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LC3 associated phagocytosis

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https://www.readbyqxmd.com/read/29215797/rubicon-lc3-associated-phagocytosis-and-beyond
#1
REVIEW
Sing-Wai Wong, Payel Sil, Jennifer Martinez
Rubicon (Rubcn) was initially identified as a component of the Class III PI3K complex and a negative regulator of canonical autophagy and endosomal trafficking. However, Rubicon has attracted the most notoriety because of its critical role in LC3-associated phagocytosis (LAP), a form of non-canonical autophagy that utilizes some components of the autophagy machinery to process extracellular cargo. Additionally, Rubicon has been identified as a key modulator of the inflammatory response and viral replication...
December 7, 2017: FEBS Journal
https://www.readbyqxmd.com/read/29169848/lc3-associated-phagocytosis-in-microbial-pathogenesis
#2
REVIEW
Stefan Schille, Peter Crauwels, Rebecca Bohn, Katrin Bagola, Ger van Zandbergen
Phagocytosis is essential for uptake and elimination of pathogenic microorganisms. Autophagy is a highly conserved mechanism for incorporation of cellular constituents to replenish nutrients by degradation. Recently, parts of the autophagy machinery - above all microtubule-associated protein 1 light chain 3 (LC3) - were found to be specifically recruited to phagosomal membranes resulting in phagosome-lysosome fusion and efficient degradation of internalized cargo in a process termed LC3-associated phagocytosis (LAP)...
November 20, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29163544/macrophage-autophagy-and-bacterial-infections
#3
REVIEW
Aïcha Bah, Isabelle Vergne
Autophagy is a well-conserved lysosomal degradation pathway that plays key roles in bacterial infections. One of the most studied is probably xenophagy, the selective capture and degradation of intracellular bacteria by lysosomes. However, the impact of autophagy goes beyond xenophagy and involves intensive cross-talks with other host defense mechanisms. In addition, autophagy machinery can have non-canonical functions such as LC3-associated phagocytosis. In this review, we intend to summarize the current knowledge on the many functions of autophagy proteins in cell defenses with a focus on bacteria-macrophage interaction...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29144836/autophagy-and-inflammation
#4
Yu Matsuzawa-Ishimoto, Seungmin Hwang, Ken Cadwell
The cellular degradative pathway of autophagy has a fundamental role in immunity. Here, we review the function of autophagy and autophagy proteins in inflammation. We discuss how the autophagy machinery controls the burden of infectious agents while simultaneously limiting inflammatory pathologies, which often involves processes that are distinct from conventional autophagy. Among the newly emerging processes we describe are LC3-associated phagocytosis and targeting by autophagy proteins, both of which require many of the same proteins that mediate conventional autophagy...
November 16, 2017: Annual Review of Immunology
https://www.readbyqxmd.com/read/29134671/macrophage-roles-in-the-clearance-of-apoptotic-cells-and-control-of-inflammation-in-the-prostate-gland-after-castration
#5
Juliete A F Silva, Alexandre Bruni-Cardoso, Taize M Augusto, Danilo M Damas-Souza, Guilherme O Barbosa, Sérgio L Felisbino, Dagmar R Stach-Machado, Hernandes F Carvalho
BACKGROUND: Androgen deprivation results in massive apoptosis in the prostate gland. Macrophages are actively engaged in phagocytosing epithelial cell corpses. However, it is unknown whether microtubule-associated protein 1 light chain 3 alpha (LC3)-associated phagocytosis (LAP) is involved and contribute to prevent inflammation. METHODS: Flow cytometry, RT-PCR and immunohistochemistry were used to characterize the macrophage subpopulation residing in the epithelial layer of the rat ventral prostate (VP) after castration...
November 14, 2017: Prostate
https://www.readbyqxmd.com/read/29118762/lc3-associated-phagocytosis-is-required-for-dendritic-cell-inflammatory-cytokine-response-to-gut-commensal-yeast-saccharomyces-cerevisiae
#6
Dimitra Lamprinaki, Gemma Beasy, Aleksandra Zhekova, Alexandra Wittmann, Steve James, Jo Dicks, Yoichiro Iwakura, Shinobu Saijo, Xiaomin Wang, Chung-Wai Chow, Ian Roberts, Tamas Korcsmaros, Ulrike Mayer, Thomas Wileman, Norihito Kawasaki
The human fungal microbiota known as mycobiota is increasingly recognized as a critical factor in human gut health and disease. Non-pathogenic commensal yeasts such as Saccharomyces cerevisiae promote homeostasis in the gut, whereas dysbiosis of the gut mycobiota is associated with inflammation. Glycan-binding receptors (lectins) are key host factors in host-mycobiota interaction in the gut. They are expressed on immune cells such as dendritic cells (DCs) and recognize fungal polysaccharides. This interaction is imperative to mount appropriate immune responses for immune homeostasis in the gut as well as clearance of fungal pathogens...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29102900/how-toxoplasma-and-malaria-parasites-defy-first-then-exploit-host-autophagic-and-endocytic-pathways-for-growth
#7
REVIEW
Isabelle Coppens
Infections caused by the apicomplexan parasites Plasmodium and Toxoplasma are wide-spread, life-threatening and therapeutically challenging. These pathogens are obligate intracellular microorganisms that invade mammalian cells by forming a self-made niche, the parasitophorous vacuole that is impervious to host lysosomal fusion. Shortly after invasion, a noncanonical xenophagic pathway resembling LC3-associated phagocytosis is activated by the host cell to control infections. However, Plasmodium and Toxoplasma have elaborated strategies to avoid clearance by the sentinel activities of the host autophagic system...
November 3, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29029244/elmo1-regulates-the-induction-of-autophagy-and-bacterial-clearance-during-enteric-infection
#8
Arup Sarkar, Courtney Tindle, Rama F Pranadinata, Sharon Reed, Lars Eckmann, Thaddeus S Stappenbeck, Peter B Ernst, Soumita Das
Macrophages are specialized phagocytic cells involved in clearing invading pathogens. Earlier we reported the Engulfment and Cell Motility protein 1 (ELMO1) in macrophages mediates bacterial internalization and intestinal inflammation. Here we studied the role of ELMO1 in the fate of internalized target. ELMO1 is present in the intracellular vesicles and enhanced LC3B accumulation following engulfment of Salmonella or treatment with autophagy-inducing rapamycin. ATG5 and ULK1 are involved in classical autophagy while LC3-associated phagocytosis (LAP) is ULK1-independent...
October 5, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28973896/mycobacterium-tuberculosis-is-protected-from-nadph-oxidase-and-lc3-associated-phagocytosis-by-the-lcp-protein-cpsa
#9
Stefan Köster, Sandeep Upadhyay, Pallavi Chandra, Kadamba Papavinasasundaram, Guozhe Yang, Amir Hassan, Steven J Grigsby, Ekansh Mittal, Heidi S Park, Victoria Jones, Fong-Fu Hsu, Mary Jackson, Christopher M Sassetti, Jennifer A Philips
Mycobacterium tuberculosis' success as a pathogen comes from its ability to evade degradation by macrophages. Normally macrophages clear microorganisms that activate pathogen-recognition receptors (PRRs) through a lysosomal-trafficking pathway called "LC3-associated phagocytosis" (LAP). Although Mtuberculosis activates numerous PRRs, for reasons that are poorly understood LAP does not substantially contribute to Mtuberculosis control. LAP depends upon reactive oxygen species (ROS) generated by NADPH oxidase, but Mtuberculosis fails to generate a robust oxidative response...
October 10, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28933590/rubcn-rubicon-and-egfr-regulate-lysosomal-degradative-processes-in-the-retinal-pigment-epithelium-rpe-of-the-eye
#10
Luis Muniz-Feliciano, Teresa A Doggett, Zhenqing Zhou, Thomas A Ferguson
Macroautophagy/autophagy is an intracellular stress survival and recycling system whereas phagocytosis internalizes material from the extracellular milieu; yet, both pathways utilize lysosomes for cargo degradation. Whereas autophagy occurs in all cells, phagocytosis is performed by cell types such as macrophages and the retinal pigment epithelial (RPE) cells of the eye where it is supported by the noncanonical autophagy process termed LC3-associated phagocytosis (LAP). Autophagy and LAP are distinct pathways that use many of the same mediators and must compete for cellular resources, suggesting that cells may regulate both processes under homeostatic and stress conditions...
September 21, 2017: Autophagy
https://www.readbyqxmd.com/read/28928467/cross-talk-between-mir-471-5p-and-autophagy-component-proteins-regulates-lc3-associated-phagocytosis-lap-of-apoptotic-germ-cells
#11
Subbarayalu Panneerdoss, Suryavathi Viswanadhapalli, Nourhan Abdelfattah, Benjamin C Onyeagucha, Santosh Timilsina, Tabrez A Mohammad, Yidong Chen, Michael Drake, Kristiina Vuori, T Rajendra Kumar, Manjeet K Rao
Phagocytic clearance of apoptotic germ cells by Sertoli cells is vital for germ cell development and differentiation. Here, using a tissue-specific miRNA transgenic mouse model, we show that interaction between miR-471-5p and autophagy member proteins regulates clearance of apoptotic germ cells via LC3-associated phagocytosis (LAP). Transgenic mice expressing miR-471-5p in Sertoli cells show increased germ cell apoptosis and compromised male fertility. Those effects are due to defective engulfment and impaired LAP-mediated clearance of apoptotic germ cells as miR-471-5p transgenic mice show lower levels of Dock180, LC3, Atg12, Becn1, Rab5 and Rubicon in Sertoli cells...
September 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28755428/safely-removing-cell-debris-with-lc3-associated-phagocytosis
#12
REVIEW
Gholamreza Fazeli, Ann Marie Wehman
Phagocytosis and autophagy are two distinct pathways that degrade external and internal unwanted particles. Both pathways lead to lysosomal degradation inside the cell, and over the last decade, the line between them has blurred; autophagy proteins were discovered on phagosomes engulfing foreign bacteria, leading to the proposal of LC3-associated phagocytosis (LAP). Many proteins involved in macroautophagy are used for phagosome degradation, although Atg8/LC3 family proteins only decorate the outer membrane of LC3-associated phagosomes, in contrast to both autophagosome membranes...
July 28, 2017: Biology of the Cell
https://www.readbyqxmd.com/read/28716903/autophagy-related-protein-vps34-controls-the-homeostasis-and-function-of-antigen-cross-presenting-cd8%C3%AE-dendritic-cells
#13
Vrajesh V Parekh, Sudheer K Pabbisetty, Lan Wu, Eric Sebzda, Jennifer Martinez, Jianhua Zhang, Luc Van Kaer
The class III PI3K Vacuolar protein sorting 34 (Vps34) plays a role in both canonical and noncanonical autophagy, key processes that control the presentation of antigens by dendritic cells (DCs) to naive T lymphocytes. We generated DC-specific Vps34-deficient mice to assess the contribution of Vps34 to DC functions. We found that DCs from these animals have a partially activated phenotype, spontaneously produce cytokines, and exhibit enhanced activity of the classic MHC class I and class II antigen-presentation pathways...
August 1, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28415949/%C3%AE-catenin-promotes-intracellular-bacterial-killing-via-suppression-of-pseudomonas-aeruginosa-triggered-macrophage-autophagy
#14
Qiang Fu, Kang Chen, Qian Zhu, Weijia Wang, Fuda Huang, Lishao Miao, Xinger Wu
Objective To investigate β-catenin-mediated bacterial elimination during Pseudomonas aeruginosa infection of macrophage-like RAW264.7 cells. Methods Cell viability and catenin beta 1 ( CTNNB1) expression in RAW264.7 cells following P. aeruginosa infection versus uninfected cells, were detected by cell counting kit-8 assay and β-catenin Western blots. RAW264.7 cells with CTNNB1 overexpression were established with β-catenin lentivirus using flow cytometry and clonogenic limiting dilution assays. Bacterial killing was measured by plate counts; phagocytosis and nitric oxide (NO) were measured by flow cytometry; and reactive oxygen species (ROS) were measured using Griess reaction...
April 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28413119/the-two-dictyostelium-discoideum-autophagy-8-proteins-have-distinct-autophagic-functions
#15
Susanne Meßling, Jan Matthias, Qiuhong Xiong, Sarah Fischer, Ludwig Eichinger
Autophagy is a highly conserved cellular degradation pathway which is crucial for various cellular processes. The autophagic process is subdivided in the initiation, autophagosome maturation and lysosomal degradation phases and involves more than forty core and accessory autophagy-related (ATG) proteins. Autophagy 8 (ATG8, in mammals LC3) is a well-established marker of autophagy and is linked to the autophagic membrane from initiation until fusion with the lysosome. We generated single and double knock-out mutants of the two Dictyostelium paralogues, ATG8a and ATG8b, as well as strains that expressed RFP-ATG8a and/or GFP-ATG8b, RFP-ATG8b, RFP-GFP-ATG8a or RFP-GFP-ATG8b in different knock-out mutants...
April 4, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28358370/the-role-of-autophagy-in-asparaginase-induced-immune-suppression-of-macrophages
#16
Ping Song, Ziyu Wang, Xuyao Zhang, Jiajun Fan, Yubin Li, Qicheng Chen, Shaofei Wang, Peipei Liu, Jingyun Luan, Li Ye, Dianwen Ju
Erwinia asparaginase, a bacteria-derived enzyme drug, has been used in the treatment of various cancers, especially acute lymphoblastic leukemia (ALL). One of the most significant side effects associated with asparaginase administration is immune suppression, which limits its application in clinic. Macrophages are phagocytic immune cells and have a central role in inflammation and host defense. We reported here that asparaginase disturbed the function of macrophages including phagocytosis, proliferation, ROS and nitric oxide secretion, interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) secretion, and major histocompatibility complex II (MHC-II) molecule expression, thus induced immune suppression in interferon-γ and lipopolysaccharide-stimulated macrophages...
March 30, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28317932/bacterial-secretion-system-skews-the-fate-of-legionella-containing-vacuoles-towards-lc3-associated-phagocytosis
#17
Andree Hubber, Tomoko Kubori, Cevayir Coban, Takeshi Matsuzawa, Michinaga Ogawa, Tsuyoshi Kawabata, Tamotsu Yoshimori, Hiroki Nagai
The evolutionarily conserved processes of endosome-lysosome maturation and macroautophagy are established mechanisms that limit survival of intracellular bacteria. Similarly, another emerging mechanism is LC3-associated phagocytosis (LAP). Here we report that an intracellular vacuolar pathogen, Legionella dumoffii, is specifically targeted by LAP over classical endocytic maturation and macroautophagy pathways. Upon infection, the majority of L. dumoffii resides in ER-like vacuoles and replicate within this niche, which involves inhibition of classical endosomal maturation...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28253971/the-use-of-dq-bsa-to-monitor-the-turnover-of-autophagy-associated-cargo
#18
L S Frost, A Dhingra, J Reyes-Reveles, K Boesze-Battaglia
There is increasing evidence documenting the critical role played by autophagic and autophagy-associated processes in maintaining cell homeostasis and overall systemic health. Autophagy is considered a degradative as well as a recycling pathway that relies on encapsulated intracellular components trafficking to and fusing with degradative compartments, including lysosomes. In this chapter, we describe the use of DQ™-BSA to study autophagosome-lysosome fusion as well as a means by which to analyze hybrid autophagic pathways...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28166684/pathogenic-mechanisms-of-ige-mediated-inflammation-in-self-destructive-autoimmune-responses
#19
Rachel Ettinger, Jodi L Karnell, Jill Henault, Santosh K Panda, Jeffrey M Riggs, Roland Kolbeck, Miguel A Sanjuan
Autoantibodies of the IgG subclass are pathogenic in a number of autoimmune disorders such as systemic lupus erythomatosus. The presence of circulating IgE autoantibodies in autoimmune patients has also been known for almost 40 years. Despite their role in allergies, IgE autoantibodies are not associated with a higher rate of atopy in these patients. However, recently they have been recognized as active drivers of autoimmunity through mechanisms involving the secretion of Type I interferons by plasmacytoid dendritic cells (pDC), the recruitment of basophils to lymph nodes, and the activation of adaptive immune responses through B and T cells...
February 2017: Autoimmunity
https://www.readbyqxmd.com/read/27978429/dapk1-keeps-the-peace-in-antifungal-inflammation
#20
Tonia Akoumianaki, Georgios Chamilos
Unabated inflammation and impaired antifungal immunity underlie genetic defects in NOX-2-dependent activation of LC3-associated phagocytosis (LAP). In this issue of Cell Host & Microbe, Oikonomou et al. (2016) identify a molecular link between IFN-γ/DAPK1 signaling, the proteosomal degradation pathway, and LAP that is critical for dampening Aspergillus-triggered immunopathology.
December 14, 2016: Cell Host & Microbe
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