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https://www.readbyqxmd.com/read/28449499/regional-dietary-characteristics-and-bronchial-foreign-body-a-repeated-misdiagnosis-caused-by-a-red-pepper
#1
Yutian Lai, Jian Huang, Xudong Zhou, Heng Du, Guowei Che
Bronchial foreign body is a common health problem worldwide, types of bronchial foreign bodies are variegated, depending on patient age and cultural background including religious beliefs as well as alimentary habits. However, misdiagnoses caused by regional dietary correlation factors were rarely concerned. In this case, a 52-year-old woman with aggravated cough and hemosputum took thoracic CT scan twice and flexible fiberoptic bronchoscopy 5 times, which all revealed the chronic pulmonary inflammation. Furthermore, the biopsies showed bronchial granulation nodule formation...
March 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28449405/the-impact-of-lesion-location-on-dysphagia-incidence-pattern-and-complications-in-acute-stroke-part-2-oropharyngeal-residue-swallow-and-cough-response-and-pneumonia
#2
S Suntrup-Krueger, A Kemmling, T Warnecke, C Hamacher, S Oelenberg, T Niederstadt, W Heindel, H Wiendl, R Dziewas
BACKGROUND AND PURPOSE: Dysphagia is a well-known complication of acute stroke. Given the complexity of cerebral swallowing control it is still difficult to predict which patients are likely to develop swallowing dysfunction based on their neuroimaging. In Part 2 of a comprehensive voxel-based imaging study, whether the location of a stroke lesion can be correlated with further dysfunctional swallowing patterns, pulmonary protective reflexes and pneumonia was evaluated. METHODS: In all, 200 acute stroke cases were investigated applying flexible endoscopic evaluation of swallowing within 96 h from admission...
April 27, 2017: European Journal of Neurology: the Official Journal of the European Federation of Neurological Societies
https://www.readbyqxmd.com/read/28449391/flexible-semitransparent-and-inorganic-resistive-memory-based-on-bati0-95-co0-05-o3-film
#3
Yuxi Yang, Guoliang Yuan, Zhibo Yan, Yaojin Wang, Xubing Lu, Jun-Ming Liu
Perovskite ceramics and single crystals are commonly hard and brittle due to their small maximum elastic strain. Here, large-scale BaTi0.95 Co0.05 O3 (BTCO) film with a SrRuO3 (SRO) buffered layer on a 10 µm thick mica substrate is flexible with a small bending radius of 1.4 mm and semitransparent for visible light at wavelengths of 500-800 nm. Mica/SRO/BTCO/Au cells show bipolar resistive switching and the high/low resistance ratio is up to 50. The resistive-switching properties show no obvious changes after the 2...
April 27, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28449263/multi-dice-r-package-for-comparative-population-genomic-inference-under-hierarchical-co-demographic-models-of-independent-single-population-size-changes
#4
Alexander T Xue, Michael J Hickerson
Population genetic data from multiple taxa can address comparative phylogeographic questions about community-scale response to environmental shifts, and a useful strategy to this end is to employ hierarchical co-demographic models that directly test multi-taxa hypotheses within a single, unified analysis while benefiting in statistical power from aggregating datasets. This approach has been applied to classical phylogeographic datasets such as mitochondrial barcodes as well as reduced-genome polymorphism datasets that can yield 10,000s of SNPs, produced by emergent technologies such as RAD-seq and GBS...
April 27, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28449258/improve-toolmarks-impressions-in-soft-wax
#5
Nir Finkelstein, Nikolai Volkov, Tsadok Tsach
When the forensic toolmarks laboratory receives for examination and comparison a tool that is suspected of having been involved in a crime, the expert performs tests designed to determine whether or not the specific tool generates the same toolmarks as those found at the crime scene. This is performed by testing tool striation on a piece of soft metal, such as lead, and examining the marks left by the tool. Studies have shown that wax may be an optimal material for this purpose. This study examines the use of wax at different temperatures and shows that quality of results is better when the wax is cooled (recommended temperature is -18°C)...
May 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28449031/rnablueprint-flexible-multiple-target-nucleic-acid-sequence-design
#6
Stefan Hammer, Birgit Tschiatschek, Christoph Flamm, Ivo L Hofacker, Sven Findeiß
Motivation: Realizing the value of synthetic biology in biotechnology and medicine requires the design of molecules with specialized functions. Due to its close structure to function relationship, and the availability of good structure prediction methods and energy models, RNA is perfectly suited to be synthetically engineered with predefined properties. However, currently available RNA design tools cannot be easily adapted to accommodate new design specifications. Furthermore, complicated sampling and optimization methods are often developed to suit a specific RNA design goal, adding to their inflexibility...
April 25, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28448957/static-vs-dynamic-dft-prediction-of-ir-spectra-of-flexible-molecules-in-the-condensed-phase-the-clcf2cf-cf3-ocf2ch3-liquid-as-a-test-case
#7
REVIEW
Daria Ruth Galimberti, Alberto Milani, Marie-Pierre Gaigeot, Stefano Radice, Claudio Tonelli, Rosaldo Picozzi, Chiara Castiglioni
First-principles molecular dynamics (FPMD) simulations in the framework of Density Functional Theory (DFT) are carried out for the prediction of the infrared spectrum of the fluorinated molecule ClCF2CF(CF3)OCF2CH3 in liquid and gas phase. This molecule is characterized by a flexible structure, allowing the co-existence of several stable conformers, that differ by values of the torsional angles. FPMD computed spectra are compared to the experimental ones, and to Boltzmann weighted IR spectra based on gas phase calculations...
April 17, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28448922/affine-and-non-affine-deformations-quantified-in-cytoskeletal-networks-through-three-dimensional-form-finding-model
#8
Yifan Wang, Jinghai Gong, Denis Wirtz, Benjamin W Schafer
Actin filaments and cross-linkers are main components of cytoskeletal networks in eukaryotic cells, and they support bending moments and axial forces respectively. A three-dimensional form-finding model is proposed in this work to investigate affine and non-affine deformations in cytoskeletal networks. In recent studies, modeling of cytoskeletal networks turns out to be a key piece in the cell mechanics puzzle. We used form-finding analysis to compute and analyze cytoskeletal models. A three-dimensional model is much more flexible and contains more elements than a two-dimensional model, and non-linear finite element analysis is difficult to converge...
April 20, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28448827/dopamine-cognitive-biases-and-assessment-of-certainty-a-neurocognitive-model-of-delusions
#9
REVIEW
Annabel Broyd, Ryan P Balzan, Todd S Woodward, Paul Allen
This paper examines the evidence that delusions can be explained within the framework of a neurocognitive model of how the brain assesses certainty. Here, 'certainty' refers to both low-level interpretations of one's environment and high-level (conscious) appraisals of one's beliefs and experiences. A model is proposed explaining how the brain systems responsible for assigning certainty might dysfunction, contributing to the cause and maintenance of delusional beliefs. It is suggested that delusions arise through a combination of perturbed striatal dopamine and aberrant salience as well as cognitive biases such as the tendency to jump to conclusions (JTC) and hypersalience of evidence-hypothesis matches...
April 18, 2017: Clinical Psychology Review
https://www.readbyqxmd.com/read/28448796/reconciling-epigenetic-memory-and-transcriptional-responsiveness
#10
Amanda G Fisher, Michael P H Stumpf, Matthias Merkenschlager
The molecular basis of cellular memory is important but poorly understood. Using estimates of histone dynamics, Martin Howard and colleagues construct a mathematical model that helps to explain both the stability and flexibility of Polycomb-mediated gene regulation in cellular memory.
April 26, 2017: Cell Systems
https://www.readbyqxmd.com/read/28448543/heterologous-expression-of-a-thermophilic-diacylglycerol-acyltransferase-triggers-triglyceride-accumulation-in-escherichia-coli
#11
Beatriz Lázaro, Juan A Villa, Omar Santín, Matilde Cabezas, Cintia D F Milagre, Fernando de la Cruz, Gabriel Moncalián
Triglycerides (TAGs), the major storage molecules of metabolic energy and source of fatty acids, are produced as single cell oil by some oleogenic microorganisms. However, these microorganisms require strict culture conditions, show low carbon source flexibilities, lack efficient genetic modification tools and in some cases pose safety concerns. TAGs have essential applications such as behaving as a source for added-value fatty acids or giving rise to the production of biodiesel. Hence, new alternative methods are urgently required for obtaining these oils...
2017: PloS One
https://www.readbyqxmd.com/read/28448453/working-memory-in-the-prefrontal-cortex
#12
REVIEW
Shintaro Funahashi
The prefrontal cortex participates in a variety of higher cognitive functions. The concept of working memory is now widely used to understand prefrontal functions. Neurophysiological studies have revealed that stimulus-selective delay-period activity is a neural correlate of the mechanism for temporarily maintaining information in working memory processes. The central executive, which is the master component of Baddeley's working memory model and is thought to be a function of the prefrontal cortex, controls the performance of other components by allocating a limited capacity of memory resource to each component based on its demand...
April 27, 2017: Brain Sciences
https://www.readbyqxmd.com/read/28448017/low-density-primary-hippocampal-neuron-culture
#13
Reiko T Roppongi, Kevin P Champagne-Jorgensen, Tabrez J Siddiqui
The ability to probe the structure and physiology of individual nerve cells in culture is crucial to the study of neurobiology, and allows for flexibility in genetic and chemical manipulation of individual cells or defined networks. Such ease of manipulation is simpler in the reduced culture system when compared to the intact brain tissue. While many methods for the isolation and growth of these primary neurons exist, each has its own limitations. This protocol describes a method for culturing low-density and high-purity rodent embryonic hippocampal neurons on glass coverslips, which are then suspended over a monolayer of glial cells...
April 18, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28448014/an-efficient-and-flexible-cell-aggregation-method-for-3d-spheroid-production
#14
Sarah M Maritan, Eric Y Lian, Lois M Mulligan
Monolayer cell culture does not adequately model the in vivo behavior of tissues, which involves complex cell-cell and cell-matrix interactions. Three-dimensional (3D) cell culture techniques are a recent innovation developed to address the shortcomings of adherent cell culture. While several techniques for generating tissue analogues in vitro have been developed, these methods are frequently complex, expensive to establish, require specialized equipment, and are generally limited by compatibility with only certain cell types...
March 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447998/characterization-of-ultra-fine-grained-and-nanocrystalline-materials-using-transmission-kikuchi-diffraction
#15
Gwénaëlle Proust, Patrick Trimby, Sandra Piazolo, Delphine Retraint
One of the challenges in microstructure analysis nowadays resides in the reliable and accurate characterization of ultra-fine grained (UFG) and nanocrystalline materials. The traditional techniques associated with scanning electron microscopy (SEM), such as electron backscatter diffraction (EBSD), do not possess the required spatial resolution due to the large interaction volume between the electrons from the beam and the atoms of the material. Transmission electron microscopy (TEM) has the required spatial resolution...
April 1, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447983/high-temperature-fabrication-of-nanostructured-yttria-stabilized-zirconia-ysz-scaffolds-by-in-situ-carbon-templating-xerogels
#16
Sixbert P Muhoza, Matthew A Cottam, Michael D Gross
We demonstrate a method for the high temperature fabrication of porous, nanostructured yttria-stabilized-zirconia (YSZ, 8 mol% yttria - 92 mol% zirconia) scaffolds with tunable specific surface areas up to 80 m(2)·g(-1). An aqueous solution of a zirconium salt, yttrium salt, and glucose is mixed with propylene oxide (PO) to form a gel. The gel is dried under ambient conditions to form a xerogel. The xerogel is pressed into pellets and then sintered in an argon atmosphere. During sintering, a YSZ ceramic phase forms and the organic components decompose, leaving behind amorphous carbon...
April 16, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447981/isolation-characterization-and-purification-of-macrophages-from-tissues-affected-by-obesity-related-inflammation
#17
Joselyn N Allen, Adwitia Dey, Ruth Nissly, James Fraser, Shan Yu, Gayathri Balandaram, Jeffrey M Peters, Pamela A Hankey-Giblin
Obesity promotes a chronic inflammatory state that is largely mediated by tissue-resident macrophages as well as monocyte-derived macrophages. Diet-induced obesity (DIO) is a valuable model in studying the role of macrophage heterogeneity; however, adequate macrophage isolations are difficult to acquire from inflamed tissues. In this protocol, we outline the isolation steps and necessary troubleshooting guidelines derived from our studies for obtaining a suitable population of tissue-resident macrophages from mice following 18 weeks of high-fat (HFD) or high-fat/high-cholesterol (HFHCD) diet intervention...
April 3, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447972/wild-type-blocking-pcr-combined-with-direct-sequencing-as-a-highly-sensitive-method-for-detection-of-low-frequency-somatic-mutations
#18
Adam Z Albitar, Wanlong Ma, Maher Albitar
Accurate detection and identification of low frequency mutations can be problematic when assessing residual disease after therapy, screening for emerging resistance mutations during therapy, or when patients have few circulating tumor cells. Wild-type blocking PCR followed by sequencing analysis offers high sensitivity, flexibility, and simplicity as a methodology for detecting these low frequency mutations. By adding a custom designed locked nucleic acid oligonucleotide to a new or previously established conventional PCR based sequencing assay, sensitivities of approximately 1 mutant allele in a background of 1,000 WT alleles can be achieved (1:1,000)...
March 29, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447839/the-whorfian-time-warp-representing-duration-through-the-language-hourglass
#19
Emanuel Bylund, Panos Athanasopoulos
How do humans construct their mental representations of the passage of time? The universalist account claims that abstract concepts like time are universal across humans. In contrast, the linguistic relativity hypothesis holds that speakers of different languages represent duration differently. The precise impact of language on duration representation is, however, unknown. Here, we show that language can have a powerful role in transforming humans' psychophysical experience of time. Contrary to the universalist account, we found language-specific interference in a duration reproduction task, where stimulus duration conflicted with its physical growth...
April 27, 2017: Journal of Experimental Psychology. General
https://www.readbyqxmd.com/read/28447822/social-cognition-internalized-stigma-and-recovery-orientation-among-adults-with-serious-mental-illness
#20
Katherine C Cunningham, Alicia Lucksted
OBJECTIVE: The social-cognitive model is useful in understanding internalized stigma, but research has not examined it in relationship to recovery orientation, an important outcome. This study examined the impact of the four stages of internalized stigma on recovery orientation and assessed cognitive insight as a moderator. METHOD: Data from a community sample of adults with serious mental illness (N = 268) were collected through structured interviews. Regression-based analyses were used to examine the main effects of internalized stigma on recovery orientation and the moderating effect of cognitive insight...
April 27, 2017: Psychiatric Rehabilitation Journal
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