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https://www.readbyqxmd.com/read/29353445/hypermethylation-of-nf-%C3%AE%C2%BAb-activating-protein-like-nkapl-promoter-in-hepatocellular-carcinoma-suppresses-its-expression-and-predicts-a-poor-prognosis
#1
Patrick Kwok Shing Ng, Carol Po Ying Lau, Emily Kai Yee Lam, Sheila Sai Kam Li, Vivian Wai Yan Lui, Winnie Yeo, Yuen Keng Ng, Paul Bo San Lai, Stephen Kwok Wing Tsui
BACKGROUND AND AIM: Hepatocellular carcinoma (HCC) is a complicated disease with low survival rate partially due to frequent recurrence and no efficient therapy. Promoter hypermethylation of tumor suppressor genes has been demonstrated as one of the molecular mechanisms contributing to tumorigenesis and progression in HCC. This study aims to investigate regulation of NKAPL expression by promoter methylation and its clinical relevance as a biomarker for HCC. METHODS: We measured mRNA expression of NKAPL in 5 HCC cell lines and a cohort of 62 pairs of primary HCC tumor and their adjacent non-cancer liver tissues...
January 20, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29353343/the-decreased-expression-of-stat3-and-p-stat3-in-preeclampsia-like-rat-placenta
#2
Zhan Zhang, Xiaofang Wang, Jinming Wang, Linlin Zhang
This study aims to investigate the expression of Stat3 and p-Stat3 in the placenta of a preeclampsia-like rat model induced by Nωnitro-L-arginine methyl ester (L-NAME). Two-to three-month-old (20 males, 40 females) Sprague-Dawley rats were used in this study. After conception was confirmed by vaginal smears, on the thirteenth day of pregnancy, the animals were allocated into two groups: control (0.9% NaCl administered) group and L-NAME (75 mg/kg) group. After the treatment of L-NAME, there was a significant increase in systolic blood pressure (SBP) levels in the L-NAME group (148...
January 20, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29353209/mir-182-promotes-prostate-cancer-progression-through-activating-wnt-%C3%AE-catenin-signal-pathway
#3
Dawei Wang, Guoliang Lu, Yuan Shao, Da Xu
Although prostate cancer can be surgical excised and effectively treated by androgen-deprivation therapy, radiotherapy, or chemotherapy, management of patients with advanced or drug-resistance prostate cancer stills remains a big trouble. Accumulated evidence indicated that miR-182 and Wnt/β-catenin function as tumor oncogene in the progression of a variety of tumors. However, little is known about how miR-182 regulates β-catenin signal molecular and impacts on the tumorigenesis of human prostate cancer. In this study, employing the analyses of qRT-PCR, we found that prostate cancer tissues expressed much more miR-182 than non-cancer tissues did...
January 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29353145/extremely-low-frequency-electromagnetic-field-emf-generates-alterations-in-the-synthesis-and-secretion-of-oestradiol-17%C3%AE-e2-in-uterine-tissues-an-in%C3%A2-vitro-study
#4
Anna Koziorowska, Ewa Monika Waszkiewicz, Maria Romerowicz-Misielak, Kamila Zglejc-Waszak, Anita Franczak
An electromagnetic field (EMF) of extremely low frequency may affect physiological processes in mammals. The aim of the present study was to determine the effect of an EMF on the synthesis and secretion of oestradiol-17β (E2) in the porcine uterus. Endometrial and myometrial slices were harvested on days 12-13 of the oestrous cycle and exposed in vitro to an EMF (50 and 120 Hz, 8 mT) for 2 and 4 h in the presence or absence of progesterone (P4). Subsequently, the incubation media were used to determine the concentration of E2 with RIA...
January 9, 2018: Theriogenology
https://www.readbyqxmd.com/read/29353142/mirna-185-regulates-the-vegfa-signaling-pathway-in-dairy-cows-with-retained-fetal-membranes
#5
REVIEW
C Y Zheng, X Zou, H J Lin, B C Zhao, M L Zhang, C H Luo, S X Fu
Retention of fetal membranes (RFM) of cows is an important reproductive disturbance, and is related to miRNAs. Vascular endothelial growth factor (VEGF)A, regulated by miRNA-185, can activate arachidonic acid (ARA) release via the VEGFA signaling pathway, which influences RFM. The aim of this study was to explore the pathogenic mechanism of RFM by investigating the regulatory relationship between miRNA-185 and the VEGFA signaling pathway. Serum samples of healthy Holstein dairy cows (n = 20) and RFM cows (n = 12), with a similar age, parity, weight, and milk yield, were collected to detect VEGFA and ARA concentrations at 6, 12, and 24 h after calving...
January 9, 2018: Theriogenology
https://www.readbyqxmd.com/read/29353067/antinociception-of-the-spirocyclopiperazinium-salt-compound-lxm-15-via-activating-%C3%AE-7-nachr-and-m4-machr-and-inhibiting-camkii%C3%AE-camp-creb-cgrp-signalling-pathway-in-mice
#6
Hua Yang, Qi Sun, Yingying Liang, Yimin Jiang, Runtao Li, Jia Ye
The aim of this study was to investigate the analgesic effect of the spirocyclopiperazinium salt compound LXM-15 by intragastric administration in thermal and chemical pain models and further to elucidate the possible molecular mechanisms. The results showed that LXM-15 exerted significant antinociception in hot-plate test, formalin test and acetic acid writhing test. Western blot analysis showed that LXM-15 significantly reduced the upregulation of phosphorylation of calcium/calmodulin -dependent protein kinaseⅡα (CaMKIIα) and cAMP response element-binding protein (CREB), and further decreased the elevation of calcitonin gene related peptide (CGRP) in the dorsal root ganglion (DRG) and spinal cord in mice...
January 15, 2018: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/29353027/estrogen-receptor-%C3%AE-is-involved-in-tamoxifen-neuroprotective-effects-in-a-traumatic-brain-injury-male-rat-model
#7
Sher-Wei Lim, T T Eric Nyam, Cho-Ya Hu, Chung-Ching Chio, Che-Chuan Wang, Jinn-Rung Kuo
PURPOSE: The aim of the present study is to determine the neuroprotective mechanisms of tamoxifen (TMX) during traumatic brain injury (TBI), especially the effects on estrogen receptor-α (ERα) expression, as well as neuroinflammatory associations. MATERIAL AND METHODS: Anesthetized male Sprague-Dawley rats were divided into four groups: sham-operated controls, sham-operated controls given TMX (1 mg/kg/per day) for 3 days, those given a vehicle solution immediately after TBI, and those given TMX (1 mg/kg/per day) for 3 days...
January 15, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29353005/cryptotanshinone-inhibits-prostaglandin-e2-production-and-cox-2-expression-via-suppression-of-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway-in-lps-stimulated-caco-2-cells
#8
Shu-Guang Cao, Hui Wang, Li-Miao Lin, Xuan-Ping Xia, Hao Wu
Crytotanshinone (CTN), one of the main constituents of Salvia miltiorrhiza, has been known to exhibit antioxdative, anti-inflammatory and other important therapeutic activities. The aim of this study was to evaluate the effect of CTN on prostaglandin E2 and COX-2 production in LPS-stimulated human intestinal cells (Caco-2 cells). Caco-2 cells were stimulated with LPS in the presence or absence of CTN. The production of prostaglandin E2 (PGE2) was detected by ELISA. The expression of COX-2 was detected by qRT-PCR and Western blot...
January 15, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29352962/improved-systemic-metabolism-and-adipocyte-biology-in-mir-150-knockout-mice
#9
Minsung Kang, Xiaobing Liu, Yuchang Fu, W Timothy Garvey
INTRODUCTION: Short non-coding micro-RNAs (miRNAs) are post-transcriptional factors that directly regulate protein expression by degrading or inhibiting target mRNAs; however, the role of miRNAs in obesity and cardiometabolic disease remains unclarified. Based on our earlier study demonstrating that miR-150 influences lipid metabolism, we have studied effects of miR-150 on systemic metabolism and adipocyte biology. MATERIALS AND METHODS: Metabolic phenotypes including body weight, food intake, body composition, glucose tolerance and insulin sensitivity were assessed in WT and global miR-150 KO male mice fed a high-fat diet...
January 15, 2018: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/29352846/the-janus-kinase-inhibitor-tofacitinib-inhibits-tnf-%C3%AE-induced-gliostatin-expression-in-rheumatoid-fibroblast-like-synoviocytes
#10
Yohei Kawaguchi, Yuko Waguri-Nagaya, Naoe Tatematsu, Yusuke Oguri, Masaaki Kobayashi, Masahiro Nozaki, Kiyofumi Asai, Mineyoshi Aoyama, Takanobu Otsuka
OBJECTIVES: Gliostatin (GLS) is known to have angiogenic and arthritogenic activity, and GLS expression levels in serum from patients with rheumatoid arthritis (RA) are significantly correlated with the disease activity. Tofacitinib is a novel oral Janus kinase (JAK) inhibitor and is effective in treating RA. However, the mechanism of action of tofacitinib in fibroblast-like synoviocytes (FLSs) has not been elucidated. The purpose of this study was to investigate the modulatory effects of tofacitinib on serum GLS levels in patients with RA and GLS production in FLSs derived from patients with RA...
January 15, 2018: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/29352646/synthesis-and-biological-evaluation-of-anti-cancer-agents-that-selectively-inhibit-her2-over-expressed-breast-cancer-cell-growth-via-down-regulation-of-her2-protein
#11
Anran Zhao, Qiaoyun Zheng, Cody M Orahoske, Nethrie D Idippily, Morgan M Ashcraft, Aicha Quamine, Bin Su
Compound JCC76 selectively inhibited the proliferation of human epidermal growth factor 2 (Her2) over-expressed breast cancer cells. In the current study, a ligand based structural optimization was performed to generate new analogs, and we identified derivatives 16 and 17 that showed improved activity and selectivity against Her2 positive breast cancer cells. A structure activity relationship (SAR) was summarized. Compounds 16 and 17 were also examined by western blot assay to check their effect on Her2 protein...
January 12, 2018: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29352519/nafamostat-mesilate-attenuates-inflammation-and-apoptosis-and-promotes-locomotor-recovery-after-spinal-cord-injury
#12
Hui-Quan Duan, Qiu-Li Wu, Xue Yao, Bao-You Fan, Hong-Yu Shi, Chen-Xi Zhao, Yan Zhang, Bo Li, Chao Sun, Xiao-Hong Kong, Xin-Fu Zhou, Shi-Qing Feng
AIM: Spinal cord injury (SCI) leads to severe neural damage for which there is currently no effective treatment. Exploration of the neuroprotective effect among clinically approved drugs will speed up clinical translation of SCI. Nafamostat mesilate (NM) as a synthetic serine protease inhibitor has been used clinically in pancreatitis treatments. However, its effectiveness in SCI is unknown. The aim of this study was to confirm the efficacy of NM in ameliorating SCI. METHODS: Intraperitoneal administration of NM was performed on a contusion SCI model in Wistar rat...
January 19, 2018: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/29352487/disaccharide-combinations-and-the-expression-of-enolase3-and-plasma-membrane-ca2-atpase-isoform-in-sturgeon-sperm-cryopreservation
#13
M D Xi, P Li, H Du, X M Qiao, Z G Liu, Q W Wei
Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus. We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A...
January 19, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29352348/metarhizium-anisopliae-s-l-modulation-of-lipid-metabolism-during-tick-infection-is-independent-of-ampk-and-erk-pathways
#14
Fillipe A Sá, Caio Junior B Coutinho-Rodrigues, Isabele C Angelo, Jéssica P Fiorotti, Georgia C Atella, Vânia Rita E P Bittencourt, Mário Alberto C Silva-Neto
Despite the importance of fat body in metabolism of arthropods, studies in ticks are scarce. This study evaluated the lipid composition and activation of extracellular signal-regulated protein kinase (ERK) and AMP-activated protein kinase (AMPK) enzymes in Rhipicephalus microplus fat body after infection with different isolates of the fungus Metarhizium anisopliae sensu lato (Metschnikoff, 1879) Sorokin, 1883. The isolates CG 32, GC 112, GC 148, GC 347, and GC 629 were inoculated as viable or non-viable conidia in the ticks...
January 19, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29352308/high-efficiency-production-of-human-serum-albumin-in-the-posterior-silk-glands-of-transgenic-silkworms-bombyx-mori-l
#15
Qiujie Qian, Zhengying You, Lupeng Ye, Jiaqian Che, Yiran Wang, Shaohua Wang, Boxiong Zhong
Human serum albumin (HSA) is an important biological preparation with a variety of biological functions in clinical applications. In this study, the mRNA of a fusion transposase derived from the pESNT-PBase plasmid and a pBHSA plasmid containing the HSA gene under the control of a fibroin light chain (FL) promoter were co-injected into fertilized eggs. Fifty-six transgenic silkworm pedigrees expressing theexogenous recombinant HSA (rHSA) in the posterior silk glands (PSGs) with stable inheritance were successfully obtained...
2018: PloS One
https://www.readbyqxmd.com/read/29352284/sensitive-detection-of-fluorescence-in-western-blotting-by-merging-images
#16
Yukari Kondo, Shinichiro Higa, Takeshi Iwasaki, Tomoya Matsumoto, Kazumitsu Maehara, Akihito Harada, Yoshihiro Baba, Masatoshi Fujita, Yasuyuki Ohkawa
The western blotting technique is widely used to analyze protein expression levels and protein molecular weight. The chemiluminescence method is mainly used for detection due to its high sensitivity and ease of manipulation, but it is unsuitable for detailed analyses because it cannot be used to detect multiple proteins simultaneously. Recently, more attention has been paid to the fluorescence detection method because it is more quantitative and is suitable for the detection of multiple proteins simultaneously...
2018: PloS One
https://www.readbyqxmd.com/read/29351801/high-expression-of-tmem40-is-associated-with-the-malignant-behavior-and-tumorigenesis-in-bladder-cancer
#17
Zhen-Fei Zhang, Han-Rong Zhang, Qing-Yan Zhang, Shu-Yu Lai, Yu-Zhen Feng, Yi Zhou, Si-Rong Zheng, Rong Shi, Jue-Yu Zhou
BACKGROUND: Bladder cancer (BCa) is one of the most common cancers in the urinary system among the world. Previous studies suggested that TMEM40 expression level was significantly associated with clinicopathological parameters including histological grade, clinical stage and pT status of bladder cancer. However, the molecular mechanism of TMEM40 in BCa remains poorly understood. METHODS: Real-time quantitative RT-PCR (qRT-PCR) and western blot (WB) were used to examine the expression levels of TMEM40 in BCa tissues, paired non-cancer tissues and cell lines...
January 19, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29351796/the-jun-mir-22-hur-regulatory-axis-contributes-to-tumourigenesis-in-colorectal-cancer
#18
Yanqing Liu, Xiaorui Chen, Rongjie Cheng, Fei Yang, Mengchao Yu, Chen Wang, Shufang Cui, Yeting Hong, Hongwei Liang, Minghui Liu, Chihao Zhao, Meng Ding, Wu Sun, Zhijian Liu, Feng Sun, Chenyu Zhang, Zhen Zhou, Xiaohong Jiang, Xi Chen
BACKGROUND: Colorectal cancer (CRC) is a severe health problem worldwide. Clarifying the mechanisms for the deregulation of oncogenes and tumour suppressors in CRC is vital for its diagnosis, treatment, prognosis and prevention. Hu antigen R (HuR), which is highly upregulated in CRC, functions as a pivotal oncogene to promote CRC progression. However, the underlying cause of its dysregulation is poorly understood. METHODS: In CRC tissue sample pairs, HuR protein levels were measured by Western blot and immunohistochemical (IHC) staining, respectively...
January 19, 2018: Molecular Cancer
https://www.readbyqxmd.com/read/29351758/autophagy-promotes-metastasis-and-glycolysis-by-upregulating-mct1-expression-and-wnt-%C3%AE-catenin-signaling-pathway-activation-in-hepatocellular-carcinoma-cells
#19
Qing Fan, Liang Yang, Xiaodong Zhang, Yingbo Ma, Yan Li, Lei Dong, Zhihong Zong, Xiangdong Hua, Dongming Su, Hangyu Li, Jingang Liu
BACKGROUND: Autophagy is a dynamic physiological process that can generate energy and nutrients for cell survival during stress. Autophagy can regulate the migration and invasive ability in cancer cells. However, the connection between autophagy and metabolism is unclear. Monocarboxylate transporter 1 (MCT1) plays an important role in lactic acid transport and H+ clearance in cancer cells, and Wnt/β-catenin signaling can increase cancer cell glycolysis. We investigated whether autophagy promotes glycolysis in hepatocellular carcinoma (HCC) cells by activating the Wnt/β-catenin signaling pathway, accompanied by MCT1 upregulation...
January 19, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29351747/combination-of-pelargonium-sidoides-and-coptis-chinensis-root-inhibits-nuclear-factor-kappa-b-mediated-inflammatory-response-in-vitro-and-in-vivo
#20
Sang Mi Park, Byung-Gu Min, Ji Yun Jung, Kyung Hwan Jegal, Chul Won Lee, Kwang Youn Kim, Young Woo Kim, Youn-Woong Choi, Il Je Cho, Sae Kwang Ku, Sang Chan Kim
BACKGROUND: Pelargonium sidoides (PS) and Coptis chinensis root (CR) have traditionally been used to treat various diseases, including respiratory and gastrointestinal infections, dysmenorrhea, and hepatic disorders. The present study was conducted to evaluate the anti-inflammatory effects of a combination of PS and CR in vitro and in vivo. METHODS: The in vitro effects of PS + CR on the induction of inflammation-related proteins were evaluated in lipopolysaccharide (LPS)-stimulated RAW 264...
January 19, 2018: BMC Complementary and Alternative Medicine
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