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Steve watson

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https://www.readbyqxmd.com/read/29715047/not-all-light-transmission-aggregation-assays-are-created-equal-qualitative-differences-between-light-transmission-and-96-well-plate-aggregometry
#1
Melissa V Chan, Philip D Leadbeater, Steve P Watson, Timothy D Warner
In this short article, submitted as part of the review on platelet function testing, we illustrate the quantitative and qualitative differences between classical light transmission aggregometry (LTA) and 96-well plate aggregometry. We show that responses to platelet agonists and antagonists differ depending upon the method of aggregation testing. For example, in 96-well aggregometry, responses to collagen are strongly inhibited by P2Y12 receptor antagonists while in LTA they are much less affected. Furthermore, we explore the importance of differences in the mechanical environment upon platelet aggregation...
May 1, 2018: Platelets
https://www.readbyqxmd.com/read/29695020/significant-hypo-responsiveness-to-gpvi-and-clec-2-agonists-in-pre-term-and-full-term-neonatal-platelets-and-following-immune-thrombocytopenia
#2
Alexander T Hardy, Verónica Palma-Barqueros, Stephanie K Watson, Jean-Daniel Malcor, Johannes A Eble, Elizabeth E Gardiner, José E Blanco, Rafael Guijarro-Campillo, Juan L Delgado, María L Lozano, Raúl Teruel-Montoya, Vicente Vicente, Steve P Watson, José Rivera, Francisca Ferrer-Marín
Neonatal platelets are hypo-reactive to the tyrosine kinase-linked receptor agonist collagen. Here, we have investigated whether the hypo-responsiveness is related to altered levels of glycoprotein VI (GPVI) and integrin α2β1, or to defects in downstream signalling events by comparison to platelet activation by C-type lectin-like receptor 2 (CLEC-2). GPVI and CLEC-2 activate a Src- and Syk-dependent signalling pathway upstream of phospholipase C (PLC) γ2. Phosphorylation of a conserved YxxL sequence known as a (hemi) immunotyrosine-based-activation-motif (ITAM) in both receptors is critical for Syk activation...
April 25, 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29659727/nitrite-circumvents-platelet-resistance-to-nitric-oxide-in-patients-with-heart-failure-preserved-ejection-fraction-and-chronic-atrial-fibrillation
#3
Alessandra Borgognone, Eduard Shantsila, Sophie M Worrall, Eakkapote Prompunt, Thomas Loka, Brodie L Loudon, Myriam Chimen, G Ed Rainger, Janet M Lord, Ashley Turner, Peter Nightingale, Martin Feelisch, Paulus Kirchhof, Gregory Y H Lip, Steve P Watson, Michael P Frenneaux, Melanie Madhani
Aims: Heart failure (HF) is a pro-thrombotic state. Both platelet and vascular responses to nitric oxide (NO) donors are impaired in HF patients with reduced ejection fraction (HFrEF) compared to healthy volunteers (HV) due to scavenging of NO, and possibly also reduced activity of the principal NO sensor, soluble guanylate cyclase (sGC), limiting the therapeutic potential of NO donors as anti-aggregatory agents. Previous studies have shown that nitrite inhibits platelet activation presumptively after its reduction to NO, but the mechanism(s) involved remain poorly characterized...
April 12, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29488279/origin-specific-adhesive-interactions-of-mesenchymal-stem-cells-with-platelets-influence-their-behavior-after-infusion
#4
Lozan Sheriff, Asma Alanazi, Lewis S C Ward, Carl Ward, Hafsa Munir, Julie Rayes, Mohammed Alassiri, Steve P Watson, Phil N Newsome, G E Rainger, Neena Kalia, Jon Frampton, Helen M McGettrick, Gerard B Nash
We investigated the adhesive behavior of mesenchymal stem cells (MSC) in blood, which might influence their fate when infused as therapy. Isolated human bone marrow MSC (BMMSC) or umbilical cord MSC (UCMSC) adhered efficiently from flow to the matrix proteins, collagen, or fibronectin, but did not adhere to endothelial selectins. However, when suspended in blood, BMMSC no longer adhered to collagen, while UCMSC adhered along with many aggregated platelets. Neither MSC adhered to fibronectin from flowing blood, although the fibronectin surface did become coated with a platelet monolayer...
February 28, 2018: Stem Cells
https://www.readbyqxmd.com/read/29482622/structured-approaches-to-promote-patient-and-family-engagement-in-treatment-in-acute-care-hospital-settings-protocol-for-a-systematic-scoping-review
#5
Donna Goodridge, Chrysanthus Henry, Erin Watson, Meghan McDonald, Lucia New, Elizabeth L Harrison, Murray Scharf, Erika Penz, Steve Campbell, Thomas Rotter
BACKGROUND: While effective engagement of patients and families in treatment is increasingly viewed as a priority for many healthcare systems, much remains to be learned about the nature and outcomes of approaches that seek to accomplish this goal in the acute care hospital setting. Wide variability in the implementation of practices designed to promote patient and family engagement in hospitals has been noted. Approaches aimed at promoting patient and family engagement in treatment share the over-arching goal of changing behaviors of patients, families, and healthcare providers and possibly administrators...
February 26, 2018: Systematic Reviews
https://www.readbyqxmd.com/read/29472360/immobilized-fibrinogen-activates-human-platelets-through-glycoprotein-vi
#6
Pierre H Mangin, Marie-Blanche Onselaer, Nicolas Receveur, Nicolas Le Lay, Alexander T Hardy, Clare Wilson, Ximena Sanchez, Stéphane Loyau, Arnaud Dupuis, Amir K Babar, Jeanette Lc Miller, Helen Philippou, Craig E Hughes, Andrew B Herr, Robert As Ariëns, Diego Mezzano, Martine Jandrot-Perrus, Christian Gachet, Steve P Watson
Glycoprotein VI, a major platelet activation receptor for collagen and fibrin, is considered a particularly promising, safe antithrombotic target. In this study, we show that human glycoprotein VI signals upon platelet adhesion to fibrinogen. Full spreading of human platelets on fibrinogen was abolished in platelets from glycoprotein VI- deficient patients suggesting that fibrinogen activates platelets through glycoprotein VI. While mouse platelets failed to spread on fibrinogen, human-glycoprotein VI-transgenic mouse platelets showed full spreading and increased Ca2+ signaling through the tyrosine kinase Syk...
May 2018: Haematologica
https://www.readbyqxmd.com/read/29443373/p2x1-receptors-amplify-fc%C3%AE-riia-induced-ca2-increases-and-functional-responses-in-human-platelets
#7
Zeki Ilkan, Stephanie Watson, Steve P Watson, Martyn P Mahaut-Smith
Platelets express key receptors of the innate immune system such as FcγRIIa and Toll-like receptors (TLR). P2X1 cation channels amplify the platelet responses to several major platelet stimuli, particularly glycoprotein (GP)VI and TLR2/1, whereas their contribution to Src tyrosine kinase-dependent FcγRIIa receptors remains unknown. We investigated the role of P2X1 receptors during activation of FcγRIIa in human platelets, following stimulation by cross-linking of an anti-FcγRIIa monoclonal antibody (mAb) IV...
February 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29437639/soluble-gpvi-is-elevated-in-injured-patients-shedding-is-mediated-by-fibrin-activation-of-gpvi
#8
Samantha J Montague, Céline Delierneux, Christelle Lecut, Nathalie Layios, Robert J Dinsdale, Christine S-M Lee, Natalie S Poulter, Robert K Andrews, Peter Hampson, Christopher M Wearn, Nathalie Maes, Jonathan Bishop, Amy Bamford, Chris Gardiner, Woei Ming Lee, Tariq Iqbal, Naiem Moiemen, Steve P Watson, Cécile Oury, Paul Harrison, Elizabeth E Gardiner
Soluble glycoprotein VI (sGPVI) is shed from the platelet surface and is a marker of platelet activation in thrombotic conditions. We assessed sGPVI levels together with patient and clinical parameters in acute and chronic inflammatory conditions, including patients with thermal injury and inflammatory bowel disease and patients admitted to the intensive care unit (ICU) for elective cardiac surgery, trauma, acute brain injury, or prolonged ventilation. Plasma sGPVI was measured by enzyme-linked immunosorbent assay and was elevated on day 14 after thermal injury, and was higher in patients who developed sepsis...
February 13, 2018: Blood Advances
https://www.readbyqxmd.com/read/29419432/the-contribution-of-platelet-glycoprotein-receptors-to-inflammatory-bleeding-prevention-is-stimulus-and-organ-dependent
#9
Julie Rayes, Soumaya Jadoui, Siân Lax, Angèle Gros, Surasak Wichaiyo, Véronique Ollivier, Cécile V Denis, Jerry Ware, Bernhard Nieswandt, Martine Jandrot-Perrus, Steve P Watson, Benoît Ho-Tin-Noé
"-".
February 1, 2018: Haematologica
https://www.readbyqxmd.com/read/29389674/inherited-factor-ii-deficiency-with-paradoxical-hypercoagulability-a-case-report
#10
Harold Watson, Ariel Perez, Kwabena Ayesu, Faisal Musa, Jose Sarriera, Mario Madruga, Steve J Carlan
: Congenital deficiency of factor II is a very rare autosomal recessive disorder that can result in a bleeding diathesis. Genotypically, individuals are either homozygous for a defective prothrombin gene or a compound heterozygote with different mutated prothrombin genes inherited from each parent. Phenotypically, it is characterized by either a low production of normal prothrombin or a near-normal production of dysfunctional prothrombin. Treatment is aimed at restoring normally functioning factor II circulating levels to sufficient concentration for hemostasis...
March 2018: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/29365922/cost-effectiveness-of-cascade-testing-for-familial-hypercholesterolaemia-based-on-data-from-fh-services-in-the-uk
#11
Steve Humphries, Robert Pears, Zofia Miedzybrodzka, Kate Haralambos, Moyra Cather, Melanie Watson, Marion Kerr
No abstract text is available yet for this article.
August 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29340588/antimicrobial-resistant-klebsiella-pneumoniae-carriage-and-infection-in-specialized-geriatric-care-wards-linked-to-acquisition-in-the-referring-hospital
#12
Claire L Gorrie, Mirjana Mirceta, Ryan R Wick, Louise M Judd, Kelly L Wyres, Nicholas R Thomson, Richard A Strugnell, Nigel F Pratt, Jill S Garlick, Kerrie M Watson, Peter C Hunter, Steve A McGloughlin, Denis W Spelman, Adam W J Jenney, Kathryn E Holt
Background: Klebsiella pneumoniae is a leading cause of extended-spectrum beta-lactamase (ESBL) producing hospital-associated infections, for which elderly patients are at increased risk. Methods: We conducted a 1-year prospective cohort study, in which a third of patients admitted to two geriatric wards in a specialized hospital were recruited and screened for carriage of K. pneumoniae by microbiological culture. Clinical isolates were monitored via the hospital laboratory...
January 11, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29313403/platelets-joining-the-400-club
#13
EDITORIAL
Paul Harrison, Steve Watson
No abstract text is available yet for this article.
January 2018: Platelets
https://www.readbyqxmd.com/read/29297721/mucor-circinelloides-induces-platelet-aggregation-through-integrin-%C3%AE-iib%C3%AE-3-and-fc%C3%AE-riia
#14
Harlene Ghuman, Alicia Shepherd-Roberts, Stephanie Watson, Malou Zuidscherwoude, Steve P Watson, Kerstin Voelz
Thrombosis is a hallmark of the fatal fungal infection mucormycosis. Yet, the platelet activation pathway in response to mucormycetes is unknown. In this study we determined the platelet aggregation potential of Mucor circinelloides (M. circinelloides) NRRL3631, characterized the signaling pathway facilitating aggregation in response to fungal spores, and identified the influence of the spore developmental stage upon platelet aggregation potential. Using impedance and light-transmission aggregometry, we showed that M...
January 3, 2018: Platelets
https://www.readbyqxmd.com/read/29296791/fibrin-and-d-dimer-bind-to-monomeric-gpvi
#15
Marie-Blanche Onselaer, Alexander T Hardy, Clare Wilson, Ximena Sanchez, Amir K Babar, Jeanette L C Miller, Callum N Watson, Stephanie K Watson, Arkadiusz Bonna, Helen Philippou, Andrew B Herr, Diego Mezzano, Robert A S Ariëns, Steve P Watson
Fibrin has recently been shown to activate platelets through the immunoglobulin receptor glycoprotein VI (GPVI). In the present study, we show that spreading of human platelets on fibrin is abolished in patients deficient in GPVI, confirming that fibrin activates human platelets through the immunoglobulin receptor. Using a series of proteolytic fragments, we show that D-dimer, but not the E fragment of fibrin, binds to GPVI and that immobilized D-dimer induces platelet spreading through activation of Src and Syk tyrosine kinases...
August 22, 2017: Blood Advances
https://www.readbyqxmd.com/read/29248433/physiotherapy-breathing-retraining-for-asthma-a-randomised-controlled-trial
#16
Anne Bruton, Amanda Lee, Lucy Yardley, James Raftery, Emily Arden-Close, Sarah Kirby, Shihua Zhu, Manimekalai Thiruvothiyur, Frances Webley, Lyn Taylor, Denise Gibson, Guiqing Yao, Mark Stafford-Watson, Jenny Versnel, Michael Moore, Steve George, Paul Little, Ratko Djukanovic, David Price, Ian D Pavord, Stephen T Holgate, Mike Thomas
BACKGROUND: Despite effective pharmacotherapy, asthma continues to impair quality of life for most patients. Non-pharmacological approaches, including breathing retraining, are therefore of great interest to patients. However, clinicians rarely advocate breathing retraining and access to this intervention is restricted for most patients due to the limited availability of suitable physiotherapists and poor integration of breathing retraining into standard care. We aimed to assess the effectiveness of a digital self-guided breathing retraining intervention...
January 2018: Lancet Respiratory Medicine
https://www.readbyqxmd.com/read/29208190/preventing-blood-borne-virus-infection-in-people-who-inject-drugs-in-the-uk-systematic-review-stakeholder-interviews-psychosocial-intervention-development-and-feasibility-randomised-controlled-trial
#17
Gail Gilchrist, Davina Swan, April Shaw, Ada Keding, Sarah Towers, Noel Craine, Alison Munro, Elizabeth Hughes, Steve Parrott, Noreen Mdege, John Strang, Avril Taylor, Judith Watson
BACKGROUND: Opioid substitution therapy and needle exchanges have reduced blood-borne viruses (BBVs) among people who inject drugs (PWID). Some PWID continue to share injecting equipment. OBJECTIVES: To develop an evidence-based psychosocial intervention to reduce BBV risk behaviours and increase transmission knowledge among PWID, and conduct a feasibility trial among PWID comparing the intervention with a control. DESIGN: A pragmatic, two-armed randomised controlled, open feasibility trial...
November 2017: Health Technology Assessment: HTA
https://www.readbyqxmd.com/read/29090616/mouse-podoplanin-supports-adhesion-and-aggregation-of-platelets-under-arterial-shear-a-novel-mechanism-of-haemostasis
#18
Stephanie E Lombard, Alice Y Pollitt, Craig E Hughes, Ying Di, Tom Mckinnon, Chris A O'callaghan, Steve P Watson
The podoplanin-CLEC-2 axis is critical in mice for prevention of hemorrhage in the cerebral vasculature during mid-gestation. This raises the question as to how platelets are captured by podoplanin on neuroepithelial cells in a high shear environment. In this study, we demonstrate that mouse platelets form stable aggregates on mouse podoplanin at arterial shear through a CLEC-2 and Src kinase-dependent pathway. Adhesion and aggregation are also dependent on the platelet glycoprotein (GP) receptors, integrin αIIbβ3 and GPIb, and the feedback agonists ADP and thromboxane A2 (TxA2)...
November 1, 2017: Platelets
https://www.readbyqxmd.com/read/29016980/effectiveness-and-cost-effectiveness-of-opportunistic-screening-and-stepped-care-interventions-for-older-alcohol-users-in-primary-care
#19
Simon Coulton, Martin Bland, Helen Crosby, Veronica Dale, Colin Drummond, Christine Godfrey, Eileen Kaner, Jennifer Sweetman, Ruth McGovern, Dorothy Newbury-Birch, Steve Parrott, Gillian Tober, Judith Watson, Qi Wu
Aims: To compare the clinical effectiveness and cost-effectiveness of a stepped-care intervention versus a minimal intervention for the treatment of older hazardous alcohol users in primary care. Method: Multi-centre, pragmatic RCT, set in Primary Care in UK. Patients aged ≥ 55 years scoring ≥ 8 on the Alcohol Use Disorders Identification Test were allocated either to 5-min of brief advice or to 'Stepped Care': an initial 20-min of behavioural change counselling, with Step 2 being three sessions of Motivational Enhancement Therapy and Step 3 referral to local alcohol services (progression between each Step being determined by outcomes 1 month after each Step)...
November 1, 2017: Alcohol and Alcoholism: International Journal of the Medical Council on Alcoholism
https://www.readbyqxmd.com/read/28983057/introducing-high-throughput-sequencing-into-mainstream-genetic-diagnosis-practice-in-inherited-platelet-disorders
#20
José M Bastida, María L Lozano, Rocío Benito, Kamila Janusz, Verónica Palma-Barqueros, Mónica Del Rey, Jesús M Hernández-Sánchez, Susana Riesco, Nuria Bermejo, Hermenegildo González-García, Agustín Rodriguez-Alén, Carlos Aguilar, Teresa Sevivas, María F López-Fernández, Anna E Marneth, Bert A van der Reijden, Neil V Morgan, Steve P Watson, Vicente Vicente, Jesús M Hernández-Rivas, José Rivera, José R González-Porras
Inherited platelet disorders are a heterogeneous group of rare diseases, caused by inherited defects in platelet production and/or function. Their genetic diagnosis would benefit clinical care, prognosis and preventative treatments. Until recently, this diagnosis has usually been performed via Sanger sequencing of a limited number of candidate genes. High-throughput sequencing is revolutionizing the genetic diagnosis of diseases, including bleeding disorders. We have designed a novel high-throughput sequencing platform to investigate the unknown molecular pathology in a cohort of 82 patients with inherited platelet disorders...
January 2018: Haematologica
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