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https://www.readbyqxmd.com/read/28441582/synthesis-and-antiproteasomal-activity-of-novel-o-benzyl-salicylamide-based-inhibitors-built-from-leucine-and-phenylalanine
#1
Radek Jorda, Jan Dušek, Eva Řezníčková, Karel Pauk, Pratibha P Magar, Aleš Imramovský, Vladimír Kryštof
Inhibition of protein degradation is one of strategies for suppression of uncontrolled proliferation of cancer cells. Proteolytic degradation in cells is mainly ensured by proteasome and its inhibition by bortezomib showed benefit in clinical use for the treatment of multiple myeloma. We report here the library of antiproteasomal O-benzyl salicylamides built from leucine and phenylalanine. Prepared compounds displayed antiproliferative activity on K562, CEM and U266 cancer cell lines, ranging from high micromolar to submicromolar GI50 values...
April 15, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28440546/stem-cell-dynamics-in-muscle-regeneration-insights-from-live-imaging-in-different-animal-models
#2
REVIEW
Dhanushika Ratnayake, Peter D Currie
In recent years, live imaging has been adopted to study stem cells in their native environment at cellular resolution. In the skeletal muscle field, this has led to visualising the initial events of muscle repair in mouse, and the entire regenerative response in zebrafish. Here, we review recent discoveries in this field obtained from live imaging studies. Tracking of tissue resident stem cells, the satellite cells, following injury has captured the morphogenetic dynamics of stem/progenitor cells as they facilitate repair...
April 25, 2017: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
https://www.readbyqxmd.com/read/28440317/live-cell-imaging-of-mitochondria-following-targeted-irradiation-in-situ-reveals-rapid-and-highly-localized-loss-of-membrane-potential
#3
Dietrich W M Walsh, Christian Siebenwirth, Christoph Greubel, Katarina Ilicic, Judith Reindl, Stefanie Girst, Giovanna Muggiolu, Marina Simon, Philippe Barberet, Hervé Seznec, Hans Zischka, Gabriele Multhoff, Thomas E Schmid, Guenther Dollinger
The reliance of all cell types on the mitochondrial function for survival makes mitochondria an interesting target when trying to understand their role in the cellular response to ionizing radiation. By harnessing highly focused carbon ions and protons using microbeams, we have performed in situ live cell imaging of the targeted irradiation of individual mitochondria stained with Tetramethyl rhodamine ethyl ester (TMRE), a cationic fluorophore which accumulates electrophoretically in polarized mitochondria...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28439953/feasibility-trial-for-primary-stroke-prevention-in-children-with-sickle-cell-anemia-in-nigeria-spin-trial
#4
Najibah Galadanci, Shehu Umar Abdullahi, Leah D Vance, Abdulkadir Musa Tabari, Shehi Ali, Raymond Belonwu, Auwal Salihu, Aisha Amal Galadanci, Binta Wudil Jibir, Halima Bello-Manga, Kathleen Neville, Fenella J Kirkham, Yu Shyr, Sharon Phillips, Brittany V Covert, Adetola A Kassim, Lori C Jordan, Muktar H Aliyu, Michael R DeBaun
The vast majority of children with sickle cell anemia (SCA) live in Africa, where evidence-based guidelines for primary stroke prevention are lacking. In Kano, Nigeria, we conducted a feasibility trial to determine the acceptability of hydroxyurea therapy for primary stroke prevention in children with abnormal transcranial Doppler (TCD) measurements. Children with SCA and abnormal non-imaging TCD measurements (≥ 200 cm/s) received moderate fixed-dose hydroxyurea therapy (∼20 mg/kg/day). A comparison group of children with TCD measurements < 200 cm/s was followed prospectively...
April 25, 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/28439864/long-term-confocal-imaging-of-arabidopsis-thaliana-roots-for-simultaneous-quantification-of-root-growth-and-fluorescent-signals
#5
Delyana Stoeva, Christian Göschl, Bruce Corliss, Wolfgang Busch
Observing cellular and molecular processes in living organisms is key for understanding many important biological processes. Confocal microscopy is excellently suited for this as it enables the observation of molecules and cells in tissue layers of living organisms in three dimensions over time. However, in continuously growing organs, such as plant roots, observations over extended time spans become difficult as the specimen quickly grows out the field of view. Here, we provide a protocol that allows for the acquisition of confocal microscope time-lapse images of root tips spanning many hours, as the growing root tip is tracked and the microscopy is automatized to change the position of the stage...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28439090/slc10a4-regulates-ige-mediated-mast-cell-degranulation-in-vitro-and-mast-cell-mediated-reactions-in-vivo
#6
Hanna Pettersson, Behdad Zarnegar, Annika Westin, Viktor Persson, Christiane Peuckert, Jörgen Jonsson, Jenny Hallgren, Klas Kullander
Mast cells act as sensors in innate immunity and as effector cells in adaptive immune reactions. Here we demonstrate that SLC10A4, also referred to as the vesicular aminergic-associated transporter, VAAT, modifies mast cell degranulation. Strikingly, Slc10a4 (-/-) bone marrow-derived mast cells (BMMCs) had a significant reduction in the release of granule-associated mediators in response to IgE/antigen-mediated activation, whereas the in vitro development of mast cells, the storage of the granule-associated enzyme mouse mast cell protease 6 (mMCP-6), and the release of prostaglandin D2 and IL-6 were normal...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28438704/folic-acid-decorated-polyamidoamine-dendrimer-exhibits-high-tumor-uptake-and-sustained-highly-localized-retention-in-solid-tumors-its-utility-for-local-sirna-delivery
#7
Leyuan Xu, W Andrew Yeudall, Hu Yang
The utility of folic acid (FA)-decorated polyamidoamine dendrimer G4 (G4-FA) as a vector was investigated for local delivery of siRNA. In a xenograft HN12 (or HN12-YFP) tumor mouse model of head and neck squamous cell carcinomas (HNSCC), intratumorally (i.t.) injected G4-FA exhibited high tumor uptake and sustained highly localized retention in the tumors according to near infrared (NIR) imaging assessment. siRNA against vascular endothelial growth factor A (siVEGFA) was chosen as a therapeutic modality. Compared to the nontherapeutic treatment groups (PBS solution or dendrimer complexed with nontherapeutic green fluorescent protein siRNA [siGFP]), G4-FA/siVEGFA showed tumor inhibition effects in single-dose and two-dose regimen studies...
April 21, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28438675/integrating-cell-biology-and-proteomic-approaches-in-plants
#8
Tomáš Takáč, Olga Šamajová, Jozef Šamaj
Significant improvements of protein extraction, separation, mass spectrometry and bioinformatics nurtured advancements of proteomics during the past years. The usefulness of proteomics in the investigation of biological problems can be enhanced by integration with other experimental methods from cell biology, genetics, biochemistry, pharmacology, molecular biology and other omics approaches including transcriptomics and metabolomics. This review aims to summarize current trends integrating cell biology and proteomics in plant science...
April 21, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28438609/novel-replicons-and-trans-encapsidation-systems-for-hepatitis-c-virus-proteins-live-imaging-and-virus-host-interaction-proteomics
#9
Ovidiu Vlaicu, Tudor Selescu, Florin Pastrama, Cristian Munteanu, Laura Riva, Jean Dubuisson, Yves Rouille, Costin-Ioan Popescu
Proteomics and imaging techniques are used more and more in tandem to investigate the virus-host interaction. Herein we present novel replicons, methods and trans-encapsidation systems suitable for determination of Hepatitis C Virus (HCV) proteins interactomes and live imaging of viral proteins dynamics in HCV cell culture (HCVcc) system. To identify endogenous factors involved in the HCV life cycle, we constructed full-length functional replicons with affinity purification (AP) tags fused to NS2 and NS5A proteins...
April 21, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28436652/observation-of-the-generation-of-onoo-in-mitochondria-under-various-stimuli-with-a-sensitive-fluorescence-probe
#10
Hongyu Li, Xiaohua Li, Xiaofeng Wu, Wen Shi, Huimin Ma
Peroxynitrite (ONOO-), an important reactive oxygen species (ROS), may be generated in the mitochondria under various physiological and pathological conditions. In this work, we designed and synthesized a mitochondria-targetable fluorescence probe RPTPP by using rhodamine as the fluorophore, phenylhydrazine as the recognition moiety, and triphenyl- phosphonium cation as the mitochondria-targeting moiety. Upon reaction of the probe with ONOO-, the oxidation of phenylhydrazine by ONOO- and the subsequent hydrolysis opens the non-fluorescent spirocyclic structure and thus triggers a fluorescence turn-on response, which provides a sensitive and selective method for the detection of ONOO-...
April 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28436528/a-highly-selective-and-sensitive-fluorescent-probe-for-cu-2-based-on-a-novel-naphthalimide-rhodamine-platform-and-its-application-in-live-cell-imaging
#11
Chang Liu, Xiaojie Jiao, Song He, Liancheng Zhao, Xianshun Zeng
Copper plays important roles in a variety of fundamental physiological processes. At the cell organelle level, aberrant copper homeostasis in lysosomes can lead to various serious diseases. Herein, a bifluorophore-based, lysosome-targetable Cu(2+)-selective ratiometric fluorescent probe (V) has been synthesized by reasonable design. The probe V shows high selectivity toward Cu(2+) ions over other cations and exhibits high sensitivity (1.45 nM) for the detection of Cu(2+) ions. Meanwhile, the probe is cell permeable and suitable for ratiometric visualization of lysosomal Cu(2+) in the living cell...
April 24, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28436473/volumetric-chemical-imaging-by-stimulated-raman-projection-microscopy-and-tomography
#12
Xueli Chen, Chi Zhang, Peng Lin, Kai-Chih Huang, Jimin Liang, Jie Tian, Ji-Xin Cheng
Volumetric imaging allows global understanding of three-dimensional (3D) complex systems. Light-sheet fluorescence microscopy and optical projection tomography have been reported to image 3D volumes with high resolutions and at high speeds. Such methods, however, usually rely on fluorescent labels for chemical targeting, which could perturb the biological functionality in living systems. We demonstrate Bessel-beam-based stimulated Raman projection (SRP) microscopy and tomography for label-free volumetric chemical imaging...
April 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28435770/thermally-activated-delayed-fluorescence-organic-dots-tadf-odots-for-time-resolved-and-confocal-fluorescence-imaging-in-living-cells-and-in-vivo
#13
Tingting Li, Dongliang Yang, Liuqing Zhai, Suiliang Wang, Baomin Zhao, Nina Fu, Lianhui Wang, Youtian Tao, Wei Huang
The fluorophores with long-lived fluorescent emission are highly desirable for time-resolved fluorescence imaging (TRFI) in monitoring target fluorescence. By embedding the aggregates of a thermally activated delayed fluorescence (TADF) dye, 2,3,5,6-tetracarbazole-4-cyano-pyridine (CPy), in distearoyl-sn-glycero-3-phosphoethanolamine-poly(ethylene glycol) (DSPE-PEG2000) matrix, CPy-based organic dots (CPy-Odots) with a long fluorescence lifetime of 9.3 μs (in water at ambient condition) and high brightness (with an absolute fluorescence quantum efficiency of 38...
April 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28435463/design-and-development-of-fluorescent-vemurafenib-analogs-for-in-vivo-imaging
#14
Hannes Mikula, Shawn Stapleton, Rainer H Kohler, Claudio Vinegoni, Ralph Weissleder
Herein we describe fluorescent derivatives of vemurafenib to probe therapeutic BRAF inhibition in live cells and in vivo. The compounds were evaluated and compared by determining target binding, inhibition of mutant BRAF melanoma cell lines and live cell imaging. We show that vemurafenib-BODIPY is a superior imaging drug to visualize the targets of vemurafenib in live cells and in vivo in non-resistant and resistant melanoma tumors.
2017: Theranostics
https://www.readbyqxmd.com/read/28434902/intravital-microscopy-of-biosensor-activities-and-intrinsic-metabolic-states
#15
REVIEW
Seth Winfree, Takashi Hato, Richard N Day
Intravital microscopy (IVM) is an imaging tool that is capable of detecting subcellular signaling or metabolic events as they occur in tissues in the living animal. Imaging in highly scattering biological tissues, however, is challenging because of the attenuation of signal in images acquired at increasing depths. Depth-dependent signal attenuation is the major impediment to IVM, limiting the depth from which significant data can be obtained. Therefore, making quantitative measurements by IVM requires methods that use internal calibration, or alternatively, a completely different way of evaluating the signals...
April 20, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28434166/antibody-uptake-assay-in-the-embryonic-zebrafish-forebrain-to-study-notch-signaling-dynamics-in-neural-progenitor-cells-in-vivo
#16
Kai Tong, Mahendra Wagle, Su Guo
Stem cells can generate cell fate heterogeneity through asymmetric cell division (ACD). ACD derives from the asymmetric segregation of fate-determining molecules and/or organelles in the dividing cell. Radial glia in the embryonic zebrafish forebrain are an excellent model for studying the molecular mechanisms regulating ACD of stem cells in vertebrates, especially for live imaging concerning in vivo molecular and cellular dynamics. Due to the current difficulty in expressing fluorescent reporter-tagged proteins at physiological levels in zebrafish for live imaging, we have developed an antibody uptake assay to label proteins in live embryonic zebrafish forebrain with high specificity...
April 23, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28433823/simultaneous-monitoring-of-intra-and-extracellular-nitric-oxide-in-living-cells-by-means-of-dual-color-fluorescence-imaging
#17
Hui-Wen Yao, Jian-Bo Chen, Xiao-Feng Guo, Hong Wang
A dual-color fluorescence imaging method for simultaneous monitoring of intra- and extracellular nitric oxide (NO) was developed. Assisted by confocal laser scanning microscope, the intra- and extracellular NO can be successfully visualized by using two selected probes, 4,4-difluoro-8-(3,4-diaminophenyl)-3,5-bis(4-methoxyphenyl)-4-bora-3a,4a-diaza-s-indacene (p-MOPB) and disodium 2,6-disulfonate-1,3-dimethyl-5-hexadecyl-8-(3,4-diaminophenyl)-4,4'-difluoro-4-bora-3a,4a-diaza-s-indacene (DSDMHDAB), which display distinct membrane permeability and show different colors of fluorescence after reaction with NO...
April 19, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28432918/a-quinoline-based-cu-2-ion-complex-fluorescence-probe-for-selective-detection-of-inorganic-phosphate-anion-in-aqueous-solution-and-its-application-to-living-cells
#18
Yanpeng Dai, Peng Wang, Jiaxin Fu, Kun Yao, Kuoxi Xu, Xiaobin Pang
A quinaldine functionalized probe QP has been designed and synthesized. It exhibited selective turn-off fluorescence response toward Cu(2+) ion over most of the biologically important ions at physiological pH. The binding ratio of the probe QP and Cu(2+) ion was determined to be 1:1 through fluorescence titration, Job's plot and ESI-MS. The binding constant (K) of Cu(2+) to probe QP was found to be 2.12×10(4)M(-1). Further, the Cu(2+) ensemble of probe QP was found to respond H2PO4(-) and HPO4(2-) among other important biological anions via fluorescence turn-on response at physiological pH...
April 18, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28430668/a-turn-on-fluorescent-probe-for-endogenous-formaldehyde-in-the-endoplasmic-reticulum-of-living-cells
#19
Yonghe Tang, Yanyan Ma, An Xu, Gaoping Xu, Weiying Lin
As the simplest aldehyde compounds, formaldehyde (FA) is implicated in nervous system diseases and cancer. Endoplasmic reticulum is an organelle that plays important functions in living cells. Accordingly, the development of efficient methods for FA detection in the endoplasmic reticulum (ER) is of great biomedical importance. In this work, we developed the first ER-targeted fluorescent FA probe Na-FA-ER. The detection is based on the condensation reaction of the hydrazine group and FA to suppress the photo-induced electron transfer (PET) pathway, resulting in a fluorescence increase...
April 21, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28429463/global-mapping-of-protein-lipid-interactions-by-using-modified-choline-containing-phospholipids-metabolically-synthesized-in-live-cells
#20
Danyang Wang, Shubo Du, Amaury Cazenave-Gassiot, Jingyan Ge, Jun-Seok Lee, Markus R Wenk, Shao Q Yao
The protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein-lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein-phospholipid interactions have been successfully imaged in live cells...
April 21, 2017: Angewandte Chemie
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