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https://www.readbyqxmd.com/read/29338139/occurrence-of-ovarian-follicular-dominance-during-stimulation-for-ivm-impacts-usable-blastocyst-yield
#1
Sergio Romero, Ricardo Pella, Francisco Escudero, Ygor Pérez, Mario García, Patricia Orihuela
OBJECTIVE: To evaluate the influence of ovarian follicular dominance on the outcome of oocyte in-vitro maturation. METHODS: This retrospective cohort study included 21 patients with polycystic ovaries or polycystic ovary syndrome (Rotterdam criteria, 2004) subjected to 24 in-vitro maturation (IVM) cycles between October 2015 and January 2017. Patients undergoing IVM received minimal gonadotropin stimulation starting on day 2 or 3 of the cycle; ovum pick-up typically occurred on days 6 to 8...
January 17, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29259492/intra-follicular-kisspeptin-levels-are-related-to-oocyte-maturation-and-gonadal-hormones-in-patients-who-are-undergoing-assisted-reproductive-technology
#2
Yuka Taniguchi, Akira Kuwahara, Ayaka Tachibana, Yuya Yano, Kiyohito Yano, Yuri Yamamoto, Mikio Yamasaki, Takeshi Iwasa, Kenji Hinokio, Toshiya Matsuzaki, Minoru Irahara
Purpose: To assess the kisspeptin concentrations in follicular fluid and their relationship with clinical outcomes during assisted reproductive technology. Methods: Thirty-nine patients who were aged 24-40 years and underwent oocyte retrieval for in vitro fertilization/intracytoplasmic sperm injection participated in this study. In 65 follicular fluid samples that had been obtained from 30 patients and their blood samples, the kisspeptin levels were measured in order to investigate the correlations with their gonadal hormone levels...
October 2017: Reproductive Medicine and Biology
https://www.readbyqxmd.com/read/29241919/does-the-freeze-all-policy-allow-for-a-better-outcome-in-assisted-reproductive-techniques-than-the-use-of-fresh-embryo-transfers-a%C3%A2-retrospective-study-on-cumulative-live-birth-rates
#3
Jui-Chun Chang, Ming-Jer Chen, Hwa-Fen Guu, Ya-Fang Chen, Yu-Chiao Yi, Hsiao-Fan Kung, Li-Yu Chen, Min-Min Chou
OBJECTIVE: There are increasing concerns regarding the adverse effects associated with control ovarian hyperstimulation (COH) in both endometrial and uterine environments. With the "segmentation treatment policy" of assisted reproductive techniques (ART), endometrial problems may be obviated through embryo cryopreservation. However, it remains unclear if the "freeze-all policy" offers a better outcome when compared with fresh embryo transfer (ET). To clarify this, we compared the cumulative live birth rates (CLBRs) between these two patient populations...
December 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29211889/reduced-live-birth-rates-after-ivf-icsi-in-women-with-previous-unilateral-oophorectomy-results-of-a-multicentre-cohort-study
#4
Tekla Lind, Jan Holte, Jan I Olofsson, Nermin Hadziosmanovic, Johannes Gudmundsson, Elizabeth Nedstrand, Mikael Lood, Lars Berglund, Kenny Rodriguez-Wallberg
STUDY QUESTION: Is there a reduced live-birth rate (LBR) after IVF/ICSI treatment in women with a previous unilateral oophorectomy (UO)? SUMMARY ANSWER: A significantly reduced LBR after IVF/ICSI was found in women with previous UO when compared with women with intact ovaries in this large multicentre cohort, both crudely and after adjustment for age, BMI, fertility centre and calendar period and regardless of whether the analysis was based on transfer of embryos in the fresh cycle only or on cumulative results including transfers using frozen-thawed embryos...
December 4, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29177245/controlled-ovarian-stimulation-with-r-fsh-plus-r-lh-vs-hmg-plus-r-fsh-in-patients-candidate-for-ivf-icsi-cycles-an-rct
#5
Ensieh Shahrokh Tehraninejad, Mina Farshbaf Taghinejad, Batool Hossein Rashidi, Fedyeh Haghollahi
Background: Different combination of gonadotropin preparation has been introduced with no definite superiority of one over others in in vitro fertilization (IVF), but individualized regimens for each patient are needed. Objective: The aim of the present study was to investigate the effect of controlled ovarian stimulation with recombinant- follicle stimulating hormone (r-FSH) plus recombinant-luteinizing hormone (rLH) versus human menopausal gonadotropin (HMG) plus r-FSH on fertility outcomes in IVF patients...
July 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29151336/efficacy-of-bispectral-index-monitoring-for-prevention-of-anestheticawareness-and-complications-during-oocyte-pick-up-procedure
#6
Aykut Urfalıoğlu, Mahmut Arslan, Murat Bakacak, Hafize Öksüz, Ömer Faruk Boran, Gözen Öksüz
Background/aim: This study was planned by considering that the use of bispectral index (BIS) monitoring ensures sufficient depth of anesthesia and avoids anesthetic awareness and patient movement in the oocyte pick-up (OPU) procedure.Materials and methods: Ninety-eight patients undergoing OPU were randomly divided into 2 groups as the control group (n = 48) and BIS group (n = 50). After propofol and remifentanil induction, the control group was given additional propofol according to reaction response, while the BIS group was given propofol at BIS values of 60 and above with the aim that BIS values be 40?60...
November 13, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/29132036/holding-immature-bovine-oocytes-in-a-commercial-embryo-holding-medium-high-developmental-competence-for-up-to-10%C3%A2-h-at-room-temperature
#7
Osvaldo Bogado Pascottini, Maaike Catteeuw, Ann Van Soom, Geert Opsomer
Bovine in vitro embryo production (IVP) following Ovum Pick Up (OPU) is all too often hampered by a large time gap between the harvest of oocytes of the first and last OPU session of the day. Immediately after retrieval, oocyte maturation is initiated, resulting in oocytes maturing at different time points which necessitates laborious scheduling of the IVP process. In this study, the potential of a commercial embryo holding medium (EHM; Syngro, Bioniche Inc.) to hold immature bovine oocytes was validated. We assessed the effect of holding time and temperature on (1) oocytes' maturation; (2) blastocyst development and quality at day 8 post insemination; and (3) blastocyst yield in small groups of oocytes/zygotes simulating OPU settings...
November 4, 2017: Theriogenology
https://www.readbyqxmd.com/read/29126090/the-vanishing-follicle-in-women-with-low-number-of-developing-follicles-during-assisted-reproduction
#8
Johnny S Younis, Shiran Yakovi, Ido Izhaki, Sami Haddad, Moshe Ben-Ami
OBJECTIVE: To investigate the occurrence of the "vanishing follicle" phenomenon in women with low number of developing follicles in assisted reproduction. STUDY DESIGN: Women with ≤ 6 follicles on the day of hCG administration with ≥ 14mm diameter were prospectively studied. Primary outcome measures were disappearance of ≥14mm and all-diameter follicles on the day of oocyte pick-up compared to the day of hCG administration. RESULTS: Among the 120 women recruited, 95 were found eligible and completed the study...
January 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29102132/effects-of-supplemental-calcium-salts-of-palm-oil-and-chromium-propionate-on-insulin-sensitivity-and-productive-and-reproductive-traits-of-mid-to-late-lactating-holstein-%C3%A3-gir-dairy-cows-consuming-excessive-energy
#9
T Leiva, R F Cooke, A P Brandão, R D Bertin, E A Colombo, V F B Miranda, L A C Lourenço, S M B Rodrigues, J L M Vasconcelos
This experiment compared insulin sensitivity, milk production, and reproductive outcomes in dairy cows consuming excessive energy during mid to late lactation and receiving in a 2 × 2 factorial design (1) concentrate based on ground corn (CRN; n = 20) or including 8% (DM basis) of Ca salts of palm oil (CSPO; n = 20), and (2) supplemented (n = 20) or not (n = 20) with 2.5 g/d of Cr-propionate. During the experiment (d 0-203), 40 multiparous, nonpregnant, lactating 3/4 Holstein × 1/4 Gir cows (initial days in milk = 81 ± 2; mean ± SE) were offered corn silage for ad libitum consumption, and individually received concentrate formulated to allow diets to provide 160% of their daily net energy for lactation requirements...
October 25, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29078197/source-treatment-and-type-of-nuclear-donor-cells-influences-in%C3%A2-vitro-and-in%C3%A2-vivo-development-of-embryos-cloned-by-somatic-cell-nuclear-transfer-in-camel-camelus-dromedarius
#10
Nisar A Wani, Seung B Hong
Experiments were conducted to evaluate the effect of source, treatment and type of nuclear donor cells on embryonic and fetal development of somatic cell nuclear-transfer reconstructs in dromedary camel. In experiment 1, actively growing, serum starved or confluent skin fibroblast cells were used as nuclear donors. In experiment 2, skin fibroblasts from 4 different animals while in experiment 3, skin fibroblasts and cumulus cells from the same animal were used as nuclear donors. In all the three experiments, mature oocytes collected by transvaginal ovum pick up were used as recipient cytoplasts...
October 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/29078039/effectiveness-of-seminal-plasma-in-ivf-treatment-a-systematic-review-and-meta-analysis
#11
Gabriele Saccone, Attilio Di Spiezio Sardo, Andrea Ciardulli, Claudia Caissutti, Marialuigia Spinelli, Daniel Surbek, Michael von Wolff
BACKGROUND: In in vitro fertilization (IVF) techniques only 20 to 25% of the transferred embryos lead to a pregnancy OBJECTIVE: To evaluate the beneficial effects of SP or semen applied at the time of oocyte aspiration or embryo transfer SEARCH STRATEGY: Electronic databases were searched from their inception until August 2017. SELECTION CRITERIA: We included all randomized controlled trials (RCTs) evaluating the effects of SP or semen in IVF treatment. Trials were considered if women were exposed to any kind of SP or semen (either SP/semen injection or sexual intercourse) around the time of oocyte pickup and embryo transfer...
October 27, 2017: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29040498/genome-stability-of-bovine-in-vivo-conceived-cleavage-stage-embryos-is-higher-compared-to-in-vitro-produced-embryos
#12
Olga Tšuiko, Maaike Catteeuw, Masoud Zamani Esteki, Aspasia Destouni, Osvaldo Bogado Pascottini, Urban Besenfelder, Vitezslav Havlicek, Katrien Smits, Ants Kurg, Andres Salumets, Thomas D'Hooghe, Thierry Voet, Ann Van Soom, Joris Robert Vermeesch
STUDY QUESTION: Is the rate and nature of chromosome instability (CIN) similar between bovine in vivo-derived and in vitro-cultured cleavage-stage embryos? SUMMARY ANSWER: There is a major difference regarding chromosome stability of in vivo-derived and in vitro-cultured embryos, as CIN is significantly lower in in vivo-derived cleavage-stage embryos compared to in vitro-cultured embryos. WHAT IS KNOWN ALREADY: CIN is common during in vitro embryogenesis and is associated with early embryonic loss in humans, but the stability of in vivo-conceived cleavage-stage embryos remains largely unknown...
November 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28988321/apoptotic-biomarkers-in-cumulus-cells-in-relation-to-embryo-quality-in-polycystic-ovary-syndrome
#13
Ensieh Salehi, Reza Aflatoonian, Ashraf Moeini, Nazila Yamini, Ebrahim Asadi, Zahra Khosravizadeh, Masoumeh Dehghan Tarzjani, Zhila Naghibi Harat, Farid Abolhassani
PURPOSE: To investigate associations between gene expression pattern of apoptotic biomarkers in cumulus cells of polycystic ovary syndrome (PCOS) patients and the quality of oocytes and embryos. METHODS: 40 intracytoplasmic sperm injection patients, of whom 20 were PCOS and 20 were healthy women, were included in this study. Serum hormone levels were measured using Radioimmunoassay for each patient. The expression of survivin, caspase-3, and caspase-7 in 200 cumulus complexes surrounding mature oocytes (100 in PCOS versus 100 in control groups) collected individually at pick up was examined by real-time polymerase chain reaction (real-time PCR)...
December 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28960429/the-effects-of-low-dose-human-chorionic-gonadotropin-combined-with-human-menopausal-gonadotropin-protocol-on-women-with-hypogonadotropic-hypogonadism-undergoing-ovarian-stimulation-for-in-vitro-fertilization
#14
Shutian Jiang, Yanping Kuang
OBJECTIVES: To investigate the effects of low-dose human chorionic gonadotropin (hCG) combined with human menopausal gonadotropin (HMG) protocol on cycle characteristics and outcomes of infertile women with hypogonadotropic hypogonadism (HH) undergoing ovarian stimulation for in vitro fertilization (IVF). DESIGN: A retrospective cohort study. SETTING: Tertiary-care academic medical centre. PATIENT(S): Forty-six infertile patients with HH and seventy-one infertile patients with tubal factor (TF) infertility undergoing IVF...
January 2018: Clinical Endocrinology
https://www.readbyqxmd.com/read/28929977/exposure-to-follicular-fluid-during-oocyte-maturation-and-oviductal-fluid-during-post-maturation-does-not-improve-in-vitro-embryo-production-in-the-horse
#15
Cécile Douet, Olivia Parodi, Nicola Antonio Martino, Giovanni Michele Lacalandra, Michele Nicassio, Fabrice Reigner, Stefan Deleuze, Maria Elena Dell'Aquila, Ghylène Goudet
Most wild equids and many domestic horse breeds are at risk of extinction, so there is an urgent need for genome resource banking. Embryos cryopreservation allows the preservation of genetics from male and female and is the fastest method to restore a breed. In the equine, embryo production in vitro would allow the production of several embryos per cycle. Intracytoplasmic sperm injection (ICSI) is used to generate horse embryos, but it requires expensive equipment and expertise in micromanipulation, and blastocyst development rates remain low...
September 20, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28837025/comparison-of-two-different-dosage-of-gnrh-agonist-as-ovulation-trigger-in-oocyte-donors-a-randomized-controled-trial
#16
Sonia Morales Zarcos, Pamela Valdivieso Mejía, Carla Donado Stefani, Pascual Sánchez Martin, Fernando Sánchez Martin
OBJECTIVE: To compare the results obtained with two different GnRH agonist dosages: 0.3mg versus 0.4mg to trigger ovulation in oocyte donor cycles. METHODS: Experimental controlled randomized trial including 40 patients from a private practice center. The patients were randomized into two groups. Group A received a single dose of Triptorelin 0.3mg (Decapeptyl®) 36hours before pick-up. Group B patients received Triptorelin 0.4mg (Decapeptyl®) before pick-up to final oocyte maturation...
September 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28822904/the-effect-of-age-and-length-of-gonadotropin-stimulation-on-the-in%C3%A2-vitro-embryo-development-of-holstein-calf-oocytes
#17
Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Werner Glanzner, Yasmin Schuermann, Rodrigo C Bohrer, Naomi Dicks, Paulo R da Rosa, Matheus P De Cesaro, Rosalba Lopez, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Jim Gourdon, Hernan Baldassarre, Vilceu Bordignon
The use of oocytes recovered from prepubertal donors for in vitro embryo production has great potential for accelerating the rate of genetic gain in the dairy industry. However, these oocytes are known to be less developmentally competent than those from adult donors. In this study, we investigated the effect of age and gonadotropin stimulation in Holstein heifers subjected to oocyte collection every two weeks between 2 and 6 months of age. In order to assess the effect of gonadotropin stimulation, animals were subjected to one of three treatments, namely Short (ST; 36-42 h), Long (LT; ≥72 h) and No Treatment (NT) prior to laparoscopic ovum pick-up (LOPU)...
August 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/28822459/oral-propylene-glycol-modifies-follicular-fluid-and-gene-expression-profiles-in-cumulus-oocyte-complexes-and-embryos-in-feed-restricted-heifers
#18
G Gamarra, C Ponsart, S Lacaze, F Nuttinck, A Cordova, P Mermillod, B Marquant-Le Guienne, D Monniaux, P Humblot, A A Ponter
Dietary supplementation with propylene glycol (PG) increases in vitro production of high-quality embryos in feed-restricted heifers. The aim of the present study was to evaluate the effects of PG in feed-restricted heifers on follicular fluid insulin and insulin-like growth factor (IGF) 1 concentrations, expression of IGF system genes in oocytes and cumulus cells and the expression of selected genes in blastocysts. Feed-restricted (R) heifers were drenched with water or PG during induced oestrous cycles (400mL of PG or water/drench, daily drenching at 1600 hours for the first 9 days of the oestrous cycle)...
August 21, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28663739/impact-of-mid-luteal-phase-gnrh-agonist-administration-on-reproductive-outcomes-in-gnrh-agonist-triggered-cycles-a-randomized-controlled-trial
#19
Abdelhamid Benmachiche, Sebti Benbouhedja, Abdelali Zoghmar, Amel Boularak, Peter Humaidan
OBJECTIVE: To explore whether the addition of a mid-luteal bolus of GnRH agonist (GnRHa) improves the implantation rate (IR) in in vitro fertilization (IVF) cycles. DESIGN: A randomized controlled trial. SETTING: Private IVF center. PATIENTS: 328 IVF/intracytoplasmic sperm injection patients were triggered with GnRHa and received 1,500 IU HCG on the day of oocyte pick-up (OPU) in addition to a standard luteal phase support (LPS)...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28662882/ovum-pick-up-interval-in-buffalo-bubalus-bubalis-managed-under-wetland-conditions-in-argentina-effect-on-follicular-population-oocyte-recovery-and-in-vitro-embryo-development
#20
J Konrad, G Clérico, M J Garrido, G Taminelli, M Yuponi, R Yuponi, G Crudeli, M Sansinena
The excellent adaptation of water buffalo (Bubalis bubalis) to swampy environments means that animals are frequently managed in areas with restricted access for reproductive procedures. The objective of this study was to evaluate the effect of the ovum pick-up (OPU) interval on follicular population, oocyte recovery, oocyte quality and in vitro embryo production. Twelve Murrah buffaloes were subjected to two consecutive dominant follicle reductions, and randomly assigned to either 7-day (n=6) or 14-day (n=6) OPU interval groups...
June 20, 2017: Animal Reproduction Science
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