keyword
MENU ▼
Read by QxMD icon Read
search

Cartilage biology

keyword
https://www.readbyqxmd.com/read/29331090/enhanced-chondrogenesis-of-mesenchymal-stem-cells-over-silk-fibroin-chitosan-chondroitin-sulfate-three-dimensional-scaffold-in-dynamic-culture-condition
#1
Parinita Agrawal, Krishna Pramanik, Varshini Vishwanath, Amit Biswas, Akalabya Bissoyi, Pradeep Kumar Patra
Chondroitin sulfate (Ch) is one of the main structural components of cartilage tissue, therefore, its presence in tissue engineered scaffold is expected to enhance cartilage regeneration. Previously, silk fibroin/chitosan (SF/CS) blend was proven to be a potential biomaterial for tissue development. In this study, the effect of Ch on physicochemical and biological properties of SF/CS blend was investigated and scaffolds with 0.8 wt% Ch was found to be favorable. The scaffolds possess pore size of 37-212 µm, contact angle 46...
January 13, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29323144/defining-tissue-proteomes-by-systematic-literature-review
#2
Sarah A Hibbert, Matiss Ozols, Christopher E M Griffiths, Rachel E B Watson, Mike Bell, Michael J Sherratt
Defining protein composition is a key step in understanding the function of both healthy and diseased biological systems. There is currently little consensus between existing published proteomes in tissues such as the aorta, cartilage and organs such as skin. Lack of agreement as to both the number and identity of proteins may be due to issues in protein extraction, sensitivity/specificity of detection and the use of disparate tissue/cell sources. Here, we developed a method combining bioinformatics and systematic review to screen >32M articles from the Web of Science for evidence of proteins in healthy human skin...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29320528/developmental-finite-element-analysis-of-cichlid-pharyngeal-jaws-quantifying-the-generation-of-a-key-innovation
#3
Tim Peterson, Gerd B Müller
Advances in imaging and modeling facilitate the calculation of biomechanical forces in biological specimens. These factors play a significant role during ontogenetic development of cichlid pharyngeal jaws, a key innovation responsible for one of the most prolific species diversifications in recent times. MicroCT imaging of radiopaque-stained vertebrate embryos were used to accurately capture the spatial relationships of the pharyngeal jaw apparatus in two cichlid species (Haplochromis elegans and Amatitlania nigrofasciata) for the purpose of creating a time series of developmental stages using finite element models, which can be used to assess the effects of biomechanical forces present in a system at multiple points of its ontogeny...
2018: PloS One
https://www.readbyqxmd.com/read/29316363/exploiting-advanced-hydrogel-technologies-to-address-key-challenges-in-regenerative-medicine
#4
REVIEW
Daniel A Foyt, Michael D A Norman, Tracy T L Yu, Eileen Gentleman
Regenerative medicine aims to tackle a panoply of challenges from repairing focal damage to articular cartilage to preventing pathological tissue remodeling after myocardial infarction. Hydrogels are water-swollen networks formed from synthetic or naturally derived polymers and are emerging as important tools to address these challenges. Recent advances in hydrogel chemistries are enabling researchers to create hydrogels that can act as 3D ex vivo tissue models, allowing them to explore fundamental questions in cell biology by replicating tissues' dynamic and nonlinear physical properties...
January 9, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29313037/soluble-collagen-vi-treatment-enhances-mesenchymal-stem-cells-expansion-for-engineering-cartilage
#5
Piera Smeriglio, Jieun Lee, Nidhi Bhutani
Bone Marrow-derived mesenchymal stem cells (BM-MSC) are an attractive source for cell-based therapies in cartilage injury owing to their efficient differentiation into chondrocytes and their immune-suppressive abilities. However, their clinical use is hampered by a scarcity of cells leading to compromised efficacy. While expansion of human MSC ex vivo can potentially overcome the scarcity of cells, current methods lead to a rapid loss of the stem cell properties. In this study, we report soluble Collagen VI (cartilage pericellular matrix component) as a potential biologic that can expand the MSC population while maintaining the stem cell phenotype as confirmed by expression of the stem cell markers CD105 and CD90...
September 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/29305998/effects-of-mechanical-loading-on-cortical-defect-repair-using-a-novel-mechanobiological-model-of-bone-healing
#6
Chao Liu, Robert Carrera, Vittoria Flamini, Lena Kenny, Pamela Cabahug-Zuckerman, Benson M George, Daniel Hunter, Bo Liu, Gurpreet Singh, Philipp Leucht, Kenneth A Mann, Jill A Helms, Alesha B Castillo
Mechanical loading is an important aspect of post-surgical fracture care. The timing of load application relative to the injury event may differentially regulate repair depending on the stage of healing. Here, we use a novel mechanobiological model of cortical defect repair that offers several advantages including its technical simplicity and spatially confined repair program, making effects of both physical and biological interventions more easily assessed. Using this model, we show that daily loading (5N peak load, 2Hz, 60 cycles, 4 consecutive days) during hematoma consolidation and inflammation disrupts the injury site and activates cartilage formation on the periosteal surface adjacent to the defect...
January 3, 2018: Bone
https://www.readbyqxmd.com/read/29305974/circular-rna-atp9b-a-competing-endogenous-rna-regulates-the-progression-of-osteoarthritis-by-targeting-mir-138-5p
#7
Zhi-Bin Zhou, Di Du, Gao-Xiang Huang, Aimin Chen, Lei Zhu
Osteoarthritis (OA) is the most common joint disease and is mainly characterized by degradation of the articular cartilage. Recently, circular RNAs (circRNAs), novel noncoding RNAs with different biological functions and pathological implications, have been reported to be closely associated with various diseases. Growing evidence indicates that circRNAs act as competing endogenous RNAs (ceRNAs) that bind with microRNAs (miRNAs) and regulate their downstream functions. Here, we identified a new circRNA, circRNA_Atp9b, and further investigated its function in OA using a well-established mouse chondrocyte model...
January 3, 2018: Gene
https://www.readbyqxmd.com/read/29302607/mandibular-explant-assay-for-investigating-extrinsic-stimuli-on-bone-and-cartilage-development
#8
Jamie Jiang, Jessica W Bertol, Walid D Fakhouri
A major issue in developmental biology is to determine how embryonic tissues respond to molecular signals in a timely manner and given the position-restricted instructions during morphogenesis, of which Meckel's cartilage is a classical example. The ex-vivo explant model is a practical and convenient system that allows investigation of bone and cartilage responses to specific stimuli under a controlled manner that closely mimics the in vivo conditions. In this protocol, the explant model was applied to test whether Meckel's cartilage and surrounding tissues are responsive to the Endothelin1 (Edn1) signaling molecule and whether it would rescue the phenotype of genetic mutations...
December 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/29300225/articular-cartilage-injury-pathways-and-treatment-options
#9
Timothy M Simon, Douglas W Jackson
Articular cartilage injury and degeneration is a frequent occurrence in synovial joints. Treatment of these articular cartilage lesions are a challenge because this tissue is incapable of quality repair and/or regeneration to its native state. Nonoperative treatments endeavor to control symptoms, and include anti-inflammatory medication, viscosupplementation, bracing, orthotics, and activity modification. Techniques to stimulate the intrinsic repair (fibrocartilage) process include drilling, abrasion, and microfracture of the subchondral bone...
March 2018: Sports Medicine and Arthroscopy Review
https://www.readbyqxmd.com/read/29295700/physical-cues-drive-chondrogenic-differentiation
#10
Qi Zhang, Shiyu Lin, Jinfeng Liao, Xiaoxiao Cai
BACKGROUND: Cellular differentiation occurs in a complicated microenvironment containing multiple components including soluble factors and physical cues. In addition to biochemical composition, physical cues are also crucial in determining cellular behaviors. OBJECTIVE: To better understand the interaction between physical signals and cells, we discuss the effects of physical cues on cellular behaviors, especially chondrogenic differentiation in vitro. Furthermore, the mechanisms by which these physical signals are transmitted from the extracellular matrix into the cell are also considered...
January 2, 2018: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29280278/bioadhesive-polymersome-for-localized-and-sustained-drug-delivery-at-pathological-sites-with-harsh-enzymatic-and-fluidic-environment-via-supramolecular-host-guest-complexation
#11
Meiling Zhu, Kongchang Wei, Sien Lin, Xiaoyu Chen, Chia-Ching Wu, Gang Li, Liming Bian
Targeted and sustained delivery of drugs to diseased tissues/organs, where body fluid exchange and catabolic activity are substantial, is challenging due to the fast cleansing and degradation of the drugs by these harsh environmental factors. Herein, a multifunctional and bioadhesive polycaprolactone-β-cyclodextrin (PCL-CD) polymersome is developed for localized and sustained co-delivery of hydrophilic and hydrophobic drug molecules. This PCL-CD polymersome affords multivalent crosslinking action via surface CD-mediated host-guest interactions to generate a supramolecular hydrogel that exhibits evident shear thinning and efficient self-healing behavior...
December 27, 2017: Small
https://www.readbyqxmd.com/read/29277342/do-immune-cells-lead-the-way-in-subchondral-bone-disturbance-in-osteoarthritis
#12
REVIEW
Adrian Weber, Pok Man Boris Chan, Chunyi Wen
Osteoarthritis (OA) is a whole-joint disorder, and non-cartilage articular pathologies, e.g. subchondral bone disturbance, contribute substantially to the onset and progression of the disease. In the early stage of OA, abnormal mechanical loading leads to micro-cracks or micro-fractures that trigger a reparative process with angiogenesis and inflammatory response. With the progression of disease, cystic lesion, sclerosis and osteophytosis occur at tissue level, and osteoblast dysfunction at cellular level. Osteoblasts derived from OA sclerotic bone produce increased amount of type I collagen with aberrant Col1A1/A2 ratio and poor mineralization capability...
December 22, 2017: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/29276788/a-role-for-soluble-il-6-receptor-in-osteoarthritis
#13
Graham Akeson, Charles J Malemud
Interleukin-6 (IL-6) is one of several pro-inflammatory cytokines present at elevated levels in the synovial fluid of individuals with confirmed clinical diagnosis of rheumatoid arthritis (RA) and osteoarthritis (OA). The mechanism of action of IL-6 was shown to involve its capacity to interact with a membrane-bound IL-6 receptor (mIL-6Rα), also known as the "classical" IL-6 pathway, or through its interaction with a soluble IL-6 receptor (sIL-6R) termed the "trans-signaling" pathway. Activation of downstream signaling is transduced via these IL-6 receptors and principally involves the Janus Kinase/Signal Transduction and Activators of Transcription (JAK/STAT) signaling pathway that is further regulated by glycoprotein-130 (gp130) interacting with the IL-6/mIL-6R complex...
2017: Journal of Functional Morphology and Kinesiology
https://www.readbyqxmd.com/read/29274624/interferon-regulatory-factor-5-irf5-regulates-the-expression-of-matrix-metalloproteinase-3-mmp-3-in-human-chondrocytes
#14
Lin Guo, Ruihu Hao, Fengde Tian, Ning An, Kunzheng Wang
Matrix metalloproteinase-3 (MMP-3) plays a pivotal role in the destruction of articular cartilage in osteoarthritis (OA). The regulation of gene expression of MMP-3 is complicated. Interferon regulatory factor 5 (IRF5) is a member of the interferon regulatory factor family of transcription factors. Little information regarding the biological function of IRF5 on chondrocytes and the pathogenesis of OA has been reported. In the current study, for the first time, we report that IRF5 is expressed in human primary chondrocytes and human chondrosarcoma cell line SW1353 cells...
December 20, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29273645/stratification-of-knee-osteoarthritis-two-major-patient-subgroups-identified-by-genome-wide-expression-analysis-of-articular-cartilage
#15
Jamie Soul, Sara L Dunn, Sanjay Anand, Ferdinand Serracino-Inglott, Jean-Marc Schwartz, Ray P Boot-Handford, Tim E Hardingham
INTRODUCTION: Osteoarthritis (OA) is a heterogeneous and complex disease. We have used a network biology approach based on genome-wide analysis of gene expression in OA knee cartilage to seek evidence for pathogenic mechanisms that may distinguish different patient subgroups. METHODS: Results from RNA-Sequencing (RNA-Seq) were collected from intact knee cartilage at total knee replacement from 44 patients with OA, from 16 additional patients with OA and 10 control patients with non-OA...
December 22, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/29273221/a-modular-approach-to-creating-large-engineered-cartilage-surfaces
#16
Audrey C Ford, Wan Fung Chui, Anne Y Zeng, Aditya Nandy, Ellen Liebenberg, Carlo Carraro, Galateia Kazakia, Tamara Alliston, Grace D O'Connell
Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis. Tissue engineering has provided a promising biological treatment strategy that is currently being evaluated in clinical trials. However, current approaches in translating these techniques to developing large engineered tissues remains a significant challenge. In this study, we present a method for developing large-scale engineered cartilage surfaces through modular fabrication. Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels...
December 9, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29261554/the-utility-of-biologics-osteotomy-and-cartilage-restoration-in-the-knee
#17
Rachel M Frank, Eric J Cotter, Eric J Strauss, Andreas H Gomoll, Brian J Cole
The management of complex cartilage and meniscal pathology in young, athletic patients is extremely challenging. Joint preservation surgery is most difficult in patients with concomitant knee pathologies, including cartilage defects, meniscal deficiency, malalignment, and/or ligamentous insufficiency. Clinical decision making for these patients is further complicated by articular cartilage lesions, which often are incidental findings; therefore, treatment decisions must be based on the confirmed contribution of articular cartilage lesions to symptomatology...
January 1, 2018: Journal of the American Academy of Orthopaedic Surgeons
https://www.readbyqxmd.com/read/29258882/transcriptome-comparison-of-meniscus-from-patients-with-and-without-osteoarthritis
#18
Robert H Brophy, Bo Zhang, Lei Cai, Rick W Wright, Linda J Sandell, Muhammad Farooq Rai
OBJECTIVE: To assess the impact of osteoarthritis (OA) on the meniscus by comparing transcripts and biological processes in the meniscus between patients with and without OA. DESIGN: RNA microarrays were used to identify transcripts differentially expressed (DE) in meniscus obtained from 12 OA and 12 non-OA patients. The non-OA specimens were obtained at the time of arthroscopic partial meniscectomy (APM). Real-time PCR was performed on selected transcripts. Biological processes and gene-networking was examined computationally...
December 16, 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29250494/diagnosis-and-treatment-of-inflammatory-joint-disease
#19
REVIEW
Yeesuk Kim, Hyun-Cheol Oh, Jang Won Park, In-Sung Kim, Jun-Young Kim, Ki-Choul Kim, Dong-Sik Chae, Woo-Lam Jo, Joo-Hyoun Song
Arthritis damages the cartilage within joints, resulting in degenerative changes, including loss of function and joint instability. Ankylosing spondylitis (AS) is a chronic inflammatory condition affecting the spine and bone-to-tendon attachment area within the sacroiliac joint leading to back pain and progressive spinal stiffness. In the final stages, AS causes hyperkyphosis-a condition closely tied to the human leukocyte antigen-B27 gene. Rheumatoid arthritis is a chronic, systemic autoimmune disease characterized by the simultaneous inflammation of the synovium of multiple joints, leading to joint damage (e...
December 2017: Hip & Pelvis
https://www.readbyqxmd.com/read/29247798/circular-rna-expression-profile-of-articular-chondrocytes-in-an-il-1%C3%AE-induced-mouse-model-of-osteoarthritis
#20
Zhibin Zhou, Di Du, Aimin Chen, Lei Zhu
Osteoarthritis (OA) is a widely prevalent degenerative joint disease characterized by articular cartilage degradation and joint inflammation. The pathogenesis of OA remains unclear, leading to a lack of effective treatment. Previous studies have reported that circular RNAs (circRNAs) are involved in the development of various diseases. However, the function of circRNAs and their roles in OA is largely unknown. Therefore, we aimed to investigate changes in circRNA expression and predict their functions in OA by using bioinformatics analysis...
December 13, 2017: Gene
keyword
keyword
23439
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"