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https://www.readbyqxmd.com/read/28433789/macromolecular-crowding-for-tailoring-tissue-derived-fibrillated-matrices
#1
Valentina Magno, Jens Friedrichs, Heather M Weber, Marina C Prewitz, Mikhail V Tsurkan, Carsten Werner
Tissue-derived fibrillated matrices can be instrumental for the in vitro reconstitution of multiphasic extracellular microenvironments. However, despite of several advantages, the obtained scaffolds so far offer a rather narrow range of materials characteristics only. In this work, we demonstrate how macromolecular crowding (MMC) - the supplementation of matrix reconstitution media with synthetic or natural macromolecules in ways to create excluded volume effects (EVE) - can be employed for tailoring important structural and biophysical characteristics of kidney-derived fibrillated matrices...
April 19, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28433592/toxicity-assessment-of-multi-walled-carbon-nanotubes-on-cucurbita-pepo-l-under-well-watered-and-water-stressed-conditions
#2
Mehrnaz Hatami
The rapid increase in the production and application of various types of nanomaterials increases the possibility of their presence in total environment, which subsequently raises concerns about their potential threats to the first trophic level of organisms, specifically under varying environmental constraints. In this work, seeds of Cucurbita pepo L. were cultured in MS basal medium exposed to multi-walled carbon nanotubes (MWCNTs) at different concentrations (0, 125, 250, 500 and 1000μgmL(-1)) under two levels of water potential, well-watered (0MPa) and water stress (-1...
April 20, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28433514/cox-2-inhibition-potentiates-mouse-bone-marrow-stem-cell-engraftment-and-differentiation-mediated-wound-repair
#3
Ramasatyaveni Geesala, Neha R Dhoke, Amitava Das
BACKGROUND: Engraftment of transplanted stem cells is often limited by cytokine and noncytokine proinflammatory mediators at the injury site. We examined the role of Cyclooxygenase-2 (Cox-2)-induced cytokine-mediated inflammation on engraftment of transplanted bone marrow stem cells (BMSCs) at the wound site. METHODS: BMSCs isolated from male C57/BL6J mice were transplanted onto excisional splinting wounds in syngenic females in presence or absence of celecoxib, Cox-2 specific inhibitor (50 mg/kg, body weight [b wt]), to evaluate engraftment and wound closure...
April 19, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28432531/effectiveness-of-nanoencapsulated-methotrexate-against-osteosarcoma-cells-in-vitro-cytotoxicity-under-dynamic-conditions
#4
O Mitxelena-Iribarren, C L Hisey, M Errazquin-Irigoyen, Y González-Fernández, E Imbuluzqueta, M Mujika, M J Blanco-Prieto, S Arana
Cancer is a leading cause of mortality in the world, with osteosarcoma being one of the most common types among children between 1 and 14 years old. Current treatments including preoperative chemotherapy, surgery and postoperative chemotherapy produce several side effects with limited effectiveness. The use of lipid nanoparticles as biodegradable shells for controlled drug delivery shows promise as a more effective and targeted tumor treatment. However, in vitro validation of these vehicles is limited due to fluid stagnation in current techniques, in which nanoparticles sediment onto the bottom of the wells killing the cells by asphyxiation...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28431775/cinacalcet-hydrochloride
#5
Gamal A E Mostafa, Abdullah A Al-Badr
Cinacalcet hydrochloride is a calcimimetic agent that increases the sensitivity to the extracellular calcium of the calcium-sensing receptors of the parathyroid gland which regulates parathyroid hormone secretion. This comprehensive profile on cinacalcet hydrochloride starts with a description: nomenclature, formulae, chemical structure, elemental composition, and appearance. The uses and applications of the drug are included. The methods of preparation of cinacalcet hydrochloride are described and their respective schemes are outlined...
2017: Profiles of Drug Substances, Excipients, and related Methodology
https://www.readbyqxmd.com/read/28431388/retention-and-transport-of-graphene-oxide-in-water-saturated-limestone-media
#6
Shunan Dong, Yuanyuan Sun, Bin Gao, Xiaoqing Shi, Hongxia Xu, Jianfeng Wu, Jichun Wu
In this work, column experiments were conducted to investigate the transport characteristics of graphene oxide (GO) nanoparticles in limestone media under various electrolytes, solution pH, and humic acid (HA) concentration conditions. In the limestone media, GO exhibited relatively low mobility with the mass recovery rate lower than 65.2%, even when solution ionic strength was low. The presence of HA enhanced its mobility. In addition, the presence of S(2-), a divalent anion, also promoted GO transport in limestone media compared to Cl(-) under similar ionic strength conditions through neutralizing more positive charge and thus diminishing the cation bridging...
April 17, 2017: Chemosphere
https://www.readbyqxmd.com/read/28431214/interferon-%C3%AE-released-by-activated-cd8-t-lymphocytes-impairs-the-calcium-resorption-potential-of-osteoclasts-in-calcified-human-aortic-valves
#7
Edit Nagy, Yang Lei, Eduardo Martínez-Martínez, Simon C Body, Florian Schlotter, Michael Creager, Alexander Assmann, Kamal Khabbaz, Peter Libby, Göran K Hansson, Elena Aikawa
Calcium content in patients with calcific aortic valve disease (CAVD) correlates with the severity of stenosis. In CAVD, activated T lymphocytes localize with osteoclast regions; however, the functional consequences of this association remain unknown. We hypothesized that CD8(+) T cells modulate calcification in CAVD. Explanted CAVD valves (n = 52) dissected into noncalcified and calcified portions were subjected to mRNA extraction, real-time quantitative PCR, enzyme-linked immunosorbent assay, and immunohistochemical analyses...
April 18, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28430425/environmental-growth-of-enterococci-and-escherichia-coli-in-feedlot-catch-basins-and-a-constructed-wetland-in-the-absence-of-fecal-input
#8
Lisa Tymensen, Calvin W Booker, Sherry J Hannon, Shaun R Cook, Rahat Zaheer, Ron Read, Tim A McAllister
Population structures of fecal indicator bacteria (FIB) isolated from catch basins, a constructed wetland, and feces from a beef cattle feedlot were compared over a two-year period. Enterococcus hirae accounted for 92% of the fecal isolates, whereas secondary environments were characterized by greater relative abundance of environmentally adapted species including Enterococcus casseliflavus. While enterococci densities in the catch basins and wetland were similar under wet and drought conditions, E. hirae predominated during rainy periods while E...
April 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28430380/thermo-chromium-a-contactless-optical-molecular-thermometer
#9
Sven Otto, Norman Scholz, Thomas Behnke, Ute Resch-Genger, Katja Heinze
The unparalleled excited state potential energy landscape of the chromium(III)-based dye [1]3+ ([Cr(ddpd)2]3+; ddpd = N,N'-dimethyl-N,N'-dipyridin-2-yl-pyridin-2,6-diamine) enables a strong dual emission in the near infrared. The temperature dependence of this dual emission allows for the use of [1]3+ as an unprecedented molecular ratiometric thermometer in the 210 - 358 K temperature range in organic and in aqueous media. Incorporation of [1]3+ in biocompatible nanocarriers like 100 nm-sized polystyrene nanoparticles and solutol micelles provides nanodimensional thermometers operating under physiological conditions...
April 21, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28429706/direct-comparison-of-distinct-naive-pluripotent-states-in-human-embryonic-stem-cells
#10
S Warrier, M Van der Jeught, G Duggal, L Tilleman, E Sutherland, J Taelman, M Popovic, S Lierman, S Chuva De Sousa Lopes, A Van Soom, L Peelman, F Van Nieuwerburgh, D I M De Coninck, B Menten, P Mestdagh, J Van de Sompele, D Deforce, P De Sutter, B Heindryckx
Until recently, human embryonic stem cells (hESCs) were shown to exist in a state of primed pluripotency, while mouse embryonic stem cells (mESCs) display a naive or primed pluripotent state. Here we show the rapid conversion of in-house-derived primed hESCs on mouse embryonic feeder layer (MEF) to a naive state within 5-6 days in naive conversion media (NCM-MEF), 6-10 days in naive human stem cell media (NHSM-MEF) and 14-20 days using the reverse-toggle protocol (RT-MEF). We further observe enhanced unbiased lineage-specific differentiation potential of naive hESCs converted in NCM-MEF, however, all naive hESCs fail to differentiate towards functional cell types...
April 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28429578/soy-isoflavones-enhance-%C3%AE-defensin-synthesis-and-secretion-in-endometrial-epithelial-cells-with-exposure-to-tlr3-agonist-polyinosinic-polycytidylic-acid
#11
Yotesawee Srisomboon, Sutthasinee Poonyachoti, Chatsri Deachapunya
PROBLEM: β-defensins are important innate chemical barriers that protect the endometrium from pathogen invasion. The effects of soy isoflavones, genistein and daidzein, on the expression and secretion of porcine β-defensins (PBD) in endometrial epithelial cells were investigated under normal or poly I:C-stimulated conditions. METHOD OF STUDY: Primary cultured porcine endometrial epithelial (PE) cells were pretreated with genistein or daidzein followed by poly I:C inoculation...
April 21, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28429532/neu1-sialidase-interacts-with-perilipin-1-on-lipid-droplets-and-inhibits-lipolysis-in-3t3-l1-adipocytes
#12
Yujin Natori, Miwako Nasui, Fumiko Kihara-Negishi
Fatty acids are stored within adipocytes in lipid droplets (LDs) as triacylglycerol (TG), which is converted to free fatty acid (FFA) and glycerol via lipolysis. Increased plasma FFA levels in obesity are associated with several clinical conditions. We previously found that Neu1 activity is aberrant in the epididymal fat and liver of obese and diabetic mice. Here, we examined involvement of Neu1 in lipolysis in 3T3-L1 adipocytes. Small interfering RNA against Neu1 was introduced into adipocytes, and glycerol concentrations were measured in the culture medium...
April 21, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28429489/transcriptional-analysis-of-the-adaptation-of-ustilago-maydis-during-growth-under-nitrogen-fixation-conditions
#13
José Alejandro Sánchez-Arreguin, Miguel Angel Hernandez-Oñate, Claudia Geraldine León-Ramirez, José Ruiz-Herrera
Regulation of genes involved in nitrogen metabolism likely plays a role in the ability of fungi to exploit and survive under different environmental situations. To learn about the mechanism of adaptation of the biotrophic fungus Ustilago maydis from a medium containing a source of fixed nitrogen, to a medium depending on the ability to fix N2 by its bacterial endosymbiont, we explored gene expression profiles using RNA-Seq analyses under these two conditions. The differentially expressed (DE) fungal genes were analyzed, identifying 90 genes that were regulated 24 h after shifting the fungus to media lacking ammonium nitrate as a nitrogen source...
April 21, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28428025/adipokines-as-atherothrombotic-risk-factors-in-obese-subjects-associations-with-haemostatic-markers-and-common-carotid-wall%C3%A2-thickness
#14
É Csongrádi, M Káplár, B Nagy, C A Koch, A Juhász, L Bajnok, Z Varga, I Seres, Z Karányi, M T Magyar, L Oláh, A Facskó, J Kappelmayer, G Paragh
BACKGROUND AND AIMS: Some crucial associations between obesity-related altered adipokine levels and the main factors of atherosclerotic, atherothrombotic processes are not fully known. We analysed the relationships of classic adipokines, namely leptin, resistin, adiponectin, tumour necrosis factor-alpha (TNF-α), interleukin 6 (IL-6) with the markers of platelet activation, including mean platelet volume (MPV), platelet surface/soluble P-selectin, platelet-derived microparticles (PMPs), the parameters of coagulation abnormalities and common carotid intima-media thickness (IMT) in obese patients with or without atherosclerotic comorbidities in comparison to age- and sex-matched controls...
March 8, 2017: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/28427815/the-swedish-radiological-environmental-protection-regulations-applied-in-a-review-of-a-license-application-for-a-geological-repository-for-spent-nuclear-fuel
#15
Pål Andersson, Karolina Stark, Shulan Xu, Maria Nordén, Björn Dverstorp
For the first time, a system for specific consideration of radiological environmental protection has been applied in a major license application in Sweden. In 2011 the Swedish Nuclear Fuel & Waste Management Co. (SKB) submitted a license application for construction of a geological repository for spent nuclear fuel at the Forsmark site. The license application is supported by a post-closure safety assessment, which in accordance with regulatory requirements includes an assessment of environmental consequences...
April 17, 2017: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/28427684/rapid-and-high-capacity-ultrasonic-assisted-adsorption-of-ternary-toxic-anionic-dyes-onto-mof-5-activated-carbon-artificial-neural-networks-partial-least-squares-desirability-function-and-isotherm-and-kinetic-study
#16
Hanieh Askari, Mehrorang Ghaedi, Kheibar Dashtian, Mohammad Hossein Ahmadi Azghandi
The present paper focused on the ultrasonic assisted simultaneous removal of fast green (FG), eosin Y (EY) and quinine yellow (QY) from aqueous media following using MOF-5 as a metal organic framework and activated carbon hybrid (AC-MOF-5). The structure and morphology of AC-MOF-5 was identified by SEM, FTIR and XRD analysis. The interactive and main effects of variables such as pH, initial dyes concentration (mgL(-1)), adsorbent dosage (mg) and sonication time (min) on removal percentage were studied by central composite design (CCD), subsequent desirability function (DF) permit to achieved real variable experimental condition...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28427476/regeneration-of-full-thickness-skin-defects-by-differentiated-adipose-derived-stem-cells-into-fibroblast-like-cells-by-fibroblast-conditioned-medium
#17
Woojune Hur, Hoon Young Lee, Hye Sook Min, Maierdanjiang Wufuer, Chang-Won Lee, Ji An Hur, Sang Hyon Kim, Byeung Kyu Kim, Tae Hyun Choi
BACKGROUND: Fibroblasts are ubiquitous cells in the human body and are absolutely necessary for wound healing such as for injured skin. This role of fibroblasts was the reason why we aimed to differentiate human adipose-derived stem cells (hADSCs) into fibroblasts and to test their wound healing potency. Recent reports on hADSC-derived conditioned medium have indicated stimulation of collagen synthesis as well as migration of dermal fibroblasts in wound sites with these cells. Similarly, human fibroblast-derived conditioned medium (F-CM) was reported to contain a variety of factors known to be important for growth of skin...
April 20, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28426715/acute-hypoxia-influences-collagen-and-matrix-metalloproteinase-expression-by-human-keratoconus-cells-in-vitro
#18
Tina B McKay, Jesper Hjortdal, Shrestha Priyadarsini, Dimitrios Karamichos
Keratoconus (KC) is a progressive corneal ectasia linked to thinning of the central cornea. Hard contact lenses, rigid gas permeable lenses, and scleral lenses are the primary treatment modalities for early to mid- stages of KC to correct refractive error and astigmatism that develops as a result of an irregular corneal structure. These treatments are associated with significant drawbacks, including reduced availability of the tear film and oxygen to the corneal epithelium and stroma. However, it remains unknown whether hypoxia affects corneal integrity in the KC pathobiology...
2017: PloS One
https://www.readbyqxmd.com/read/28426371/optimization-of-preculture-conditions-to-maximize-the-in-vivo-performance-of-cell-seeded-engineered-intervertebral-discs
#19
John T Martin, Sarah E Gullbrand, Bhavana Mohanraj, Beth G Ashinsky, Dong Hwa Kim, Kensuke Ikuta, Dawn M Elliott, Lachlan J Smith, Robert L Mauck, Harvey E Smith
The development of engineered tissues has progressed over the past 20 years from in vitro characterization to in vivo implementation. For musculoskeletal tissue engineering in particular, the emphasis of many of these studies was to select conditions that maximized functional and compositional gains in vitro. However, the transition from the favorable in vitro culture environment to a less favorable in vivo environment has proven difficult, and, in many cases, engineered tissues do not retain their preimplantation phenotype after even short periods in vivo...
April 19, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28426331/effect-of-deletion-of-2-3-butanediol-dehydrogenase-gene-bdha-on-acetoin-production-of-bacillus-subtilis
#20
Junjiao Zhang, Xiangying Zhao, Jiaxiang Zhang, Chen Zhao, Jianjun Liu, Yanjun Tian, Liping Yang
The present work aims to block 2,3-butanediol synthesis in acetoin fermentation of Bacillus subtilis. First, we constructed a recombinant strain BS168D by deleting the 2,3-butanediol dehydrogenase gene bdhA of the B. subtilis168 and there was almost no 2,3-butanediol production in 20 g/L of glucose media. The acetoin yield of BS168D reached 6.61 g/L, which was about 1.5 times higher than that of the control B. subtilis168 (4.47 g/L). Then, when the glucose concentration was rised to 100 g/L, the acetoin yield reached 24...
April 20, 2017: Preparative Biochemistry & Biotechnology
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