keyword
MENU ▼
Read by QxMD icon Read
search

Western blot

keyword
https://www.readbyqxmd.com/read/28102735/bulk-immunoassays-for-analysis-of-extracellular-vesicles
#1
Frank A W Coumans, Elmar L Gool, Rienk Nieuwland
There is increasing clinical interest in extracellular vesicles (EV) for diagnostic and treatment purposes. This review provides an overview of bulk immunoassays to analyse EV. Western blot and enzyme-linked immunosorbent assay are still the two predominant bulk immunoassays. Recently, new assays have become available that can detect exposure to EV concentrations that are up to 10,000-fold lower. This is advantageous for applications that detect rare EV. Other important parameters are the detectable concentration range, the required sample volume, whether simultaneous presence of different antigens on a single EV can be detected, size selectivity of each assay and practical considerations...
January 19, 2017: Platelets
https://www.readbyqxmd.com/read/28102559/effect-of-bone-like-hydroxyapatite-poly-amino-acid-loaded-with-rifapentine-microspheres-on-bone-and-joint-tuberculosis-in-vitro
#2
Yuwu Liu, Dianming Jiang
Rifapentine-loaded poly(lactic-co-glycolic acid) microspheres (RPMs)-loaded bone-like hydroxyapatite/poly amino acid (BHA/PAA) is effective in curing S. aureus-induced chronic osteomyelitis. This study continues to investigate the effect of RPM-loaded BHA/PAA on the bacterial growth of Mycobacterium tuberculosis (MTB), cell proliferation and differentiation in MTB H37Rv-infected MG63 cells. Furthermore, whether Wnt/β-catenin signaling pathway was activated by RPM-loaded BHA/PAA was explored. We found the bactec growth index of H37Rv was significantly inhibited by RPM-loaded BHA/PAA...
January 19, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28102508/simvastatin-promotes-hematoma-absorption-and-reduces-hydrocephalus-following-intraventricular-hemorrhage-in-part-by-upregulating-cd36
#3
Qianwei Chen, Xia Shi, Qiang Tan, Zhou Feng, Yuelong Wang, Qiaoying Yuan, Yihao Tao, Jianbo Zhang, Liang Tan, Gang Zhu, Hua Feng, Zhi Chen
We previously found that hematoma worsens hydrocephalus after intraventricular hemorrhage (IVH) via increasing iron deposition and aggravating ependymal cilia injury; therefore, promoting hematoma absorption may be a promising strategy for IVH. Recently, some investigations imply that simvastatin has the ability of accelerating hematoma absorption. Thus, this study was designed to examine the efficacy of simvastatin for IVH in rats. Intracerebral hemorrhage with ventricular extension was induced in adult male Sprague-Dawley rats after autologous blood injection...
January 19, 2017: Translational Stroke Research
https://www.readbyqxmd.com/read/28102235/clumping-factor-a-of-staphylococcus-aureus-interacts-with-annexina2-on-mammary-epithelial-cells
#4
Shoaib Ashraf, Jing Cheng, Xin Zhao
Staphylococcus aureus is one of major pathogens that can cause a series of diseases in different hosts. In the bovine, it mainly causes subclinical and contagious mastitis, but its mechanisms of infection are not fully understood. Considering the fact that virulence factors play key roles in interactions between the bacterium and host cells, this study aimed to identify if a binding partner of S. aureus clumping factor A (ClfA) exists on the bovine mammary epithelial cells. The ClfA protein was used as a bait to pull down lysates of cultured bovine mammary epithelial cells (MAC-T cells)...
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28101961/antibody-formation-towards-porcine-tissue-in-patients-implanted-with-crosslinked-heart-valves-is-directed-to-antigenic-tissue-proteins-and-%C3%AE-gal-epitopes-and-is-reduced-in-healthy-vegetarian-subjects
#5
Ulrike Böer, Falk F R Buettner, Ariane Schridde, Melanie Klingenberg, Samir Sarikouch, Axel Haverich, Mathias Wilhelmi
BACKGROUND: Glutaraldehyde-fixed porcine heart valves (ga-pV) are one of the most frequently used substitutes for insufficient aortic and pulmonary heart valves which, however, degenerate after 10-15 years. Yet, xeno-immunogenicity of ga-pV in humans including identification of immunogens still needs to be investigated. We here determined the immunogenicity of ga-pV in patients with respect to antibody formation, identity of immunogens and potential options to reduce antibody levels. METHODS: Levels of tissue-specific and anti-αGal antibodies were determined retrospectively in patients who received ga-pV for 51 months (n=4), 25 months (n=6) or 5 months (n=4) and compared to age-matched untreated subjects (n=10) or younger subjects with or without vegetarian diet (n=12/15)...
January 18, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28101799/antitumor-efficacy-of-slpi-promoter-controlled-expression-of-artificial-microrna-targeting-egfr-in-a-squamous-cell-carcinoma-cell-line
#6
Jia Chen, Shoude Zhang, Yi Lin, Beibei Yang, Jiang Cao
The purpose of this study was to develop a recombinant adenovirus with secretory leukoprotease inhibitor (SLPI) promoter-controlled expression for gene therapy of squamous cell carcinoma (SCC). An artificial microRNA targeting epidermal growth factor receptor (EGFR) was designed, and used to construct a replication-defective recombinant adenovirus with SLPI promoter-controlled expression. The silencing efficiency of this vector (Ad-SLPI-EGFRamiR) was detected in Hep-2 cells. Western blotting showed that the expression of 170 kD EGFR was significantly reduced in Hep-2 cells 72 h after infection with Ad-SLPI-EGFRamiR...
January 18, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28101778/eight-novel-mut-loss-of-function-missense-mutations-in-chinese-patients-with-isolated-methylmalonic-academia
#7
Lian-Shu Han, Zhuo Huang, Feng Han, Yu Wang, Zhu-Wen Gong, Xue-Fan Gu
BACKGROUND: Isolated methylmalonic acidemia is a rare autosomal recessive metabolic disorder mostly caused by mutations in the methylmalonyl coenzyme A mutase (MCM) gene (MUT). This study aimed to verify whether missense mutations in MUT in Chinese patients affect the stability and enzymatic activity of MCM. METHODS: Eight Chinese patients were identified with novel mutations. Plasmids carrying the wild-type and mutated MUT cDNA were constructed and transfected into HEK293T cells for functional analyses...
January 19, 2017: World Journal of Pediatrics: WJP
https://www.readbyqxmd.com/read/28101579/role-of-trna-selenocysteine-1-associated-protein-1-in-the-proliferation-and-apoptosis-of-cardiomyocyte%C3%A2-like-h9c2-cells
#8
Meng-Di Li, Wan-Peng Cheng, Min-Xia Shi, Tang-Dong Ge, Xiao-Lin Zheng, Dong-Yuan Wu, Xiao-Yan Hu, Jin-Cheng Luo, Feng-Lan Li, Hui Li
Transfer RNA selenocysteine 1 associated protein 1 (Trnau1ap) serves an essential role in the synthesis of selenoproteins, which have critical functions in numerous biological processes. Selenium deficiency results in a variety of diseases, including cardiac disease. However, the mechanisms underlying myocardial injury induced by selenium deficiency remain unclear. The present study examined the effects of Trnau1ap under‑ and overexpression in cardiomyocyte‑like H9c2 cells, by transfection with small interfering RNA and an overexpression plasmid, respectively...
February 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28101575/identification-of-potential-targets-for-differentiation-in-human-leukemia-cells-induced-by-diallyl-disulfide
#9
Hui Ling, Jie He, Hui Tan, Lan Yi, Fang Liu, Xiaoxia Ji, Youhua Wu, Haobin Hu, Xi Zeng, Xiaohong Ai, Hao Jiang, Qi Su
Diallyl disulfide (DADS) is a primary component of garlic, which has chemopreventive potential. We previously found that moderate doses (15-120 µM) of DADS induced apoptosis and G2/M phase cell cycle arrest. In this study, we observed the effect of low doses (8 µM) of DADS on human leukemia HL-60 cells. We found that DADS could inhibit proliferation, migration and invasion in HL-60 cells, and arrested cells at G0/G1 stage. Then, cell differentiation was displayed by morphologic observation, NBT reduction activity and CD11b evaluation of cytometric flow...
February 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28101569/resveratrol-inhibits-adventitial-fibroblast-proliferation-and-induces-cell-apoptosis-through-the-sirt1-pathway
#10
Lin Ling, Shaohua Gu, Yan Cheng
Atherosclerosis is one of the most important causes of cardiovascular disease and studies have showed that adventitial fibroblasts, which are considered to be the most common cell type of the vascular adventitia, are involved in the development of early atherosclerotic plaques. Resveratrol is a plant polyphenolic compound confirmed to have anti‑atherosclerotic and cardioprotective effects. The aim of the present study was to investigate the effects of resveratrol on adventitial fibroblasts in vitro and to clarify the underlying mechanism...
February 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28101473/purification-and-characterization-of-bovine-serum-albumin-using-chromatographic-method
#11
Sanaz Balkani, Sara Shamekhi, Ramin Raoufinia, Reza Parvan, Jalal Abdolalizadeh
Purpose: Albumin is an abundant protein of blood and has many biopharmaceutical applications. The aim of this study was to purify bovine serum albumin (BSA) using produced rabbit anti-BSA antibody. Methods: The polyclonal antibody was produced against the BSA in rabbits. Then, the pure BSA was injected to three white New Zealand rabbits. ELISA test was done to evaluate antibody production. After antibody purification,the purified antibody was attached to CNBr-activated sepharose and finally it was used for purification of albumin from bovine serum...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101463/development-of-a-novel-human-single-chain-antibody-against-egfrviii-antigen-by-phage-display-technology
#12
Leila Rahbarnia, Safar Farajnia, Hossein Babaei, Jafar Majidi, Bahman Akbari, Shiva Ahdi Khosroshahi
Purpose: EGFRvIII as the most common mutant variant of the epidermal growth factor receptor is resulting from deletion of exons 2-7 in the coding sequence and junction of exons 1 and 8 through a novel glycine residue. EGFRvIII is highly expressed in glioblastoma, carcinoma of the breast, ovary, and lung but not in normal cells. The aim of the present study was identification of a novel single chain antibody against EGFRvIII as a promising target for cancer therapy. Methods: In this study, a synthetic peptide corresponding to EGFRvIII protein was used for screening a naive human scFv phage library...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101343/effect-of-1-25-oh-2d3-on-transdifferentiation-of-rat-renal-tubular-epithelial-cells-induced-by-high-glucose
#13
Hongtao Hu, Shen Xu, Shuang Hu, Yue Gao, Hua Shui
Deficiency in vitamin D and its active metabolite is a characteristic of chronic kidney diseases (CKDs). Previous studies have reported that 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3], the active form of vitamin D, can attenuate renal interstitial fibrosis. The present study aimed to explore the effect of 1,25(OH)2D3 on the transdifferentiation of NRK-52E rat renal tubular epithelial cells (RTECs) induced by high glucose, as well as the expression of vitamin D receptor (VDR) and production of angiotensin (Ang) II...
December 2016: Biomedical Reports
https://www.readbyqxmd.com/read/28101246/detection-of-microrna-200b-may-predict-the-inhibitory-effect-of-gefitinib-on-non-small-cell-lung-cancer-and-its-potential-mechanism
#14
Zhiwu Liu, Liqiong Yao, Bangyun Tan, Li Li, Baojin Chen
The present study aimed to investigate the association and underlying mechanisms between microRNA-200b level and the inhibitory effect of gefitinib on non-small cell lung cancer. In total, 100 patients (43 males and 57 females; median age, 63 years) with advanced non-small cell lung cancer (NSCLC) were selected. All patients were administered with gefitinib orally (250 mg/day) and the effect of gefitinib was evaluated according to the Response Evaluation Criteria in Solid Tumors guidelines. Tumor tissue and plasma samples were collected prior to and subsequent to therapy...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28101245/apobec3b-expression-in-human-leptomeninges-and-meningiomas
#15
Mahlon D Johnson, Jay E Reeder, Mary O'Connell
Nucleic acid-editing enzymes of the apolipoprotein B mRNA-editing enzyme (APOBEC) family have been associated with somatic mutation in cancer. However, the role of APOBEC catalytic subunit 3B (APOBEC3B) editing in the pathogenesis of base substitutions in meningiomas is unknown. In the present study, the expression of APOBEC3B was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analyses in five fetal and one adult human leptomeninges and 38 meningiomas. Genomic DNA was sequenced using the Illumina Tru-Seq Cancer Panel...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28101239/naphthazarin-suppresses-cell-proliferation-and-induces-apoptosis-in-human-colorectal-cancer-cells-via-the-b-cell-lymphoma-2-b-cell-associated-x-protein-signaling-pathway
#16
Ai-Dong Chen, Hui Li, Yong-Chun Li, Hai Zeng
Colorectal cancer is the most common gastrointestinal cancer in the USA. Naphthazarin, one of the naturally available 1,4-naphthoquinone derivatives, is a natural bioactive molecule that exhibits an antitumor effect. To the best of our knowledge, this is the first study to investigate the anticancer effect of naphthazarin on cell proliferation and apoptosis in human SW480 colorectal cancer cells. In the present study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were performed to assess the effect of napthazarin on cell proliferation and cytotoxicity of SW430 cells, respectively...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28101238/diosmetin-triggers-cell-apoptosis-by-activation-of-the-p53-bcl-2-pathway-and-inactivation-of-the-notch3-nf-%C3%AE%C2%BAb-pathway-in-hepg2-cells
#17
Jie Qiao, Jie Liu, Kaiqiao Jia, Ning Li, Bin Liu, Qingyu Zhang, Runzhi Zhu
Diosmetin (DIOS), a flavonoid compound, is abundant in Citrus limon. Emerging studies have shown that DIOS is an effective compound implicated in multiple types of cancer. However, whether DIOS serves a role in hepatocellular carcinoma (HCC) is still obscure. HepG2 cells were used in the present study, and it was observed that DIOS exhibited antitumor activity against liver cancer cells. Western blotting was performed to evaluate cell apoptosis and survival-associated proteins, and the results demonstrated that DIOS treatment resulted in the activation of the p53-dependent apoptosis pathway...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28101236/identification-of-proteins-associated-with-pediatric-bilateral-wilms-tumor
#18
Zechen Yan, Qingjun Meng, Jinjian Yang, Junjie Zhang, Wei Zhao, Fei Guo, Dongjian Song, Yuxiao Zhan, Dandan Fan, Ruiyi Zhou, Shuqiang Zuo, Zehua Wang, Jiekai Yu, Shu Zheng, Jiaxiang Wang
Wilms tumor (WT) is the most common cancer that primarily develops in abdominal solid organ of children. It has no incipient symptom, and the most frequent symptoms are a painless, palpable abdominal mass. Proteomics technology was used to select the differentially expressed proteins of bilateral Wilms tumor (BWT). Ten serum samples of children with BWT were chosen, 20 serum samples of children with unilateral WT (UWT) and 20 serum samples of healthy children were selected, and proteomics technology was used to detect and collect data...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28101219/n-terminal-truncated-carboxypeptidase-e-expression-is-associated-with-poor-prognosis-of-lung-adenocarcinoma
#19
Jing Sun, Dawei Meng, Li Li, Xin Tian, Yunji Jia, Hongyue Wang, Huihui Yu, Tiemin Sun, Aibing Qu, Hui Shen, Jimin Bao, Guirong Zhang
Lung cancer is a malignant tumor with high morbidity and mortality rates. To date, no suitable molecular diagnostic tool to predict disease recurrence and metastasis has been identified. The current study aimed to evaluate the potential of N-terminal truncated carboxypeptidase E (CPEΔN) to predict the recurrence and metastasis of lung adenocarcinoma. Western blotting revealed the co-expression of CPE and CPEΔN in the surgically collected pathological and pericarcinoma tissues tissues of 62.1% (59/95) lung adenocarcinoma patients...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28101214/parp-1-may-be-involved-in-angiogenesis-in-epithelial-ovarian-cancer
#20
Wei Wei, Yan Li, Shuqing Lv, Cancan Zhang, Yongjie Tian
Poly (ADP-ribose) polymerase 1 (PARP-1) is involved in DNA repair and has been implicated in chemoresistance. The present study investigated whether PARP-1 promotes angiogenesis in ovarian cancer. PARP-1 and vascular endothelial growth factor A (VEGF-A) expression and CD34(+) microvascular density (MVD) were assessed using immunohistochemistry in 60 human epithelial ovarian cancer specimens. PARP-1 was stably knocked-down in SKOV3 cells using a specific small interfering RNA (siRNA); angiogenic capacity was assessed using the human umbilical vein endothelial cell (HUVEC) tubule formation assay; and PARP-1 and VEGF-A expression were examined by reverse transcription-quantitative polymerase chain reaction, western blotting and ELISA...
December 2016: Oncology Letters
keyword
keyword
23248
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"