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https://www.readbyqxmd.com/read/29458870/rapid-screening-of-starter-cultures-for-maari-based-on-antifungal-properties
#1
Donatien Kaboré, Mérilie Gagnon, Denis Roy, Hagrétou Sawadogo-Lingani, Bréhima Diawara, Gisèle LaPointe
Forty Bacillus isolates obtained from maari (used as condiment in Burkina Faso) including 17 B. subtilis, 4 B. circulans, 7 B. pumilus and 6 B. licheniformis were investigated for use as starter cultures in maari production. The isolates were screened by PCR for the sfp gene responsible for the production of the lipopeptide biosurfactant, surfactin. The sfp gene was detected in all of the seventeen B. subtilis isolates, in 2 out of 7 B. pumilus, in 4 out of 6 B. licheniformis whereas no B. circulans was positive for the sfp gene by PCR screening...
March 2018: Microbiological Research
https://www.readbyqxmd.com/read/29456961/rhinoorbitocerebral-mucormycosis-with-maggots-in-a-neglected-diabetic-patient
#2
Vivek Kumar Pathak, Rohit Saxena, Sanjeev Awasthi, Sushil Gaur, Sunil Kumar Singh
Mucormycosis is a rare opportunistic fungal infection in immunocompromised patients, Rhizopus species are most common, sometimes mucormycosis can be life threatening we report a case of rhinoorbitocerebral mucormycosis a patient with diabetic ketoacidosis secondarily infected by maggots with altered consciousness and metabolic decompensation.
March 2018: Indian Journal of Otolaryngology and Head and Neck Surgery
https://www.readbyqxmd.com/read/29452270/high-level-extracellular-production-of-rhizopus-oryzae-lipase-in-pichia-pastoris-via-a-strategy-combining-optimization-of-gene-copy-number-with-co-expression-of-erad-related-proteins
#3
Liangcheng Jiao, Qinghua Zhou, Zhixin Xu, Li Xu, Yunjun Yan
Rhizopus oryzae lipase (ROL) is an important industrial enzyme limited in application due to its low production in native strains. Here, we used a new combined strategy to overexpress ROL in Pichia pastoris. An efficient method based on bio-brick was developed to construct a series of vectors harboring different copy numbers of ROL gene cassettes, which were then transformed into P. pastoris GS115 to generate a strain with specific copy numbers of ROL. An optimized gene-dosage recombinant strain of GS115/pAOα-5ROL 11# harboring five copies of ROL was screened, revealing production of the highest activity (2700 U/mL), which was 8-fold higher than that of the strain harboring one copy...
February 13, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29430363/optimization-of-gluco-amylase-production-from-aspergillus-spp-for-its-use-in-saccharification-of-liquefied-corn-starch
#4
Deepali Jain, Priya Katyal
Fungal gluco-amylase is required for the production of sugars from starchy substrates. Commercially available fungal gluco-amylase is quite costly which makes the process uneconomical. This study was undertaken to standardize physico-chemical parameters for optimum production of gluco-amylases from Aspergillus spp. Two fungal cultures, i.e., Aspergillus niger and Aspergillus terreus, were compared for gluco-amylase activity both under stationary and shake flask conditions. Among two fungal cultures, maximum gluco-amylase activity was shown by A...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29397319/influence-of-rice-straw-derived-dissolved-organic-matter-on-lactic-acid-fermentation-by-rhizopus-oryzae
#5
Xingxuan Chen, Xiahui Wang, Yiyun Xue, Tian-Ao Zhang, Yuhao Li, Jiajun Hu, Yiu Fai Tsang, Hongsheng Zhang, Min-Tian Gao
Rice straw can be used as carbon sources for lactic acid fermentation. However, only a small amount of lactic acid is produced even though Rhizopus oryzae can consume glucose in rice straw-derived hydrolysates. This study correlated the inhibitory effect of rice straw with rice straw-derived dissolved organic matter (DOM). Lactic acid fermentations with and without DOM were conducted to investigate the effect of DOM on lactic acid fermentation by R. oryzae. Fermentation using control medium with DOM showed a similar trend to fermentation with rice straw-derived hydrolysates, showing that DOM contained the major inhibitor of rice straw...
January 31, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29387377/mycofloral-profile-and-the-radiation-sensitivity-d10-values-of-solar-dried-and-gamma-irradiated-pleurotus-ostreatus-jacq-ex-fr-kummer-fruitbodies-stored-in-two-different-packaging-materials
#6
Nii Korley Kortei, George Tawia Odamtten, Mary Obodai, Michael Wiafe-Kwagyan
The presence of fungi in our foods poses serious health risks as some genera of fungi may produce certain mycotoxins which have carcinogenic, mutagenic, teratogenic, and immunosuppressive effect on humans and animals alike. Fruitbodies of Pleurotus ostreatus were solar dried at a moisture content of 12.5 ± 0.2% and stored in polythene and polypropylene packs, gamma irradiated at doses of 0 (control), 1, 2, 3, 4, and 5 kGy at a dose rate of 1.7 kGy/hr from a Cobalt 60 source (SLL, 515, Hungary) and stored at room temperature 28-30°C for a period of 12 months...
January 2018: Food Science & Nutrition
https://www.readbyqxmd.com/read/29368026/fungal-diversity-of-human-gut-microbiota-among-eutrophic-overweight-and-obese-individuals-based-on-aerobic-culture-dependent-approach
#7
Francis M Borges, Thaís O de Paula, Marjorie R A Sarmiento, Maycon G de Oliveira, Maria L M Pereira, Isabela V Toledo, Thiago C Nascimento, Alessandra B Ferreira-Machado, Vânia L Silva, Cláudio G Diniz
Fungi have a complex role in the intestinal tract, influencing health and disease, with dysbiosis contributing to obesity. Our objectives were to investigate fungal diversity in human gut microbiota among eutrophic, overweight, and obese. Epidemiological and nutritional information were collected from adult individuals, as well as stool samples processed for selective fungi isolation and identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (yeasts) or microculture (filamentous fungi)...
January 24, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29360535/microbial-biotransformation-of-bioactive-and-clinically-useful-steroids-and-some-salient-features-of-steroids-and-biotransformation
#8
REVIEW
Nighat Sultana
Steroids are perhaps one of the most widely used group of drugs in present day. Beside the established utilization as immunosuppressive, anti-inflammatory, anti-rheumatic, progestational, diuretic, sedative, anabolic and contraceptive agents, recent applications of steroid compounds include the treatment of some forms of cancer, osteoporosis, HIV infections and treatment of declared AIDS. Steroids isolated are often available in minute amounts. So biotransformation of natural products provides a powerful means in solving supply problems in clinical trials and marketing of the drug for obtaining natural products in bulk amounts...
January 19, 2018: Steroids
https://www.readbyqxmd.com/read/29353453/optimization-of-biodiesel-synthesis-by-esterification-using-a-fermented-solid-produced-by-rhizopus-microsporus-on-sugarcane-bagasse
#9
Vanderleia Botton, Leandro Piovan, Henry França Meier, David Alexander Mitchell, Jesús Cordova, Nadia Krieger
A fermented solid containing lipases was produced by solid-state fermentation of Rhizopus microsporus on sugarcane bagasse enriched with urea, soybean oil, and a mineral solution. The dry fermented solid produced using R. microsporus (RMFS) was used to catalyze the synthesis of alkyl-esters by esterification in a solvent-free system containing ethanol and oleic acid (as a model system) or a mixture of fatty acids obtained from the physical hydrolysis of soybean soapstock acid oil (FA-SSAO) in subcritical water...
January 20, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29326525/mucormycosis-in-a-diabetic-patient-a-case-report-with-an-insight-into-its-pathophysiology
#10
Syeda Neelam Afroze, Rajani Korlepara, Guttikonda Venkateswara Rao, Jayakiran Madala
Mucormycosis is one of the most rapidly progressing and fulminant forms of fungal infection which usually begins in the nose and paranasal sinuses following inhalation of fungal spores. It is caused by organisms of the subphylum Mucormycotina, including genera as Absidia, Mucor, Rhizomucor, and Rhizopus. The incidence of mucormycosis is approximately 1.7 cases per 1,000,000 inhabitants per year. Mucormycosis affecting the maxilla is rare because of rich blood vessel supply of maxillofacial areas although more virulent fungi such as Mucor can overcome this difficulty...
October 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/29326340/rare-cause-of-pulmonary-cavitation-in-a-75-year-old-man
#11
Jaffar Al-Sheikhli, Hussein Taqi, John Drake, Ayaaz Habib
A 75-year-old man of Asian descent presented to the acute medical unit with signs and symptoms suggestive of a community-acquired pneumonia. He had multiple comorbidities and was relatively immunocompromised as a result. Initial investigations supported the diagnosis of community-acquired pneumonia complicated by a cavitating lung lesion, and the patient was treated as per hospital guidelines. He continued to deteriorate despite appropriate therapy and developed a hydropneumothorax, requiring the insertion of a chest drain...
January 10, 2018: BMJ Case Reports
https://www.readbyqxmd.com/read/29296649/primary-cutaneous-rhizopus-folliculitis-in-an-immunocompetent-woman
#12
Vanessa E Johnson, Alan S Boyd
No abstract text is available yet for this article.
January 2018: JAAD Case Reports
https://www.readbyqxmd.com/read/29288027/activity-of-the-pterophyllins-2-and-4-against-postharvest-fruit-pathogenic-fungi-comparison-with-a-synthetic-analog-and-related-intermediates
#13
Jorgelina L Pergomet, Melina G Di Liberto, Marcos G Derita, Andrea B J Bracca, Teodoro S Kaufman
The antifungal activity of pterophyllin 2, pterophyllin 4, a 5-desmethyl analog of the latter and some of their synthetic intermediates, against three postharvest phytopathogenic fungi, was evaluated. The target fungi were Rhizopus stolonifer, Botrytis cinerea and Monilinia fructicola, which affect fruits worldwide, causing important economic losses. The tests were carried out with imazalil and carbendazim as positive controls. Minimum inhibitory concentrations and minimum fungicidal concentrations were determined, and the morphology of the colonies was examined microscopically...
December 26, 2017: Fitoterapia
https://www.readbyqxmd.com/read/29261103/calli-essential-oils-synergize-with-lawsone-against-multidrug-resistant-pathogens
#14
Sameh S M Soliman, Abrar I Alsaadi, Eman G Youssef, Gregory Khitrov, Ayman M Noreddin, Mohamed I Husseiny, Ashraf S Ibrahim
The fast development of multi-drug resistant (MDR) organisms increasingly threatens global health and well-being. Plant natural products have been known for centuries as alternative medicines that can possess pharmacological characteristics, including antimicrobial activities. The antimicrobial activities of essential oil (Calli oil) extracted from the Calligonum comosum plant by hydro-steam distillation was tested either alone or when combined with lawsone, a henna plant naphthoquinone, against MDR microbes...
December 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29260396/fungal-and-herbivore-elicitation-of-the-novel-maize-sesquiterpenoid-zealexin-a4-is-attenuated-by-elevated-co2
#15
Shawn A Christensen, Alisa Huffaker, James Sims, Charles T Hunter, Anna Block, Martha M Vaughan, Denis Willett, Maritza Romero, J Erik Mylroie, W Paul Williams, Eric A Schmelz
Chemical isolation and NMR-based structure elucidation revealed a novel keto-acidic sesquiterpenoid, termed zealexin A4 (ZA4). ZA4 is elicited by pathogens and herbivory, but attenuated by heightened levels of CO 2 . The identification of the labdane-related diterpenoids, termed kauralexins and acidic sesquiterpenoids, termed zealexins, demonstrated the existence of at least ten novel stress-inducible maize metabolites with diverse antimicrobial activity. Despite these advances, the identity of co-occurring and predictably related analytes remains largely unexplored...
December 19, 2017: Planta
https://www.readbyqxmd.com/read/29240696/metalaxyl-degradation-by-mucorales-strains-gongronella-sp-and-rhizopus-oryzae
#16
Maria Rosário Martins, Cledir Santos, Pablo Pereira, Júlio Cruz-Morais, Nelson Lima
In this study, the degradation of metalaxyl was investigated in the presence of two Mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. Fungal strains were characterised by a polyphasic approach using phylogenetic analysis of the Internal Transcribed Spacer (ITS) gene region, phenotypic characterisation by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) spectral analysis, and growth kinetics experiments...
December 14, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29225797/seasonal-clustering-of-sinopulmonary-mucormycosis-in-patients-with-hematologic-malignancies-at-a-large-comprehensive-cancer-center
#17
Shobini Sivagnanam, Dhruba J Sengupta, Daniel Hoogestraat, Rupali Jain, Zach Stednick, David N Fredricks, Paul Hendrie, Estella Whimbey, Sara T Podczervinski, Elizabeth M Krantz, Jeffrey S Duchin, Steven A Pergam
Background: Invasive Mucorales infections (IMI) lead to significant morbidity and mortality in immunocompromised hosts. The role of season and climatic conditions in case clustering of IMI remain poorly understood. Methods: Following detection of a cluster of sinopulmonary IMIs in patients with hematologic malignancies, we reviewed center-based medical records of all patients with IMIs and other invasive fungal infections (IFIs) between January of 2012 and August of 2015 to assess for case clustering in relation to seasonality...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29217228/primary-cutaneous-mucormycosis-due-to-rhizopus-arrhizus-in-an-8-year-old-girl
#18
M F Albízuri-Prado, A Sánchez-Orta, A Rodríguez-Bandera, M Feito-Rodríguez
No abstract text is available yet for this article.
December 4, 2017: Actas Dermo-sifiliográficas
https://www.readbyqxmd.com/read/29205274/production-of-cellulosic-organic-acids-via-synthetic-fungal-consortia
#19
Scott A Scholz, Ian Graves, Jeremy J Minty, Xiaoxia Nina Lin
Consolidated bioprocessing is a potential breakthrough technology for reducing costs of biochemical production from lignocellulosic biomass. Production of cellulase enzymes, saccharification of lignocellulose and conversion of the resulting sugars into a chemical of interest occur simultaneously within a single bioreactor. In this study, synthetic fungal consortia composed of the cellulolytic fungus Trichoderma reesei and the production specialist Rhizopus delemar demonstrated conversion of microcrystalline cellulose (MCC) and alkaline pre-treated corn stover to fumaric acid in a fully consolidated manner without addition of cellulase enzymes or expensive supplements such as yeast extract...
December 2, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29190379/fungal-specific-cyp51-inhibitor-vt-1598-demonstrates-in-vitro-activity-against-candida-and-cryptococcus-species-endemic-fungi-including-coccidioides-species-aspergillus-species-and-rhizopus-arrhizus
#20
Nathan P Wiederhold, Hoja P Patterson, Bich Hue Tran, Christopher M Yates, Robert J Schotzinger, Edward P Garvey
Background: Invasive fungal infections, including those caused by yeasts, moulds and endemic organisms, can be significant causes of morbidity and mortality in immunocompromised hosts, those with multiple comorbidities and occasionally immunocompetent hosts. Current antifungal agents are often limited by drug toxicities, drug interactions or the development of resistance. VT-1598 is a novel tetrazole that has greater specificity for fungal Cyp51 than currently available triazoles and thus the potential for clinically significant drug interactions is reduced...
November 27, 2017: Journal of Antimicrobial Chemotherapy
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