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https://www.readbyqxmd.com/read/28637248/expression-and-comparison-of-codon-optimised-aspergillus-tubingensis-amylase-variants-in-saccharomyces-cerevisiae
#1
Rosemary A Cripwell, Shaunita H Rose, Willem H van Zyl
The expression of codon optimised genes is a popular genetic engineering approach for the production of industrially relevant proteins. This study investigates and compares the expression of codon optimised and codon adapted amylase variants. The Aspergillus tubingensis raw starch hydrolysing α-amylase (amyA) and glucoamylase (glaA) encoding genes were redesigned using synonymous codons and expressed in S. cerevisiae Y294. Codon optimisation to favour S. cerevisiae codon bias resulted in a decrease in extracellular enzyme activity of 72% (30...
June 15, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28636478/pathway-and-rate-limiting-step-of-glyphosate-degradation-by-aspergillus-oryzae-a-f02
#2
Gui-Ming Fu, Yan Chen, Ru-Yi Li, Xiao-Qiang Yuan, Cheng-Mei Liu, Bin Li, Yin Wan
Aspergillus oryzae A-F02, a glyphosate-degrading fungus, was isolated from an aeration tank in a pesticide factory. The pathway and rate-limiting step of glyphosate (GP) degradation were investigated through metabolite analysis. GP, aminomethylphosphonic acid (AMPA) and methylamine were detected in the fermentation liquid of A. oryzae A-F02, whereas sarcosine and glycine were not. The pathway of GP degradation in A. oryzae A-F02 was revealed: GP was firstly degraded into AMPA, which was then degraded into methylamine...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28635676/comment-on-melanisation-of-aspergillus-terreus-is-butyrolactone-i-involved-in-the-regulation-of-both-dopa-and-dhn-types-of-pigments-in-submerged-culture-microorganisms-2017-5-22
#3
Elena Geib, Matthias Brock
A recent article by Palonen et al. describes the effect of butyrolactone I on the expression of a secondary metabolite biosynthesis gene cluster from Aspergillus terreus that shows similarities to fusarubin biosynthesis gene clusters from Fusarium species. The authors claim that two different types of pigments are formed in Aspergillus terreus conidia, whereby one pigment is termed a DOPA-type melanin and the second a DHN-type melanin. Unfortunately, the terminology of the classification of melanin-types requires revision as Asp-melanin present in A...
June 21, 2017: Microorganisms
https://www.readbyqxmd.com/read/28634799/assessment-of-heat-inactivated-marine-aspergillus-flavus-as-a-novel-biosorbent-for-removal-of-cd-ii-hg-ii-and-pb-ii-from-water
#4
Mohamed E Mahmoud, Gehan M El Zokm, Aida E M Farag, Mohamed S Abdelwahab
A novel marine fungus was isolated and classified as Aspergillus flavus strain EGY11. The heat-inactivated form of isolated Aspergillus flavus was investigated and evaluated as a new eco-friendly and highly efficient biosorbent for removal of toxic heavy metals such as Cd(II), Hg(II), and Pb(II) from aqueous solutions. The SEM morphological studies of biosorbent-loaded metal ions confirmed their direct binding on the surface of heat-inactivated Aspergillus flavus. The metal biosorption capacity values were determined and optimized by the batch technique in the presence of various experimental controlling factors such as pH, contact time, biosorbent dosage, initial metal ion concentration, and coexisting species...
June 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28634472/antifungal-activity-against-filamentous-fungi-of-ts1-a-multifunctional-toxin-from-tityus-serrulatus-scorpion-venom
#5
Welligton M Santussi, Karla C F Bordon, Ana P N Rodrigues Alves, Camila T Cologna, Suraia Said, Eliane C Arantes
Antimicrobial peptides (AMPs) are ubiquitous and multipotent components of the innate immune defense arsenal used by both prokaryotic and eukaryotic organisms. The search for new AMPs has increased in recent years, due to the growing development of microbial resistance to therapeutical drugs. In this work, we evaluate the effects of Tityus serrulatus venom (Tsv), its fractions and its major toxin Ts1, a beta-neurotoxin, on fungi growth. The fractions were obtained by ion-exchange chromatography of Tsv. The growth inhibition of 11 pathogenic and non-pathogenic filamentous fungi (Aspergillus fumigatus, A...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28634301/microbial-glycoside-hydrolases-as-antibiofilm-agents-with-cross-kingdom-activity
#6
Brendan D Snarr, Perrin Baker, Natalie C Bamford, Yukiko Sato, Hong Liu, Mélanie Lehoux, Fabrice N Gravelat, Hanna Ostapska, Shane R Baistrocchi, Robert P Cerone, Elan E Filler, Matthew R Parsek, Scott G Filler, P Lynne Howell, Donald C Sheppard
Galactosaminogalactan and Pel are cationic heteropolysaccharides produced by the opportunistic pathogens Aspergillus fumigatus and Pseudomonas aeruginosa, respectively. These exopolysaccharides both contain 1,4-linked N-acetyl-d-galactosamine and play an important role in biofilm formation by these organisms. Proteins containing glycoside hydrolase domains have recently been identified within the biosynthetic pathway of each exopolysaccharide. Recombinant hydrolase domains from these proteins (Sph3h from A...
June 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28634239/the-dual-activity-responsible-for-the-elongation-and-branching-of-%C3%AE-1-3-glucan-in-the-fungal-cell-wall
#7
Vishukumar Aimanianda, Catherine Simenel, Cecile Garnaud, Cecile Clavaud, Rui Tada, Lise Barbin, Isabelle Mouyna, Christoph Heddergott, Laura Popolo, Yoshikazu Ohya, Muriel Delepierre, Jean-Paul Latge
β-(1,3)-Glucan, the major fungal cell wall component, ramifies through β-(1,6)-glycosidic linkages, which facilitates its binding with other cell wall components contributing to proper cell wall assembly. Using Saccharomyces cerevisiae as a model, we developed a protocol to quantify β-(1,6)-branching on β-(1,3)-glucan. Permeabilized S. cerevisiae and radiolabeled substrate UDP-((14)C)glucose allowed us to determine branching kinetics. A screening aimed at identifying deletion mutants with reduced branching among them revealed only two, the bgl2Δ and gas1Δ mutants, showing 15% and 70% reductions in the branching, respectively, compared to the wild-type strain...
June 20, 2017: MBio
https://www.readbyqxmd.com/read/28631979/comparative-study-of-the-production-of-extracellular-%C3%AE-glucosidase-by-four-different-strains-of-aspergillus-using-submerged-fermentation
#8
Cristian Alarid-García, Eleazar Maximo Escamilla-Silva
Four strains of Aspergillus (Aspergillus niger CDBB-H-176, A. niger CDBB-H-175, A. niger ATCC 9642, and Aspergillus terreus CDBB-H-194) were used to produce extracellular β-glucosidase. Using an orthogonal experimental design (L9), we optimized the parameters of culture medium to maximize the activity of β-glucosidase. The optimal conditions (same for the four strains) were as follows: temperature, 30°C; pH, 6.0; orbital agitation, 200 rpm; concentration of sucrose, 0.5% (w/v). The most productive strain was A...
March 2, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28631658/a-case-of-aspergillus-tracheobronchitis-in-a-patient-with-chronic-obstructive-pulmonary-disease
#9
Ming-Li Yuan, Gang Yang, Hong-Ling Hu, Wen Yin, Wei-Jun Tan, Su Zhao
Aspergillus tracheobronchitis (AT) is a unique form of invasive pulmonary aspergillosis, which is commonly found in patients with impaired immunity. Early-stage AT presents in a nonspecific way, both clinically and radiographically, thereby delaying diagnosis and resulting in a high mortality. Owing to impaired mucociliary clearance, previous nonfungal infections, and administration of corticosteroids, among other aspects, patients with chronic obstructive pulmonary disease (COPD) are predisposed to AT, although they are mostly immunocompetent...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28631638/clinicomicrobiological-profile-of-endophthalmitis-a-10-year-experience-in-a-tertiary-care-center-in-north-india
#10
Gita Satpathy, Niranjan Nayak, Meenakshi Wadhwani, Pradeep Venkwatesh, Atul Kumar, Yograj Sharma, Vishnu Sreenivas
PURPOSE: To determine the clinicomicrobiological profile of infectious agents and their antibiotic susceptibility in different type of endophthalmitis. METHODS: A retrospective review of clinical and microbiological records from January 2001 to December 2010, was performed in 1110 patients diagnosed with different type of endophthalmitis (postoperative, posttraumatic, endogenous and post keratitis) to record the demographic details, clinical presentations; microbiological agents isolated with their antimicrobial sensitivity pattern...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28631282/biosynthesis-of-complex-indole-alkaloids-elucidation-of-the-concise-pathway-of-okaramines
#11
Chen-Yu Lai, Hsiao-Ching Lin, I-Wen Lo, Ranuka T Hewage, Yi-Chen Chen, Chien-Ting Chen, Chi-Fang Lee, Steven Lin, Man-Cheng Tang
The okaramines are a class of complex indole alkaloids isolated from Penicillium and Aspergillus species. Their potent insecticidal activity arises from selectively activating glutamate-gated chloride channels (GluCls) in invertebrates, not affecting human ligand-gated anion channels. Okaramines B (1) and D (2) contain a polycyclic skeleton, including an azocine ring and an unprecedented 2-dimethyl-3-methyl-azetidine ring. Due to their complex scaffold, okaramines have inspired many total synthesis efforts, but the enzymology of the okaramine biosynthetic pathway remains unexplored...
June 20, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28631058/expression-and-characterization-of-glucose-oxidase-from-aspergillus-niger-in-yarrowia-lipolytica
#12
Fatemeh Khadivi Derakshan, Farshad Darvishi, Mehrouz Dezfulian, Catherine Madzak
Glucose oxidase (GOX) is currently used in clinical, pharmaceutical, food and chemical industries. The aim of this study was expression and characterization of Aspergillus niger glucose oxidase gene in the yeast Yarrowia lipolytica. For the first time, the GOX gene of A. niger was successfully expressed in Y. lipolytica using a mono-integrative vector containing strong hybrid promoter and secretion signal. The highest total glucose oxidase activity was 370 U/L after 7 days of cultivation. An innovative method was used to cell wall disruption in current study, and it could be recommended to use for efficiently cell wall disruption of Y...
June 19, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28630185/in-vivo-biomarker-analysis-of-intranasally-dosed-pc945-a-novel-antifungal-triazole-on-aspergillus-fumigatus-infection-in-immunocompromised-mice
#13
Genki Kimura, Takahiro Nakaoki, Thomas Colley, Garth Rapeport, Pete Strong, Kazuhiro Ito, Yasuo Kizawa
PC945 is a novel triazole optimised for lung delivery and the objective of this study is to determine the effects of intranasally dosed PC945 on Aspergillus fumigatus infection and associated biomarkers in immunocompromised mice. PC945, posaconazole or voriconazole was treated intranasally once daily on days 0 to 3 (early intervention) or days 1 to 3 (late intervention) post-infection in temporarily neutropenic A/J mice infected intranasally with A. fumigatus, and bronchoalveolar lavage fluid (BALF) and serum were collected on day 3...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28629967/isolation-of-fungi-from-dung-of-wild-herbivores-for-application-in-bioethanol-production
#14
Rhulani Makhuvele, Ignatious Ncube, Elbert Lukas Jansen van Rensburg, Daniël Coenrad La Grange
Producing biofuels such as ethanol from non-food plant material has the potential to meet transportation fuel requirements in many African countries without impacting directly on food security. The current shortcomings in biomass processing are inefficient fermentation of plant sugars, such as xylose, especially at high temperatures, lack of fermenting microbes that are able to resist inhibitors associated with pre-treated plant material and lack of effective lignocellulolytic enzymes for complete hydrolysis of plant polysaccharides...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629346/a-hyper-thermostable-%C3%AE-amylase-from-pyrococcus-furiosus-accumulates-in-nicotiana-tabacum-as-functional-aggregates
#15
Hong Zhu, L Bruce Reynolds, Rima Menassa
BACKGROUND: Alpha amylase hydrolyzes α-bonds of polysaccharides such as starch and produces malto-oligosaccharides. Its starch saccharification applications make it an essential enzyme in the textile, food and brewing industries. Commercially available α-amylase is mostly produced from Bacillus or Aspergillus. A hyper-thermostable and Ca (2++) independent α-amylase from Pyrococcus furiosus (PFA) expressed in E.coli forms insoluble inclusion bodies and thus is not feasible for industrial applications...
June 19, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28628021/correlation-between-spirometry-dates-and-special-allergen-specific-ige-in-patients-with-bronchial-asthma-in-west-georgia
#16
R Sepiashvili, M Chikhladze, D Khachapuridze, S Gamkrelidze
The study aimed to establish correlation between airway obstruction and specific IgE specificity, managing future treatment in the patients with bronchial asthma, among the population of west Georgia. In the study have been involved 56 patients (among them 24 males and 32 females) of different ages, with diagnostic bronchial asthma (according to GINA recommendation). On the ground of the aim the study included the following steps of allegro-diagnostics: I step - Computerized spirometry by apparatus ,,SPIROLAB 3"...
May 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28626746/frequency-of-cutaneous-fungal-infections-and-azole-resistance-of-the-isolates-in-patients-with-diabetes-mellitus
#17
Omid Raiesi, Mansour Siavash, Faezeh Mohammadi, Javaher Chabavizadeh, Behzad Mahaki, Mehrnoush Maherolnaghsh, Parvin Dehghan
BACKGROUND: Diabetic patients are more susceptible to cutaneous fungal infections. The higher blood sugar levels cause increasing the cutaneous fungal infections in these patients. The main objective of this study was to find the frequency of fungal infections among cutaneous lesions of diabetic patients and to investigate azole antifungal agent susceptibility of the isolates. MATERIALS AND METHODS: In this study, type 1diabetes (n = 78) and type 2 diabetes (n = 44) comprised 47 cases (38...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/28626424/relationship-of-mycotoxins-accumulation-and-bioactive-components-variation-in-ginger-after-fungal-inoculation
#18
Zhixin Yang, Haiwei Wang, Guangyao Ying, Meihua Yang, Yujiao Nian, Jiajia Liu, Weijun Kong
Ginger has got increasing worldwide interests due to its extensive biological activities, along with high medical and edible values. But fungal contamination and mycotoxin residues have brought challenges to its quality and safety. In the present study, the relationship of content of mycotoxins accumulation and bioactive components variation in ginger after infection by toxigenic fungi were investigated for the first time to elucidate the influence of fungal contamination on the inherent quality of ginger. After being infected by Aspergillus flavus and Aspergillus carbonarius for different periods, the produced mycotoxins was determined by an immunoaffinity column clean-up based ultra-fast liquid chromatography coupled with tandem mass spectrometry, and the main bioactive components in ginger were analyzed by ultra performance liquid chromatography-photodiode array detection...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28623822/a-novel-photosensitization-treatment-for-the-inactivation-of-fungal-spores-and-cells-mediated-by-curcumin
#19
Fahad Al-Asmari, Ram Mereddy, Yasmina Sultanbawa
The global concerns regarding the emergence of fungicide-resistant strains and the impact of the excessive use of fungicidal practises on our health, food, and environment have increased, leading to a demand for alternative clean green technologies as treatments. Photosensitization is a treatment that utilises a photosensitiser, light and oxygen to cause cell damage to microorganisms. The effect of photosensitization mediated by curcumin on Aspergillus niger, Aspergillus flavus, Penicillium griseofulvum, Penicillium chrysogenum, Fusarium oxysporum, Candida albicans and Zygosaccharomyces bailii was investigated using three methods...
June 8, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28623814/synthesis-antimicrobial-antiquorum-sensing-and-antitumor-activities-of-new-benzimidazole-analogs
#20
N S El-Gohary, M I Shaaban
New benzimidazole analogs were prepared and tested for antimicrobial efficacy toward Escherichia coli ATCC 12435, Bacillus cereus UW 85, Staphylococcus aureus ATCC 29213, Candida albicans and Aspergillus fumigatus 293. Results indicated that compound 10 has potent and broad spectrum antimicrobial activity. In addition, 4b and 5c showed eminent antimicrobial efficacy toward B. cereus, S. aureus, C. albicans and A. fumigatus. Furthermore, 12 and 14 demonstrated interesting antifungal activity toward C. albicans...
June 3, 2017: European Journal of Medicinal Chemistry
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