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https://www.readbyqxmd.com/read/28100314/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-rapid-identification-of-fungal-rhinosinusitis-pathogens
#1
Xinxin Lu, Yanfei Huang, Mingxin Zhang, Min Zhu, Mei Wang, Yufeng Sun, Haitong Gu, Jingjing Cao, Xue Li, Shaoya Zhang, Jinglin Wang
BACKGROUND: filamentous fungi are the most important pathogens causing fungal rhinosinusitis (FRS). Current laboratory diagnosis of FRS pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time-consuming and expertise-dependent. Although matrix -assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been employed to identify various fungi, its performance for the identification of FRS fungi is less clear...
January 17, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28053593/evaluation-of-aerial-microbial-pollutants-in-al-haram-al-nabawi-during-pilgrimage-of-2013
#2
Kholoud M Alananbeh, Nahla Boquellah, Nadia Al Kaff, Majid Al Ahmadi
Al-Madinah Al-Munawwarah is the second holiest site in Islam. The possibility of new emerging microbes is valid due to the increased number of pilgrims. The objectives of the current study were to estimate the numbers of fungi and bacteria inside and outside Al-Haram Al-Nabawi and to find whether new bacterial and fungal species have emerged compared to previous studies. Air samples were collected twice a day from 12 spots and four directions during the pilgrim year of 2013 for four consecutive weeks by using the sedimentation method...
January 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28053592/extracellular-biosynthesis-of-silver-nanoparticles-using-rhizopus-stolonifer
#3
Khalid AbdelRahim, Sabry Younis Mahmoud, Ahmed Mohamed Ali, Khalid Salmeen Almaary, Abd El-Zaher M A Mustafa, Sherif Moussa Husseiny
Synthesis of silver nanoparticles (AgNPs) has become a necessary field of applied science. Biological method for synthesis of AgNPs by Rhizopus stolonifer aqueous mycelial extract was used. The AgNPs were identified by UV-visible spectrometry, X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectrometry (FT-IR). The presence of surface plasmon band around 420 nm indicates AgNPs formation. The characteristic of the AgNPs within the face-centered cubic (fcc) structure are indicated by the peaks of the X-ray diffraction (XRD) pattern corresponding to (1 1 1), (2 0 0) and (2 2 0) planes...
January 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28017541/production-of-flavor-compounds-from-olive-mill-waste-by-rhizopus-oryzae-and-candida-tropicalis
#4
Onur Guneser, Asli Demirkol, Yonca Karagul Yuceer, Sine Ozmen Togay, Muge Isleten Hosoglu, Murat Elibol
The purpose of this study was to investigate the production of flavor compounds from olive mill waste by microbial fermentation of Rhizopus oryzae and Candida tropicalis. Olive mill waste fermentations were performed in shake and bioreactor cultures. Production of flavor compounds from olive mill waste was followed by Gas Chromatography-Mass spectrometry, Gas chromatography- olfactometry and Spectrum Sensory Analysis(®). As a result, 1.73-log and 3.23-log cfu/mL increases were observed in the microbial populations of R...
November 23, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27979253/physico-chemical-characteristics-and-fungal-profile-of-four-saudi-fresh-date-phoenix-dactylifera-l-cultivars
#5
Fahad Al-Asmari, Nilesh Nirmal, Mridusmita Chaliha, David Williams, Ram Mereddy, Kinnari Shelat, Yasmina Sultanbawa
Physico-chemical and microbial analyses of four commercial fresh date cultivars (Sukari, Barhi, Khalas and Rothana) grown in Saudi Arabia were evaluated. Colorimetric assay indicated that Sukari had the highest total sugar content of 13.21g/100gFW while the lowest was in Rothana with 7.96g/100gFW. Total phenolic content (TPC) ranged from 76.74 to 122.20mgGAE/100gFW in Barhi and Rothana respectively, whereas antioxidant activity was highest in Sukari (105.99μgGAE/gFW) and lowest in Khalas (90.81μgGAE/gFW)...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27965127/the-preparation-and-characterization-of-chitin-and-chitosan-under-large-scale-submerged-fermentation-level-using-shrimp-by-products-as-substrate
#6
Hongcai Zhang, Sanyue Yun, Lingling Song, Yiwen Zhang, Yanyun Zhao
The crustacean shells of crabs and shrimps produces quantities of by-products, leading to seriously environmental pollution and human health problems during industrial processing, yet they turned into high-value useful products, such as chitin and chitosan. To prepare them under large-scale submerged fermentation level, shrimp shell powders (SSPs) was fermented by successive three-step fermentation of Serratia marcescens B742, Lactobacillus plantarum ATCC 8014 and Rhizopus japonicus M193 to extract chitin and chitosan based on previously optimal conditions...
December 10, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27960635/cationic-surfactant-modified-biosorption-of-anionic-dyes-by-dried-rhizopus-arrhizus
#7
Süheyla Pınar Kodal, Zümriye Aksu
The biosorption of an anionic dye Remazol Black B onto a filamentous, dried fungus, Rhizopus arrhizus, was studied in the presence of the cationic surfactant cetyl trimethyl ammonium bromide (CTAB), which was used as a pH shifter. Without surfactant, the maximum dye uptake capacity was achieved at pH 2.0 and was 434.0 mg dye g(-1) dried fungal biomass. Again, without surfactant, the highest dye uptake capacity, 27.8 mg dye g(-1) dried biomass, occurred at pH 8.0 calculated from the Langmuir adsorption model...
January 3, 2017: Environmental Technology
https://www.readbyqxmd.com/read/27960283/metabolism-of-quercetin-and-naringenin-by-food-grade-fungal-inoculum-rhizopus-azygosporus-yuan-et-jong-atcc-48108
#8
Gerard Bryan Gonzales, Guy Smagghe, Jens Wittevrongel, Nguyen Thai Huynh, John Van Camp, Katleen Raes
Rhizopus azygosporus Yuan et Jong (ATCC 48108), a starter culture for fermented soybean tempeh, produces β-glucosidases that cleave flavonoid glycosides into aglycones during fermentation. However, recent data suggest that fermentation of a flavonoid glycoside-rich extract with this strain did not result in the production of aglycones. Thus, in this paper, flavonoid metabolism of this strain was investigated. Incubation of flavonoid aglycones, naringenin and quercetin, with R. azygosporus resulted in the production of flavonoid glucosyl-, hydroxyl-, and sulfo-conjugated derivatives...
December 14, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27956601/lipid-metabolic-changes-in-an-early-divergent-fungus-govern-the-establishment-of-a-mutualistic-symbiosis-with-endobacteria
#9
Olga A Lastovetsky, Maria L Gaspar, Stephen J Mondo, Kurt M LaButti, Laura Sandor, Igor V Grigoriev, Susan A Henry, Teresa E Pawlowska
The recent accumulation of newly discovered fungal-bacterial mutualisms challenges the paradigm that fungi and bacteria are natural antagonists. To understand the mechanisms that govern the establishment and maintenance over evolutionary time of mutualisms between fungi and bacteria, we studied a symbiosis of the fungus Rhizopus microsporus (Mucoromycotina) and its Burkholderia endobacteria. We found that nonhost R. microsporus, as well as other mucoralean fungi, interact antagonistically with endobacteria derived from the host and are not invaded by them...
December 27, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27932434/rapidly-progressing-dual-infection-with-aspergillus-and-rhizopus-when-soil-inhabitants-become-deadly-invaders
#10
Milind Bhagat, Alwyn Rapose
We present a case report of a 61-year-old patient with acute pulmonary and cerebral infections with Aspergillus and Rhizopus. The only risk factor for invasive fungal disease was high-dose corticosteroids used to treat her chronic obstructive pulmonary disease exacerbation. She had rapid progression and succumbed to her infections within 2 weeks of diagnosis in spite of aggressive antifungal therapy and surgery. To the best of our knowledge, this is the first reported case of rapidly fatal dual infection with Aspergillus and Rhizopus Our case highlights the role of high-dose corticosteroids as a risk factor for invasive fungal disease in patients without traditional risk factors like haematological malignancies, solid organ transplantation or uncontrolled diabetes...
December 8, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27917280/antifungal-activity-of-zataria-multiflora-essential-oil-loaded-solid-lipid-nanoparticles-in-vitro-condition
#11
Mahboobeh Nasseri, Shiva Golmohammadzadeh, Hossein Arouiee, Mahmoud Reza Jaafari, Hossein Neamati
OBJECTIVES: The aim of the present study was to prepare, characterize, and evaluate solid lipid nanoparticles (SLNs) containing Zataria multiflora essential oil (ZEO). MATERIALS AND METHODS: In this study, Z. multiflora essential oil-loaded solid lipid nanoparticles (ZE-SLNs) were prepared to improve its efficiency in controlling some fungal pathogens. SLNs containing Z. multiflora essential oil were prepared by high shear homogenization and ultra sound technique...
November 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27888464/mycobiota-of-ground-red-pepper-and-their-aflatoxigenic-potential
#12
Hyeonheui Ham, Sosoo Kim, Min-Hee Kim, Soohyung Lee, Sung Kee Hong, Jae-Gee Ryu, Theresa Lee
To investigate contamination of ground red pepper with fungi and mycotoxin, we obtained 30 ground red pepper samples from 15 manufacturers in the main chili-pepper-producing areas in Korea. Fungal contamination was evaluated by spreading diluted samples on potato dextrose agar plates. The total fungi counts ranged from 0 to 7.3 × 10(3) CFU/g. In the samples, the genus Aspergillus had the highest incidence, while Paecilomyces was isolated most frequently. The next most frequent genera were Rhizopus, Penicillium, Cladosporium, and Alternaria...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/27880962/production-of-cellulases-by-rhizopus-stolonifer-from-glucose-containing-media-based-on-the-regulation-of-transcriptional-regulator-cre
#13
Yingyiing Zhang, Bin Tang, Guocheng Du
Carbon catabolite repression is a crucial regulation in microorganism, while its characteristic in Rhizopus is unclear now. We extracted a carbon regulation gene cre that encoded a carbon catabolite repressor protein (CRE) from Rhizopus stolonifer TP-02, and studied the regulation of CRE by real-time qPCR. CRE responded to the glucose in a certain range, wherein it could significantly regulate part of cellulases genes (eg, bg and cbh2) without cbh1. Compared with the responding of cre and four cellulases genes to the carboxymethylcellulose sodium (CMC) and the simple carbon source (lactose), the effect of CRE was only related to the concentration of reducing sugars...
November 23, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27866629/fungal-surface-protein-mediated-one-pot-synthesis-of-stable-and-hemocompatible-gold-nanoparticles
#14
Michael Kitching, Priyadarshani Choudhary, Saikumar Inguva, Yina Guo, Meghana Ramani, Sujoy K Das, Enrico Marsili
Despite their large secretome and wide applications in bioprocesses, fungi remain underexplored in metal nanoparticles (MNP) biosynthesis. Previous studies have shown that cell surface proteins of Rhizopus oryzae play a crucial role in biomineralization of Au(III) to produce gold nanoparticles (AuNPs). Therefore, it is hypothesized that purified cell surface protein may produce in vitro AuNPs with narrow size distribution for biomedical and biocatalytic applications. However, different protein extraction methods might affect protein stability and the AuNP biosynthesis process...
December 2016: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27866619/rhizopus-stolonifer-mediated-biosynthesis-of-biocompatible-cadmium-chalcogenide-quantum-dots
#15
P Mareeswari, J Brijitta, S Harikrishna Etti, C Meganathan, Gobi Saravanan Kaliaraj
We report an efficient method to biosynthesize biocompatible cadmium telluride and cadmium sulphide quantum dots from the fungus Rhizopus stolonifer. The suspension of the quantum dots exhibited purple and greenish-blue luminescence respectively upon UV light illumination. Photoluminescence spectroscopy, X-ray diffraction, and transmission electron microscopy confirms the formation of the quantum dots. From the photoluminescence spectrum the emission maxima is found to be 424 and 476nm respectively. The X-ray diffraction of the quantum dots matches with results reported in literature...
December 2016: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27862750/skin-lesion-in-a-patient-with-acute-myeloid-leukemia
#16
Atul Kothari, Sara C Shalin, Juan Carlos Rico Crescencio, Mary J Burgess
We present the case of a 51-year-old man with acute myeloid leukemia who developed fevers with a skin lesion following the first cycle of induction chemotherapy. Skin biopsy showed evidence of invasive fungal infection. Cultures remained negative, but polymerase chain reaction on tissue detected Rhizopus oryzae complex. The patient was started on liposomal amphotericin B and underwent surgical debridement. He was switched to posaconazole, with plans for allogeneic hematopoetic stem cell transplant in the future...
November 16, 2016: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/27848109/monitoring-of-aflatoxin-g1-b1-g2-and-b2-occurrence-in-some-samples-of-walnut
#17
Reza Habibipour, Parisa Rahimi Tamandegani, Abbas Farmany
This research was conducted to monitor the aflatoxigenic fungi and aflatoxin contamination of walnut in the Hamedan province. For this purpose, 40 samples were analyzed. Aspergillus, Alternaria, Rhizopus, Cladosporium, Fusarium, yeast, and some different bacteria were isolated from walnuts. Aspergillus is the most frequent genus. Aspergillus flavus was predominantly isolated. HPLC was used for evaluation of aflatoxin contamination of walnut samples. Aflatoxins G1 (AFG1), B1 (AFB1), G2 (AFG2), and B2 (AFB2) were produced by 20 isolates...
December 2016: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27844458/intergeneric-fusant-development-using-chitinase-preparation-of-rhizopus-stolonifer-ncim-880
#18
Kailas D Sonawane, Narayan R Dandagal, Akibjaved G Naikwadi, Piyush T Gurav, Samar V Anapat, Naiem H Nadaf, Deepak B Jadhav, Shailesh R Waghmare
Fungal chitinase have tremendous applications in biotech industries, with this approach we focused on extracellular chitinase from Rhizopus stolonifer NCIM 880 for the formation of fungal protoplasts. The maximum chitinase production reached after 24 h at 2.5% colloidal chitin concentration in presence of starch as an inducer. Chitinase was extracted efficiently at 65% cold acetone concentration and then purified by using DEAE-Cellulose column chromatography. Purified chitinase having molecular weight 22 kDa with single polypeptide chain was optimally active at pH 5...
December 2016: AMB Express
https://www.readbyqxmd.com/read/27834873/biocatalytic-synthesis-of-novel-partial-esters-of-a-bioactive-dihydroxy-4-methylcoumarin-by-rhizopus-oryzae-lipase-rol
#19
Vinod Kumar, Divya Mathur, Smriti Srivastava, Shashwat Malhotra, Neha Rana, Suraj K Singh, Brajendra K Singh, Ashok K Prasad, Anjani J Varma, Christophe Len, Ramesh C Kuhad, Rajendra K Saxena, Virinder S Parmar
Highly regioselective acylation has been observed in 7,8-dihydroxy-4-methylcoumarin (DHMC) by the lipase from Rhizopus oryzae suspended in tetrahydrofuran (THF) at 45 °C using six different acid anhydrides as acylating agents. The acylation occurred regioselectively at one of the two hydroxy groups of the coumarin moiety resulting in the formation of 8-acyloxy-7-hydroxy-4-methylcoumarins, which are important bioactive molecules for studying biotansformations in animals, and are otherwise very difficult to obtain by only chemical steps...
November 9, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27832748/invasive-mucormycosis-in-children-an-epidemiologic-study-in-european-and-non-european-countries-based-on-two-registries
#20
Zoi Dorothea Pana, Danila Seidel, Anna Skiada, Andreas H Groll, Georgios Petrikkos, Oliver A Cornely, Emmanuel Roilides
BACKGROUND: Mucormycosis has emerged as a rare but frequently fatal invasive fungal disease. Current knowledge on paediatric mucormycosis is based on case reports and small series reported over several decades. Contemporary data on a large cohort of patients is lacking. METHODS: Two large international registries (Zygomyco.net and FungiScope™) were searched for mucormycosis cases in ≤19 year-old patients. Cases enrolled between 2005 and 2014 were extracted, and dual entries in the two databases merged...
November 10, 2016: BMC Infectious Diseases
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