keyword
MENU ▼
Read by QxMD icon Read
search

Live cell imaging

keyword
https://www.readbyqxmd.com/read/28727813/label-free-three-dimensional-imaging-of-caenorhabditis-elegans-with-visible-optical-coherence-microscopy
#1
Séverine Coquoz, Paul J Marchand, Arno Bouwens, Laurent Mouchiroud, Vincenzo Sorrentino, Daniel Szlag, Johan Auwerx, Theo Lasser
Fast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput in vivo time-lapse studies of the anatomy of Caenorhabditis elegans, one of the most commonly used model organisms in biomedical research. Despite the needs, methods combining all these characteristics have been lacking. Here, we present label-free imaging of live Caenorhabditis elegans with three-dimensional sub-micrometer resolution using visible optical coherence microscopy (visOCM). visOCM is a versatile optical imaging method which we introduced recently for tomography of cell cultures and tissue samples...
2017: PloS One
https://www.readbyqxmd.com/read/28727799/atlantic-salmon-cardiac-primary-cultures-an-in-vitro-model-to-study-viral-host-pathogen-interactions-and-pathogenesis
#2
Patricia A Noguera, Bianka Grunow, Matthias Klinger, Katherine Lester, Bertrand Collet, Jorge Del-Pozo
Development of Salmon Cardiac Primary Cultures (SCPCs) from Atlantic salmon pre-hatch embryos and their application as in vitro model for cardiotropic viral infection research are described. Producing SCPCs requires plating of trypsin dissociated embryos with subsequent targeted harvest from 24h up to 3 weeks, of relevant tissues after visual identification. SCPCs are then transferred individually to chambered wells for culture in isolation, with incubation at 15-22°. SCPCs production efficiency was not influenced by embryo's origin (0...
2017: PloS One
https://www.readbyqxmd.com/read/28726863/a-near-infrared-fluorescence-off-on-probe-for-sensitive-imaging-of-hydrogen-polysulfides-in-living-cells-and-mice-in-vivo
#3
Yu Fang, Wei Chen, Wen Shi, Hongyu Li, Ming Xian, Huimin Ma
A new hemicyanine-based near-infrared fluorescence off-on probe with phenyl 2-(benzoylthio)benzoate as the recognition moiety to trap hydrogen polysulfides (H2Sn, n > 1) is developed, and is used for the sensitive imaging of H2Sn in cells and mice in vivo.
July 20, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28726673/off-on-fluorescence-monitoring-of-intracellular-ag-in-single-living-cells-using-an-ag-responsive-probe
#4
Jian Lv, Li-Jun Zhao, Ruo-Can Qian, Yi-Tao Long
Detection of silver ions (Ag+) in living cells has becoming more and more attractive due to the important biological impact of Ag+ on cellular functions. Here, we put forward a new approach to realize the in situ fluorescence imaging and detection of Ag+ in single cells via an ultrasensitive Ag+-responsive probe, 3', 6'-bis (diethylamino)-2-(2-iodoethyl) spiro[isoindoline-1, 9'-xanthen]-3-one (BDISIX). In the presence of Ag+, the fluorescence of the probe can be turned "on", generating strong red fluorescence...
July 20, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28726636/live-cell-visualization-of-gasdermin-d-driven-pyroptotic-cell-death
#5
Joseph K Rathkey, Bryan L Benson, Steven M Chirieleison, Jie Yang, Tsan S Xiao, George R Dubyak, Alex Y Huang, Derek W Abbott
Pyroptosis is a form of cell death important in defenses against pathogens, but which can also result in a potent and sometimes pathological inflammatory response. During pyroptosis, gasdermin D (GSDMD), the pore-forming effector protein, is cleaved, forms oligomers, and inserts into the membranes of the cell, resulting in rapid cell death. However, the potent cell death induction caused by GSDMD has complicated our ability to understand the biology of this protein. Studies aimed at visualizing GSDMD have relied on expression of GSDMD fragments in epithelial cell lines that naturally lack GSDMD expression and also lack the proteases necessary to cleave GSDMD...
July 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28724529/coactivators-and-general-transcription-factors-have-two-distinct-dynamic-populations-dependent-on%C3%A2-transcription
#6
Nikolaos Vosnakis, Marc Koch, Elisabeth Scheer, Pascal Kessler, Yves Mély, Pascal Didier, László Tora
SAGA and ATAC are two distinct chromatin modifying co-activator complexes with distinct enzymatic activities involved in RNA polymerase II (Pol II) transcription regulation. To investigate the mobility of co-activator complexes and general transcription factors in live-cell nuclei, we performed imaging experiments based on photobleaching. SAGA and ATAC, but also two general transcription factors (TFIID and TFIIB), were highly dynamic, exhibiting mainly transient associations with chromatin, contrary to Pol II, which formed more stable chromatin interactions...
July 19, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28723583/label-free-high-resolution-3-d-imaging-of-gold-nanoparticles-inside-live-cells-using-optical-diffraction-tomography
#7
Doyeon Kim, Nuri Oh, Kyoohyun Kim, SangYun Lee, Chan-Gi Pack, Ji-Ho Park, YongKeun Park
Delivery of gold nanoparticles (GNPs) into live cells has high potentials, ranging from molecular-specific imaging, photodiagnostics, to photothermal therapy. However, studying the long-term dynamics of cells with GNPs using conventional fluorescence techniques suffers from phototoxicity and photobleaching. Here, we present a method for 3-D imaging of GNPs inside live cells exploiting refractive index (RI) as imaging contrast. Employing optical diffraction tomography, 3-D RI tomograms of live cells with GNPs are precisely measured for an extended period with sub-micrometer resolution...
July 16, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28723114/simultaneous-imaging-of-three-tumor-related-mrnas-in-living-cells-with-a-dna-tetrahedron-based-multicolor-nanoprobe
#8
Song Wang, Mengchan Xia, Jie Liu, Sichun Zhang, Xinrong Zhang
We constructed a DNA tetrahedron based multicolor nanoprobe, which could simultaneously imaging of three tumor-related mRNAs in living cells through fluorescence restoration caused by competitive chain replacement reaction. The oligonucleotides used to construct the tetrahedron were extended by adding three 21-base recognition sequences modified with different fluorophores (FAM, Cy3, and Cy5) in the 5' end. Three 11-base complementary sequences modified with quencher (BHQ1 for FAM and BHQ2 for Cy3 and Cy5) were hybridized with the recognition sequences to quench the fluorescence...
June 23, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28722423/generation-of-a-cgmp-indicator-with-an-expanded-dynamic-range-by-optimization-of-amino-acid-linkers-between-a-fluorescent-protein-and-pde5%C3%AE
#9
Shogo Matsuda, Kazuki Harada, Motoki Ito, Mai Takizawa, Devina Wongso, Takashi Tsuboi, Tetsuya Kitaguchi
Here we describe the development of a single fluorescent protein (FP)-based cGMP indicator, Green cGull, based on the cGMP binding domain from mouse phosphodiesterase 5α. The dynamic range of Green cGull was enhanced to a 7.5-fold fluorescence change upon cGMP binding by optimization of the amino acid linkers between the cGMP binding domain and FP. Green cGull has excitation and emission peaks at 498 and 522 nm, respectively, and specifically responds to cGMP in a dose-dependent manner. Live cell imaging analysis revealed that addition of a nitric oxide (NO) donor induced different cGMP kinetics and was cell-type dependent...
January 27, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28720840/positioning-of-the-scrambled-receptor-requires-udp-glc-sterol-glucosyltransferase-80b1-in-arabidopsis-roots
#10
Victoria G Pook, Meera Nair, KookHui Ryu, James C Arpin, John Schiefelbein, Kathrin Schrick, Seth DeBolt
The biological function of sterol glucosides (SGs), the most abundant sterol derivatives in higher plants, remains uncertain. In an effort to improve our understanding of these membrane lipids we examined phenotypes exhibited by the roots of Arabidopsis (Arabidopsis thaliana) lines carrying insertions in the UDP-Glc:sterol glucosyltransferase genes, UGT80A2 and UGT80B1. We show that although ugt80A2 mutants exhibit significantly lower levels of total SGs they are morphologically indistinguishable from wild-type plants...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28720785/evidence-of-multi-domain-morphological-structures-in-living-escherichia-coli
#11
Sharareh Tavaddod, Hossein Naderi-Manesh
A combination of light-microscopy and image processing was used to elaborate on the fluctuation in the width of the cylindrical part of Escherichia coli at sub-pixel-resolution, and under in vivo conditions. The mean-squared-width-difference along the axial direction of the cylindrical part of a number of bacteria was measured. The results reveal that the cylindrical part of Escherichia coli is composed of multi-domain morphological structures. The length of the domains starts at 150 nm in newborn cells, and linearly increases in length up to 300 nm in aged cells...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28718799/zebrafish-as-a-model-organism-for-the-development-of-drugs-for-skin-cancer
#12
REVIEW
Fatemeh Bootorabi, Hamed Manouchehri, Reza Changizi, Harlan Barker, Elisabetta Palazzo, Annalisa Saltari, Mataleena Parikka, Carlo Pincelli, Ashok Aspatwar
Skin cancer, which includes melanoma and squamous cell carcinoma, represents the most common type of cutaneous malignancy worldwide, and its incidence is expected to rise in the near future. This condition derives from acquired genetic dysregulation of signaling pathways involved in the proliferation and apoptosis of skin cells. The development of animal models has allowed a better understanding of these pathomechanisms, with the possibility of carrying out toxicological screening and drug development. In particular, the zebrafish (Danio rerio) has been established as one of the most important model organisms for cancer research...
July 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28718621/illuminating-the-cell-s-biochemical-activity-architecture
#13
Sohum Mehta, Jin Zhang
All cellular behaviors arise through the coordinated actions of numerous intracellular biochemical pathways. Over the past twenty years, efforts to probe intracellular biochemical processes have undergone a fundamental transformation brought about by advances in fluorescence imaging, such as the development of genetically encoded fluorescent reporters and new imaging technologies; the impact of these approaches on our understanding of the molecular underpinnings of biological function cannot be understated...
July 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28718466/exploring-viscosity-polarity-and-temperature-sensitivity-of-bodipy-based-molecular-rotors
#14
Aurimas Vyšniauskas, Ismael López-Duarte, Nicolas Duchemin, Thanh-Truc Vu, Yilei Wu, Ekaterina M Budynina, Yulia A Volkova, Eduardo Peña Cabrera, Diana E Ramírez-Ornelas, Marina K Kuimova
Microviscosity is a key parameter controlling the rate of diffusion and reactions on the microscale. One of the most convenient tools for measuring microviscosity is by fluorescent viscosity sensors termed 'molecular rotors'. BODIPY-based molecular rotors in particular proved extremely useful in combination with fluorescence lifetime imaging microscopy, for providing quantitative viscosity maps of living cells as well as measuring dynamic changes in viscosity over time. In this work, we investigate several new BODIPY-based molecular rotors with the aim of improving on the current viscosity sensing capabilities and understanding how the structure of the fluorophore is related to its function...
July 18, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28717898/thiophene-based-rhodamine-as-selectivef-luorescence-probe-for-fe-iii-and-al-iii-in-living-cells
#15
Kun-Peng Wang, Ju-Peng Chen, Si-Jie Zhang, Yang Lei, Hua Zhong, Shaojin Chen, Xin-Hong Zhou, Zhi-Qiang Hu
The thiophene-modified rhodamine 6G (GYJ) has been synthesized as a novel chemosensor. The sensor has sufficiently high selectivity and sensitivity for the detection of Fe(3+) and Al(3+) ions (M(3+)) by fluorescence and ultraviolet spectroscopy with a strong ability for anti-interference performance. The binding ratio of M(3+)-GYJ complex was determined to be 2:1 according to the Job's plot. The binding constants for Fe(3+) and Al(3+) were calculated to be 3.91 × 10(8) and 5.26 × 10(8) M(-2), respectively...
July 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28717571/visible-spectrum-extended-focus-optical-coherence-microscopy-for-label-free-sub-cellular-tomography
#16
Paul J Marchand, Arno Bouwens, Daniel Szlag, David Nguyen, Adrien Descloux, Miguel Sison, Séverine Coquoz, Jérôme Extermann, Theo Lasser
We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from a supercontinuum laser source. As the spectrum spans from near-infrared to visible wavelengths (240 nm in bandwidth), we call the system visOCM. The combination of such a broad spectrum with a high-NA objective creates an almost isotropic 3D submicron resolution...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28716648/applications-of-high-speed-atomic-force-microscopy-to-real-time-visualization-of-dynamic-biomolecular-processes
#17
REVIEW
Takayuki Uchihashi, Simon Scheuring
BACKGROUND: Many biological processes in a living cell are consequences of sequential and hierarchical dynamic events of biological macromolecules such as molecular interactions and conformational changes. Hence, knowledge of structures, assembly and dynamics of proteins is the foundation for understanding how biological molecules work. Among several techniques to analyze dynamics of proteins, high-speed atomic force microscopy (HS-AFM) is unique to provide direct information about both structure and dynamics of single proteins at work...
July 14, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28715768/dynamic-complexity-plant-receptor-complexes-at-the-plasma-membrane
#18
REVIEW
Rebecca C Burkart, Yvonne Stahl
Plant receptor complexes at the cell surface perceive many different external and internal signalling molecules and relay these signals into the cell to regulate development, growth and immunity. Recent progress in the analyses of receptor complexes using different live cell imaging approaches have shown that receptor complex formation and composition are dynamic and take place at specific microdomains at the plasma membrane. In this review we focus on three prominent examples of Arabidopsis thaliana receptor complexes and how their dynamic spatio-temporal distribution at the PM has been studied recently...
July 13, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28715691/a-quinoline-based-fluorometric-and-colorimetric-dual-modal-ph-probe-and-its-application-in-bioimaging
#19
Qin Zhu, Zhao Li, Lan Mu, Xi Zeng, Carl Redshaw, Gang Wei
The compound (E)-8-hydroxyl-2-[(E)-2-(2, 4-dihydroxyphenyl)vinyl]-quinoline (1) has been developed as a fluorometric and colorimetric dual-modal probe for pH detection in solution and in vivo. Remarkable changes in the fluorescence intensity with large Stokes shifts and colorimetric responses were observed as a function of pH. The sensing mechanisms involving protonation and deprotonation processes over the acidic and alkaline pH ranges were confirmed by (1)H NMR and IR spectroscopic analysis. Furthermore, the application of probe 1 for the imaging of live PC3 cells was successfully achieved...
July 2, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28715402/fluorescent-dye-labeling-of-erythrocytes-and-leukocytes-for-studying-the-flow-dynamics-in-mouse-retinal-circulation
#20
Rupesh Agrawal, Praveen Kumar Balne, Sai Bo Bo Tun, Yeo Sia Wey, Neha Khandelwal, Veluchamy A Barathi
The retinal and choroidal blood flow dynamics may provide insight into the pathophysiology and sequelae of various ocular diseases, such as glaucoma, diabetic retinopathy, age-related macular degeneration (AMD) and other ocular inflammatory conditions. It may also help to monitor the therapeutic responses in the eye. The proper labeling of the blood cells, coupled with live-cell imaging of the labeled cells, allows for the investigation of the flow dynamics in the retinal and choroidal circulation. Here, we describe the standardized protocols of 1...
July 3, 2017: Journal of Visualized Experiments: JoVE
keyword
keyword
23355
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"