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https://www.readbyqxmd.com/read/29782238/generation-of-a-lineage-ii-powassan-virus-deer-tick-virus-cdna-clone-assessment-of-flaviviral-genetic-determinants-of-tick-and-mosquito-vector-competence
#1
Joan L Kenney, Michael Anishchenko, Meghan Hermance, Hannah Romo, Ching-I Chen, Saravanan Thangamani, Aaron C Brault
The Flavivirus genus comprises a diverse group of viruses that utilize a wide range of vertebrate hosts and arthropod vectors. The genus includes viruses that are transmitted solely by mosquitoes or vertebrate hosts as well as viruses that alternate transmission between mosquitoes or ticks and vertebrates. Nevertheless, the viral genetic determinants that dictate these unique flaviviral host and vector specificities have been poorly characterized. In this report, a cDNA clone of a flavivirus that is transmitted between ticks and vertebrates (Powassan lineage II, deer tick virus [DTV]) was generated and chimeric viruses between the mosquito/vertebrate flavivirus, West Nile virus (WNV), were constructed...
May 21, 2018: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/29780749/-rhipicephalus-bursa-sialotranscriptomic-response-to-blood-feeding-and-babesia-ovis-infection-identification-of-candidate-protective-antigens
#2
Sandra Antunes, Joana Couto, Joana Ferrolho, Fábio Rodrigues, João Nobre, Ana S Santos, M Margarida Santos-Silva, José de la Fuente, Ana Domingos
Ticks are among the most prevalent blood-feeding arthropods, and they act as vectors and reservoirs for numerous pathogens. Sialotranscriptomic characterizations of tick responses to blood feeding and pathogen infections can offer new insights into the molecular interplay occurring at the tick-host-pathogen interface. In the present study, we aimed to identify and characterize Rhipicephalus bursa salivary gland (SG) genes that were differentially expressed in response to blood feeding and Babesia ovis infection...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29778634/genetic-characterization-of-anaplasma-marginale-strains-from-tunisia-using-single-and-multiple-gene-typing-reveals-novel-variants-with-an-extensive-genetic-diversity
#3
Mourad Ben Said, Alaa Ben Asker, Hanène Belkahia, Raoua Ghribi, Rachid Selmi, Lilia Messadi
Anaplasma marginale, which is responsible for bovine anaplasmosis in tropical and subtropical regions, is a tick-borne obligatory intraerythrocytic bacterium of cattle and wild ruminants. In Tunisia, information about the genetic diversity and the phylogeny of A. marginale strains are limited to the msp4 gene analysis. The purpose of this study is to investigate A. marginale isolates infecting 16 cattle located in different bioclimatic areas of northern Tunisia with single gene analysis and multilocus sequence typing methods on the basis of seven partial genes (dnaA, ftsZ, groEL, lipA, secY, recA and sucB)...
May 12, 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29777337/molecular-epidemiology-of-parasitic-protozoa-and-ehrlichia-canis-in-wildlife-in-madrid-central-spain
#4
Angel Criado-Fornelio, T Martín-Pérez, C Verdú-Expósito, S A Reinoso-Ortiz, J Pérez-Serrano
Wildlife species are involved in the transmission of diverse pathogens. This study aimed to monitor raccoons (Procyon lotor), American minks (Neovison vison), and red foxes (Vulpes vulpes) as potential reservoirs in central Spain. Specifically, 200 spleen and fecal samples (from 194 raccoons, 3 minks, and 3 foxes) were analyzed molecularly by PCR/qPCR and sequencing for the presence of piroplasmids, Hepatozoon spp., Toxoplasma gondii, and Ehrlichia canis infections in the Community of Madrid (Spain). Biological samples were obtained in the years 2014, 2015, and 2016...
May 18, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29776392/molecular-identification-of-tick-borne-pathogens-in-asymptomatic-individuals-with-human-immunodeficiency-virus-type-1-hiv-1-infection-a-retrospective-study
#5
Renata Welc-Falęciak, Justyna D Kowalska, Małgorzata Bednarska, Magdalena Szatan, Agnieszka Pawełczyk
BACKGROUND: The studies on the occurrence and diversity of tick-borne infections in HIV-infected individuals have been few, and the subject has been relatively neglected when compared with other common infections associated with HIV. In HIV-positive patients in whom a serological diagnostics is complicated due to reduced positive predictive value, a method where the microorganism is detected directly is of great value. Therefore, we performed a molecular study to ascertain the prevalence and incidence of tick-borne infections in HIV-infected persons in Poland, an endemic area for Ixodes ricinus ticks...
May 18, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29776377/shifts-in-borrelia-burgdorferi-s-l-geno-species-infections-in-ixodes-ricinus-over-a-10-year-surveillance-period-in-the-city-of-hanover-germany-and-borrelia-miyamotoi-specific-reverse-line-blot-detection
#6
Katrin Blazejak, Marie-Kristin Raulf, Elisabeth Janecek, Daniela Jordan, Volker Fingerle, Christina Strube
BACKGROUND: Lyme borreliosis caused by spirochetes of the Borrelia burgdorferi (sensu lato) complex is still the most common tick-borne disease in Europe, posing a considerable threat to public health. The predominant vector in Europe is the widespread hard tick Ixodes ricinus, which also transmits the relapsing fever spirochete B. miyamotoi as well as pathogenic Rickettsiales (Anaplasma phagocytophilum, Rickettsia spp.). To assess the public health risk, a long-term monitoring of tick infection rates with the named pathogens is indispensable...
May 18, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29774285/molecular-prevalence-of-babesia-bigemina-in-rhipicephalus-microplus-ticks-infesting-cross-bred-cattle-of-punjab-india
#7
S A Bhat, N K Singh, H Singh, S S Rath
Babesiosis is an economically important tick-borne apicomplexan protozoan disease of cattle in tropical and subtropical regions. In the present study, Rhipicephalus microplus engorged female ticks were collected from 135 apparently healthy cattle from different agro-climatic zones of Punjab, India, to investigate the carrier status of Babesia bigemina infection in vector tick by using microscopy and PCR based assays. PCR when applied on DNA extracted from the egg masses harvested from ticks showed 1.48% (2/135) samples as positive, whereas 4...
August 2017: Parasite Epidemiology and Control
https://www.readbyqxmd.com/read/29772759/human-babesiosis-caused-by-babesia-duncani-has-widespread-distribution-across-canada
#8
John D Scott, Catherine M Scott
Human babesiosis caused by Babesia duncani is an emerging infectious disease in Canada. This malaria-like illness is brought about by a protozoan parasite infecting red blood cells. Currently, controversy surrounds which tick species are vectors of B. duncani. Since the availability of a serological or molecular test in Canada for B. duncani has been limited, we conducted a seven-year surveillance study (2011⁻2017) to ascertain the occurrence and geographic distribution of B. duncani infection country-wide...
May 17, 2018: Healthcare (Basel, Switzerland)
https://www.readbyqxmd.com/read/29771344/prevalence-of-rickettsia-species-rickettsiales-rickettsiaceae-in-dermacentor-variabilis-ticks-acari-ixodidae-in-north-carolina
#9
Madhavi L Kakumanu, Loganathan Ponnusamy, Haley Sutton, Steven R Meshnick, William L Nicholson, Charles S Apperson
The American dog tick, Dermacentor variabilis (Say), is a vector of spotted fever group (SFG) rickettsiae, including Rickettsia rickettsii the causative organism of Rocky Mountain spotted fever (RMSF). In North Carolina, SFG rickettsioses (including RMSF) are a leading cause of tick-borne illness. Knowledge of the infection rate and geographic distribution of D. variabilis ticks infected with Rickettsia spp. provides information on the spatial distribution of public health risk. Accordingly, we extracted genomic DNA from adult D...
May 16, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29770944/analysis-of-pastoralists-perception-on-challenges-and-opportunities-for-sheep-and-goat-production-in-northern-kenya
#10
Mohamed Haji Abdilatif, Joshua Orungo Onono, Florence Kanini Mutua
Small ruminants' production contributes to livelihood of pastoral communities, but this faces myriad constraints. This study aimed at identifying challenges facing producers of small ruminants, prioritizing diseases and their control measures and documenting opportunities for improvement. Sixteen focus group discussions with livestock owners and 13 key informant interviews were done in selected areas in Mandera County, Northern Kenya, and both quantitative and qualitative data collected using a questionnaire guide...
May 16, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29769359/study-scale-determines-whether-wildlife-loss-protects-against-or-promotes-tick-borne-disease
#11
J C Buck, S E Perkins
No abstract text is available yet for this article.
May 16, 2018: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29769356/comment-on-titcomb-et-al-s-interacting-effects-of-wildlife-loss-and-climate-on-ticks-and-tick-borne-disease
#12
H J Esser, N A Hartemink, W F de Boer
No abstract text is available yet for this article.
May 16, 2018: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29766270/parasitological-and-molecular-diagnostic-of-a-clinical-babesia-caballi-outbreak-in-southern-romania
#13
Mariana Ionita, Isabela Madalina Nicorescu, Kurt Pfister, Ioan Liviu Mitrea
Equine piroplasmosis (EP) is a tick-borne disease of equids caused by Babesia caballi and/or Theileria equi, which is endemic in many tropical and temperate areas of the world. However, clinical outbreaks of EP in Romania during the last decades have not been reported Therefore, the aim of this paper is (i) to describe a clinical B. caballi outbreak in horses on several farms in Southern Romania using a diagnostic and therapeutic approach and (ii) the molecular diagnostic of EP in an endemic area of Romania...
May 15, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29763485/what-s-eating-you-ixodes-tick-and-related-diseases-part-2-diagnosis-and-treatment-of-regional-tick-borne-diseases
#14
Kelsey D Wilson, Dirk M Elston
The Ixodes tick is an important arthropod vector in the transmission of human disease. Although Lyme disease is the most prevalent zoonosis transmitted by Ixodes ticks, other less common diseases may be encountered, including human granulocytic anaplasmosis, babesiosis, Powassan virus infection, tick-borne encephalitis, Borrelia miyamotoi disease, and tick paralysis. In part 2 of this review, disease presentation, diagnosis, and treatment of these less commonly encountered tick-borne diseases are discussed.
April 2018: Cutis; Cutaneous Medicine for the Practitioner
https://www.readbyqxmd.com/read/29762747/detection-of-babesia-spp-in-dogs-and-their-ticks-from-peninsular-malaysia-emphasis-on-babesia-gibsoni-and-babesia-vogeli-infections-in-rhipicephalus-sanguineus-sensu-lato-acari-ixodidae
#15
Batah Kunalan Prakash, Van Lun Low, Wei Yin Vinnie-Siow, Tiong Kai Tan, Yvonne Ai-Lian Lim, Akhavan Rezaei Morvarid, Sazaly AbuBakar, Mohd Sofian-Azirun
Canine babesiosis is an emerging tick-borne disease with a worldwide distribution, including Malaysia. While the prevalence of Babesia has been documented from dogs in Malaysia, occurrence of Babesia has been relatively little studied in their tick vectors. Accordingly, a total of 240 dogs and 140 Rhipicephalus sanguineus sensu lato (s.l.) (Acari: Ixodidae) ticks from Malaysia were molecularly screened for the presence of Babesia protozoa in the present study. Babesia gibsoni was only detected in ticks (1.4%), whereas Babesia vogeli was detected in both ticks (1...
May 12, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29762516/seasonal-patterns-in-the-prevalence-and-diversity-of-tick-borne-borrelia-burgdorferi-sensu-lato-anaplasma-phagocytophilum-and-rickettsia-spp-in-an-urban-temperate-forest-in-south-western-slovakia
#16
Michal Chvostáč, Eva Špitalská, Radovan Václav, Tatiana Vaculová, Lenka Minichová, Markéta Derdáková
In Europe, Ixodes ricinus is the most important vector of tick-borne zoonotic bacteria. It transmits spirochaetes from the Borrelia burgdorferi sensu lato complex, Anaplasma phagocytophilum and Rickettsia spp. Although spatial differences in the prevalence of tick-borne pathogens have been intensively studied, seasonal (within-year) fluctuations in the prevalence of these pathogens within sites are often overlooked. We analyzed the occurrence and seasonal dynamics of Ixodes ricinus in an urban forest in Bratislava, Slovakia...
May 15, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29760190/tick-borne-encephalitis-virus-nonstructural-protein-ns5-induces-rantes-expression-dependent-on-the-rna-dependent-rna-polymerase-activity
#17
Zifeng Zheng, Jieyu Yang, Xuan Jiang, Yalan Liu, Xiaowei Zhang, Mei Li, Mudan Zhang, Ming Fu, Kai Hu, Hanzhong Wang, Min-Hua Luo, Peng Gong, Qinxue Hu
Tick-borne encephalitis virus (TBEV) is one of the flaviviruses that targets the CNS and causes encephalitis in humans. The mechanism of TBEV that causes CNS destruction remains unclear. It has been reported that RANTES-mediated migration of human blood monocytes and T lymphocytes is specifically induced in the brain of mice infected with TBEV, which causes ensuing neuroinflammation and may contribute to brain destruction. However, the viral components responsible for RANTES induction and the underlying mechanisms remain to be fully addressed...
May 14, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29757511/ixodes-ricinus-parasitism-of-birds-increases-at-higher-winter-temperatures
#18
Robert W Furness, Euan N Furness
Increasing winter temperatures are expected to cause seasonal activity of Ixodes ricinus ticks to extend further into the winter. We caught birds during winter months (November to February) at a site in the west of Scotland over a period of 24 years (1993-1994 to 2016-2017) to quantify numbers of attached I. ricinus and to relate these to monthly mean temperature. No adult ticks were found on any of the 21,731 bird captures, but 946 larvae and nymphs were found, with ticks present in all winter months, on 16 different species of bird hosts...
June 2018: Journal of Vector Ecology: Journal of the Society for Vector Ecology
https://www.readbyqxmd.com/read/29753652/epidemiological-survey-of-a-cervine-theileria-in-wild-deer-questing-ticks-and-cattle-in-hokkaido-japan
#19
Shunya Shibata, Thillaiampalam Sivakumar, Ikuo Igarashi, Rika Umemiya-Shirafuji, Hisashi Inokuma, Shinya Fukumoto, Naoaki Yokoyama
Species of Theileria are tick-borne hemoprotozoan parasites of ruminants that can cause severe clinical disease. In this study, blood samples were obtained from 91 wild sika deer in various districts in Hokkaido, Japan. Samples were tested using a PCR assay designed to amplify a full-length major piroplasm surface protein (MPSP) gene of a cervine Theileria species, designated as Theileria sp. (sika 1). The amplicons of 57 out of 89 PCR-positive samples were cloned and sequenced. The sequences shared 99.1%-100% identity scores, indicating the MPSP gene of Theileria sp...
May 4, 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29752143/evaluation-of-the-vector-competence-of-six-ixodid-tick-species-for-rangelia-vitalii-apicomplexa-piroplasmorida-the-agent-of-canine-rangeliosis
#20
João F Soares, Francisco B Costa, Aline Girotto-Soares, Aleksandro S Da Silva, Raqueli T França, Sueli A Taniwaki, Bruno Dall'Agnol, José Reck, Mitika K Hagiwara, Marcelo B Labruna
Rangelia vitalii is the etiologic agent of canine rangeliosis, a severe piroplasmosis that affects domestic dogs in Brazil, Uruguay and Argentina. While R. vitalii is one of the most pathogenic tick-borne pathogens for dogs in the world, its tick vector has remained unknown. The present study evaluated the vector competence of Rhipicephalus sanguineus sensu lato (both tropical and temperate species), Amblyomma aureolatum, Amblyomma ovale, Amblyomma tigrinum, and Amblyomma sculptum for R. vitalii. These six tick species were selected for the study because they comprise the main tick species infesting dogs within the distribution area of canine rangeliosis in South America...
May 4, 2018: Ticks and Tick-borne Diseases
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