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https://www.readbyqxmd.com/read/29240696/metalaxyl-degradation-by-mucorales-strains-gongronella-sp-and-rhizopus-oryzae
#1
Maria Rosário Martins, Cledir Santos, Pablo Pereira, Júlio Cruz-Morais, Nelson Lima
In this study, the degradation of metalaxyl was investigated in the presence of two Mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. Fungal strains were characterised by a polyphasic approach using phylogenetic analysis of the Internal Transcribed Spacer (ITS) gene region, phenotypic characterisation by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) spectral analysis, and growth kinetics experiments...
December 14, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29225797/seasonal-clustering-of-sinopulmonary-mucormycosis-in-patients-with-hematologic-malignancies-at-a-large-comprehensive-cancer-center
#2
Shobini Sivagnanam, Dhruba J Sengupta, Daniel Hoogestraat, Rupali Jain, Zach Stednick, David N Fredricks, Paul Hendrie, Estella Whimbey, Sara T Podczervinski, Elizabeth M Krantz, Jeffrey S Duchin, Steven A Pergam
Background: Invasive Mucorales infections (IMI) lead to significant morbidity and mortality in immunocompromised hosts. The role of season and climatic conditions in case clustering of IMI remain poorly understood. Methods: Following detection of a cluster of sinopulmonary IMIs in patients with hematologic malignancies, we reviewed center-based medical records of all patients with IMIs and other invasive fungal infections (IFIs) between January of 2012 and August of 2015 to assess for case clustering in relation to seasonality...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29217228/primary-cutaneous-mucormycosis-due-to-rhizopus-arrhizus-in-an-8-year-old-girl
#3
M F Albízuri-Prado, A Sánchez-Orta, A Rodríguez-Bandera, M Feito-Rodríguez
No abstract text is available yet for this article.
December 4, 2017: Actas Dermo-sifiliográficas
https://www.readbyqxmd.com/read/29205274/production-of-cellulosic-organic-acids-via-synthetic-fungal-consortia
#4
Scott A Scholz, Ian Graves, Jeremy J Minty, Xiaoxia Nina Lin
Consolidated bioprocessing is a potential breakthrough technology for reducing costs of biochemical production from lignocellulosic biomass. Production of cellulase enzymes, saccharification of lignocellulose and conversion of the resulting sugars into a chemical of interest occur simultaneously within a single bioreactor. In this study, synthetic fungal consortia composed of the cellulolytic fungus Trichoderma reesei and the production specialist Rhizopus delemar demonstrated conversion of microcrystalline cellulose (MCC) and alkaline pre-treated corn stover to fumaric acid in a fully consolidated manner without addition of cellulase enzymes or expensive supplements such as yeast extract...
December 2, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29190379/fungal-specific-cyp51-inhibitor-vt-1598-demonstrates-in-vitro-activity-against-candida-and-cryptococcus-species-endemic-fungi-including-coccidioides-species-aspergillus-species-and-rhizopus-arrhizus
#5
Nathan P Wiederhold, Hoja P Patterson, Bich Hue Tran, Christopher M Yates, Robert J Schotzinger, Edward P Garvey
Background: Invasive fungal infections, including those caused by yeasts, moulds and endemic organisms, can be significant causes of morbidity and mortality in immunocompromised hosts, those with multiple comorbidities and occasionally immunocompetent hosts. Current antifungal agents are often limited by drug toxicities, drug interactions or the development of resistance. VT-1598 is a novel tetrazole that has greater specificity for fungal Cyp51 than currently available triazoles and thus the potential for clinically significant drug interactions is reduced...
November 27, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29184190/bacterial-endosymbionts-influence-host-sexuality-and-reveal-reproductive-genes-of-early-divergent-fungi
#6
Stephen J Mondo, Olga A Lastovetsky, Maria L Gaspar, Nicole H Schwardt, Colin C Barber, Robert Riley, Hui Sun, Igor V Grigoriev, Teresa E Pawlowska
Many heritable mutualisms, in which beneficial symbionts are transmitted vertically between host generations, originate as antagonisms with parasite dispersal constrained by the host. Only after the parasite gains control over its transmission is the symbiosis expected to transition from antagonism to mutualism. Here, we explore this prediction in the mutualism between the fungus Rhizopus microsporus (Rm, Mucoromycotina) and a beta-proteobacterium Burkholderia, which controls host asexual reproduction. We show that reproductive addiction of Rm to endobacteria extends to mating, and is mediated by the symbiont gaining transcriptional control of the fungal ras2 gene, which encodes a GTPase central to fungal reproductive development...
November 29, 2017: Nature Communications
https://www.readbyqxmd.com/read/29178191/cutaneous-granuloma-caused-by-rhizopus-oryzae-with-a-novel-mutation-in-the-cybb-gene-in-a-monozygotic-male-twin
#7
Zhaoyang Wang, Jianxin He, Zigang Xu, Zhe Xu, Lin Ma
A 2-year-old Chinese boy presented to our institution with a large lump in the sacrococcygeal region for two months, along with fever for five days. The initial lesion presented as a red papule accompanied by itching, and then progressed to 10 cm in diameter (Fig. 1a). Five days previously, the patient had developed a fever. This article is protected by copyright. All rights reserved.
November 24, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/29162893/intrinsic-short-tailed-azole-resistance-in-mucormycetes-is-due-to-an-evolutionary-conserved-aminoacid-substitution-of-the-lanosterol-14%C3%AE-demethylase
#8
Rita Caramalho, Joel D A Tyndall, Brian C Monk, Thomas Larentis, Cornelia Lass-Flörl, Michaela Lackner
Mucormycoses are emerging and potentially lethal infections. An increase of breakthrough infections has been found in cohorts receiving short-tailed azoles prophylaxis (e.g. voriconazole (VCZ)). Although VCZ is ineffective in vitro and in vivo, long-tailed triazoles such as posaconazole remain active against mucormycetes. Our goal was to validate the molecular mechanism of resistance to short-tailed triazoles in Mucorales. The paralogous cytochrome P450 genes (CYP51 F1 and CYP51 F5) of Rhizopus arrhizus, Rhizopus microsporus, and Mucor circinelloides were amplified and sequenced...
November 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29159452/saprotrophic-soil-fungi-to-improve-phosphorus-solubilisation-and-release-in-vitro-abilities-of-several-species
#9
Andrea Ceci, Flavia Pinzari, Fabiana Russo, Oriana Maggi, Anna Maria Persiani
Modern agriculture is dependent on phosphate rock (PR), which is a nonrenewable resource. Improvement of phosphorus (P) availability for crops in agricultural soils represents a key strategy to slow down the depletion of PR. The aim of this study was to identify potential P biofertilisers among saprotrophic fungal species. We tested 30 fungal strains belonging to 28 taxa (4 Zygomycota and 24 Ascomycota) and with different life strategies. The study showed that many saprotrophic fungi have the ability to mobilise P from insoluble forms according to a variety of mechanisms...
November 20, 2017: Ambio
https://www.readbyqxmd.com/read/29154198/evaluation-of-antioxidant-dna-targeting-antimicrobial-and-cytotoxic-studies-of-imine-capped-copper-and-nickel-nanoparticles
#10
P Adwin Jose, J Dhaveethu Raja, M Sankarganesh, J Rajesh
In this work, we have synthesized pyrimidine derivatives of Schiff base ligand 2-(4,6-dimethoxypyrimidin-2-ylimino)methyl)-6-methoxyphenol (DPMM) stabilized copper nanoparticles (DPMM-CuNPs) and nickel nanoparticles (DPMM-NiNPs) by modified Brust-Schiffrin technique as two step phase transfer assisted method and confirmed by UV-Visible, SEM and TEM analysis. The free radical scavenging activity of DPMM, DPMM-CuNPs &DPMM-NiNPs with 2, 2'-diphenylpicryl hydrazyl (DPPH), hydrogen peroxide (H2O2) super oxide dismutase (SOD) and nitric oxide (NO) shows that the antioxidant activity of DPMM-CuNPs is higher than DPMM &DPMM-NiNPs...
November 6, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29144537/thermotolerant-mycobiota-of-israeli-soils
#11
Isabella Grishkan
Composition of thermotolerant mycobiota in the soil of Israeli deserts and northern territories was examined in spatiotemporal dynamics. A total of 165 species from 82 genera were isolated at 37 °C using the soil dilution plate method. Aspergilli (Aspergillus fumigatus and A. niger) and teleomorphic ascomycetes (Canariomyces notabilis, Chaetomium nigricolor, and Ch. strumarium) comprised the basic part of the thermotolerant communities. The desert communities remarkably differed from the northern communities by a much higher abundance of A...
November 16, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29136413/first-metagenomic-survey-of-the-microbial-diversity-in-bioaerosols-emitted-in-waste-sorting-plants
#12
Jodelle Degois, Frederic Clerc, Xavier Simon, Cyril Bontemps, Pierre Leblond, Philippe Duquenne
Waste sorting activities are source of occupational bioaerosol exposures that are associated with several health disorders. New analytical tools, based on next-generation sequencing (NGS) technologies, provide powerful methods to assess the microbial composition of bioaerosols. The objectives of the study were (i) to assess the feasibility and the repeatability of NGS-based biodiversity measurements and (ii) to study the microbial biodiversity using NGS in bioaerosols emitted in a waste sorting plant (WSP)...
November 10, 2017: Annals of Work Exposures and Health
https://www.readbyqxmd.com/read/29122529/rhinocerebral-mucormycosis-caused-by-rhizopus-arrhizus-var-tonkinensis
#13
Y Cheng, Y Gao, X Y Liu, G Y Wang, G Q Zhang, S Q Gao
Mucormycosis is a rare life-threatening opportunistic infection caused by fungi of the order Mucorales. We report a case of rhinocerebral mucormycosis caused by Rhizopus arrhizus var. tonkinensis, the first formally reported case in the literature. Early diagnosis, reversal of predisposing factors, surgical debridement and prompt administration of antifungal therapy are critical for good outcome.
November 6, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29117148/physical-structural-barrier-and-antifungal-characterization-of-chitosan-zein-edible-films-with-added-essential-oils
#14
Monserrat Escamilla-García, Georgina Calderón-Domínguez, Jorge J Chanona-Pérez, Angélica G Mendoza-Madrigal, Prospero Di Pierro, Blanca E García-Almendárez, Aldo Amaro-Reyes, Carlos Regalado-González
Edible films (EFs) have gained great interest due to their ability to keep foods safe, maintaining their physical and organoleptic properties for a longer time. The aim of this work was to develop EFs based on a chitosan-zein mixture with three different essential oils (EOs) added: anise, orange, and cinnamon, and to characterize them to establish the relationship between their structural and physical properties. The addition of an EO into an EF significantly affected (p < 0.05) the a* (redness/greenness) and b* (yellowness/blueness) values of the film surface...
November 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29100947/evaluation-of-pcr-reverse-line-blot-hybridization-assay-for-simultaneous-identification-of-medically-important-saprophytic-fungi
#15
S Agha Kuchak Afshari, H Rahimi, S J Hashemi, R Daie Ghazvini, H Badali, S Aghaei Gharehbolagh, S Rezaie
BACKGROUND: In immunocompromised patients suffering from invasive fungal infections, rapid identification of fungal species is important since the appropriate treatment is usually related to the responsible species. We describe here, an assay based on combination of PCR and reverse line blot hybridization (PCR/RLB) for differentiation causative agent of fungal infections. MATERIALS AND METHODS: We performed PCR/RLB assay on 10 reference strains, which include Aspergillus species (A...
October 31, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29089855/solid-state-fermentation-reduces-phytic-acid-level-improves-the-profile-of-myo-inositol-phosphates-and-enhances-the-availability-of-selected-minerals-in-flaxseed-oil-cake
#16
Robert Duliński, Bożena Stodolak, Łukasz Byczyński, Aleksander Poreda, Anna Starzyńska-Janiszewska, Krzysztof Żyła
Flaxseed oil cake was subjected to fermentation with Rhizopus oligosporus (DSM 1964 and ATCC 64063), and the phytate (InsP6) content, myo-inositol phosphate profile and in vitro bioavailability of essential minerals were studied. Flaxseed oil cake had a phytate mass fraction of 13.9 mg/g. A 96-hour fermentation of flaxseed oil cake by R. oligosporus DSM 1964 and R. oligosporus ATCC 64063 decreased the InsP6 content by 48 and 33%, respectively. The strains had different phytate-degrading activities: fermentation of flaxseed oil cake with R...
September 2017: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/29083057/convenient-enzymatic-resolution-of-r-s-2-methylbutyric-acid-catalyzed-by-immobilized-lipases
#17
Mateus Mittersteiner, Bruna Luiza Linshalm, Ana Paula Furlan Vieira, Patrícia Bulegon Brondani, Dilamara Riva Scharf, Paulo Cesar de Jesus
The application of several immobilized lipases has been explored in the enantioselective esterification of (R,S)-2-methylbutyric acid, an insect pheromone precursor. With the use of Candida antarctica B, using hexane as solvent, (R)-pentyl 2-methylbutyrate was prepared in 2 h with c 40%, eep 90%, and E = 35, while Thermomyces lanuginosus leads to c 18%, eep 91%, and E = 26. The (S)-enantiomer was obtained by the use of Candida rugosa or Rhizopus oryzae (2-h reaction, c 34% and 35%, eep 75 and 49%, and E = 10 and 4, respectively)...
October 30, 2017: Chirality
https://www.readbyqxmd.com/read/29081085/identification-by-pcr-dgge-sds-page-and-maldi-tof-ms-of-lab-and-fungi-in-laru-a-fermented-starter
#18
Lenny S F Ahmadsah, Eiseul Kim, Youn-Sik Jung, Hae-Yeong Kim
Laru samples obtained from the upper part of Borneo Island were analyzed for their lactic acid bacterial (LAB) and fungal diversity using both a culture-independent method (PCR-DGGE) and culture-dependent methods (SDS-PAGE and MALDI-TOF MS). Pediococcus pentosaceus, Lactobacillus brevis, Saccharomycopsis fibuligera, Hyphopichia burtonii, and Kodamaea ohmeri were detected by all three methods. In addition, Weissella cibaria, Weissella paramesenteroides, Leuconostoc citreum, Leuconostoc mesenteroides, Lactococcus lactis, Rhizopus oryzae/Amylomyces rouxii, Mucor indicus, and Candida intermedia were detected by PCR-DGGE...
October 30, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29080618/antimicrobial-activity-of-water-and-acetone-extracts-of-some-eucomis-taxa
#19
Małgorzata Mizielińska, Piotr Salachna, Magdalena Ordon, Łukasz Łopusiewicz
OBJECTIVE: To evaluate the antibacterial and antifungal properties of acetone and water extracts of selected Eucomis taxa. METHODS: The bulbs of Eucomis bicolor, Eucomis comosa (E. comosa) and E. comosa cv. were dried and examples from each experimental treatment were ground separately into powder. Each sample was divided into two groups with equal amounts of powder, and respectively extracted with water and a 70% solution of aqueous acetone. The crude water extracts were then filtered through a 0...
September 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29075326/sustainable-carbon-sources-for-microbial-organic-acid-production-with-filamentous-fungi
#20
Stefan Dörsam, Jana Fesseler, Olga Gorte, Thomas Hahn, Susanne Zibek, Christoph Syldatk, Katrin Ochsenreither
BACKGROUND: The organic acid producer Aspergillus oryzae and Rhizopus delemar are able to convert several alternative carbon sources to malic and fumaric acid. Thus, carbohydrate hydrolysates from lignocellulose separation are likely suitable as substrate for organic acid production with these fungi. RESULTS: Before lignocellulose hydrolysate fractions were tested as substrates, experiments with several mono- and disaccharides, possibly present in pretreated biomass, were conducted for their suitability for malic acid production with A...
2017: Biotechnology for Biofuels
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