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https://www.readbyqxmd.com/read/28538100/enzymatic-hydrolysis-of-polyester-thin-films-at-the-nanoscale-effects-of-polyester-structure-and-enzyme-active-site-accessibility
#1
Michael Thomas Zumstein, Daniela Rechsteiner, Nicolas Roduner, Veronika Perz, Doris Ribitsch, Georg M Guebitz, Hans-Peter E Kohler, Kristopher McNeill, Michael Sander
Biodegradable polyesters have a large potential to replace persistent polymers in numerous applications and to thereby reduce the accumulation of plastics in the environment. Ester hydrolysis by extracellular carboxylesterases is considered the rate-limiting step in polyester biodegradation. In this work, we systematically investigated the effects of polyester and carboxylesterase structure on the hydrolysis of nanometer-thin polyester films using a quartz-crystal microbalance with dissipation monitoring. Hydrolyzability increased with increasing polyester-chain flexibility as evidenced from differences in the hydrolysis rates and extents of aliphatic polyesters varying in the length of their dicarboxylic acid unit and of poly(butylene adipate co-terephthalate) (PBAT) polyesters varying in their terephthalate-to-adipate ratio by Rhizopus oryzae lipase and Fusarium solani cutinase...
May 24, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28512597/documentation-of-ethnomedicinal-information-and-antimicrobial-validation-of-thespesia-populnea-used-by-yanadi-tribe-of-ganugapenta-village-chittoor-district-andhra-pradesh-india
#2
Nataru Savithramma, Pulicherla Yugandhar, Pallipati Suvarnalatha Devi, Sade Ankanna, Damai Suhrulatha, Koya Siva Prasad, Ramakrishanan Ranjani, Nagoji Nagaraju, Kummara Madhava Chetty
AIM: This study aimed to document the traditional knowledge of medicinal plants and antimicrobial validation of Thespesia populnea used by Yanadi tribe of Chittoor district, Andhra Pradesh, India. MATERIALS AND METHODS: The study was mainly focused on documentation of medicinal plants used by Yanadi tribe to treat different diseases with a standard questionnaire. These plants were cross-checked in Dr. Dukes Database and available literature to know the significance of this tribe on medicinal knowledge...
April 2017: Journal of Intercultural Ethnopharmacology
https://www.readbyqxmd.com/read/28507410/isolation-and-identification-of-fungi-associated-with-spoilt-fruits-vended-in-gwagwalada-market-abuja-nigeria
#3
Samuel Mailafia, God'spower Richard Okoh, Hamza Olatunde K Olabode, Ramatu Osanupin
AIM: Annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. This study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in Gwagwalada market, Abuja, and recommend appropriate control measure. MATERIALS AND METHODS: The study was conducted in Gwagwalada metropolis, Gwagwalada Area Council of the Federal Capital Territory, Abuja, Nigeria. A total of 100 spoilt fruits which include pawpaw (Carica papaya), orange (Citrus sinensis), tomato (Lycopersicon esculentum), pineapple (Ananas comosus), and watermelon (Citrullus vulgaris) were purchased and examined for the presence of fungal organisms using standard methods...
April 2017: Veterinary World
https://www.readbyqxmd.com/read/28500670/coupled-effects-of-salt-and-pressure-on-catalytic-ability-of-rhizopus-chinensis-lipase
#4
Gang Chen, Lu Wang, Ming Miao, Chengsheng Jia, Biao Feng
BACKGROUND: Both the high pressure and environmental factors could influence the catalytic abilities of enzymes. This work investigated coupled effects of pressure and salts on Rhizopus chinensis lipase (RCL) to provide significative information for its further applications. RESULTS: The maximum activity of RCL was observed under 200 MPa at 40 °C. The highest activity was achieved at concentrations of 0.06 ~ 0.1 mol L(-1) for tested salts. The effect of monovalent cations on RCL activity followed the Hofmeister series (K(+)  > Na(+)  > Li(+) ) at 0...
May 13, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28478895/three-phase-partitioning-and-spectroscopic-characterization-of-bioactive-constituent-from-halophilic-bacillus-subtilis-emb-m15
#5
Neerja Yadav, Munishwar Nath Gupta, Sunil K Khare
In the present study, a halophilic Bacillus subtilis subsp. spizizenii (NCBI GenBank accession number KX109607) was isolated from the Sambhar Salt Lake, Rajasthan India. This organism exhibited significance antibacterial and antifungal activity against Proteus vulgaris, Bacillus subtilis, Aspergillus niger, Rhizopus oligosporus and Penicillium chrysogenum respectively. The bioactive constituent responsible for it was extracted by three phase partitioning and purified by column chromatography. The purified compound was further characterized by FTIR-ATR, NMR and Mass spectrometry...
April 26, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28476767/moderate-expression-of-sec16-increases-protein-secretion-by-saccharomyces-cerevisiae
#6
Jichen Bao, Mingtao Huang, Dina Petranovic, Jens Nielsen
The yeast Saccharomyces cerevisiae is widely used to produce biopharmaceutical proteins. However, the limited capacity of the secretory pathway may reduce its productivity. Here, we increased the secretion of a heterologous α-amylase, a model protein used for studying the protein secretory pathway in yeast, by moderately overexpressing SEC16, which is involved in protein translocation from the endoplasmic reticulum to the Golgi. The moderate overexpression of SEC16 increased α-amylase secretion by generating more endoplasmic reticulum exit sites...
May 5, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28475645/aromatic-amino-acids-in-the-cellulose-binding-domain-of-penicillium-crustosum-endoglucanase-egl1-differentially-contribute-to-the-cellulose-affinity-of-the-enzyme
#7
Jiang-Ke Yang, Wei Xiong, Fang-Yuan Chen, Li Xu, Zheng-Gang Han
The cellulose binding domain (CBD) of cellulase binding to cellulosic materials is the initiation of a synergistic action on the enzymatic hydrolysis of the most abundant renewable biomass resources in nature. The binding of the CBD domain to cellulosic substrates generally relies on the interaction between the aromatic amino acids structurally located on the flat face of the CBD domain and the glucose rings of cellulose. In this study, we found the CBD domain of a newly cloned Penicillium crustosum endoglucanase EGL1, which was phylogenetically related to Aspergillus, Fusarium and Rhizopus, and divergent from the well-characterized Trichoderma reeseis cellulase CBD domain, contain two conserved aromatic amino acid-rich regions, Y451-Y452 and Y477-Y478-Y479, among which three amino acids Y451, Y477, and Y478 structurally sited on a flat face of this domain...
2017: PloS One
https://www.readbyqxmd.com/read/28472692/elucidation-of-pressure-induced-lid-movement-and-catalysis-behavior-of-rhizopus-chinensis-lipase
#8
Gang Chen, Jing Tang, Ming Miao, Bo Jiang, Jian Jin, Biao Feng
The changes of lid movement and catalysis behavior of Rhizopus chinensis lipase under high hydrostatic pressure treatment was studied. Molecular dynamics simulation showed that the lipase lid under pressure was partially opened at below 200MPa but got more closed at over 400MPa. The interfacial activation changed little at pressure below 400MPa but became marginal with the pressure increased to 500MPa. The lipase hydrolysis ability by high pressure treatment underwent a course of initial increasing then reducing with maximum activity obtained at 200MPa and 40°C...
May 1, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28470063/green-synthesis-of-conjugated-linoleic-acids-from-plant-oils-using-a-novel-synergistic-catalytic-system
#9
Yu Zhang, Huaxiang Gu, Hao Shi, Fei Wang, Xun Li
A novel and efficient method has been developed for converting plant oil into a specific conjugated linoleic acid (CLA) using a synergistic biocatalytic system based on immobilized Propionibacterium acnes isomerase (PAI) and Rhizopus oryzae lipase (ROL). PAI exhibited the greatest catalytic activity when immobilized on D301R anion-exchange resin under optimal conditions (PAI dosage: 12,410 U PAI/g D301R; glutaraldehyde concentration: 0.4%; reaction conditions: pH 7.0, 25 °C, 60 min). Up to 109 g/L trans-10, cis 12-CLA was obtained after incubation of 200 g/L sunflower oil with PAI (1659 U/g oil) and ROL (625 mU/g oil) at pH 7...
May 4, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28455587/comprehensive-analysis-of-fungal-diversity-and-enzyme-activity-in-nuruk-a-korean-fermenting-starter-for-acquiring-useful-fungi
#10
Emily Carroll, Tran Ngoc Trinh, Hokyoung Son, Yin-Won Lee, Jeong-Ah Seo
Nuruk is a fermenting starter that is involved in the production of alcoholic beverages, and has been used in South Korea for a very long time. To analyze the fungal diversity, we collected a total of 59 nuruk samples from several companies and persons in 2013 to 2014, and obtained 364 isolates. All of the single isolated fungi were identified, both morphologically and molecularly, based on the sequences of ribosomal RNA gene [18S, ITS1-5.8S-ITS2, and 26S (D1/D2 region)]. In 46 nuruk samples out of 59 (78%), Saccharomycopsis fibuligera, a dimorphic yeast, was most frequently isolated...
May 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28452021/biodegradation-of-thermally-treated-low-density-polyethylene-by-fungus-rhizopus-oryzae-ns-5
#11
Shraddha Awasthi, Neha Srivastava, Tripti Singh, D Tiwary, Pradeep Kumar Mishra
Polythene is considered as one of the important object used in daily life. Being versatile in nature and resistant to microbial attack, they effectively cause environmental pollution. In the present study, biodegradation of low-density polyethylene (LDPE) have been performed using fungal lab isolate Rhizopus oryzae NS5. Lab isolate fungal strain capable of adhering to LDPE surface was used for the biodegradation of LDPE. This strain was identified as Rhizopus oryzae NS5 (Accession No. KT160362). Fungal growth was observed on the surface of the polyethylene when cultured in potato dextrose broth at 30 °C and 120 rpm, for 1 month...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444587/solid-state-fermentation-ssf-diversity-of-applications-to-valorize-waste-and-biomass
#12
REVIEW
M A Lizardi-Jiménez, R Hernández-Martínez
Solid state fermentation is currently used in a range of applications including classical applications, such as enzyme or antibiotic production, recently developed products, such as bioactive compounds and organic acids, new trends regarding bioethanol and biodiesel as sources of alternative energy, and biosurfactant molecules with environmental purposes of valorising unexploited biomass. This work summarizes the diversity of applications of solid state fermentation to valorize biomass regarding alternative energy and environmental purposes...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28431008/diabetes-mellitus-as-the-major-risk-factor-for-mucormycosis-in-mexico-epidemiology-diagnosis-and-outcomes-of-reported-cases
#13
Dora E Corzo-León, Luis D Chora-Hernández, Ana P Rodríguez-Zulueta, Thomas J Walsh
Mucormycosis is an emerging infectious disease with high rates of associated mortality and morbidity. Little is known about the characteristics of mucormycosis or entomophthoromycosis occurring in Mexico. A search strategy was performed of literature published in journals found in available databases and theses published online at Universidad Nacional Autónoma de México (UNAM) library website reporting clinical cases or clinical case series of mucormycosis and entomophthoromycosis occurring in Mexico between 1982 and 2016...
April 20, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28421050/reveromycins-a-and-b-from-streptomyces-sp-3-10-antifungal-activity-against-plant-pathogenic-fungi-in-vitro-and-in-a-strawberry-food-model-system
#14
Ang Lyu, Hao Liu, Hongjie Che, Long Yang, Jing Zhang, Mingde Wu, Weidong Chen, Guoqing Li
This study was conducted to determine the antifungal activity of the metabolites from Streptomyces sp. 3-10, and to purify and identify the metabolites. Meanwhile, the taxonomic status of strain 3-10 was re-evaluated. The cultural filtrates of strain 3-10 in potato dextrose broth were extracted with ethyl acetate. The resulting crude extract at 1 and 5 μg/ml inhibited growth of 22 species in 18 genera of plant pathogenic fungi and Oomycetes, accounting for 92% of the total 24 tested species, suggesting that it has a wide antifungal spectrum...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28420334/mucormycosis-in-renal-transplant-recipients-review-of-174-reported-cases
#15
Yan Song, Jianjun Qiao, Gaffi Giovanni, Guangjun Liu, Hao Yang, Jianyong Wu, Jianghua Chen
BACKGROUND: Mucormycosis is a highly lethal fungal infection especially in immunocompromised individuals. METHODS: In order to review the epidemiology, diagnosis, and treatment of mucormycosis in renal transplant recipients we searched publications of mucormycosis cases in renal transplant recipients in PUBMED database up to December 2015. RESULTS: A total of 174 cases in renal transplant recipients were included in this review. Most of the cases (76%) were male...
April 18, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28410376/tuber-indicum-shapes-the-microbial-communities-of-ectomycorhizosphere-soil-and-ectomycorrhizae-of-an-indigenous-tree-pinus-armandii
#16
Qiang Li, Jian Zhao, Chuan Xiong, Xiaolin Li, Zuqin Chen, Ping Li, Wenli Huang
The aim of this study was to investigate the effect of an ectomycorrhizal fungus (Tuber indicum) on the diversity of microbial communities associated with an indigenous tree, Pinus armandii, and the microbial communities in the surrounding ectomycorhizosphere soil. High-throughput sequencing was used to analyze the richness of microbial communities in the roots or rhizosphere of treatments with or without ectomycorrhizae. The results indicated that the bacterial diversity of ectomycorhizosphere soil was significantly lower compared with the control soil...
2017: PloS One
https://www.readbyqxmd.com/read/28405168/the-black-evil-affecting-patients-with-diabetes-a-case-of-rhino-orbito-cerebral-mucormycosis-causing-garcin-syndrome
#17
Santhosh Narayanan, Geetha Panarkandy, Gomathy Subramaniam, Chandni Radhakrishnan, N K Thulaseedharan, Neeraj Manikath, Sreejith Ramaswamy, Suma Radhakrishnan, Danish Ekkalayil
Mucormycosis is a life-threatening infection affecting patients with diabetes. It is an angioinvasive disease often resistant to treatment with a debilitating course and high mortality. Here, we report a case of a 45 year old woman with type 2 diabetes mellitus who presented to us with history of right-sided ptosis and facial palsy, and subsequently developed loss of vision and palatal palsy. She was in diabetic ketoacidosis. Nervous system examination revealed involvement of right second, third, fourth, sixth, seventh, ninth, and tenth cranial nerves, suggestive of Garcin syndrome...
2017: Infection and Drug Resistance
https://www.readbyqxmd.com/read/28389346/essential-oil-composition-and-antifungal-activity-of-melissa-officinalis-originating-from-north-est-morocco-against-postharvest-phytopathogenic-fungi-in-apples
#18
Y El Ouadi, M Manssouri, A Bouyanzer, L Majidi, H Bendaif, H Elmsellem, M A Shariati, A Melhaoui, B Hammouti
To investigate biological control methods against post-harvest phytopathogenic fungi in apples, tests on the antifungal activity of essential oil of Melissa officinalis were carried out. The essential oil, obtained by hydrodistillation, was analyzed by gas chromatography coupled with mass spectrometry (GC-MS). Analysis of the essential oil was able to detect 88.7% of the components. The main components are P-mentha-1,2,3-triol (13.1%), P-menth-3-en-8-ol (8.8%), pulegone (8.8%), piperitynone oxide (8.4%) and 2-piperitone oxide (7...
April 4, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28382234/comparative-proteomics-of-rhizopus-delemar-atcc-20344-unravels-the-role-of-amino-acid-catabolism-in-fumarate-accumulation
#19
Dorett I Odoni, Juan A Tamayo-Ramos, Jasper Sloothaak, Ruben G A van Heck, Vitor A P Martins Dos Santos, Leo H de Graaff, Maria Suarez-Diez, Peter J Schaap
The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R...
2017: PeerJ
https://www.readbyqxmd.com/read/28353044/preparation-of-biodiesel-with-liquid-synergetic-lipases-from-rapeseed-oil-deodorizer-distillate
#20
Leping Zeng, Yaojia He, Liangcheng Jiao, Kai Li, Yunjun Yan
To reduce industrial production cost, cheap and easily available rapeseed oil deodorizer distillates were used as feedstock to prepare biodiesel in this study. As a result, liquid forms of Candida rugosa lipase and Rhizopus oryzae lipase (ROL) were functioned as new and effective catalysts with biodiesel yield of 92.63% for 30 h and 94.36% for 9 h, respectively. Furthermore, the synergetic effect between the two lipases was employed to enhance biodiesel yield with a result of 98.16% in 6 h under optimized conditions via response surface methodology...
March 29, 2017: Applied Biochemistry and Biotechnology
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