Xueping Chen, Ke Fan, Jun Lu, Sheng Zhang, Jianhua Dong, Jisheng Qin, Weihua Fan, Yan Wang, Yiyuan Zhang, Huo Peng, Zhizhong Zhang, Zhiyong Sun, Chunlai Yu, Yucui Xiong, Yan Song, Qingqing Ye, Shiwen Mai, Yuanhua Wang, Qizheng Wang, Fengxiang Zhang, Xiaohui Wen, Tiancheng Zhou, Li Han, Mian Long, Guangjin Pan, Julian F Burke, Xiao Zhang
Somatic cell reprogramming generates induced pluripotent stem cells (iPSCs), which serve as a crucial source of seed cells for personalized disease modeling and treatment in regenerative medicine. However, the process of reprogramming often causes substantial lineage manipulations, thereby increasing cellular heterogeneity. As a consequence, the process of harvesting monoclonal iPSCs is labor-intensive and leads to decreased reproducibility. Here, we report the first in-house developed robotic platform that uses a pin-tip-based micro-structure to manipulate radial shear flow for automated monoclonal iPSC colony selection (~1 s) in a non-invasive and label-free manner, which includes tasks for somatic cell reprogramming culturing, medium changes; time-lapse-based high-content imaging; and iPSCs monoclonal colony detection, selection, and expansion...
2024: Research: a science partner journal