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Cartilage biology

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https://www.readbyqxmd.com/read/29792508/effects-of-tgf-%C3%AE-overexpression-via-raav-gene-transfer-on-the-early-repair-processes-in-an-osteochondral-defect-model-in-minipigs
#1
Magali Cucchiarini, Ann-Kathrin Asen, Lars Goebel, Jagadeesh K Venkatesan, Gertrud Schmitt, David Zurakowski, Michael D Menger, Matthias W Laschke, Henning Madry
BACKGROUND: Application of the chondrogenic transforming growth factor beta (TGF-β) is an attractive approach to enhance the intrinsic biological activities in damaged articular cartilage, especially when using direct gene transfer strategies based on the clinically relevant recombinant adeno-associated viral (rAAV) vectors. PURPOSE: To evaluate the ability of an rAAV-TGF-β construct to modulate the early repair processes in sites of focal cartilage injury in minipigs in vivo relative to control (reporter lacZ gene) vector treatment...
May 1, 2018: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29792383/donor-variation-and-optimization-of-human-mesenchymal-stem-cells-chondrogenesis-in-hyaluronic-acid
#2
Minwook Kim, Isaac E Erickson, Alice H Huang, Sean T Garrity, Robert L Mauck, David R Steinberg
Mesenchymal stem cells (MSCs) are an attractive cell type for cartilage repair that can undergo chondrogenesis in a variety of three dimensional (3D) scaffolds. Hyaluronic acid (HA) hydrogels provide a biologically relevant interface for cell encapsulation. While our previous studies have shown that MSC-laden HA constructs can mature in vitro to match native mechanical properties using cells from animal sources, clinical application will depend on the successful translation of these findings to human cells...
May 24, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29785051/hyaluronan-negatively-regulates-vascular-calcification-involving-bmp2-signaling
#3
Yonglun Kong, Qingchun Liang, Yanting Chen, Pingzhen Yang, Xiaoyu Liu, Yining Li, Siyuan Feng, Ji Wu, Wantao Liu, Jingyi Tang, Huimin Yu, Jing-Song Ou, Lihe Lu, Jianyun Yan
Vascular calcification is a highly regulated biological process similar to bone formation involving osteogenic differentiation of vascular smooth muscle cells (VSMCs). Hyaluronan (HA), a major structural component of the extracellular matrix in cartilage, has been shown to inhibit osteoblast differentiation. However, whether HA affects osteogenic differentiation and calcification of VSMCs remains unclear. In the present study, we used in vitro and ex vivo models of vascular calcification to investigate the role of HA in vascular calcification...
May 21, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29775732/il4-10-fusion-protein-has-chondroprotective-anti-inflammatory-and-potentially-analgesic-effects-in-the-treatment-of-osteoarthritis
#4
C Steen-Louws, J Popov-Celeketic, S C Mastbergen, K Coeleveld, C E Hack, N Eijkelkamp, M Tryfonidou, S Spruijt, J A G van Roon, F P J G Lafeber
OBJECTIVE: Effective disease-modifying drugs for osteoarthritis (DMOAD) should preferably have chondroprotective, anti-inflammatory, and analgesic activity combined in a single molecule. We developed a fusion protein of IL4 and IL10 (IL4-10 FP), in which the biological activity of both cytokines is preserved. The present study evaluates the chondroprotective, anti-inflammatory, and analgesic activity of IL4-10 FP in in vitro and in vivo models of osteoarthritis. METHODS: Human osteoarthritic cartilage tissue and synovial tissue were cultured with IL4-10 FP...
May 15, 2018: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29772338/hyaluronic-acid-an-efficient-biomacromolecule-for-treatment-of-inflammatory-skin-and-joint-diseases-a-review-of-recent-developments-and-critical-appraisal-of-preclinical-and-clinical-investigations
#5
REVIEW
Li Hui Chen, Jian Feng Xue, Zhi Yong Zheng, Muhammad Shuhaidi, Hnin Ei Thu, Zahid Hussain
Hyaluronic acid (HA) plays multifaceted role in regulating various biological processes and maintaining homeostasis into the body. Numerous researches evidenced the biomedical implications of HA in skin repairmen, cancer prognosis, wound healing, tissue regeneration, anti-inflammatory, immunomodulation. The present review was aimed to summarize and critically appraise the recent developments and efficacy of HA for treatment of inflammatory skin and joint diseases. A thorough analysis of the literature revealed that HA based formulations (i...
May 14, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29771958/cooperation-of-bmp-and-ihh-signaling-in-interdigital-cell-fate-determination
#6
Arunima Murgai, Sara Altmeyer, Stephanie Wiegand, Przemko Tylzanowski, Sigmar Stricker
The elaborate anatomy of hands and feet is shaped by coordinated formation of digits and regression of the interdigital mesenchyme (IM). A failure of this process causes persistence of interdigital webbing and consequently cutaneous syndactyly. Bone morphogenetic proteins (BMPs) are key inductive factors for interdigital cell death (ICD) in vivo. NOGGIN (NOG) is a major BMP antagonist that can interfere with BMP-induced ICD when applied exogenously, but its in vivo role in this process is unknown. We investigated the physiological role of NOG in ICD and found that Noggin null mice display cutaneous syndactyly and impaired interdigital mesenchyme specification...
2018: PloS One
https://www.readbyqxmd.com/read/29756586/the-review-of-nanomaterials-inducing-the-differentiation-of-stem-cells-into-chondrocyte-phenotypes-in-cartilage-tissue-engineering
#7
Xueping Xie, Qi Zhang, Tengfei Zhou, Quanquan Ma, Liao JinFeng
Cartilage, as a nanostructured tissue, because of its awfully poor capacity for inherent regeneration and complete hierarchical structure, is severely difficult to regenerate after damages. Tissue engineering methods have provided great contribution for cartilage repair. Nanomaterials have special superiority in regulating stem cell behaviors due to their special mechanical and biological properties and biomimetic characteristics, therefore, they have been given great attention in tissue regeneration. Nanomaterials are divided into organic and inorganic nanomaterials...
May 11, 2018: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29756585/animal-models-used-for-testing-hydrogels-in-cartilage-regeneration
#8
Chuntie Zhu, Qiong Wu, Xu Zhang, Fubo Chen, Xiyang Liu, Qixiang Yang, Lei Zhu
Focal cartilage or osteochondral lesions can be painful and detrimental. Besides pain and limited function of joints, cartilage defect is considered as one of the leading extrinsic risk factors for osteoarthritis (OA). Thus, clinicians and scientists have paid great attention to regenerative therapeutic methods for the early treatment of cartilaginous defects. Regenerative medicine, showing great hope for regenerating cartilage tissue, rely on the combination of biodegradable scaffolds and specific biological cues, such as growth factors, adhesive factors and genetic materials...
May 14, 2018: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29753378/microrna-140-suppresses-human-chondrocytes-hypertrophy-by-targeting-smad1-and-controlling-the-bone-morphogenetic-protein-pathway-in-osteoarthritis
#9
Canfeng Li, Qinshen Hu, Zhuo Chen, Bin Shen, Jing Yang, Pengde Kang, Zongke Zhou, Fuxing Pei
BACKGROUND: This study aimed to investigate the expression levels and relationship of bone morphogenetic proteins (BMPs) signaling molecules and microRNA-140 (miR-140) in human osteoarthritis (OA) chondrocytes. MATERIALS AND METHODS: Different stage chondrocytes (normal cartilage, mid-stage OA and advanced-stage OA) were isolated from cartilage samples according to Kellgren and Lawrence criteria. The effect of miR-140 on BMPs signaling was evaluated by transfecting miR-140 mimic or inhibitor into chondrocytes...
May 2018: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/29749546/serum-proteomic-analysis-of-the-anti%C3%A2-arthritic-effects-of-sinomenine-on-rats-with-collagen%C3%A2-induced-arthritis
#10
Xin Qian, Zhiming Zhao, Wei Shang, Zhihan Xu, Beibei Zhang, Hui Cai
Rheumatoid arthritis (RA) is an autoimmune disease, which is characterized by inflammatory synovitis, and the subsequent destruction of articular cartilage and bone. Sinomenine is a traditional Chinese medicine, which has been employed as a clinical treatment for RA for several years in China. The present study investigated the anti‑arthritic effects of sinomenine on Sprague‑Dawley rats with collagen‑induced arthritis (CIA). The differentially expressed proteins in serum were measured by proteomic analysis in order to generate a differentially expressed protein network...
May 3, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29741735/widespread-epigenomic-transcriptomic-and-proteomic-differences-between-hip-osteophytic-and-articular-chondrocytes-in-osteoarthritis
#11
Julia Steinberg, Roger A Brooks, Lorraine Southam, Sahir Bhatnagar, Theodoros I Roumeliotis, Konstantinos Hatzikotoulas, Eleni Zengini, J Mark Wilkinson, Jyoti S Choudhary, Andrew W McCaskie, Eleftheria Zeggini
Objectives: To identify molecular differences between chondrocytes from osteophytic and articular cartilage tissue from OA patients. Methods: We investigated genes and pathways by combining genome-wide DNA methylation, RNA sequencing and quantitative proteomics in isolated primary chondrocytes from the cartilaginous layer of osteophytes and matched areas of low- and high-grade articular cartilage across nine patients with OA undergoing hip replacement surgery. Results: Chondrocytes from osteophytic cartilage showed widespread differences to low-grade articular cartilage chondrocytes...
May 8, 2018: Rheumatology
https://www.readbyqxmd.com/read/29739464/in-vivo-cartilage-tissue-engineering
#12
B Gurer, S Cabuk, O Karakus, N Yilmaz, C Yilmaz
BACKGROUND: Biologic treatment options for cartilage injuries require chondrocyte expansion using cell culture. Clinical application is accomplished in two surgical sessions and is expensive. If isolation of chondrocytes and stimulus for proliferation and extracellular matrix synthesis can be achieved in vivo, the treatment can be performed in one session and the cost can be reduced. METHODS: A 2.5-cm diameter full-thickness chondral defect was created in the knees of five groups of sheep...
May 8, 2018: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29736578/enhancing-biological-and-biomechanical-fixation-of-osteochondral-scaffold-a-grand-challenge
#13
Maryam Tamaddon, Chaozong Liu
Osteoarthritis (OA) is a degenerative joint disease, typified by degradation of cartilage and changes in the subchondral bone, resulting in pain, stiffness and reduced mobility. Current surgical treatments often fail to regenerate hyaline cartilage and result in the formation of fibrocartilage. Tissue engineering approaches have emerged for the repair of cartilage defects and damages to the subchondral bones in the early stage of OA and have shown potential in restoring the joint's function. In this approach, the use of three-dimensional scaffolds (with or without cells) provides support for tissue growth...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29736571/clinical-management-in-early-oa
#14
Rita Grazina, Renato Andrade, Ricardo Bastos, Daniela Costa, Rogério Pereira, José Marinhas, António Maestro, João Espregueira-Mendes
Knee osteoarthritis affects an important percentage of the population throughout their life. Several factors seem to be related to the development of knee osteoarthritis including genetic predisposition, gender, age, meniscal deficiency, lower limb malalignments, joint instability, cartilage defects, and increasing sports participation. The latter has contributed to a higher prevalence of early onset of knee osteoarthritis at younger ages with this active population demanding more consistent and durable outcomes...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29736568/emerging-concepts-in-treating-cartilage-osteochondral-defects-and-osteoarthritis-of-the-knee-and-ankle
#15
Hélder Pereira, Ibrahim Fatih Cengiz, Carlos Vilela, Pedro L Ripoll, João Espregueira-Mendes, J Miguel Oliveira, Rui L Reis, C Niek van Dijk
The management and treatment of cartilage lesions, osteochondral defects, and osteoarthritis remain a challenge in orthopedics. Moreover, these entities have different behaviors in different joints, such as the knee and the ankle, which have inherent differences in function, biology, and biomechanics. There has been a huge development on the conservative treatment (new technologies including orthobiologics) as well as on the surgical approach. Some surgical development upraises from technical improvements including advanced arthroscopic techniques but also from increased knowledge arriving from basic science research and tissue engineering and regenerative medicine approaches...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29736302/rheumatoid-arthritis-pathological-mechanisms-and-modern-pharmacologic-therapies
#16
REVIEW
Qiang Guo, Yuxiang Wang, Dan Xu, Johannes Nossent, Nathan J Pavlos, Jiake Xu
Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease that primarily affects the lining of the synovial joints and is associated with progressive disability, premature death, and socioeconomic burdens. A better understanding of how the pathological mechanisms drive the deterioration of RA progress in individuals is urgently required in order to develop therapies that will effectively treat patients at each stage of the disease progress. Here we dissect the etiology and pathology at specific stages: (i) triggering, (ii) maturation, (iii) targeting, and (iv) fulminant stage, concomitant with hyperplastic synovium, cartilage damage, bone erosion, and systemic consequences...
2018: Bone Research
https://www.readbyqxmd.com/read/29736142/medical-ozone-therapy-as-a-potential-treatment-modality-for-regeneration-of-damaged-articular-cartilage-in-osteoarthritis
#17
REVIEW
Sello Lebohang Manoto, Makwese Johaness Maepa, Shirley Keolebogile Motaung
Osteoarthritis (OA) is the most common degenerative joint disease and a growing health problem affecting more than half of the population over the age of 65. It is characterized by inflammation in the cartilage and synovium, resulting in the loss of joint structure and progressive damage to the cartilage. Many pro-inflammatory mediators are elevated in OA, including reactive oxygen species (ROS) such as nitric oxide (NO) and hydrogen peroxide (H2 O2 ). Damaged articular cartilage remains a challenge to treat due to the limited self-healing capacity of the tissue and unsuccessful biological interventions...
May 2018: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/29733109/synthesis-characterization-molecular-modeling-and-biological-evaluation-of-thieno-pyrimidinone-methanesulphonamide-thio-derivatives-as-non-steroidal-anti-inflammatory-agents
#18
Adriana Carol Eleonora Graziano, Giovanna Pannuzzo, Ettore Salemi, Andrea Santagati, Rosanna Avola, Emanuele Longo, Venera Cardile
The present study reports the synthesis, molecular docking and biological evaluation of eight (5-8 and 5a-8a) newly synthesized thieno-pyrimidinone methanesulphonamide thio-derivatives. The synthetic route used to prepare the new isomers thioaryl and thio-cycloesyl derivatives of the heterocyclic system 6-phenylthieno[3,2]pyrimidinone was economically and environmentally very advantageous and characterized by the simplicity of procedure, reduction of isolation steps, purification phases, time, costs, and waste production...
May 7, 2018: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/29729853/microscale-frictional-strains-determine-chondrocyte-fate-in-loaded-cartilage
#19
Edward D Bonnevie, Michelle L Delco, Lena R Bartell, Naveen Jasty, Itai Cohen, Lisa A Fortier, Lawrence J Bonassar
Mounting evidence suggests that altered lubricant levels within synovial fluid have acute biological consequences on chondrocyte homeostasis. While these responses have been connected to increased friction, the mechanisms behind this response remain unknown. Here, we combine a frictional bioreactor with confocal elastography and image-based cellular assays to establish the link between cartilage friction, microscale shear strain, and acute, adverse cellular responses. Our incorporation of cell-scale strain measurements reveals that elevated friction generates high shear strains localized near the tissue surface, and that these elevated strains are closely associated with mitochondrial dysfunction, apoptosis, and cell death...
April 25, 2018: Journal of Biomechanics
https://www.readbyqxmd.com/read/29729762/editorial-commentary-the-all-epiphyseal-anterior-cruciate-ligament-distal-femoral-approach-sockets-or-tunnels
#20
EDITORIAL
Frank A Cordasco
I believe that the distal femoral approach for anterior cruciate ligament reconstruction in the skeletally immature athlete with 3 to 6 years of remaining growth is best performed with an all-inside, all-epiphyseal technique using sockets rather than an outside-in approach creating tunnels. A shorter socket rather than a longer tunnel exposes a smaller surface area of the lateral distal femoral physis to potential compromise and resultant valgus malalignment. In addition, exiting the lateral femoral aspect of the epiphysis with a full-diameter tunnel as compared with a smaller diameter drill hole used to prepare a socket places the posterior articular cartilage, the lateral collateral ligament and anterolateral ligament footprints, and the popliteus tendon insertion at risk...
May 2018: Arthroscopy: the Journal of Arthroscopic & related Surgery
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