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https://www.readbyqxmd.com/read/28714266/potential-biotechnological-capabilities-of-cultivable-mycobiota-from-carwash-effluents
#1
Timothy Sibanda, Ramganesh Selvarajan, Memory Tekere, Hlengilizwe Nyoni, Stephen Meddows-Taylor
Urban life has created man-made extreme environments like carwashes. These environments have, however, not been sufficiently explored for mycobiota that can be sources of biotechnologically useful products, as has been the case with natural extreme environments. Using a combination of culture and molecular techniques, fungi from carwash effluents was characterized for production of lipase and cellulase enzymes, nonpolar and polar biotechnologically relevant secondary metabolites and hydrocarbon utilization...
July 17, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28711611/biochemical-characterization-and-stability-assessment-of-rhizopus-oryzae-lipase-covalently-immobilized-on-amino-functionalized-magnetic-nanoparticles
#2
Kh Pashangeh, M Akhond, H R Karbalaei-Heidari, G Absalan
Amino-functionalized magnetic nanoparticles (Fe3O4) have been investigated as a support for covalent immobilization of lipase. The nanoparticles were prepared by chemical coprecipitation method and subsequently were coated with 3-aminopropyltriethoxysilane (APTES) via silanization reaction. With glutaraldehyde, as the coupling agent, the lipase from Rhizopus oryzae was successfully immobilized onto the amino-functionalized magnetic nanoparticles. The synthesized support was characterized by transmission electron microscopy and Fourier transform infrared spectroscopy...
July 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28711520/exploring-substrate-specificities-of-a-recombinant-rhizopus-oryzae-lipase-in-biodiesel-synthesis
#3
Albert Canet, M Dolors Benaiges, Francisco Valero, Patrick Adlercreutz
The alcoholysis of triolein was used to explore the specific features of a recombinant Rhizopus oryzae lipase (rROL) for biodiesel synthesis. For this purpose, different acylglycerols were compared as substrates in lipase-catalysed transesterification. rROL was shown to exhibit a higher specificity towards 1-monoolein than triolein compared to other R. oryzae lipases, being more than 4-fold more specific; in contrast, rROL did not accept 2-monoolein as substrate, concluding that it is highly 1,3-positional specific...
July 12, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28702025/cellular-and-molecular-defects-underlying-invasive-fungal-infections-revelations-from-endemic-mycoses
#4
REVIEW
Pamela P Lee, Yu-Lung Lau
The global burden of fungal diseases has been increasing, as a result of the expanding number of susceptible individuals including people living with human immunodeficiency virus (HIV), hematopoietic stem cell or organ transplant recipients, patients with malignancies or immunological conditions receiving immunosuppressive treatment, premature neonates, and the elderly. Opportunistic fungal pathogens such as Aspergillus, Candida, Cryptococcus, Rhizopus, and Pneumocystis jiroveci are distributed worldwide and constitute the majority of invasive fungal infections (IFIs)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28685375/higher-in-vitro-proliferation-rate-of-rhizopus-oryzae-in-blood-of-diabetic-individuals-in-chronic-glycaemic-control-compared-with-non-diabetic-individuals
#5
Grace Salazar-Tamayo, Luis E López-Jácome, Jesús Resendiz-Sanchez, Rafael Franco-Cendejas, Patricia Rodriguez-Zulueta, Dora E Corzo-León
Metabolic control improves outcomes associated with mucormycosis. The aim of this study was to compare the in vitro proliferation of Rhizopus oryzae in blood of individuals with and without diabetes at different glycaemic levels. Ninety-five individuals were included. Blood samples from each participant were incubated with sporangiospores of R. oryzae. The germination, filamentation and growth of R. oryzae were compared at different time points. Four groups were defined, one without (group A, n = 30) and three with diabetes: group B (HbA1c ≤7%, N = 24), group C (HbA1c 7...
July 6, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28681607/n-glycosylation-engineering-to-improve-the-constitutive-expression-of-rhizopus-oryzae-lipase-in-komagataella-phaffii
#6
Xiaowei Yu, Min Yang, Chuanhuan Jiang, Xiaofeng Zhang, Yan Xu
Our previous studies demonstrated that the N-glycans in Rhizopus chinensis lipase (RCL) was important for its secretion. In order to improve the secretion of Rhizopus oryzae lipase (ROL) under the control of the GAP promoter in K. phaffii, two extra N-glycosylation sites were introduced in ROL according to the position of the N-glycosylation sites of RCL by sequence alignment. The results indicated that the secretion level of ROL was strongly improved by N-glycosylation engineering, and the highest value of extracellular enzyme activity was increased from 0...
July 6, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28681022/aeromycological-analysis-of-allergenic-airborne-fungi-in-qazvin-iran
#7
S A Ghiasian, A H Maghsood, M R Aghamirian
BACKGROUND AND PURPOSE: Airborne fungi are one of the most important agents responsible for triggering allergicreactions such as rhinitis and severe asthma. This study was conducted to analyze and monitor the prevalence and distribution patterns of atmospheric fungal aerosols in the air of Qazvin during winter of 2012. MATERIALS AND METHODS: In the current descriptive study, the incidence and diversity of potentially allergenic airborne fungi were determined using two times sampling interval in 25 different locations of Qazvin city by Petri dish trappingtechnique and exposure of 10- cm diameter plates of Sabouraud's dextrose agar medium plus chloramphenicol to the air...
September 2016: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/28673517/semi-continuous-production-of-high-activity-pectinases-by-immobilized-rhizopus-oryzae-using-tobacco-wastewater-as-substrate-and-their-utilization-in-the-hydrolysis-of-pectin-containing-lignocellulosic-biomass-at-high-solid-content
#8
REVIEW
Yu-Xi Zheng, Yuan-Liang Wang, Jun Pan, Jian-Rong Zhang, Ya Dai, Kun-Yan Chen
In this study, highly reactive endo- and exo-polygalacturonases (PGs) were produced from the tobacco industry wastewater using immobilized Rhizopus oryzae. Compared with free cells, immobilized cells increased enzyme activity 2.8-fold and reduced production time to 24h by shake-flask production. Moreover, the immobilized cells enabled the semi-continuous production of enzymes through repeated-batch mode for seven consecutive cycles in a scale-up bioreactor. During the first five cycles, the average endo-PG and exo-PG activities reached 307...
October 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28662421/preparation-of-hydrolytic-liquid-from-dried-distiller-s-grains-with-solubles-and-fumaric-acid-fermentation-by-rhizopus-arrhizus-rh-7-13
#9
Huan Liu, Xuemin Yue, Yuhan Jin, Meng Wang, Li Deng, Fang Wang, Tianwei Tan
Fumaric acid production from lignocellulosic materials is an alternative chemicals production system. This work investigated the suitable conditions for hydrolysis of dried distiller's grains with solubles (DDGS). The hydrolytic liquid was subsequently used for the production of fumaric acid. After optimizing the hydrolysis conditions, the most suitable concentration of H2SO4 (2%), hydrolysis temperature (120 °C), hydrolysis time (100min) and solid/liquid ratio (1:10) were obtained. The yield of monosaccharides reached 258 mg/g DDGS and 15...
June 26, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28652241/prophylactic-treatment-with-vt-1161-protects-immunosuppressed-mice-from-rhizopus-arrhizus-var-arrhizus-infection
#10
Teclegiorgis Gebremariam, Sondus Alkhazraji, Lin Lin, Nathan P Wiederhold, Edward P Garvey, William J Hoekstra, Robert J Schotzinger, Thomas F Patterson, Scott G Filler, Ashraf S Ibrahim
We compared prophylactic or continuous therapy of the investigational drug VT-1161 to posaconazole in treating murine mucormycosis due to Rhizopus arrhizus var. arrhizus In the prophylaxis studies, only VT-1161 resulted in improved survival and lowered tissue fungal burden of immunosuppressed infected mice. In the continuous therapy, VT-1161 outperformed posaconazole in prolonging mice survival time despite their comparable effect in lowering tissue fungal burden. These results support the further development of VT-1161 against mucormycosis...
June 26, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28651077/rhizopus-oryzae-ancient-microbial-resource-with-importance-in-modern-food-industry
#11
REVIEW
Liliana Londoño-Hernández, Cristina Ramírez-Toro, Héctor A Ruiz, Juan A Ascacio-Valdés, Miguel A Aguilar-Gonzalez, Raúl Rodríguez-Herrera, Cristóbal N Aguilar
Filamentous fungi are microorganisms widely known for their diverse biochemical features. Fungi can efficiently invade a wide variety of substrates under operational conditions producing numerous bioproducts of interest, such as enzymes, organic acids, aromatic compounds and colorants. An additional interesting characteristic of some fungi is their safety classification for different uses, which guarantees that the bioproducts obtained from them do not contain any toxic component deleterious to humans. Rhizopus oryzae is among this group of fungi and is classified as a GRAS filamentous fungus, commonly used for production of some oriental traditional foods...
June 15, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28646276/rhizopus-arrhizus-invasive-infection-due-to-self-inflicted-scratch-injuries-in-a-diabetic-patient-with-non-ketotic-acidosis
#12
Luis Buzón Martín, Miguel Ángel Morán Rodríguez, Nicole Mercier, Maria Ortega Lafont, Eva Ojeda Fernández, Ana Ruiz de la Parte, Miguel Estefanía
Mucormycosis is a rare infection caused by members included in the subphylum Mucoromycotina. Characterized by the histopathological hallmark of angioinvasion, these infections affect most often patients with certain underlying conditions carrying immunosuppression (haematological neoplasias, diabetic ketoacidosis and other forms of acidosis, and iron overload) or immunocompetent patients with traumatic mucocutaneous barriers breakdown and direct inoculation of the mould. A case is presented in which a rare underlying condition (non-ketotic acidosis) and a rare cause of cutaneous injuries collide...
June 23, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28636431/enhanced-production-of-raw-starch-degrading-enzyme-using-agro-industrial-waste-mixtures-by-thermotolerant-rhizopus-microsporus-for-raw-cassava-chip-saccharification-in-ethanol-production
#13
Srisakul Trakarnpaiboon, Nantana Srisuk, Kuakoon Piyachomkwan, Kenji Sakai, Vichien Kitpreechavanich
In the present study, solid state fermentation for the production of raw starch degrading enzyme was investigated by thermotolerant Rhizopus microsporus TISTR 3531 using a combination of agro-industrial wastes as substrates. The obtained crude enzyme was applied for hydrolysis of raw cassava starch and chips at low temperature and subjected to non-sterile ethanol production using raw cassava chips. The agro-industrial wastes ratio was optimized using a simplex axial mixture design. The results showed that the substrate mixture consisting of rice bran: corncob: cassava bagasse at 8 g: 10 g: 2 g yielded the highest enzyme production of 201...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28623532/rhizopus-homothallicus-causing-invasive-infections-series-of-three-cases-from-a-single-centre-in-north-india
#14
Prathyusha Kokkayil, Mragnayani Pandey, Reshu Agarwal, Pratibha Kale, Gagandeep Singh, Immaculata Xess
Mucormycoses are opportunistic fungal infections with a high mortality rate. Rhizopus oryzae is the most common agent implicated in human infections. Although R. homothallicus has been previously reported to be a cause of pulmonary mucormycosis, it is the first time that we are reporting as a causative agent of rhino-orbital and cutaneous mucormycosis.
June 16, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28621110/characterization-of-microbial-communities-in-chinese-rice-wine-collected-at-yichang-city-and-suzhou-city-in-china
#15
Yucai Lyu, Yanli Gong, Yajie Li, Zejiang Pan, Yi Yao, Ning Li, Jinling Guo, Dachun Gong, Yihong Ti, Caiyun Peng
Two typical microbial communities from Chinese rice wine fermentation collected in Yichang city and Suzhou city in China were investigated. Both communities could ferment glutinous rice to rice wine in 2 d. The sugar and ethanol contents were 198.67 and 14.47 mg/g, respectively, for rice wine from Yichang city, and 292.50 and 12.31 mg/g, respectively, for rice wine from Suzhou city. Acetic acid and lactic acid were the most abundant organic acids. Abundant fungi and bacteria were detected in both communities by high-throughput sequencing...
June 16, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28616054/an-investigation-on-non-invasive-fungal-sinusitis-molecular-identification-of-etiologic-agents
#16
Abdolrasoul Mohammadi, Seyed Mostafa Hashemi, Sayed Hamidreza Abtahi, Seyed Mohammad Lajevardi, Sahar Kianipour, Rasoul Mohammadi
BACKGROUND: Fungal sinusitis is increasing worldwide in the past two decades. It is divided into two types including invasive and noninvasive. Noninvasive types contain allergic fungal sinusitis (AFS) and fungus ball. AFS is a hypersensitivity reaction to fungal allergens in the mucosa of the sinonasal tract in atopic individuals. The fungus ball is a different type of noninvasive fungal rhinosinusitis which is delineated as an accumulation of debris and fungal elements inside a paranasal sinus...
2017: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/28610916/the-rhizoferrin-biosynthetic-gene-in-the-fungal-pathogen-rhizopus-delemar-is-a-novel-member-of-the-nis-gene-family
#17
Cassandra S Carroll, Clark L Grieve, Indu Murugathasan, Andrew J Bennet, Clarissa M Czekster, Huanting Liu, James Naismith, Margo M Moore
Iron is essential for growth and in low iron environments such as serum many bacteria and fungi secrete ferric iron-chelating molecules called siderophores. All fungi produce hydroxamate siderophores with the exception of Mucorales fungi, which secrete rhizoferrin, a polycarboxylate siderophore. Here we investigated the biosynthesis of rhizoferrin by the opportunistic human pathogen, Rhizopus delemar. We searched the genome of R. delemar 99-880 for a homologue of the bacterial NRPS-independent siderophore (NIS) protein, SfnaD, that is involved in biosynthesis of staphyloferrin A in Staphylococcus aureus...
June 10, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28596926/invasive-mucormycosis-induced-pneumopericardium-a-rare-cause-of-pneumopericardium-in-an-immunocompromised-patient
#18
Sana Khan, Muhammad Waqar Elahi, Waqas Ullah, Hafez Mohammad Ammar Abdullah, Ejaz Ahmad, Mayar Al Mohajer, Aneela Majeed
Mucor and Rhizopus cause life-threatening infections primarily involving the lungs and sinuses, which disseminate very rapidly by necrosis and infarction of the contiguous tissues. We present a case of a 64-year-old African American posttransplant patient who presented with a productive cough and weight loss. He had a past surgical history of renal transplant for renal cell carcinoma and was on dual immunosuppressive therapy, that is, mycophenolate and tacrolimus. During his hospital stay, he developed a pneumopericardium due to the direct extension of a lung lesion...
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/28595608/antimicrobial-activity-toxicity-and-anti-inflammatory-potential-of-methanolic-extracts-of-four-ethnomedicinal-plant-species-from-punjab-pakistan
#19
Rabia Naz, Hafsa Ayub, Sajid Nawaz, Zia Ul Islam, Tayyaba Yasmin, Asghari Bano, Abdul Wakeel, Saqib Zia, Thomas H Roberts
BACKGROUND: The plant species Aristolochia indica (AI), Melilotus indicus (MI), Tribulus terrestris (TT) and Cuscuta pedicellata (CP) are widely used in folk medicine in the villages around Chowk Azam, South Punjab, Pakistan. The aim of this study was to evaluate the antioxidant activity, phytochemical composition, and the antibacterial, antifungal, cytotoxic and anti-inflammatory potential of the four medicinal plants listed above. For CP stem, this study represents (to the best of our knowledge) the first time phytochemicals have been identified and the antioxidant and anti-inflammatory potential determined...
June 8, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28595086/evaluation-of-different-pcr-primers-for-denaturing-gradient-gel-electrophoresis-dgge-analysis-of-fungal-community-structure-in-traditional-fermentation-starters-used-for-hong-qu-glutinous-rice-wine
#20
Xu-Cong Lv, Ya-Jun Jiang, Jie Liu, Wei-Ling Guo, Zhi-Bin Liu, Wen Zhang, Ping-Fan Rao, Li Ni
Denaturing gradient gel electrophoresis (DGGE) has become a widely used tool to examine microbial community structure. However, when DGGE is applied to evaluate the fungal community of traditional fermentation starters, the choice of hypervariable ribosomal RNA gene regions is still controversial. In the current study, several previously published fungal PCR primer sets were compared and evaluated using PCR-DGGE, with the purpose of screening a suitable primer set to study the fungal community of traditional fermentation starters for Hong Qu glutinous rice wine...
May 15, 2017: International Journal of Food Microbiology
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