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https://www.readbyqxmd.com/read/28110489/video-assisted-laser-resection-of-lung-metastases-feasibility-of-a-new-surgical-technique
#1
Christian Meyer, Detlef Bartsch, Nikolas Mirow, Andreas Kirschbaum
Background Our pilot study describes our initial experience to do a laser resection of lung metastases under video-assisted thoracoscopic control via a minithoracotomy. With this approach, if needed, mediastinal lymphadenectomy is also possible. Methods In this study, 15 patients (11 men and 4 women, mean age: 60 years) with resectable lung metastases of different solid primary tumors (colorectal cancer in seven patients, melanoma in three patients, renal cell carcinoma in two patients, and one each with oropharyngeal cancer, breast cancer, and seminoma) were included...
January 22, 2017: Thoracic and Cardiovascular Surgeon
https://www.readbyqxmd.com/read/28110456/facial-skin-blood-flow-responses-during-exposures-to-emotionally-charged-movies
#2
Kanji Matsukawa, Kana Endo, Kei Ishii, Momoka Ito, Nan Liang
The changes in regional facial skin blood flow and vascular conductance have been assessed for the first time with noninvasive two-dimensional laser speckle flowmetry during audiovisually elicited emotional challenges for 2 min (comedy, landscape, and horror movie) in 12 subjects. Limb skin blood flow and vascular conductance and systemic cardiovascular variables were simultaneously measured. The extents of pleasantness and consciousness for each emotional stimulus were estimated by the subjective rating from -5 (the most unpleasant; the most unconscious) to +5 (the most pleasant; the most conscious)...
January 21, 2017: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/28110389/a-novel-lectin-from-artocarpus-lingnanensis-induces-proliferation-and-th1-th2-cytokine-secretion-through-cd45-signaling-pathway-in-human-t-lymphocytes
#3
Bo Cui, Lu Li, Qiyan Zeng, Faquan Lin, Lijun Yin, Liejun Liao, Min Huang, Jingping Wang
Lectins are carbohydrate-binding proteins and have been used for purification and characterization of glycoproteins. In this study, a novel 58.9-kDa tetrameric lectin from Artocarpus lingnanensis seeds was purified, characterized, and its mitogenic potential was evaluated. The hemagglutination inhibition assay indicated that Artocarpus lingnanensis lectin (ALL) showed specificity toward galactose. ALL was effectively purified in a single-step using affinity chromatography on a galactose-Sepharose column. ALL showed pH optima between 5...
January 21, 2017: Journal of Natural Medicines
https://www.readbyqxmd.com/read/28110368/comparison-of-the-local-effects-of-a-600-%C3%AE-m-bare-fibre-at-high-laser-power-on-lung-parenchyma-nd-yag-laser-1320-vs-1064%C3%A2-nm
#4
Andreas Kirschbaum, Detlef K Bartsch, Peter Rexin
Lung metastases are often resected non-anatomically with a laser using a diode-pumped Nd:YAG laser at a wavelength of 1320 nm with a laser output of up to 60 W. Usually the removal of lesions is carried out in contact mode by means of a bare fibre. We compared the local effects of an Nd:YAG laser at a wavelength of 1064 nm with those at a wavelength of 1320 nm using a 600-μm bare fibre in contact mode in an experimental model. The investigations were carried out on porcine lungs freshly withdrawn at the abattoir...
January 21, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28110261/structure-and-chemistry-of-epitaxial-ceria-thin-films-on-yttria-stabilized-zirconia-substrates-studied-by-high-resolution-electron-microscopy
#5
Robert Sinclair, Sang Chul Lee, Yezhou Shi, William C Chueh
We have applied aberration-corrected transmission electron microscopy (TEM) imaging and electron energy loss spectroscopy (EELS) to study the structure and chemistry of epitaxial ceria thin films, grown by pulsed laser deposition onto (001) yttria-stabilized zirconia (YSZ) substrates. There are few observable defects apart from the expected mismatch interfacial dislocations and so the films would be expected to have good potential for applications. Under high electron beam dose rate (above about 6000 e(-)/Å(2)s) domains of an ordered structure appear and these are interpreted as being created by oxygen vacancy ordering...
January 6, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28110178/tem-sample-preparation-by-femtosecond-laser-machining-and-ion-milling-for-high-rate-tem-straining-experiments
#6
Thomas Voisin, Michael D Grapes, Yong Zhang, Nicholas Lorenzo, Jonathan Ligda, Brian Schuster, Timothy P Weihs
To model mechanical properties of metals at high strain rates, it is important to visualize and understand their deformation at the nanoscale. Unlike post mortem Transmission Electron Microscopy (TEM), which allows one to analyze defects within samples before or after deformation, in situ TEM is a powerful tool that enables imaging and recording of deformation and the associated defect motion during mechanical loading. Unfortunately, all current in situ TEM mechanical testing techniques are limited to quasi-static strain rates...
December 5, 2016: Ultramicroscopy
https://www.readbyqxmd.com/read/28110070/mesoscale-porosity-at-the-dentin-enamel-junction-could-affect-the-biomechanical-properties-of-teeth
#7
Elsa Vennat, Wenlong Wang, Rachel Genthial, Bertrand David, Elisabeth Dursun, Aurélien Gourrier
: In this paper, the 3D-morphology of the porosity in dentin is investigated within the first 350 μm from the dentin-enamel junction (DEJ) by fluorescence confocal laser scanning microscopy (CLSM). We found that the porous microstructure exhibits a much more complex geometry than classically described, which may impact our fundamental understanding of the mechanical behavior of teeth and could have practical consequences for dental surgery. Our 3D observations reveal numerous fine branches stemming from the tubules which may play a role in cellular communication or mechanosensing during the early stages of dentinogenesis...
January 18, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28109550/rapid-detection-of-high-risk-enterococcus-faecium-clones-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#8
Ana R Freitas, Clara Sousa, Carla Novais, Liliana Silva, Helena Ramos, Teresa M Coque, João Lopes, Luísa Peixe
We aimed to explore the potential of matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for early identification of dominant Enterococcus faecium (Efm) clones involved in human infections. Well-characterized Efm isolates (n=77), analyzed by pulsed-field gel electrophoresis and multilocus sequence typing(eBURST and BAPS [Bayesian analysis of population structure] algorithms), and belonging to different hospital (n=53) and community (n=24) phylogenomic groups, were tested...
December 14, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28109521/giant-periurethral-condyloma-acuminata-in-a-2-year-old-girl-successful-treatment-with-carbon-dioxide-laser
#9
X Calderón-Castrat, S Blanco, J C Santos-Durán, M Roncero-Riesco
No abstract text is available yet for this article.
January 18, 2017: Actas Dermo-sifiliográficas
https://www.readbyqxmd.com/read/28109295/a-randomised-active-and-placebo-controlled-three-period-crossover-trial-to-investigate-short-term-effects-of-the-dipeptidyl-peptidase-4-inhibitor-linagliptin-on-macro-and-microvascular-endothelial-function-in-type-2-diabetes
#10
Thomas Jax, Alin Stirban, Arne Terjung, Habib Esmaeili, Andreas Berk, Sandra Thiemann, Robert Chilton, Maximilian von Eynatten, Nikolaus Marx
BACKGROUND: Studies of dipeptidyl peptidase (DPP)-4 inhibitors report heterogeneous effects on endothelial function in patients with type 2 diabetes (T2D). This study assessed the effects of the DPP-4 inhibitor linagliptin versus the sulphonylurea glimepiride and placebo on measures of macro- and microvascular endothelial function in patients with T2D who represented a primary cardiovascular disease prevention population. METHODS: This crossover study randomised T2D patients (n = 42) with glycated haemoglobin (HbA1c) ≤7...
January 21, 2017: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/28109170/comparative-proteomic-analysis-of-urine-and-laser-microdissected-glomeruli-in-iga-nephropathy
#11
Xiaoyuan Ning, Zhong Yin, Zuoxiang Li, Jiayun Xu, Linna Wang, Wanjun Shen, Yang Lu, Guangyan Cai, Xueguang Zhang, Xiangmei Chen
The purpose of the present study was to identify the differential proteins that synchronously change in urine and glomeruli and could be used to monitor glomerular lesions of IgA nephropathy (IgAN). The proteomes of urine and glomeruli from 4 IgAN patients who were graded III/IV according to the grading system of Lee et al were compared to the urine proteomes of 4 healthy volunteers and the glomeruli proteomes of adjacent normal tissue from 4 patients with renal tumors, respectively. Western blot, enzyme-linked immunosorbent assay and immunofluorescence assay were applied to verify the results of the proteomic analysis...
January 21, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28109117/lipid-profiling-of-parkin-mutant-human-skin-fibroblasts
#12
Simona Lobasso, Paola Tanzarella, Daniele Vergara, Michele Maffia, Tiziana Cocco, Angela Corcelli
Parkin mutations are a major cause of early-onset Parkinson's disease (PD). The impairment of protein quality control system together with defects in mitochondria and autophagy process are consequences of the lack of parkin, which leads to neurodegeneration. Little is known about the role of lipids in these alterations of cell functions. In the present study parkin-mutant human skin primary fibroblasts have been considered as cellular model of PD to investigate on possible lipid alterations associated with the lack of parkin protein...
January 21, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28108904/intravitreal-anti-vegf-treatment-for-subretinal-neovascularisation-secondary-to-type-2-idiopathic-juxtafoveolar-telangiectasia
#13
Teresa Barth, Florian Zeman, Horst Helbig, Maria-Andreea Gamulescu
PURPOSE: To assess the long-term outcome of patients with subretinal neovascular membrane (SRNVM) secondary to type 2 idiopathic juxtafoveolar telangiectasia (IJT) receiving intravitreal anti-VEGF (vascular endothelial growth factor) injections. METHODS: A total of 14 eyes of 12 patients treated with intravitreal anti-VEGF for SRNVM related to type 2 IJT were retrospectively assessed. RESULTS: Nine men and 3 women with a mean age of 66 years (SD 12, range 47-87 years) were diagnosed with IJT-related SRNVM...
January 21, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28108825/photodynamic-therapy-for-the-treatment-of-complex-anal-fistula
#14
A Arroyo, P Moya, M A Rodríguez-Prieto, M J Alcaide, M M Aguilar, M Bellón, M T Pérez-Vázquez, F Candela, R Calpena
BACKGROUND: Photodynamic therapy (PDT) is a new procedure for the treatment of anal fistula. This preliminary study was designed to investigate the safety and effectiveness of this new technique in the treatment of anal fistula. METHODS: Ten patients were treated with PDT. Intralesional 5-aminolevulinic acid (ALA) 2% was directly injected into the fistula. The internal and external orifices were closed. After an incubation period of 2 h, the fistula was irradiated using an optical fibre connected to a red laser (MULTIDIODE 630 PDT, INTERmedic, Spain) operating at 1 W/cm for 3 min (180 Joules)...
January 20, 2017: Techniques in Coloproctology
https://www.readbyqxmd.com/read/28108568/the-role-of-day-one-postoperative-review-of-intraocular-pressure-in-modern-vitrectomy-surgery
#15
Nicholas Brennan, Ian Reekie, Eric Ezra, Keith Barton, Ananth Viswanathan, Mahiul Mk Muqit
BACKGROUND: With the introduction of sutureless 23-gauge (23G) vitrectomy, the risks for elevated intraocular pressure (IOP) and the need for day 1 review of IOP are less certain. AIMS: To assess current practice for postoperative review in the vitreoretinal service at a large tertiary referral centre; to assess whether day 1 review detected complications altering patient management; to identify risk factors for IOP spikes postvitrectomy. METHODS: Retrospective, consecutive case note review of all patients undergoing 23G vitrectomy at the study institution between April and June 2013...
January 20, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28108378/the-pressure-induced-deformation-response-of-the-human-lamina-cribrosa-analysis-of-regional-variations
#16
Dan E Midgett, Mary E Pease, Joan L Jefferys, Mohak Patel, Christian Franck, Harry A Quigley, Thao D Nguyen
: The objective of this study was to measure the pressure-induced deformation response of the human lamina cribrosa (LC) and analyze for variations with age and anatomical region. The posterior scleral cup of 8 eyes from 6 human donors was mounted onto a custom inflation chamber. A laser-scanning microscope was used for second harmonic generation (SHG), imaging the collagen structure in the posterior volume of the LC at pressures from 5 mmHg to 45 mmHg. The SHG volumes were analyzed by the Fast-Fourier Iterative Digital Volume Correlation (DVC) algorithm for the three dimensional (3D) displacement field...
January 17, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28108308/role-of-cyclooxygenase-in-the-vascular-response-to-locally-delivered-acetylcholine-in-caucasian-and-african-descent-individuals
#17
Matthew J Maley, James R House, Michael J Tipton, Clare M Eglin
INTRODUCTION: Individuals of African descent (AFD) are more susceptible to non-freezing cold injury (NFCI) compared with Caucasian individuals (CAU). Vasodilatation to acetylcholine (ACh) is lower in AFD compared with CAU in the non-glabrous foot and finger skin sites; the reason for this is unknown. Prostanoids are responsible, in part, for the vasodilator response to ACh, however it is not known whether the contribution differs between ethnicities. METHODS: 12 CAU and 12 AFD males received iontophoresis of ACh (1 w/v%) on non-glabrous foot and finger skin sites following placebo and then aspirin (600mg, single blinded)...
January 17, 2017: Microvascular Research
https://www.readbyqxmd.com/read/28107941/mil-101-cr-as-matrix-for-sensitive-detection-of-quercetin-by-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#18
Guobin Han, Qiaoling Zeng, Zhongwei Jiang, Tiantian Xing, Chengzhi Huang, Yuanfang Li
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been proven as a useful and advanced technique in the identification of polymers and proteins. However, MALDI-TOF-MS still has the unavoidable drawback of self-signal interference with traditional organic matrices, which could suppress and overlap with the analyte signals in the low-mass region. In this work, MIL-101(Cr), a kind of metal-organic frameworks which possess high molecular weight, π-conjugated 3-D structure, coordinately unsaturated chromium sites (CUS) and strong absorption in the UV range, was employed to replace traditional organic matrices, and it was found that MIL-101(Cr) can dramatically eliminate the background peaks, showing high signal-to-noise level in the analysis of small molecules...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107937/calibration-free-quantitative-analysis-of-elemental-ratios-in-intermetallic-nanoalloys-and-nanocomposites-using-laser-induced-breakdown-spectroscopy-libs
#19
Seyyed Ali Davari, Sheng Hu, Dibyendu Mukherjee
Intermetallic nanoalloys (NAs) and nanocomposites (NCs) have increasingly gained prominence as efficient catalytic materials in electrochemical energy conversion and storage systems. But their morphology and chemical compositions play critical role in tuning their catalytic activities, and precious metal contents. While advanced microscopy techniques facilitate morphological characterizations, traditional chemical characterizations are either qualitative or extremely involved. In this study, we apply Laser Induced Breakdown Spectroscopy (LIBS) for quantitative compositional analysis of NAs and NCs synthesized with varied elemental ratios by our in-house built pulsed laser ablation technique...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107912/maldi-ltq-orbitrap-mass-spectrometry-imaging-for-lipidomic-analysis-in-kidney-under-cisplatin-chemotherapy
#20
Estefanía Moreno-Gordaliza, Diego Esteban-Fernández, Alberto Lázaro, Blanca Humanes, Sarah Aboulmagd, Alberto Tejedor, Michael W Linscheid, M Milagros Gómez-Gómez
Imaging techniques for mapping molecular distributions in tissue sections can reveal valuable information on biomolecules involved in relevant biochemical processes. A method has been developed for comprehensive, reproducible and sensitive lipid imaging by matrix-assisted laser/desorption ionization-LTQ-Orbitrap mass spectrometry in kidney sections, showing the benefits of exact mass determination. Matrix deposition parameters for positive and negative lipid ion imaging using different matrices such as 2,5-dihydroxybenzoic acid (DHB), 9-aminoacridine (9-AA) or α-cyano-4-hydroxycinnamic acid (CHCA) have been optimized for the broadest detection and identification of renal lipids...
March 1, 2017: Talanta
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