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J Denis, M-P Ledoux, Y Nivoix, R Herbrecht
Triazoles compounds are first-line agents for the treatment of invasive fungal diseases. Isavuconazole is the most recent triazole compound, approved in 2015 by the FDA and the EMA to treat invasive aspergillosis and mucormycosis. We reviewed here the in vitro activity of isavuconazole against a vast spectrum of species. Isavuconazole MICs were evaluated using CLSI, EUCAST or Etest methods, with no significant differences between the technics. Low MIC50 and MIC90 (<1μg/mL) were described for isavuconazole against the majority of Candida spp...
March 10, 2018: Journal de Mycologie Médicale
Rhiannon L Wallace, Danielle L Hirkala, Louise M Nelson
The ability of <i>Pseudomonas fluorescens</i> isolates 1-112, 2-28, and 4-6, to control <i>Mucor piriformis</i> (Mucor rot) on 'Gala', 'McIntosh', 'Ambrosia', and 'Spartan' apples in commercial cold storage, and their possible mechanisms of action were investigated. Isolates 1-112 and 2-28, provided significant levels of disease control on 'McIntosh' and 'Spartan' apples, while isolate 4-6 provided control of Mucor rot on 'Gala' and 'Spartan' apples compared with control fruits after 15 weeks storage at 0°C...
March 5, 2018: Canadian Journal of Microbiology
Anna Kowalska, Tomasz Boruta, Marcin Bizukojć
The application of microparticle-enhanced cultivation (MPEC) is an attractive method to control mycelial morphology, and thus enhance the production of metabolites and enzymes in the submerged cultivations of filamentous fungi. Unfortunately, most literature data deals with the spore-agglomerating species like aspergilli. Therefore, the detailed quantitative study of the morphological evolution of four different fungal species (Aspergillus terreus, Penicillium rubens, Chaetomium globosum, and Mucor racemosus) based on the digital analysis of microscopic images was presented in this paper...
March 5, 2018: MicrobiologyOpen
Jun-Jin Deng, Wei-Qian Huang, Zhi-Wei Li, De-Lin Lu, Yuanyuan Zhang, Xiao-Chun Luo
The use of cell wall degrading enzymes of Trichoderma is a promising alternative for improving food storage. The aspartic protease P6281 secreted by the fungus Trichoderma harzianum plays an important role in mycoparasitism on phytopathogenic fungi. In this study, recombinant P6281 (rP6281) expressed in Pichia pastoris showed high activity of 321.8 U/mL. Maximum activity was observed at pH 2.5 and 40 °C, and the enzyme was stable in the pH range of 2.5-6.0. rP6281 significantly inhibited spore germination and growth of plant and animal pathogenic fungi such as Botrytis cinerea, Mucor circinelloides, Aspergillus fumigatus, Aspergillus flavus, Rhizoctonia solani, and Candida albicans...
May 2018: Enzyme and Microbial Technology
Xinyi Zan, Xin Tang, Linfang Chu, Yuanda Song
Although the multiple roles of lipases have been reported in yeasts and microalgae, the functions of lipases have not been studied in oleaginous filamentous fungi. Lipase Lip6 has been reported in the oleaginous filamentous fungus Mucor circinelloides with the consensus lipase motif GXSXG and the typical acyltransferase motif of H-(X)4-D. To demonstrate that Lip6 might play dual roles as a lipase and an acyltransferase, we performed site-directed mutagenesis in the lipase motif and the acyltransferase motif of Lip6...
February 28, 2018: Journal of Agricultural and Food Chemistry
Wencheng Song, Xiangxue Wang, Zhongshan Chen, Guodong Sheng, Tasawar Hayat, Xiangke Wang, Yubing Sun
The combined pollution of radionuclides and heavy metals has been given rise to widespread concern during uranium mining. The influence of As(V) on U(VI) immobilization by Mucor circinelloides (M. circinelloides) was investigated using batch experiments. The activity of antioxidative enzymes and concentrations of thiol compounds and organic acid in M. circinelloides increased to respond to different U(VI) and As(V) stress. The morphological structure of M. circinelloides changed obviously under U(VI) and As(V) stress by SEM and TEM analysis...
February 24, 2018: Environmental Pollution
Mitsuhiro Iwamoto, Yukiko Sekiguchi, Kensuke Nakamura, Yoshirou Kawaguchi, Takeshi Honda, Jun Hasegawa
The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties of antibodies. By remodeling N-glycan to obtain a homogeneous glycoform or chemically modified glycan, antibody characteristics can be controlled or modified. Such remodeling can be achieved by transglycosylation reactions using a mutant of endoglycosidase from Streptococcus pyogenes (Endo-S) and glycan oxazoline. In this study, we generated improved mutants of Endo-S by introducing additional mutations to the D233Q mutant...
2018: PloS One
Yu-Ju Chen, Chao-Liang Chou, Kuan-Ju Lai, Ya-Ju Lin Lin
PURPOSE: Invasive mycosis caused by the Aspergillus, Fusarium, and Mucor can be fetal, especially in the immunocompromised patients with central nervous system (CNS) involvement. Here we present a case of CNS Fusarium infection, and this is the first reported case of Fusarium brain abscess in Taiwan. CASE REPORT: A 65-year-old woman presented with fever and conscious disturbance for 3 days. Neurological examination showed stupor consciousness, neck stiffness, multiple cranial nerves palsy, and bilateral Babinski signs...
September 15, 2017: Acta Neurologica Taiwanica
M E Zvezdanova, P Escribano, A Ruiz, M C Martínez-Jiménez, T Peláez, A Collazos, J Guinea, E Bouza, B Rodríguez-Sánchez
In this study we evaluated the capacity of MALDI-TOF MS (Bruker Daltonics, Bremen, Germany) to identify clinical mould isolates. We focused on two aspects of MALDI-TOF MS identification: the sample processing and the database. Direct smearing of the sample was compared with a simplified processing consisting of mechanical lysis of the moulds followed by a protein extraction step. Both methods were applied to all isolates and the Filamentous Fungi Library 1.0 (Bruker Daltonics) was used for their identification...
February 9, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Loida López-Fernández, Marta Sanchis, Patricia Navarro-Rodríguez, Francisco E Nicolás, Fátima Silva-Franco, Josep Guarro, Victoriano Garre, María Isabel Navarro-Mendoza, Carlos Pérez-Arques, Javier Capilla
The increasing number of infections by species of Mucorales and their high mortality constitute an important concern for public health. This study aims to decipher the genetic basis of Mucor circinelloides pathogenicity, which displays virulence in a strain dependent manner. Assuming that genetic differences between strains may be linked to different pathotypes, we have conducted a study to explore genes responsible for virulence in M. circinelloides by whole genome sequencing of the avirulent strain NRRL3631 and comparison with the virulent strain CBS277...
February 13, 2018: Virulence
Wenqi Xu, Jingwen Peng, Dongmei Li, Clement K M Tsui, Zhimin Long, Qiong Wang, Huan Mei, Weida Liu
Mucormycosis is one of the most invasive mycosis and has caused global concern in public health. Cutaneous mucormycosis caused by Mucor irregularis (formerly Rhizomucor variabilis) is an emerging disease in China. To survive in the human body, M. irregularis must overcome the hypoxic (low oxygen) host microenvironment. However, the exact molecular mechanism of its pathogenicity and adaptation to low oxygen stress environment is relatively unexplored. In this study, we used Illumina HiSeq technology (RNA-Seq) to determine and compare the transcriptome profile of M...
October 9, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Abul Hossain Molla, Haider Iqbal Khan
Globally, scarcity of contaminant free water usages is increasing gradually; it might be solved after generation of any sustainable technology to detoxify contaminated waters. An attempt was undertaken to detoxify textile effluent with fungal strains Trichoderma harzianum and Mucor hiemalis. Fungal detoxified effluent and its performance on three crops (wheat, mungbean, and mustard) seed germination in petri dishes and seedlings establishment of mustard in polythene bag were evaluated. Fungal strains significantly detoxified textile effluent by removal of 76% total solids, 91...
February 3, 2018: Environmental Science and Pollution Research International
Nii Korley Kortei, George Tawia Odamtten, Mary Obodai, Michael Wiafe-Kwagyan
The presence of fungi in our foods poses serious health risks as some genera of fungi may produce certain mycotoxins which have carcinogenic, mutagenic, teratogenic, and immunosuppressive effect on humans and animals alike. Fruitbodies of Pleurotus ostreatus were solar dried at a moisture content of 12.5 ± 0.2% and stored in polythene and polypropylene packs, gamma irradiated at doses of 0 (control), 1, 2, 3, 4, and 5 kGy at a dose rate of 1.7 kGy/hr from a Cobalt 60 source (SLL, 515, Hungary) and stored at room temperature 28-30°C for a period of 12 months...
January 2018: Food Science & Nutrition
Madan Kumar, Barbara Jantausch, Clarivet Torres, Joseph Campos, Adrian Zelazny
We report here the isolation of Mucor velutinosus from multiple blood cultures performed on samples from Broviac catheters and culture of a Broviac insertion-site wound sample from a 6-year-old boy with a history of intestinal failure secondary to chronic intestinal pseudo-obstruction, parenteral nutrition, and jejunostomy tube dependence. Examination of a slide from the culture revealed the presence of wide nonseptate hyphae with sporangiophores, columella, and chlamydospores. The fungal isolate was sent to the National Institutes of Health for further evaluation and was identified as Mucor velutinosus by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and genomic sequencing...
January 24, 2018: Journal of the Pediatric Infectious Diseases Society
Thuong T T Nguyen, Hee-Young Jung, Youn Su Lee, Kerstin Voigt, Hyang Burm Lee
During a survey of fungal diversity of the order Mucorales, three zygomycete isolates, CNUFC-YR113-1, CNUFC-KNU16-7, and CNUFC-BS1-1 were isolated from freshwater and soil samples in Korea. The strains were analyzed both morphologically and phylogenetically based on internal transcribed spacer and 28S rDNA gene sequences. Based on their morphology and phylogeny, the CNUFC-YR113-1 and CNUFC-KNU16-7 isolates were identified as Actinomucor elegans, and CNUFC-BS1-1 was identified as Mucor minutus. To the best of our knowledge, the species A...
December 2017: Mycobiology
José A Hernández, Rosa A Vázquez-Ruiz, Cristiana F Cazapal-Monteiro, Esther Valderrábano, Fabián L Arroyo, Iván Francisco, Silvia Miguélez, Rita Sánchez-Andrade, Adolfo Paz-Silva, María S Arias
Abstract: There are certain saprophytic fungi in the soil able to develop an antagonistic effect against eggs of parasites. Some of these fungal species are ingested by animals during grazing, and survive in their feces after passing through the digestive tract. To identify and isolate ovicidal fungi in the feces of wild captive animals, a total of 60 fecal samples were taken from different wild animals kept captive in the Marcelle Natureza Zoological Park (Lugo, Spain). After the serial culture of the feces onto Petri dishes with different media, their parasicitide activity was assayed against eggs of trematodes (Calicophoron daubneyi) and ascarids (Parascaris equorum)...
June 2, 2017: Journal of Fungi (Basel, Switzerland)
Francis M Borges, Thaís O de Paula, Marjorie R A Sarmiento, Maycon G de Oliveira, Maria L M Pereira, Isabela V Toledo, Thiago C Nascimento, Alessandra B Ferreira-Machado, Vânia L Silva, Cláudio G Diniz
Fungi have a complex role in the intestinal tract, influencing health and disease, with dysbiosis contributing to obesity. Our objectives were to investigate fungal diversity in human gut microbiota among eutrophic, overweight, and obese. Epidemiological and nutritional information were collected from adult individuals, as well as stool samples processed for selective fungi isolation and identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (yeasts) or microculture (filamentous fungi)...
January 24, 2018: Current Microbiology
Rafał Ważny, Piotr Rozpądek, Roman J Jędrzejczyk, Marta Śliwa, Anna Stojakowska, Teresa Anielska, Katarzyna Turnau
Phytoremediation of polluted sites can be improved by co-inoculation with mycorrhizal and endophytic fungi. In this study, the effects of single- and co-inoculation of Lactuca serriola with an arbuscular mycorrhizal (AM) fungus, Rhizoglomus intraradices, and endophytic fungi, Mucor sp. or Trichoderma asperellum, on plant growth, vitality, toxic metal accumulation, sesquiterpene lactone production and flavonoid concentration in the presence of toxic metals were evaluated. Inoculation with the AM fungus increased biomass yield of the plants grown on non-polluted and polluted substrate...
January 23, 2018: Mycorrhiza
Purva Bavikar, Varshil Mehta
Mucormycosis is a progressively invasive disease, with a fatal outcome, on late presentation. A 38-year-old female presented with diabetic ketoacidosis with right eye ptosis and a frozen globe without any signs of inflammation, externally. She underwent transnasal endoscopic debridement of paranasal sinuses and exenteration of the right eye. The histopathology specimen revealed the growth of mucormycosis. She was treated with intravenous (IV) amphotericin B, IV insulin, and extensive debridement surgery, but had an unfavorable outcome due to rapid mucor invasion to the brain...
November 13, 2017: Curēus
Syeda Neelam Afroze, Rajani Korlepara, Guttikonda Venkateswara Rao, Jayakiran Madala
Mucormycosis is one of the most rapidly progressing and fulminant forms of fungal infection which usually begins in the nose and paranasal sinuses following inhalation of fungal spores. It is caused by organisms of the subphylum Mucormycotina, including genera as Absidia, Mucor, Rhizomucor, and Rhizopus. The incidence of mucormycosis is approximately 1.7 cases per 1,000,000 inhabitants per year. Mucormycosis affecting the maxilla is rare because of rich blood vessel supply of maxillofacial areas although more virulent fungi such as Mucor can overcome this difficulty...
October 2017: Contemporary Clinical Dentistry
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