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https://www.readbyqxmd.com/read/28227386/image-classification-of-unlabeled-malaria-parasites-in-red-blood-cells
#1
Zheng Zhang, L L Sharon Ong, Kong Fang, Athul Matthew, Justin Dauwels, Ming Dao, Harry Asada, Zheng Zhang, L L Sharon Ong, Kong Fang, Athul Matthew, Justin Dauwels, Ming Dao, Harry Asada, Justin Dauwels, Zheng Zhang, Kong Fang, Ming Dao, Harry Asada
This paper presents a method to detect unlabeled malaria parasites in red blood cells. The current "gold standard" for malaria diagnosis is microscopic examination of thick blood smear, a time consuming process requiring extensive training. Our goal is to develop an automate process to identify malaria infected red blood cells. Major issues in automated analysis of microscopy images of unstained blood smears include overlapping cells and oddly shaped cells. Our approach creates robust templates to detect infected and uninfected red cells...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226849/investigation-of-the-ca-2-response-of-human-hnt-astrocytes-to-laser-removal-of-cellular-processes
#2
Brad J Raos, E Scott Graham, Charles P Unsworth, Brad J Raos, E Scott Graham, Charles P Unsworth, Charles P Unsworth, Brad J Raos, E Scott Graham
We demonstrate, for the first time, UV laser ablative microsurgery as a method for pruning astrocytic processes from live hNT astrocytic networks in vitro. Calcium fluorescence imaging was used to evaluate the cellular response to process ablation. The results showed that ablation of astrocyte processes induced an immediate increase in intracellular calcium level which propagated through the cells cytoplasm as a wave originating from the ablation site. The increased intracellular calcium dissipated from the body of the cell but remained high in the vicinity of the ablation site...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28221782/the-cation-%C3%AF-interaction-enables-a-halo-tag-fluorogenic-probe-for-fast-no-wash-live-cell-imaging-and-gel-free-protein-quantification
#3
Yu Liu, Kun Miao, Noah P Dunham, Hongbin Liu, Matthew Fares, Amie K Boal, Xiaosong Li, Xin Zhang
Design of fluorogenic probes for Halo-tag is highly desirable but challenging. Previous work achieved this goal by controlling the chemical switch of spirolactones upon the covalent conju-gation between Halo-tag and probes or by incorporating a 'channel dye' into the substrate bind-ing tunnel of Halo-tag. In this work, we have developed a novel class of Halo-tag fluorogenic probes that are derived from solvatochromic fluorophores. The optimal probe, harboring a ben-zothiadiazole scaffold, exhibits a 1000-fold fluorescence enhancement upon reaction with Halo-tag...
February 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28221751/molecular-engineering-and-design-of-semiconducting-polymer-dots-with-narrow-band-near-infrared-emission-for-in-vivo-biological-imaging
#4
Chi-Shiang Ke, Chia-Chia Fang, Jia-Ying Yan, Po-Jung Tseng, Joseph R Pyle, Chuan-Pin Chen, Shu-Yi Lin, Jixin Chen, Xuanjun Zhang, Yang-Hsiang Chan
This article describes the design and synthesis of donor-bridge-acceptor based semiconducting polymer dots (Pdots) that exhibit narrow-band emissions, ultrahigh brightness, and large Stokes shifts in the near-infrared (NIR) region. We systematically investigated the effect of π-bridges on the fluorescence quantum yields of the donor-bridge-acceptor based Pdots. The Pdots could be excited by a 488-nm or 532-nm laser and have a high fluorescence quantum yield of 33% with a Stokes shift over 200 nm. The emission full width at half-maximum of the Pdots can be as narrow as 29 nm, about 2...
February 21, 2017: ACS Nano
https://www.readbyqxmd.com/read/28221392/a-4-5-quinolimide-based-fluorescent-sensor-for-the-turn-on-detection-of-cd-2-with-live-cell-imaging
#5
Yu Zhang, Xiangfeng Guo, Mengmeng Zheng, Rui Yang, Hongming Yang, Lihua Jia, Mengmeng Yang
A 4,5-quinolimide derivative, BNA, bearing the amide-DPA receptor, was synthesized as a turn-on fluorescent sensor for Cd(2+). Under physiological conditions, BNA could distinguish Cd(2+) from Zn(2+), showing turn-on fluorescence behaviour and an increased fluorescence lifetime. BNA and Cd(2+) formed a 1 : 1 stoichiometric complex, and the detection limit was measured to be as low as 11 nM. Furthermore, BNA was utilized for fluorescence imaging of Cd(2+) in live cells. To the best of our knowledge, it is the first 4,5-quinolimide-based sensor for the detection of metal ions...
February 21, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28221345/rational-design-of-reversible-fluorescent-probes-for-live-cell-imaging-and-quantification-of-fast-glutathione-dynamics
#6
Keitaro Umezawa, Masafumi Yoshida, Mako Kamiya, Tatsuya Yamasoba, Yasuteru Urano
Alterations in glutathione (GSH) homeostasis are associated with a variety of diseases and cellular functions, and therefore, real-time live-cell imaging and quantification of GSH dynamics are important for understanding pathophysiological processes. However, existing fluorescent probes are unsuitable for these purposes due to their irreversible fluorogenic mechanisms or slow reaction rates. In this work, we have successfully overcome these problems by establishing a design strategy inspired by Mayr's work on nucleophilic reaction kinetics...
March 2017: Nature Chemistry
https://www.readbyqxmd.com/read/28220278/rhodamine-diaminomaleonitrile-conjugate-as-a-novel-colorimetric-fluorescent-sensor-for-recognition-of-cd-2-ion
#7
Perumal Sakthivel, Karuppannan Sekar, Gandhi Sivaraman, Subramanian Singaravadivel
Rhodamine diaminomaleonitrile linked probe (RD-1) shows highly sensitive colorimetric and selective turn-on fluorescent response to Cd(2+) over other metal ions. The fluorescence intensity and absorbance of the probe RD-1 showed a good linearity, with very low detection limits of 18.5 nm. The probe RD-1 was preliminarily applied to the determination of Cd(2+) ion in water samples from river and tap water with satisfying results. The live cell image confocal microscopy, HeLa cell demonstrated that RD-1 had low cytotoxicity with good membrane permeable property is successfully applied to fluorescence microscopic imaging for the detection of Cd(2+) ions...
February 21, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28219700/inhibition-of-ros-production-autophagy-or-apoptosis-signaling-reversed-the-anticancer-properties-of-antrodia-salmonea-in-triple-negative-breast-cancer-mda-mb-231-cells
#8
Chia-Ting Chang, Mallikarjuna Korivi, Hui-Chi Huang, Varadharajan Thiyagarajan, Kai-Yuan Lin, Pei-Jane Huang, Jer-Yuh Liu, You-Cheng Hseu, Hsin-Ling Yang
We investigated the in vitro and in vivo anticancer properties of Antrodia salmonea (AS), a well-known edible/medicinal mushroom in Taiwan, on human triple-negative breast cancer (MDA-MB-231) cells and xenografted nude mice; and revealed the underlying molecular mechanisms involved in autophagic- and apoptotic-cell death. Treatment of MDA-MB-231 cells with fermented culture broth of AS (0-200 μg/mL) inhibited cell viability/growth. AS-induced autophagy was evidenced via increased LC3-II accumulation, GFP-LC3 puncta and AVOs formation in MDA-MB-231 cells...
February 17, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28218744/live-cell-imaging-to-compare-the-transfection-and-gene-silencing-efficiency-of-calcium-phosphate-nanoparticles-and-a-liposomal-transfection-agent
#9
S Chernousova, M Epple
The processing of DNA (for transfection) and siRNA (for gene silencing), introduced into HeLa cells by triple-shell calcium phosphate nanoparticles, was followed by live-cell imaging. For comparison, the commercial liposomal transfection agent Lipofectamine(TM) was used. The cells were incubated with these delivery systems, carrying either eGFP-encoding DNA or siRNA against eGFP. In the latter case, HeLa cells which stably expressed eGFP were used. The expression of eGFP started after 5 h in the case of nanoparticles and after 4 h in the case of Lipofectamine...
February 20, 2017: Gene Therapy
https://www.readbyqxmd.com/read/28218723/a-biocompatible-colorimetric-triphenylamine-dicyanovinyl-conjugated-fluorescent-probe-for-selective-and-sensitive-detection-of-cyanide-ion-in-aqueous-media-and-living-cells
#10
Zi-Hua Zheng, Zhi-Ke Li, Lin-Jiang Song, Qi-Wei Wang, Qing-Fei Huang, Li Yang
A colorimetric and turn-on fluorescent probe 1 bearing triphenylamine-thiophene and dicyanovinyl groups has been synthesized and used to detect cyanide anion via a nucleophilic addition reaction. Probe 1 exhibited prominent selectivity and sensitivity towards CN(-) in aqueous media, even in the presence of other anions such as S(2-), HS(-), SO₃(2-), S₂O₃(2-), S₂O₈(2-), I(-), Br(-), Cl(-), F(-), NO₂(-), N₃(-), SO₄(2-), SCN(-), HCO₃(-), CO₃(2-) and AcO(-). Moreover, a low detection limit (LOD, 51 nM) was observed...
February 19, 2017: Sensors
https://www.readbyqxmd.com/read/28218511/engineering-of-radioiodine-labeled-gold-core-shell-nanoparticles-as-efficient-nuclear-medicine-imaging-agents-for-trafficking-of-dendritic-cells
#11
Sang Bong Lee, Sang-Woo Lee, Shin Young Jeong, GhilSuk Yoon, Sung Jin Cho, Sang Kyoon Kim, In-Kyu Lee, Byeong-Cheol Ahn, Jaetae Lee, Yong Hyun Jeon
The development of highly sensitive, stable, and biocompatible imaging agents allowing visualization of dendritic cell (DC) migration is one of the essential factors for effective DC-based immunotherapy. Here, we used a novel and efficient synthesis approach to develop Radioiodine-124-labeled Tannic Acid Gold Core-Shell Nanoparticles (124I-TA-Au@AuNPs) for DC labeling and in vivo tracking of their migration using positron emission tomography (PET). 124I-TA-Au@AuNPs were produced within 40 min in high yield via straightforward tannic acid-mediated radiolabeling chemistry and incorporation of Au shell, which resulted in high radio-sensitivity and excellent chemical stability of nanoparticles in DCs and living mice...
February 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28218503/long-term-tissue-culture-of-adult-brain-and-spleen-slices-on-nanostructured-scaffolds
#12
Sonja Kallendrusch, Felicitas Merz, Ingo Bechmann, Stefan G Mayr, Mareike Zink
Long-term tissue culture of adult mammalian organs is a highly promising approach to bridge the gap between single cell cultures and animal experiments, and bears the potential to reduce in vivo studies. Novel biomimetic materials open up new possibilities to maintain the complex tissue structure in vitro; however, survival times of adult tissues ex vivo are still limited to a few days with established state-of-the-art techniques. Here, it is demonstrated that TiO2 nanotube scaffolds with specific tissue-tailored characteristics can serve as superior substrates for long-term adult brain and spleen tissue culture...
February 20, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28216026/cyclic-nucleotide-imaging-and-cardiovascular-disease
#13
REVIEW
Filip Berisha, Viacheslav O Nikolaev
The universal second messengers cyclic nucleotides 3',5'-cyclic adenosine monophosphate (cAMP) and 3',5'-cyclic guanosine monophosphate (cGMP) play central roles in cardiovascular function and disease. They act in discrete, functionally relevant subcellular microdomains which regulate, for example, calcium cycling and excitation-contraction coupling. Such localized cAMP and cGMP signals have been difficult to measure using conventional biochemical techniques. Recent years have witnessed the advent of live cell imaging techniques which allow visualization of these functionally relevant second messengers with unprecedented spatial and temporal resolution at cellular, subcellular and tissue levels...
February 16, 2017: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28215939/live-imaging-analysis-of-germ-cell-proliferation-in-the-c-elegans-adult-supports-a-stochastic-model-for-stem-cell-proliferation
#14
Simona Rosu, Orna Cohen-Fix
The C. elegans adult hermaphrodite contains a renewable pool of mitotically dividing germ cells that are contained within the progenitor zone (PZ), at the distal region of the germline. From the PZ, cells enter meiosis and differentiate, ensuring the continued production of oocytes. In this study, we investigated the proliferation strategy used to maintain the PZ pool by using a photoconvertible marker to follow the fate of selected germ cells and their descendants in live worms. We found that the most distal pool of 6-8 rows of cells in the PZ (the distal third) behave similarly, with a fold expansion corresponding to one cell division every 6hours on average...
February 16, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28215338/investigating-symmetry-breaking-in-yeast-from-seeing-to-understanding
#15
P Singh, A Das, R Li
Diverse cellular functions require the establishment of cell polarity. Although the morphological manifestation of cell polarity can be complex, common principles and conserved regulatory pathways can be studied leveraging the powerful genetic tools in the budding yeast Saccharomyces cerevisiae. A vast array of genetic and cell biological tools and interdisciplinary approaches have been employed to understand the establishment and maintenance of cell polarity in budding yeast. These tools enabled the identification of key molecular components, their mechanisms of action, and the pathway governing the spatiotemporal properties of cell polarity...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28215337/in-vivo-analysis-of-morphogenesis-in-plants
#16
T Stanislas, O Hamant, J Traas
Although many molecular regulators of morphogenesis have been identified in plants, it remains largely unknown how the molecular networks influence local cell shape and how cell growth, form, and position are coordinated during tissue and organ formations. So far, analyses of gene function in morphogenesis have mainly focused on the qualitative analysis of phenotypes, often providing limited mechanistic insight into how particular factors act. For this reason, there has been a growing interest in mathematical and computational models to formalize and test hypotheses...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28215332/fluorescence-exclusion-measurement-of-volume-in-live-cells
#17
C Cadart, E Zlotek-Zlotkiewicz, L Venkova, O Thouvenin, V Racine, M Le Berre, S Monnier, M Piel
Volume is a basic physical property of cells; however, it has been poorly investigated in cell biology so far, mostly because it is difficult to measure it precisely. Recently, large efforts were made to experimentally measure mammalian cell size and used mass, density, or volume as proxies for cell size. Here, we describe a method enabling cell volume measurements for single living cells. The method is based on the principle of fluorescent dye exclusion and can be easily implemented in cell biology laboratories...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28214774/multimodality-imaging-of-bone-marrow-derived-dendritic-cell-migration-and-antitumor-immunity
#18
Su-Bi Ahn, Sang Bong Lee, Thoudam Debraj Singh, Sung Jin Cho, Sang Kyoon Kim, In-Kyu Lee, Shin Young Jeong, Byeong-Cheol Ahn, Jaetae Lee, Sang-Woo Lee, Yong Hyun Jeon
Here, we sought to monitor bone marrow-derived dendritic cell (BMDC) migration and antitumor effects using a multimodal reporter imaging strategy in living mice. BMDCs were transduced with retroviral vector harboring human sodium iodide symporter (hNIS, nuclear imaging reporter), firefly luc2 (optical imaging reporter), and thy1.1 (surrogate marker of NIS and luc2) genes (BMDC/NF cells). No significant differences in biological functions, including cell proliferation, antigen uptake, phenotype expression, and migration ability, were observed between BMDC and BMDC/NF cells...
February 16, 2017: Translational Oncology
https://www.readbyqxmd.com/read/28214023/using-aequorin-probes-to-measure-ca-2-in-intracellular-organelles
#19
REVIEW
María Teresa Alonso, Macarena Rodríguez-Prados, Paloma Navas-Navarro, Jonathan Rojo-Ruiz, Javier García-Sancho
Aequorins are excellent tools for measuring intra-organellar Ca(2+) and assessing its role in physiological and pathological functions. Here we review targeting strategies to express aequorins in various organelles. We address critical topics such as probe affinity tuning as well as normalization and calibration of the signal. We also focus on bioluminescent Ca(2+) imaging in nucleus or mitochondria of living cells. Finally, recent advances with a new chimeric GFP-aequorin protein (GAP), which can be used either as luminescent or fluorescent Ca(2+) probe, are presented...
January 16, 2017: Cell Calcium
https://www.readbyqxmd.com/read/28212708/development-and-performance-evaluation-of-a-three-dimensional-clinostat-synchronized-heavy-ion-irradiation-system
#20
Hiroko Ikeda, Hikaru Souda, Anggraeini Puspitasari, Kathryn D Held, Jun Hidema, Takeshi Nikawa, Yukari Yoshida, Tatsuaki Kanai, Akihisa Takahashi
Outer space is an environment characterized by microgravity and space radiation, including high-energy charged particles. Astronauts are constantly exposed to both microgravity and radiation during long-term stays in space. However, many aspects of the biological effects of combined microgravity and space radiation remain unclear. We developed a new three-dimensional (3D) clinostat synchronized heavy-ion irradiation system for use in ground-based studies of the combined exposures. Our new system uses a particle accelerator and a respiratory gating system from heavy-ion radiotherapy to irradiate samples being rotated in the 3D clinostat with carbon-ion beams only when the samples are in the horizontal position...
February 2017: Life Sciences in Space Research
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