keyword
MENU ▼
Read by QxMD icon Read
search

LADA

keyword
https://www.readbyqxmd.com/read/28088294/suitability-of-small-diagnostic-peripheral-blood-samples-for-cell-therapy-studies
#1
Coralea Stephanou, Panayiota Papasavva, Myria Zachariou, Petros Patsali, Marilena Epitropou, Petros Ladas, Ruba Al-Abdulla, Soteroulla Christou, Michael N Antoniou, Carsten W Lederer, Marina Kleanthous
BACKGROUND AIMS: Primary hematopoietic stem and progenitor cells (HSPCs) are key components of cell-based therapies for blood disorders and are thus the authentic substrate for related research. We propose that ubiquitous small-volume diagnostic samples represent a readily available and as yet untapped resource of primary patient-derived cells for cell- and gene-therapy studies. METHODS: In the present study we compare isolation and storage methods for HSPCs from normal and thalassemic small-volume blood samples, considering genotype, density-gradient versus lysis-based cell isolation and cryostorage media with different serum contents...
February 2017: Cytotherapy
https://www.readbyqxmd.com/read/28065264/a-new-method-to-determine-the-transmembrane-conformation-of-substrates-in-intramembrane-proteolysis-by-deep-uv-resonance-raman-spectroscopy
#2
J W Cooley, A Abdine, M Brown, J Chavez, B Lada, R D JiJi, I Ubarretxena-Belandia
We present a new method based on deep-UV resonance Raman spectroscopy to determine the backbone conformation of intramembrane protease substrates. The classical amide vibrational modes reporting on the conformation of just the transmembrane region of the substrate can be resolved from solvent exchangeable regions outside the detergent micelle by partial deuteration of the solvent. In the presence of isotopically triple-labeled intramembrane protease, these amide modes can be accurately measured to monitor the transmembrane conformation of the substrate during intramembrane proteolysis...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28052296/clinical-and-biochemical-influence-of-prostatic-stones
#3
Tomislav Soric, Mirnes Selimovic, Lada Bakovic, Tatjana Šimurina, Robert Selthofer, Jerka Dumic
INTRODUCTION: The study aimed to explore clinical influence of prostatic stones on lower urinary tract symptoms (LUTS), seminal plasma cytokines, and serum biomarkers. MATERIALS AND METHODS: A total of 70 men aged ≤50 years with LUTS divided into 2 groups: group with stones (GSt) and group without prostatic stones (GNoSt). All subjects completed the International Prostate Symptom Score (IPSS) questionnaire and National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI) scoring questionnaire...
January 5, 2017: Urologia Internationalis
https://www.readbyqxmd.com/read/28051281/genetic-variation-in-leptin-and-leptin-receptor-genes-is-a-risk-factor-for-idiopathic-recurrent-spontaneous-abortion
#4
Andrijana Müller, Jasenka Wagner, Alenka Hodžić, Aleš Maver, Ivana Škrlec, Marija Heffer, Lada Zibar, Borut Peterlin
AIM: To determine whether maternal leptin (LEP) and leptin receptor (LEPR) gene polymorphisms are associated with idiopathic recurrent spontaneous abortion (IRSA). METHODS: This case-control association study conducted from 2010 to 2012 at the Department of Gynecology and Obstetrics, University Hospital Center Osijek and Clinical Institute of Medical Genetics Ljubljana included 178 women with a history of three or more IRSAs before the 22nd week of gestation and 145 women with at least two live births and no history of pathologic pregnancies during reproductive period...
December 31, 2016: Croatian Medical Journal
https://www.readbyqxmd.com/read/28011361/unexpected-effect-of-dry-olive-leaf-extract-on-the-level-of-dna-damage-in-lymphocytes-of-lead-intoxicated-workers-before-and-after-cana2edta-chelation-therapy
#5
Andrea Čabarkapa, Dragana Dekanski, Lada Živković, Mirjana Milanović-Čabarkapa, Vladan Bajić, Dijana Topalović, Francesca Giampieri, Massimiliano Gasparrini, Maurizio Battino, Biljana Spremo-Potparević
The CaNa2EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa2EDTA chelation therapy. DOLE demonstrated pronounced radical scavenging activity in concentrations ≥1 mg/mL, and showed no genotoxicity per se, in concentrations 0...
December 21, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28005367/size-dependent-structure-relations-between-nanotubes-and-encapsulated-nanocrystals
#6
Andrei A Eliseev, Nikolay S Falaleev, Nikolay I Verbitskiy, Andrei A Volykhov, Lada V Yashina, Andrei S Kumskov, Victoria G Zhigalina, Alexander L Vasiliev, Alexey V Lukashin, Jeremy Sloan, Nikolay A Kiselev
The structural organization of compounds in a confined space of nanometer-scale cavities is of fundamental importance for understanding the basic principles for atomic structure design at the nanolevel. Here, we explore size-dependent structure relations between one-dimensional PbTe nanocrystals and carbon nanotube containers in the diameter range of 2.0-1.25 nm using high-resolution transmission electron microscopy and ab initio calculations. Upon decrease of the confining volume, one-dimensional crystals reveal gradual thinning, with the structure being cut from the bulk in either a <110> or a <100> growth direction until a certain limit of ∼1...
January 18, 2017: Nano Letters
https://www.readbyqxmd.com/read/27984548/capillary-nonperfusion-and-photoreceptor-loss-in-branch-retinal-vein-occlusion-spatial-correlation-and-morphologic-characteristics
#7
Menelaos G Kanakis, Konstantina Giannouli, Konstantinos Andreanos, Dimitrios Papaconstantinou, Chrysanthi Koutsandrea, Ioannis Ladas, Ilias Georgalas, Petros Petrou, Athanasios I Kotsolis
PURPOSE: To evaluate the photoreceptor layer in eyes with branch retinal vein occlusion associated with macular ischemia, using a method of en face optical coherence tomography (OCT) representation of the ellipsoid zone. METHODS: Customized macular OCT scans of 9 patients (10 eyes) with branch retinal vein occlusion and macular ischemia were exported and subsequently postprocessed (removal of vascular and cystic spaces' shadows, segmentation, and alignment to the retinal pigment epithelium)...
December 14, 2016: Retina
https://www.readbyqxmd.com/read/27979625/a-method-to-identify-drivers-of-societal-change-likely-to-affect-natural-assets-in-the-future-illustrated-with-australia-s-native-biodiversity
#8
D A Pepper, Hania Lada, James R Thomson, K Shuvo Bakar, P S Lake, Ralph Mac Nally
Human society has a profound adverse effect on natural assets as human populations increase and as global climate changes. We need to envisage different futures that encompass plausible human responses to threats and change, and become more mindful of their likely impacts on natural assets. We describe a method for developing a set of future scenarios for a natural asset at national scale under ongoing human population growth and climate change. The method involves expansive consideration of potential drivers of societal change, a reduction of these to form a small set of key drivers to which contrasting settings are assigned, which we use to develop a set of different scenarios...
December 13, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27934986/a-unique-copper-ii-assisted-transformation-of-acetylacetone-dioxime-in-acetone-that-leads-to-one-dimensional-quinoxaline-bridged-coordination-polymers
#9
Zoi G Lada, Amaia Soto Beobide, Aikaterini Savvidou, Catherine P Raptopoulou, Vassilis Psycharis, George A Voyiatzis, Mark M Turnbull, Spyros P Perlepes
The reactions of copper(ii) carboxylate sources with acetylacetone dioxime (acacdoH2) in Me2CO have been studied and a novel, metal ion-assisted ligand transformation has been discovered. The reaction of [Cu2(diba)4(dibaH)2] and acacdoH2 (1 : 1.5) in Me2CO has provided access to the complex {[Cu2(diba)4(qunx)]}n (1) in low yield (25-30%), where dibaH is 3,3-dimethylbutyric acid and qunx is quinoxaline. The [Cu2(piv)4(pivH)2]/acacdoH2 (1 : 1.5) reaction system in warm Me2CO, where pivH is pivalic acid, gave the analogous complex {[Cu2(piv)4(qunx)]}n (2) in moderate yield (∼50%)...
December 20, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27931088/sglt2-inhibitors-a-systematic-review-of-diabetic-ketoacidosis-and-related-risk-factors-in-the-primary-literature
#10
Kelly R Burke, Christine A Schumacher, Spencer E Harpe
STUDY OBJECTIVE: There is currently minimal information regarding risk factors for the development of sodium-glucose cotransporter-2 inhibitor (SGLT2i)-related diabetic ketoacidosis (DKA). We aim to identify individual patient characteristics associated with cases of SGLT2i-related DKA to better describe potential risk factors. DESIGN: Systematic review of primary literature. PATIENTS: 34 case reports of patients with Type 1 and Type 2 Diabetes Mellitus who developed DKA while receiving an SGLT2i...
December 8, 2016: Pharmacotherapy
https://www.readbyqxmd.com/read/27926472/sweetened-beverage-intake-and-risk-of-latent-autoimmune-diabetes-in-adults-lada-and-type-2-diabetes
#11
Josefin E Löfvenborg, Tomas Andersson, Per-Ola Carlsson, Mozhgan Dorkhan, Leif Groop, Mats Martinell, Tiinamaija Tuomi, Alicja Wolk, Sofia Carlsson
OBJECTIVE: Sweetened beverage intake is associated with increased risk of type 2 diabetes, but its association with autoimmune diabetes is unclear. We aimed to investigate sweetened beverage intake and risk of latent autoimmune diabetes in adults (LADA); autoimmune diabetes with features of type 2 diabetes. DESIGN/METHODS: Data from a Swedish population-based study was used, including incident cases of LADA (n = 357) and type 2 diabetes (n = 1136) and randomly selected controls (n = 1371)...
December 2016: European Journal of Endocrinology
https://www.readbyqxmd.com/read/27924834/activation-induced-deaminase-mutational-signature-overlaps-with-cpg-methylation-sites-in-follicular-lymphoma-and-other-cancers
#12
Igor B Rogozin, Artem G Lada, Alexander Goncearenco, Michael R Green, Subhajyoti De, German Nudelman, Anna R Panchenko, Eugene V Koonin, Youri I Pavlov
Follicular lymphoma (FL) is an uncurable cancer characterized by progressive severity of relapses. We analyzed sequence context specificity of mutations in the B cells from a large cohort of FL patients. We revealed substantial excess of mutations within a novel hybrid nucleotide motif: the signature of somatic hypermutation (SHM) enzyme, Activation Induced Deaminase (AID), which overlaps the CpG methylation site. This finding implies that in FL the SHM machinery acts at genomic sites containing methylated cytosine...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27913577/metabolite-profiling-of-lada-challenges-the-view-of-a-metabolically-distinct-subtype
#13
Mahmoud Al-Majdoub, Arslan Ali, Petter Storm, Anders Rosengren, Leif Groop, Peter Spégel
Latent autoimmune diabetes in adults (LADA) usually refers to GADAb positive diabetes with onset after 35 years of age and no insulin treatment within the first 6 months after diagnosis. However, it is not always easy to distinguish LADA from type 1 or type 2 diabetes.In this study we examined whether metabolite profiling could help to distinguish LADA (N=50) from type 1 (N=50) and type 2 diabetes (N=50).Out of 123 identified metabolites, 99 differed between the diabetes types. However, no unique metabolite profile could be identified for any of the types...
December 2, 2016: Diabetes
https://www.readbyqxmd.com/read/27903892/methylation-sensitive-enrichment-of-minor-dna-alleles-using-a-double-strand-dna-specific-nuclease
#14
Yibin Liu, Chen Song, Ioannis Ladas, Mariana Fitarelli-Kiehl, G Mike Makrigiorgos
Aberrant methylation changes, often present in a minor allelic fraction in clinical samples such as plasma-circulating DNA (cfDNA), are potentially powerful prognostic and predictive biomarkers in human disease including cancer. We report on a novel, highly-multiplexed approach to facilitate analysis of clinically useful methylation changes in minor DNA populations. Methylation Specific Nuclease-assisted Minor-allele Enrichment (MS-NaME) employs a double-strand-specific DNA nuclease (DSN) to remove excess DNA with normal methylation patterns...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27899085/oestradiol-levels-and-superoxide-dismutase-activity-in-age-related-cataract-a-case-control-study
#15
Dragana Škiljić, Staffan Nilsson, Anne Petersen, Jan-Olof Karlsson, Anders Behndig, Lada Kalaboukhova, Madeleine Zetterberg
BACKGROUND: It has been suggested that the higher prevalence of cataract in women is caused by a withdrawal effect of oestrogen at menopause. In vitro studies have demonstrated protection of serum oestradiol (E2) against oxidative stress through upregulation of antioxidant enzymes, including superoxide dismutase (SOD). The purpose of the present study was to investigate E2 levels and SOD erythrocyte activity in patients with age-related cataract. METHODS: The studied subjects consisted of 103 patients with age-related cataract and 22 controls...
November 29, 2016: BMC Ophthalmology
https://www.readbyqxmd.com/read/27891056/eradication-of-helicobacter-pylori-infection-restores-ki67-p53-and-cyclin-d1-immunoreactivity-in-the-human-gastric-epithelium
#16
Konstantinos Triantafyllou, Vasilios Papadopoulos, Theodoros Emanouil, Paraskevas Gkolfakis, Vasileia Damaskou, Georgios Tziatzios, Ioannis G Panayiotides, Irene Vafiadis, Spiros D Ladas
INTRODUCTION: We evaluated the effect of Helicobacter pylori (HP) eradication on p53, cyclin D1 expression, and cell proliferation in gastric mucosa. MATERIALS AND METHODS: We assessed p53, cyclin D1, and ki67 immunoexpression in gastric mucosa from 31 HP chronic gastritis patients and 12 controls. Reassessment was performed 6 months after successful HP eradication. RESULTS: Successful eradication resulted in significant decrease of p53 (1...
2016: Clinical Medicine Insights. Gastroenterology
https://www.readbyqxmd.com/read/27873357/use-of-big-data-for-drug-development-and-for-public-and-personal-health-and-care
#17
Lada Leyens, Matthias Reumann, Nuria Malats, Angela Brand
The use of data analytics across the entire healthcare value chain, from drug discovery and development through epidemiology to informed clinical decision for patients or policy making for public health, has seen an explosion in the recent years. The increase in quantity and variety of data available together with the improvement of storing capabilities and analytical tools offer numerous possibilities to all stakeholders (manufacturers, regulators, payers, healthcare providers, decision makers, researchers) but most importantly, it has the potential to improve general health outcomes if we learn how to exploit it in the right way...
January 2017: Genetic Epidemiology
https://www.readbyqxmd.com/read/27866814/predictors-of-tissue-healing-in-ulcerative-colitis-patients-treated-with-anti-tnf
#18
Nikos Viazis, Marios Giakoumis, Giorgos Bamias, Dimitris Goukos, Theodoros Koukouratos, Konstantina Katopodi, Pantelis Karatzas, Christos Triantos, Chrisostomos Tsolias, Georgios Theocharis, George L Daikos, Spyros D Ladas, Dimitrios G Karamanolis, Gerasimos J Mantzaris
AIM: To identify factors predicting mucosal healing in ulcerative colitis patients treated with anti-TNFα agents with or without azathioprine. METHODS: In a prospective, multicenter, one-year study biologic naïve patients aged 25-65 years, with corticosteroid-dependent or refractory colitis received combination treatment with anti-TNFα and azathioprine for 6 months followed by anti-TNFα monotherapy. Patients who denied combination therapy or were outside this age range received anti-TNFα monotherapy (controls)...
January 2017: Digestive and Liver Disease
https://www.readbyqxmd.com/read/27856268/validation-of-the-new-sepsis-3-definitions-proposal-for-improvement-in-early-risk-identification
#19
E J Giamarellos-Bourboulis, T Tsaganos, I Tsangaris, M Lada, C Routsi, D Sinapidis, M Koupetori, M Bristianou, G Adamis, K Mandragos, G N Dalekos, I Kritselis, G Giannikopoulos, I Koutelidakis, M Pavlaki, E Antoniadou, G Vlachogiannis, V Koulouras, A Prekates, G Dimopoulos, A Koutsoukou, I Pnevmatikos, A Ioakeimidou, A Kotanidou, S E Orfanos, A Armaganidis, C Gogos
OBJECTIVES: Sepsis-3 definitions generated controversies regarding their general applicability. The Sepsis-3 Task Force outlined the need for validation with emphasis on the quick Sequential Organ Failure Assessment (qSOFA) score. This was done in a prospective cohort from a different healthcare setting. METHODS: Patients with infections and at least two signs of systemic inflammatory response syndrome (SIRS) were analysed. Sepsis was defined as total SOFA ≥2 outside the intensive care unit (ICU) or as an increase of ICU admission SOFA ≥2...
November 14, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27853030/the-prevalence-and-characteristics-of-latent-autoimmune-diabetes-in-adults-subset-among-type-two-diabetes-mellitus-patients-in-northern-nigeria
#20
Salisu Babura Muazu, Innocent Okpe, Felicia Anumah
INTRODUCTION: Latent autoimmune diabetes in adult (LADA) is a form of Type 1 diabetes mellitus (T1DM) that occurs in adult or with advancing age. It commonly occurs in people aged ≥30 years and is characterized by initial response to oral hypoglycemic agents, lean body mass, and presence of glutamic acid decarboxylase autoantibody (GAD-Ab). It exhibits rapid deterioration of the pancreatic β-cells secretory function due to the destructive action of the autoantibodies. The prevalence of LADA among T2DM patients varies among population due to different diagnostic criteria, patients' characteristics, the assay used, and genetic predisposition...
October 2016: Annals of African Medicine
keyword
keyword
23263
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"