keyword
https://read.qxmd.com/read/38513646/flex-genetically-encodable-enzymatic-fluorescence-signal-amplification-using-engineered-peroxidase
#1
JOURNAL ARTICLE
Nirmali Sharma, Minkyo Jung, Pratyush Kumar Mishra, Ji Young Mun, Hyun-Woo Rhee
Fluorescent tagging of biomolecules enables their sensitive detection during separation and determining their subcellular location. In this context, peroxidase-based reactions are actively utilized for signal amplification. To harness this potential, we developed a genetically encodable enzymatic fluorescence signal amplification method using APEX (FLEX). We synthesized a fluorescent probe, Jenfluor triazole (JFT1), which effectively amplifies and restricts fluorescence signals under fixed conditions, enabling fluorescence-based detection of subcellularly localized electron-rich metabolites...
March 8, 2024: Cell Chemical Biology
https://read.qxmd.com/read/38510318/soft-tissue-stabilization-of-the-hinge-position-for-lateral-closing-wedge-distal-femoral-osteotomy-an-anatomic-study
#2
JOURNAL ARTICLE
Taiga Oda, Akira Maeyama, Tetsuro Ishimatsu, Katsuro Tachibana, Kengo Yoshimitsu, Takuaki Yamamoto
BACKGROUND: Soft tissue plays an important role in stabilizing the hinge point for osteotomy around the knee. However, insufficient data are available on the anatomic features of the soft tissue around the hinge position for lateral closing-wedge distal femoral osteotomy (LCWDFO). PURPOSE: To (1) anatomically analyze the soft tissue around the hinge position for LCWDFO, (2) histologically analyze the soft tissue based on the anatomic analysis results, and (3) radiologically define the appropriate hinge point to prevent unstable hinge fracture based on the results of the anatomic and histological analyses...
March 2024: Orthopaedic Journal of Sports Medicine
https://read.qxmd.com/read/38481723/comparative-evaluation-of-digital-radiography-electronic-apex-locator-and-simultaneous-working-length-determination-on-postoperative-pain-after-root-canal-treatment-a-randomized-clinical-trial
#3
JOURNAL ARTICLE
Boris Saha, Sharique Alam, Daiasharailang Lyngdoh, Surendra Kumar Mishra
PURPOSE: The study aimed to compare postoperative pain after root canal preparation using three different methods of working length determination. MATERIALS AND METHODS: 60 patients diagnosed with symptomatic irreversible pulpitis were randomly divided into three groups based on the method of working length (WL) determination. Group 1: digital radiograph (DRG), Group 2: electronic apex locator (EAL), Group 3: the simultaneous working length control (SLC) method using an endomotor with an integrated apex locator...
January 5, 2024: European Oral Research
https://read.qxmd.com/read/38425325/comparison-of-the-accuracy-of-apex-locator-digital-radiography-and-cone-beam-computed-tomography-in-root-canal-working-length-determination-in-teeth-with-external-root-resorption-an-in-vitro-study
#4
JOURNAL ARTICLE
Seyed Mohsen Hasheminia, Sanaz Jahadi, Farida Ghazanfari Moghaddam, Shervin Bagherieh
BACKGROUND: The aim of this study was to compare the accuracy of apex locator, digital periapical radiography, and cone-beam computed tomography (CBCT) for determining the root canal working length (WL) in teeth with external root resorption (ERR). MATERIALS AND METHODS: In this in vitro study, the sample consisted of 54 extracted permanent single-rooted human teeth. ERRs were performed at the 3 mm apical root using 65% of nitric acid for 24 h. After determining the actual WL by K-file #10 (gold standard) with the visualization method, the teeth were mounted in alginate and the WL of each tooth was determined using the electronic apex locator (EAL) equipped with a K-file #15...
2024: Dental Research Journal
https://read.qxmd.com/read/38378499/forced-eruption-in-impacted-teeth-analysis-of-failed-cases-and-outcome-of-re-operation
#5
JOURNAL ARTICLE
Jaeyeon Kim, Seoyeon Jung, Kee-Joon Lee, Hyung-Seog Yu, Wonse Park
BACKGROUND: Forced eruption of an impacted tooth usually requires surgical and orthodontic interventions to successfully bring the tooth into the dental arch. The clinical time required for a forced eruption is difficult to predict before treatment begins and success rates are affected by several factors before and after an eruption. This study was conducted to identify factors that affect the success of forced eruption, the duration of orthodontic treatment of impacted teeth, and the reasons for re-operation and forced eruption failure in a various teeth and cases...
February 20, 2024: BMC Oral Health
https://read.qxmd.com/read/38319145/impact-of-lead-position-on-tricuspid-regurgitation-ventricular-function-and-heart-failure-exacerbation-and-mortality-after-cardiac-implantable-electronic-device-implantation-preliminary-results-from-the-pace-rvtr-registry
#6
JOURNAL ARTICLE
Karolina Chodór-Rozwadowska, Magdalena Sawicka, Stanisław Morawski, Zbigniew Kalarus, Tomasz Kukulski
BACKGROUND: The most frequent mechanism of lead-related tricuspid regurgitation (LRTR), which occurs in 7.2% to 44.7% of patients implanted with a cardiac implantable electronic device (CIED), is leaflet impingement or the restriction of its movement by a ventricular lead. It is unclear if the position of the lead tip - in the right ventricular apex (RVA) or other location (non-RVA) - has any influence on the development of LRTR. The study aimed to determine the impact of the CIED lead tip position on the development or progression of tricuspid regurgitation (TR) and its potential impact on heart failure exacerbation and mortality...
2024: Kardiologia Polska
https://read.qxmd.com/read/38169076/identification-of-fourth-instar-larvae-of-aedes-albopictus-skuse-diptera-culicidae-employing-scanning-electron-microscopic-tool
#7
JOURNAL ARTICLE
Abeer S Yamany, Rewaida Abdel-Gaber
Aedes albopictus is a vector of numerous viruses in tropical and subtropical regions worldwide, including encephalitis and dengue fever. Although the identification keys depend exclusively on the characteristics of the fourth larval instar, the studies were carried out using scanning electron microscopy (SEM) on the morphology of Ae. albopictus are still insufficient, and more research is required. Therefore, the purpose of this study was to present a comprehensive description of Ae. albopictus mosquitoes based on the morphological features of their fourth-instar larvae to distinguish them from other mosquito species...
January 3, 2024: Microscopy Research and Technique
https://read.qxmd.com/read/38152904/influence-of-embedding-medium-on-ipex-electronic-apex-locator-accuracy-an-ex-vivo-comparative-study-using-alginate-saline-and-gelatin-models
#8
JOURNAL ARTICLE
Amna Y Siddiqui
AIM: Accurate working length determination during root canal treatment is essential for achieving successful outcomes. This study aimed to evaluate the impact of embedding medium on the accuracy of iPex electronic apex locator (EAL). MATERIALS AND METHODS: Sixty-one extracted single-rooted teeth were decoronated and coronally flared with Gates-Glidden burs. Actual canal length (ACL) was obtained by introducing a size 8 K-file until its tip reached the most coronal border of the apical foramen...
October 1, 2023: Journal of Contemporary Dental Practice
https://read.qxmd.com/read/38123367/antillean-manatee-trichechus-manatus-manatus-linnaeus-1758-tongue-morphology-and-adaptive-herbivorous-implications
#9
JOURNAL ARTICLE
Lucas Inácio Dos Santos Melo, Radan Elvis Matias de Oliveira, Ana Caroline Freitas Caetano de Sousa, Rysónely Maclay de Oliveira, Mariana Almeida Lima, Ana Bernadete Lima Fragoso, Flávio José de Lima Silva, Fernanda Loffler Niemeyer Attademo, Fábia de Oliveira Luna, Alexsandra Fernandes Pereira, Moacir Franco de Oliveira
Morphological study of the tongue is an interesting way of understanding evolutionary processes associated with feeding habits. Therefore, the aim of the present study was to describe the tongue morphology of the Antillean manatee and to understand possible morphological relationships with its way of capturing food. Macroscopic dissections and light and scanning electron microscopy analyses of seven manatee tongues were performed. The tongue in Antillean manatees is a muscular and robust organ, divided into apex, body, and root...
December 20, 2023: Microscopy and Microanalysis
https://read.qxmd.com/read/37997246/evaluation-of-accuracy-of-an-electronic-apex-locator-in-presence-of-sodium-hypochlorite-in-primary-teeth-with-and-without-resorption
#10
JOURNAL ARTICLE
Gamze Topçuoğlu, Kevser Kolçakoğlu
This study compared the accuracy of Root ZX mini apex locator in presence of sodium hypochlorite (NaOCl) in primary molars with and without apical resorption. Sixty-four extracted primary lower molar teeth with 32 root resorption and 32 without resorption were selected. To determine the actual working length (AWL), a K-file was inserted into the root canal until the tip of the file was visible at the major foramen or the resolution level. It was then withdrawn 1 mm. This value was recorded as AWL. The teeth were then divided subgroups (with/without NaOCl)...
November 2023: Journal of Clinical Pediatric Dentistry
https://read.qxmd.com/read/37965585/the-effect-of-coronal-pre-flaring-and-type-of-root-canal-irrigation-on-working-length-accuracy-using-electronic-apex-locators
#11
JOURNAL ARTICLE
Shimaa Rifaat, Abdullah Aljami, Turki Alshehri, Shahad T Alameer, Alhanoof Aldossary, Wejdan Almutairi, Mulham N Almaliki, Faraz A Farooqi, Noha Taymour
Background: Successful root canal treatment is influenced by the apical extent of root canal preparation and the eventual root canal filling. Achieving the full working length until the apical constriction, which is usually 0.5 - 1 mm shorter than the anatomical apex, is crucial. Electronic apex locators were used to detect the working length more accurately. There are six generations of electronic apex locators in the market. The selection of the appropriate irrigation with each apex locator for accurate working length determination is not fully investigated...
2023: F1000Research
https://read.qxmd.com/read/37819467/can-ultrasonography-be-used-to-determine-working-length-in-endodontic-treatment-an-in-vitro-study
#12
JOURNAL ARTICLE
İrem Eren, Öznur Sarıyılmaz, Hakan Eren, Evren Sarıyılmaz, Gülşah Uslu
This study was conducted to evaluate efficacy of ultrasonography (USG) in determining working length in in-vitro conditions. Twenty five access cavities of maxillary incisor teeth were opened and actual working lengths (AWL) were measured with dental operating microscope. The working length were then measured with an electronic apex locator and USG. USG and apex locator measurements were statistically analyzed using one sample t-test and compared with AWL. The mean AWL measurment was 20.68 mm. USG measured the working length slightly longer (21...
October 11, 2023: Odontology
https://read.qxmd.com/read/37800848/global-survey-of-endodontic-practice-and-adoption-of-newer-technologies
#13
JOURNAL ARTICLE
Monique Charlene Cheung, Ove Andreas Peters, Peter Parashos
AIM: To investigate current endodontic practices, adoption of technologies and continuing education attendance within specialist endodontic practice globally and to identify geographic trends. METHODOLOGY: A web-based survey of endodontic association members in Australia, Britain, Canada, Italy, New Zealand and the USA on routine treatment preferences, armamentarium and education attendance was conducted. Chi-squared, independent sample t-tests, Cochran's Q test and McNemar's test were performed...
October 6, 2023: International Endodontic Journal
https://read.qxmd.com/read/37739340/in-vitro-comparison-of-the-accuracy-of-cone-beam-computed-tomography-scanning-and-electronic-apex-locators-in-detection-of-simulated-root-perforations-in-different-localizations
#14
JOURNAL ARTICLE
Hatice Harorlı, Simay Koç, Alper Kuştarcı
AIM: This study aimed to compare the accuracy of cone-beam computed tomography (CBCT) scanning and two different electronic apex locators (EALs) in the detection of simulated root canal perforations in different localizations. MATERIALS AND METHOD: Eighty human mandibular premolars were prepared and divided into two groups according to the localization of the perforation area, having a 1 mm diameter. The distance between the occlusal edge and the beginning of the perforation was measured under a stereomicroscope (actual working length=AWL), by using two electronic apex locators (Propex II and Dentaport ZX) (electronic working length=EWL) and CBCT images (CWL)...
September 20, 2023: Journal of Endodontics
https://read.qxmd.com/read/37693955/-in-vivo-study-to-evaluate-the-effect-of-instrument-size-on-the-accuracy-of-three-different-apex-locators-when-various-irrigation-solutions-are-used-in-vital-and-non-vital-teeth
#15
JOURNAL ARTICLE
Akhilesh Vajpayee, Prachi D Khandare, Smita D Dutta, Seema Marathe, Prashant Viragi, Rahul Maria
INTRODUCTION: Root canal success requires working length (WL) optimization. The current study examined how different apex locators on vital and non-vital teeth responded to instrument size. MATERIALS AND METHODS: Three hundred patients (180 men and 120 women) receiving root canal therapy at the department of Conservative Dentistry and Endodontics were evaluated. Therefore, we determined that 200 of the patients' teeth (100 vital and 100 non-vital teeth from the upper and lower jaws' first molars) qualified for inclusion in our study...
July 2023: Journal of Pharmacy & Bioallied Sciences
https://read.qxmd.com/read/37671079/accuracy-of-root-zx-electronic-apex-locator-in-relation-to-two-different-employment-protocols-an-in-vitro-study
#16
JOURNAL ARTICLE
Osama S Alothmani, Amna Y Siddiqui
Objective The aim of this study is to determine the apical level of the root canal, whether it is the apical foramen or a level coronal to it, that Root ZX (J. Morita Co., Kyoto, Japan) targets and to identify its employment protocol that provides better accuracy. Methods Actual lengths (ALs) of 75 extracted single-rooted teeth were obtained by inserting a K-file size 8 until its tip was in level with the most coronal border of the apical foramen. Reference length (RL) was calculated by deducting 0.5 mm from AL...
September 2023: Curēus
https://read.qxmd.com/read/37644294/evaluation-of-changes-in-root-canal-length-and-accuracy-of-the-electronic-apex-locator-during-different-stages-of-endodontic-treatment-and-retreatment
#17
JOURNAL ARTICLE
Ihan Vitor Cardoso, Matheus Pompeo Caldas Silveira, Filipe Colombo Vitali, Lucila Piasecki, Lucas da Fonseca Roberti Garcia, Eduardo Antunes Bortoluzzi, Cleonice Silveira Teixeira
This study evaluated changes in the root canal length (RCL) and the accuracy of the electronic apex locator (EAL) during the different stages of endodontic treatment and retreatment. Fifty-six mesial root canals of mandibular molars were selected. The actual root canal length (AL) of the canals was obtained by inserting a size 15 hand file up to the apical foramen, under magnification. The electronic lengths were obtained at the "APEX" mark of Root ZX II, using an alginate model. Both measurements were performed at three different stages of the initial root canal treatment-unflared, flared, and concluded-and at two stages of retreatment, after achieving patency and repreparation...
August 29, 2023: Odontology
https://read.qxmd.com/read/37635899/the-effect-of-patency-files-on-apical-canal-anatomy-using-sem-imaging
#18
JOURNAL ARTICLE
Michael S Cavender, Christopher Waters
INTRODUCTION: There are many reasons to maintain apical patency during routine endodontic treatment. Thousands of canals are treated utilizing a patency file every year all around the world. The effect patency has on the apical anatomy of the root has been controversial for generations. OBJECTIVE: This ex vivo descriptive study was created to show the effect patency files actually have on the apical root canal anatomy using visually detailed SEM images supported by dental radiographs...
2023: International Journal of Dentistry
https://read.qxmd.com/read/37422252/prevalence-of-teaching-apical-patency-and-various-instrumentation-and-obturation-techniques-in-united-states-dental-schools-two-decades-later
#19
JOURNAL ARTICLE
Tiffany Do, Maobin Yang, Jong Ryul Kim
INTRODUCTION: Maintaining apical patency has been advocated to minimize canal transportation, ledge formation, and loss of working length, despite increasing debris extrusion. A previous study in 1997 by Cailleteau and Mullaney reported 50% of United States dental schools taught patency. This present study aimed to evaluate recent trends in endodontic education in United States dental schools, in relation to the prevalence of maintaining apical patency and examining the primary methods of working length determination, instrumentation, obturation, and temporization...
July 7, 2023: Journal of Endodontics
https://read.qxmd.com/read/37398858/accuracy-of-the-electronic-apex-locator-tactile-and-radiographic-methods-in-working-length-determination
#20
JOURNAL ARTICLE
Frank Osei-Bonsu, Patrick Caldicock Ampofo, Ebenezer Anno Nyako, Sandra Ama Hewlett, Victoria Afi Buckman, Akua Boakyewa Konadu, Paa-Kwesi Blankson, Tom Ndanu
BACKGROUND: Determination of working length (WL) is necessary for the successful outcome of root canal treatment (RCT). Common methods in WL determination include tactile, radiographic, and electronic apex locators (EAL). AIM: The aim of this study was to compare three methods of WL determination to the actual visualization of the apical constriction (AC). MATERIALS AND METHODS: Consecutive patients with indications for extraction of single-rooted single canal teeth at the University of Ghana Dental School clinic were randomly assigned to three groups...
2023: Journal of Conservative Dentistry: JCD
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