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https://www.readbyqxmd.com/read/28726315/biomineralization-of-gold-by-mucor-plumbeus-the-progress-in-understanding-the-mechanism-of-nanoparticles-formation
#1
Irena Maliszewska, Włodzimierz Tylus, Izabela Pawlaczyk-Graja, Wojciech Pusz, Anna Baturo-Cieśniewska, Jacek Chęcmanowski, Bogdan Szczygieł
This contribution describes the deposition of gold nanoparticles by microbial reduction of Au(III) ions using the mycelium of Mucor plumbeus. Biosorption as the major mechanism of Au(III) ions binding by the fungal cells and the reduction of them to the form of Au(0) on/in the cell wall, followed by the transportation of the synthesized gold nanoparticles to the cytoplasm, is postulated. The probable mechanism behind the reduction of Au(III) ions is discussed, leading to the conclusion that this process is non enzymatic one...
July 20, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28718996/production-of-ligninolytic-enzymes-and-some-diffusible-antifungal-compounds-by-white-rot-fungi-using-potato-solid-wastes-as-the-sole-nutrient-source
#2
H Schalchli, E Hormazábal, O Rubilar, G Briceño, A Mutis, G J Zocolo, C Diez
AIMS: The aim of this study was to evaluate the synthesis of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using peels or discarded potato as the sole nutrient source. METHODS AND RESULTS: The strain Trametes hirsuta Ru-513 has been highlighted for its laccase activity (595 ± 33 U l(-1) ), which is able to decolourise 87% of an anthraquinone dye using potato peels as the sole nutritional support. A native polyacrylamide gel of laccase proteins showed the presence of two isoenzymes, corresponding to proteins of 56 and 67 kDa, which were detected by SDS-PAGE...
July 18, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28698667/phylogenetic-analysis-reveals-two-genotypes-of-the-emerging-fungus-mucor-indicus-an-opportunistic-human-pathogen-in-immunocompromised-patients
#3
Saad J Taj-Aldeen, Muna Almaslamani, Bart Theelen, Teun Boekhout
Mucormycosis is a rare fungal infection caused by Mucor indicus. Phylogenetic analysis of many M. indicus isolates, mainly sampled from different clinical and environmental specimens collected worldwide, revealed two genotypes, I and II, based on ITS and D1/D2 LSU rDNA sequences. A retrospective review of the literature revealed 13 cases. Eight (76.9%) patients had disseminated infections, and the overall mortality rate was 30.7%. A pulmonary infection caused by M. indicus genotype I in a liver transplant recipient was disseminated to include the skin and was successfully treated with liposomal amphotericin B and aggressive surgery...
July 12, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28687045/sageretia-thea-osbeck-modulated-biosynthesis-of-nio-nanoparticles-and-their-in-vitro-pharmacognostic-antioxidant-and-cytotoxic-potential
#4
Ali Talha Khalil, Muhammad Ovais, Ikram Ullah, Muhammad Ali, Zabta Khan Shinwari, Dilawar Hassan, Malik Maaza
NiO nanoparticles are biosynthesized using Sageretia thea (Osbeck.) aqueous leave extracts and their biological activities are reported. Nanoparticles (∼18 nm) were characterized through XRD, ATR-FTIR, EDS, SAED, HR-SEM/TEM and Raman spectroscopy. Antibacterial activity was investigated against six pathogenic bacterial strains (gram positive and gram negative) and their corresponding minimum inhibitory concentrations (MICs) were calculated. UV-exposed nanoparticles were investigated to have reduced MICs relative to the NiO nanoparticles have not been exposed to UV...
July 7, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28681286/bioremoval-of-arsenic-v-from-aqueous-solutions-by-chemically-modified-fungal-biomass
#5
J F Cárdenas-González, I Acosta-Rodríguez, Y Téran-Figueroa, A S Rodríguez-Pérez
The biosorption of arsenic (V) on nine chemically modified biomasses (with iron oxide coated) of mycelia fungi: Aspergillus flavus III, IV and V, Aspergillus fumigatus I-II, Paecilomyces sp., Cladosporium sp., Mucor sp-1 and 2 was studied in this work. This study provides evidence that the biomasses of the fungi A. flavus, IV, III and V, Paecilomyces sp., and A. fumigatus I were very efficient at removing 1 mg/L of the metal in solution, using atomic absorption spectroscopy (AAS), achieving the following percentage of removals: 97...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28667347/clinical-analysis-of-diabetic-combined-pulmonary-mucormycosis
#6
Yu-Hong Li, Peng Sun, Jian-Chao Guo
OBJECTIVE: To improve the understanding of diabetic combined pulmonary mucormycosis. METHODS AND RESULTS: The clinical data of twelve patients diagnosed as diabetic combined pulmonary mucormycosis were analyzed by the clinical manifestations, imaging features, diagnosis, treatment and prognosis of this disease. Patients from January 1999 to June 2015 in our hospital had poor blood glucose control and varying degrees of diabetic chronic complications with an on average of diabetes 8...
June 30, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28659561/detection-of-fungi-from-an-indoor-environment-using-loop-mediated-isothermal-amplification-lamp-method
#7
Takako Nakayama, Takashi Yamazaki, Ayaka Yo, Kazuya Tone, Mohamed Mahdi Alshahni, Ryuichi Fujisaki, Koichi Makimura
 Loop-mediated isothermal amplification (LAMP) is a useful DNA detection method with high specificity and sensitivity. The LAMP reaction is carried out within a short time at a constant temperature without the need for thermal cycling. We developed a LAMP primer set for detecting a wide range of fungi by aligning the sequences of the large subunit ribosomal RNA gene of Candida albicans (Ascomycota), Cryptococcus neoformans (Basidiomycota), and Mucor racemosus (Mucorales). The threshold of C. albicans rDNA as template with our LAMP primer set was in the range of 10-100 copies per a reaction...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28637506/improved-%C3%AE-linolenic-acid-production-in-mucor-circinelloides-by-homologous-overexpressing-of-delta-12-and-delta-6-desaturases
#8
Yao Zhang, Xiao Luan, Huaiyuan Zhang, Victoriano Garre, Yuanda Song, Colin Ratledge
BACKGROUND: γ-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways of some plant and microbial GLA have been deciphered, current understanding of the correlation between desaturases and GLA synthesis in oleaginous fungi is incomplete. In previous work, we found that a large amount of oleic acid (OA) had not been converted to linoleic acid (LA) or GLA in Mucor circinelloides CBS 277.49, which may be due to inadequate activities of the delta-12 or delta-6 desaturases, and thus leading to the accumulation of OA and LA...
June 21, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28621110/characterization-of-microbial-communities-in-chinese-rice-wine-collected-at-yichang-city-and-suzhou-city-in-china
#9
Yucai Lyu, Yanli Gong, Yajie Li, Zejiang Pan, Yi Yao, Ning Li, Jinling Guo, Dachun Gong, Yihong Ti, Caiyun Peng
Two typical microbial communities from Chinese rice wine fermentation collected in Yichang city and Suzhou city in China were investigated. Both communities could ferment glutinous rice to rice wine in 2 d. The sugar and ethanol contents were 198.67 and 14.47 mg/g, respectively, for rice wine from Yichang city, and 292.50 and 12.31 mg/g, respectively, for rice wine from Suzhou city. Acetic acid and lactic acid were the most abundant organic acids. Abundant fungi and bacteria were detected in both communities by high-throughput sequencing...
June 16, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28616054/an-investigation-on-non-invasive-fungal-sinusitis-molecular-identification-of-etiologic-agents
#10
Abdolrasoul Mohammadi, Seyed Mostafa Hashemi, Sayed Hamidreza Abtahi, Seyed Mohammad Lajevardi, Sahar Kianipour, Rasoul Mohammadi
BACKGROUND: Fungal sinusitis is increasing worldwide in the past two decades. It is divided into two types including invasive and noninvasive. Noninvasive types contain allergic fungal sinusitis (AFS) and fungus ball. AFS is a hypersensitivity reaction to fungal allergens in the mucosa of the sinonasal tract in atopic individuals. The fungus ball is a different type of noninvasive fungal rhinosinusitis which is delineated as an accumulation of debris and fungal elements inside a paranasal sinus...
2017: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/28597371/uptake-and-biotransformation-of-pure-commercial-microcystin-lr-versus-microcystin-lr-from-a-natural-cyanobacterial-bloom-extract-in-the-aquatic-fungus-mucor-hiemalis
#11
Maranda Esterhuizen-Londt, Stefanie Hertel, Stephan Pflugmacher
OBJECTIVES: To evaluate the remediation efficiency of Mucor hiemalis by comparing media elimination, uptake, and biotransformation of microcystin-LR with exposure to pure toxin versus a crude bloom extract. RESULTS: With exposure to the extract, the elimination rate of microcystin-LR from the media, which was 0.28 ng MC-LR l(-1) h(-1), was significantly higher compared to that achieved with exposure to the pure toxin (0.16 ng MC-LR l(-1) h(-1)) after 24 h...
June 8, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28596926/invasive-mucormycosis-induced-pneumopericardium-a-rare-cause-of-pneumopericardium-in-an-immunocompromised-patient
#12
Sana Khan, Muhammad Waqar Elahi, Waqas Ullah, Hafez Mohammad Ammar Abdullah, Ejaz Ahmad, Mayar Al Mohajer, Aneela Majeed
Mucor and Rhizopus cause life-threatening infections primarily involving the lungs and sinuses, which disseminate very rapidly by necrosis and infarction of the contiguous tissues. We present a case of a 64-year-old African American posttransplant patient who presented with a productive cough and weight loss. He had a past surgical history of renal transplant for renal cell carcinoma and was on dual immunosuppressive therapy, that is, mycophenolate and tacrolimus. During his hospital stay, he developed a pneumopericardium due to the direct extension of a lung lesion...
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/28584912/new-cryptotanshinone-derivatives-with-anti-influenza-a-virus-activities-obtained-via-biotransformation-by-mucor-rouxii
#13
Wenni He, Yao Li, Yuejie Qin, Xiaomei Tong, Zhijun Song, Yu Zhao, Ran Wei, Li Li, Huanqin Dai, Wenzhao Wang, Houwei Luo, Xin Ye, Lixin Zhang, Xueting Liu
This paper provides an efficient platform to diversify the structure and pharmaceutical potentials of known natural products. Seven metabolites were obtained via the biotransformation of cryptotanshinone by the fungus Mucor rouxii AS 3.3447, and assigned as 13R-14R-hydroxy-anhydride of 16R-cryptotanshinone (1), 1S-hydroxy-anhydride of 16R-cryptotanshinone (2), 1R-hydroxy-anhydride of 16R-cryptotanshinone (3), 3S-hydroxy-epicryptoacetalide (4), 3S-hydroxy-cryptoacetalide (5), epicryptoacetalide (6), and cryptoacetalide (7)...
June 5, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28560092/comparison-in-antioxidant-and-antitumor-activities-of-pine-polyphenols-and-its-seven-biotransformation-extracts-by-fungi
#14
Hui Li, Zhenyu Wang
Microbial transformation can strengthen the antioxidant and antitumor activities of polyphenols. Polyphenols contents, antioxidant and antitumor activities of pine polyphenols and its biotransformation extracts by Aspergillus niger, Aspergillus oryzae, Aspergillus carbonarius, Aspergillus candidus, Trichodermas viride, Mucor wutungkiao and Rhizopus sp were studied. Significant differences were noted in antioxidant and antitumor activities. The highest antioxidant activities in Trolox equivalent antioxidant capacity (TEAC), DPPH radical scavenging activity, superoxide anion radical scavenging activity, hydroxyl radical scavenging activity, reducing power assay and antitumor activity against LoVo cells were biotransformation extract of Aspergillus carbonarius (BAC), biotransformation extract of Mucor wutungkiao (BMW), biotransformation extract of Aspergillus carbonarius (BAC), biotransformation extract of Aspergillus niger (BAN), biotransformation extract of Aspergillus oryzae (BAO) and BMW, respectively...
2017: PeerJ
https://www.readbyqxmd.com/read/28557429/comparative-proteome-analysis-between-high-lipid-producing-strain-mucor-circinelloides-wj11-and-low-lipid-producing-strain-cbs-277-49
#15
COMPARATIVE STUDY
Xin Tang, Haiqin Chen, Zhennan Gu, Hao Zhang, Yong Q Chen, Yuanda Song, Wei Chen
Mucor circinelloides is one of few oleaginous fungi that produces a useful oil rich in γ-linolenic acid, but it usually only produces <25% total lipid. Nevertheless, we isolated a new strain WJ11 that can produce up to 36% lipid of cell dry weight. In this study, we have systematically analyzed the global changes in protein levels between the high lipid-producing strain WJ11 and the low lipid-producing strain CBS 277.49 (15%, lipid/cell dry weight) at lipid accumulation phase through comparative proteome analysis...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28544612/ecology-adaptation-and-function-of-methane-sulfidic-spring-water-biofilm-microorganisms-including-a-strain-of-anaerobic-fungus-mucor-hiemalis
#16
Enamul Hoque, Johannes Fritscher
Ecological aspects, adaptation, and some functions of a special biofilm and its unique key anaerobic fungus Mucor hiemalis strain EH11 isolated from a pristine spring (Künzing, Bavaria, Germany) are described. The spring's pure nature is characterized by, for example, bubbling methane, marine-salinity, mild hydrothermal (~19.1°C), sulfidic, and reductive-anoxic (Eh : -241 to -253 mV, O2 : ≤ 0.1 mg/L) conditions. It is geoecologically located at the border zone between Bavarian Forest (crystalline rocky mountains) and the moor-like Danube River valley, where geological displacements bring the spring's water from the deeper layers of former marine sources up to the surface...
May 24, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28538103/detoxification-of-fusaric-acid-by-the-soil-microbe-mucor-rouxii
#17
Frankie K Crutcher, Lorraine S Puckhaber, Alois A Bell, Jinggao Liu, Sara E Duke, Robert D Stipanovic, Robert L Nichols
Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin fusaric acid (5-butyl-2-pyridinecarboxylic acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28523604/chemo-enzymatic-synthesis-of-a-glycosylated-peptide-containing-a-complex-n-glycan-based-on-unprotected-oligosaccharides-by-using-dmt-mm-and-endo-m
#18
Yusuke Tomabechi, Toshihiko Katoh, Munetaka Kunishima, Toshiyuki Inazu, Kenji Yamamoto
For chemo-enzymatic synthesis of a glycosylated peptide, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) was used for the synthesis of a N-acetylglucosaminyl peptide and a pseudoglycopeptide by solid-phase peptide synthesis without the requirement of protecting groups on the carbohydrate. We also performed transglycosylation of an N-glycan to the N-acetylglucosaminyl peptide using endo-β-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) to synthesize a glycopeptide containing a complex N-glycan...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28500420/purification-biochemical-and-structural-characterization-of-a-novel-fibrinolytic-enzyme-from-mucor-subtilissimus-ucp-1262
#19
Thiago Pajeú Nascimento, Amanda Emmanuelle Sales, Tatiana Souza Porto, Romero Marcos Pedrosa Brandão Costa, Leonid Breydo, Vladimir N Uversky, Ana Lúcia Figueiredo Porto, Attilio Converti
Fibrinolytic proteases are enzymes that degrade fibrin. They provide a promising alternative to existing drugs for thrombolytic therapy. A protease isolated from the filamentous fungus Mucor subtilissimus UCP 1262 was purified in three steps by ammonium sulfate fractionation, ion exchange, and molecular exclusion chromatographies, and characterized biochemically and structurally. The purified protease exhibited a molecular mass of 20 kDa, an apparent isoelectric point of 4.94 and a secondary structure composed mainly of α-helices...
August 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28484910/responses-of-the-antioxidative-and-biotransformation-enzymes-in-the-aquatic-fungus-mucor-hiemalis-exposed-to-cyanotoxins
#20
Evelyn Balsano, Maranda Esterhuizen-Londt, Enamul Hoque, Stephan Pflugmacher Lima
OBJECTIVES: To investigate antioxidative and biotransformation enzyme responses in Mucor hiemalis towards cyanotoxins considering its use in mycoremediation applications. RESULTS: Catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx) in M. hiemalis maintained their activities at all tested microcystin-LR (MC-LR) exposure concentrations. Cytosolic glutathione S-transferase (GST) activity decreased with exposure to 100 µg MC-LR l(-1) while microsomal GST remained constant...
May 8, 2017: Biotechnology Letters
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