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https://www.readbyqxmd.com/read/28317745/the-impact-of-rhizopus-oryzae-cultivation-on-rice-bran-gamma-oryzanol-recovery-and-its-antioxidant-properties
#1
Kelly Cristina Massarolo, Taiana Denardi de Souza, Carolina Carvalho Collazzo, Eliana Badiale Furlong, Leonor Almeida de Souza Soares
This study evaluated the effect of the solid state cultivation (SSC) time of rice bran by Rhizopus oryzae on γ-oryzanol recovery and its antioxidant properties. Gamma-oryzanol was extracted with organic solvents and its extracts were characterized by GC-FID and HPLC-UV. The antioxidant capacity was assessed by DPPH and ABTS(+) assays, β-carotene/linoleic acid system, and reduction of oxidation in lipid system. The biomass showed the γ-oryzanol recovery increased by 51.5% (20.52mg/g), and 5.7% in polyunsaturated fatty acids...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28295011/increased-dosage-of-aox1-promoter-regulated-expression-cassettes-leads-to-transcription-attenuation-of-the-methanol-metabolism-in-pichia-pastoris
#2
Elena Cámara, Nils Landes, Joan Albiol, Brigitte Gasser, Diethard Mattanovich, Pau Ferrer
The methanol-regulated alcohol oxidase promoter (PAOX1) of Pichia pastoris is one of the strongest promoters for heterologous gene expression in this methylotrophic yeast. Although increasing gene dosage is one of the most common strategies to increase recombinant protein productivities, the increase of gene dosage of Rhizopus oryzae lipase (ROL) in P. pastoris has been previously shown to reduce cell growth, lipase production and substrate consumption in high-copy strains. To better assess that physiological response, transcriptomics analysis was performed of a subset of strains with 1 to 15 ROL copies...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28290330/determination-of-the-fungal-community-of-pit-mud-in-fermentation-cellars-for-chinese-strong-flavor-liquor-using-dgge-and-illumina-miseq-sequencing
#3
Maoke Liu, Yuming Tang, Ke Zhao, Ying Liu, Xiaojiao Guo, Daoqun Ren, Wanchun Yao, Xinhui Tian, Yunfu Gu, Bin Yi, Xiaoping Zhang
Chinese strong-flavor liquor (CSFL) is fermented in cellars lined with pit mud (PM). This PM, specific fermented clay, contains microbes that play important roles in CSFL production. However, there is limited information about fungal community structure and cellar-age-related changes in PM. In this study, PM samples were removed from the cellars used for 5 and 100years and characterized using denaturing gradient gel electrophoresis (DGGE) and Illumina MiSeq sequencing. Both methods revealed there were no significant differences in fungal species diversity (Shannon index, Chao1, and observed species) between the 5- and 100-year PM samples (p>0...
January 2017: Food Research International
https://www.readbyqxmd.com/read/28264840/prophylaxis-with-isavuconazole-or-posaconazole-protect-immunosuppressed-mice-from-pulmonary-mucormycosis
#4
Teclegiorgis Gebremariam, Sondus Alkhazraji, Clara Baldin, Laura Kovanda, Nathan P Wiederhold, Ashraf S Ibrahim
We assessed prophylactic or continuous therapy of isavuconazole, posaconazole or voriconazole in treating pulmonary murine mucormycosis. In the prophylaxis studies, only isavuconazole treatment resulted in significantly improved survival and lowered tissue fungal burden of immunosuppressed mice infected with Rhizopus delemar. In the continuous treatment studies, isavuconazole and posaconazole, but not voriconazole, equally prolonged survival time and lowered tissue fungal burden versus placebo-treated mice...
March 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28258993/co-fermentation-of-a-mixture-of-glucose-and-xylose-to-fumaric-acid-by-rhizopus-arrhizus-rh-7-13-9
#5
Huan Liu, Huirong Hu, Yuhan Jin, Xuemin Yue, Li Deng, Fang Wang, Tianwei Tan
Lignocellulose is the most abundant biomass, composed of cellulose, hemicellulose and lignin. It can be converted into glucose and xylose, which could be utilized as carbon source to produce fumaric acid. But glucose and xylose were commonly used separately to produce fumaric acid, while the co-fermentation of glucose and xylose process was not studied so far. In this work, the co-fermentation process was researched through a new strain R. arrhizus RH 7-13-9# isolated from high concentration xylose. It was firstly proven to utilize glucose efficiently and 37...
February 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28256389/analysis-of-microbial-diversity-in-shenqu-with-different-fermentation-times-by-pcr-dgge
#6
Tengfei Liu, Tianzhu Jia, Jiangning Chen, Xiaoyu Liu, Minjie Zhao, Pengpeng Liu
Shenqu is a fermented product that is widely used in traditional Chinese medicine (TCM) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. The aim of this study was to investigate microbial diversity in Shenqu using different fermentation time periods. DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles indicated that a strain of Pediococcus acidilactici (band 9) is the predominant bacteria during fermentation and that the predominant fungi were uncultured Rhizopus, Aspergillus oryzae, and Rhizopus oryzae...
January 22, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28231081/improving-bread-quality-with-the-application-of-a-newly-purified-thermostable-%C3%AE-amylase-from-rhizopus-oryzae-fsis4
#7
Amel Ait Kaki El-Hadef El-Okki, Mohammed Gagaoua, Hayat Bourekoua, Kahina Hafid, Leila Bennamoun, Shahrazed Djekrif-Dakhmouche, Mohamed El-Hadef El-Okki, Zahia Meraihi
A new thermostable α-amylase from Rhizopus oryzae FSIS4 was purified for first time and recovered in a single step using a three-phase partitioning (TPP) system. The fungal α-amylase, at a concentration of 1.936 U per kg of flour, was used in bread-making and compared to the commercial enzyme. The results showed a significant effect of the recovered α-amylase in the prepared bread and allowed us to improve the quality of the bread. The study indicated clearly that the recovered α-amylase is a potential candidate for future applications in the bread-making industry and in other food biotechnology applications...
January 1, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28229207/engineering-rtca-pathway-and-c4-dicarboxylate-transporter-for-l-malic-acid-production
#8
Xiulai Chen, Yuancai Wang, Xiaoxiang Dong, Guipeng Hu, Liming Liu
L-Malic acid is an important component of a vast array of food additives, antioxidants, disincrustants, pharmaceuticals, and cosmetics. Here, we presented a pathway optimization strategy and a transporter modification approach to reconstruct the L-malic acid biosynthesis pathway and transport system, respectively. First, pyruvate carboxylase (pyc) and malate dehydrogenase (mdh) from Aspergillus flavus and Rhizopus oryzae were combinatorially overexpressed to construct the reductive tricarboxylic acid (rTCA) pathway for L-malic acid biosynthesis...
February 22, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28221876/effect-of-magnesium-acetate-on-the-antimold-activity-of-lactobacillus
#9
Katarzyna Kycia, Anna Bzducha-Wróbel, Karolina Kraśniewska, Anna Chlebowska-Śmigiel, Małgorzata Gniewosz
The antimold activity of lactic acid bacteria (LAB) is used in food biopreservation. The aim of this study was to evaluate the effect of magnesium acetate added to de Man Rogosa Sharpe (MRS) medium on the antimold activity of three LAB strains ( Lactobacillus plantarum , Lactobacillus brevis , and Lactobacillus fermentum ) against molds contaminating food ( Aspergillus oryzae , Aspergillus niger , Penicillium chrysogenum , Fusarium avenaceum , and Rhizopus arrhizus ) and their ability to produce organic acids (acetic acid, lactic acid, and phenyllactic acid)...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28205239/bioprocessing-of-common-beans-in-diets-for-tilapia-in-vivo-digestibility-and-antinutritional-factors
#10
Francisco Valdez-González, Roberto Gutiérrez-Dorado, Alfredo Hernández-Llamas, Manuel García-Ulloa, Luís Sánchez-Magaña, Breidy Cuevas-Rodríguez, Hervey Rodríguez-González
BACKGROUND: Bioprocessing of ingredients by solid-state fermentation is a low-cost technique for preparing diets. It is performed by adding microorganisms such as Rhizopus oligosporus to bean grains, achieving minimal degradation of nutrients and a significant improvement in digestibility. In particular, fermentation induces favorable changes in beans by reducing enzyme inhibitors, such as phytates and tannins. RESULTS: Fermentation significantly (P < 0.05) increased the protein content and digestibility of dry matter and protein compared with whole bean grains, and decreased the content of lipids, ash and phytic acid...
February 16, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28199672/identification-of-a-novel-rhizopus-specific-antigen-by-screening-with-a-signal-sequence-trap-and-evaluation-as-a-possible-diagnostic-marker-of-mucormycosis
#11
Kanako Sato, Ken-Ichi Oinuma, Mamiko Niki, Satoshi Yamagoe, Yoshitsugu Miyazaki, Kazuhisa Asai, Koichi Yamada, Kazuto Hirata, Yukihiro Kaneko, Hiroshi Kakeya
No abstract text is available yet for this article.
February 11, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28159792/molecular-characterization-and-phylogenetic-analysis-of-two-novel-fungal-phenolic-udp-glycosyltransferases-from-absidia-coerulea-and-rhizopus-japonicus
#12
Kebo Xie, Xiaoxiang Dou, Ridao Chen, Dawei Chen, Cheng Fang, Zhiyan Xiao, Jungui Dai
In the present study, two novel phenolic UDP-glycosyltransferases (P-UGTs), UGT58A1 and UGT59A1, which can transfer sugar moieties from active donors to phenolic acceptors to generate corresponding glycosides, were identified from the fungi kingdom. UGT58A1 (from Absidia coerulea) and UGT59A1 (from Rhizopus japonicas) share a low degree of homology with known UGTs from animals, plants, bacteria, and virus. These two P-UGTs are membrane-bound proteins with an N-terminal signal peptide and a transmembrane domain at the C-terminus...
February 3, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28159232/bidirectional-immunomodulating-activity-of-fermented-polysaccharides-from-yupingfeng
#13
Hao Sun, Xueqin Ni, Dong Zeng, Fuqin Zou, Mingyue Yang, Zhirong Peng, Yi Zhou, Yan Zeng, Hui Zhu, Hesong Wang, Zhongqiong Yin, Kangcheng Pan, Bo Jing
Accumulating evidence has proven the immunomodulating activity of Yupingfeng. This study compared the immunomodulatory activity in vitro of the unfermented Yupingfeng dreg polysaccharides (UYDP) with that of the fermented Yupingfeng dreg polysaccharides (FYDP) obtained using Rhizopus oligosporus SH. Results consistently elucidated the duality of the immunomodulatory roles of UYDP and FYDP in regulating proliferation, and cytokines expressions in murine lymphocytes and macrophages. Compared with UYDP, FYDP effectively enhanced the proliferation of lymphocytes and promoted mRNA expression of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, INF-γ, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and TLR-4 in lymphocytes and macrophages...
February 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28143744/can-fungi-compete-with-marine-sources-for-chitosan-production
#14
REVIEW
V Ghormade, E K Pathan, M V Deshpande
Chitosan, a β-1,4-linked glucosamine polymer is formed by deacetylation of chitin. It has a wide range of applications from agriculture to human health care products. Chitosan is commercially produced from shellfish, shrimp waste, crab and lobster processing using strong alkalis at high temperatures for long time periods. The production of chitin and chitosan from fungal sources has gained increased attention in recent years due to potential advantages in terms of homogenous polymer length, high degree of deacetylation and solubility over the current marine source...
January 28, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28140581/high-throughput-analysis-of-enzymatic-hydrolysis-of-biodegradable-polyesters-by-monitoring-co-hydrolysis-of-a-polyester-embedded-fluorogenic-probe
#15
Michael Thomas Zumstein, Hans-Peter E Kohler, Kristopher McNeill, Michael Sander
Biodegradable polyesters have the potential to replace non-degradable, persistent polymers in numerous applications and thereby alleviate plastic accumulation in the environment. Herein we present an analytical approach to study enzymatic hydrolysis of polyesters, the key step in their overall biodegradation process. The approach is based on embedding fluorescein dilaurate (FDL), a fluorogenic ester substrate, into the polyester matrix and on monitoring the enzymatic co-hydrolysis of FDL to fluorescein during enzymatic hydrolysis of the polyester...
January 31, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28133596/hydrolysis-of-carotenoid-esters-from-tagetes-erecta-by-the-action-of-lipases-from-yarrowia-lipolytica
#16
Abraham Figueiras Abdala, Alfonso Pérez Gallardo, Lorenzo Guevara Olvera, Eleazar Máximo Escamilla Silva
The present study was conducted to evaluate the feasibility of enzymatic hydrolysis of carotenoid esters from Tagetes erecta using lipases from the yeast of Yarrowia lipolytica, with the aim of obtaining free lutein. The optimal concentrations of seven nutrients, considering the production of lipases relative to biomass (Yp/x) as the response variable, were determined in flask fermentations. In addition, we studied the effect on hydrolysis of growing Y. lipolytica in the presence of the oleoresin of the marigold flower in flask and stirred tank...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28100314/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-rapid-identification-of-fungal-rhinosinusitis-pathogens
#17
Xinxin Lu, Yanfei Huang, Mingxin Zhang, Min Zhu, Mei Wang, Yufeng Sun, Haitong Gu, Jingjing Cao, Xue Li, Shaoya Zhang, Jinglin Wang
BACKGROUND: filamentous fungi are the most important pathogens causing fungal rhinosinusitis (FRS). Current laboratory diagnosis of FRS pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time-consuming and expertise-dependent. Although matrix -assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been employed to identify various fungi, its performance for the identification of FRS fungi is less clear...
January 17, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28053593/evaluation-of-aerial-microbial-pollutants-in-al-haram-al-nabawi-during-pilgrimage-of-2013
#18
Kholoud M Alananbeh, Nahla Boquellah, Nadia Al Kaff, Majid Al Ahmadi
Al-Madinah Al-Munawwarah is the second holiest site in Islam. The possibility of new emerging microbes is valid due to the increased number of pilgrims. The objectives of the current study were to estimate the numbers of fungi and bacteria inside and outside Al-Haram Al-Nabawi and to find whether new bacterial and fungal species have emerged compared to previous studies. Air samples were collected twice a day from 12 spots and four directions during the pilgrim year of 2013 for four consecutive weeks by using the sedimentation method...
January 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28053592/extracellular-biosynthesis-of-silver-nanoparticles-using-rhizopus-stolonifer
#19
Khalid AbdelRahim, Sabry Younis Mahmoud, Ahmed Mohamed Ali, Khalid Salmeen Almaary, Abd El-Zaher M A Mustafa, Sherif Moussa Husseiny
Synthesis of silver nanoparticles (AgNPs) has become a necessary field of applied science. Biological method for synthesis of AgNPs by Rhizopus stolonifer aqueous mycelial extract was used. The AgNPs were identified by UV-visible spectrometry, X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectrometry (FT-IR). The presence of surface plasmon band around 420 nm indicates AgNPs formation. The characteristic of the AgNPs within the face-centered cubic (fcc) structure are indicated by the peaks of the X-ray diffraction (XRD) pattern corresponding to (1 1 1), (2 0 0) and (2 2 0) planes...
January 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28017541/production-of-flavor-compounds-from-olive-mill-waste-by-rhizopus-oryzae-and-candida-tropicalis
#20
Onur Guneser, Asli Demirkol, Yonca Karagul Yuceer, Sine Ozmen Togay, Muge Isleten Hosoglu, Murat Elibol
The purpose of this study was to investigate the production of flavor compounds from olive mill waste by microbial fermentation of Rhizopus oryzae and Candida tropicalis. Olive mill waste fermentations were performed in shake and bioreactor cultures. Production of flavor compounds from olive mill waste was followed by Gas Chromatography-Mass spectrometry, Gas chromatography- olfactometry and Spectrum Sensory Analysis(®). As a result, 1.73-log and 3.23-log cfu/mL increases were observed in the microbial populations of R...
November 23, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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