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https://www.readbyqxmd.com/read/28344594/articular-inflammation-induced-by-an-enzymatically-inactive-lys49-phospholipase-a2-activation-of-endogenous-phospholipases-contributes-to-the-pronociceptive-effect
#1
Renata Gonçalves Dias, Sandra Coccuzzo Sampaio, Morena Brazil Sant'Anna, Fernando Queiroz Cunha, José María Gutiérrez, Bruno Lomonte, Yara Cury, Gisele Picolo
BACKGROUND: Arthritis is a set of inflammatory conditions that induce aching, stiffness, swelling, pain and may cause functional disability with severe consequences to the patient's lives. These are multi-mediated pathologies that cannot be effectively protected and/or treated. Therefore, the aim of this study was to establish a new model of acute arthritis, using a Lys49-PLA2 (Bothrops asper myotoxin II; MT-II) to induce articular inflammation. METHODS: The articular inflammation was induced by MT-II (10 μg/joint) injection into the left tibio-tarsal or femoral-tibial-patellar joints...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28344413/evaluation-of-some-heavy-metals-residues-in-batteries-and-deep-litter-rearing-systems-in-japanese-quail-meat-and-offal-in-egypt
#2
Ali M Ahmed, Dalia M Hamed, Nagwa T Elsharawy
AIM: The main objectives of this study were for comparing the effect of batteries and deep litter rearing systems of domesticated Japanese quail, Coturnix coturnix japonica, on the concentration levels of cadmium, copper, lead, and zinc from the quail meat and offal in Ismailia, Egypt. MATERIALS AND METHODS: A total of 40 quail meat and their offal samples were randomly collected from two main quail rearing systems: Battery (Group I) and deep litter system (Group II) for determination of concentration levels of cadmium, copper, lead, and zinc...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28343244/a-new-reactive-1-8-naphthalimide-derivative-for-highly-selective-and-sensitive-detection-of-hg-2
#3
Feng Lv, Yufen Chen, Tengxuan Tang, Yuhua Chen, Dongmei Xu
A 1,8-naphthalimide derivative with a reactive aliphatic hydroxyl was designed and synthesized as a fluorescent probe. Its structure was characterized by IR, (1)H NMR, (13)C NMR, LC-MS and HPLC. The probe showed high selectivity and sensitivity to Hg(2+) over other metal ions such as Pb(2+), Na(+), K(+), Cd(2+), Cr(3+), Zn(2+), Cu(2+), Ni(2+), Ca(2+), Fe(3+), Fe(2+), Co(2+), Mn(2+) and Mg(2+) in MeCN/H2O (15/85, v/v). The increase in fluorescence intensity was linearly proportional to the concentration of Hg(2+) in the range of 18-40 μM with a detection limit of 1...
March 25, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28342973/effects-of-a-sustained-release-formulation-of-1-25-dihydroxyvitamin-d3-glycosides-for-milk-fever-prevention-on-serum-1-25-dihydroxyvitamin-d3-calcium-and-phosphorus-in-dairy-cows
#4
Heinrich Bachmann, Michael Lanz, Susanne Kehrle, Wolfgang Bittner, Annick Toggenburger, Georg A Mathis, Walter Rambeck
Milk fever (MF) is a metabolic disease in dairy cows around parturition. The clinical lead sign is muscular paresis leading in severe cases to paralysis of the affected animal. Multiparturient animals of high performing dairy breeds are most likely to be affected and have a high probability of recurrence. An acute drop in blood calcium levels causes the disease when the demand for calcium at the onset of lactation exceeds the ability to replete blood calcium levels through mobilization from bone and intestinal uptake...
March 22, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28340025/comparison-of-breast-cancer-tumor-marker-test-results-a-retrospective-analysis-of-paired-ca-15-3-and-ca-27-29-testing-at-a-national-reference-laboratory
#5
David Lin, Jonathan Genzen
No abstract text is available yet for this article.
March 1, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28339969/the-quality-of-eggs-organic-and-nutraceutical-vs-conventional-and-their-technological-properties
#6
Agnieszka Filipiak-Florkiewicz, Katarzyna Deren, Adam Florkiewicz, Kinga Topolska, Leslaw Juszczak, Ewa Cieslik
The aim of the study was to compare the quality of commercially available eggs: organic (attested organic farm) - OE, nutraceutical (enriched in n-3 fatty acids) - NE, vs. conventional (cage raising system) - CE and their technological (emulsifying) properties. Their basic chemical composition (i.e., moisture, protein, fat, ash) as well as mineral (Na, K, Ca, Mg, Zn, Fe, Mn, Cu) contents were assessed, and fatty acid profiles were determined. Next, the suitability of the eggs for mayonnaise production was assessed...
February 23, 2017: Poultry Science
https://www.readbyqxmd.com/read/28338481/clinical-use-and-optimal-cutoff-value-of-ca15-3-in-evaluation-of-adnexal-mass-retrospective-cohort-study-and-review-of-the-literature
#7
Lena Sagi-Dain, Ofer Lavie, Ron Auslander, Shlomi Sagi
OBJECTIVE: To estimate the diagnostic performance and reference values of serum cancer antigen (Ca)15-3 levels in the triage of adnexal masses. MATERIALS AND METHODS: This retrospective cohort study was carried out in 481 patients referred to the Gynecology Department at Carmel Medical Center due to adnexal mass between years 2005 and 2012. All patients underwent surgery with histopathologically confirmed diagnosis and routine preoperative measurements of serum Ca125 and Ca15-3...
March 23, 2017: American Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28337278/synthesis-of-huaicarbon-a-b-and-their-activating-effects-on-platelet-glycoprotein-vi-receptor-to-mediate-collagen-induced-platelet-aggregation
#8
Hongli Yu, Yeqing Chen, Hao Wu, Kuilong Wang, Liping Liu, Xingde Zhang
Quercetin and rhamnose were efficiently converted into huaicarbon A/B by heating at 250°C for 10-15 min or at 200°C for 25-30 min. With the optimum molar ratio of quercetin/rhamnose (1:3), huaicarbon A and B yields reached 25% and 16% respectively after heating at 250°C, with 55% quercetin conversion. Huaicarbon A/B both promoted washed platelet aggregation dose-dependently, which was antagonized by an inhibitor of glycoprotein VI (GPVI) receptor. Similarly, they both promoted collagen-induced platelet aggregation in platelet-rich plasma in dose-dependent manners...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28334414/association-of-a-haplotype-in-the-nr3c2-gene-encoding-the-mineralocorticoid-receptor-with-chronic-central-serous-chorioretinopathy
#9
Elon H C van Dijk, Rosa L Schellevis, Maaike G J M van Bergen, Myrte B Breukink, Lebriz Altay, Paula Scholz, Sascha Fauser, Onno C Meijer, Carel B Hoyng, Anneke I den Hollander, Camiel J F Boon, Eiko K de Jong
Importance: Chronic central serous chorioretinopathy (cCSC) is a chorioretinal disease with unknown disease etiology. The glucocorticoid receptor and the mineralocorticoid receptor, 2 glucocorticoid-binding receptors, might be involved in the pathogenesis of cCSC. Objective: To assess the association of functional variants and haplotypes in the glucocorticoid receptor (NR3C1) and mineralocorticoid receptor (NR3C2) genes with cCSC. Design, Setting, and Participants: In this case-control genetic association study, 336 patients with cCSC and 1314 unaffected controls, collected at 3 university medical centers from September 1, 2009, to May 1, 2016, underwent KASP genotyping for selected variants in NR3C1 (rs56149945, rs41423247, and rs6198) and NR3C2 (rs2070951 and rs5522)...
March 23, 2017: JAMA Ophthalmology
https://www.readbyqxmd.com/read/28332071/the-nutritive-value-of-marula-sclerocarya-birrea-seed-cake-for-broiler-chickens-nutritional-composition-performance-carcass-characteristics-and-oxidative-and-mycotoxin-status
#10
Doctor Mziwenkosi Nhlanhla Mthiyane, Bhekumusa Sabelo Mhlanga
This study was aimed at investigating the nutritive value of marula seed cake (MSC) as an alternative protein source for broilers. In a completely randomised design involving six replicate pens of five chickens assigned to each of five treatments, body weight gain (BWG), feed intake (FI), feed conversion efficiency (FCE) and carcass characteristics were measured in an experiment in which 150 28-day-old broilers were fed maize-based diets containing, respectively, 0, 5, 10, 15 and 20% MSC at finisher phase. The results showed MSC to be remarkably high in CP (470...
March 22, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28330908/the-effect-of-dairy-intake-on-bone-mass-and-body-composition-in-early-pubertal-girls-and-boys-a-randomized-controlled-trial
#11
Kara A Vogel, Berdine R Martin, Linda D McCabe, Munro Peacock, Stuart J Warden, George P McCabe, Connie M Weaver
Background: Calcium retention increases with increasing body mass index (BMI) on recommended calcium intakes. Dairy foods are an excellent source of essential nutrients that are needed to increase bone mineral content (BMC) and potentially decrease fracture.Objective: We compared children who were overweight with children who were healthy weight for the accrual of bone mass in response to an extra 3 servings dairy/d compared with usual intake.Design: Participants were 240 healthy boys and girls (64%), aged 8-15...
March 22, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28330241/purification-characterization-gene-cloning-and-expression-of-gh-10-xylanase-penicillium-citrinum-isolate-hzn13
#12
Zabin K Bagewadi, Sikandar I Mulla, Harichandra Z Ninnekar
An extracellular thermostable xylanase (Xyl-IIb) produced by Penicillium citrinum isolate HZN13 was purified to homogeneity using DEAE-Sepharose, Sephadex G-100 and Bio-Gel P-60 chromatography with specific activity of 6272.7 U/mg and 19.6-fold purification. The purification revealed the occurrence of multiple forms of xylanases (Xyl-I, Xyl-IIa, Xyl-IIb and Xyl-III). The molecular mass of highly purified Xyl-IIb was ~31 kDa with SDS-PAGE. The enzyme was cellulase-free, thermostable (55-75 °C) and acidophilic (3...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329313/single-coronary-artery-and-neonatal-arterial-switch-operation-early-and-long-term-outcomes%C3%A2
#13
Sébastien Gerelli, Margaux Pontailler, Bruno Rochas, Emanuela Angeli, Mathieu Van Steenberghe, Damien Bonnet, Pascal Vouhé, Olivier Raisky
OBJECTIVES: The presence of single coronary artery (CA) in the arterial switch operation (ASO) for neonatal treatment with transposition of the great arteries (TGA) has been reported to be an independent risk factor for early death after surgical repair and late reintervention. The study objective was to evaluate the mortality and the CA stenosis risk at early and long term in neonatal ASO for TGA and single CA. METHODS: Between January 1987 and January 2010, 979 neonates underwent an ASO, of which 73 had a single CA (7...
March 7, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28325704/the-efficiency-of-home-composting-programmes-and-compost-quality
#14
M A Vázquez, M Soto
The efficiency of home composting programmes and the quality of the produced compost was evaluated in eight rural areas carrying out home composting programmes (up to 880 composting bins) for all household biowaste including meat and fish leftovers. Efficiency was analysed in terms of reduction of organic waste collected by the municipal services. An efficiency of 77% on average was obtained, corresponding to a composting rate of 126kg/person·year of biowaste (or 380kg/composter·year). Compost quality was determined for a total of 90 composting bins...
March 18, 2017: Waste Management
https://www.readbyqxmd.com/read/28324920/an-open-label-randomized-parallel-phase-ii-trial-to-evaluate-the-efficacy-and-safety-of-a-cremophor-free-polymeric-micelle-formulation-of-paclitaxel-as-first-line-treatment-for-ovarian-cancer-a-korean-gynecologic-oncology-group-study-kgog-3021
#15
Shin-Wha Lee, Yong-Man Kim, Chi Heum Cho, Young Tae Kim, Seok Mo Kim, Soo Young Hur, Jae-Hoon Kim, Byoung-Gie Kim, Seung-Cheol Kim, Hee-Sug Ryu, Soon Beom Kang
Purpose: Genexol-PM is a biodegradable cremophor EL-free polymeric micelle formulation of paclitaxel. Here we compared efficacy and safety of Genexol-PM plus carboplatin vs. Genexol plus carboplatin for ovarian cancer treatment. Materials and Methods: In this multicenter, randomized, phase II study, patients with FIGO IC-IV epithelial ovarian cancer were randomly assigned (1:1) to receive Genexol-PM 260 mg/m2 or Genexol 175 mg/m2 with 5 AUC carboplatin every three weeks (6 cycles)...
March 21, 2017: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/28317895/upregulation-of-skeletal-muscle-pgc-1%C3%AE-through-the-elevation-of-cyclic-amp-levels-by-cyanidin-3-glucoside-enhances-exercise-performance
#16
Toshiya Matsukawa, Hideko Motojima, Yuki Sato, Shinya Takahashi, Myra O Villareal, Hiroko Isoda
Regular exercise and physical training enhance physiological capacity and improve metabolic diseases. Skeletal muscles require peroxisome proliferator-activated receptor-gamma coactivator-1α (PGC-1α) in the process of their adaptation to exercise owing to PGC-1α's ability to regulate mitochondrial biogenesis, angiogenesis, and oxidative metabolism. Cyanidin-3-glucoside (Cy3G) is a natural polyphenol and a nutraceutical factor, which has several beneficial effects on human health. Here, the effect of Cy3G on exercise performance and the underlying mechanisms involved were investigated...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28314621/docosahexaenoic-acid-dha-an-essential-nutrient-and-a-nutraceutical-for-brain-health-and-diseases
#17
REVIEW
Grace Y Sun, Agnes Simonyi, Kevin L Fritsche, Dennis Y Chuang, Mark Hannink, Zezong Gu, C Michael Greenlief, Jeffrey K Yao, James C Lee, David Q Beversdorf
Docosahexaenoic acid (DHA), a polyunsaturated fatty acid (PUFA) enriched in phospholipids in the brain and retina, is known to play multi-functional roles in brain health and diseases. While arachidonic acid (AA) is released from membrane phospholipids by cytosolic phospholipase A2 (cPLA2), DHA is linked to action of the Ca(2+)-independent iPLA2. DHA undergoes enzymatic conversion by 15-lipoxygenase (Alox 15) to form oxylipins including resolvins and neuroprotectins, which are powerful lipid mediators. DHA can also undergo non-enzymatic conversion by reacting with oxygen free radicals (ROS), which cause the production of 4-hydoxyhexenal (4-HHE), an aldehyde derivative which can form adducts with DNA, proteins and lipids...
March 10, 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/28297314/radiation-doses-and-estimated-risk-from-angiographic-projections-during-coronary-angiography-performed-using-novel-flat-detector
#18
Anna Varghese, Roshan S Livingstone, Lijo Varghese, Parveen Kumar, Sirish Chandra Srinath, Oommen K George, Paul V George
Coronary angiography (CA) procedure uses various angiographic projections to elicit detailed information of the coronary arteries with some steep projections involving high radiation dose to patients. This study intends to evaluate radiation doses and estimated risk from angiographic projections during CA procedure performed using novel flat detector (FD) system with improved image processing and noise reduction techniques. Real-time monitoring of radiation doses using kerma-area product (KAP) meter was performed for 140 patients using Philips Clarity FD system...
May 2016: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/28290327/effect-of-high-hydrostatic-pressure-processing-on-the-background-microbial-loads-and-quality-of-cantaloupe-puree
#19
Sudarsan Mukhopadhyay, Kimberly Sokorai, Dike Ukuku, Xuetong Fan, Vijay Juneja
The objective of this study was to investigate and evaluate the effects of high hydrostatic pressure (HHP) applied to cantaloupe puree (CP) on microbial loads and product quality during storage for 10days at 4°C. Freshly prepared, double sealed and double bagged CP (ca. 5g) was pressure treated at 300, 400 and 500MPa at 8°C and 15°C for 5min. Microflora populations, soluble solid content, pH, color, antioxidant activity, appearance and aroma were measured at 1, 6, and 10d of storage. Results showed that high pressure treatment of 300MPa (8°C and 15°C) resulted in reduction of total aerobic plate count from 3...
January 2017: Food Research International
https://www.readbyqxmd.com/read/28288347/exploration-of-the-metal-coordination-region-of-concanavalin-a-for-its-interaction-with-human-norovirus
#20
Duwoon Kim, Hee-Min Lee, Kyung-Seo Oh, Ah Young Ki, Rachael A Protzman, Dongkyun Kim, Jong-Soon Choi, Min Ji Kim, Sung Hyun Kim, Bipin Vaidya, Seung Jae Lee, Joseph Kwon
Rapid methods for the detection and clinical treatment of human norovirus (HuNoV) are needed to control foodborne disease outbreaks, but reliable techniques that are fast and sensitive enough to detect small amounts of HuNoV in food and aquatic environments are not yet available. We explore the interactions between HuNoV and concanavalin A (Con A), which could facilitate the development of a sensitive detection tool for HuNoV. Biophysical studies including hydrogen/deuterium exchange (HDX) mass spectrometry and surface plasmon resonance (SPR) revealed that when the metal coordinated region of Con A, which spans Asp16 to His24, is converted to nine alanine residues (mCon A(MCR)), the affinity for HuNoV (GII...
March 6, 2017: Biomaterials
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