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Peracetic acid sterilization

Alejandra Ramirez-Hernandez, Mindy M Brashears, Marcos X Sanchez-Plata
The poultry processing industry has been undergoing a series of changes as it modifies processing practices to comply with new performance standards for chicken parts and comminuted poultry products. The regulatory approach encourages the use of intervention strategies to prevent and control foodborne pathogens in poultry products and thus improve food safety and protect human health. The present studies were conducted to evaluate the efficacy of antimicrobial interventions for reducing Salmonella on inoculated chicken parts under simulated commercial processing conditions...
December 13, 2017: Journal of Food Protection
Cátia Fidalgo, Laura Iop, Manuela Sciro, Michael Harder, Dimosthenis Mavrilas, Sotirios Korossis, Andrea Bagno, Giorgio Palù, Paola Aguiari, Gino Gerosa
Decellularized xenogeneic scaffolds have shown promise to be employed as compatible and functional cardiovascular biomaterials. However, one of the main barriers to their clinical exploitation is the lack of appropriate sterilization procedures. This study investigated the efficiency of a two-step sterilization method, antibiotics/antimycotic (AA) cocktail and peracetic acid (PAA), on porcine and bovine decellularized pericardium. In order to assess the efficiency of the method, a sterilization assessment protocol was specifically designed, comprising: i) controlled contamination with a known amount of bacteria; ii) sterility test; iii) identification of contaminants through MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) mass spectrometry and iv) quantification by the Most Probable Number (MPN) method...
November 25, 2017: Acta Biomaterialia
Ozge Surucu, Serdar Abaci
Sterilization is the certain and absolute decontamination of microorganisms totally from all manner of alive and active species. Sterilization devices used in the sterilization processes are between laboratory and external patient application devices of biomedical device technology, and they are designed to remove equipments from germs. The application potential of hetarilazo indole based azo dyes in the biomedical sterilization are known and azo dyes come into prominence in this class because of simplicity of their synthesis and procurement of low-cost raw materials...
September 27, 2017: Journal of the Mechanical Behavior of Biomedical Materials
Leigh J Sowerby, Luke Rudmik
OBJECTIVE: Nasopharyngoscopes are an essential instrument to otolaryngologists; reprocessing them in a high-value manner is paramount. Although several different techniques for reprocessing exist, all methods yield similar effectiveness. Given equivalent effectiveness outcomes, a cost analysis of four nasopharyngoscope reprocessing techniques was performed. STUDY DESIGN: Cost-minimization analysis. METHODS: Four techniques were evaluated: 1) an automated reprocessor using peracetic acid (Steris System 1; Steris Canada Inc...
August 16, 2017: Laryngoscope
Abigail Phipps, Edward Vaynshteyn, John B Kowalski, Manh-Dan Ngo, Karen Merritt, Joel Osborne, Evangelia Chnari
Common terminal sterilization methods are known to alter the natural structure and properties of soft tissues. One approach to providing safe grafts with preserved biological properties is the combination of a validated chemical sterilization process followed by an aseptic packaging process. This combination of processes is an accepted method for production of sterile healthcare products as described in ANSI/AAMI ST67:2011. This article describes the validation of the peracetic acid and ethanol-based (PAAE) chemical sterilization process for allograft dermal tissues at the Musculoskeletal Transplant Foundation (MTF, Edison, NJ)...
December 2017: Cell and Tissue Banking
Alessandro F Pellegata, Marta Bottagisio, Federica Boschetti, Marco Ferroni, Monica Bortolin, Lorenzo Drago, Arianna B Lovati
In the last few years, the demand for tissue substitutes has increased and decellularized matrices has been widely proposed in the medical field to restore severe damages thanks to high biocompatibility and biomechanical properties similar to the native tissues. However, biological grafts represent a potential source of contamination and disease transmission; thus, there is the need to achieve acceptable levels of sterility. Several sterilization methods have been investigated with no consensus on the outcomes in terms of minimizing structural damages and preserving functional features of the decellularized matrix for transplantation in humans...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Ryan S Hennessy, Soumen Jana, Brandon J Tefft, Meghana R Helder, Melissa D Young, Rebecca R Hennessy, Nicholas J Stoyles, Amir Lerman
OBJECTIVE: The goal of this research project encompasses finding the most efficient and effective method of decellularized tissue sterilization. BACKGROUND: Aortic tissue grafts have been utilized to repair damaged or diseased valves. Although, the tissues for grafting are collected aseptically, it does not eradicate the risk of contamination nor disease transfer. Thus, sterilization of grafts is mandatory. Several techniques have been applied to sterilize grafts; however, each technique shows drawbacks...
February 2017: JACC. Basic to Translational Science
Georgi I Wassilew, Viktor Janz, Lisa Renner, Carsten Perka, Axel Pruss
The objective of the present study was to analyze the clinical and radiological results of periacetabular osteotomies (PAO) using Kirschner wire fixation and an allogeneic cancellous bone graft. This retrospective cohort study included 73 patients (85 PAOs). The allografts were processed from distal femur of cadaveric donors, defatted, sterilized with a peracetic-acid ethanol solution and freeze-dried. The clinical outcome, as measured by the Harris Hip Scores (HHS), the complication rate and the acetabular correction, as measured by radiological parameters, were compared...
December 2016: Cell and Tissue Banking
J Mohr, M Germain, M Winters, S Fraser, A Duong, A Garibaldi, N Simunovic, D Alsop, D Dao, R Bessemer, O R Ayeni
Musculoskeletal allografts are typically disinfected using antibiotics, irradiation or chemical methods but protocols vary significantly between tissue banks. It is likely that different disinfection protocols will not have the same level of microorganism kill; they may also have varying effects on the structural integrity of the tissue, which could lead to significant differences in terms of clinical outcome in recipients. Ideally, a disinfection protocol should achieve the greatest bioburden reduction with the lowest possible impact on tissue integrity...
December 2016: Cell and Tissue Banking
Jennifer H Edwards, Anthony Herbert, Gemma L Jones, Iain W Manfield, John Fisher, Eileen Ingham
Acellular xenogeneic tissues have the potential to provide 'off-the-shelf' grafts for anterior cruciate ligament (ACL) repair. To ensure that such grafts are sterile following packaging, it is desirable to use terminal sterilization methods. Here, the effects of gamma and electron beam irradiation on the biological and biomechanical properties of a previously developed acellular porcine superflexor tendon (pSFT) were investigated. Irradiation following treatment with peracetic acid was compared to peracetic acid treatment alone and the stability of grafts following long-term storage assessed...
November 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Beatriz Helena Dias Panariello, Fernanda Emiko Izumida, Eduardo Buozi Moffa, Ana Claudia Pavarina, Janaina Habib Jorge, Eunive Teresinha Giampaolo
PURPOSE: To investigate the efficacy of immersion and brushing with different cleansing agents in reducing the viability of multispecies biofilm on acrylic resins. METHODS: Lucitone 550 (L) and Tokuyama Rebase Fast II (T) specimens (10 x 2 mm) were prepared, sterilized, and inoculated with a suspension of Candida albicans, Candida glabrata, and Streptococcus mutans. Specimens were incubated for 48 hours at 37 degrees C for biofilm formation. Then, they were divided into groups (n = 12) and subjected to brushing or immersion for 10 seconds in distilled water (W), 0...
June 2016: American Journal of Dentistry
Wu Chenjiao, Zhang Hongyan, Gu Qing, Zhong Xiaoqi, Gu Liying, Fang Ying
Many high-level disinfectants have been used for disinfection of endoscopes such as 2% glutaraldehyde (GA), 0.55% ortho-phthalaldehyde (OPA), and peracetic acid (PAA). Both GA and OPA are widely used in disinfection of endoscopes and have been previously discussed, but there is little research on the practical use of PAA as an endoscope disinfectant. An experimental model of a flexible gastrointestinal endoscope being contaminated with 9 strains of microorganism was designed. After the cleaning and disinfecting procedure was completed, we evaluated the biocidal activity (850 ppm PAA, 2% GA, and 0...
March 2016: Gastroenterology Nursing: the Official Journal of the Society of Gastroenterology Nurses and Associates
Alexandra do Rosário Toniolo, Maíra Marques Ribeiro, Marina Ishii, Cely Barreto da Silva, Lycia Mara Jenné Mimica, Kazuko Uchikawa Graziano
A cross-sectional study was conducted to evaluate the effectiveness of manual and automated dialyzer reprocessing. Dialyzers were filled with fluid thioglycollate medium from blood and dialysate chambers after being reprocessed and chemically sterilized with 0.2% peracetic acid. They were incubated for 14 days at 35°C ± 2°C, and microbiologic analysis was performed. Microorganisms were identified in 3 of the 11 samples (27.3%) from the blood chambers: Sphingomonas paucimobilis (2/3) and Penicillium spp (1/3) and in 11 of the 11 samples (100%) from the dialysate chambers: S paucimobilis (7/11), Stenotrophomonas maltophilia (4/11), Pseudomonas aeruginosa (3/11), Candida spp (1/11), and Acinetobacter baumannii (1/11)...
June 1, 2016: American Journal of Infection Control
Vánia A Lacerda, Leandro O Pereira, Raphael Hirata JUNIOR, Cesar R Perez
PURPOSE: To evaluate the effectiveness of disinfection/sterilization methods and their effects on polishing capacity, micomorphology, and composition of two different composite fiishing and polishing instruments. METHODS: Two brands of finishing and polishing instruments (Jiffy and Optimize), were analyzed. For the antimicrobial test, 60 points (30 of each brand) were used for polishing composite restorations and submitted to three different groups of disinfection/sterilization methods: none (control), autoclaving, and immersion in peracetic acid for 60 minutes...
December 2015: American Journal of Dentistry
Jenna L Balestrini, Angela Liu, Ashley L Gard, Janet Huie, Kelly M S Blatt, Jonas Schwan, Liping Zhao, Tom J Broekelmann, Robert P Mecham, Elise C Wilcox, Laura E Niklason
Lung engineering is a potential alternative to transplantation for patients with end-stage pulmonary failure. Two challenges critical to the successful development of an engineered lung developed from a decellularized scaffold include (i) the suppression of resident infectious bioburden in the lung matrix, and (ii) the ability to sterilize decellularized tissues while preserving the essential biological and mechanical features intact. To date, the majority of lungs are sterilized using high concentrations of peracetic acid (PAA) resulting in extracellular matrix (ECM) depletion...
March 2016: Tissue Engineering. Part C, Methods
Mark J Leggett, J Spencer Schwarz, Peter A Burke, Gerald McDonnell, Stephen P Denyer, Jean-Yves Maillard
There is still great interest in controlling bacterial endospores. The use of chemical disinfectants and, notably, oxidizing agents to sterilize medical devices is increasing. With this in mind, hydrogen peroxide (H2O2) and peracetic acid (PAA) have been used in combination, but until now there has been no explanation for the observed increase in sporicidal activity. This study provides information on the mechanism of synergistic interaction of PAA and H2O2 against bacterial spores. We performed investigations of the efficacies of different combinations, including pretreatments with the two oxidizers, against wild-type spores and a range of spore mutants deficient in the spore coat or small acid-soluble spore proteins...
December 4, 2015: Applied and Environmental Microbiology
B Setlow, G Korza, K M S Blatt, J P Fey, P Setlow
AIMS: Determine how supercritical CO2 (scCO2 ) plus peracetic acid (PAA) inactivates Bacillus subtilis spores, factors important in spore resistance to scCO2 -PAA, and if spores inactivated by scCO2 -PAA are truly dead. METHODS AND RESULTS: Spores of wild-type B. subtilis and isogenic mutants lacking spore protective proteins were treated with scCO2 -PAA in liquid or dry at 35°C. Wild-type wet spores (aqueous suspension) were more susceptible than dry spores. Treated spores were examined for viability (and were truly dead), dipicolinic acid (DPA), mutations, permeability to nucleic acid stains, germination under different conditions, energy metabolism and outgrowth...
January 2016: Journal of Applied Microbiology
Joseph S Uzarski, Jimmy Su, Yan Xie, Zheng J Zhang, Heather H Ward, Angela Wandinger-Ness, William M Miller, Jason A Wertheim
This protocol details the generation of acellular, yet biofunctional, renal extracellular matrix (ECM) scaffolds that are useful as small-scale model substrates for organ-scale tissue development. Sprague Dawley rat kidneys are cannulated by inserting a catheter into the renal artery and perfused with a series of low-concentration detergents (Triton X-100 and sodium dodecyl sulfate (SDS)) over 26 hr to derive intact, whole-kidney scaffolds with intact perfusable vasculature, glomeruli, and renal tubules. Following decellularization, the renal scaffold is placed inside a custom-designed perfusion bioreactor vessel, and the catheterized renal artery is connected to a perfusion circuit consisting of: a peristaltic pump; tubing; and optional probes for pH, dissolved oxygen, and pressure...
2015: Journal of Visualized Experiments: JoVE
Xinglong Zheng, Junxi Xiang, Wanquan Wu, Xuemin Liu, Wenyan Liu, Yi Lü
OBJECTIVE: To develop a method for preparing a decellularized scaffold based on human liver tissue. METHODS: A surgical specimen of the left lateral lobe of the liver was obtained from a patients with hepatic hemangioma. The decellularization process was performed by repeated freezing-thawing, sequential perfusion with 0.01% SDS, 0.1% SDS and 1% Triton X-100 through the portal vein, and sterilization with peracetic acid. L-02 cells were then engrafted onto the decellularized liver scaffold...
July 2015: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Matheus Melo Pithon, Caio Souza Ferraz, Francine Cristina Silva Rosa, Luciano Pereira Rosa
OBJECTIVE: To investigate the effects of different sterilization/disinfection methods on the mechanical properties of orthodontic elastomeric chains. METHODS: Segments of elastomeric chains with 5 links each were sent for sterilization by cobalt 60 (Co60) (20 KGy) gamma ray technology. After the procedure, the elastomeric chains were contaminated with clinical samples of Streptococcus mutans. Subsequently, the elastomeric chains were submitted to sterilization/disinfection tests carried out by means of different methods, forming six study groups, as follows: Group 1 (control - without contamination), Group 2 (70°GL alcohol), Group 3 (autoclave), Group 4 (ultraviolet), Group 5 (peracetic acid) and Group 6 (glutaraldehyde)...
May 2015: Dental Press Journal of Orthodontics
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