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https://www.readbyqxmd.com/read/28102936/unbiased-and-quantitative-proteomics-reveals-highly-increased-angiogenesis-induction-by-the-secretome-of-mesenchymal-stromal-cells-isolated-from-fetal-rather-than-adult-skin
#1
Massimiliano Gaetani, Cinzia Maria Chinnici, Anna Paola Carreca, Claudia Di Pasquale, Giandomenico Amico, Pier Giulio Conaldi
Scarless wound healing and functional regeneration are typical processes of the fetus, gradually lost during post-natal life, and maximally attributed to fetal skin tissue and induced by fetal skin fibroblasts. The latter have been successfully applied to post-natal wounds, with clear advantages compared with autologous dermis grafts or adult fibroblast applications. Our goal was to functionally identify and uncover key factors and mechanisms through the analysis of secretomes, the principal players in all cell therapies based on mesenchymal stromal cells (MSCs)...
January 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28102768/an-experimental-strategy-validated-to-design-cost-effective-culture-media-based-on-response-surface-methodology
#2
J L Navarrete-Bolaños, G P Téllez-Martínez, R Miranda-López, H Jiménez-Islas
For any fermentation process, the production cost depends on several factors, such as the genetics of the microorganism, the process condition and the culture medium composition. In this work, a guideline for the design of cost-efficient culture media using a sequential approach based on response surface methodology is described. The procedure was applied to analyze and optimize a culture medium of registered trademark and a base culture medium obtained as a results of the screening analysis from different culture media used to grow the same strain according to the literature...
January 19, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28102691/thermodynamic-and-kinetic-stabilities-of-g-quadruplexes-in-apolar-solvents
#3
Elham Fadaei, Miguel Martín-Arroyo, Mohsen Tafazzoli, David González-Rodríguez
In contrast to more polar media, G-quadruplex assembly reveals remarkably high thermodynamic and kinetic stability in toluene solutions. Depending on the substituents installed at the lipophilic guanosine, either a dodecamer or an octamer complex is formed in the presence of K(+) or Na(+) salts that resist conditions of high dilution and elevated temperatures without exhibiting significant dissociation. Moreover, kinetic exchange between complexed and uncomplexed G is slow enough in NMR to monitor G-quadruplex formation along a day time scale...
January 19, 2017: Organic Letters
https://www.readbyqxmd.com/read/28102364/indefinitely-stable-iron-iv-cage-complexes-formed-in-water-by-air-oxidation
#4
Stefania Tomyn, Sergii I Shylin, Dmytro Bykov, Vadim Ksenofontov, Elzbieta Gumienna-Kontecka, Volodymyr Bon, Igor O Fritsky
In nature, iron, the fourth most abundant element of the Earth's crust, occurs in its stable forms either as the native metal or in its compounds in the +2 or +3 (low-valent) oxidation states. High-valent iron (+4, +5, +6) compounds are not formed spontaneously at ambient conditions, and the ones obtained synthetically appear to be unstable in polar organic solvents, especially aqueous solutions, and this is what limits their studies and use. Here we describe unprecedented iron(IV) hexahydrazide clathrochelate complexes that are assembled in alkaline aqueous media from iron(III) salts, oxalodihydrazide and formaldehyde in the course of a metal-templated reaction accompanied by air oxidation...
January 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28101819/high-central-aortic-rather-than-brachial-blood-pressure-is-associated-with-carotid-wall-remodeling-and-increased-arterial-stiffness-in-childhood
#5
Gonzalo Peluso, Victoria García-Espinosa, Santiago Curcio, Marco Marota, Juan Castro, Pedro Chiesa, Gustavo Giachetto, Daniel Bia, Yanina Zócalo
INTRODUCTION: In adults, central blood pressure (cBP) is reported to associate target organ damages (TODs) rather than peripheral blood pressure (pBP). However, data regarding the association of pre-clinical TODs with cBP and pBP in pediatric populations are scarce. AIM: To evaluate in children and adolescents the importance of cBP and pBP levels, in terms of their association with hemodynamic and vascular changes. METHODS: 315 subjects [age (mean/range) 12/8-18 years] were included...
January 19, 2017: High Blood Pressure & Cardiovascular Prevention: the Official Journal of the Italian Society of Hypertension
https://www.readbyqxmd.com/read/28101416/light-field-otoscope-design-for-3d-in-vivo-imaging-of-the-middle-ear
#6
Noah Bedard, Timothy Shope, Alejandro Hoberman, Mary Ann Haralam, Nader Shaikh, Jelena Kovačević, Nikhil Balram, Ivana Tošić
We present a light field digital otoscope designed to measure three-dimensional shape of the tympanic membrane. This paper describes the optical and anatomical considerations we used to develop the prototype, along with the simulation and experimental measurements of vignetting, field curvature, and lateral resolution. Using an experimental evaluation procedure, we have determined depth accuracy and depth precision of our system to be 0.05-0.07 mm and 0.21-0.44 mm, respectively. To demonstrate the application of our light field otoscope, we present the first three-dimensional reconstructions of tympanic membranes in normal and otitis media conditions, acquired from children who participated in a feasibility study at the Children's Hospital of Pittsburgh of the University of Pittsburgh Medical Center...
January 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28101214/parp-1-may-be-involved-in-angiogenesis-in-epithelial-ovarian-cancer
#7
Wei Wei, Yan Li, Shuqing Lv, Cancan Zhang, Yongjie Tian
Poly (ADP-ribose) polymerase 1 (PARP-1) is involved in DNA repair and has been implicated in chemoresistance. The present study investigated whether PARP-1 promotes angiogenesis in ovarian cancer. PARP-1 and vascular endothelial growth factor A (VEGF-A) expression and CD34(+) microvascular density (MVD) were assessed using immunohistochemistry in 60 human epithelial ovarian cancer specimens. PARP-1 was stably knocked-down in SKOV3 cells using a specific small interfering RNA (siRNA); angiogenic capacity was assessed using the human umbilical vein endothelial cell (HUVEC) tubule formation assay; and PARP-1 and VEGF-A expression were examined by reverse transcription-quantitative polymerase chain reaction, western blotting and ELISA...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28100597/investigation-of-a-candida-guilliermondii-pseudo-outbreak-reveals-a-novel-source-of-laboratory-contamination
#8
James E Kirby, Westyn Branch-Elliman, Mary T LaSalvia, Lorinda Longhi, Matthew MacKechnie, Grigoriy Urman, Linda M Baldini, Fatima R Muriel, Bernadette Sullivan, David S Yassa, Howard S Gold, Trevor K Wagner, Daniel J Diekema, Sharon B Wright
Candida guilliermondii was isolated from sterile specimens with increasing frequency over a several-month period despite a paucity of clinical evidence suggesting true Candida infections. However, a healthcare-associated outbreak was strongly considered due to growth patterns in the microbiology laboratory more consistent with true infection than environmental contamination. Therefore, an extensive investigation was performed to identify its cause. With the exception of one case, patient clinical courses were not consistent with true invasive fungal infections...
January 18, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28100305/environment-directed-activation-the-escherichia-coli-flhdc-operon-by-transposons
#9
Zhongge Zhang, Chika Kukita, Mir Zafri Humayun, Milton H Saier
The flagellar system in E. coli K12 is expressed under the control of the flhDC-encoded master regulator, FlhDC. Transposition of insertion sequence (IS) elements to the upstream flhDC promoter region up-regulates transcription of this operon, resulting in more rapid motility. Wang and Wood (2011) provided evidence that insertion of IS5 into upstream activating sites occurs at higher rates in semisolid agar media in which swarming behavior is allowed as compared with liquid or solid media where swarming cannot occur...
January 17, 2017: Microbiology
https://www.readbyqxmd.com/read/28100297/transcriptional-analysis-of-mating-and-pre-infection-stages-of-the-anther-smut-microbotryum-lychnidis-dioicae
#10
Su San Toh, Zehua Chen, David J Schultz, Christina A Cuomo, Michael H Perlin
Microbotryum lychnidis-dioicae is an obligate biotrophic parasite of the wildflower species, Silene latifolia. This dikaryotic fungus, commonly known as an anther smut, requires that haploid, yeast-like sporidia of opposite mating types fuse and differentiate into dikaryotic hyphae that penetrate host tissue as part of the fungal lifecycle. Mating occurs under conditions of cool temperatures and limited nutrients. Further development requires host cues or chemical mimics, including a variety of lipids, e.g...
January 17, 2017: Microbiology
https://www.readbyqxmd.com/read/28099656/association-between-diabetes-and-tuberculosis-case-control-study
#11
Susan Martins Pereira, Gleide Santos de Araújo, Carlos Antônio de Souza Teles Santos, Maeli Gomes de Oliveira, Maurício Lima Barreto
OBJECTIVE: To test the association between diabetes and tuberculosis. METHODS: It is a case-control study, matched by age and sex. We included 323 new cases of tuberculosis with positive results for bacilloscopy. The controls were 323 respiratory symptomatic patients with negative bacilloscopy, from the same health services, such as: ambulatory cases from three referral hospitals and six basic health units responsible for the notifications of new cases of tuberculosis in Salvador, Bahia...
December 22, 2016: Revista de Saúde Pública
https://www.readbyqxmd.com/read/28098793/osteocyte-alterations-induce-osteoclastogenesis-in-an-in-vitro-model-of-gaucher-disease
#12
Constanza Bondar, Maximiliano Ormazabal, Andrea Crivaro, Malena Ferreyra-Compagnucci, María Victoria Delpino, Paula Adriana Rozenfeld, Juan Marcos Mucci
Gaucher disease (GD) is caused by mutations in the glucosylceramidase β (GBA 1) gene that confer a deficient level of activity of glucocerebrosidase (GCase). This deficiency leads to the accumulation of the glycolipid glucocerebroside in the lysosomes of cells, mainly in the monocyte/macrophage lineage. Its mildest form is Type I GD, characterized by non-neuronopathic involvement. Bone compromise is the most disabling aspect of the Gaucher disease. However, the pathophysiological aspects of skeletal alterations are not yet fully understood...
January 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28098294/co2-as-a-hydrogen-vector-transition-metal-diamine-catalysts-for-selective-hcooh-dehydrogenation
#13
Cornel Fink, Gábor Laurenczy
The homogeneous catalytic dehydrogenation of formic acid in aqueous solution provides an efficient in situ method for hydrogen production, under mild conditions, and at an adjustable rate. We synthesized a series of catalysts with the chemical formula [(Cp*)M(N-N')Cl] (M = Ir, Rh; Cp* = pentamethylcyclopentadienyl; N-N = bidentate chelating nitrogen donor ligands), which have been proven to be active in selective formic acid decomposition in aqueous media. The scope of the study was to examine the relationship between stability and activity of catalysts for formic acid dehydrogenation versus electronic and steric properties of selected ligands, following a bottom-up approach by increasing the complexity of the N,N'-ligands progressively...
January 18, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28097523/intravascular-ultrasound-characterization-of-a-tissue-engineered-vascular-graft-in-an-ovine-model
#14
Victoria K Pepper, Elizabeth S Clark, Cameron A Best, Ekene A Onwuka, Tadahisa Sugiura, Eric D Heuer, Lilamarie E Moko, Shinka Miyamoto, Hideki Miyachi, Darren P Berman, Sharon L Cheatham, Joanne L Chisolm, Toshiharu Shinoka, Christopher K Breuer, John P Cheatham
Patients who undergo implantation of a tissue-engineered vascular graft (TEVG) for congenital cardiac anomalies are monitored with echocardiography, followed by magnetic resonance imaging or angiography when indicated. While these methods provide data regarding the lumen, minimal information regarding neotissue formation is obtained. Intravascular ultrasound (IVUS) has previously been used in a variety of conditions to evaluate the vessel wall. The purpose of this study was to evaluate the utility of IVUS for evaluation of TEVGs in our ovine model...
January 17, 2017: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/28095968/catathrenia-nocturnal-groaning-a-social-media-survey-and-state-of-the-art-review
#15
Jose Alonso, Macario Camacho, Dinesh K Chhetri, Christian Guilleminault, Soroush Zaghi
STUDY OBJECTIVES: Catathrenia is an underrecognized nocturnal vocalization phenomenon that can be a source of perplexity to patients, bed partners, and medical providers. Catathrenia is distinct from both sleep talking (a parasomnia with loud talking during sleep) and snoring (noise due to vibration of upper airway soft tissues related to variations in airway resistance). The objective of this review is to provide an evidence-based resource to help the practitioner reliably evaluate and manage patients with this condition...
January 11, 2017: Journal of Clinical Sleep Medicine: JCSM: Official Publication of the American Academy of Sleep Medicine
https://www.readbyqxmd.com/read/28095523/effects-of-titanium-surface-microtopography-and-simvastatin-on-growth-and-osteogenic-differentiation-of-human-mesenchymal-stem-cells-in-estrogen-deprived-cell-culture
#16
Premjit Arpornmaeklong, Prisana Pripatnanont, Chonticha Chookiatsiri, Boonsin Tangtrakulwanich
PURPOSE: This study aimed to investigate the effects of titanium surface topography and simvastatin on growth and osteogenic differentiation of human bone marrow stromal cells (hBMSCs) in estrogen-deprived (ED) cell culture. MATERIALS AND METHODS: Human BMSCs were seeded on cell culture plates, smooth-surface titanium (Ti) disks, and sandblasted with large grits and acid etched (SLA)-surface Ti disks; and subsequently cultured in regular (fetal bovine serum [FBS]), ED, and ED-with 100 nM simvastatin (ED-SIM) culture media for 14 to 21 days...
January 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/28093281/kindlin-2-in-pancreatic-stellate-cells-promotes-the-progression-of-pancreatic-cancer
#17
Naoki Yoshida, Atsushi Masamune, Shin Hamada, Kazuhiro Kikuta, Tetsuya Takikawa, Fuyuhiko Motoi, Michiaki Unno, Tooru Shimosegawa
Pancreatic stellate cells (PSCs) play a pivotal role in pancreatic fibrosis associated with pancreatic ductal adenocarcinoma (PDAC). Kindlin-2 is a focal adhesion protein that regulates the activation of integrins. This study aimed to clarify the role of kindlin-2 in PSCs in pancreatic cancer. Kindlin-2 expression in 79 resected pancreatic cancer tissues was examined by immunohistochemical staining. Kindlin-2-knockdown immortalized human PSCs were established using small interfering RNA. Pancreatic cancer cells were treated with conditioned media of PSCs, and the cell proliferation and migration were examined...
January 13, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28093221/exposure-to-metal-oxide-nanoparticles-in-physiological-fluid-induced-synergistic-biological-effects-in-a-keratinocyte-model
#18
Deidre S Cathe, Jasmine N Whitaker, Emily K Breitner, Kristen K Comfort
Nanoparticles (NPs) possess distinctive physicochemical properties that in addition to differentiating them from their bulk counterparts can induce negative cellular consequences. Standard in vitro systems have served as the primary model for NP safety evaluations, but suffer from a lack physiological relevance. One way to overcome this limitation and evaluate NP characteristics under more accurate conditions is through the use of artificial physiological fluids, which mimic the composition of in vivo environments...
January 13, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28093072/in-vitro-effects-of-hyaluronic-acid-on-human-periodontal-ligament-cells
#19
Masako Fujioka-Kobayashi, Heinz-Dieter Müller, Andrea Mueller, Adrian Lussi, Anton Sculean, Patrick R Schmidlin, Richard J Miron
BACKGROUND: Hyaluronic acid (HA) has been reported to have a positive effect on periodontal wound healing following nonsurgical and surgical therapy. However, to date, a few basic in vitro studies have been reported to investigating the potential of HA on human periodontal ligament (PDL) cell regeneration. Therefore, the aim of this study was to investigate the effect of HA on PDL cell compatibility, proliferation, and differentiation in vitro. METHODS: Either non-cross-linked (HA_ncl) or cross-linked (HA_cl) HA was investigated...
January 16, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/28092842/blocking-tgf-%C3%AE-1-by-p17-peptides-attenuates-gastric-cancer-cell-induced-peritoneal-fibrosis-and-prevents-peritoneal-dissemination-in-vitro-and-in-vivo
#20
Zhi-Dong Lv, Wei-Jun Zhao, Li-Ying Jin, Wen-Juan Wang, Qian Dong, Na Li, Hui-Mian Xu, Hai-Bo Wang
Our previous study demonstrated that the peritoneal stroma environment favors proliferation of tumor cells by serving as a rich source of growth factors and chemokines known to be involved in tumor metastasis. In this study, we investigated the interaction between gastric cancer cells and peritoneal mesothelial cells, and determined the effects of TGF-β1 in this processing. Human peritoneal tissues and peritoneal wash fluid were obtained, which examined by hematoxylin and eosin staining or ELISA for measurements of TGF-β1 levels...
January 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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