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https://www.readbyqxmd.com/read/28538103/detoxification-of-fusaric-acid-by-the-soil-microbe-mucor-rouxii
#1
Frankie K Crutcher, Lorraine S Puckhaber, Alois A Bell, Jinggao Liu, Sara E Duke, Robert D Stipanovic, Robert L Nichols
Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense) has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin fusaric acid (5-butyl-2-pyridinecarboxylic acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the U. S. Fusaric acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil...
May 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28523604/chemo-enzymatic-synthesis-of-a-glycosylated-peptide-containing-a-complex-n-glycan-based-on-unprotected-oligosaccharides-by-using-dmt-mm-and-endo-m
#2
Yusuke Tomabechi, Toshihiko Katoh, Munetaka Kunishima, Toshiyuki Inazu, Kenji Yamamoto
For chemo-enzymatic synthesis of a glycosylated peptide, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) was used for the synthesis of a N-acetylglucosaminyl peptide and a pseudoglycopeptide by solid-phase peptide synthesis without the requirement of protecting groups on the carbohydrate. We also performed transglycosylation of an N-glycan to the N-acetylglucosaminyl peptide using endo-β-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) to synthesize a glycopeptide containing a complex N-glycan...
May 18, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28500420/purification-biochemical-and-structural-characterization-of-a-novel-fibrinolytic-enzyme-from-mucor-subtilissimus-ucp-1262
#3
Thiago Pajeú Nascimento, Amanda Emmanuelle Sales, Tatiana Souza Porto, Romero Marcos Pedrosa Brandão Costa, Leonid Breydo, Vladimir N Uversky, Ana Lúcia Figueiredo Porto, Attilio Converti
Fibrinolytic proteases are enzymes that degrade fibrin. They provide a promising alternative to existing drugs for thrombolytic therapy. A protease isolated from the filamentous fungus Mucor subtilissimus UCP 1262 was purified in three steps by ammonium sulfate fractionation, ion exchange, and molecular exclusion chromatographies, and characterized biochemically and structurally. The purified protease exhibited a molecular mass of 20 kDa, an apparent isoelectric point of 4.94 and a secondary structure composed mainly of α-helices...
May 12, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28484910/responses-of-the-antioxidative-and-biotransformation-enzymes-in-the-aquatic-fungus-mucor-hiemalis-exposed-to-cyanotoxins
#4
Evelyn Balsano, Maranda Esterhuizen-Londt, Enamul Hoque, Stephan Pflugmacher Lima
OBJECTIVES: To investigate antioxidative and biotransformation enzyme responses in Mucor hiemalis towards cyanotoxins considering its use in mycoremediation applications. RESULTS: Catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx) in M. hiemalis maintained their activities at all tested microcystin-LR (MC-LR) exposure concentrations. Cytosolic glutathione S-transferase (GST) activity decreased with exposure to 100 µg MC-LR l(-1) while microsomal GST remained constant...
May 8, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28476909/construction-of-a-recyclable-genetic-maker-and-serial-gene-deletions-in-the-human-pathogenic-mucorales-mucor-circinelloides
#5
Alexis Garcia, Gloria Adedoyin, Joseph Heitman, Soo Chan Lee
Mucor circinelloides is a human pathogen, biofuel producer, and model system that belongs to a basal fungal lineage; however, the genetics of this fungus are limited. In contrast to ascomycetes and basidiomycetes, basal fungal lineages have been understudied. This may be caused by a lack of attention given to these fungi, as well as limited tools for genetic analysis. Nonetheless, the importance of these fungi as pathogens and model systems has increased. M. circinelloides is one of a few genetically tractable organisms in the basal fungi, but it is far from a robust genetic system when compared to model fungi in the subkingdom dikarya...
May 5, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28455587/comprehensive-analysis-of-fungal-diversity-and-enzyme-activity-in-nuruk-a-korean-fermenting-starter-for-acquiring-useful-fungi
#6
Emily Carroll, Tran Ngoc Trinh, Hokyoung Son, Yin-Won Lee, Jeong-Ah Seo
Nuruk is a fermenting starter that is involved in the production of alcoholic beverages, and has been used in South Korea for a very long time. To analyze the fungal diversity, we collected a total of 59 nuruk samples from several companies and persons in 2013 to 2014, and obtained 364 isolates. All of the single isolated fungi were identified, both morphologically and molecularly, based on the sequences of ribosomal RNA gene [18S, ITS1-5.8S-ITS2, and 26S (D1/D2 region)]. In 46 nuruk samples out of 59 (78%), Saccharomycopsis fibuligera, a dimorphic yeast, was most frequently isolated...
May 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28421050/reveromycins-a-and-b-from-streptomyces-sp-3-10-antifungal-activity-against-plant-pathogenic-fungi-in-vitro-and-in-a-strawberry-food-model-system
#7
Ang Lyu, Hao Liu, Hongjie Che, Long Yang, Jing Zhang, Mingde Wu, Weidong Chen, Guoqing Li
This study was conducted to determine the antifungal activity of the metabolites from Streptomyces sp. 3-10, and to purify and identify the metabolites. Meanwhile, the taxonomic status of strain 3-10 was re-evaluated. The cultural filtrates of strain 3-10 in potato dextrose broth were extracted with ethyl acetate. The resulting crude extract at 1 and 5 μg/ml inhibited growth of 22 species in 18 genera of plant pathogenic fungi and Oomycetes, accounting for 92% of the total 24 tested species, suggesting that it has a wide antifungal spectrum...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28407499/alleviation-of-heavy-metal-toxicity-and-phytostimulation-of-brassica-campestris-l-by-endophytic-mucor-sp-mhr-7
#8
Mahwish Zahoor, Muhammad Irshad, Hazir Rahman, Muhammad Qasim, Sahib Gul Afridi, Muhammad Qadir, Anwar Hussain
Heavy metal (HM) pollution is of great concern in countries like Pakistan where a huge proportion of human population is exposed to it. These toxic metals are making their way from water bodies to soil where it not only interferes with plant growth and development but also initiates serious health issues in human consuming the produce of such soils. Bioremediation is one of the most viable and efficient solution for the problem. Purpose of the current study was to isolate endophytic fungi from plants grown on HM contaminated soil and screen them for their ability to tolerate multiple HM including chromium (Cr(6+)), manganese (Mn(2+)), cobalt (Co(2+)), copper (Cu(2+)) and zinc (Zn(2+))...
August 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28356670/an-unusual-case-of-gastrointestinal-mucormycosis-in-a-patient-with-nephrotic-syndrome
#9
S Sriperumbuduri, K Kalidindi, H Megha, S Guditi, G Taduri
Immunosuppression predisposes to a variety of infections, some of which occur at unusual sites. Of all the forms of mucormycosis, involvement of the intestines is rare. High degree of suspicion along with the use of invasive procedures can facilitate the diagnosis of these infections. We report a rare case of gastrointestinal Mucor in a patient with nephrotic syndrome.
March 2017: Indian Journal of Nephrology
https://www.readbyqxmd.com/read/28339467/a-non-canonical-rna-degradation-pathway-suppresses-rnai-dependent-epimutations-in-the-human-fungal-pathogen-mucor-circinelloides
#10
Silvia Calo, Francisco E Nicolás, Soo Chan Lee, Ana Vila, Maria Cervantes, Santiago Torres-Martinez, Rosa M Ruiz-Vazquez, Maria E Cardenas, Joseph Heitman
Mucorales are a group of basal fungi that includes the casual agents of the human emerging disease mucormycosis. Recent studies revealed that these pathogens activate an RNAi-based pathway to rapidly generate drug-resistant epimutant strains when exposed to stressful compounds such as the antifungal drug FK506. To elucidate the molecular mechanism of this epimutation pathway, we performed a genetic analysis in Mucor circinelloides that revealed an inhibitory role for the non-canonical RdRP-dependent Dicer-independent silencing pathway, which is an RNAi-based mechanism involved in mRNA degradation that was recently identified...
March 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28330225/metagenomics-analysis-of-microbial-communities-associated-with-a-traditional-rice-wine-starter-culture-xaj-pitha-of-assam-india
#11
Sudipta Sankar Bora, Jyotshna Keot, Saurav Das, Kishore Sarma, Madhumita Barooah
This is the first report on the microbial diversity of xaj-pitha, a rice wine fermentation starter culture through a metagenomics approach involving Illumine-based whole genome shotgun (WGS) sequencing method. Metagenomic DNA was extracted from rice wine starter culture concocted by Ahom community of Assam and analyzed using a MiSeq(®) System. A total of 2,78,231 contigs, with an average read length of 640.13 bp, were obtained. Data obtained from the use of several taxonomic profiling tools were compared with previously reported microbial diversity studies through the culture-dependent and culture-independent method...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28316424/ovicidal-effect-of-chitinase-and-protease-enzymes-produced-by-soil-fungi-on-the-camel-tick-hyalomma-dromedarii-eggs-acari-ixodidae
#12
Salwa M Habeeb, Heba M Ashry, Moataza M Saad
The aim of this study was to evaluate the effect of chitinase and protease enzymes produced by environmentally safe soil Fungi; Aspergillus sp. NRC 4/5H; Mucor sp. NRC 5; Trichoderma sp. NRC 4/56; Aspergillus sojae; Mucor ranosisinus; Aspergillus oryzae on embryo development of the camel tick Hyalomma dromedarii eggs. The experiment was carried out on the 7 day aged eggs. Concentrations of the chitinase and protease crude enzymes [1:1, 1:2, 1:3, 1:4 and 1:5 (v/v)] were prepared from six stock solutions whose concentrations were 5 units/ml each...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28303252/evaluation-of-the-biocontrol-potential-of-purpureocillium-lilacinum-qlp12-against-verticillium-dahliae-in-eggplant
#13
Xingjie Lan, Jing Zhang, Zhaofeng Zong, Qing Ma, Yang Wang
A fungus with broad spectrum antifungal activity was isolated from the soil in Qinling Mountain, Shaanxi Province, in China. The fungus was identified as Purpureocillium lilacinum based on ITS rDNA gene analysis. The strain, coded as QLP12, showed high inhibition activity on fungal mycelium growth in vitro, especially to Mucor piriformis, Trichothecium roseum, Rhizoctonia solani, and Verticillium dahliae, and its potential for biocontrol efficacy of eggplant. Verticillium wilt disease caused by Verticillium dahliae among 10 fungal species tested was explored...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28276198/anthropogenic-impact-on-environmental-filamentous-fungi-communities-along-the-mediterranean-littoral
#14
Mohammed Hashim Al-Yasiri, Anne-Cécile Normand, Jean-François Mauffrey, Stéphane Ranque
We hypothesised that anthropogenic influences impact the filamentous fungi community structure and that particular species or species patterns might serve as markers to characterise ecosystems. This study aimed to describe the filamentous fungi community structure in various biotopes along the Mediterranean shore that were exposed to various levels of anthropogenic influence. We sampled filamentous fungi from yellow-legged gull faecal samples at five study sites along the Mediterranean littoral in southern France...
March 9, 2017: Mycoses
https://www.readbyqxmd.com/read/28275659/dataset-of-differentially-accumulated-proteins-in-mucor-strains-representative-of-four-species-grown-on-synthetic-potato-dextrose-agar-medium-and-a-cheese-mimicking-medium
#15
Stéphanie Morin-Sardin, Jean-Luc Jany, Sébastien Artigaud, Vianney Pichereau, Benoît Bernay, Emmanuel Coton, Stéphanie Madec
The data presented are associated with the "Proteomic analysis of the adaptative response of Mucor spp. to cheese environment" (Morin-Sardin et al., 2016) article [1]. Mucor metabolism is poorly documented in the literature and while morphology and growth behavior suggest potential adaptation to cheese for some strains, no adaptation markers to cheese environment have been identified for this genus. To establish the possible existence of metabolic functions related to cheese adaptation, we used a gel based 2-DE proteomic approach coupled to LC-MS/MS to analyze three strains from species known or proposed to have a positive or negative role in cheese production as well as a strain from a non-related cheese-species...
April 2017: Data in Brief
https://www.readbyqxmd.com/read/28251531/enhanced-bioremediation-of-lead-contaminated-soil-by-solanum-nigrum-l-with-mucor-circinelloides
#16
Liqun Sun, Xiufeng Cao, Min Li, Xu Zhang, Xinxin Li, Zhaojie Cui
Strain selected from mine tailings in Anshan for Pb bioremediation was characterized at the genetic level by internal transcribed spacer (ITS) sequencing. Results revealed that the strain belongs to Mucor circinelloides. Bioremediation of lead-contaminated soil was conducted using Solanum nigrum L. combined with M. circinelloides. The removal efficacy was in the order microbial/phytoremediation > phytoremediation > microbial remediation > control. The bioremediation rates were 58.6, 47...
March 1, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28242552/enzymatic-and-solid-phase-synthesis-of-new-donepezil-based-l-and-d-glutamic-acid-derivatives-and-their-pharmacological-evaluation-in-models-related-to-alzheimer-s-disease-and-cerebral-ischemia
#17
Leticia Monjas, Mariana P Arce, Rafael León, Javier Egea, Concepción Pérez, Mercedes Villarroya, Manuela G López, Carmen Gil, Santiago Conde, María Isabel Rodríguez-Franco
Previously, we have described N-Bz-L-Glu[NH-2-(1-benzylpiperidin-4-yl)ethyl]-O-nHex (IQM9.21, L-1) as an interesting multifunctional neuroprotective compound for the potential treatment of neurodegenerative diseases. Here, we describe new derivatives and different synthetic routes, such as chemoenzymatic and solid-phase synthesis, aiming to improve the previously described route in solution. The lipase-catalysed amidation of L- and D-Glu diesters with N-benzyl-4-(2-aminoethyl)piperidine has been studied, using Candida antarctica and Mucor miehei lipases...
April 21, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28182334/utility-of-intraoperative-frozen-sections-in-surgical-decision-making-for-acute-invasive-fungal-rhinosinusitis
#18
Peter Papagiannopoulos, Diana Murro Lin, Samer Al-Khudari, Kumar Rajan, Swathi Reddy, Paulo Gattuso, Bobby Tajudeen, Pete S Batra
BACKGROUND: Acute invasive fungal rhinosinusitis (AIFRS) represents a fulminant, potentially fatal, disease process in immunocompromised patients. The diagnosis often rests on high index of clinical suspicion, with relative paucity of data on the diagnostic and therapeutic implications of intraoperative frozen sections. METHODS: Retrospective review was performed for 18 cases undergoing endoscopic sinus surgery for AIFRS. Reliability of intraoperative frozen section diagnosis was evaluated for all patients using final pathology as the gold standard...
May 2017: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/28165868/effect-of-the-filamentous-fungus-mucor-circinelloides-on-the-development-of-eggs-of-the-rumen-fluke-calicophoron-daubneyi-paramphistomidae
#19
Fabià  N Arroyo, Josà  à  Ngel Hernà  Ndez, Cristiana Filipa Cazapal-Monteiro, Josà  Pedreira, Jaime Sanchà  S, à  Ngel Romasanta, Rita Sã  Nchez-Andrade, Adolfo Paz-Silva, Marà  A Sol Arias
Ruminants infected by Paramphistomidae flukes shed eggs in the feces, which pass through different stages in the environment until the infective stages (metacercariae) are reached. The activity of the soil fungus Mucor circinelloides on the development of eggs of the rumen fluke Calicophoron daubneyi was presently tested with 3 probes, i.e., in Petri plates, feces and an aqueous environment (tubes). The effect of the fungus was assessed by recording the numbers of undeveloped, non-viable and embryonated eggs...
February 6, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28154489/characterization-of-two-new-records-of-mucoralean-species-isolated-from-gut-of-soldier-fly-larva-in-korea
#20
Thi Thuong Thuong Nguyen, Tham Thi Duong, Hyang Burm Lee
While surveying the diversity of fungi of the order Mucorales, two isolates, EML-PUKI12-1 and EML-PUKI06-1, were obtained from the gut of soldier fly larvae inhabiting the bulrush at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. The isolates were confirmed as Mucor irregularis and Mucor fragilis species, respectively, based on the morphological characteristics and phylogenetic analysis of rDNA internal transcribed spacer region. Such mucoralean species belonging to undiscovered taxa has not previously been described in Korea...
December 2016: Mycobiology
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