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https://www.readbyqxmd.com/read/28300471/decoupling-of-dna-methylation-and-activity-of-intergenic-line-1-promoters-in-colorectal-cancer
#1
Natasha Vafadar-Isfahani, Christina Parr, Lara E McMillan, Juliane Sanner, Zhao Yeo, Stephen Saddington, Oliver Peacock, Hazel A Cruickshanks, Richard R Meehan, Jonathan N Lund, Cristina Tufarelli
Hypomethylation of LINE-1 repeats in cancer has been proposed as the main mechanism behind their activation; this assumption, however, was based on findings from early studies that were biased towards young and transpositionally active elements. Here, we investigate the relationship between methylation of two intergenic, transpositionally inactive LINE-1 elements and expression of the LINE-1 chimeric transcript (LCT) 13 and LCT14 driven by their antisense promoters (L1-ASP). Our data from DNA modification, expression, and 5'RACE analyses suggest that colorectal cancer methylation in the regions analyzed is not always associated with LCT repression...
March 16, 2017: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/28278502/kaempferol-modulates-dna-methylation-and-downregulates-dnmt3b-in-bladder-cancer
#2
Wei Qiu, Jun Lin, Yichen Zhu, Jian Zhang, Liping Zeng, Ming Su, Ye Tian
BACKGROUND: Genomic DNA methylation plays an important role in both the occurrence and development of bladder cancer. Kaempferol (Kae), a natural flavonoid that is present in many fruits and vegetables, exhibits potent anti-cancer effects in bladder cancer. Similar to other flavonoids, Kae possesses a flavan nucleus in its structure. This structure was reported to inhibit DNA methylation by suppressing DNA methyltransferases (DNMTs). However, whether Kae can inhibit DNA methylation remains unclear...
March 8, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28270006/-effects-of-dietary-monosodium-glutamate-on-gene-expression
#3
Júlia Vanda Jurasek, László Bence Raposa, Andrea Gubicskóné Kisbenedek, Veronika Varga, Zoltán Szabó, Tímea Varjas
INTRODUCTION: Nowadays, the food industry more often uses different type of additives during the food production. AIM: Our aim was to examine the monosodium-glutamate's effect (in animal experiment) on DNA-methyltransferases in gene expression patterns of mRNA levels. MATERIALS AND METHOD: In the investigation we used 24 (n=24) CD1 type female mice. The animals were fed with different equivalent human doses of the tested substance. After autopsy, mRNA was isolated from different tissues (lung, liver, kidney, spleen)...
March 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/28267419/expanding-the-repertoire-of-deadenylases
#4
Ilias Skeparnias, Dimitrios Αnastasakis, Athanasios-Nasir Shaukat, Katerina Grafanaki, Constantinos Stathopoulos
Deadenylases belong to an expanding family of exoribonucleases involved mainly in mRNA stability and turnover, with the exception of PARN which has additional roles in the biogenesis of several important non-coding RNAs, including miRNAs and piRNAs. Recently, PARN in C. elegans and its homologue PNLDC1 in B. mori were reported as the elusive trimmers mediating piRNA biogenesis. In addition, characterization of mammalian PNLDC1 in comparison to PARN, showed that is specifically expressed in embryonic stem and germ cells, as well as during early embryo development...
March 7, 2017: RNA Biology
https://www.readbyqxmd.com/read/28263966/lncrna-meg3-downregulation-mediated-by-dnmt3b-contributes-to-nickel-malignant-transformation-of-human-bronchial-epithelial-cells-via-modulating-phlpp1-transcription-and-hif-1%C3%AE-translation
#5
C Zhou, C Huang, J Wang, H Huang, J Li, Q Xie, Y Liu, J Zhu, Y Li, D Zhang, Q Zhu, C Huang
Long noncoding RNAs (lncRNAs) are emerging as key factors in various fundamental cellular biological processes, and many of them are likely to have functional roles in tumorigenesis. Maternally expressed gene 3 (MEG3) is an imprinted gene located at 14q32 that encodes a lncRNA, and the decreased MEG3 expression has been reported in multiple cancer tissues. However, nothing is known about the alteration and role of MEG3 in environmental carcinogen-induced lung tumorigenesis. Our present study, for the first time to the best of our knowledge, discovered that environmental carcinogen nickel exposure led to MEG3 downregulation, consequently initiating c-Jun-mediated PHLPP1 transcriptional inhibition and hypoxia-inducible factor-1α (HIF-1α) protein translation upregulation, in turn resulting in malignant transformation of human bronchial epithelial cells...
March 6, 2017: Oncogene
https://www.readbyqxmd.com/read/28260016/effects-of-r-type-and-s-type-ginsenoside-rg3-on-dna-methylation-in-human-hepatocarcinoma-cells
#6
Siying Teng, Yi Wang, Pingya Li, Jinhua Liu, Anhui Wei, Haotian Wang, Xiangkun Meng, Di Pan, Xinmin Zhang
Ginsenoside Rg3, a bioactive constituent isolated from Panax ginseng, exhibits antitumorigenic, antioxidative, antiangiogenic, neuroprotective and other biological activities are associated with the regulation of multiple genes. DNA methylation patterns, particularly those in the promoter region, affect gene expression, and DNA methylation is catalyzed by DNA methylases. However, whether ginsenoside Rg3 affects DNA methylation is unknown. High performance liquid chromatography assay, MspI/HpaII polymerase chain reaction (PCR) and reverse transcription‑quantitative PCR were performed to assess DNA methylation...
February 28, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28258101/dnmt3b-mediated-gene-body-methylation-suppresses-aberrant-transcription
#7
(no author information available yet)
DNMT3B methylates intragenic DNA to prevent spurious transcription from cryptic intragenic sites.
March 3, 2017: Cancer Discovery
https://www.readbyqxmd.com/read/28257867/cobalamin-supplementation-during-in%C3%A2-vitro-maturation-improves-developmental-competence-of-sheep-oocytes
#8
Federica Zacchini, Paola Toschi, Grazyna Ewa Ptak
Pregnancies obtained by Assisted Reproductive Technologies are at higher risk of miscarriage than those obtained naturally. Previously, we reported impaired placental vascular development of in vitro produced (IVP) sheep embryos and defective DNA methylation in the placentae of those embryos. One reason behind these observed defects may be an impaired One Carbon Metabolism (OCM) The present study was performed to test the hypothesis that Cobalamin (Vitamin B12, an important OCM co-factor) supplementation during IVM corrects DNA methylation of IVP embryos and, consequently, ameliorates placental vasculogenesis...
April 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28225755/intragenic-dna-methylation-prevents-spurious-transcription-initiation
#9
Francesco Neri, Stefania Rapelli, Anna Krepelova, Danny Incarnato, Caterina Parlato, Giulia Basile, Mara Maldotti, Francesca Anselmi, Salvatore Oliviero
In mammals, DNA methylation occurs mainly at CpG dinucleotides. Methylation of the promoter suppresses gene expression, but the functional role of gene-body DNA methylation in highly expressed genes has yet to be clarified. Here we show that, in mouse embryonic stem cells, Dnmt3b-dependent intragenic DNA methylation protects the gene body from spurious RNA polymerase II entry and cryptic transcription initiation. Using different genome-wide approaches, we demonstrate that this Dnmt3b function is dependent on its enzymatic activity and recruitment to the gene body by H3K36me3...
February 22, 2017: Nature
https://www.readbyqxmd.com/read/28220825/notch1-promoted-trpa1-expression-in-erythroleukemic-cells-suppresses-erythroid-but-enhances-megakaryocyte-differentiation
#10
Ji-Lin Chen, Yueh-Hsin Ping, Min-Jen Tseng, Yuan-I Chang, Hsin-Chen Lee, Rong-Hong Hsieh, Tien-Shun Yeh
The Notch1 pathway plays important roles in modulating erythroid and megakaryocyte differentiation. To screen the Notch1-related genes that regulate differentiation fate of K562 and HEL cells, the expression of transient receptor potential ankyrin 1 (TRPA1) was induced by Notch1 receptor intracellular domain (N1IC), the activated form of Notch1 receptor. N1IC and v-ets erythroblastosis virus E26 oncogene homolog 1 (Ets-1) bound to TRPA1 promoter region to regulate transcription in K562 cells. Transactivation of TRPA1 promoter by N1IC depended on the methylation status of TRPA1 promoter...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220295/dnmt3b-579-g-t-promoter-polymorphism-and-the-risk-of-gastric-cancer-in-the-west-of-iran
#11
Kulsom Ahmadi, Azam Soleimani, Shiva Irani, Aliasghar Kiani, Kourosh Ghanadi, Zahra Noormohamadi, Foroozan Sakinejad
BACKGROUND: Many studies have suggested that modulation of DNMT3B function caused by single nucleotide polymorphisms of the DNMT3B promoter region may underlie the susceptibility to various cancers such as tumors of the digestive system. The aim of this study was to investigate the effect of -579 G>T polymorphism in the promoter of the DNMT3B gene on risk of gastric cancer in a population from West Iran. PATIENTS AND METHODS: We conducted a case-control study in 100 gastric cancer patients and 112 cancer-free controls to assess the correlation between DNMT3B -579 G>T (rs1569686) polymorphism and the risk of gastric cancer...
February 21, 2017: Journal of Gastrointestinal Cancer
https://www.readbyqxmd.com/read/28220037/differential-expression-and-clinical-significance-of-dna-methyltransferase-3b-dnmt3b-phosphatase-and-tensin-homolog-pten-and-human-mutl-homologs-1-hmlh1-in-endometrial-carcinomas
#12
Wenting Li, Ying Wang, Xinzhi Fang, Mei Zhou, Yiqun Li, Ying Dong, Ruozheng Wang
BACKGROUND The aim of this study was to investigate the expression and the clinicopathologic significance of DNA methyltransferase 3B (DNMT3B), phosphatase and tensin homolog (PTEN) and human MutL homologs 1 (hMLH1) in endometrial carcinomas between Han and Uygur women in Xinjiang. MATERIAL AND METHODS The expression of DNMT3B, PTEN, and hMLH1 in endometrial carcinomas were assessed by immunohistochemistry, followed by an analysis of their relationship to clinical-pathological features and prognosis. RESULTS There were a 61...
February 21, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28217009/meta-analysis-reveals-no-association-of-dnmt3b-149-c-t-gene-polymorphism-with-overall-cancer-risk
#13
Raju Kumar Mandal, Shafiul Haque, Mohd Wahid, Arshad Jawed, Naseem Akhter, Md Ekhlaque Ahmed Khan, Aditya Kumar Panda, Mohammed Yahya Areeshi, Sajad Ahmad Dar
BACKGROUND: DNA methyltransferase-3B (DNMT3B) plays a key role in establishment and maintenance of genomic methylation patterns. Polymorphism in promoter region -149 C>T (C46359T) of DNMT3B gene may alter DNMT3B activity which leads to increased susceptibility to cancer. Inconsistent results regarding this have been reported in a number of studies. OBJECTIVE: To carry out a meta-analysis of the studies reported to assess the precise relationship between the DNMT3B -149 C>T polymorphism and the overall cancer risk...
December 2016: Current Genomics
https://www.readbyqxmd.com/read/28215635/mechanical-stimulation-promote-the-osteogenic-differentiation-of-bone-marrow-stromal-cells-through-epigenetic-regulation-of-sonic-hedgehog
#14
Chuandong Wang, Shengzhou Shan, Chenglong Wang, Jing Wang, Jiao Li, Guoli Hu, Kerong Dai, Qingfeng Li, Xiaoling Zhang
Mechanical unloading leads to bone loss and disuse osteoporosis partly due to impaired osteoblastogenesis of bone marrow stromal cells (BMSCs). However, the underlying molecular mechanisms of this phenomenon are not fully understood. In this study, we demonstrated that cyclic mechanical stretch (CMS) promotes osteoblastogenesis of BMSCs both in vivo and in vitro. Besides, we found that Hedgehog (Hh) signaling pathway was activated in this process. Inhibition of which by either knockdown of Sonic hedgehog (Shh) or treating BMSCs with Hh inhibitors attenuated the osteogenic effect of CMS on BMSCs, suggesting that Hh signaling pathway acts as an endogenous mediator of mechanical stimuli on BMSCs...
March 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28192769/disorganization-at-the-stage-of-schizophrenia-clinical-outcome-clinical-biological-study
#15
A Nestsiarovich, V Obyedkov, H Kandratsenka, M Siniauskaya, I Goloenko, N Waszkiewicz
BACKGROUND: According to the multidimensional model of schizophrenia, three basic psychopathological dimensions constitute its clinical structure: positive symptoms, negative symptoms and disorganization. The latter one is the newest and the least studied. Our aim was to discriminate disorganization in schizophrenia clinical picture and to identify its distinctive biological and socio-psychological particularities and associated genetic and environmental factors. METHODS: We used SAPS/SANS psychometrical scales, scales for the assessment of patient's compliance, insight, social functioning, life quality...
December 30, 2016: European Psychiatry: the Journal of the Association of European Psychiatrists
https://www.readbyqxmd.com/read/28177119/downregulation-of-methyltransferase-dnmt2-results-in-condition-dependent-telomere-shortening-and-senescence-or-apoptosis-in-mouse-fibroblasts
#16
Anna Lewinska, Jagoda Adamczyk-Grochala, Ewa Kwasniewicz, Maciej Wnuk
Dnmt2 is a highly conserved methyltransferase of uncertain biological function(s). As Dnmt2 was considered as a driver of fruit fly longevity and a modulator of stress response, we decided to evaluate the role of Dnmt2 during stress-induced premature senescence in NIH3T3 mouse fibroblasts. Stable knockdown of Dnmt2 resulted in hydrogen peroxide-mediated sensitivity and apoptosis, whereas in the control conditions, senescence was induced. Cellular senescence was accompanied by elevated levels of p53 and p21, decreased telomere length and telomerase activity, increased production of reactive oxygen species and protein carbonylation, and DNA damage...
February 8, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28161170/maternal-supplementation-with-rumen-protected-methionine-increases-prepartal-plasma-methionine-concentration-and-alters-hepatic-mrna-abundance-of-1-carbon-methionine-and-transsulfuration-pathways-in-neonatal-holstein-calves
#17
C B Jacometo, Z Zhou, D Luchini, M N Corrêa, J J Loor
An important mechanism of nutritional "programming" induced by supplementation with methyl donors during pregnancy is the alteration of mRNA abundance in the offspring. We investigated the effects of rumen-protected Met (RPM) on abundance of 17 genes in the 1-carbon, Met, and transsulfuration pathways in calf liver from cows fed the same basal diet without (control, CON) or with RPM at 0.08% of diet dry matter/d (MET) from -21 through +30 d around calving. Biopsies (n = 8 calves per diet) were harvested on d 4, 14, 28, and 50 of age...
February 1, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28160561/an-association-between-overexpression-of-dna-methyltransferase-3b4-and-clear-cell-renal-cell-carcinoma
#18
You Liu, Liantao Sun, Peter Fong, Jie Yang, Zhuxia Zhang, Shuihui Yin, Shuyuan Jiang, Xiaolei Liu, Hongge Ju, Lihua Huang, Jing Bai, Kerui Gong, Shaochun Yan, Chunyang Zhang, Guo Shao
It is well known that abnormal DNA methylations occur frequently in kidney cancer. However, it remains unclear exactly which types of DNA methyltransferases (DNMT) contribute to the pathologies of kidney cancers. In order to determine the functions of DNA methyltransferase in kidney tumorigenesis on the molecular level, we examined the mRNA expression levels of DNMT1, DNMT3A, DNMT3B, and DNMT3B variants in renal cell carcinoma tissue. Both mRNA and protein levels of DNMT3B4, a splice variant of DNMT3B, were increased in renal cell carcinoma tissue compared with adjacent control tissues...
February 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28128455/expanding-the-mutation-spectrum-in-icf-syndrome-evidence-for-a-gender-bias-in-icf2
#19
Marlinde L van Boogaard, Peter E Thijssen, Caner Aytekin, Francesco Licciardi, Ayça A Kıykım, Lucia Spossito, Virgil A S H Dalm, Gertjan J Driessen, Rogier Kersseboom, Femke de Vries, Monique M van Ostaijen-Ten Dam, Aydan Ikinciogullari, Figen Dogu, Matias Oleastro, Edgardo Bailardo, Lucia Daxinger, Ercan Nain, Safa Baris, Maarten J D van Tol, Corry Weemaes, Silvère M van der Maarel
Immunodeficiency, centromere instability and facial anomalies (ICF) syndrome is a rare, genetically heterogeneous, autosomal recessive disorder. Patients suffer from recurrent infections caused by reduced levels or absence of serum immunoglobulins. Genetically, four subtypes of ICF syndrome have been identified to date: ICF1 (DNMT3B mutations), ICF2 (ZBTB24 mutations), ICF3 (CDCA7 mutations) and ICF4 (HELLS mutations). In this study we describe seven ICF1 patients and six novel missense mutations in DNMT3B, affecting highly conserved residues in the catalytic domain...
January 27, 2017: Clinical Genetics
https://www.readbyqxmd.com/read/28127428/dna-methyltransferases-and-their-roles-in-tumorigenesis
#20
REVIEW
Wu Zhang, Jie Xu
DNA methylation plays an important role in gene expression, chromatin stability, and genetic imprinting. In mammals, DNA methylation patterns are written and regulated by DNA methyltransferases (DNMTs), including DNMT1, DNMT3A and DNMT3B. Recent emerging evidence shows that defects in DNMTs are involved in tumor transformation and progression, thus indicating that epigenetic disruptions caused by DNMT abnormalities are associated with tumorigenesis. Herein, we review the latest findings related to DNMT alterations in cancer cells and discuss the contributions of these effects to oncogenic phenotypes...
2017: Biomarker Research
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