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DNMT3B

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https://www.readbyqxmd.com/read/29449904/expression-profiling-of-chromatin-modifying-enzymes-and-global-dna-methylation-in-cd4-t-cells-from-patients-with-chronic-hiv-infection-at-different-hiv-control-and-progression-states
#1
Roberta Nicoleta Bogoi, Alicia de Pablo, Eulalia Valencia, Luz Martín-Carbonero, Victoria Moreno, Helem Haydee Vilchez-Rueda, Victor Asensi, Rosa Rodriguez, Victor Toledano, Berta Rodés
Background: Integration of human immunodeficiency virus type 1 (HIV-1) into the host genome causes global disruption of the chromatin environment. The abundance level of various chromatin-modifying enzymes produces these alterations and affects both the provirus and cellular gene expression. Here, we investigated potential changes in enzyme expression and global DNA methylation in chronically infected individuals with HIV-1 and compared these changes with non-HIV infected individuals...
2018: Clinical Epigenetics
https://www.readbyqxmd.com/read/29449903/specific-or-not-specific-recruitment-of-dnmts-for-dna-methylation-an-epigenetic-dilemma
#2
REVIEW
Eric Hervouet, Paul Peixoto, Régis Delage-Mourroux, Michaël Boyer-Guittaut, Pierre-François Cartron
Our current view of DNA methylation processes is strongly moving: First, even if it was generally admitted that DNMT3A and DNMT3B are associated with de novo methylation and DNMT1 is associated with inheritance DNA methylation, these distinctions are now not so clear. Secondly, since one decade, many partners of DNMTs have been involved in both the regulation of DNA methylation activity and DNMT recruitment on DNA. The high diversity of interactions and the combination of these interactions let us to subclass the different DNMT-including complexes...
2018: Clinical Epigenetics
https://www.readbyqxmd.com/read/29439669/a-novel-mir-375-hoxb3-cdca3-dnmt3b-regulatory-circuitry-contributes-to-leukemogenesis-in-acute-myeloid-leukemia
#3
Laixi Bi, Bin Zhou, Haiying Li, Licai He, Chunjing Wang, Zhonggai Wang, Liqing Zhu, Mengqian Chen, Shenmeng Gao
BACKGROUND: Acute myeloid leukemia (AML) is a heterogeneous group of hematopoietic malignancies due to sophisticated genetic mutations and epigenetic dysregulation. MicroRNAs (miRNAs), a class of small non-coding RNAs, are important regulators of gene expression in all biological processes, including leukemogenesis. Recently, miR-375 has been reported to be a suppressive miRNA in multiple types of cancers, but its underlying anti-leukemia activity in AML is largely unknown. METHODS: Quantitative reverse transcriptase PCR (qRT-PCR) was used to measure the expression of miR-375 and HOXB3 in leukemic cells and normal controls...
February 13, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29432024/evaluation-of-muc-1-aptamer-functionalized-hybrid-nanoparticles-for-targeted-delivery-of-mirna-29b-to-non-small-cell-lung-cancer
#4
Maryna Perepelyuk, Koita Sacko, Karthik Thangavel, Sunday A Shoyele
The objective of this study was to evaluate the therapeutic efficacy and pharmacokinetic study of mucin1-aptamer functionalized miRNA-29b-loaded hybrid nanoparticles (MAFMILHN) in lung tumor-bearing SCID mice. MAFMILHN were manufactured using an iso-electric point based nanotechnology. They were then fully characterized for particle size, loading capacity, Zeta potential and encapsulation efficiency. The ability of MAFMILHN to downregulate oncoprotein DNMT3B both at the cellular level and in vivo was monitored using western blot, while the effect of the downregulation of DNMT3B on tumor growth was assessed using bioluminescence...
February 12, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29425759/expression-of-proteins-upregulated-in-hepatocellular-carcinoma-in-patients-with-alcoholic-hepatitis-ah-compared-to-non-alcoholic-steatohepatitis-nash-an-immunohistochemical-analysis-of-candidate-proteins
#5
Jiajie George Lu, Luan Nguyen, Sara Samadzadeh, Maryam Masouminia, Alejandro Mendoza, Owen Sweeney, Brittany Tillman, Nikoo Afifyan, Timothy Morgan, Barbara A French, Samuel W French
Both non-alcoholic steatohepatitis (NASH) and alcoholic hepatitis (AH) can lead to cirrhosis and hepatocellular carcinoma. However, the rate of progression to cirrhosis and tumorigenesis in AH is greater than that in NASH. We asked whether there are differences between the two conditions in the expression levels of proteins involved in the pathogenesis of hepatocellular carcinoma. The proteins tested were presented at the 2017 American Association for the Study of Liver Diseases (AASLD) Liver Meeting as overexpressed in hepatocellular carcinoma: KLF4, SCL19A1, FANCG, HRH-1, DNMT1, DNMT3B, TNFR2, DUSP4, EGFR, Integrin α6, HDACII, PDE3A, BCL-XL, and MTCO2...
February 6, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29422991/preeclampsia-is-associated-with-hypermethylation-of-igf-1-promoter-mediated-by-dnmt1
#6
Min Ma, Qiong-Jie Zhou, Yu Xiong, Bin Li, Xiao-Tian Li
Previous studies have demonstrated a dynamic epigenetic regulation of genes expression in placenta trophoblasts and a dynamic imbalance of DNA methylation and hydroxymethylation. Reduced IGF-1 has been observed in preeclampsia. This study was to investigate the interactive roles between IGF-1 and the global DNA methylation/hydroxymethylation, and the status of DNA methylation/hydroxymethylation and associated enzymes such as DNMTs and TETs in peeeclamptic placentas and hypoxic trophoblasts. It was found that IGF-1 was decreased in preeclamptic placentas and hypoxic trophoblasts when compared to the control group using immunohistochemisty, western blot, qRT-PCR and ELISA...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/29416619/the-inhibitory-activity-of-gallic-acid-against-dna-methylation-application-of-gallic-acid-on-epigenetic-therapy-of-human-cancers
#7
Yui-Ping Weng, Pin-Feng Hung, Wen-Yen Ku, Chang-Yuan Chang, Bo-Han Wu, Ming-Han Wu, Jau-Ying Yao, Ji-Rui Yang, Chia-Huei Lee
Epigenome aberrations have been observed in tobacco-associated human malignancies. (-)-epigallocatechin-3-gallate (EGCG) has been proven to modulate gene expression by targeting DNA methyltransferases (DNMTs) through a proposed mechanism involving the gallate moiety of EGCG. We show that gallic acid (GA) changes the methylome of lung cancer and pre-malignant oral cell lines and markedly reduces both nuclear and cytoplasmic DNMT1 and DNMT3B within 1 week. GA exhibits stronger cytotoxicity against the lung cancer cell line H1299 than EGCG...
January 2, 2018: Oncotarget
https://www.readbyqxmd.com/read/29414941/structural-basis-for-dnmt3a-mediated-de-novo-dna-methylation
#8
Zhi-Min Zhang, Rui Lu, Pengcheng Wang, Yang Yu, Dongliang Chen, Linfeng Gao, Shuo Liu, Debin Ji, Scott B Rothbart, Yinsheng Wang, Gang Greg Wang, Jikui Song
DNA methylation by de novo DNA methyltransferases 3A (DNMT3A) and 3B (DNMT3B) at cytosines is essential for genome regulation and development. Dysregulation of this process is implicated in various diseases, notably cancer. However, the mechanisms underlying DNMT3 substrate recognition and enzymatic specificity remain elusive. Here we report a 2.65-ångström crystal structure of the DNMT3A-DNMT3L-DNA complex in which two DNMT3A monomers simultaneously attack two cytosine-phosphate-guanine (CpG) dinucleotides, with the target sites separated by 14 base pairs within the same DNA duplex...
February 7, 2018: Nature
https://www.readbyqxmd.com/read/29402602/genotype-and-phenotype-analysis-of-43-iranian-facioscapulohumeral-muscular-dystrophy-patients-evidence-for-anticipation
#9
Afagh Alavi, Sara Esmaeili, Shahriar Nafissi, Kimia Kahrizi, Hossein Najmabadi
Facioscapulohumeral muscular dystrophy (FSHD) is the third most common hereditary myopathy (prevalence 1/8300-1/20,000). It is typically characterized by progressive weakness of facial, scapular and humeral muscles. Pelvic, abdominal and lower limbs muscles may eventually be affected. FSHD is classified into two subgroups, FSHD1 and FSHD2. FSHD1 is due to a reduction in the copy number of D4Z4 macrosatellites on chromosome 4q35 (11-100 repeats in normal individuals and 1-10 repeats in patients), and FSHD2 is caused by mutations in SMCHD1 or DNMT3B...
January 12, 2018: Neuromuscular Disorders: NMD
https://www.readbyqxmd.com/read/29396184/reduced-self-diploidization-and-improved-survival-of-semi-cloned-mice-produced-from-androgenetic-haploid-embryonic-stem-cells-through-overexpression-of-dnmt3b
#10
Wenteng He, Xiaobai Zhang, Yalin Zhang, Weisheng Zheng, Zeyu Xiong, Xinjie Hu, Mingzhu Wang, Linfeng Zhang, Kun Zhao, Zhibin Qiao, Weiyi Lai, Cong Lv, Xiaochen Kou, Yanhong Zhao, Jiqing Yin, Wenqiang Liu, Yonghua Jiang, Mo Chen, Ruimin Xu, Rongrong Le, Chong Li, Hong Wang, Xiaoping Wan, Hailin Wang, Zhiming Han, Cizhong Jiang, Shaorong Gao, Jiayu Chen
Androgenetic haploid embryonic stem cells (AG-haESCs) hold great promise for exploring gene functions and generating gene-edited semi-cloned (SC) mice. However, the high incidence of self-diploidization and low efficiency of SC mouse production are major obstacles preventing widespread use of these cells. Moreover, although SC mice generation could be greatly improved by knocking out the differentially methylated regions of two imprinted genes, 50% of the SC mice did not survive into adulthood. Here, we found that the genome-wide DNA methylation level in AG-haESCs is extremely low...
February 13, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29383100/sirt1-regulates-mxd1-during-malignant-melanoma-progression
#11
Fabiana M Meliso, Danilo Micali, Camila T Silva, Thaís S Sabedot, Simon G Coetzee, Adrian Koch, Fabian B Fahlbusch, Houtan Noushmehr, Regine Schneider-Stock, Miriam G Jasiulionis
In a murine melanoma model, malignant transformation promoted by a sustained stress condition was causally related to increased levels of reactive oxygen species resulting in DNA damage and massive epigenetic alterations. Since the chromatin modifier Sirtuin-1 (SIRT1) is a protein attracted to double-stranded DNA break (DSB) sites and can recruit other components of the epigenetic machinery, we aimed to define the role of SIRT1 in melanomagenesis through our melanoma model. The DNA damage marker, γH2AX was found increased in melanocytes after 24 hours of deadhesion, accompanied by increased SIRT1 expression and decreased levels of its target, H4K16ac...
December 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29379364/dna-cytosine-5-methyltransferase-3b-dnmt-3b-polymorphism-and-risk-of-down-syndrome-offspring
#12
Cláudia Melo de Moura, Pedro Ribeiro Bastos, Julyana S V Ribeiro, Márcia Gonçalves Ribeiro, Márcia Rodrigues Amorim, Marcelo Aguiar Costa-Lima
Down syndrome (DS) is the most common form of human genetic mental retardation. Several polymorphisms in genes coding folic acid cycle enzymes have been associated to the risk of bearing a DS child; however, the results are controversial. S-adenosyl-l-methionine (SAM) is an important intermediate of folic acid pathway and acts as methyl donor and substrate for DNA (cytosine-5)-methyltransferase 3B (DNMT3B - EC 2.1.1.37) de novo methylation processes during embryogenesis. Recent studies suggest that a functional polymorphism of DNMT 3B in maternal genotype may be associated with a decreased risk of having a DS child...
January 2018: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/29378471/nanaomycin-a-treatment-promotes-hepatoblast-differentiation-from-human-induced-pluripotent-stem-cells
#13
Soichiro Nakamae, Yukiko Toba, Kazuo Takayama, Fuminori Sakurai, Hiroyuki Mizuguchi
Human induced pluripotent stem cell-derived hepatocyte-like cells are expected to be utilized in pharmaceutical research, including drug screening. However, the hepatocyte functions of the hepatocyte-like cells are still lower than those of human hepatocytes. Therefore, we attempted to improve the hepatocyte differentiation method by modulating the DNA epigenetic status. We first examined the expression profiles of the maintenance DNA methyltransferase (DNMT) 1 and the de novo DNA methyltransferases DNMT3A and DNMT3B, all of which are essential for mammalian development...
January 29, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29378290/antidepressant-administration-modulates-stress-induced-dna-methylation-and-dna-methyltransferase-expression-in-rat-prefrontal-cortex-and-hippocampus
#14
Amanda J Sales, Sâmia R L Joca
Stress and antidepressant treatment can modulate DNA methylation in promoter region of genes related to neuroplasticity and mood regulation, thus implicating this epigenetic mechanism in depression neurobiology and treatment. Accordingly, systemic administration of DNA methyltransferase (DNMT) inhibitors induces antidepressant-like effects in rodents. DNA methylation is conveyed by DNMT 1, 3a and 3b isoforms, which are differentially expressed in the brain. In order to investigate if the behavioral effects of antidepressants could be associated with changes in DNA methylation and DNMT expression, we investigated the effects induced by acute and repeated antidepressant treatment on DNA methylation and DNMT expression (1, 3a and 3b isoforms) in different brain regions of rats exposed to a stress model of depression, the learned helplessness (LH)...
January 26, 2018: Behavioural Brain Research
https://www.readbyqxmd.com/read/29364483/regulation-of-dna-methylation-and-tumor-suppression-gene-expression-by-mir-29b-in-leukemia-patients-and-related-mechanisms
#15
Y-L Chen, Z-X Zhang, L-H Shou, J-Y Di
OBJECTIVE: Leukemia is characterized as a kind of malignant clonal disease in hematological stem cells. The study showed an abnormal level of DNA methylation in leukemia cells, which further led to an abnormal expression of hematological genes. This study investigated the role of miR-29b on the modulation of DNA methylation and tumor suppressor gene expression in leukemia patients. PATIENTS AND METHODS: A total of 21 leukemia patients were recruited for the collection of monocytes...
January 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29363667/curcumol-controls-choriocarcinoma-stem-like-cells-self-renewal-via-repression-of-dna-methyltransferase-dnmt-and-histone-deacetylase-hdac-mediated-epigenetic-regulation
#16
Zheng Peng, Wenjun Zhou, Chun Zhang, Huining Liu, Yi Zhang
BACKGROUND Cancer stem cells (CSCs), in choriocarcinoma and other carcinomas, possess the ability of self-renewal and multilineage differentiation potential. We previous isolated choriocarcinoma cancer stem-like cells (CSLCs), which hold the stemness characteristics of CSCs. Epigenetic modifications have emerged as drivers in tumorigenesis, but the mechanisms of CSCs are largely unknown, and new drug therapies are needed to break the persistence of CSCs. MATERIAL AND METHODS Quantitative real-time PCR (qRT-PCR) and Western blot analysis were performed to detect the expression of DNMTs, HDACs, and stemness-genes...
January 24, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29361408/supplementing-in-vitro-embryo-production-media-by-nppc-and-sildenafil-affect-the-cytoplasmic-lipid-content-and-gene-expression-of-bovine-cumulus-oocyte-complexes-and-embryos
#17
Ramon Cesar Botigelli, Eduardo Montanari Razza, Elisa Mariano Pioltine, Patricia Kubo Fontes, Kátia Regina Lancellotti Schwarz, Cláudia Lima Verde Leal, Marcelo Fábio Gouveia Nogueira
In our study, we added natriuretic peptide type C (NPPC) and/or sildenafil during in vitro maturation (IVM) of bovine cumulus-oocyte complexes (COCs) followed by in vitro culture (IVC) of embryos with or without sildenafil. We evaluated the effects on the lipid content (LC) of oocytes and embryos and also verified the expression of 96 transcripts related to competence in matured COCs and 96 transcripts related to embryo quality in blastocysts. After IVM, LC was decreased in oocytes by NPPC while sildenafil did not affect LC in oocytes...
January 17, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29361151/chemoprevention-of-colorectal-cancer-by-black-raspberry-anthocyanins-involved-the-modulation-of-gut-microbiota-and-sfrp2-demethylation
#18
Lili Chen, Bowen Jiang, Chunge Zhong, Jun Guo, Lihao Zhang, Teng Mu, Qiuhua Zhang, Xiuli Bi
Freeze-dried black raspberry powder is considered as a potential cancer chemopreventive agent. In this study, we fed AOM/DSS treated C57BL/6J mice with diet containing black raspberry anthocyanins for 12 weeks and this led to a reduction in colon carcinogenesis. These animals had consistently lower tumor multiplicity compared to AOM/DSS-treated mice not receiving BRB anthocyanins. In AOM/DSS-treated mice, the number of pathogenic bacteria, including Desulfovibriosp and Enterococcus spp, were increased significantly, whereas probiotics such as Eubacterium rectale, Faecalibacterium prausnitzii and Lactobacillus were dramatically decreased, but BRB anthocyanins supplement could reverse this imbalance in gut microbiota...
January 19, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/29353135/copper-induces-expression-and-methylation-changes-of-early-development-genes-in-crassostrea-gigas-embryos
#19
Rossana Sussarellu, Morgane Lebreton, Julien Rouxel, Farida Akcha, Guillaume Rivière
Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development...
January 3, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29344166/identification-and-functional-analysis-of-risk-related-micrornas-for-the-prognosis-of-patients-with-bladder-urothelial-carcinoma
#20
Ji Gao, Hongyan Li, Lei Liu, Lide Song, Yanting Lv, Yuping Han
The aim of the present study was to investigate risk-related microRNAs (miRs) for bladder urothelial carcinoma (BUC) prognosis. Clinical and microRNA expression data downloaded from the Cancer Genome Atlas were utilized for survival analysis. Risk factor estimation was performed using Cox's proportional regression analysis. A microRNA-regulated target gene network was constructed and presented using Cytoscape. In addition, the Database for Annotation, Visualization and Integrated Discovery was used for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment, followed by protein-protein interaction (PPI) network analysis...
December 2017: Oncology Letters
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