxmd.com/read/28318312/evidence-favoring-a-secular-reduction-in-mandibular-leeway-space
#13
Tyler R Allen, Terry M Trojan, Edward F Harris
OBJECTIVE: Researchers have documented secular trends in tooth size among recent generations. This study was a test for a change in mandibular leeway space. MATERIALS AND METHODS: Dental casts from participants in the Denver Growth Study (23 boys, 22 girls; born in the 1930s) were compared with casts from a contemporary series of orthodontic patients (23 boys, 22 girls; born in the 1990s). All were phenotypically normal, healthy American whites. RESULTS: Analysis of variance (accounting for sex) showed that the cumulative mandibular primary canine plus first and second primary molar size (c + m1 + m2) was slightly larger in the recent cohort (23...
March 20, 2017: Angle Orthodontist
https://www.readbyqxmd.com/read/28316586/spatial-and-temporal-high-processing-of-visual-and-auditory-stimuli-in-cervical-dystonia
#14
Gaetana Chillemi, Alessandro Calamuneri, Francesca Morgante, Carmen Terranova, Vincenzo Rizzo, Paolo Girlanda, Maria Felice Ghilardi, Angelo Quartarone
OBJECTIVE: Investigation of spatial and temporal cognitive processing in idiopathic cervical dystonia (CD) by means of specific tasks based on perception in time and space domains of visual and auditory stimuli. BACKGROUND: Previous psychophysiological studies have investigated temporal and spatial characteristics of neural processing of sensory stimuli (mainly somatosensorial and visual), whereas the definition of such processing at higher cognitive level has not been sufficiently addressed...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28315969/time-takes-space-selective-effects-of-multitasking-on-concurrent-spatial-processing
#15
Timo Mäntylä, Valentina Coni, Veit Kubik, Ivo Todorov, Fabio Del Missier
Many everyday activities require coordination and monitoring of complex relations of future goals and deadlines. Cognitive offloading may provide an efficient strategy for reducing control demands by representing future goals and deadlines as a pattern of spatial relations. We tested the hypothesis that multiple-task monitoring involves time-to-space transformational processes, and that these spatial effects are selective with greater demands on coordinate (metric) than categorical (nonmetric) spatial relation processing...
March 18, 2017: Cognitive Processing
https://www.readbyqxmd.com/read/28315820/evaluating-differential-nuclear-dna-yield-rates-and-osteocyte-numbers-among-human-bone-tissue-types-a-synchrotron-radiation-micro-ct-approach
#16
Janna M Andronowski, Amy Z Mundorff, Isaac V Pratt, Jon M Davoren, David M L Cooper
Molecular human identification has conventionally focused on DNA sampling from dense, weight-bearing cortical bone tissue, typically from femora or tibiae. A comparison of skeletal elements from three contemporary individuals demonstrated that elements with high quantities of cancellous bone yielded nuclear DNA at the highest rates, suggesting that preferentially sampling cortical bone may be suboptimal (Mundorff & Davoren, 2014). Despite these findings, the reason for the differential DNA yields between cortical and cancellous bone tissues remains unknown...
March 6, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28315245/multidimensional-analysis-of-protease-substrates-and-their-cellular-origins-in-mixed-secretomes-from-multiple-cell-types
#17
Pascal Schlage, Ulrich Auf dem Keller
Although extracellular proteases are confronted with substrate proteins expressed by multiple cell types in vivo, in most protease substrate discovery approaches, the test protease is exposed to a test proteome (secretome) derived only from a single cell type. This limits the potential substrate space and prohibits the formation of protein complexes constituted of components derived from multiple cellular origins. Mixing of secretomes collected from multiple cell types addresses this issue, but information on the cellular origin of a substrate protein is lost...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28314254/synchronous-endometrial-and-ovarian-cancer-in-young-women-case-report-and-review-of-the-literature
#18
REVIEW
Askin Dogan, Beate Schultheis, Günther A Rezniczek, Ziad Hilal, Cem Cetin, Günther Häusler, Clemens B Tempfer
BACKGROUND: Young women with endometrial cancer (EC) have an increased risk of synchronous ovarian cancer. The prognosis of women with synchronous endometrial and ovarian cancer (SEOC) is good. A high proportion of affected women have hereditary non-polyposis colon cancer syndrome (HNPCC). CASE PRESENTATION: We present the case of a 45-year-old woman with histologically proven endometrioid adenocarcinoma of the endometrium (pT1B, G2, R0 without lymphovascular space invasion)...
March 2017: Anticancer Research
https://www.readbyqxmd.com/read/28306518/biochemical-testing-in-a-laboratory-tent-and-semi-intensive-care-of-ebola-patients-on-site-in-a-remote-part-of-guinea-a-paradigm-shift-based-on-a-bleach-sensitive-point-of-care-device
#19
Leonid M Irenge, Jean-Michel Dindart, Jean-Luc Gala
BACKGROUND: During the West Africa Ebola virus disease (EVD) outbreak, a Belgian laboratory was deployed for supporting the Ebola treatment unit (ETU) of N'Zerekore, Guinea. Besides diagnosis of EVD and malaria, biochemical parameters were tested and used to guide supportive treatment of EVD. METHODS: To preserve analytes stability, lithium-heparin blood samples were analyzed using the i-STAT® point-of-care testing (POCT) handheld device without the viral inactivation step...
March 15, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28306488/spinal-neurenteric-cyst-in-a-dog
#20
Daniela S Alder, Anna Oevermann, Stephan A Pfister, Frank Steffen
CASE DESCRIPTION An 11-year-old English Cocker Spaniel was evaluated because of chronic progressive ataxia of the hind limbs. CLINICAL FINDINGS The dog had no history of previous illness, and findings of physical examination and laboratory tests were unremarkable. Neurologic examination revealed that the dog was ambulatory with severe ataxia of the hind limbs. Proprioception was decreased in the right and left hind limbs (right affected more than left), and spinal reflexes were bilaterally unremarkable. Moderate signs of pain were detected during palpation of the lumbar portion of the vertebral column...
April 1, 2017: Journal of the American Veterinary Medical Association
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