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https://www.readbyqxmd.com/read/29454291/de-novo-transcriptome-of-the-pallial-gland-of-the-date-mussel-lithophaga-lithophaga
#1
Urška Sivka, Nataša Toplak, Simon Koren, Jernej Jakše
Lithophaga lithophaga is a rock-boring bivalve with an ability to dissolve carbonate substrata with its siphonal and pallial gland secretions. However, the molecular mechanism that enables this species to bore into calcareous rocks is not yet known. In order to identify genes potentially involved in chemical boring we performed transcriptome sequencing of pallial-gland tissue samples of L. lithophaga. Transcriptome sequencing using an Ion Torrent platform generated 60.563 million clean reads with an average read length of 96 bp...
February 6, 2018: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/29454219/reading-skill-related-to-left-ventral-occipitotemporal-cortex-during-a-phonological-awareness-task-in-5-6-year-old-children
#2
Jin Wang, Marc F Joanisse, James R Booth
The left ventral occipitotemporal cortex (vOT) is important in visual word recognition. Studies have shown that the left vOT is generally observed to be involved in spoken language processing in skilled readers, suggesting automatic access to corresponding orthographic information. However, little is known about where and how the left vOT is involved in the spoken language processing of young children with emerging reading ability. In order to answer this question, we examined the relation of reading ability in 5-6-year-old kindergarteners to the activation of vOT during an auditory phonological awareness task...
February 14, 2018: Developmental Cognitive Neuroscience
https://www.readbyqxmd.com/read/29454215/effect-based-trigger-values-for-in-vitro-and-in-vivo-bioassays-performed-on-surface-water-extracts-supporting-the-environmental-quality-standards-eqs-of-the-european-water-framework-directive
#3
Beate I Escher, Selim Aїt-Aїssa, Peter A Behnisch, Werner Brack, François Brion, Abraham Brouwer, Sebastian Buchinger, Sarah E Crawford, David Du Pasquier, Timo Hamers, Karina Hettwer, Klára Hilscherová, Henner Hollert, Robert Kase, Cornelia Kienle, Andrew J Tindall, Jochen Tuerk, Ron van der Oost, Etienne Vermeirssen, Peta A Neale
Effect-based methods including cell-based bioassays, reporter gene assays and whole-organism assays have been applied for decades in water quality monitoring and testing of enriched solid-phase extracts. There is no common EU-wide agreement on what level of bioassay response in water extracts is acceptable. At present, bioassay results are only benchmarked against each other but not against a consented measure of chemical water quality. The EU environmental quality standards (EQS) differentiate between acceptable and unacceptable surface water concentrations for individual chemicals but cannot capture the thousands of chemicals in water and their biological action as mixtures...
February 14, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29454113/identification-of-two-novel-cress-dna-viruses-associated-with-an-avipoxvirus-lesion-of-a-blue-and-gray-tanager-thraupis-episcopus
#4
Michaël A J Moens, Javier Pérez-Tris, Martí Cortey, Laura Benítez
The discovery of circular rep-encoding single stranded (CRESS) DNA viruses has increased spectacularly over the past decade. They represent the smallest animal viruses known worldwide infecting a wide variety of invertebrates and vertebrates in different natural and human-made environments. The extremely low similarity of nucleotide and protein sequences among different CRESS DNA genomes has challenged their classification. Moreover, the existence of capsid proteins (Cp) remains difficult to demonstrate which is crucial to understand the structural properties of these viruses...
February 14, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29453922/a-short-review-of-primary-aldosteronism-in-a-question-and-answer-fashion
#5
Frederick-Anthony Farrugia, Nicolaos Zavras, Georgios Martikos, Panagiotis Tzanetis, Anestis Charalampopoulos, Evangelos P Misiakos, Dimitrios Sotiropoulos, Nikolaos Koliakos
OBJECTIVES: The aim of this study was to present up to date information concerning the diagnosis and treatment of primary aldosteronism (PA). PA is the most common cause of endocrine hypertension. It has been reported up to 24% of selective referred hypertensive patients. METHODS: We did a search in Pub-Med and Google Scholar using the terms: PA, hyperaldosteronism, idiopathic adrenal hyperplasia, diagnosis of PA, mineralocorticoid receptor antagonists, adrenalectomy, and surgery...
January 1, 2018: Endocrine Regulations
https://www.readbyqxmd.com/read/29453633/comparative-analysis-of-the-mitochondrial-genome-of-the-fungus-colletotrichum-lindemuthianum-the-causal-agent-of-anthracnose-in-common-beans
#6
Casley Borges de Queiroz, Mateus Ferreira Santana, Pedro M Pereira Vidigal, Marisa Vieira de Queiroz
Fungi of the genus Colletotrichum are economically important and are used as models in plant-pathogen interaction studies. In this study, the complete mitochondrial genomes of two Colletotrichum lindemuthianum isolates were sequenced and compared with the mitochondrial genomes of seven species of Colletotrichum. The mitochondrial genome of C. lindemuthianum is a typical circular molecule 37,446 bp (isolate 89 A 2 2-3) and 37,440 bp (isolate 83.501) in length. The difference of six nucleotides between the two genomes is the result of a deletion in the ribosomal protein S3 (rps3) gene in the 83...
February 16, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29453477/effects-of-bilateral-nasal-packing-on-oxygen-saturation-and-blood-pressure
#7
Meera Bista
INTRODUCTION: Nasal packing is one of the most common rhinological procedures in ENT practice exerting local in nose and systemic effects. Systemic effects may be due to poor sleep quality, respiratory difficulty, decreased oxygen saturation, circulatory problems and toxic shock syndrome which can threaten the life of a person.The study was done to investigate the effect on bilateral nasal packing on blood oxygen saturation and blood pressure changes. METHODS: A cross-sectional prospective study was undertaken in Kathmandu Medical College from January 2017 to June 2017...
October 2017: JNMA; Journal of the Nepal Medical Association
https://www.readbyqxmd.com/read/29453452/systematic-and-stochastic-influences-on-the-performance-of-the-minion-nanopore-sequencer-across-a-range-of-nucleotide-bias
#8
Raga Krishnakumar, Anupama Sinha, Sara W Bird, Harikrishnan Jayamohan, Harrison S Edwards, Joseph S Schoeniger, Kamlesh D Patel, Steven S Branda, Michael S Bartsch
Emerging sequencing technologies are allowing us to characterize environmental, clinical and laboratory samples with increasing speed and detail, including real-time analysis and interpretation of data. One example of this is being able to rapidly and accurately detect a wide range of pathogenic organisms, both in the clinic and the field. Genomes can have radically different GC content however, such that accurate sequence analysis can be challenging depending upon the technology used. Here, we have characterized the performance of the Oxford MinION nanopore sequencer for detection and evaluation of organisms with a range of genomic nucleotide bias...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453430/increased-deficit-of-visual-attention-span-with-development-in-chinese-children-with-developmental-dyslexia
#9
Jing Zhao, Menglian Liu, Hanlong Liu, Chen Huang
It has been suggested that orthographic transparency and age changes may affect the relationship between visual attention span (VAS) deficit and reading difficulty. The present study explored the developmental trend of VAS in children with developmental dyslexia (DD) in Chinese, a logographic language with a deep orthography. Fifty-seven Chinese children with DD and fifty-four age-matched normal readers participated. The visual 1-back task was adopted to examine VAS. Phonological and morphological awareness tests, and reading tests in single-character and sentence levels were used for reading skill measurements...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453368/effects-of-timber-harvesting-on-the-genetic-potential-for-carbon-and-nitrogen-cycling-in-five-north-american-forest-ecozones
#10
Erick Cardenas, Luis H Orellana, Konstantinos T Konstantinidis, William W Mohn
Forest ecosystems are critical to global biogeochemical cycles but under pressure from harvesting and climate change. We investigated the effects of organic matter (OM) removal during forest harvesting on the genetic potential of soil communities for biomass decomposition and nitrogen cycling in five ecozones across North America. We analyzed 107 samples, representing four treatments with varied levels of OM removal, at Long-Term Soil Productivity Study sites. Samples were collected more than ten years after harvesting and replanting and were analyzed via shotgun metagenomics...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453228/targeted-profiling-of-a-thaliana-sub-proteomes-illuminates-new-co-and-post-translationally-n-terminal-myristoylated-proteins
#11
Wojciech Majeran, Jean-Pierre Le Caer, Lalit Ponnala, Thierry Meinnel, Carmela Giglione
N-terminal myristoylation (MYR) is a biologically important co-translational protein lipidation. MYR is difficult to detect in vivo and challenging to predict in silico. We developed a targeted proteomics strategy to identify the Arabidopsis thaliana myristoylated (MYRed) proteome in specific sub-cellular compartments. This global profiling approach allowed us to: (i) identify one third of all open reading frames of the A. thaliana proteome including 54% of the predicted myristoylome; and (ii) establish the first comprehensive plant myristoylome, featuring direct evidence of MYR in 72 proteins...
February 16, 2018: Plant Cell
https://www.readbyqxmd.com/read/29453220/identification-of-novel-fusion-transcripts-in-multiple-myeloma
#12
Mingxuan Lin, Peak Ling Lee, Lily Chiu, Constance Chua, Kenneth H K Ban, Adeline H F Lin, Zit Liang Chan, Tae-Hoon Chung, Benedict Yan, Wee-Joo Chng
AIMS: Multiple myeloma (MM) is a heterogeneous disease characterised by genetically complex abnormalities. The classical mutational spectrum includes recurrent chromosomal aberrations and gene-level mutations. Recurrent translocations involving the IGH gene such as t(11;14), t(4;14) and t(14;16) are well known. However, the presence of complex genetic abnormalities raises the possibility that fusions other than the recurrent IGH translocations exist. We therefore employed a targeted RNA-sequencing panel to identify novel putative fusions in a local cohort of MM...
February 16, 2018: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29453107/body-fluid-identification-using-a-targeted-mrna-massively-parallel-sequencing-approach-results-of-a-euroforgen-ednap-collaborative-exercise
#13
S Ingold, G Dørum, E Hanson, A Berti, W Branicki, P Brito, P Elsmore, K B Gettings, F Giangasparo, T E Gross, S Hansen, E N Hanssen, M-L Kampmann, M Kayser, F-X Laurent, N Morling, A Mosquera-Miguel, W Parson, C Phillips, M J Porto, E Pośpiech, A D Roeder, P M Schneider, K Schulze Johann, C R Steffen, D Syndercombe-Court, M Trautmann, M van den Berge, K J van der Gaag, J Vannier, V Verdoliva, A Vidaki, C Xavier, J Ballantyne, C Haas
In a previous study we presented an assay for targeted mRNA sequencing for the identification of human body fluids, optimised for the Illumina MiSeq/FGx MPS platform. This assay, together with an additional in-house designed assay for the Ion Torrent PGM/S5 platform, was the basis for a collaborative exercise within 17 EUROFORGEN and EDNAP laboratories, in order to test the efficacy of targeted mRNA sequencing to identify body fluids. The task was to analyse the supplied dried body fluid stains and, optionally, participants' own bona fide or mock casework samples of human origin, according to specified protocols...
January 31, 2018: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29453081/sluggish-dorsally-driven-inhibition-of-return-during-orthographic-processing-in-adults-with-dyslexia
#14
S Franceschini, S Mascheretti, S Bertoni, V Trezzi, C Andreola, S Gori, A Facoetti
Dyslexia (D) is a neurodevelopmental reading disorder characterized by phonological and orthographic deficits. Before phonological decoding, reading requires a specialized orthographic system for parallel letter processing that assigns letter identities to different spatial locations. The magnocellular-dorsal (MD) stream rapidly process the spatial location of visual stimuli controlling visuo-spatial attention. To investigate the visuo-spatial attention efficiency during orthographic processing, inhibition of return (IOR) was measured in adults with and without D in a lexical decision task...
February 13, 2018: Brain and Language
https://www.readbyqxmd.com/read/29453035/changes-in-correlation-characteristics-of-time-consumption-and-mind-reading-performance-in-pre-onset-and-post-onset-psychosis
#15
TianHong Zhang, LiHua Xu, HuiRu Cui, YingYing Tang, YanYan Wei, XiaoChen Tang, XiaoHua Liu, XinMei Cao, ChunBo Li, JiJun Wang
There is a strong correlation between neurocognition and social cognition. However, none of these studies have examined the key role of time consumption during social cognition tasks. Participants included 84 individuals with clinical high risk of psychosis (CHR), 95 healthy controls (HC), and 66 case controls (schizophrenia patients, SZ), who were assessed through the Reading-Mind-in-Eyes Tasks (RMET) with computerized recording of the response time (RT). Neurocognitive tests were also performed for the HC and CHR groups...
February 7, 2018: Psychiatry Research
https://www.readbyqxmd.com/read/29452931/identification-of-novel-uncertainty-factors-and-thresholds-of-toxicological-concern-for-health-hazard-and-risk-assessment-application-to-cleaning-product-ingredients
#16
REVIEW
Zhen Wang, W Casan Scott, E Spencer Williams, Michael Ciarlo, Paul C DeLeo, Bryan W Brooks
Uncertainty factors (UFs) are commonly used during hazard and risk assessments to address uncertainties, including extrapolations among mammals and experimental durations. In risk assessment, default values are routinely used for interspecies extrapolation and interindividual variability. Whether default UFs are sufficient for various chemical uses or specific chemical classes remains understudied, particularly for ingredients in cleaning products. Therefore, we examined publicly available acute median lethal dose (LD50), and reproductive and developmental no-observed-adverse-effect level (NOAEL) and lowest-observed-adverse-effect level (LOAEL) values for the rat model (oral)...
February 13, 2018: Environment International
https://www.readbyqxmd.com/read/29452918/predictors-of-safe-discharge-for-pediatric-drowning-patients-in-the-emergency-department
#17
Courtney E Brennan, Travis K F Hong, Vincent J Wang
OBJECTIVES: The purpose of this study is to determine if stable, well-appearing, drowning patients who have normal age-adjusted vital signs and pulse oximetry upon arrival to the emergency department may be safely discharged without a prolonged observation period. METHODS: Medical records were retrospectively reviewed for drowning patients presenting to a single pediatric emergency department from 1995 to 2014. Data were collected on vital signs and pulse oximetry at presentation, chest x-ray results, disposition and complications for each encounter...
January 31, 2018: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29452361/iui-review-and-systematic-assessment-of-the-evidence-that-supports-global-recommendations
#18
Ben Cohlen, Aartjan Bijkerk, Sheryl Van der Poel, Willem Ombelet
BACKGROUND: IUI with or without ovarian stimulation (OS) has become a first-line treatment option for many infertile couples, worldwide. The appropriate treatment modality for couples and their clinical management through IUI or IUI/OS cycles must consider maternal and perinatal outcomes, most notably the clinical complication of higher-order multiple pregnancies associated with IUI-OS. With a current global emphasis to continue to decrease maternal and perinatal mortality and morbidity, the World Health Organization (WHO) had established a multi-year project to review the evidence for the establishment of normative guidance for the implementation of IUI as a treatment to address fertility problems, and to consider its cost-effectiveness for lower resource settings...
February 14, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/29451882/de-novo-transcriptomic-analysis-of-leaf-and-fruit-tissue-of-cornus-officinalis-using-illumina-platform
#19
Dian-Yun Hou, Lin-Chun Shi, Meng-Meng Yang, Jiong Li, Shuang Zhou, Hong-Xiao Zhang, Hua-Wei Xu
Cornus officinalis is one of the most widely used medicinal plants in China and other East Asian countries to cure diseases such as liver, kidney, cardiovascular diseases and frequent urination for thousands of years. It is a Level 3 protected species, and is one of the 42 national key protected wild species of animals and plants in China. However, the genetics and molecular biology of C. officinalis are poorly understood, which has hindered research on the molecular mechanism of its metabolism and utilization...
2018: PloS One
https://www.readbyqxmd.com/read/29451779/real-time-intrinsic-fluorescence-visualisation-and-sizing-of-proteins-and-protein-complexes-in-microfluidic-devices
#20
Pavan Kumar Challa, Quentin Peter, Maya A Wright, Yuewen Zhang, Kadi-Liis Saar, Jacqueline A Carozza, Justin L P Benesch, Tuomas P J Knowles
Optical detection has become a convenient and scalable approach to read out information from microfluidic systems. For the study of many key biomolecules, however, including peptides and proteins, which have low fluorescence emission efficiencies at visible wavelengths, this approach typically requires labelling of the species of interest with extrinsic fluorophores to enhance the optical signal obtained -- a process which can be time-consuming, requires purification steps, and has the propensity to perturb the behaviour of the systems under study due to interactions between the labels and the analyte molecules...
February 16, 2018: Analytical Chemistry
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