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https://www.readbyqxmd.com/read/28646766/gaps-in-aquatic-toxicological-studies-of-microplastics
#1
REVIEW
Ali Karami
The contamination of aquatic environments with microplastics (MPs) has spurred an unprecedented interest among scientific communities to investigate their impacts on biota. Despite the rapid growth in the number of studies on the aquatic toxicology of MPs, controversy over the fate and biological impacts of MPs is increasingly growing mainly due to the absence of standardized laboratory bioassays. Given the complex features of MPs, such as the diversity of constituent polymers, additives, shapes and sizes, as well as continuous changes in the particle buoyancy as a result of fouling and defouling processes, it is necessary to modify conventional bioassay protocols before employing them for MP toxicity testings...
June 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28646735/hyperpolarized-helium-3-magnetic-resonance-lung-imaging-of-non-sedated-infants-and-young-children-a-proof-of-concept-study
#2
Talissa A Altes, Craig H Meyer, Jaime F Mata, Deborah K Froh, Alix Paget-Brown, W Gerald Teague, Sean B Fain, Eduard E de Lange, Kai Ruppert, Martyn C Botfield, Mac A Johnson, John P Mugler
PURPOSE: To develop and evaluate a protocol for hyperpolarized helium-3 (HHe) ventilation magnetic resonance imaging (MRI) of the lungs of non-sedated infants and children. MATERIALS AND METHODS: HHe ventilation MRI was performed on seven children ≤4years old. Contiguous 2D-spiral helium-3 images were acquired sequentially with a scan time of ≤0.2s/slice. RESULTS: Motion-artifact-free, high signal-to-noise ratio (SNR) images of lung ventilation were obtained...
May 10, 2017: Clinical Imaging
https://www.readbyqxmd.com/read/28646693/antibacterial-effect-of-genetically-engineered-bacteriophage-%C3%AF-ef11-%C3%AF-fl1c-%C3%AE-36-p-nisa-on-dentin-infected-with-antibiotic-resistant-enterococcus-faecalis
#3
Justine Monnerat Tinoco, Nadia Liss, Hongming Zhang, Roni Nissan, Wanda Gordon, Eduardo Tinoco, Luciana Sassone, Roy Stevens
OBJECTIVE: Enterococcus faecalis is a gram-positive facultative anaerobic bacterium, which is present in 30-89% of teeth with postendodontic treatment failures. E. faecalis is capable of penetrating dentinal tubules and surviving as a monoculture after conventional endodontic therapy, indicating that it is resistant to commonly used endodontic disinfection protocols. Different E. faecalis strains have shown resistance to several antibiotics, and have been associated with both dental pathology and systemic infections...
June 12, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28646666/detection-of-mycobacterium-avium-subspecies-paratuberculosis-in-powdered-infant-formula-using-is900-quantitative-pcr-and-liquid-culture-media
#4
Kamal R Acharya, Navneet K Dhand, Richard J Whittington, Karren M Plain
Mycobacterium avium subspecies paratuberculosis (MAP) has been implicated in Crohn's disease in humans resulting in public concern over the presence of MAP in powdered infant formula, which could contribute towards early human exposure to MAP or MAP components. Testing of representative powdered infant formula samples using effective tests is required to provide information on contamination of infant formula with MAP, so that consumers can make informed decisions. This study aimed to test representative powdered infant formula samples for the presence of MAP using a quantitative PCR and liquid culture method...
June 8, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28646651/use-of-sequence-independent-single-primer-amplification-sispa-for-rapid-detection-identification-and-characterization-of-avian-rna-viruses
#5
Klaudia Chrzastek, Dong-Hun Lee, Diane Smith, Poonam Sharma, David L Suarez, Mary Pantin-Jackwood, Darrell R Kapczynski
Current technologies with next generation sequencing have revolutionized metagenomics analysis of clinical samples. To achieve the non-selective amplification and recovery of low abundance genetic sequences, a simplified Sequence-Independent, Single-Primer Amplification (SISPA) technique in combination with MiSeq platform was applied to target negative- and positive-sense single-stranded RNA viral sequences. This method allowed successful sequence assembly of full or near full length avian influenza virus (AIV), infectious bronchitis virus (IBV), and Newcastle disease virus (NDV) viral genome...
June 21, 2017: Virology
https://www.readbyqxmd.com/read/28646644/a-systematic-review-to-uncover-a-universal-protocol-for-accuracy-assessment-of-3-dimensional-virtually-planned-orthognathic-surgery
#6
REVIEW
Ramy M Gaber, Eman Shaheen, Bart Falter, Sebastian Araya, Constantinus Politis, Gwen R J Swennen, Reinhilde Jacobs
PURPOSE: The aim of this study was to systematically review methods used for assessing the accuracy of 3-dimensional virtually planned orthognathic surgery in an attempt to reach an objective assessment protocol that could be universally used. MATERIALS AND METHODS: A systematic review of the currently available literature, published until December 9, 2016, was conducted using PubMed as the primary search engine. We performed secondary searches using the Cochrane Database, clinical trial registries, Google Scholar, and Embase, as well as a bibliography search...
June 1, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28646642/universal-postpartum-mental-health-screening-for-parents-of-newborns-with-prenatally-diagnosed-birth-defects
#7
Joanna C M Cole, Michelle Olkkola, Haley Zarrin, Kelsey Berger, Julie S Moldenhauer
OBJECTIVE: To describe the implementation of a nurse-led project to screen parents for depression and traumatic stress in the postpartum period after visiting their newborns in the NICU. DESIGN: A standardized universal mental health postpartum screening and referral protocol was developed for parents of high-risk neonates. SETTING/LOCAL PROBLEM: The project occurred at the Garbose Family Special Delivery Unit, the first high-risk obstetrics unit serving healthy women who give birth to newborns with prenatally diagnosed fetal anomalies...
June 21, 2017: Journal of Obstetric, Gynecologic, and Neonatal Nursing: JOGNN
https://www.readbyqxmd.com/read/28646593/mri-assisted-dual-motion-correction-for-myocardial-perfusion-defect-detection-in-pet-imaging
#8
Xinhui Wang, Arman Rahmim, Jing Tang
PURPOSE: Myocardial perfusion (MP) PET imaging is a powerful tool in risk assess-assessment and stratification of patients with coronary artery disease. Involuntary organmotion degrades cardiac PET image resolution while respiratory and/or cardiac gating to freeze the motion leads to noisier reconstructed images due to reduced counts in the gated frames. In this work, we propose an MRI assisted dual motion correction method to compensate for respiratory and cardiac motion in MP PET data and study the impact of dual motion correction on MP defect detection using systematically designed experiments...
June 24, 2017: Medical Physics
https://www.readbyqxmd.com/read/28646588/duration-and-cessation-characteristics-of-heparinization-after-finger-replantation-a-retrospective-analysis-of-outcomes
#9
Johnny I Efanov, Julia Khriguian, Sophie Cassier, Elie Boghossian, Patrick G Harris, Joseph Bou-Merhi, M A Danino
BACKGROUND: To prevent postoperative thrombosis, indications for anticoagulation in finger replantation have been described, but no consensus has yet been found for cessation protocols. The aim of this study is to investigate cessation methods of intravenous anticoagulation after finger replantation. METHODS: A retrospective review of all patients treated for a finger replantation between December 2014 and July 2016 was performed. Only those who required postoperative treatment with intravenous heparin were extracted...
June 24, 2017: Microsurgery
https://www.readbyqxmd.com/read/28646533/palladium-catalyzed-fluoroarylation-of-gem-difluoroalkenes
#10
Hai-Jun Tang, Ling-Zhi Lin, Chao Feng, Teck-Peng Loh
Pd-catalyzed fluoroarylation of gem-difluoroalkenes with aryl halides is disclosed. By taking advantage of the in situ generated α-CF3-benzylsilver intermediates derived from the nucleophilic addition of silver fluoride onto gem-difluoroalkenens, the present strategy bypasses the employment of strong base, thus enabling a mild and general synthetic protocol for the ready access of non-symmetric α,α-disubstituted trifluoroethane derivatives.
June 24, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28646490/manganese-catalyzed-c-h-functionalizations-involving-an-unexpected-heteroaryl-shift
#11
Magnus Rueping
A manganese catalyzed regio- and stereoselective hydroarylation of allenes is reported. The C-H functionalization protocol offers access to various alkenylated indoles with excellent yields. Moreover, a hydroarylation/cyclization cascade involving an unexpected C-N bond cleavage and aryl shift has been developed which provides a new synthetic approach to substituted pyrroloindolones.
June 24, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28646478/newborn-screening-for-fabry-disease-in-the-north-west-of-spain
#12
Cristobal Colon, Saida Ortolano, Cristina Melcon-Crespo, Jose V Alvarez, Olalla E Lopez-Suarez, Maria L Couce, José R Fernández-Lorenzo
Fabry disease is an X-linked lysosomal storage disorder caused by the impairment of α-galactosidase A. Enzyme replacement therapy is available to treat patients, who often experience delayed diagnosis. A newborn screening for Fabry disease was performed to study the prevalence of the pathology and to evaluate the possibility to implement the test in systematic screenings. We collected 14,600 dried blood spot samples (7575 males and 7025 females) and carried out a diagnostic study by fluorometric measurement of α-galactosidase A enzymatic activity and GLA gene sequencing...
June 23, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28646448/purification-of-the-exopolysaccharide-produced-by-alteromonas-infernus-identification-of-endotoxins-and-effective-process-to-remove-them
#13
Alicia Grivaud-Le Du, Agata Zykwinska, Corinne Sinquin, Jacqueline Ratiskol, Pierre Weiss, Claire Vinatier, Jérôme Guicheux, Christine Delbarre-Ladrat, Sylvia Colliec-Jouault
Alteromonas infernus bacterium isolated from deep-sea hydrothermal vents can produce by fermentation a high molecular weight exopolysaccharide (EPS) called GY785. This EPS described as a new source of glycosaminoglycan-like molecule presents a great potential for pharmaceutical and biotechnological applications. However, this unusual EPS is secreted by a Gram-negative bacterium and can be therefore contaminated by endotoxins, in particular the lipopolysaccharides (LPS). Biochemical and chemical analyses of the LPS extracted from A...
June 23, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28646440/a-comparison-of-three-different-intravitreal-treatment-modalities-of-macular-edema-due-to-branch-retinal-vein-occlusion
#14
Havva Erdogan Kaldırım, Serpil Yazgan
PURPOSE: To compare the efficacy of intravitreal injection of ranibizumab, dexamethasone implant and aflibercept for the management of macular edema (ME) related to branch retinal vein occlusion (BRVO). METHODS: This retrospective and comparative study included 62 eyes of 62 patients with BRVO and ME. Patients received one of the following treatments: 0.5 mg ranibizumab (group 1, n = 22), 0.7 mg dexamethasone implant (group 2, n = 20) and 2 mg aflibercept (group 3, n = 20)...
June 23, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28646413/integrated-in-silico-in-vitro-identification-and-characterization-of-the-sh3-mediated-interaction-between-human-pttg-and-its-cognate-partners-in-medulloblastoma
#15
Jiangang Liu, Dapeng Wang, Yanyan Li, Hui Yao, Nan Zhang, Xuewen Zhang, Fangping Zhong, Yulun Huang
The human pituitary tumor-transforming gene is an oncogenic protein which serves as a central hub in the cellular signaling network of medulloblastoma. The protein contains two vicinal PxxP motifs at its C terminus that are potential binding sites of peptide-recognition SH3 domains. Here, a synthetic protocol that integrated in silico analysis and in vitro assay was described to identify the SH3-binding partners of pituitary tumor-transforming gene in the gene expression profile of medulloblastoma. In the procedure, a variety of structurally diverse, non-redundant SH3 domains with high gene expression in medulloblastoma were compiled, and their three-dimensional structures were either manually retrieved from the protein data bank database or computationally modeled through bioinformatics technique...
June 24, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28646357/management-of-hyperglycemia-and-diabetes-in-the-emergency-department
#16
REVIEW
Justin B Echouffo-Tcheugui, Rajesh Garg
PURPOSE OF REVIEW: Hyperglycemia in the emergency department (ED) is being recognized as a public health problem and presents a clinical challenge. This review critically summarizes available evidence on the burden, etiology, diagnosis, and practical management strategies for hyperglycemia in the ED. RECENT FINDINGS: Diabetic ketoacidosis (DKA) and hyperglycemic hyperosmolar state (HHS) are hyperglycemic emergencies that commonly present to the ED. However, the most common form of hyperglycemia in ED is associated with non-hyperglycemic medical emergencies...
August 2017: Current Diabetes Reports
https://www.readbyqxmd.com/read/28646280/bubble-removal-with-the-use-of-a-vacuum-pressure-generated-by-a-converging-diverging-nozzle
#17
Theodore Christoforidis, Carlos Ng, David T Eddington
Bubbles are an intrinsic problem in microfluidic devices and they can appear during the initial filling of the device or during operation. This report presents a generalizable technique to extract bubbles from microfluidic networks using an adjacent microfluidic negative pressure network over the entire microfluidic channel network design. We implement this technique by superimposing a network of parallel microchannels with a vacuum microfluidic channel and characterize the bubble extraction rates as a function of negative pressure applied...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28646261/hepatic-arterial-infusion-chemotherapy-for-extensive-liver-metastases-of-breast-cancer-efficacy-safety-and-prognostic-parameters
#18
Mitra Tewes, Michael Wilhelm Peis, Simon Bogner, Jens M Theysohn, Marcus Paul Reinboldt, Martin Schuler, Anja Welt
PURPOSE: Hepatic arterial infusion chemotherapy (HAIC) is an option for patients with liver-predominant metastatic breast cancer (LMBC), when no further systemic treatment is available. But systematic reports are limited. Here we conducted a retrospective analysis of LMBC patients treated at an expert center. METHODS: Individual patient data were retrieved from the clinical data base of the West German Cancer Center. Primary endpoints included hepatic response (RECIST), progression-free survival (PFS), overall survival (OS), and toxicity...
June 23, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28646255/protocol-to-perform-pressurized-blister-tests-on-thin-elastic-films
#19
François Boulogne, Sepideh Khodaparast, Christophe Poulard, Howard A Stone
This work aims to identify common challenges in the preparation of the blister test devices designed for the measurement of the energy release rate for brittle thin films and to propose easy-to-implement solutions accordingly. To this end, we provide a step-by-step guide for fabricating a blister test device comprised of thin polystyrene films adhered to glass substrates. Thin films are first transferred from donor substrates to an air-water interface, which is then used as a platform to locate them on a receiver substrate...
June 2017: European Physical Journal. E, Soft Matter
https://www.readbyqxmd.com/read/28646224/role-of-new-immunophenotypic-markers-on-prognostic-and-overall-survival-of-acute-myeloid-leukemia-a-systematic-review-and-meta-analysis
#20
A F O Costa, D L Menezes, L H S Pinheiro, A F Sandes, M A P Nunes, D P Lyra Junior, D M Schimieguel
Despite technological advances, the prognosis and survival of acute myeloid leukemia (AML) adult patients remain low, compared with other hematologic malignancies. Some antigens detected by immunophenotyping may soon play a significant role in the pathophysiologic, prognostic, and overall survival (OS) rate of AML patients. Therefore, we conducted a systematic review and meta-analysis of PubMed, Scopus, Science Direct, Web of Science, and the Cochrane Library (using PRISMA guidelines). We analyzed 11 studies and 13 antigens, detected through the immunophenotyping of 639 patients...
June 23, 2017: Scientific Reports
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