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https://www.readbyqxmd.com/read/28899635/fast-technique-for-the-identification-of-gilbertella-persicaria-via-optical-microscopy
#1
Isabel Cruz-Lachica, Isidro Marquez-Zequera, Raul Allende-Molar, Josefa Adriana Sañudo-Barajas, Josefina Leon-Felix, Raymundo Saul Garcia-Estrada
Gilbertella persicaria is an important phytopathogen that is confused with Mucor spp. and Rhizopus spp. The main distinguishing characteristic of G. persicaria is the presence of appendages in sporangiospores, and their observation by conventional staining techniques generally fails. A technique is described using light microscopy for fast and reliable diagnosis.
September 9, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28878325/the-influence-of-the-mating-type-on-virulence-of-mucor-irregularis
#2
Wenqi Xu, Guanzhao Liang, Jingwen Peng, Zhimin Long, Dongmei Li, Meihua Fu, Qiong Wang, Yongnian Shen, Guixia Lv, Huan Mei, Clement K M Tsui, Weida Liu
Mucor irregularis is an emerging fungal pathogen that cause cutaneous infection and could cause death. However, little is known about its mechanism of pathogenesis. There is evidence suggesting virulence vary with mating types in fungi, including the Mucorales. Here, we characterized the mating type locus of M. irregularis and the mating type ratio of 17 clinical isolates in China. Genomic data indicated M. irregularis is heterothallic having two mating types - bearing either SexP or SexM allele. Also, we employed a mice model to study the inflammation and pathological effects of different mating types...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28867698/meta-analysis-on-the-influence-of-antifungal-spectrum-on-effectiveness-of-empirical-antifungal-therapy-for-febrile-neutropenia
#3
Jinshi Irikuchi, Toru Imai, Masayo Tanaka, Mitiya Tanuma, Takao Orii, Toshiaki Kato
 We conducted a meta-analysis to investigate the influence of antifungal spectrum on the effectiveness and adverse events of empirical antifungal therapy for febrile neutropenia. We searched PubMed and Cochrane Central Register of Controlled Trials (Central), and identified randomized controlled trials reporting mortality, efficacy, adverse events, and hepatic and renal dysfunction. Five trials assessed the efficacy and adverse events of agents with antifungal spectrum covering and those not covering Aspergillus...
2017: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/28859156/longterm-storage-of-post-packaged-bread-by-controlling-spoilage-pathogens-using-lactobacillus-fermentum-c14-isolated-from-homemade-curd
#4
Soma Barman, Ranjan Ghosh, Shreya Sengupta, Narayan C Mandal
One potent lactic acid bacterial strain C14 with strong antifungal activity was isolated from homemade curd. Based on morphological as well as biochemical characters and 16S rDNA sequence homology the strain was identified as Lactobacillus fermentum. It displayed a wide antimicrobial spectrum against both Gram-positive and Gram-negative pathogenic bacteria, and also against number of food spoilage, plant and human pathogenic fungi. The cell free supernatant (CFS) of the strain C14 was also effective against the fungi tested...
2017: PloS One
https://www.readbyqxmd.com/read/28857682/assessing-the-potential-of-fatty-acids-produced-by-filamentous-fungi-as-feedstock-for-biodiesel-production
#5
Juan Daniel Rivaldi, Ana Karine F Carvalho, Leyvison Rafael V da Conceição, Heizir F de Castro
Increased costs and limited availability of traditional lipid sources for biodiesel production encourages researchers to find more sustainable feedstock at low prices. Microbial lipid stands out as feedstock replacement for vegetable oil to convert fatty acid esters (FAEs). In this study, the potential of three isolates of filamentous fungi (Mucor circinelloides URM 4140, M. hiemalis URM 4144 and Penicillium citrinum URM 4126) has been assessed as single-cell oil (SCO) producers. M. circinelloides 4140 had the highest biomass concentration with lipid accumulation of up to 28 wt% at 120 h of cultivation...
August 31, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28854485/erythritol-production-by-yarrowia-lipolytica-from-okara-pretreated-with-the-in-house-enzyme-pools-of-fungi
#6
Xiaoyan Liu, Xinjun Yu, Jun Xia, Jinshun Lv, Jiaxing Xu, Benlin Dai, Xiangqian Xu, Jiming Xu
Okara (soybean residue) is an ideal erythritol feedstock due to its low price and high-nutrient content. Fungal-fermentations were carried out in okara using in-house enzyme pools generated by Mucor flavus or Trichoderma reesei to make okara more accessibility in the subsequent erythritol production using Yarrowia lipolytica. Mucor-fermented okara produced a high erythritol yield because of its special component and micromorphology. Five days is the optimal period for Mucor fermentation. Different fermentation modes were compared in terms of erythritol production and yield...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28826260/microbial-transformation-of-quercetin-and-its-prenylated-derivatives
#7
Yina Xiao, Ik-Soo Lee
The microbial transformation studies of 7-O-prenylquercetin (1), 4'-O-prenylquercetin (2) and quercetin (3) were investigated with 20 different microbial strains to discover new metabolites. It was revealed that the fungus Mucor hiemalis was the most appropriate micro-organism which was capable of transforming these flavonoids. Structures of the three new (4-6) and one known (7) metabolites were elucidated as 7-O-prenylquercetin 3-O-β-D-glucopyranoside (4), 4'-O-prenylquercetin 3-O-β-D-glucopyranoside (5), 4'-O-prenylquercetin 3'-O-β-D-glucopyranoside (6) and quercetin 5-O-β-D-glucopyranoside (7) by the spectroscopic methods...
August 22, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28816606/scale-up-of-puf-immobilized-fungal-chitosanase-lipase-preparation-production
#8
Katarzyna Struszczyk-Świta, Łukasz Stańczyk, Mirosława Szczęsna-Antczak, Tadeusz Antczak
Mucor circinelloides IBT-83 mycelium that exhibits both lipolytic (AL) and chitosanolytic (ACH) activities was immobilized into polyurethane foam (PUF) in a 30 L laboratory fermenter. The process of immobilization was investigated in terms of: the carrier porosity, its type, amount and shape, location inside the fermenter, mixing and aeration parameters during the culture, as well as downstream processing operations. The selected conditions allowed for immobilization of approx. 7 g of defatted and dried mycelium in 1 g of carrier, i...
August 17, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28800827/modelling-the-effect-of-water-activity-reduction-by-sodium-chloride-or-glycerol-on-conidial-germination-and-radial-growth-of-filamentous-fungi-encountered-in-dairy-foods
#9
Nicolas Nguyen Van Long, Karim Rigalma, Louis Coroller, Robin Dadure, Stella Debaets, Jérôme Mounier, Valérie Vasseur
Water activity (aw) is one of the most influential abiotic factors affecting fungal development in foods. The effects of aw reduction on conidial germination and radial growth are generally studied by supplementing culture medium with the non-ionic solute glycerol despite food aw can also depend on the concentration of ionic solutes such as sodium chloride (NaCl). The present study aimed at modelling and comparing the effects of aw, either modified using NaCl or glycerol, on radial growth and/or conidial germination parameters for five fungal species occurring in the dairy environment...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28767050/filamentous-fungal-human-pathogens-from-food-emphasising-aspergillus-fusarium-and-mucor
#10
REVIEW
R Russell M Paterson, Nelson Lima
Disease caused by filamentous fungal human pathogens (FFHP) is increasing. These organisms cause severe mycoses in immunosuppressed individuals, such as those: (a) with AIDS; (b) having undergone transplantation; and/or (c) undergoing chemotherapy. Immunocompetent people can become infected. Some FFHP are isolated from foods which may be fomites. However, the information concerning particular species on specific food is large, dispersed and difficult to obtain. Reports of filamentous fungi from food/crops and causing human disease are frequently only available in the literature of food mycology/plant pathology and medical mycology, respectively: it is seldom cross-referenced...
August 2, 2017: Microorganisms
https://www.readbyqxmd.com/read/28764159/otomycosis-in-a-rural-community-attending-a-tertiary-care-hospital-assessment-of-risk-factors-and-identification-of-fungal-and-bacterial-agents
#11
Priti Agarwal, Leimapokpam Sumitra Devi
INTRODUCTION: Otomycosis is one of the frequently encountered fungal infections of external auditory canal, commonly seen in tropical and subtropical regions of the world. Various host (local, systemic) and environmental factors can predispose a person to otomycosis. Although clinical presentations along with otoscopic findings of the patients are well suggestive of fungal infection, proper identification of causative agents is mandatory in order to prevent recurrences and complications...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28757006/-pulmonary-mucormycosis-in-a-patient-with-kidney-transplant-and-uncontrolled-haemoptysis
#12
Dulce Iliana Navarro Vergara, Gloria Barragán Pola, Alexandro Bonifaz, Carlos Núñez Pérez-Redondo, Omar Choreño García, Raúl Cicero Sabido
BACKGROUND: Pulmonary mucormycosis is a rare opportunistic infection with high mortality that is caused by species of Mucorales. The most common species involved are Rhizopus, Mucor, Lichtheimia, and Rhizomucor. CASE REPORT: A 56 year-old woman presented with a clinical history of diabetes mellitus type 2 and chronic renal disease. She underwent a cadaveric kidney transplantation two years before her admission, for which immunomodulating therapy with thymoglobulin, tacrolimus, mofetil-microphenolate and prednisone was established...
July 27, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28751211/microbial-f-type-lectin-domains-with-affinity-for-blood-group-antigens
#13
Sonal Mahajan, Aasawari Khairnar, Ritika Bishnoi, T N C Ramya
F-type lectins are fucose binding lectins with characteristic fucose binding and calcium binding motifs. Although they occur with a selective distribution in viruses, prokaryotes and eukaryotes, most biochemical studies have focused on vertebrate F-type lectins. Recently, using sensitive bioinformatics search techniques on the non-redundant database, we had identified many microbial F-type lectin domains with diverse domain organizations. We report here the biochemical characterization of F-type lectin domains from Cyanobium sp...
September 23, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28726315/biomineralization-of-gold-by-mucor-plumbeus-the-progress-in-understanding-the-mechanism-of-nanoparticles-formation
#14
Irena Maliszewska, Włodzimierz Tylus, Izabela Pawlaczyk-Graja, Wojciech Pusz, Anna Baturo-Cieśniewska, Jacek Chęcmanowski, Bogdan Szczygieł
This contribution describes the deposition of gold nanoparticles by microbial reduction of Au(III) ions using the mycelium of Mucor plumbeus. Biosorption as the major mechanism of Au(III) ions binding by the fungal cells and the reduction of them to the form of Au(0) on/in the cell wall, followed by the transportation of the synthesized gold nanoparticles to the cytoplasm, is postulated. The probable mechanism behind the reduction of Au(III) ions is discussed, leading to the conclusion that this process is non enzymatic one...
July 20, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28718996/production-of-ligninolytic-enzymes-and-some-diffusible-antifungal-compounds-by-white-rot-fungi-using-potato-solid-wastes-as-the-sole-nutrient-source
#15
H Schalchli, E Hormazábal, O Rubilar, G Briceño, A Mutis, G J Zocolo, M C Diez
AIMS: The aim of this study was to evaluate the synthesis of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi (WRF) using peels or discarded potato as the sole nutrient source. METHODS AND RESULTS: The strain Trametes hirsuta Ru-513 highlighted for its laccase activity (595 ± 33 U l(-1) ), which is able to decolourize 87% of an anthraquinone dye using potato peels as the sole nutritional support. A native polyacrylamide gel of laccase proteins showed the presence of two isoenzymes, corresponding to proteins of 56 and 67 kDa, which were detected by SDS-PAGE...
July 18, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28698667/phylogenetic-analysis-reveals-two-genotypes-of-the-emerging-fungus-mucor-indicus-an-opportunistic-human-pathogen-in-immunocompromised-patients
#16
Saad J Taj-Aldeen, Muna Almaslamani, Bart Theelen, Teun Boekhout
Mucormycosis is a rare fungal infection caused by Mucor indicus. Phylogenetic analysis of many M. indicus isolates, mainly sampled from different clinical and environmental specimens collected worldwide, revealed two genotypes, I and II, based on ITS and D1/D2 LSU rDNA sequences. A retrospective review of the literature revealed 13 cases. Eight (76.9%) patients had disseminated infections, and the overall mortality rate was 30.7%. A pulmonary infection caused by M. indicus genotype I in a liver transplant recipient was disseminated to include the skin and was successfully treated with liposomal amphotericin B and aggressive surgery...
July 12, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28687045/sageretia-thea-osbeck-modulated-biosynthesis-of-nio-nanoparticles-and-their-in-vitro-pharmacognostic-antioxidant-and-cytotoxic-potential
#17
Ali Talha Khalil, Muhammad Ovais, Ikram Ullah, Muhammad Ali, Zabta Khan Shinwari, Dilawar Hassan, Malik Maaza
NiO nanoparticles are biosynthesized using Sageretia thea (Osbeck.) aqueous leave extracts and their biological activities are reported. Nanoparticles (∼18 nm) were characterized through XRD, ATR-FTIR, EDS, SAED, HR-SEM/TEM and Raman spectroscopy. Antibacterial activity was investigated against six pathogenic bacterial strains (gram positive and gram negative) and their corresponding minimum inhibitory concentrations (MICs) were calculated. UV-exposed nanoparticles were investigated to have reduced MICs relative to the NiO nanoparticles have not been exposed to UV...
July 7, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28681286/bioremoval-of-arsenic-v-from-aqueous-solutions-by-chemically-modified-fungal-biomass
#18
J F Cárdenas-González, I Acosta-Rodríguez, Y Téran-Figueroa, A S Rodríguez-Pérez
The biosorption of arsenic (V) on nine chemically modified biomasses (with iron oxide coated) of mycelia fungi: Aspergillus flavus III, IV and V, Aspergillus fumigatus I-II, Paecilomyces sp., Cladosporium sp., Mucor sp-1 and 2 was studied in this work. This study provides evidence that the biomasses of the fungi A. flavus, IV, III and V, Paecilomyces sp., and A. fumigatus I were very efficient at removing 1 mg/L of the metal in solution, using atomic absorption spectroscopy (AAS), achieving the following percentage of removals: 97...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28667347/clinical-analysis-of-diabetic-combined-pulmonary-mucormycosis
#19
Yu-Hong Li, Peng Sun, Jian-Chao Guo
OBJECTIVE: To improve the understanding of diabetic combined pulmonary mucormycosis. METHODS AND RESULTS: The clinical data of twelve patients diagnosed as diabetic combined pulmonary mucormycosis were analyzed by the clinical manifestations, imaging features, diagnosis, treatment and prognosis of this disease. Patients from January 1999 to June 2015 in our hospital had poor blood glucose control and varying degrees of diabetic chronic complications with an on average of diabetes 8...
June 30, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28659561/detection-of-fungi-from-an-indoor-environment-using-loop-mediated-isothermal-amplification-lamp-method
#20
Takako Nakayama, Takashi Yamazaki, Ayaka Yo, Kazuya Tone, Mohamed Mahdi Alshahni, Ryuichi Fujisaki, Koichi Makimura
 Loop-mediated isothermal amplification (LAMP) is a useful DNA detection method with high specificity and sensitivity. The LAMP reaction is carried out within a short time at a constant temperature without the need for thermal cycling. We developed a LAMP primer set for detecting a wide range of fungi by aligning the sequences of the large subunit ribosomal RNA gene of Candida albicans (Ascomycota), Cryptococcus neoformans (Basidiomycota), and Mucor racemosus (Mucorales). The threshold of C. albicans rDNA as template with our LAMP primer set was in the range of 10-100 copies per a reaction...
2017: Biocontrol Science
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