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https://www.readbyqxmd.com/read/28330330/a-combined-chloroplast-atpb-rbcl-and-trnl-f-phylogeny-unveils-the-ancestry-of-balsams-impatiens-spp-in-the-western-ghats-of-india
#1
P P Shajitha, N R Dhanesh, P J Ebin, Joseph Laly, Devassy Aneesha, John Reshma, Jomy Augustine, Mathew Linu
Only a few Impatiens spp. from South India (one of the five centers of diversity for Impatiens species) were included in the published datum of molecular phylogeny of the family Balsaminaceae. The present investigation is a novel attempt to reveal the phylogenetic association of Impatiens species of South India, by placing them in the global phylogeny of Impatiens based on a combined analysis of two chloroplast genes. Thirty species of genus Impatiens were collected from different locations of South India. Total genomic DNA was extracted from fresh plant leaf, and polymerase chain reaction was carried out using atpB-rbcL and trnL-F intergenic spacer-specific forward and reverse primers...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330288/biofabrication-and-spectral-characterization-of-silver-nanoparticles-and-their-cytotoxic-studies-on-human-cd34-ve-stem-cells
#2
Venkata S Kotakadi, Susmila Aparna Gaddam, Sucharitha K Venkata, P V G K Sarma, D V R Sai Gopal
Biosynthesis of plant-mediated silver nanoparticles is gaining significant importance due to environmentally safe 'green method' and it is an efficient alternative method. In the present study, silver nanoparticles were synthesized by using root extract of Glycyrrhiza glabra an important medicinal plant. The AgNPs are characterized by spectral analysis; the surface plasmon resonance (SPR) peak of AgNPs showed maximum absorption at 445 nm. Fourier-transform infrared spectroscopy (FT-IR) data show that the O-H hydroxyl groups, carboxylic acids, ester and ether groups and C-O stretching of alcohols have been utilized in the formation of AgNPs...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330287/transformation-of-lactuca-sativa-l-with-rol-c-gene-results-in-increased-antioxidant-potential-and-enhanced-analgesic-anti-inflammatory-and-antidepressant-activities-in-vivo
#3
Hammad Ismail, Erum Dilshad, Mohammad Tahir Waheed, Moniba Sajid, Waqas Khan Kayani, Bushra Mirza
Lettuce is an important edible crop which possesses various medicinal properties. In this study Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene. Transgene integration and expression was confirmed through PCR and semiquantitative RT-PCR. The transformed extracts were evaluated for their in vitro antioxidant and in vivo analgesic, anti-inflammatory and antidepressant activities in rats. The transformed plants showed 53-98 % increase in total phenolic and 45-58 % increase in total flavonoid contents compared with untransformed plants...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330284/in-vitro-evaluation-of-anti-proliferative-anti-inflammatory-and-pro-apoptotic-activities-of-the-methanolic-extracts-of-andrographis-nallamalayana-ellis-on-a375-and-b16f10-melanoma-cell-lines
#4
Guroji Purushotham, Yerukala Padma, Yusuf Nabiha, R R Venkata Raju
Natural plant products have been widely used in controlling cancer with fewer or no side effects and the use of plant extracts as complementary to synthetic medicine is gaining increased popularity. Members of Andrographis plants possess important medicinal properties. In the present study, anti-cancerous properties of Andrographis nallamalayana (AN) were tested on A375 and B16F10 skin melanoma cancer cell lines. The leaf extracts of AN significantly reduced the cell viability and cell survival of skin cancer cell lines, achieved by MTT assay and clonogenic assays, respectively...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330281/evaluation-of-bioactive-secondary-metabolites-from-endophytic-fungus-pestalotiopsis-neglecta-bab-5510-isolated-from-leaves-of-cupressus-torulosa-d-don
#5
Deeksha Sharma, Avijit Pramanik, Pavan Kumar Agrawal
Six endophytic fungi were isolated from Cupressus torulosa D.Don and identified phenotypically and genotypically. The fungal cultures were further grown and the culture was extracted by two organic solvents methanol and ethyl acetate. The screening was carried out using the agar well diffusion method against human pathogen such as Escherichia coli, Salmonella typhimurium, Bacillus subtilis and Staphylococcus aureus. Isolated strain of Pestalotiopsis sp. was showing prominent antibacterial activity. The crude methanol and ethyl acetate extract of Pestalotiopsis sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330254/enhanced-production-of-an-anti-malarial-compound-artesunate-by-hairy-root-cultures-and-phytochemical-analysis-of-artemisia-pallens-wall
#6
Zarna Pala, Vishnu Shukla, Anshu Alok, Subhash Kudale, Neetin Desai
Artemisinin and its derivatives are still one of the most effective drugs for the treatment of malaria. Artemisia pallens commonly known as Dhavanam, is an aromatic herb belonging to the family Asteraceae. Increasing the artemisinin content of A. pallens by genetic engineering would improve the availability of this much needed drug. In the present study, Agrobacterium rhizogenes (strain NCIM 5140) mediated genetic transformation of Artemisia pallens were carried out for hairy root induction. The effect of different media (Half MS, MS, MS along with BAP 0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330226/anti-oxidant-and-anti-microbial-properties-of-some-ethno-therapeutically-important-medicinal-plants-of-indian-himalayan-region
#7
Sandeep Rawat, Arun K Jugran, Amit Bahukhandi, Asutosh Bahuguna, Indra D Bhatt, Ranbeer S Rawal, Uppeandra Dhar
Therapeutic potential of medicinal plants as a source of noble natural anti-oxidants and anti-microbial agents has been well recognised all across the globe. In this study, phenolic compounds, in vitro anti-oxidant activity and anti-microbial properties have been investigated in five Himalayan medicinal plants, (e.g., Acorus calamus, Habenaria intermedia, Hedychium spicatum, Roscoea procera and Valeriana jatamansi) in different solvent systems. R. procera exhibited significantly (p < 0.05) higher phenolics; while H...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330205/dna-record-of-some-traditional-small-millet-landraces-in-india-and-nepal
#8
Subramanyam Ragupathy, Shanmughanandhan Dhivya, Kirit Patel, Abiran Sritharan, Kathirvelu Sambandan, Hom Gartaula, Ramalingam Sathishkumar, Kamal Khadka, Balasubramanian C Nirmala, A Nirmala Kumari, Steven G Newmaster
Despite the extensive use of small millet landraces as an important source of nutrition for people living in semi-arid regions, they are presently marginalized and their diversity and distribution are threatened at a global scale. Local farmers have developed ancient breeding programs entrenched in traditional knowledge (TK) that has sustained rural cultures for thousands of years. The convention on biological diversity seeks fair and equitable sharing of genetic resources arising from local knowledge and requires signatory nations to provide appropriate policy and legal framework to farmers' rights over plant genetic resources and associated TK...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330170/an-efficient-and-cost-effective-method-of-rna-extraction-from-mucilage-phenol-and-secondary-metabolite-rich-bark-tissue-of-tossa-jute-c-olitorius-l-actively-developing-phloem-fiber
#9
S B Choudhary, M Kumar, I Chowdhury, R K Singh, S P Pandey, H K Sharma, P G Karmakar
Tossa jute is an important natural fiber crop of Southeast Asian countries including India, Bangladesh, China, Thailand, Myanmar etc. Traditional industrial application of jute fiber is limited to the packaging products like hessians, sacks, etc. and the fiber found unsuitable for textile industries largely due to significantly high lignin content. Therefore, understanding genetic factors underlying lignin biosynthesis in tossa jute holds promise for jute based product diversification. The major limiting factor in undertaking such study is unavailability of efficient protocol for RNA extraction at secondary growth active stage of tossa jute...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330131/an-efficient-protocol-for-isolation-of-inhibitor-free-nucleic-acids-even-from-recalcitrant-plants
#10
Mohammad Hossein Rezadoost, Mojtaba Kordrostami, Hassan Hassani Kumleh
For fast and easy isolation of inhibitor-free genomic DNA even from the toughest plant leaf samples, including those high in polyphenols and polysaccharides, a protocol has been developed. To prevent the solubility of polysaccharides in the DNA extract, high salt concentration (1.4 M) was used in the extraction buffer. Polyvinylpyrrolidone (PVP) was used for the removal of polyphenols as polymerase chain reaction (PCR) inhibitors. Proteins like various enzymes were degraded by proteinase K and removed by centrifugation from plant extracts during the isolation process resulting in pure DNA and RNA ready to use in downstream applications including PCR, quantitative polymerase chain reaction (qPCR), ligation, restriction and sequencing...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330130/isolation-and-characterization-of-bacterial-endophytes-of-curcuma-longa-l
#11
Ajay Kumar, Ritu Singh, Akhilesh Yadav, D D Giri, P K Singh, Kapil D Pandey
Fourteen endophytic bacterial isolates were isolated from the rhizome of Curcuma longa L. were characterized on the basis of morphology, biochemical characteristics and 16S rRNA gene sequence analysis. The isolates were identified to six strains namely Bacillus cereus (ECL1), Bacillus thuringiensis (ECL2), Bacillus sp. (ECL3), Bacillus pumilis (ECL4), Pseudomonas putida (ECL5), and Clavibacter michiganensis (ECL6). All the strains produced IAA and solubilized phosphate and only two strains produced siderophore (ECL3 and ECL5) during plant growth promoting trait analysis...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330117/microbial-pectinases-an-ecofriendly-tool-of-nature-for-industries
#12
REVIEW
G Garg, A Singh, A Kaur, R Singh, J Kaur, R Mahajan
Pectinases are the growing enzymes of biotechnological sector, showing gradual increase in their market. They hold a leading position among the commercially produced industrial enzymes. These enzymes are ecofriendly tool of nature that are being used extensively in various industries like wine industry; food industry; paper industry for bleaching of pulp and waste paper recycling; in the processing of fruit-vegetables, tea-coffee, animal feed; extraction of vegetable oil and scouring of plant fibres. Moreover, enzymatic catalysis is preferred over other chemical methods, since it is more specific, less aggressive and saves energy...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329437/phenolic-fractions-from-muscadine-grape-noble-pomace-can-inhibit-breast-cancer-cell-mda-mb-231-better-than-those-from-european-grape-cabernet-sauvignon-and-induce-s-phase-arrest-and-apoptosis
#13
Jianming Luo, Zheng Wei, Shengyu Zhang, Xichun Peng, Yu Huang, Yali Zhang, Jiang Lu
Tons of grape pomace which still contained a rich amount of plant polyphenols, is discarded after winemaking. Plant polyphenols have multi-functional activities for human body. In this study, polyphenols of pomaces from Muscadinia rotundifolia "Noble" and Vitis vinifera "Cabernet Sauvignon" were extracted and fractionated, and then they were analyzed with LC-MS and the inhibitory effects on breast cancer cells were compared. The inhibition on MDA-MB-231 cells of fractions from "Noble" was further evaluated...
March 22, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28327824/screening-of-wild-fruit-trees-with-gastroprotective-activity-in-different-experimental-models
#14
Luciane Angela Nottar Nesello, Adriana Campos, Roseane Leandra da Rosa, Sérgio Faloni de Andrade, Valdir Cechinel
BACKGROUND: Given the increase of people with gastrointestinal disorders, the search for alternative treatments with fewer side effects is vital, as well as the demand for food or plants that can help protect the stomach. OBJECTIVE: The aim of this study was to evaluate the gastroprotective action of the extracts of wild fruit trees of Myrcianthes pungens (guabiju); Inga vera Willd. (ingá-banana) and Marlierea tomentosa Cambess. (guarapuruna) in in vivo pharmacological models...
March 16, 2017: Arquivos de Gastroenterologia
https://www.readbyqxmd.com/read/28327509/stability-study-and-a-14-day-oral-dose-toxicity-in-rats-of-plantain-leaf-extract-plantago-lanceolata-l-syrup
#15
Kenza Mansoor, Fadi Qadan, Mathias Schmidt, Eyad Mallah, Wael Abudayyih, Khalid Matalka
Plants have been used since antiquity to treat and prevent diseases. Plantain (Plantago lanceolata L.) is traditionally used for the treatment of the common cold and associated symptoms such as cough. This study was designed to evaluate the oral toxicity of plantain leaf extract-containing syrup. In preparation of the toxicological examination and to ensure the quality of the herbal preparation, analytical methods were developed and validated, and stability testing was performed. Physicochemical and microbial quality, thin layer chromatography patterns and high performance liquid chromatography fingerprints complied with the specifications during the entire period of stability testing...
March 22, 2017: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/28327127/effects-of-vitellaria-paradoxa-c-f-gaertn-aqueous-leaf-extract-administration-on-salmonella-typhimurium-infected-rats
#16
Siméon Pierre Chegaing Fodouop, Sedric Donald Tala, Lunga Paul Keilah, Norbert Kodjio, Mefokou Didiane Yemele, Armel Herve Kamdje Nwabo, Bridget Nji-Kah, Joseph Tchoumboue, Donatien Gatsing
BACKGROUND: The present study investigates the effects of Vitellaria paradoxa crude extract administration on Salmonella typhimurium infected Wistar rats. METHODS: Rats were infected by single dose oral administration of Salmonella typhimurium (1.5×10(8) CFU). Negative control groups were infected and treated orally with distilled water (vehicle), neutral control group were not infected, while the four test groups were treated up to 18 days with 55 mg/kg, 110 mg/kg, 220 mg/kg and 440 mg/kg body weight of aqueous extract of V...
March 21, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28327126/characterization-and-mechanisms-of-anti-influenza-virus-metabolites-isolated-from-the-vietnamese-medicinal-plant-polygonum-chinense
#17
Thu Thi Tran, Meehyein Kim, Yejin Jang, Hye Won Lee, Hoa Thi Nguyen, Thanh Ngoc Nguyen, Hae Woong Park, Quang Le Dang, Jin-Cheol Kim
BACKGROUND: Polygonum chinense Linn. is a common medicinal plant in Southeast Asia and has been used in traditional medicine in Vietnam. The plant contains phytochemicals with various biological properties; however, its antiviral effect has not yet been demonstrated. This study was aimed to evaluate the anti-influenza virus activity of crude extracts of P. chinense, to characterize antiviral metabolites therefrom and to investigate their mechanisms of antiviral action. METHODS: The methanol (MeOH) extract and organic solvent layers of P...
March 21, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28326851/evaluation-of-antibacterial-activity-of-the-bark-and-leaf-extracts-of-brosimum-gaudichaudii-tr%C3%A3-cul-against-multidrug-resistant-strains
#18
Joelma da Costa Borges, Michele Cezimbra Perim, Rodrigo Orlandini de Castro, Thiago Antônio de Sousa Araújo, Tadeu José da Silva Peixoto Sobrinho, Ana Carolina Oliveira da Silva, Sandra Maria Botelho Mariano, Solange Cristina Carreiro, Maria Cristina da Silva Pranchevicius
Brosimum gaudichaudii Trécul., a plant that belongs to Moraceae family, is found throughout the Brazilian Cerrado. The antimicrobial activities of ethanolic bark and leaf extracts of B. gaudichaudii were tested against multiresistant bacteria isolated from diabetic foot infections (DFIs). Antimicrobial activity of the extracts was evaluated by agar disc diffusion (DD) and broth dilution (BD) methods. By BD method, bark (53.85, 45.83%) and leaf (42.31, 50.00%) extracts contained antimicrobial activity against both gram-negative and gram-positive bacteria...
March 22, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28326334/bactericide-immunomodulating-and-wound-healing-properties-of-transgenic-kalanchoe-pinnata-synergize-with-antimicrobial-peptide-cecropin-p1-in-vivo
#19
A A Lebedeva, N S Zakharchenko, E V Trubnikova, O A Medvedeva, T V Kuznetsova, G A Masgutova, M V Zylkova, Y I Buryanov, A S Belous
Procedure of manufacturing K. pinnata water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of K. pinnata + CecP1 exhibits favorable effect on healing of wounds infected with S...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28326125/determination-of-the-wound-healing-potentials-of-medicinal-plants-historically-used-in-ghana
#20
Sara H Freiesleben, Jens Soelberg, Nils T Nyberg, Anna K Jäger
The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice. The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
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