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https://www.readbyqxmd.com/read/28088477/selected-scorpion-toxin-exposures-induce-cytokine-release-in-human-peripheral-blood-mononuclear-cells
#1
Gerardo Corzo, Gerardo Pavel Espino-Solis
A cytokine screening on human peripheral blood mononuclear cells (PBMCs) stimulated with selected scorpion toxins (ScTx's) was performed in order to evaluate their effect on human immune cells. The ScTx's chosen for this report were three typical buthid scorpion venom peptides, one with lethal effects on mammals Centruroides suffussus suffusus toxin II (CssII), another, with lethal effects on insects and crustaceans Centruroides noxius toxin 5 (Cn5), and one more without lethal effects Tityus discrepans toxin (Discrepin)...
January 11, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28081511/spatiotemporal-distributions-of-butyltin-compounds-in-various-intertidal-organisms-along-the-samcheok-and-tongyeong-coasts-of-korea
#2
Taewoo Kim, Seungyeon Jeon, Seongjin Hong, Sung Joon Song, Bong-Oh Kwon, Jongseong Ryu, Jong Seong Khim
Thirteen years ago, the Korean Government introduced a regulation prohibiting the use of tributyltin (TBT), which was a component of antifouling paints. A subsequent decline in the concentration of butyltins (BTs) was recorded in seawater and the sediment, however, the current concentration of BTs in biota has not been well documented. The spatiotemporal distribution and concentration of BTs was recorded in biota from 2013 to 2015 along the coasts of Samcheok and Tongyeong using GS/MSD analysis. Crustaceans contained the greatest concentrations of BTs, followed by gastropods, fishes, and bivalves...
January 2, 2017: Chemosphere
https://www.readbyqxmd.com/read/28079015/chemometrics-expertise-in-the-links-between-ecotoxicity-and-physicochemical-features-of-silver-nanoparticles-environmental-aspects
#3
Miroslava A Nedyalkova, Borjana V Donkova, Vasil D Simeonov
Studies of the ecotoxicological aspects of nanomaterials in aquatic environments are scarce. Given the growing varietyof nanoparticles (NPs), along with the diversity of aquatic species and environments, the key to promoting sound risk assessment in nanoecotoxicology is understanding the mechanisms that govern the fate of NPs in aquatic environments and their behavior at the NP-biota interface. In this paper, data collected from the literature on ecotoxicological effects observed in aquatic species is discussed and analyzed using multivariate statistics techniques...
January 12, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28077333/molecular-cloning-characterization-and-expression-analysis-of-two-juvenile-hormone-esterase-like-carboxylesterase-cdnas-in-chinese-mitten-crab-eriocheir-sinensis
#4
Yu Xu, Muzi Zhao, Yanfei Deng, Yuanjie Yang, Xuguang Li, Quanping Lu, Jiachun Ge, Jianlin Pan, Zhiqiang Xu
Precise regulation of methyl farnesoate (MF) titer is of prime importance throughout the crustacean life-cycle. Although the synthetic pathway of MF is well-documented, little is known about its degradation and recycling in crustaceans. Juvenile hormone esterase-like (JHE-like) carboxylesterase (CXE) is a key enzyme in MF degradation, thus playing a significant role in regulating the MF titer. We identified and characterized two cDNAs, Es-CXE1 and Es-CXE2, encoding JHE-like CXEs in Chinese mitten crab. Full-length cDNAs of Es-CXE1 and Es-CXE2 encode proteins composed of 584 and 597 amino acids, respectively, both of which contain a typical carboxylesterase domain...
January 8, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28076454/a-broad-ph-range-and-processive-chitinase-from-a-metagenome-library
#5
S S Thimoteo, A Glogauer, H Faoro, E M de Souza, L F Huergo, B M Moerschbacher, F O Pedrosa
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene named metachi18A. The metachi18A gene was subcloned and overexpressed in Escherichia coli BL21 and the MetaChi18A chitinase was purified by affinity chromatography as a 6xHis-tagged fusion protein...
January 5, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28069534/genetic-spatial-structure-of-an-anchialine-cave-annelid-indicates-connectivity-within-but-not-between-islands-of-the-great-bahama-bank
#6
Brett C Gonzalez, Alejandro Martínez, Elizabeth Borda, Thomas M Iliffe, Diego Fontaneto, Katrine Worsaae
Land-locked anchialine blue holes are karstic sinkholes and caves with tidally influenced, vertically stratified water bodies that harbor endemic fauna exhibiting variable troglomorphic features. These habitats represent island-like systems, which can serve to elucidate evolutionary and biogeographic processes at local scales. We investigated whether the 'continuous spelean corridor' hypothesis may elucidate the biogeographical distributions of the stygobitic annelid Pelagomacellicephala iliffei (Polynoidae) collected from the Great Bahama and Caicos Banks of the Bahamas Archipelago...
January 6, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28069434/identification-and-functional-analysis-of-a-tep-gene-from-a-crustacean-reveals-its-transcriptional-regulation-mediated-by-nf-%C3%AE%C2%BAb-and-jnk-pathways-and-its-broad-protective-roles-against-multiple-pathogens
#7
Chaozheng Li, Haoyang Li, Bang Xiao, Yonggui Chen, Sheng Wang, Kai Lǚ, Bin Yin, Sedong Li, Jianguo He
Thioester-containing proteins (TEPs) are present in a wide range of species from deuterostomes to protostomes and are thought to be involved in innate immunity. In the current study, a TEP gene homologous to insect TEPs (iTEP) from the crustacean Litopenaeus vannamei, named LvTEP1, is cloned and functionally characterized. The open reading frame (ORF) of LvTEP1 is 4383 bp in length, encoding a polypeptide of 1460 amino acids with a calculated molecular weight of 161.1 kDa LvTEP1, which is most similar to other TEPs from insects, contains some conserved sequence features, including a N-terminal signal peptide, a canonical thioester (TE) motif, and a C-terminal distinctive cysteine signature...
January 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28069422/identification-of-ecdysteroid-receptor-mediated-signaling-pathways-in-the-hepatopancreas-of-the-red-swamp-crayfish-procambarus-clarkii
#8
Baojian Zhu, Lin Tang, Yingying Yu, Huimin Yu, Lei Wang, Cen Qian, Guoqing Wei, Chaoliang Liu
The hepatopancreas of crustaceans plays an important role in lipid and carbohydrate metabolism, digestion of food, and biogenesis. In this study, the hepatopancreas transcriptome from the red crayfish Procambarus clarkii was characterized for the first time using high-throughput sequencing, producing approximately 41.4 million reads were obtained. After de novo assembly, 57,363 unigenes with an average length of 725bp were identified, Gene Ontology analysis categorized 22,580 as being involved in biological processes, among which metabolic process and cellular process groups were the most highly enriched...
January 6, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28068582/subcellular-distribution-and-trophic-transfer-of-pb-from-bivalves-to-the-common-prawn-palaemon-serratus
#9
Paula Sánchez-Marín, Ricardo Beiras
The edible clam Dosinia exoleta has been reported to accumulate high contents of lead (Pb) in soft tissues disregarding the levels of Pb in the environment. This is due to the retention of Pb in the form of metal rich granules (MRG) in their kidneys throughout the mollusc lifespan. The potential for trophic transfer of Pb in this form to predators is expected to be low, since metals in the form of MRG are generally supposed to be trophically unavailable, but this assumption is based on studies with other metals (Ag, Cd, Cu or Zn) and has not been demonstrated with Pb until now...
January 6, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28066712/de-novo-transcriptome-assembly-of-shrimp-palaemon-serratus
#10
Alejandra Perina, Ana M González-Tizón, Iago F Meilán, Andrés Martínez-Lage
: The shrimp Palaemon serratus is a coastal decapod crustacean with a high commercial value. It is harvested for human consumption. In this study, we used Illumina sequencing technology (HiSeq 2000) to sequence, assemble and annotate the transcriptome of P. serratus. RNA was isolated from muscle of adults individuals and, from a pool of larvae. A total number of 4 cDNA libraries were constructed, using the TruSeq RNA Sample Preparation Kit v2. The raw data in this study was deposited in NCBI SRA database with study accession number of SRP090769...
March 2017: Genomics Data
https://www.readbyqxmd.com/read/28065701/recent-progress-toward-the-identification-of-anti-viral-immune-mechanisms-in-decapod-crustaceans
#11
Chris Hauton
The sustainable intensification of crustacean aquaculture, which is dominated by the farming of penaeid shrimp species, continues to be beset by viral disease outbreaks. Despite this, reports exist of differential susceptibility to viral infection between different shrimp species and populations, and between shrimp and other decapod crustaceans. These reports have, in part, provided the motivation to identify key mechanisms of antiviral resistance, or refractivity, in commercially-important species. Within the last decade these studies have created significant advances in our understanding of host virus interactions in decapod models...
January 5, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28064074/effect-of-humic-acid-on-the-toxicity-of-bare-and-capped-zno-nanoparticles-on-bacteria-algal-and-crustacean-systems
#12
K Akhil, S Sudheer Khan
Zinc oxide nanoparticles are one of the most extensively used nanoparticles in various commercial products. Depending on the purpose, the particles are coated with various agents including poly vinyl pyrrolidone, poly vinyl alcohol, ethylene glycol etc. As the particles are heavily used, the chance of the particles to get run off into the environment is very high. The study explains the difference in toxicity of bare and capped zinc oxide nanoparticles under various environmental conditions including humic acid and visible light against bacterial, algal and crustacean system...
December 19, 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28057344/depuration-of-perfluoroalkyl-substances-from-the-edible-tissues-of-wild-caught-invertebrate-species
#13
Matthew D Taylor, Karl C Bowles, Daniel D Johnson, Natalie A Moltschaniwskyj
Detection and quantification of poly- and perfluoroalkyl substances (PFASs) in aquatic organisms is increasing, particularly for saltwater species. Depuration can remove PFASs from the tissues of some species once they are removed from the contaminant source, but it is not known if this process occurs for saltwater crustaceans. Such information is important for managing human health risks for exploited migratory species following exposure. We present the results of a depuration trial for School Prawn (Metapenaeus macleayi) and Mud Crab (Scylla serrata), two commercially important crustaceans in Australia...
January 2, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28054448/taxonomic-research-on-deep-sea-macrofauna-in-the-south-china-sea-using-the-chinese-deep-sea-submersible-jiaolong
#14
REVIEW
Xinzheng Li
This paper reviews the taxonomic and biodiversity studies of the deep-sea invertebrates in the South China Sea based on the samples collected by the Chinese manned deep-sea submersible Jiaolong. To date, six new species were described, including the sponges Lophophysema eversa Gong, Li & Qiu, 2014, Saccocalyx microhexactin Gong, Li & Qiu, 2015, and Semperella jiaolongae Gong, Li & Qiu, 2015 as well as the crustaceans Uroptychus jiaolongae Dong & Li, 2015, Uroptychus spinulosus Dong & Li, 2015 and Globospongicola jiaolongi Jiang, Kou & Li, 2015, and some newly recorded species from the South China Sea were reported...
January 5, 2017: Integrative Zoology
https://www.readbyqxmd.com/read/28043021/toxic-effects-of-diclofenac-on-life-history-parameters-and-the-expression-of-detoxification-related-genes-in-daphnia-magna
#15
Yang Liu, Lan Wang, Benben Pan, Chao Wang, Shuang Bao, Xiangping Nie
Diclofenac (DCF), as a widely used drug, has been detected in various environmental media such as municipal wastewater effluent. However, there is little information on the effects of DCF on freshwater invertebrates potentially exposing to its residues in surface water. In the present study, we investigated the toxic effects of DCF on the physiological parameters (e.g., survival, growth rate, and reproduction) of a crustacean, Daphnia magna, via a 21-d chronic toxicity test, and we also evaluated the effects of DCF on the expression of the genes related to the detoxification metabolism, growth, development and reproduction (e...
December 26, 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/28035609/high-levels-of-mercury-in-wetland-resources-from-three-river-basins-in-ghana-a-concern-for-public-health
#16
Francis Gbogbo, Samuel D Otoo, Robert Quaye Huago, Obed Asomaning
Crustaceans, mollusks, and fish are wetland resources that constitute an important source of protein and foreign exchange for the Ghanaian population, and many species of these are sold in the open market and restaurants, yet studies on their heavy metal contents are generally scarce. This paper evaluates the levels of mercury in five species of crustaceans, two species of mollusks, and ten species of fish inhabiting three river basins with different catchment activities in Ghana. These include the Ankobra Basin, characterized with mining and agriculture, Densu Basin, associated with urban waste discharges and agriculture, and Lower Volta River Basin, associated with agricultural activities...
December 29, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28032356/use-of-computer-assisted-image-analysis-for-semi-quantitative-histology-of-the-hepatopancreas-in-whiteleg-shrimp-penaeus-vannamei-boone
#17
F Cervellione, C McGurk, T Berger Eriksen, W Van den Broeck
Despite the increasing use of novel molecular techniques in pathology, histology remains the standard method for monitoring tissue alterations and for assessing pathology. Histopathological evaluation is generally laborious and subjective with risk of discrepancies in semi-quantitative scoring between pathologists. In contrast, computer-assisted image analysis (CAIA) is potentially faster, more objective and thus suitable for routine screening. Limited research has been carried out on CAIA in crustacean histopathology, and the methods described were not fully automated...
December 29, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28028472/candidate-pathogenicity-islands-in-the-genome-of-candidatus-rickettsiella-isopodorum-an-intracellular-bacterium-infecting-terrestrial-isopod-crustaceans
#18
YaDong Wang, Christopher Chandler
The bacterial genus Rickettsiellabelongs to the order Legionellales in the Gammaproteobacteria, and consists of several described species and pathotypes, most of which are considered to be intracellular pathogens infecting arthropods. Two members of this genus, R. grylliand R. isopodorum, are known to infect terrestrial isopod crustaceans. In this study, we assembled a draft genomic sequence for R. isopodorum, and performed a comparative genomic analysis with R. grylli. We found evidence for several candidate genomic island regions in R...
2016: PeerJ
https://www.readbyqxmd.com/read/28027402/shellfish-allergens-tropomyosin-and-beyond
#19
Margaretha A Faber, Mariona Pascal, Ouiam El Kharbouchi, Vito Sabato, Margo M Hagendorens, Ine I Decuyper, Chris H Bridts, Didier G Ebo
IgE-mediated shellfish allergy constitutes an important cause of food-related adverse reactions. Shellfish are classified into molluscs and crustaceans, the latter belonging to the class of arthropoda. Among crustaceans, shrimps are the most predominant cause of allergic reactions and thus more extensively studied. Several major and minor allergens have been identified and cloned. Amongst them invertebrate tropomyosin, arginine kinase, myosin light chain, sarcoplasmic calcium-binding protein and hemocyanin are the most relevant...
December 27, 2016: Allergy
https://www.readbyqxmd.com/read/28026008/cyprinid-herpesvirus-3-as-a-potential-biological-control-agent-for-carp-cyprinus-carpio-in-australia-susceptibility-of-non-target-species
#20
K A McColl, A Sunarto, J Slater, K Bell, M Asmus, W Fulton, K Hall, P Brown, D Gilligan, J Hoad, L M Williams, M St J Crane
Carp (Cyprinus carpio L.) is a pest species in Australian waterways, and cyprinid herpesvirus 3 (CyHV-3) is being considered as a potential biological control (biocontrol) agent. An important consideration for any such agent is its target specificity. In this study, the susceptibility to CyHV-3 of a range of non-target species (NTS) was tested. The NTS were as follows: 13 native Australian, and one introduced, fish species; a lamprey species; a crustacean; two native amphibian species (tadpole and mature stages); two native reptilian species; chickens; and laboratory mice...
December 27, 2016: Journal of Fish Diseases
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