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https://www.readbyqxmd.com/read/27933536/genetic-dissection-of-variation-in-hippocampal-intra-and-infrapyramidal-mossy-fibers-in-the-mouse
#1
Anna Delprato, Wim E Crusio
This chapter describes the genetic analysis of a morphometric neuroanatomic trait. We used the extended BXD family of recombinant inbred mouse strains with the intent to analyze the genetic bases of heritable differences in hippocampal neurocircuitry and to identify Quantitative Trait Loci that underlie these variations. A detailed description of a GeneNetwork analysis is provided using data for the intra- and infrapyramidal mossy fiber (IIPMF) terminal fields which are strongly correlated with spatial navigation/radial maze learning...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27933259/deformity-correction-and-extremity-lengthening-in-the-lower-leg-comparison-of-clinical-outcomes-with-two-external-surgical-procedures
#2
Eugen Reitenbach, Robert Rödl, Georg Gosheger, Björn Vogt, Frank Schiedel
OBJECTIVE: Distraction osteogenesis is a method of stimulating the growth of new bone tissue in order to lengthen the extremities or bridge resected bone defects. In addition to the now-established intramedullary procedures, two different fixator systems are in use. The present study investigated the classical Ilizarov ring fixator (IRF) and a hexapod to assess the precision of lower-leg lengthening and complications classified using the Paley criteria for problems, obstacles, and complications...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27933220/a-novel-method-for-quantitative-serial-autofluorescence-analysis-in-retinitis-pigmentosa-using-image-characteristics
#3
Jasleen K Jolly, Siegfried K Wagner, Jonathan Moules, Florian Gekeler, Andrew R Webster, Susan M Downes, Robert E MacLaren
PURPOSE: Identifying potential biomarkers for disease progression in retinitis pigmentosa (RP) is highly relevant now that gene therapy and other treatments are in clinical trial. Here we report a novel technique for analysis of short-wavelength autofluorescence (AF) imaging to quantify defined regions of AF in RP patients. METHODS: Fifty-five-degree AF images were acquired from 12 participants with RP over a 12-month period. Of these, five were identified as having a hyperfluorescent annulus...
December 2016: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/27932968/neurobiology-of-anorexia-nervosa-serotonin-dysfunctions-link-self-starvation-with-body-image-disturbances-through-an-impaired-body-memory
#4
REVIEW
Giuseppe Riva
The etiology of anorexia nervosa (AN) is still unclear, despite that it is a critical and potentially mortal illness. A recent neurobiological model considers AN as the outcome of dysfunctions in the neuronal processes related to appetite and emotionality (Kaye et al., 2009, 2013). However, this model still is not able to answer a critical question: What is behind body image disturbances (BIDs) in AN? The article starts its analysis from reviewing some of the studies exploring the effects of the serotonin systems in memory (episodic, working, and spatial) and its dysfunctions...
2016: Frontiers in Human Neuroscience
https://www.readbyqxmd.com/read/27932770/-contrast-sensitivity-analysis-about-fundus-changes-of-hypertensive-disorders-complicating-pregnancy
#5
Zhixue Wang, Wenying Wang, Fengmei Han
To observe corrected visual acuity and contrast sensitivity (CS) in patients with hypertensive disorders complicating pregnancy accompanied by fundus changes.
 Methods: Ninety-eight patients with hypertensive disorders complicating pregnancy and 50 healthy pregnant women underwent eye examination, including corrected visual acuity and fundus examination, and CS. Differences in corrected visual acuity and contrast sensitivity between the 2 groups were analyzed with two independent samples t-test analysis, while correlation between vision and contrast sensitivity in patients was evaluated by using spearman correlation test...
November 28, 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/27932615/anopheles-salivary-biomarker-to-assess-malaria-transmission-risk-along-the-thailand-myanmar-border
#6
Phubeth Ya-Umphan, Dominique Cerqueira, Daniel M Parker, Gilles Cottrell, Anne Poinsignon, Franck Remoue, Cecile Brengues, Theeraphap Chareonviriyaphap, Francois Nosten, Vincent Corbel
BACKGROUND: The modalities of malaria transmission along the Thailand-Myanmar border are poorly understood. Here we address the relevance of using a specific Anopheles salivary biomarker to measure the risk of human exposure to anopheles bites. METHODS: Serologic surveys were conducted from May 2013 to December 2014 in 4 sentinel villages. Dried blood spots (n>9,400) were collected in filter papers among all inhabitants at baseline and then every three months up to 18 months...
December 8, 2016: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27932220/risk-management-for-optimal-land-use-planning-integrating-ecosystem-services-values-a-case-study-in-changsha-middle-china
#7
Jie Liang, Minzhou Zhong, Guangming Zeng, Gaojie Chen, Shanshan Hua, Xiaodong Li, Yujie Yuan, Haipeng Wu, Xiang Gao
Land-use change has direct impact on ecosystem services and alters ecosystem services values (ESVs). Ecosystem services analysis is beneficial for land management and decisions. However, the application of ESVs for decision-making in land use decisions is scarce. In this paper, a method, integrating ESVs to balance future ecosystem-service benefit and risk, is developed to optimize investment in land for ecological conservation in land use planning. Using ecological conservation in land use planning in Changsha as an example, ESVs is regarded as the expected ecosystem-service benefit...
December 5, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27932216/arsenic-contamination-of-drinking-water-in-ireland-a-spatial-analysis-of-occurrence-and-potential-risk
#8
Ellen R McGrory, Colin Brown, Norma Bargary, Natalya Hunter Williams, Anthony Mannix, Chaosheng Zhang, Tiernan Henry, Eve Daly, Sarah Nicholas, Barbara M Petrunic, Monica Lee, Liam Morrison
The presence of arsenic in groundwater has become a global concern due to the health risks from drinking water with elevated concentrations. The Water Framework Directive (WFD) of the European Union calls for drinking water risk assessment for member states. The present study amalgamates readily available national and sub-national scale datasets on arsenic in groundwater in the Republic of Ireland. However, due to the presence of high levels of left censoring (i.e. arsenic values below an analytical detection limit) and changes in detection limits over time, the application of conventional statistical methods would inhibit the generation of meaningful results...
December 5, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27932215/the-land-use-legacy-effect-towards-a-mechanistic-understanding-of-time-lagged-water-quality-responses-to-land-use-cover
#9
Sherry L Martin, Daniel B Hayes, Anthony D Kendall, David W Hyndman
Numerous studies have linked land use/land cover (LULC) to aquatic ecosystem responses, however only a few have included the dynamics of changing LULC in their analysis. In this study, we explicitly recognize changing LULC by linking mechanistic groundwater flow and travel time models to a historical time series of LULC, creating a land-use legacy map. We then illustrate the utility of legacy maps to explore relationships between dynamic LULC and lake water chemistry. We tested two main concepts about mechanisms linking LULC and lake water chemistry: groundwater pathways are an important mechanism driving legacy effects; and, LULC over multiple spatial scales is more closely related to lake chemistry than LULC over a single spatial scale...
December 5, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27931140/phylogeography-of-the-endangered-orchid-dendrobium-moniliforme-in-east-asia-inferred-from-chloroplast-dna-sequences
#10
Meirong Ye, Wei Liu, Qingyun Xue, Beiwei Hou, Jing Luo, Xiaoyu Ding
The aim of the current study was to elucidate the phylogeographic history of Dendrobium moniliforme, an endangered orchid species, based on two chloroplast DNA (cpDNA) markers (trnC-petN and trnE-trnT). One hundred and thirty-five samples were collected from 18 natural populations of D. moniliforme covering the entire range of the Sino-Japanese Floristic Region (SJFR) of East Asia. A total of 35 distinct cpDNA haplotypes were identified in these populations, of which 23 haplotypes were each present in only one sample and thus restricted to a single population...
December 8, 2016: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/27930958/protected-areas-and-agricultural-expansion-biodiversity-conservation-versus-economic-growth-in-the-southeast-of-brazil
#11
Mayra Cristina Prado de Moraes, Kaline de Mello, Rogério Hartung Toppa
The conversion of natural ecosystems to agricultural land and urban areas plays a threat to the protected areas and the natural ecosystems conservation. The aim of this paper is to provide an analysis of the agricultural expansion and its impact on the landscape spatial and temporal patterns in a buffer zone of a protected area located in the transition zone between the Atlantic Forest and Cerrado, in the State of São Paulo, Brazil. The land use and land cover were mapped between 1971 and 2008 and landscape metrics were calculated to provide a spatiotemporal analysis of the forest structure and the expansion of the croplands...
December 5, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27930868/laser-surface-pattering-of-titanium-for-improving-the-biological-performance-of-dental-implants
#12
Christoph Zwahr, Denise Günther, Tina Brinkmann, Nikolai Gulow, Steffen Oswald, Marzellus Grosse Holthaus, Andrés Fabián Lasagni
Direct laser interference patterning (DLIP) is used to produce periodic line-like patterns on titanium surfaces. An Nd:YAG laser operating at 532 nm wavelength with a pulse duration of 8 ns is used for the laser patterning process. The generated periodic patterns with spatial periods of 5, 10, and 20 µm are produced with energy densities between 0.44 and 2.6 J cm(-)(2) with a single laser pulse. With variation of energy density, different shapes of the arising topography are observed due to the development of the solidification front of the molten material at the maxima positions...
December 8, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27930830/mutual-diffusion-driven-nmr-a-new-approach-for-the-analysis-of-mixtures-by-spatially-resolved-nmr-spectroscopy
#13
Christian F Pantoja, Jose A Bolaños, Andrés Bernal, Julien Wist
We introduce a new approach for resolving the NMR spectra of mixtures that relies on the mutual diffusion of dissolved species when a concentration gradient is established within the NMR tube. This is achieved by cooling down a biphasic mixture of triethylamine and deuterated water below its mixing temperature, where a single phase is expected. Until equilibrium is reached, a gradient of concentration, from "pure" triethylamine to "pure" water, establishes within the tube. The amount of time required to reach this equilibrium is controlled by the mutual diffusion coefficient of both species...
December 8, 2016: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/27930750/spatial-chemical-stimulation-control-in-microenvironment-by-microfluidic-probe-integrated-device-for-cell-based-assay
#14
Masayuki Horayama, Kenta Shinha, Kazuya Kabayama, Teruo Fujii, Hiroshi Kimura
Cell-cell interactions play an important role in the development and function of multicellular organisms. To investigate these interactions in detail, it is necessary to evaluate the behavior of a cell population when the minimum number of cells in the population is stimulated by some chemical factors. We propose a microfluidic device integrated with microfluidic probe (MFP) functionality; this device is capable of imparting a chemical stimulus to cells within a microenvironment, for cell-based assays. The device contains MFP channels at the walls of the cell culture microchannels, and it can control a localized chemical stimulation area at the scale of a single cell to a few cells using MFP fluid control in a microspace...
2016: PloS One
https://www.readbyqxmd.com/read/27930675/spatiotemporal-analysis-of-the-2014-ebola-epidemic-in-west-africa
#15
Jantien A Backer, Jacco Wallinga
In 2014-2016, Guinea, Sierra Leone and Liberia in West Africa experienced the largest and longest Ebola epidemic since the discovery of the virus in 1976. During the epidemic, incidence data were collected and published at increasing resolution. To monitor the epidemic as it spread within and between districts, we develop an analysis method that exploits the full spatiotemporal resolution of the data by combining a local model for time-varying effective reproduction numbers with a gravity-type model for spatial dispersion of the infection...
December 2016: PLoS Computational Biology
https://www.readbyqxmd.com/read/27929470/high-density-electroencephalographic-acquisition-in-a-rodent-model-using-low-cost-and-open-source-resources
#16
Andrzej Z Wasilczuk, Alexander Proekt, Max B Kelz, Andrew R McKinstry-Wu
Advanced electroencephalographic analysis techniques requiring high spatial resolution, including electrical source imaging and measures of network connectivity, are applicable to an expanding variety of questions in neuroscience. Performing these kinds of analyses in a rodent model requires higher electrode density than traditional screw electrodes can accomplish. While higher-density electroencephalographic montages for rodents exist, they are of limited availability to most researchers, are not robust enough for repeated experiments over an extended period of time, or are limited to use in anesthetized rodents...
November 26, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27929466/spatial-and-temporal-analysis-of-active-erk-in-the-c-elegans-germline
#17
Amanda L Gervaise, Swathi Arur
The evolutionarily conserved extracellular signal transducing RTK-RAS-ERK pathway is an important kinase-signaling cascade that controls multiple cellular and developmental processes principally via activation of ERK, the terminal kinase of the pathway. Tight regulation of ERK activity is essential for normal development and homeostasis; overly active ERK results in excessive cellular proliferation, while underactive ERK causes cell death. C. elegans is a powerful model system that has helped characterize the function and regulation of RTK-RAS-ERK signaling pathway during development...
November 29, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27929456/high-throughput-detection-of-respiratory-pathogens-in-animal-specimens-by-nanoscale-pcr
#18
Laura B Goodman, Renee R Anderson, Marcia Slater, Elen Ortenberg, Randall W Renshaw, Brittany D Chilson, Melissa A Laverack, John S Beeby, Edward J Dubovi, Amy L Glaser
Nanoliter scale real-time PCR uses spatial multiplexing to allow multiple assays to be run in parallel on a single plate without the typical drawbacks of combining reactions together. We designed and evaluated a panel based on this principle to rapidly identify the presence of common disease agents in dogs and horses with acute respiratory illness. This manuscript describes a nanoscale diagnostic PCR workflow for sample preparation, amplification, and analysis of target pathogen sequences, focusing on procedures that are different from microliter scale reactions...
November 28, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27929156/correlation-of-annealing-time-with-crystal-structure-composition-and-electronic-properties-of-ch3nh3pbi3-xclx-mixed-halide-perovskite-films
#19
Maryline Ralaiarisoa, Yan Busby, Johannes Frisch, Ingo Salzmann, Jean-Jacques Pireaux, Norbert Koch
Using 3D imaging with time-of-flight secondary ion mass spectrometry (ToF-SIMS) complemented by grazing-incidence X-ray diffraction (GIXRD), we spatially resolve changes in both the composition and structure of CH3NH3I3-xClx perovskite films on conducting polymer substrates at different annealing stages, in particular, before and after complete perovskite crystallization. The early stage of annealing is characterized by phase separation throughout the entire film into domains with perovskite and domains with a dominating chloride-rich phase...
December 8, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27929036/optimal-generation-of-spatially-coherent-soft-x-ray-isolated-attosecond-pulses-in-a-gas-filled-waveguide-using-two-color-synthesized-laser-pulses
#20
Cheng Jin, Kyung-Han Hong, C D Lin
We numerically demonstrate the generation of intense, low-divergence soft X-ray isolated attosecond pulses in a gas-filled hollow waveguide using synthesized few-cycle two-color laser waveforms. The waveform is a superposition of a fundamental and its second harmonic optimized such that highest harmonic yields are emitted from each atom. We then optimize the gas pressure and the length and radius of the waveguide such that bright coherent high-order harmonics with angular divergence smaller than 1 mrad are generated, for photon energy from the extreme ultraviolet to soft X-rays...
December 8, 2016: Scientific Reports
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