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https://www.readbyqxmd.com/read/29235233/sodium-4-mercaptophenolate-capped-cdse-zns-quantum-dots-as-a-fluorescent-probe-for-ph-detection-in-acidic-aqueous-media
#1
Hu Xu, Dong Li, Yun Zhao, Xiaomei Wang, Dan Li, Yuhong Wang
Development of the fluorescent pH detection method is promising due to the sensitivity, easy operation, and low-cost, etc. However, traditional organic fluorophores have still some disadvantages such as the tedious preparation and purification as well as low photostability and water solubility, which limits the rapid detection application. Semiconductor quantum dots (QDs) have recently risen to prominence as an alternative for organic fluorophores in fluorescence analysis by virtue of their convenient synthesis and superior optical properties...
December 12, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/29235057/design-and-assessment-of-a-dynamic-perfusion-bioreactor-for-large-bone-tissue-engineering-scaffolds
#2
Birru Bhaskar, Robert Owen, Hossein Bahmaee, Parcha Sreenivasa Rao, Gwendolen C Reilly
Bioreactors can be used to apply fluid flow in vitro to scaffolds to improve mass transport of media and apply mechanical forces to cells. In this study, we developed and tested an autoclavable, modular perfusion bioreactor suitable for large scaffolds. We investigated the effects of fluid flow induced shear stress (FFSS) on osteogenic differentiation of human embryonic stem cell-derived mesenchymal progenitors (hES-MP cells) cultured on large polyurethane (PU) scaffolds (30 mm diameter × 5 mm thickness) in osteogenesis induction media (OIM)...
December 12, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29234458/robust-genetic-transformation-of-sorghum-sorghum-bicolor-l-using-differentiating-embryogenic-callus-induced-from-immature-embryos
#3
Srinivas Belide, Thomas Vanhercke, James Robertson Petrie, Surinder Pal Singh
Background: Sorghum (Sorghum bicolor L.) is one of the world's most important cereal crops grown for multiple applications and has been identified as a potential biofuel crop. Despite several decades of study, sorghum has been widely considered as a recalcitrant major crop for transformation due to accumulation of phenolic compounds, lack of model genotypes, low regeneration frequency and loss of regeneration potential through sub-cultures. Among different explants used for genetic transformation of sorghum, immature embryos are ideal over other explants...
2017: Plant Methods
https://www.readbyqxmd.com/read/29233757/the-in-vitro-effect-of-antimicrobial-photodynamic-therapy-on-dental-microcosm-biofilms-from-partially-erupted-permanent-molars-a-pilot-study
#4
Fabiana Sodré de Oliveira, Thiago Cruvinel, Daniela Alejandra Cusicanqui Méndez, Evandro José Dionísio, Daniel Rios, Maria Aparecida Andrade Moreira Machado
BACKGROUND: The aPDT could enhance the prevention of dental caries lesions in pits and fissures of partially erupted molars, by killing microorganisms from complex dental biofilms. This pilot study aimed to evaluate the effect of the Antimicrobial Photodynamic Therapy (aPDT) on the viability of specific microorganism groups of dental microcosm biofilms from occlusal surfaces of first permanent molars in eruption. METHODS: Dental microcosm biofilms grown on bovine enamel blocks, from dental plaque collected on occlusal surfaces of a partially erupted lower right first permanent molar, with McBain medium plus 1% sucrose in anaerobic condition at 37°C for 72h...
December 9, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29233750/fibrin-hydrogels-as-a-xenofree-and-rapidly-degradable-support-for-transplantation-of-retinal-pigment-epithelium-monolayers
#5
Jarel K Gandhi, Zahid Manzar, Lori A Bachman, Cynthia Andrews-Pfannkoch, Travis Knudsen, Matthew Hill, Hannah Schmidt, Raymond Iezzi, Jose S Pulido, Alan D Marmorstein
Recent phase 1 trials of embryonic stem cell and induced pluripotent stem cell (iPSCs) derived RPE transplants for the treatment of macular degeneration have demonstrated the relative safety of this process. However, there is concern over clumping, thickening, folding, and wrinkling of the transplanted RPE. To deliver a flat RPE monolayer, current phase 1 trials are testing synthetic substrates for RPE transplantation. These substrates, however, cause localized inflammation and fibrosis in animal models due to long degradation times...
December 9, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29232632/inactivation-of-escherichia-coli-o157-h7-and-salmonella-deposited-on-gloves-in-a-liquid-state-and-subjected-to-drying-conditions
#6
Marilyn C Erickson, Jye-Yin Liao, Cathy C Webb, Mussie Y Habteselassie, Jennifer L Cannon
Gloves are worn by workers harvesting ready-to-eat produce as a deterrent for contaminating the produce with enteric pathogens that may reside on their hands. As fields are not sterile environments, the probability for gloves to become contaminated still exists and therefore it is critical to understand the conditions that affect the survival of pathogens on gloves. Both Escherichia coli O157:H7 and Salmonella deposited on glove surfaces in a liquid state survived longer when the pathogen had been suspended in lettuce sap than when suspended in water...
November 29, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29232104/the-effects-of-biological-fluids-on-colloidal-stability-and-sirna-delivery-of-a-ph-responsive-micellar-nanoparticle-delivery-system
#7
Dominic W Malcolm, Jomy J Varghese, Janet E Sorrells, Catherine E Ovitt, Danielle S W Benoit
Nanoparticles (NP) interact with complex protein milieus in biological fluids, and these interactions have profound effects on NP physicochemical properties and function. Surprisingly, most studies neglect the impact of these interactions, especially with respect to NP-mediated siRNA delivery. Here, the effects of serum on colloidal stability and siRNA delivery of a pH-responsive micellar NP delivery system were characterized. Results show cationic NP-siRNA complexes aggregate in  2% serum in buffer, but are stable in serum-free media...
December 12, 2017: ACS Nano
https://www.readbyqxmd.com/read/29232053/differential-diagnosis-of-otitis-media-with-effusion-using-label-free-raman-spectroscopy-a-pilot-study
#8
Rishikesh Pandey, Chi Zhang, Jeon Woong Kang, Parind M Desai, Ramachandra Rao Dasari, Ishan Barman, Tulio A Valdez
Otitis media with effusion (OME) is an important and common condition affecting hearing in pediatric patients characterized by the presence of fluid in the middle ear space. The fluid is normally described as serous or mucoid based on differences in the fluid viscosity. The differential diagnosis of two OMEs namely serous and mucoid is of significant clinical value because while the former is self-limiting, surgical procedure is commonly required for the latter. However, accurate identification of fluid types remains a challenging target unattainable with current clinical modalities due to unavailability of non-perturbative molecular tools...
December 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29231984/evidence-for-increased-chylomicron-remnants-in-rheumatoid-arthritis
#9
Benjamin Burggraaf, Deborah F van Breukelen-van der Stoep, Jendé van Zeben, Noelle van der Meulen, Gert-Jan M van de Geijn, Anho Liem, Pedro Valdivielso, José Rioja Villodres, José Ramírez-Bollero, Ellen van der Zwan, Manuel Castro Cabezas
BACKGROUND: Levels of apolipoprotein (apo) B48 may be increased in conditions associated with systemic inflammation and increased cardiovascular disease (CVD) risk like rheumatoid arthritis (RA). We aimed to evaluate apo B48 levels in patients with RA in relation to subclinical atherosclerosis. METHODS: Patients with RA (without CVD) and controls without RA but with high CVD risk (based on the presence of diabetes mellitus or a history of CVD) and healthy controls, were included in this cross-sectional study...
December 12, 2017: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29231944/stability-of-plant-virus-based-nanocarriers-in-gastrointestinal-fluids
#10
Alberto Berardi, David J Evans, Francesca Baldelli Bombelli, George P Lomonossoff
Cowpea mosaic virus (CPMV) is a plant virus which is being extensively investigated as a drug delivery and vaccine nanocarrier for parenteral administration. However, to date little is known about the suitability of plant-based nanocarriers for oral delivery. In this study, the colloidal (i.e. aggregation), physical (i.e. denaturation) and chemical (i.e. digestion of the polypeptides) stability of CPMV and its empty virus-like particles (eVLPs) in conditions resembling the gastrointestinal fluids were evaluated...
December 12, 2017: Nanoscale
https://www.readbyqxmd.com/read/29229727/high-salt-conditions-alter-transcription-of-helicobacter-pylori-genes-encoding-outer-membrane-proteins
#11
John T Loh, Amber C Beckett, Matthew B Scholz, Timothy L Cover
Helicobacter pylori infection and high dietary salt intake are risk factors for the development of gastric adenocarcinoma. One possible mechanism by which a high salt diet could influence gastric cancer risk is by modulating H. pylori gene expression. In this study, we utilized RNA-seq methodology to compare the transcriptional profiles of H. pylori grown in media containing different concentrations of sodium chloride. We identified 118 differentially expressed genes (65 upregulated and 53 downregulated in response to high salt conditions), including multiple members of 14 operons...
December 11, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29228269/electroactive-haloalkaliphiles-exhibit-exceptional-tolerance-to-free-ammonia
#12
Pablo Ledezma, Yang Lu, Stefano Freguia
Electrochemical activity in bacteria has been observed in numerous environments and conditions. However, enrichments in circumneutral freshwater media where acetate is the main electron donor seem to invariably lead to the dominance of Geobacter spp. Here we report on an electroactive bacterial consortium which was enriched on acetate as electron donor, but in a medium which reproduces hydrolysed urine (high pH, high salinity and high free ammonia). The consortium was found to be free of Geobacter species, whereas a previously undescribed community dominated by species closely related to Pseudomonas and Desulfuromonas was established...
December 4, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29228261/growth-and-carotenogenesis-in-rhodosporidium-diobovatum-imb-y-5023-effects-of-culture-medium-and-illumination-intensity
#13
Iuliia Ielchishcheva, Barbara Stachowiak, Artur Szwengiel, Anatoliy Bozhkov
The oleaginous yeast Rhodosporidium diobovatum is a poorly described producer of carotenoids and may be of interest in biotechnology. This study investigated the effects of culture medium and illumination on effective carotenoid production by R. diobovatum IMB Y-5023. Yeast was cultured on carrot, bran extract, and modified YM medium at illuminations in the 0-5,000 lx range. Biomass, total carotenoids, and their profile were assessed after cultivation. In order to samples comparison, cluster analysis (CA) and principle component analysis (PCA) were used to visualize the relationships between the variables and samples...
December 4, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29227975/high-phosphate-induced-calcification-of-vascular-smooth-muscle-cells-is-associated-with-the-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway
#14
Daohai Zhang, Xianjin Bi, Yong Liu, Yinghui Huang, Jiachuan Xiong, Xinli Xu, Tangli Xiao, Yanlin Yu, Wei Jiang, Yunjian Huang, Jingbo Zhang, Bo Zhang, Jinghong Zhao
BACKGROUND/AIMS: Hyperphosphatemia is one of the most notable features of chronic kidney disease (CKD). Numerous epidemiological and clinical studies have found that high serum phosphate concentrations are associated with calcification in the coronary arteries. However, the mechanisms underlying the vascular calcification induced by high phosphate have not been understood fully. METHODS: Vascular smooth muscle cells (VSMCs) were cultured in high-phosphate media to induce vascular calcification, which was detected by Alizarin red S staining...
December 8, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29227277/use-of-complementary-and-alternative-medicine-in-georgia
#15
I Nadareishvili, K Lunze, N Tabagari, A Beraia, G Pkhakadze
In Georgia, like in most countries globally, people commonly resort to complementary and alternative medicine (CAM). However, not much is known about CAM practices there. The aim of the study was to document common practices of CAM in Georgia and related patient attitudes. We collected data from peoples who commonly use CAM at 20 service provision centers in Georgia using cluster sampling from 300 patients. We admininstered a cross-sectional survey and conducted descriptive statistical analyses. People in Georgia use CAM either for prevention to improve general health (33%) or to treat chronic conditions (36%), spending about 25 Euros per month out of pocket...
November 2017: Georgian Medical News
https://www.readbyqxmd.com/read/29227088/host-guest-interaction-creates-hydrogen-evolution-electrocatalytic-active-sites-in-3d-transition-metal-intercalated-titanates
#16
Ruiqin Gao, Guangtao Yu, Wei Chen, Guo-Dong Li, Shuang Gao, Zengsong Zhang, Xiaopeng Shen, Xuri Huang, Xiaoxin Zou
The hydrogen evolution reaction (HER) is involved in energy-intensive water- and chlor-alkali electrolyzers, and thus, highly active and stable HER electrocatalysts in alkaline media are needed. Titanates, a family of representative two-dimensional materials with negatively charged main layers, are chemically and structurally stable under strongly basic conditions, but they have never been shown to have electrocatalytic activity for HER. Herein, we report that intercalating 3d metal cations, including Fe3+, Co2+, Ni2+ and Cu2+ ions, into the interlayer regions of titanates yields efficient and robust electrocatalysts for the alkaline HER...
December 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29226550/alpha-klotho-enrichment-in-induced-pluripotent-stem-cell-secretome-contributes-to-antioxidative-protection-in-acute-lung-injury
#17
Amiq Gazdhar, Priya Ravikumar, Johanne Pastor, Manfred Heller, Jianfeng Ye, Jianning Zhang, Orson W Moe, Thomas Geiser, Connie C W Hsia
Induced pluripotent stem cells (iPSCs) has been reported to alleviate organ injury, although the mechanisms of action remain unclear and administration of intact cells faces many limitations. We hypothesized that cell-free conditioned media (CM) containing the secretome of iPSCs possess antioxidative constituents that can alleviate pulmonary oxidant stress damage. We derived iPSCs from human dermal fibroblasts and harvested the CM. Addition of iPSC CM to cultured human alveolar type-1 epithelial cells mitigated hyperoxia-induced depletion of endogenous total antioxidant capacity (TAC) while tracheal instillation of iPSC CM into adult rat lungs enhanced hyperoxia-induced increase in TAC...
December 11, 2017: Stem Cells
https://www.readbyqxmd.com/read/29226464/place-of-death-in-the-snowy-monaro-region-of-new-south-wales-a-study-of-residents-who-died-of-a-condition-amenable-to-palliative-care
#18
Suzanne Rainsford, Nicholas J Glasgow, Rod D MacLeod, Teresa Neeman, Christine B Phillips, Robert B Wiles
OBJECTIVE: To describe the place of death of residents in a rural region of New South Wales. DESIGN: Cross-sectional quantitative study using death data collected from local funeral directors (in person and websites), residential aged-care facilities, one multipurpose heath service and obituary notices in the local media (newspapers/radio). SETTING: Snowy Monaro region (NSW Australia). PARTICIPANTS: Residents, with advanced frailty or one of 10 conditions amenable to palliative care, who died between 1 February 2015 and 31 May 2016...
December 11, 2017: Australian Journal of Rural Health
https://www.readbyqxmd.com/read/29226239/deconstructing-the-tissue-engineered-vascular-graft-evaluating-scaffold-pre-wetting-conditioned-media-incubation-and-determining-the-optimal-mononuclear-cell-source
#19
Cameron Best, Shuhei Tara, Matthew Wiet, James Reinhardt, Victoria Pepper, Matthew Ball, Tai Yi, Toshiharu Shinoka, Christopher Breuer
Stenosis limits widespread use of tissue-engineered vascular grafts (TEVGs), and bone marrow mononuclear cell (BM-MNC) seeding attenuates this complication. Yet seeding is a multistep process, and the singular effects of each component are unknown. We investigated which components of the clinical seeding protocol confer graft patency and sought to identify the optimal MNC source. Scaffolds composed of polyglycolic acid and ε-caprolactone/ι-lactic acid underwent conditioned media (CM) incubation (n = 25) and syngeneic BM-MNC (n = 9) or peripheral blood (PB)-MNC (n = 20) seeding...
2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29225709/immunomodulatory-effects-of-human-cryopreserved-viable-amniotic-membrane-in-a-pro-inflammatory-environment-in-vitro
#20
Claire E Witherel, Tony Yu, Mark Concannon, Will Dampier, Kara L Spiller
Introduction: Chronic wounds remain a major clinical challenge. Human cryopreserved viable amniotic membrane (hCVAM) is among the most successful therapies, but the mechanisms of action remain loosely defined. Because proper regulation of macrophage behavior is critical for wound healing with biomaterial therapies, we hypothesized that hCVAM would positively regulate macrophage behavior in vitro, and that soluble factors released from the hCVAM would be important for this effect. Materials and Methods: Primary human pro-inflammatory (M1) macrophages were seeded directly onto intact hCVAM or cultured in separation via transwell inserts (Soluble Factors) in the presence of pro-inflammatory stimuli (interferon-γ and lipopolysaccharide) to simulate the chronic wound environment...
October 2017: Cellular and Molecular Bioengineering
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