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https://www.readbyqxmd.com/read/29656182/co-culture-of-ovarian-cancer-stem-like-cells-with-macrophages-induced-skov3-cells-stemness-via-il-8-stat3-signaling
#1
Yingxia Ning, Yinghong Cui, Xiang Li, Xiaocheng Cao, A Chen, Chang Xu, Jianguo Cao, Xin Luo
Among recent concepts in the cancer biology field, the tumor microenvironment is highly associated with cancer stem cells, and plays a key role in tumor progression. This study aimed to explore the mechanism that the stemness induction of SKOV3 cell line by macrophages derived from THP-1 cells, which was co-cultured with SKOV3-derived ovarian cancer stem-like cells (OCSLCs). Sphere formation, soft agar colony formation, and expression levels of CD133 and CD44 were assessed to reflect OCSLC properties. ELISA was used to evaluate secretion profile changes in macrophages co-cultured with or without SKOV3-derived OCSLCs...
April 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29656111/circulating-histocompatibility-antigen-hla-gene-products-may-help-differentiate-benign-from-malignant-indeterminate-pulmonary-lesions
#2
Smriti Kanangat, Christopher W Seder, Melissa R Pergande, Gabriela C Lobato, Cristina L Fhied, Maryam F Raouf, Michael J Liptay, Jeffrey A Borgia
BACKGROUND: This study explores the potential diagnostic utility of soluble Human Leukocyte Antigen (sHLA) molecules differentially released by lung adenocarcinoma and benign lung lesions. METHODS: Conditioned media from the NSCLC cell lines H358 and H1703 were immunoblotted for soluble isoforms of major histocompatibility complex (MHC) class I (ABC) and II (DRB1, DMB, and DQ) antigens. Sera from 25 patients with benign and 25 patients with malignant lesions were similarly evaluated to appraise the potential diagnostic value...
April 12, 2018: Human Immunology
https://www.readbyqxmd.com/read/29655629/intramuscular-vaccination-of-guinea-pigs-with-the-live-attenuated-human-herpes-simplex-vaccine-vc2-stimulates-a-transcriptional-profile-of-vaginal-th17-and-regulatory-tr1-responses
#3
Brent A Stanfield, Paul J F Rider, John Caskey, Fabio Del Piero, Konstantin G Kousoulas
Herpes simplex virus is a common causative agent of oral and genital diseases. Novel vaccines and therapeutics are needed to combat herpes infections especially after the failure of subunit vaccines in human clinical trials. We have shown that the live-attenuated HSV-1 VC2 vaccine strain is unable to establish latency in vaccinated animals and produces a robust immune response capable of completely protecting mice against lethal vaginal HSV-1 or HSV-2 infections. The guinea pig represents the best small animal model of genital HSV-2 disease...
April 11, 2018: Vaccine
https://www.readbyqxmd.com/read/29653286/stromal-cell-laden-3d-hydrogel-microwell-arrays-as-tumor-microenvironment-model-for-studying-stiffness-dependent-stromal-cell-cancer-interactions
#4
Xiaoshan Yue, Trung Dung Nguyen, Victoria Zellmer, Siyuan Zhang, Pinar Zorlutuna
Tumor properties such as growth and metastasis are dramatically dependent on the tumor microenvironment (TME). However, the diversity of the TME including the stiffness and the composition of the extracellular matrix (ECM), as well as the involvement of stromal cells, makes it extremely difficult to establish proper in vitro models for studying tumor growth and metastasis. In this research, we fabricated a stromal cell-laden microwell array system with tunable stiffness ranging from 200 Pa up to 3 kPa, which covers the stiffness range of normal and cancerous mammary tissues, to study the effect of ECM stiffness on stromal-cancer interaction...
April 3, 2018: Biomaterials
https://www.readbyqxmd.com/read/29650603/top-studies-relevant-to-primary-care-practice
#5
REVIEW
Danielle Perry, Michael R Kolber, Christina Korownyk, Adrienne J Lindblad, Jamil Ramji, Joey Ton, G Michael Allan
OBJECTIVE: To summarize 10 high-quality studies from 2017 that have strong relevance to primary care practice. QUALITY OF EVIDENCE: Study selection involved routine literature surveillance by a group of primary care health professionals. This included screening abstracts of important journals and Evidence Alerts, as well as searching the American College of Physicians Journal Club. MAIN MESSAGE: Topics of the 2017 articles include whether treating subclinical hypothyroidism improves outcomes or symptoms; whether evolocumab reduces cardiovascular disease as well as low-density lipoprotein levels; whether lifestyle interventions reduce medication use in patients with diabetes; whether vitamin D prevents cardiovascular disease, cancer, or upper respiratory tract infections; whether canagliflozin reduces clinical events in patients with diabetes; how corticosteroid injections affect knee osteoarthritis; whether drained abscesses benefit from antibiotic treatment; whether patients with diabetes benefit from bariatric surgery; whether exenatide reduces clinical events in patients with diabetes; and whether tympanostomy tubes affect outcomes in recurrent acute otitis media or chronic otitis media...
April 2018: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/29650023/sustained-impact-of-energy-dense-tv-and-online-food-advertising-on-children-s-dietary-intake-a-within-subject-randomised-crossover-counter-balanced-trial
#6
Jennifer Norman, Bridget Kelly, Anne-T McMahon, Emma Boyland, Louise A Baur, Kathy Chapman, Lesley King, Clare Hughes, Adrian Bauman
BACKGROUND: Policies restricting children's exposure to unhealthy food marketing have been impeded by the lack of evidence showing a direct link between food advertising exposure and children's energy intake and body weight. Food advertising exposure increases children's immediate food consumption, but whether this increased intake is compensated for at later eating occasions is not known; consequently the sustained effect on diets remains unclear. METHODS: We conducted a within-subject, randomised, crossover, counterbalanced study across four, six-day holiday camps in New South Wales, Australia between April 2016 and January 2017...
April 12, 2018: International Journal of Behavioral Nutrition and Physical Activity
https://www.readbyqxmd.com/read/29649441/small-intestine-microbiota-regulate-host-digestive-and-absorptive-adaptive-responses-to-dietary-lipids
#7
Kristina Martinez-Guryn, Nathaniel Hubert, Katya Frazier, Saskia Urlass, Mark W Musch, Patricia Ojeda, Joseph F Pierre, Jun Miyoshi, Timothy J Sontag, Candace M Cham, Catherine A Reardon, Vanessa Leone, Eugene B Chang
The gut microbiota play important roles in lipid metabolism and absorption. However, the contribution of the small bowel microbiota of mammals to these diet-microbe interactions remains unclear. We determine that germ-free (GF) mice are resistant to diet-induced obesity and malabsorb fat with specifically impaired lipid digestion and absorption within the small intestine. Small bowel microbes are essential for host adaptation to dietary lipid changes by regulating gut epithelial processes involved in their digestion and absorption...
April 11, 2018: Cell Host & Microbe
https://www.readbyqxmd.com/read/29648468/in-healthy-normotensive-subjects-age-and-blood-pressure-better-predict-subclinical-vascular-and-cardiac-organ-damage-than-atherosclerosis-biomarkers
#8
Alessandro Maloberti, Francesca Farina, Marco Carbonaro, Enrico Piccinelli, Ilaria Bassi, Francesco Pansera, Guido Grassi, Giuseppe Mancia, Paola Palestini, Cristina Giannattasio
PURPOSE: Only few studies evaluated biomarkers useful for defining the cardiovascular risk of a subject in a pre-clinical condition (i.e. healthy subjects). In this context we sought to determine the relationships of Plasminogen activator inhibitor type 1 (PAI-1), P-Selectin, Tissue Inhibitors Metalloproteinases type 1 (TIMP-1) and Cystatin-C with subclinical Target Organ Damage (TOD) in normotensive and normoglycemic subjects without known cardiovascular and kidney diseases. MATERIALS AND METHODS: 480 blood donors participated at the present analysis...
April 12, 2018: Blood Pressure
https://www.readbyqxmd.com/read/29645351/a-proteomics-approach-to-identify-candidate-proteins-secreted-by-m%C3%A3-ller-glia-that-protect-ganglion-cells-in-the-retina
#9
Noelia Ruzafa, Xandra Pereiro, Marlen F Lepper, Stefanie M Hauck, Elena Vecino
The retinal Müller glial cells, like other types of glial cells, can enhance the survival and activity of neurons, especially of retinal ganglion cells (RGCs), which are the neurons affected in diseases such as glaucoma, diabetes and retinal ischemia. It has been demonstrated that Müller glia release neurotrophic factors that support RGC survival, yet many of these factors remain to be elucidated. To define these neurotrophic factors, we adopted a quantitative proteomic approach aiming at identifying neuroprotective proteins...
April 12, 2018: Proteomics
https://www.readbyqxmd.com/read/29644883/in-vitro-toxicity-assessment-of-rivaroxaban-degradation-products-and-kinetic-evaluation-to-decay-process
#10
Nathalie R Wingert, Marcelo D Arbo, Gabriela Göethel, Bárbara da Costa, Louise F Altknecht, Solange C Garcia, Martin Steppe
Degradation kinetics of oral anticoagulant rivaroxaban (RIV) was assessed in acid and alkaline media and while exposed to UVC radiation. Among all stress conditions tested, kinetic degradation process was better described by a zero-order model. A stability indicating method was validated for the analysis of the anticoagulant RIV in tablets by high-performance liquid chromatography. Robustness was evaluated with a two-level Plackett-Burman experimental design. The effect of acute exposition of the human hepatoblastoma HepG2 cell line to RIV stressed samples (100 and 500 µM) was assessed through in vitro toxicity tests...
April 12, 2018: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/29644162/clinical-study-on-middle-ear-diseases-in-children-with-orofacial-clefts
#11
Raja Kalaiarasi, Kamran A Syed, Chellappa Vijayakumar, Ajoy M Varghese, Mary John
Introduction Orofacial clefts are associated with middle ear diseases, but the magnitude of this problem is not generally well appreciated. The aim of this study was to describe the middle ear findings and audiological profile in children with orofacial clefts. Materials and methods Children with orofacial clefts attending plastic surgery and otorhinolaryngology departments of a tertiary hospital over one-year duration were included in this study. Ninety-six children with orofacial clefts were identified. They were categorized age-wise as zero to five years, more than five years to 10 years, and more than 10 years to 15 years...
February 8, 2018: Curēus
https://www.readbyqxmd.com/read/29643466/etidronate-prevents-dystrophic-cardiac-calcification-by-inhibiting-macrophage-aggregation
#12
Carolin Bauer, Olivier le Saux, Viola Pomozi, Redouane Aherrahrou, Rene Kriesen, Stephanie Stölting, Annett Liebers, Thorsten Kessler, Heribert Schunkert, Jeanette Erdmann, Zouhair Aherrahrou
Cardiovascular calcification is associated with high risk of vascular disease. This involves macrophage infiltration of injured vascular tissue and osteoclast-related processes. Splenic monocytes from mice, that are predisposed (C3H) or resistant (B6) to calcification, were isolated and differentiated in vitro with M-CSF to generate macrophages, which aggregate to form multinucleated (MN) cells in the presence of RANKL. MN cell formation was significantly decreased in monocytes from resistant compared with calcifying mice...
April 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29643461/extending-a-gray-lattice-boltzmann-model-for-simulating-fluid-flow-in-multi-scale-porous-media
#13
Jiujiang Zhu, Jingsheng Ma
A gray lattice Boltzmann model has previously been developed by the authors of this article to simulate fluid flow in porous media that contain both resolved pores and grains as well as aggregates of unresolved smaller pores and grains. In this model, a single parameter is introduced to prescribe the amount of fluid to be bounced back at each aggregate cell. This model has been shown to recover Darcy-Brinkman flow but with effective viscosity and permeability correlated through the model parameter. In this paper, we prove that the model parameter relates to the fraction of the solid phase of a sub-pore system for a specific set of bounce-back conditions...
April 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29643433/neurofibromin-knockdown-in-glioma-cell-lines-is-associated-with-changes-in-cytokine-and-chemokine-secretion-in-vitro
#14
Matthew D Wood, Joydeep Mukherjee, Russell O Pieper
The neurofibromin-1 tumor suppressor gene (NF1) is altered in approximately 20% of sporadic glioblastoma (GBM) cases. NF1 deficient GBM frequently shows a mesenchymal gene expression signature, suggesting a relationship between NF1 status and the tumor microenvironment. To identify changes in the production of secreted cytokines/chemokines in NF1 deficient glioma, we applied cytokine arrays to conditioned media from a panel of three GBM cell lines after siRNA-mediated NF1 knockdown. We identified increased secretion of platelet-derived growth factor AA (PDGF-AA), chitinase-3-like protein 1 (CHI3L1), interleukin-8 (IL-8), and endoglin (ENG) in different subsets of these cell lines...
April 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29643170/treatment-options-in-idiopathic-subglottic-stenosis-protocol-for-a-prospective-international-multicentre-pragmatic-trial
#15
Alexander Gelbard, Yu Shyr, Lynne Berry, Alexander T Hillel, Dale C Ekbom, Eric S Edell, Jan L Kasperbauer, David G Lott, Donald T Donovan, C Gaelyn Garrett, Guri Sandhu, James J Daniero, James L Netterville, Josh S Schindler, Marshall E Smith, Paul C Bryson, Robert R Lorenz, David O Francis
INTRODUCTION: Idiopathic subglottic stenosis (iSGS) is an unexplained progressive obstruction of the upper airway that occurs almost exclusively in adult, Caucasian women. The disease is characterised by mucosal inflammation and localised fibrosis resulting in life-threatening blockage of the upper airway. Because of high recurrence rates, patients with iSGS will frequently require multiple procedures following their initial diagnosis. Both the disease and its therapies profoundly affect patients' ability to breathe, communicate and swallow...
April 10, 2018: BMJ Open
https://www.readbyqxmd.com/read/29643156/indecho-study-cohort-study-investigating-birth-size-childhood-growth-and-young-adult-cardiovascular-risk-factors-as-predictors-of-midlife-myocardial-structure-and-function-in-south-asians
#16
Senthil K Vasan, Ambuj Roy, Viji Thomson Samuel, Belavendra Antonisamy, Santosh K Bhargava, Anoop George Alex, Bhaskar Singh, Clive Osmond, Finney S Geethanjali, Fredrik Karpe, Harshpal Sachdev, Kanhaiya Agrawal, Lakshmy Ramakrishnan, Nikhil Tandon, Nihal Thomas, Prasanna S Premkumar, Prrathepa Asaithambi, Sneha F X Princy, Sikha Sinha, Thomas Vizhalil Paul, Dorairaj Prabhakaran, Caroline H D Fall
INTRODUCTION: South Asians have high rates of cardiovascular disease (CVD) and its risk factors (hypertension, diabetes, dyslipidaemia and central obesity). Left ventricular (LV) hypertrophy and dysfunction are features of these disorders and important predictors of CVD mortality. Lower birth and infant weight and greater childhood weight gain are associated with increased adult CVD mortality, but there are few data on their relationship to LV function. The IndEcho study will examine associations of birth size, growth during infancy, childhood and adolescence and CVD risk factors in young adulthood with midlife cardiac structure and function in South Asian Indians...
April 10, 2018: BMJ Open
https://www.readbyqxmd.com/read/29629366/comparing-the-cost-effectiveness-of-campaigns-delivered-via-various-combinations-of-television-and-online-media
#17
REVIEW
Vanessa Allom, Michelle Jongenelis, Terry Slevin, Stacey Keightley, Fiona Phillips, Sarah Beasley, Simone Pettigrew
Background: Reflecting the increasing prevalence of online media, many mass media health campaigns are now delivered using both television (TV) and online media formats. The aim of this study was to evaluate a smoking cessation mass media campaign according to the cost-effectiveness of the various combinations of TV and online media formats to inform future media buying decisions. Methods: A quasi-experimental interrupted time series approach was employed. The campaign was delivered in seven 1-week bursts using TV, online video (OV), or online display (OD) (e...
2018: Frontiers in Public Health
https://www.readbyqxmd.com/read/29627132/an-image-processing-approach-for-investigation-on-transport-of-iron-oxide-nanoparticles-fe-3-o-4-stabilized-with-poly-acrylic-acid-in-two-dimensional-porous-media
#18
M Golzar, M Azhdary Moghaddam, S F Saghravani, B Dahrazma
Iron oxide nanoparticles were stabilized using poly acrylic acid (PAA) to yield stabilized slurry of Iron oxide nanoparticles. A two-dimensional physical model filled by glass beads was used to study the fate and transport of the iron oxide nanoparticles stabilized with PAA in porous media under saturated, steady-state flow conditions. Transport data for a nonreactive tracer, slurry of iron oxide nanoparticles stabilized with PAA were collected under similar flow conditions. The results show that low concentration slurry of iron oxide nanoparticles stabilized with PAA can be transported like a tracer without significant retardation...
March 31, 2018: Journal of Contaminant Hydrology
https://www.readbyqxmd.com/read/29626380/the-arbuscular-mycorrhizal-fungus-rhizophagus-irregularis-uses-a-reductive-iron-assimilation-pathway-for-high-affinity-iron-uptake
#19
Elisabeth Tamayo, Simon A B Knight, Ascensión Valderas, Andrew Dancis, Nuria Ferrol
Arbuscular mycorrhizal (AM) fungi can improve iron (Fe) acquisition of their host plants. Here, we report a characterization of two components of the high-affinity reductive Fe uptake system of Rhizophagus irregularis, the ferric reductase (RiFRE1) and the high affinity Fe permeases (RiFTR1-2). In the extraradical mycelia (ERM), Fe deficiency induced activation of a plasma membrane-localized ferric reductase, an enzyme that reduces Fe(III) sources to the more soluble Fe(II). Yeast mutant complementation assays showed that RiFRE1 encodes a functional ferric reductase and RiFTR1 an iron permease...
April 6, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29626318/thermal-stability-and-conformation-of-dna-and-proteins-under-the-confined-condition-in-the-matrix-of-hydrogels
#20
Shu-Ichi Nakano, Daisuke Yamaguchi, Naoki Sugimoto
Spatially confined environments are seen in biological systems and in the fields of biotechnology and nanotechnology. The confinement restricts the conformational space of polymeric molecules and increasing the degree of molecular crowding. Here, we developed preparation methods for agarose and polyacrylamide gels applicable to UV spectroscopy that can evaluate the confinement effects on DNA and protein structures. Measurements of UV absorbance and CD spectra showed no significant effect of the confinement in the porous media of agarose gels on the base-pair stability of DNA polynucleotides [poly(dA)/poly(dT)] and oligonucleotides (hairpin, duplex, and triplex structures)...
April 6, 2018: Molecular Biology Reports
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