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JOURNAL ARTICLE
Zahra Mahmoudi, Mojtaba Jahani, Reza Nekouian
CONTEXT: Most of the patients diagnosed with non-small cell lung cancer (NSCLC) are in their advanced stages and as a result might not be cured in spite of the advances in aimed therapy. In the recent years, the role of noncoding RNAs (ncRNAs) has been expanded to cancer as potential targets for RNA-based epigenetic therapies. Curcumin, as an active ingredient, is associated with epigenetic alterations, and it might modulate the expression of tumor suppressor and oncogenic microRNAs. MATERIALS AND METHODS: In this study, we investigated the RNA-based epigenetic effects of curcumin on NSCLC, and the effect of curcumin on A549 cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay...
October 1, 2023: Journal of Cancer Research and Therapeutics
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JOURNAL ARTICLE
Wenwu Kan, Linhui Gao, Jingnan Chen, Li Chen, Guojun Zhang, Bilie Hao, Min He, Xudong Chen, Cheng Wang
Glioblastoma (GBM) is the most common malignant brain tumor and has the poorest prognosis attributed to its chemoresistance to temozolomide (TMZ), the first-line drug for treating GBM. TMZ resistance represents a significant obstacle to successful GBM treatment, necessitating the development of new strategies to overcome this resistance and augment the chemosensitivity of GBM cells to TMZ. This study established a TMZ-resistant U251 (U251-TMZ) cell line by exposing it to increasing doses of TMZ in vitro. We focused on the DNA methyltransferase 3B (DNMT3B) gene, phosphorylated Akt (p-Akt), total Akt (t-Akt), phosphorylated PI3K (p-PI3K), and total PI3K (t-PI3K) protein expression...
February 17, 2024: Molecular Neurobiology
#23
JOURNAL ARTICLE
Junxiang Ji, Jianjian Cao, Peng Chen, Ru Huang, Shou-Dong Ye
Inhibition of protein Kinase C (PKC) efficiently promoted the self-renewal of embryonic stem cells (ESCs). However, information about the function of PKC inhibition remains lacking. Here, RNA- sequencing showed that the addition of Go6983 significantly inhibited the expression of de novo methyltransferases (Dnmt3a and Dnmt3b) and their regulator Dnmt3l, resulting in global hypomethylation of DNA in mouse ESCs. Mechanistically, PR domain-containing 14 (Prdm14), a site-specific transcriptional activator, partially contributed to Go6983-mediated repression of Dnmt3 genes...
February 1, 2024: Journal of Biological Chemistry
#24
JOURNAL ARTICLE
Junjiao Ping, Jing Wan, Jiali Luo, Baoguo Du, Xinxia Liu, Tingyun Jiang, Jie Zhang
OBJECTIVE: Schizophrenia belongs to a severe mental illness with complicated clinical presentations, an ill-defined pathogenesis, and no known cause. Many genetic studies imply that polygenic interaction is important in the development of schizophrenia. The main mechanism of the RELN-BDNF-CREB-DNMT signaling pathway in neurodevelopment involves RELN, brain-derived neurotrophic factor (BDNF), transcription factor cyclic adenosine monophosphate response element binding protein (CREB), DNA methyltransferase 1 (DNMT1), as well as DNA methyltransferase 3B (DNMT3B)...
January 31, 2024: International Journal of Developmental Neuroscience
#25
JOURNAL ARTICLE
Yang Li, Haley J Abel, Michelle Cai, Taylor A LaValle, Tiankai Yin, Nichole M Helton, Amanda M Smith, Christopher A Miller, Timothy J Ley
Here, we characterize the DNA methylation phenotypes of bone marrow cells from mice with hematopoietic deficiency of Dnmt3a or Dnmt3b (or both enzymes) or expressing the dominant-negative Dnmt3a R878H mutation [R882H in humans; the most common DNMT3A mutation found in acute myeloid leukemia (AML)]. Using these cells as substrates, we defined DNA remethylation after overexpressing wild-type (WT) DNMT3A1, DNMT3B1, DNMT3B3 (an inactive splice isoform of DNMT3B), or DNMT3L (a catalytically inactive "chaperone" for DNMT3A and DNMT3B in early embryogenesis)...
February 2, 2024: Science Advances
#26
JOURNAL ARTICLE
Francesca Taglini, Ioannis Kafetzopoulos, Willow Rolls, Kamila Irena Musialik, Heng Yang Lee, Yujie Zhang, Mattia Marenda, Lyndsay Kerr, Hannah Finan, Cristina Rubio-Ramon, Philippe Gautier, Hannah Wapenaar, Dhananjay Kumar, Hazel Davidson-Smith, Jimi Wills, Laura C Murphy, Ann Wheeler, Marcus D Wilson, Duncan Sproul
The correct establishment of DNA methylation patterns is vital for mammalian development and is achieved by the de novo DNA methyltransferases DNMT3A and DNMT3B. DNMT3B localises to H3K36me3 at actively transcribing gene bodies via its PWWP domain. It also functions at heterochromatin through an unknown recruitment mechanism. Here, we find that knockout of DNMT3B causes loss of methylation predominantly at H3K9me3-marked heterochromatin and that DNMT3B PWWP domain mutations or deletion result in striking increases of methylation in H3K9me3-marked heterochromatin...
January 30, 2024: EMBO Reports
#27
JOURNAL ARTICLE
Bingru Zhao, Langda Suo, Yujiang Wu, Tong Chen, Hanikezi Tulafu, Qingwei Lu, Wenna Liu, Abdul Sammad, Cuiling Wu, Xuefeng Fu
Tibetan cashmere goats are not only served as a valuable model for studying adaptation to hypoxia and high-altitude conditions but also playing a pivotal role in bolstering local economies through the provision of premium quality cashmere yarn. In this study, we performed an integration and network analysis of metabolomic, transcriptomic and proteomic to elucidate the role of differentially expressed genes, important metabolites, and relevant cellular and metabolic pathways between the fine (average 12.04 ± 0...
January 27, 2024: Genomics
#28
JOURNAL ARTICLE
Yuhong Ding, Akhilesh K Bajpai, Fengxia Wu, Weihua Lu, Lin Xu, Jiawei Mao, Qiang Li, Qi Pan, Lu Lu, Xinfeng Wang
Acute myeloid leukemia (AML) is a highly heterogeneous malignant disease of the blood cell. The current therapies for AML are unsatisfactory and the molecular mechanisms underlying AML are unclear. 5-methylcytosine (m5C) is an important posttranscriptional modification of mRNA, and is involved in the regulation of mRNA stability, translation, and other aspects of RNA metabolism. However, based on our knowledge of published literature, the role of the m5C regulators has not been explored in AML till date. In this study, we clarified the expression and gene variants of m5C regulators in AML and found that most m5C regulators were differentially expressed and correlated with disease prognosis...
January 25, 2024: Aging
#29
JOURNAL ARTICLE
Fatma Dogan, Nicholas R Forsyth
Telomere repeats protect linear chromosomes from degradation, and telomerase has a prominent role in their maintenance. Telomerase has telomere-independent effects on cell proliferation, DNA replication, differentiation, and tumorigenesis. TERT (telomerase reverse transcriptase enzyme), the catalytic subunit of telomerase, is required for enzyme activity. TERT promoter mutation and methylation are strongly associated with increased telomerase activation in cancer cells. TERT levels and telomerase activity are downregulated in stem cells during differentiation...
January 19, 2024: Biomolecules
#30
JOURNAL ARTICLE
Chantalle Moulton, Arianna Murri, Gianmarco Benotti, Cristina Fantini, Guglielmo Duranti, Roberta Ceci, Elisa Grazioli, Claudia Cerulli, Paolo Sgrò, Cristina Rossi, Stefano Magno, Luigi Di Luigi, Daniela Caporossi, Attilio Parisi, Ivan Dimauro
Most anticancer treatments act on oxidative-stress pathways by producing reactive oxygen species (ROS) to kill cancer cells, commonly resulting in consequential drug-induced systemic cytotoxicity. Physical activity (PA) has arisen as an integrative cancer therapy, having positive health effects, including in redox-homeostasis. Here, we investigated the impact of an online supervised PA program on promoter-specific DNA methylation, and corresponding gene expression/activity, in 3 antioxidants- (SOD1, SOD2, and CAT) and 3 breast cancer (BC)-related genes (BRCA1, L3MBTL1 and RASSF1A) in a population-based sample of women diagnosed with primary BC, undergoing medical treatment...
January 5, 2024: Redox Biology
#31
JOURNAL ARTICLE
Mohammad Khan, Kaitlyn Hearn, Christian Parry, Mudasir Rasid, Hassan Brim, Hassan Ashktorab, Bernard Kwabi-Addo
Prostate cancer is the most common cancer and the second leading cause of cancer deaths among men in the USA. Several studies have demonstrated the antitumor properties of saffron in different types of cancers, including prostate cancer. The oral administration of saffron extract has been reported to have antitumor effects on aggressive prostate-cancer-cell-line-derived xenografts in nude male mice. The objective of this study was to carry out in vitro studies of saffron-treated prostate cancer cells to ascertain the effects of saffron on key intermediates in prostate carcinogenesis...
December 28, 2023: Nutrients
#32
JOURNAL ARTICLE
Hyunyoung Yoon, Kyung Lib Jang
Hepatitis C virus (HCV) is constantly exposed to considerable oxidative stress, characterized by elevated levels of reactive oxygen species, including hydrogen peroxide (H2 O2 ), during acute and chronic infection in the hepatocytes of patients. However, the effect of oxidative stress on HCV replication is largely unknown. In the present study, we demonstrated that H2 O2 downregulated HCV Core levels to inhibit HCV replication. For this purpose, H2 O2 upregulated p53 levels, resulting in the downregulation of both the protein and enzyme activity levels of DNA methyltransferase 1 (DNMT1), DNMT3a, and DNMT3b, and activated the expression of E6-associated protein (E6AP) through promoter hypomethylation in the presence of HCV Core...
December 28, 2023: Cells
#33
JOURNAL ARTICLE
Samaneh Delavari, Seyed Erfan Rasouli, Saba Fekrvand, Zahra Chavoshzade, Seyed Alireza Mahdaviani, Paniz Shirmast, Samin Sharafian, Roya Sherkat, Tooba Momen, Soheila Aleyasin, Hamid Ahanchian, Mahnaz Sadeghi-Shabestari, Hossein Esmaeilzadeh, Sahar Barzamini, Fateme Tarighatmonfared, Helia Salehi, Marzie Esmaeili, Zahra Marzani, Nazanin Fathi, Farhad Abolnezhadian, Mina Kianmanesh Rad, Ali Saeedi-Boroujeni, Afshin Shirkani, Zahra Bagheri, Fereshte Salami, Tannaz Moeini Shad, Mahsa Yousefpour Marzbali, Hanieh Mojtahedi, Azadehsadat Razavi, Naeimeh Tavakolinia, Taher Cheraghi, Marzieh Tavakol, Alireza Shafiei, Nasrin Behniafard, Sare Sadat Ebrahimi, Najmeh Sepahi, Amirhossein Ghaneimoghadam, Arezou Rezaei, Arash Kalantari, Hassan Abolhassani, Nima Rezaei
BACKGROUND: Inborn errors of immunity (IEI) are a diverse range of genetic immune system illnesses affecting the innate and/or adaptive immune systems. Variable expressivity and incomplete penetrance have been reported in IEI patients with similar clinical diagnoses or even the same genetic mutation. METHODS: Among all recorded patients in the national IEI registry, 193 families with multiple cases have been recognized. Clinical, laboratory and genetic variability were compared between 451 patients with different IEI entities...
January 4, 2024: Clinical Immunology: the Official Journal of the Clinical Immunology Society
#34
JOURNAL ARTICLE
Yuhang Hu, Zhichao Lang, Xinmiao Li, Lifan Lin, Yifei Li, Rongrong Zhang, Jianjian Zheng, Zhengping Yu
BACKGROUND: Ginsenoside Rg3 (G-Rg3), extracted from Panax notoginseng, possesses hepatoprotective properties. Hepatic stellate cells (HSCs) activation is responsible for liver fibrosis. Recent studies have reported the suppressive effects of G-Rg3 on HSC activation and proliferation. Ferroptosis is a novel iron regulated cell death. ACSL4, a key indicator of ferroptosis, is commonly methylated in various diseases. PURPOSE: However, the role of ACSL4 methylation-mediated HSC ferroptosis in G-Rg3 inhibition of hepatic fibrosis needs to be explored...
December 16, 2023: Phytomedicine
#35
JOURNAL ARTICLE
Yeonhwa Song, Namjeong Kim, Jinyeong Heo, David Shum, Taemoo Heo, Haeng Ran Seo
Hepatocellular carcinoma (HCC) is a complex disease associated with a plethora of environmental and genetic/hereditary causative risk factors, more so than other oncological indications. Additionally, patients with HCC exhibit fibrosis, cirrhosis, and liver-related disease. This complicated etiology can affect the disease course and likely contributes to its poor prognosis. In this study, we aimed to improve HCC therapy by evaluating combination treatment using anti-cancer and anti-fibrosis drugs via identification of novel anti-fibrosis drugs...
January 2, 2024: Scientific Reports
#36
JOURNAL ARTICLE
Ryota Kajihara, Ryo Ezaki, Kennosuke Ichikawa, Tenkai Watanabe, Takumi Terada, Mei Matsuzaki, Hiroyuki Horiuchi
Activation of Wnt/β-catenin signaling supports the self-renewal of mouse embryonic stem cells. We aimed to understand the effects of Wnt signaling activation or inhibition on chicken embryonic stem cells (chESCs), as these effects are largely unknown. When the glycogen synthase kinase-3 β inhibitor CHIR99021-which activates Wnt signaling-was added to chESC cultures, the colony shape flattened, and the expression levels of pluripotency-related (NANOG, SOX2, SOX3, OCT4, LIN28A, DNMT3B, and PRDM14) and germ cell (CVH and DAZL) markers showed a decreasing trend, and the growth of chESCs was inhibited after approximately 7 d...
December 7, 2023: Poultry Science
#37
JOURNAL ARTICLE
Zhuang Wang, Bettina Heid, Jianlin He, Hehuang Xie, Christopher M Reilly, Rujuan Dai, S Ansar Ahmed
We previously demonstrated that the upregulation of microRNAs (miRNAs) at the genomic imprinted Dlk1-Dio3 locus in murine lupus is correlated with global DNA hypomethylation. We now report that the Dlk1-Dio3 genomic region in CD4+ T cells of MRL/lpr mice is hypomethylated, linking it to increased Dlk1-Dio3 miRNA expression. We evaluated the gene expression of methylating enzymes, DNA methyltransferases (DNMTs), and demethylating ten-eleven translocation proteins (TETs) to elucidate the molecular basis of DNA hypomethylation in lupus CD4+ T cells...
December 1, 2023: ImmunoHorizons
#38
JOURNAL ARTICLE
Udayadharshini Subaramaniyam, Divya Ramalingam, Ranjini Balan, Biswaranjan Paital, Pranati Sar, Nirmaladevi Ramalingam
Leukemia is a haematological malignancy affecting blood and bone marrow, ranking 10th among the other common cancers. DNA methylation is an epigenetic dysregulation that plays a critical role in leukemogenesis. DNA methyltransferases (DNMTs) such as DNMT1, DNMT3A and DNMT3B are the key enzymes catalysing DNA methylation. Inhibition of DNMT1 with secondary metabolites from medicinal plants helps reverse DNA methylation. The present study focuses on inhibiting DNMT1 protein (PDB ID: 3PTA) with annonaceous acetogenins through in-silico studies...
December 27, 2023: Journal of Biomolecular Structure & Dynamics
#39
JOURNAL ARTICLE
Yibing Liu, Lisui Bao, Sarah R Catalano, Xiaochen Zhu, Xiaoxu Li
High mortalities and highly variable results during the subsequent development of post-thaw larvae have been widely considered as key issues restricting the application of cryopreservation techniques to support genetic improvement programs and hatchery production in farmed marine bivalve species. To date, few studies have been undertaken to investigate the effects of cryodamage at the molecular level in bivalves. This study is the first to evaluate the effect of larval cryopreservation on the epigenetics of the resultant progenies of the Pacific oyster Crassostrea gigas ...
December 8, 2023: International Journal of Molecular Sciences
#40
JOURNAL ARTICLE
Liang Xu, Ming Cai
Immune rejection is a significant concern in organ transplantation, as it can lead to damage to and failure of the transplanted organ. To prevent or treat immune rejection, transplant recipients are commonly administered immunosuppressive drugs. Tacrolimus (FK506) is a widely used immunosuppressive drug in organ transplantation. The excessive formation of neutrophil extracellular traps (NETs) can contribute to inflammation and tissue damage. Although NETs play an antimicrobial role, their overproduction can be harmful...
November 25, 2023: Life
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