keyword
MENU ▼
Read by QxMD icon Read
search

Extracellular RNA

keyword
https://www.readbyqxmd.com/read/29677479/rig-i-and-other-rna-sensors-in-antiviral-immunity
#1
Kwan T Chow, Michael Gale, Yueh-Ming Loo
Pattern recognition receptors (PRRs) survey intra- and extracellular spaces for pathogen-associated molecular patterns (PAMPs) within microbial products of infection. Recognition and binding to cognate PAMP ligand by specific PRRs initiates signaling cascades that culminate in a coordinated intracellular innate immune response designed to control infection. In particular, our immune system has evolved specialized PRRs to discriminate viral nucleic acid from host. These are critical sensors of viral RNA to trigger innate immunity in the vertebrate host...
April 26, 2018: Annual Review of Immunology
https://www.readbyqxmd.com/read/29676176/effect-of-mecp2-on-tgf-%C3%AE-1-induced-extracellular-matrix-production-in-nasal-polyp-derived-fibroblasts
#2
Jae-Min Shin, Ji-Young Um, Seoung-Ae Lee, Il-Ho Park, Soo-Hyung Lee, Heung-Man Lee
Purpose Methyl-CpG-binding protein 2 (MeCP2), known as a transcriptional regulator, has been suggested to play an important role in myofibroblast differentiation in the lung. The purpose of this study was to investigate the role of MeCP2 in transforming growth factor (TGF)- β1-induced myofibroblast differentiation and extracellular matrix (ECM) production in nasal polyp-derived fibroblasts (NPDFs). Methods To identify the expression of MeCP2 in nasal polyp tissues, immunohistochemistry staining and Western blot were performed...
January 1, 2018: American Journal of Rhinology & Allergy
https://www.readbyqxmd.com/read/29674954/bioinformatic-integration-of-molecular-networks-and-major-pathways-involved-in-mice-cochlear-and-vestibular-supporting-cells
#3
Teresa Requena, Alvaro Gallego-Martinez, Jose A Lopez-Escamez
Background : Cochlear and vestibular epithelial non-hair cells (ENHCs) are the supporting elements of the cellular architecture in the organ of Corti and the vestibular neuroepithelium in the inner ear. Intercellular and cell-extracellular matrix interactions are essential to prevent an abnormal ion redistribution leading to hearing and vestibular loss. The aim of this study is to define the main pathways and molecular networks in the mouse ENHCs. Methods : We retrieved microarray and RNA-seq datasets from mouse epithelial sensory and non-sensory cells from gEAR portal (http://umgear...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29673133/role-of-the-erk1-2-signaling-pathway-in-the-replication-of-jun%C3%A3-n-and-tacaribe-viruses
#4
Jesús E Brunetti, Sabrina Foscaldi, Verónica M Quintana, Luis A Scolaro, Nora López, Viviana Castilla
We have previously shown that the infection of cell cultures with the arenaviruses Junín (JUNV), Tacaribe (TCRV), and Pichindé promotes the phosphorylation of mitogen-activated protein kinases (MAPKs) extracellular signal-regulated kinases 1 and 2 (ERK1/2) and that this activation is required for the achievement of a productive infection. Here we examined the contribution of ERK1/2 in early steps of JUNV and TCRV multiplication. JUNV adsorption, internalization, and uncoating were not affected by treatment of cultured cells with U0126, an inhibitor of the ERK1/2 signaling pathway...
April 17, 2018: Viruses
https://www.readbyqxmd.com/read/29670130/a-novel-s100a8-a9-induced-fingerprint-of-mesenchymal-stem-cells-associated-with-enhanced-wound-healing
#5
Abhijit Basu, Saira Munir, Medanie A Mulaw, Karmveer Singh, Benedikt Herold, Diana Crisan, Anca Sindrilaru, Nicolai Treiber, Meinhard Wlaschek, Markus Huber-Lang, Florian Gebhard, Karin Scharffetter-Kochanek
We here investigated whether the unique capacity of mesenchymal stem cells (MSCs) to re-establish tissue homeostasis depends on their potential to sense danger associated molecular pattern (DAMP) and to mount an adaptive response in the interest of tissue repair. Unexpectedly, after injection of MSCs which had been pretreated with the calcium-binding DAMP protein S100A8/A9 into murine full-thickness wounds, we observed a significant acceleration of healing even exceeding that of non-treated MSCs. This correlates with a fundamental reprogramming of the transcriptome in S100A8/A9 treated MSCs as deduced from RNA-seq analysis and its validation...
April 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29669525/salivary-extracellular-vesicle-associated-mirnas-as-potential-biomarkers-in-oral-squamous-cell-carcinoma
#6
Chiara Gai, Francesco Camussi, Roberto Broccoletti, Alessio Gambino, Marco Cabras, Luca Molinaro, Stefano Carossa, Giovanni Camussi, Paolo G Arduino
BACKGROUND: Several studies in the past have investigated the expression of micro RNAs (miRNAs) in saliva as potential biomarkers. Since miRNAs associated with extracellular vesicles (EVs) are known to be protected from enzymatic degradation, we evaluated whether salivary EVs from patients with oral squamous cell carcinoma (OSCC) were enriched with specific subsets of miRNAs. METHODS: OSCC patients and controls were matched with regards to age, gender and risk factors...
April 18, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29668735/mus-52-disruption-and-metabolic-regulation-in-neurospora-crassa-transcriptional-responses-to-extracellular-phosphate-availability
#7
Maíra P Martins, Eriston V Gomes, Pablo R Sanches, Wellington R Pedersoli, Nilce M Martinez-Rossi, Antonio Rossi
Advances in the understanding of molecular systems depend on specific tools like the disruption of genes to produce strains with the desired characteristics. The disruption of any mutagen sensitive (mus) genes in the model fungus Neurospora crassa, i.e. mus-51, mus-52, or mus-53, orthologous to the human genes KU70, KU80, and LIG4, respectively, provides efficient tools for gene targeting. Accordingly, we used RNA-sequencing and reverse transcription-quantitative polymerase chain reaction amplification techniques to evaluate the effects of mus-52 deletion in N...
2018: PloS One
https://www.readbyqxmd.com/read/29666835/aptamers-and-apple-pies-a-mini-review-of-psma-aptamers-and-lessons-from-donald-s-coffey
#8
REVIEW
Shawn E Lupold
This mini-review article is part of a special issue dedicated to Donald S. Coffey, a pioneer translational research scientist, exemplary mentor, and leader in urologic and urologic oncology research. This article first briefly reflects on life and scientific lessons from Don Coffey. It then reviews the development of two prostate cancer targeting RNA aptamers, xPSM-A9 and xPSM-A10, through in vitro selection for aptamers that bind to the extracellular domain of the Prostate Specific Membrane Antigen (PSMA)...
2018: American Journal of Clinical and Experimental Urology
https://www.readbyqxmd.com/read/29666211/drivers-of-persistent-infection-pathogen-induced-extracellular-vesicles
#9
REVIEW
Michael J Cipriano, Stephen L Hajduk
Extracellular vesicles (EVs) are produced by invading pathogens and also by host cells in response to infection. The origin, composition, and function of EVs made during infection are diverse and provide effective vehicles for localized and broad dissimilation of effector molecules in the infected host. Extracellular pathogens use EVs to communicate with each other by sensing the host environment contributing to social motility, tissue tropism, and persistence of infection. Pathogen-derived EVs can also interact with host cells to influence the adhesive properties of host membranes and to alter immune recognition and response...
April 17, 2018: Essays in Biochemistry
https://www.readbyqxmd.com/read/29665744/expression-and-functions-of-formyl-peptide-receptor-1-in-drug-resistant-bladder-cancer
#10
Xue Jiang, Ting Lei, Man Zhang
OBJECTIVE: To explore the correlation of formyl peptide receptor 1 expression with drug resistance and the functions of formyl peptide receptor 1 in drug-resistant bladder cancer. METHODS: Expression of formyl peptide receptor 1 in T24 and T24/DDP cisplatin-resistant bladder cancer cell lines was tested by quantitative real-time Polymerase Chain Reaction and Western blotting. After incubation of T24/DDP with N-formyl-Met-Leu-Phe, the phosphor proteins were tested by Western blot analysis...
January 1, 2018: Technology in Cancer Research & Treatment
https://www.readbyqxmd.com/read/29659700/sex-steroids-drive-the-remodeling-of-oviductal-extracellular-matrix-in-cattle
#11
Angela Maria Gonella-Diaza, Fernando Silveira Mesquita, Everton Lopes, Kauê Ribeiro da Silva, Bruno Cogliati, Ricardo De Francisco Strefezzi, Mario Binelli
The extracellular matrix (ECM) is a group of molecules that offer structural and biochemical support to cells and interact with them to regulate their function. Also, growth factors (GFs) stored in the ECM can be locally released during the ECM remodeling process. Here, we hypothesize that the balance between ECM components and remodelers is regulated according to the ovarian steroid milieu to which the oviduct is exposed during the periovulatory period. Follicular growth was manipulated to generate cows that ovulated small follicles (SF-SCL; n = 20) or large follicles (LF-LCL; n = 21) and possess corresponding Estradiol (E2) and Progesterone (P4) plasmatic concentrations...
April 5, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29659599/profiling-extracellular-vesicle-release-by-the-filarial-nematode-brugia-malayi-reveals-sex-specific-differences-in-cargo-and-a-sensitivity-to-ivermectin
#12
Hiruni Harischandra, Wang Yuan, Hannah J Loghry, Mostafa Zamanian, Michael J Kimber
The filarial nematode Brugia malayi is an etiological agent of Lymphatic Filariasis. The capability of B. malayi and other parasitic nematodes to modulate host biology is recognized but the mechanisms by which such manipulation occurs are obscure. An emerging paradigm is the release of parasite-derived extracellular vesicles (EV) containing bioactive proteins and small RNA species that allow secretion of parasite effector molecules and their potential trafficking to host tissues. We have previously described EV release from the infectious L3 stage B...
April 16, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29657028/monosodium-urate-crystals-induced-icam-1-expression-and-cell-cell-adhesion-in-renal-mesangial-cells-implications-for-the-pathogenesis-of-gouty-nephropathy
#13
Shue-Fen Luo, Chia-Yin Chin, Ling-Jun Ho, Wen-Yi Tseng, Chang-Fu Kuo, Jenn-Haung Lai
BACKGROUND: Renal disease is prevalent in gouty patients and monosodium urate (MSU) crystal deposition in the kidney can be detected in some gouty nephropathy patients. MSU crystals can induce inflammatory events, we investigated the MSU-induced expression of intercellular adhesion molecule (ICAM)-1 on human renal mesangial cells (HRMCs) and the involved signal transduction mechanisms. METHODS: The HRMCs cell line was purchased from ScienCell Research Laboratories...
January 31, 2018: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29656370/role-of-exosomes-in-human-retroviral-mediated-disorders
#14
REVIEW
Monique Anderson, Fatah Kashanchi, Steven Jacobson
Retroviruses comprise an ancient and varied group of viruses with the unique ability to integrate DNA from an RNA transcript into the genome, a subset of which are able to integrate in humans. The timing of these integrations during human history has dictated whether these viruses have remained exogenous and given rise to various human diseases or have become inseparable from the host genome (endogenous retroviruses). Given the ability of retroviruses to integrate into the host and subsequently co-opt host cellular process for viral propagation, retroviruses have been shown to be closely associated with several cellular processes including exosome formation...
April 14, 2018: Journal of Neuroimmune Pharmacology: the Official Journal of the Society on NeuroImmune Pharmacology
https://www.readbyqxmd.com/read/29656147/liver-fibrosis-direct-antifibrotic-agents-and-targeted-therapies
#15
REVIEW
Detlef Schuppan, Muhammad Ashfaq-Khan, Ai Ting Yang, Yong Ook Kim
Liver fibrosis and in particular cirrhosis are the major causes of morbidity and mortality of patients with chronic liver disease. Their prevention or reversal have become major endpoints in clinical trials with novel liver specific drugs. Remarkable progress has been made with therapies that efficiently address the cause of the underlying liver disease, as in chronic hepatitis B and C. Highly effective antiviral therapy can prevent progression or even induce reversal in the majority of patients, but such treatment remains elusive for the majority of liver patients with advanced alcoholic or nonalcoholic steatohepatitis, genetic or autoimmune liver diseases...
April 12, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29653293/transcriptional-profiling-of-murine-osteoblast-differentiation-based-on-rna-seq-expression-analyses
#16
Layal Abo Khayal, Johannes Grünhagen, Ivo Provazník, Stefan Mundlos, Uwe Kornak, Peter N Robinson, Claus-Eric Ott
Osteoblastic differentiation is a multistep process characterized by osteogenic induction of mesenchymal stem cells, which then differentiate into proliferative pre-osteoblasts that produce copious amounts of extracellular matrix, followed by stiffening of the extracellular matrix, and matrix mineralization by hydroxylapatite deposition. Although these processes have been well characterized biologically, a detailed transcriptional analysis of murine primary calvaria osteoblast differentiation based on RNA sequencing (RNA-seq) analyses has not previously been reported...
April 10, 2018: Bone
https://www.readbyqxmd.com/read/29653141/dual-mechanism-of-type-vii-collagen-transfer-by-bone-marrow-mesenchymal-stem-cell-extracellular-vesicles-to-recessive-dystrophic-epidermolysis-bullosa-fibroblasts
#17
Jeffrey McBride, Luis Rodriguez-Menocal, Ambar Candanedo, Wellington Guzman, Marta Garcia-Contreras, Evangelos Badiavas
Recessive dystrophic epidermolysis bullosa (RDEB) is a severe blistering disease resulting from a lack of type VII collagen production. Recent clinical trials have shown efficacy of bone marrow-derived mesenchymal stem cells (BM-MSCs) in the treatment of epidermolysis bullosa, including improved basement membrane restructuring and cutaneous wound healing. The mechanism as to how type VII collagen is transferred from donor stem cell to recipient RDEB cells has not been defined. Here, we submit the model that BM-MSC-derived extracellular vesicles serve at least two roles: 1) to help transport type VII collagen within the extracellular space; and 2) to feed RDEB fibroblasts with messenger RNA that codes for type VII collagen, resulting in COL7A1 translation and synthesis of type VII collagen alpha chain proteins by RDEB fibroblasts...
April 10, 2018: Biochimie
https://www.readbyqxmd.com/read/29651914/inflammatory-pathways-are-central-to-posterior-cerebrovascular-artery-remodelling-prior-to-the-onset-of-congenital-hypertension
#18
Dawid Walas, Karol Nowicki-Osuch, Dominic Alibhai, Eva von Linstow Roloff, Jane Coghill, Christy Waterfall, Julian Fr Paton
Cerebral artery hypoperfusion may provide the basis for linking ischemic stroke with hypertension. Brain hypoperfusion may induce hypertension that may serve as an auto-protective mechanism to prevent ischemic stroke. We hypothesised that hypertension is caused by remodelling of the cerebral arteries, which is triggered by inflammation. We used a congenital rat model of hypertension and examined age-related changes in gene expression of the cerebral arteries using RNA sequencing. Prior to hypertension, we found changes in signalling pathways associated with the immune system and fibrosis...
January 1, 2018: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/29651107/circular-rna-circ-4099-is-induced-by-tnf-%C3%AE-and-regulates-ecm-synthesis-by-blocking-mir-616-5p-inhibition-of-sox9-in-intervertebral-disc-degeneration
#19
Hua Wang, Peiheng He, Hehai Pan, Jun Long, Jianru Wang, Zemin Li, Hui Liu, Weiying Jiang, Zhaomin Zheng
Circular RNAs (circRNAs) play important roles in the initiation and development of different diseases. Here, we detected their role in intervertebral disc (IVD) degeneration. An Arraystar human circular RNA microarray assay was used to detect circRNAs in normal and degenerated human IVD nucleus pulposus (NP) tissues. The role of circ-4099 in IVDD and its mechanism were evaluated by qRT-PCR and gain-of-function/loss-of-function studies. Interaction networks for competing endogenous RNAs (ceRNAs), miRNAs, and miRNA target gene were detected by bioinformatics analysis, RNA immunoprecipitation and luciferase assay...
April 13, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29643013/circulating-micrornas-predict-survival-of-patients-with-tumors-of-glial-origin
#20
Drusco A, P Fadda, Nigita G, Fassan M, Bottoni A, Gardiman Mp, D Sacchi, Calore F, Carosi M, Antenucci A, B Casini, Kelani H, Pescarmona E, Di Leva G, Zanesi N, Berger Ms, Croce Cm
The World Health Organization has recently introduced molecular prognostic-diagnostic biomarkers in the classification of Central Nervous System (CNS) tumors. In order to characterize subclasses of tumors that cannot find a precise location in the current classification, and, or cannot be tested because of scant material, it is important to find new molecular biomarkers in tissue and, or biological fluid samples. In this study, we identified serum microRNAs that could serve as biomarkers for the diagnosis and prognosis of patients with tumors of glial origin...
March 21, 2018: EBioMedicine
keyword
keyword
23473
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"