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Oocyte pick up

N Navali, A Gassemzadeh, L Farzadi, S Abdollahi, M Nouri, K Hamdi, F Mallah, F Jalilvand
STUDY QUESTION: Does the intrauterine administration of hCG immediately after oocyte retrieval in antagonist cycles with ICSI and fresh embryo transfer (ET) influence the implantation rate or chemical and clinical pregnancy rates? SUMMARY ANSWER: The intrauterine administration of hCG after oocyte retrieval increases the implantation rate and chemical and clinical pregnancy rates. WHAT IS KNOWN ALREADY: Over half of IVF/ICSI cycles fail due to implantation failure...
September 28, 2016: Human Reproduction
Alice Luddi, Angela Capaldo, Riccardo Focarelli, Martina Gori, Giuseppe Morgante, Paola Piomboni, Vincenzo De Leo
BACKGROUND: The status characterized by the imbalance between pro-oxidants and antioxidants molecules, defined as oxidative stress, has been suggested to be involved in the pathogenesis of subfertility in females. This study aims to evaluate the impact of a complete micronutrients supplementation on oxidative stress levels in follicular microenvironment as well as on in vitro fertilization (IVF) outcome. METHODS: This preliminary study was conducted between January 2014 and July 2015 at the Siena University Hospital Infertility Clinic...
2016: Reproductive Biology and Endocrinology: RB&E
Louise H Oliveira, Carlos P Sanches, Adriano S Seddon, Marcio B Veras, Flávio A Lima, Pedro L J Monteiro, Milo C Wiltbank, Roberto Sartori
The objective was to evaluate in vitro embryo production (IVEP) in nonlactating Holstein cows after ovarian superstimulation. Cows were randomly assigned in a crossover design to 1 of 2 groups: control (n = 35), which was not synchronized and not treated with hormones before ovum pick-up (OPU), or hormone-treated (n = 35), in which wave emergence was synchronized and animals treated with porcine (p)-FSH in the presence of norgestomet before OPU. In the hormone-treated group, all follicles ≥7 mm in diameter were aspirated for synchronization of wave emergence and cows received a norgestomet ear implant...
August 24, 2016: Journal of Dairy Science
Jei-Won Moon, Chung-Hoon Kim, So-Yun Park, Sung-Hoon Kim, Hee-Dong Chae, Byung-Moon Kang
In this retrospective cohort study, the effect of endometrial polypectomy carried out on the day of oocyte retrieval and on the first day of ovarian stimulation in patients with a large (≥10 mm) endometrial polyp undergoing IVF and intractyoplasmic sperm injection (ICSI) was investigated and compared. A total of 74 eligible IVF-ICSI cycles in 74 women who underwent endometrial polypectomy on either the day of oocyte pick-up (late polypectomy group, 39 cycles) or the first day of ovarian stimulation (early polypectomy group, 35 cycles) between January 2007 and July 2012 were included in this study...
September 2016: Reproductive Biomedicine Online
K Dafopoulos, C I Messini, G Anifandis, P Georgoulias, D Sourlas, I E Messinis
The aim of the present study was to investigate changes of blood ghrelin, adiponectin and resistin levels in IVF/ICSI-ET cycles. Twenty women were stimulated with recombinant FSH in a GnRH agonist short protocol for IVF/ICSI. Blood samples were taken on cycle day 2 before the commencement of injections, on cycle day 6 and on the days of HCG injection, oocyte pick up (OPU), embryo transfer (ET) as well as 7 and 12 days post-ET. Serum E2 levels increased during the stimulation, peaking on the HCG day and declined thereafter (p<0...
July 15, 2016: Physiological Research
Nezaket Kadıoğlu, Başak Güler, Sibel Özler, Sevtap Kılıç, M Nedim Çiçek, A Sertaç Batıoğlu
INTRODUCTION: Our aim in this study is to evaluate the effects of in vitro fertilization (IVF), including controlled ovarian hyperstimulation (COH) and the number of oocyte pick-up (OPU) procedures on the development of anti-ovarian antibodies (AOA). METHODS: To evaluate the effects of IVF procedures, namely, COH and OPU, serum samples for measuring AOA concentration levels by enzyme-linked immunosorbent assay were collected on the third day of the menstrual cycle, at the end of the COH, and after OPU...
August 2016: Advances in Therapy
Lanlan Fang, Yiping Yu, Ruizhe Zhang, Jingyan He, Ying-Pu Sun
Growth differentiation factor-8 (GDF-8) is found in the human serum, follicular fluid and granulosa cells. Our previous studies have shown that the human cumulus expansion and steroidogenesis can be regulated by GDF-8. However, thus far, the expression profile of GDF-8 in serum and whether the level of serum GDF-8 influences pregnancy results for patients treated with in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) is totally unknown. In this study, we showed that GDF-8 had a dynamic trend during controlled ovarian hyperstimulation (COH) procedure...
2016: Scientific Reports
Miro Kasum, Kristijan Kurdija, Slavko Orešković, Ermin Čehić, Dinka Pavičić-Baldani, Lana Škrgatić
The aim of the review is to analyse the combination of a gonadotrophin releasing hormone (GnRH) agonist with a human chorionic gonadotrophin (hCG) trigger, for final oocyte maturation in in vitro fertilisation (IVF) cycles. The concept being a ''dual trigger'' combines a single dose of the GnRH agonist with a reduced or standard dosage of hCG at the time of triggering. The use of a GnRH agonist with a reduced dose of hCG in high responders demonstrated luteal phase support with improved pregnancy rates, similar to those after conventional hCG and a low risk of ovarian hyperstimulation syndrome (OHSS)...
June 8, 2016: Gynecological Endocrinology
Yasuhiro Ogata, Guang-Min Yu, Takemasa Hidaka, Tadami Matzushige, Teruo Maeda
The low efficiency of embryo production in Holstein cows during early lactation presents many challenges for animal production. To improve its efficiency, the outcomes of single GnRH injections 48 hours before each of three cycles of ovum pick up (OPU; weeks 2, 4, and 6) were compared with three cycles of unstimulated OPU (controls; weeks 1, 3, and 5) in 35 Holstein cows during 6 weeks of early lactation (40-80 days postpartum). More total follicle numbers (19.5 vs. 16.0; P < 0.05) but fewer dominant follicles (0...
October 1, 2016: Theriogenology
Xiaokun Hu, Yingyi Luo, Kejun Huang, Yubing Li, Yanwen Xu, Canquan Zhou, Qingyun Mai
The aim of this study was to investigate 2 quantification criteria to evaluate the developmental condition of follicles cohort and clarify their impacts upon the determining of human chorionic gonadotropin trigger timing and the reproductive outcome: the proportion of mature follicles in growing follicles cohort on the day of human chorionic gonadotropin trigger and the peak estradiol level per oocyte on the day of human chorionic gonadotropin administration.Of the patients who underwent in vitro fertilization/ intracytoplasmic sperm injection-embryo transfer from 2011 to 2013, 492 controlled ovarian hyperstimulation cycles using gonadotropin-releasing hormone antagonists reaching the ovum pick-up and fresh embryo-transfer stage were included...
May 2016: Medicine (Baltimore)
Chi-Chun Liao, Robert Kuo-Kuang Lee, Shyr-Yeu Lin, Ming-Huei Lin, Yuh-Ming Hwu
OBJECTIVE: We aimed to compare the outcomes of in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatments in women of advanced age (>40 years) using anti-Müllerian hormone (AMH)-tailored ovarian stimulation protocols versus conventional protocols based on antral follicle count (AFC). MATERIALS AND METHODS: We retrospectively reviewed 210 women who underwent IVF/ICSI cycles: 116 women underwent stimulation protocols that were tailored to their AMH levels, whereas 94 women received treatment using conventional stimulation protocols based on AFC as the ovarian reserve marker...
April 2016: Taiwanese Journal of Obstetrics & Gynecology
Koji Nakagawa, Yayoi Nishi, Masayo Kaneyama, Rie Sugiyama, Hiroshi Motoyama, Rikikazu Sugiyama
BACKGROUND: The purpose of this study was to evaluate the effect of needle type on pain and bleeding during oocyte pick-up (OPU). METHODS: From May through November 2013, patients undergoing OPU from a single follicle without any analgesic treatment were including this study. Eligible patients (n=75) were randomized 1:1 to undergo the procedure with either a reduced needle (17 gauge body, 20 gauge tip; RN group) or a standard needle (19 gauge; SN group). Overall pain was assessed by patients using a visual analogue scale (VAS), and vaginal bleeding after the procedure was recorded...
October 2015: Journal of Reproduction & Infertility
Gyoung Hoon Lee, Hyun Jin Song, Kyu Sup Lee, Young Min Choi
OBJECTIVE: The number of assisted reproductive technology (ART) clinics, ART cycles, clinical pregnancy rate (CPR), and number of newborns conceived using ART have steadily increased in South Korea. This aim of this study was to describe the status of ART in South Korea between January 1 and December 31, 2011. METHODS: A localized online survey was created and sent to all available ART centers via email in 2015. Fresh embryo transfer (FET) cases were categorized depending on whether standard in vitro fertilization, intracytoplasmic sperm injection (ICSI), or half-ICSI procedures were used...
March 2016: Clinical and Experimental Reproductive Medicine
P Bárcena, M Rodríguez, A Obradors, V Vernaeve, R Vassena
STUDY QUESTION: Is there an optimal time to perform ICSI with respect to the times of oocyte pick-up (OPU), in order to maximize the reproductive outcomes in cycles with fresh and vitrified/warmed donor oocytes? SUMMARY ANSWER: We found no significant differences in reproductive outcomes of ICSI cycles within a wide range of times between OPU and ICSI. WHAT IS KNOWN ALREADY: In assisted reproduction, the oocyte is subject to denudation, vitrification/warming and ICSI...
June 2016: Human Reproduction
Ya-Hui Lin, Ke-Hsin Chueh, Jia-Ling Lin
AIMS AND OBJECTIVES: This study investigated the relationship between somatic symptoms, sleep disturbance and psychological distress in women who underwent oocyte pick-up and in vitro fertilisation-embryo transfer. BACKGROUND: According to worldwide research, women receiving assisted reproductive technologies may suffer from somatic and psychological symptoms and even experience sleep disturbance. Apparently, the guilt of infecundity forces Asian women to conceal this scenario and delay the time at which they accept medical assistance and mental support...
June 2016: Journal of Clinical Nursing
Ghylène Goudet, Cécile Douet, Aurore Kaabouba-Escurier, Isabelle Couty, Carla Moros-Nicolás, Philippe Barrière, Thierry Blard, Fabrice Reigner, Stefan Deleuze, Michèle Magistrini
Most wild and domestic donkey breeds are currently endangered or threatened. Their preservation includes the creation of a Genome Resource Bank. Embryos cryopreservation allows the preservation of genetics from both male and female and is the fastest method to restore a breed. Because embryo production in vivo is limited in equids, our objective was to establish conditions for in vitro production of embryos in donkey using ovum pick up (OPU), IVM, IVF, and in vitro culture of zygotes. Donkey cumulus-oocyte complexes (COCs) were collected by transvaginal ultrasound-guided aspirations OPU in adult cyclic jennies and in vitro matured in tissue culture medium 199 supplemented with fetal calf serum and epidermal growth factor for 24, 30, 34, or 38 hours...
July 15, 2016: Theriogenology
N Ghanem, J I Jin, S S Kim, B H Choi, K L Lee, A N Ha, S H Song, I K Kong
The current study investigated the possibility of using the AMH concentration as a predictor of the ability of Korean Hanwoo cows to produce cumulus-oocyte complexes, embryos that survive after transfer as well as the pregnancy outcome of surrogates. Eight sessions of ovum pick-up (OPU) were performed with 19 donor cows at an interval of 3-4 days. Antral follicle count (AFC), oocyte quality and in vitro embryo development were recorded for each cow. Embryos produced from cows with different AMH profiles were transferred into recipients (n = 96)...
April 2016: Reproduction in Domestic Animals, Zuchthygiene
E O S Batista, L M Vieira, M F Sá Filho, E A R Dias, B M Bayeux, M F Accorsi, F M Monteiro, A H Souza, P S Baruselli, M J D'Occhio
The aim of the present study was to evaluate small follicle number, oocyte yield, and in vitro embryo production (IVEP) in Zebu beef cows treated long term with a GnRH agonist to suppress ovarian follicular growth. Nelore (Bos indicus) cows (n = 20) showing regular estrous cycles were randomly assigned to one of two groups: control (n = 10, placebo ear implant without a GnRH agonist); GnRH agonist (n = 10, GnRH agonist ear implant containing 9.4-mg deslorelin). All cows underwent an ovum pick-up (OPU) session 14 days (Day 14) before the start of treatments (Day 0) followed by seven OPU-IVEP procedures at 30-day intervals (Days 0, 30, 60, 90, 120, 150, and 180)...
June 2016: Theriogenology
Francisca Martínez, Cayetana Barbed, Mónica Parriego, Miquel Solé, Ignacio Rodríguez, Buenaventura Coroleu
This is an observational study of the response to ovarian stimulation and preimplantational genetic screening (PGS) cycles of 188 patients with a foreseen high aneuploid rate, undergoing two or three stimulation cycles (2SC and 3SC) and oocyte vitrification to accumulate oocytes (Accumulation group = 112 patients) compared to patients undergoing one stimulation cycle (1SC Group= 76 patients) and fresh embryo transfer, between January 2011 and July 2014. Accumulation was performed when <10 MII oocytes were retrieved...
July 2016: Gynecological Endocrinology
Hiroshi Takenaka, Kazuki Nakao, Michiharu Horikawa, Hiroaki Negishi
We report a case of unnoticed pregnancy that was maintained during low estrogen and progesterone circumstances, that showed menses-like bleeding, and was then discovered after ovarian hyperstimulation during the next period. The patient was 39 years old and primigravid. She underwent intrauterine insemination, followed by luteal support with human chorionic gonadotrophin and progestin; however, she experienced menstruation-like bleeding 15 days later. As low estradiol and progesterone levels were confirmed on the 2nd day of bleeding, ovarian hyperstimulation of short protocol for in vitro fertilization was commenced...
April 2016: Journal of Obstetrics and Gynaecology Research
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